25 results about "Polysaccharide binding" patented technology

A powder or other composition comprising non-spherical, concave microparticles comprising cross-linked starch or polysaccharides, such as plant polysaccharides, in combination with an alginate. Methods for making this powder and methods of using the powder for hemostasis.

The straw in-situ carbonization process comprises the following steps that 1, moisture-proof treatment needs to be conducted on the ground, and straw is stacked for treatment; 2, the stacked straw is smashed, wood rotting fungi or extracts of the wood rotting fungi are sprayed, and uniform spraying is achieved through turning and throwing of a turning and throwing device; step 3, spraying sea mudstone powder on the basis of the step 2, and turning and throwing the sea mudstone powder; 4, the steps 2 and 3 are repeated, the sea mud rock releases far infrared fluctuation of about 10 microns, the far infrared fluctuation and water in the straw (or water in the stack) form a resonance effect, more electrons are excited, polysaccharide binding bonds of plant fibers are promoted to be broken, the effect of catalyzing the straw fermentation reaction is achieved, the fermentation reaction is carried out at the temperature interval of 50-65 DEG C, straw carbonization reaction is carried out, and the straw carbonization reaction is carried out at the temperature interval of 50-65 DEG C; the straw carbonization is realized. The quick-acting fertilizer capacity of the straws is not obviously lost in the carbonization process. The straw forms a fertilizer to form a small molecular carbon fertilizer, which is beneficial to soil absorption and improves the variety and quantity of soil microorganisms.

The invention relates to a method for activating bacterial capsular polysaccharide, and belongs to the technical field of vaccine preparation. According to the method, bacterial capsular polysaccharide is activated by adopting a TEMPO-NCS method in a mixed phase reaction system formed by water and an organic solvent (preferably acetonitrile or DMSO), the volume fraction of the organic solvent is 10%-60% (preferably 20%-40%, further preferably 30%), and the balance of the solvent is water. The bacterial capsular polysaccharide activation method provided by the invention is suitable for various bacteria such as haemophilus influenzae type b (Hib), pneumococcus and the like, the prepared activated polysaccharide can be used for being conjugated with carrier protein to prepare a polysaccharide conjugate, and the obtained polysaccharide conjugate has better immunogenicity and improved safety and can be used for preparing a polysaccharide conjugate vaccine.

The invention belongs to the technical field of synthesis of polysaccharide conjugate vaccines, and relates to a proteus mirabilis O3a O-antigen tetrasaccharide repeating unit containing an adamantane skeleton, an antigen and a polysaccharide conjugate vaccine. The active ingredients of the polysaccharide conjugate vaccine provided by the invention comprise a subject and an object, and the subject and the object are mutually combined through non-covalent interaction; the subject comprises a compound of beta-cyclodextrin and carrier protein, and the guest comprises maltotriose containing an adamantane skeleton or a proteus mirabilis O3a O-antigen containing an adamantane skeleton. The proteus mirabilis O3a O-antigen tetrasaccharide repeating unit containing the adamantane skeleton is successfully synthesized with high stereoselectivity and excellent yield by adopting a [2 + 1 + 1] graded assembly strategy, and the research can lay a firmer theoretical and practical foundation for research and development of proteus mirabilis resistant vaccines.

The present invention relates to the technical field of biologics, and in particular to a method for preparing a Streptococcus pneumoniae capsular polysaccharide. The present invention provides a method for preparing a Streptococcus pneumoniae capsular polysaccharide. The method comprises: using β-propiolactone to treat a Streptococcus pneumoniae fermentation culture, separating and collecting a supernatant, purifying the supernatant, and collecting a capsular polysaccharide. In the present invention, β-propiolactone is used to treat the Streptococcus pneumoniae fermentation culture, thereby reducing residual impurities such as proteins, and achieving relatively high polysaccharide yield; in addition, the β-propiolactone is susceptible to hydrolysis, and the hydrolysate is non-toxic and harmless and has higher safety. The Streptococcus pneumoniae capsular polysaccharide prepared by the method of the present invention have quality control indexes meeting requirements, and can be used for preparing a Streptococcus pneumoniae capsular polysaccharide vaccine and a polysaccharide conjugate vaccine.

The invention discloses a preparation method of a proteoglycan protein conjugate carrying a specified connection site. The method comprises the following steps: carrying out recombinant expression on a recombinant tetanus toxin heavy chain C fragment rTTHc containing a Sortase A 7M ligase recognition sequence, introducing O, O '-(ethane-1, 2-diyl) bis (hydroxylamine) as a nucleophilic reagent into the C tail end of the rTTHc under the action of ligase Sortase A 7M, and carrying out specific condensation reaction on the C tail end of the rTTHc as a unique chemical reaction site and polysaccharide carrying an aldehyde group functional group to obtain the tetanus toxin heavy chain C fragment rTTHc. Therefore, polysaccharide protein conjugates with definite polysaccharide and protein connection sites are produced. According to the invention, the problem that the key antigen epitope of the carrier protein and the heterogeneity of the product are possibly damaged by the traditional random modification strategy is solved, the antigen epitope of the carrier protein is prevented from being damaged to a great extent, and a reliable technical scheme is provided for preparing the polysaccharide conjugate vaccine with high quality and high immunogenicity.

The invention discloses an Escherichia coli chassis strain for N-glycosylation, an Escherichia coli N-glycosylation double-plasmid expression platform and application of the Escherichia coli N-glycosylation double-plasmid expression platform, and belongs to the technical field of biotechnology and synthetic biology. The Escherichia coli chassis strain is an Escherichia coli chassis strain WCB1 obtained by directionally knocking out a waaL gene and a wbbH-L gene cluster of Escherichia coli W3110 by utilizing targeted cleavage of a CRISPR / Cas9 system and homologous recombination of a lambda-Red system; the Escherichia coli N-glycosylation double-plasmid expression platform comprises an Escherichia coli chassis strain WCB1; a first plasmid comprising an O-PS gene cluster; and the second plasmid comprises a coding gene of N-glycosyl transferase PglB and a coding gene of carrier protein CRM197. According to the N-glycosylation plasmid expression platform disclosed by the invention, APEC polysaccharide conjugate vaccines aiming at different serotypes can be produced by replacing different O-PS expression plasmids.

The invention belongs to the technical field of biological medicines, and discloses an anti-tumor polypeptide composition as well as a preparation method and application thereof. The composition is prepared from the following components in parts by weight: 10 to 30 parts of mucopolysaccharide combined hybrid peptide (polypeptide A) extracted from the body wall of the sea cucumber, 5 to 15 parts of angiogenesis inhibiting peptide RGD-4C, 5 to 10 parts of tumor penetrating peptide iRGD, 3 to 8 parts of thymosin alpha 1, 2 to 6 parts of ganoderma lucidum polysaccharide and 1 to 4 parts of vitamin C palmitate. Wherein the polypeptide A is prepared by a process comprising specific ultrasonication, ultrafiltration, ion exchange chromatography and reversed-phase liquid chromatography purification. The invention also provides a preparation method of the composition, which comprises the steps of weighing, sterile mixing, grinding and sub-packaging, and can be further prepared into dosage forms such as freeze-dried powder injection and the like. The composition can be used for preparing medicines for treating solid tumors such as lung cancer, breast cancer, colorectal cancer, liver cancer and the like through synergism of multiple components and multiple target points, and can be combined with chemotherapeutic medicines such as paclitaxel and the like.

A manufacturing process comprising vacuum assisted extraction coupled to ultrasound treatment of food by-products to obtain a controlled release agent comprising polysaccharides and polysaccharide bon

To provide an improved multivalent pneumococcal conjugate composition and a vaccine containing the composition.SOLUTION: A multivalent pneumococcal conjugate composition contains 22 to 27 different kinds of pneumococcal capsular polysaccharide-protein conjugates, wherein each pneumococcal capsular polysaccharide-protein conjugate includes a protein carrier conjugated into capsular polysaccharide derived from a different serotype of streptococcus pneumoniae, and a streptococcus pneumoniae serotype is selected from 1, 3, 4, 5, 6A, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15A, 15B, 15C, 18C, 19A, 19F, 22F, 23A, 23B, 23F, 24F, 33F and 35B.SELECTED DRAWING: None

The invention provides a streptococcus suis serotype 2 virulent strain resistant to multiple antibiotics and application, and belongs to the technical field of vaccine preparation, the streptococcus suis serotype 2 resistant to multiple antibiotics is preserved in China Center for Type Culture Collection on December 6, 2024, the preservation number is CCTCC NO: M20242747, the classification name is streptococcus suis serotype 2 S068, the prepared streptococcus suis serotype 2 polysaccharide conjugate vaccine is applied, and the streptococcus suis serotype 2 polysaccharide conjugate vaccine can be used for preparing the streptococcus suis serotype 2 polysaccharide conjugate vaccine. The protection rate is higher, and good adaptability, high efficiency and safety in a complex clinical environment are shown.

The present application relates to a kind of methods for activating bacterial capsule polysaccharide, belong to vaccine preparation technical field.The present application activates bacterial capsule polysaccharide using TEMPO-NCS method, it is carried out in the mixed phase reaction system formed by water and organic solvent (preferably acetonitrile or DMSO), wherein, the volume fraction of organic solvent is 10%~60% (wherein preferably 20%~40%, further preferably 30%), the rest solvent is water.The bacterial capsule polysaccharide activation method provided by the present application is suitable for a variety of bacteria such as Haemophilus influenzae type b (Hib), pneumococcus, etc., and the activated polysaccharide prepared can be used to conjugate with carrier protein to prepare polysaccharide conjugate, and the polysaccharide conjugate obtained has better immunogenicity and improved safety, and can be used to prepare polysaccharide conjugate vaccine.

The invention discloses a method for screening a surfactant for extracting allium macrostemon polysaccharide based on molecular docking simulation. The method comprises the following steps: (1) treating an allium macrostemon raw material; (2) constructing a surfactant model; (3) constructing an allium macrostemon polysaccharide monosaccharide model; (4) simulating and screening molecular docking; and (5) carrying out experimental verification on the candidate surfactant. According to the method, the surfactant is screened in advance through molecular docking simulation, the number of candidate agents is reduced to 3-5, the screening period is shortened to 1-2 weeks, and the problem that a traditional trial and error method consumes time and labor is thoroughly solved; the optimal extraction rate of the surfactant reaches 26.18-26.52%, and is improved by 51.91-77.87% compared with the traditional hot water extraction under the same extraction time; based on intermolecular structure complementarity and interaction energy quantification screening, the randomness of a traditional trial and error method is avoided, the docking fraction is directly associated with the surfactant-polysaccharide binding capacity, and the screened optimal surfactant is stably bound with allium macrostemon polysaccharide.

The invention provides a grinding tool for silicon carbide ceramic machining and a preparation method of the grinding tool. The grinding tool mainly comprises the following raw materials of a polysaccharide binding agent, a grinding material, a reinforcing phase, a dispersing agent and a phase change material. The phase change material is compounded polyethylene glycol with two or more average molecular weights. Two or more polyethylene glycol with the average molecular weight are compounded, so that an abrasive particle falling mechanism can be optimized, and the overall mechanical strength of the grinding tool can be maintained by enhancing the stability of a polysaccharide binding agent framework. According to the invention, two or more polyethylene glycol with average molecular weight are compounded, so that the grinding tool can trigger controllable self-sharpening at a specific grinding temperature; when the grinding temperature causes the temperature of the polysaccharide binding agent to rise to a set phase change temperature interval, the passivated abrasive particles are promoted to fall off, and the abrasive particles can be firmly held under the normal working condition.

A use of a Sip protein in the preparation of a capsular polysaccharide conjugate vaccine, and a conjugate comprising the Sip protein and a preparation method therefor and a use thereof, relating to the technical field of polysaccharide conjugate vaccines. The present invention provides the use of a Sip protein in the preparation of a capsular polysaccharide conjugate vaccine. The capsular polysaccharide comprises the group B streptococcus (GBS) capsular polysaccharide. Also provided are the conjugate comprising the Sip protein and the preparation method therefor and the use thereof. By using the technical solution of the present invention, an antigen can be transformed from a T-cell independent antigen to a T-cell dependent antigen, and an antibody that generates specificity can be induced to generate a good immunoprotective effect; moreover, immunogenicity is stable, and good immunological memory can be generated; and furthermore, the protection scope of the prepared capsular polysaccharide conjugate vaccine against infections caused by different serotypes of GBS can be expanded, so that the vaccine can have multiple protections, and has good prospects for application in infections caused by uncommon serotypes of GBS.

The present application belongs to the technical field of biological medicine, and relates to a kind of Parachlorella sp. Polysaccharide and its preparation method and application. The present application obtains a kind of uniform Parachlorella sp. Polysaccharide NGP-1 by water extraction, alcohol precipitation, column chromatography separation to Parachlorella sp.;Combining high performance liquid chromatography, methylation combined gas chromatography-mass spectrometry, nuclear magnetic resonance and other methods to determine its structural characteristics. The obtained NGP-1 can promote macrophage proliferation, enhance phagocytic ability, improve active oxygen level, induce nitric oxide and cytokine production, and up-regulate the expression of corresponding mRNA, and has significant immune enhancing activity. The Parachlorella sp. Polysaccharide NGP-1 prepared by the present application can be used as a natural immune enhancer, which widens the application range of Parachlorella sp. And has broad application prospect.

The invention discloses lycium ruthenicum polysaccharide as well as a preparation method and application thereof, and relates to the technical field of deep processing and application of natural products. According to the method disclosed by the invention, plant cell walls are destroyed under mild conditions by utilizing an enzyme method through an enzyme method-assisted aqueous two-phase extraction technology so as to release polysaccharide, and the polysaccharide is efficiently separated and purified by combining an aqueous two-phase system, so that the yield and the activity retention rate are improved. The high selectivity of an enzyme method is combined with the mild distribution characteristic of an aqueous two-phase system, so that the use of an organic solvent can be reduced, the environmental pollution can be reduced, and the biological activity of the lycium ruthenicum polysaccharide in the prevention and treatment of diseases related to retinal endothelial cell injury can be reserved. The lycium ruthenicum polysaccharide prepared by the method can be used for preparing auxiliary treatment preparations for ophthalmic diseases such as diabetic retinopathy and macular degeneration.

The invention discloses a thiolated polysaccharide combined probiotic microcapsule as well as a preparation method and application thereof, and belongs to the technical field of probiotic microcapsules. Comprising the following steps: (1) mixing and incubating a probiotic suspension and a 2-imino thioalkane solution to obtain thiolated probiotics; and (2) adding the thiolated probiotic suspension into the thiolated polysaccharide solution, carrying out oscillation reaction, and centrifuging to remove unattached probiotics. Polysaccharide is firstly subjected to thiolation modification, and primary amino groups on the surfaces of bacteria are converted into free mercaptan under a cell compatibility condition through a simple one-step imide ester reaction, so that the free mercaptan can spontaneously form disulfide bonds to perform chemical bonding under a catalyst-free condition, and the primary amino groups on the surfaces of the bacteria are converted into the free mercaptan. The microcapsule prepared from the modified polysaccharide and the probiotics can also be combined with mucoprotein rich in the mucous layer of the intestinal tract to generate a catalyst-free thiol-disulfide exchange reaction, and colonization is carried out at the mucous layer in the intestinal tract, so that better intestinal disease treatment and probiotic benefits are generated.

Provided is a granule for suspension, which is a pharmaceutical composition including: a compound represented by formula 1:or a pharmaceutically acceptable salt or solvate thereof, a binding agent; and a dispersant. The binding agent includes a binding agent selected from the group consisting of a cellulose binding agent, a povidone binding agent, a vinyl alcohol binding agent, and a thickening polysaccharide binding agent. The dispersant is selected from the group consisting of an inorganic salt dispersant, an ether dispersant, a starch dispersant, a cellulose dispersant, and a fatty acid ester salt dispersant.

This application relates to the technical field of vaccine conjugates, specifically to a method for preparing a polysaccharide conjugate of group A meningococcal capsular polysaccharide, including the preparation of polysaccharide derivatives, mixing ratio, conjugation reaction, aging treatment, ultrafiltration and sterilization steps. This application eliminates the pH adjustment and conjugate purification steps in the activation process by optimizing the process parameters, but the quality of the harvested polysaccharide conjugate still meets the national standards, thereby simplifying the preparation process of polysaccharide conjugates and shortening the preparation cycle of polysaccharide conjugates.

The present invention relates to a method for extracting acidic Tremella polysaccharide using bilirubin-modified chitosan, the method comprising the steps of: (1) preparing a bilirubin-modified chitosan material; preparing a Tremella extract; (2) passing the Tremella extract through a bilirubin-modified chitosan material chromatography column, whereby the acidic Tremella polysaccharide binds to amino groups on the surface of the bilirubin and is adsorbed on the surface of the modified chitosan, thereby separating the acidic Tremella polysaccharide from other components; then adding a strong ion salt solution to separate the acidic Tremella polysaccharide from the modified chitosan, and filtering the mixture using a filter membrane to obtain an acidic Tremella polysaccharide solution. The method for extracting acidic Tremella polysaccharide using bilirubin-modified chitosan provided by the present invention has a simple process, low production cost, high product quality, and is suitable for further promotion and application.

The invention discloses a preparation method of an insect protein and edible fungus polysaccharide compound and application of the insect protein and edible fungus polysaccharide compound in functional food, and belongs to the technical field of food biology.According to the method, a supercritical extraction technology is adopted for extracting tenebrio molitor protein, and the extraction rate is larger than 90%; the morchella polysaccharide is extracted by adopting an ultrasonic-assisted extraction technology, and the extraction rate is 12-18%; protein and polysaccharide are combined through an electrostatic self-assembly technology, a nanoscale compound with the average particle size of 200-250 nm is formed, the compound has remarkable immunoregulation and antioxidant activity, the promoting effect on splenic lymphocyte proliferation is improved by 1.8-2.3 times compared with that of a single component, the bioavailability is improved by 2.8 times or above, and the preparation method is simple and easy to implement. The method can be applied to preparation of functional foods such as oral liquid and capsules.

The invention belongs to the technical field of biological medicine, and relates to micrococcus sp. Polysaccharide as well as a preparation method and application thereof. The preparation method comprises the following steps: performing water extraction, alcohol precipitation and column chromatography separation on the nannochloropsis to obtain uniform nannochloropsis polysaccharide NGP-1; the structural characteristics of the compound are determined by combining high performance liquid chromatography, methylation combined with gas chromatography-mass spectrometry, nuclear magnetic resonance and other methods. The obtained NPG-1 can promote proliferation of macrophages, enhance phagocytic ability, improve the active oxygen level, induce generation of nitric oxide and cell factors and up-regulate expression of corresponding mRNA, and has remarkable immunological enhancement activity. The prepared NPG-1 can be used as a natural immunopotentiator, the application range of the NPG-1 is widened, and the NPG-1 has a wide application prospect.

This invention discloses an immunogenic preparation method for high-titer anti-pneumococcal capsular polysaccharide rabbit serum based on polysaccharide conjugates. The method involves diluting the pneumococcal capsular polysaccharide conjugate stock solution with physiological saline, adding Freund's adjuvant at a 1:1 volume ratio, emulsifying to form an antigen mixture, and using healthy New Zealand white rabbits as immunization animals. The antigen is administered via subcutaneous injection at multiple sites, with appropriate immunization doses and intervals, and repeated immunizations are performed. This invention uses polysaccharide conjugates instead of traditional bacterial cells as the immunogen. Polysaccharide conjugates combine polysaccharides with protein carriers, thereby enhancing their immunogenicity and stimulating a stronger immune response, including higher levels of antibodies and memory cells. Furthermore, polysaccharide conjugates typically have higher purity than bacterial cells, which are prone to producing impurities other than the target antibody, affecting the quality of the antiserum and increasing the workload of subsequent purification.

The invention belongs to the technical field of biology, and particularly relates to a carbohydrate binding module and application thereof. The invention discloses a carbohydrate binding module for the first time, and the carbohydrate binding module can be bound with insoluble polysaccharide and can be used for preparing and / or preparing tag protein, immobilizing target protein and / or preparing an immobilized compound.