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Novel vaccine adjuvant and application

A new type of vaccine adjuvant and vaccine adjuvant technology, which is applied in the field of SPO1 adjuvant, oil-in-water vaccine adjuvant and its preparation, achieves the effect of low cost, suitable for mass production industrialization, and avoiding cross-infection

Active Publication Date: 2012-03-14
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biggest obstacle in the development of mucosal and transdermal non-injectable immunization vaccines is to find a safe and efficient vaccine adjuvant system

Method used

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  • Novel vaccine adjuvant and application
  • Novel vaccine adjuvant and application
  • Novel vaccine adjuvant and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Embodiment 1: the preparation of novel vaccine adjuvant

[0098] Squalene 5.0g

[0099] Polyoxyethylene castor oil 0.3g

[0100] Polyether 0.3g

[0101] Take the raw materials and use a homogenizer to prepare colostrum. The homogenization conditions are: rotating speed 6000rpm, time 4 minutes; the particle size of the colostrum is x 10 =1612.39nm,x 50 =1969.24nm,x 90 =2401.49nm, SMD=1946.88nm, VMD=1992.92nm; homogeneous colostrum: the homogeneous condition is a pressure of 5000psi, 6 cycles, and the particle size is measured as x 10 =197.10nm,x 50 =240.37nm,x 90=292.69nm, SMD=237.53nm, VMD=243.16nm; homogeneous again, homogeneous condition: pressure 10000psi, 7 cycles, measure its particle size as x 10 =125.12nm,x 50 =155.54nm,x 90 = 193.14nm, SMD = 153.30nm, VMD = 157.78nm; after preparing a milky white vaccine adjuvant preparation, add deionized water as buffer to 100ml, filter and sterilize with a 0.22μm filter to obtain the product.

Embodiment 2

[0102] Embodiment 2: the preparation of novel vaccine adjuvant

[0103] Squalene 7.0g

[0104] Polyoxyethylene castor oil 2.0g

[0105] Polyether 1.5g

[0106] Take the raw materials and use a homogenizer to prepare colostrum. The homogenization conditions are: ultrasonic to prepare colostrum, ultrasonic 7 times, 45s / time, the particle size of colostrum is x 10 =1623.75nm,x 50 =1958.31nm,x 90 =2400.35nm, SMD=1948.22nm, VMD=1989.54nm; homogeneous colostrum: homogeneous condition is pressure 6000psi, 7 cycles, measure its particle size as x 10 =197.10nm,x 50 =240.37nm,x 90 =292.69nm, SMD=237.53nm, VMD=243.16nm; homogeneous again, homogeneous condition: pressure 12000psi, 7 cycles, measure its particle size as x 10 =126.13nm,x 50 =156.58nm,x 90 = 194.15nm, SMD = 154.36nm, VMD = 158.79nm, after preparing milky white vaccine adjuvant preparations, add physiological saline as the buffer to 100ml, filter and sterilize with a 0.22μm filter to obtain the final product.

Embodiment 3

[0107] Embodiment 3: the preparation of novel vaccine adjuvant

[0108] Squalene 3.0g

[0109] Polyoxyethylene castor oil 4.0g

[0110] Polyether 3.5g

[0111] Take the raw materials and use a homogenizer to prepare colostrum. The homogenization conditions are: rotating speed 8000rpm, time 3min; the particle size of colostrum is x 10 =1620.79nm,x 50 =1968.33nm,x 90 =2410.65nm, SMD=1950.12nm, VMD=1987.51nm; homogeneous colostrum: the homogeneous condition is a pressure of 7000psi, 5 cycles, and the particle size is measured as x 10 =193.10nm,x 50 =238.55nm,x 90 =290.66nm, SMD=234.53nm, VMD=240.36nm; homogeneous again, homogeneous condition: pressure 14000psi, 6 cycles, measure its particle size as x 10 =123.82nm,x 50 =150.54nm,x 90 = 190.74nm, SMD = 149.30nm, VMD = 157.78nm, after preparing milky white vaccine adjuvant preparation, select phosphate buffer solution to add to 100ml, filter and sterilize with 0.22μm filter to get final product.

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Abstract

The invention discloses a novel vaccine adjuvant (also called as SPO1 vaccine adjuvant) and a preparation method thereof. The vaccine adjuvant disclosed by the invention mainly comprises squalane, polyoxyethylene castor oil and polyether, and can realize immunization through injection or nasal spray, transdermal non-injection and other approaches; and the vaccination through non-injection has the characteristics of low cost, convenience for administration, avoidance of cross infection and high safety. The SPO1 vaccine adjuvant disclosed by the invention can be used for preparing vaccines along with inactivated whole pathogens, extracted components of cracked pathogen, bacterial vesicles and capsular polysaccharide or polysaccharide-binding protein, recombinant protein, recombinant VLP (Virus-like Particle), DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) and other different types of antigens, can be suitable for people in different age groups and preparation of different animal vaccines and immunization, has the advantages of no complex structure, simple process, convenience for preparation and sterilization, low cost and suitability for mass production and industrialization.

Description

technical field [0001] The present invention relates to a vaccine adjuvant and its preparation method, in particular to a novel oil-in-water vaccine adjuvant and its preparation method, also known as a SPO1 adjuvant, which is used for the preparation of vaccines with different types of antigens and for different Vaccine adjuvants required for age group and animal immunization. Background technique [0002] With the rapid development of modern biotechnology, split vaccines, recombinant subunit vaccines, anti-idiotypic antibody vaccines, nucleic acid vaccines and synthetic peptide vaccines have been developed. These vaccine antigens have high purity, relatively small molecular weight, and low adverse reactions to vaccines. However, the immunogenicity and immunoprotection of these antigens are generally weak. In order to improve the effectiveness of the antigens, adjuvants must be added to the antigens to enhance their ability to induce immunity ability to respond. [0003] T...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K9/107A61K48/00A61P37/02
Inventor 王希良刘春环唐冲罗德炎杨鹏辉赵忠鹏王铖邢丽刘永祥
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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