67 results about "Polysaccharide binding" patented technology

The present invention relates to biodegradable biocompatible polyketals, methods for their preparation, and methods for treating animals by administration of biodegradable biocompatible polyketals. In one aspect, a method for forming the biodegradable biocompatible polyketals comprises combining a glycol-specific oxidizing agent with a polysaccharide to form an aldehyde intermediate, which is combined with a reducing agent to form the biodegradable biocompatible polyketal. The resultant biodegradable biocompatible polyketals can be chemically modified to incorporate additional hydrophilic moieties. A method for treating animals includes the administration of the biodegradable biocompatible polyketal in which biologically active compounds or diagnostic labels can be disposed. The present invention also relates to chiral polyketals, methods for their preparation, and methods for use in chromatographic applications, specifically in chiral separations. A method for forming the chiral polyketals comprises combining a glycol-specific oxidizing agent with a polysaccharide to form an aldehyde intermediate, which is combined with a suitable reagent to form the chiral polyketal. A method for use in chiral separations includes the incorporation of the chiral polyketals in the mobile phase during a chromatographic separation, or into chiral stationary phases such as gels. The present invention further relates to chiral polyketals as a source for chiral compounds, and methods for generating such chiral compounds.

Magnetic nanoparticles and a method for producing the same are disclosed. A carboxylated polysaccharide with various functions such as chelating and ion exchange is covalently bound on the surfaces of magnetic nanoparticles. The polysaccharide-bound magnetic nanoparticles are highly stable, well dispersed, and have many advantages of high adsorption capacity, fast adsorption rate and easy magnetically manipulation. Hence, the polysaccharide-bound magnetic nanoparticles are applied to adsorb many ionic substances, and further act as an adsorbent for wastewater treatment or biochemical separation, a carrier for drugs or gene site-directed delivery, and a magnetic resonance imaging (MRI) contrast agent.

A photoreactive polysaccharide which comprises a polysaccharide bound to a glycidyl ester via a covalent bond, a photocrosslinked-polysaccharide prepared by using the photoreactive polysaccharide, and medical products comprising the photocrosslinked-polysaccharide.

The invention provides a multivalent pneumococcus capsular polysaccharide-protein conjugated composition and a preparation method thereof. The conjugated composition is formed by covalent linkage of multivalent pneumococcus capsular polysaccharides of 14 different serotypes and carrier protein, wherein the 14 serotypes include 1, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, 22F, 23F and 33F. The conjugated composition has good adsorption effect and good stability, has multiple immunogenicity and protective performance against invasion of the pneumococcus of 14 serotypes, is superior to on-sale low-valent pneumonia compositions, and the immune response of the conjugated composition disclosed by the invention is higher than that of an uncombined composition. The inoculating injection frequency can be reduced by using the multivalent pneumococcus capsular polysaccharide conjugate vaccine containing the conjugated composition, the immune process can be simplified, and diseases of human and animals caused by the 14 serotypes of pneumococcal bacteria can be effectively prevented. The conjugated composition has wider coverage and better immune effect.

Capsular polysaccharides containing multiple sialic acid residues, particularly the Group B polysaccharide of Neisseria meningitidis, are modified by chemical reaction to randomly introduce pendant reactive residues of heterobifunctional linker molecules to the polysaccharide backbone. The capsular polysaccharide is deacetylated and the heterobifunctional linker molecule is reacted with the deacetylated material and any residual amino groups are blocked by reaction with alkyl acid anhydride. The introduction of the linker molecules to the polysaccharide chain between the termini enables the polysaccharide to be linked to a carrier molecule, such as a protein, to enhance the immunogenicity of the polysaccharide. The conjugate molecule may be formulated as an immunogenic composition for raising antibodies in a host to the polysaccharide.

A camptothecin derivative comprising a compound of the formula [I]:wherein R1, R2, R3, R4 and R5 are (A) adjacent two groups combine to form alkylene, or both are H, and one of the remaining three groups is -Xn-Alkm-R6, and the other two are H, alkyl or halogen, or (B) adjacent two groups combine to form alkylene, and one of the carbon atoms of said alkylene group is substituted by -Xn-Alkm-R6, and the remaining three groups are H, alkyl or a halogen, and one or two -CH2- of the alkylene in (A) or (B) may optionally be replaced by -O-, -S- or -NH-, X is -O- or -NH-, Alk is alkylene,or -OH, m and n are both 0 or 1, or m is 1 and n is 0, which camptothecin compound is bound to a polysaccharide having carboxyl groups via an amino acid or a peptide, or a pharmaceutically acceptable salt thereof. Said camptothecin derivatives show enhanced antitumor activities but few side effects and are useful as a medicament.

The invention discloses a novel vaccine adjuvant (also called as SPO1 vaccine adjuvant) and a preparation method thereof. The vaccine adjuvant disclosed by the invention mainly comprises squalane, polyoxyethylene castor oil and polyether, and can realize immunization through injection or nasal spray, transdermal non-injection and other approaches; and the vaccination through non-injection has the characteristics of low cost, convenience for administration, avoidance of cross infection and high safety. The SPO1 vaccine adjuvant disclosed by the invention can be used for preparing vaccines along with inactivated whole pathogens, extracted components of cracked pathogen, bacterial vesicles and capsular polysaccharide or polysaccharide-binding protein, recombinant protein, recombinant VLP (Virus-like Particle), DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) and other different types of antigens, can be suitable for people in different age groups and preparation of different animal vaccines and immunization, has the advantages of no complex structure, simple process, convenience for preparation and sterilization, low cost and suitability for mass production and industrialization.

The invention discloses a bacterial polysaccharide O-glycosylation modified recombinant cholera toxin B subunit fusion protein and an application thereof. The present invention provides a conjugate of polysaccharide and protein which is coupled by the bacterial polysaccharide and recombinant cholera toxin B subunit fusion protein. The bacterial polysaccharide is connected with the glycosylation sites of the recombinant cholera toxin B subunit fusion protein in the form of O-glucosidic bond. The experiment indicates that the preparation of the bacterial polysaccharide protein conjugate vaccine from O-glycosylation modified recombinant cholera toxin B subunit fusion protein can enhance the ability of inducing animals to generate anti-polysaccharide antibodies, avoid miscellaneous problems of pathogen culture, enhance the vaccine homogeneity and production efficiency, and reduce the vaccine preparation cost, therefore possessing wide application prospect.

The invention relates to a meningococcal polysaccharide conjugate vaccine soluble microneedle patch and a preparation method thereof. The soluble microneedle patch comprises a soluble microneedle substrate and soluble microneedle bodies, wherein the soluble microneedle substrate is prepared from an aqueous solution of a first water-soluble high-polymer material, and the soluble microneedle bodies are prepared from an aqueous solution of a second water-soluble high-polymer material and a group A+C meningococcal polysaccharide conjugate vaccine; the percentage by weight of the group A+C meningococcal polysaccharide conjugate vaccine in the soluble microneedle bodies is not greater than 8 percent, and is not 0; the second water-soluble high-polymer material is prepared from sodium hyaluronate, dextran and povidone, and the molecular weight of the water-soluble high-polymer material is 7000 to 630000. The soluble microneedle patch has good mechanical properties and solubility, and the effect of regulating the immune response of the vaccine is better than the effect of a subcutaneous injection immunization method under the same condition.

The present invention relates to a hydrogel, a preparation method thereof, and a pH sensor comprising the same, and more particularly, to a hydrogel, which can substitute for conventional pH paper, is continuously usable, can be used in various applications, and reversibly changes color depending on the pH of a sample, and to a pH sensor comprising the same. The hydrogel comprises: a polymer complex wherein a first organic compound containing an aromatic functional group having at least one hydroxyl group bonded thereto is joined to a carboxyl group-containing polysaccharide by a peptide bond; and an organic dye comprising a second organic compound containing an aromatic functional group having at least one hydroxyl group bonded thereto.

The invention describes a method for preparing a meningitis polysaccharide conjugate vaccine. The meningitis polysaccharide conjugate vaccine is developed based on the method. The method comprises the following steps: (1) activating meningococcus polysaccharides by cyanogen bromide, then deriving the meningococcus polysaccharides which are activated by the cyanogen bromide by ethanediamine, finally deriving the polysaccharides by a reagent which is of a structure of succinimidyl ester-R-maleimide; (2), sulfhydrylating carrier proteins; (3) combining the derived meningococcus polysaccharides with the sulfhydrylated carrier proteins. As conjugation bridges between the meningococcus polysaccharides and the carrier proteins are very long, the spatial shielding effect of the carrier proteins on the antigenic epitopes of the polysaccharides is reduced, and the original immunization property of the polysaccharide conjugate vaccine is improved.

The invention provides a method for continuously detecting glucose concentration in a sample, including: (a) providing a biosensor comprising a transducer and a polysaccharide covered on the surface of the transducer; (b) providing a carbohydrate binding protein solution, wherein the carbohydrate binding protein has at least one receptor, and the receptor is capable of binding to the polysaccharide and glucose; (c) mixing a sample and the carbohydrate binding protein solution to form a mixture; (d) contacting the mixture with the biosensor; (e) detecting the amount of carbohydrate binding proteins bound to the polysaccharide by the biosensor, wherein glucose concentration of the sample is inversely proportional to the amount of carbohydrate binding proteins bound to the polysaccharide; and (f) refreshing the surface of the biosensor with a high concentration glucose solution.

The present invention provides a nucleotide molecule, which has a sequence represented by SEQ ID NO.:2, 4 or 6. The present invention further provides a recombinant protein PspA1, PspA2 or PspA3 encoded by the nucleotide molecule of the present application. The present invention further provides a polysaccharide conjugation vaccine, which comprises meningococcal polysaccharide and a carrier protein bound to the meningococcal polysaccharide, wherein the carrier protein is the recombinant protein PspA1, PspA2 or PspA3 encoded by the nucleotide molecule of the present application. The present invention further provides uses of the recombinant proteins PspA1, PspA2 and PspA3 encoded by the nucleotide molecule of the present invention as the carrier protein of the polysaccharide conjugation vaccine. The present invention further provides an expression vector, which comprises the nucleotide molecule of the present invention.

Isolated polypeptides are disclosed comprising an amino acid sequence encoding a monomer of a fibrous polypeptide attached to a heterologous polysaccharide binding domain. Composites comprising same, methods of generating same and uses thereof are all disclosed.

Gels and polymers comprising a polypeptide bound to a polysaccharide are disclosed. Specific polypeptides include, but are not limited to, polypeptides that comprise glutamine or tyrosine residues. Specific polysaccharides include, but are not limited to, chitosan. Gels and polymers of the invention can be used for the in vitro and in situ formation of protein-polysaccharide conjugates. Methods of making polypeptide / polysaccharide gels and polymers are also disclosed.

The invention provides a multivalence pneumococcus capsular polysaccharide-protein conjugate composition and a preparation method thereof. The conjugate composition is prepared from capsular polysaccharides of pneumococcus of 24 different serotypes and a carrier protein in a covalence connection manner, wherein the 24 different serotypes are 1, 2, 3, 4, 5, 6A, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F and 33F. The conjugate composition is good in adsorption effect and stability, has multiple immunogenicity and protection properties aiming at invasion of pneumococcus of the 24 serotypes, and is superior to the low-valence pneumonia composition in the market, and the immune response is higher than that of uncombined compositions. By inoculating a multivalence pneumococcus capsular polysaccharide conjugate vaccine prepared from the conjugate composition, the inoculation injection times can be reduced, the immunization procedure can be simplified, and meanwhile human beings and animals can be effectively prevented from diseases resulted from the pneumococcus of the 24 serotypes, and the conjugate composition is wide in coverage range and good in immune effect.

The invention discloses a single crystal silicon carbide green and efficient polishing tool. The tool comprises the following raw materials of, by weight, 48-75% of an abrasive material, 1-2% of a reinforcing phase, 0.35-0.75% of a dispersing agent and the balance polysaccharide binding agents. A method for preparing the single crystal silicon carbide green and efficient polishing tool with the materials above specifically comprises the following steps of material mixing, curing, polishing tool bonding and polishing tool shaping. The invention further discloses a method for polishing single crystal silicon carbide based on the single crystal silicon carbide green and efficient polishing tool prepared through the method. The tool is in contact with single crystal silicon carbide to generatedry friction, and the oxygen partial pressure range of the ambient atmosphere of a friction region ranges from 20 Pa-100 Pa. According to the technical scheme, the single crystal silicon carbide green and efficient polishing tool successfully prepared through the method is environment-friendly, non-toxic and low in manufacturing and polishing processing cost, and and green and efficient polishingon the surface of the single crystal silicon carbide can be realized.

The invention discloses an edible fungus polysaccharide mixed high-concentration protein beverage and a production method thereof. The edible fungus polysaccharide mixed high-concentration protein beverage comprises, by weight, 0.2-1% of edible fungus polysaccharide crude extract, 2-5% of whey protein isolate and / or soybean protein isolate and the balance of water, with pH of 3-6. The invention provides an edible fungus polysaccharide mixed high-concentration whey protein or soybean protein or whey-soybean protein beverage and a making method thereof. Edible fungus polysaccharides bind with proteins under certain conditions to form a soluble complex; high-concentration proteins are stably dispersed in a solution. The edible fungus polysaccharides can be mixed with a single-protein or dual-protein beverage to provide two nutrients, excellent proteins and polysaccharides; the manufacturing process has low equipment cost and low energy consumption; the edible fungus polysaccharide mixed high-concentration protein beverage is easy to popularize in edible fungus production areas, milk production areas, soybean production areas and other areas.

The invention belongs to the field of classification and purification of natural products, and particularly relates to an amphiphilic temperature-sensitive block polymer based on phenylboronic acid and a preparation method and application thereof. Accord to that invention, a temperature-sensitive block and a phenylboronic acid block are polymerized by use a chain transfer technology to obtain theamphiphilic temperature-sensitive block polymer based on phenylboronic acid, and the obtained block polymer has the functions of ortho-dihydroxy identifying and temperature-sensitivity; according to the invention, the amphiphilic temperature-sensitive block polymer is combined with polysaccharides containing ortho-dihydroxy, polysaccharides with different molecular weights are aggregated and precipitated by adjusting different temperatures, and classification and purification of the polysaccharides are realized. Compared with the current common method, the temperature-controlled precipitationmethod for different fractions of polysaccharide is more green and environment-friendly, and the structure and activity of each polysaccharide sample are not damaged.

A kit for the determination of the ability of a peripheral blood mononuclear cell to bind to a polysaccharide herein provided comprises a detection reagent comprising a fluorescence-labeled immunostimulant polysaccharide; and a reference reagent comprising a fluorescence-labeled material of a polysaccharide different from that used in the fluorescence-labeled immunostimulant polysaccharide, and / or an immunostimulant polysaccharide which is not labeled with any fluorescent material and whose polysaccharide moiety is identical to that included in the fluorescence-labeled immunostimulant polysaccharide. The use of this kit would permit the estimation of the polysaccharide-binding ability of a peripheral blood mononuclear cell in a higher precision of estimation.

The invention discloses a kit for genome DNA extraction and an application of the kit. The kit comprises a lysis solution and an erythrocyte lysis solution, wherein the lysis solution contains 20 to 300mM of Tris-HCl, 20 to 100mM of EDTA, 0.3 to 2M of NaCl, 0.5 to 5 percent of PEG8000 and 0.5 to 10 percent of Triton X-100, and SDS or Tween 20; the erythrocyte lysis solution contains 10 to 100mM ofTris-HCl, 1 to 50mM of EDTA, 10 to 300mM of NaCl and 0.01 to 0.2 percent of Triton X-100. According to the kit, through optimization of the lysis solution and the erythrocyte lysis solution, polysaccharide can be effectively removed, phenol is combined, phenol is prevented from being combined with DNA, the problems of viscosity and turbidity are solved, the kit is suitable for various different samples, and the purity and yield of extracted genome DNA can meet various downstream experiment use requirements.

The invention relates to the technical field of vaccine conjugates, in particular to a preparation method of a group A meningococcus capsular polysaccharide conjugate, which comprises the steps of polysaccharide derivative preparation, mixing proportioning, conjugation reaction, aging treatment, ultrafiltration and sterilization. According to the method, the processes of pH adjustment in the activation process and conjugate purificationare omitted through optimization of all technological parameters, but the quality of the harvested polysaccharide conjugate still meets the national standard, so that the preparation process of the polysaccharide conjugate is simplified, and the preparation period of the polysaccharide conjugate is shortened.

A camptothecin derivative comprising a compound of the formula [I] is disclosed:wherein R1, R2, R3, R4 and R5 are (A) adjacent two groups combine to form alkylene, or both are H, and one of the remaining three groups is -Xn-Alkm-R6, and the other two are H, alkyl or halogen, or (B) adjacent two groups combine to form alkylene, and one of the carbon atoms of said alkylene group is substituted by -Xn-Alkm-R6, and the remaining three groups are H, alkyl or a halogen, and one or two -CH2- of the alkylene in (A) or (B) may optionally be replaced by -O-, -S- or -NH-, X is -O- or -NH-, Alk is alkylene, R6 is -NH2,or -OH, m and n are both 0 or 1, or m is 1 and n is 0, which camptothecin compound is bound to a polysaccharide having carboxyl groups via an amino acid or a peptide, or a pharmaceutically acceptable salt thereof. Said camptothecin derivatives show enhanced antitumor activities but few side effects and are useful as a medicament.

This invention relates to one test method for free polysaccharide content of pneumococcal peritonitis compound in biological technique field, which comprises the following steps: a, fetching same content of the compound and adding ethanol without water and common salt for 70 to 90 hours under -20 degrees then removing upper liquid by anticentripetal and using proper agent to clear deposition to collect upper liquid through filter salt and ethanol; b, using anthranone to test sample one and two to compute free polysaccharide content.

The invention discloses a method for determining a binding rate of a proteoglycan protein binding site in a proteoglycan protein conjugate vaccine. The method comprises the following steps of: (1) carrying out enzymolysis on a standard substance; (2) carrying out solid-phase extraction to enrich a target peptide fragment; (3) performing high performance liquid chromatography tandem mass spectrometry analysis; (4) drawing a standard curve to obtain a standard working curve equation; (5) detecting a sample, and calculating to obtain the concentration of each characteristic peptide fragment in the sample; and (6) calculating a proteoglycan binding rate of each characteristic peptide fragment and a protein residue rate of each site. According to the method, the multi-site quantitative monitoring of the proteoglycan protein binding rate of the proteoglycan protein conjugate vaccine is achieved, the tetanus protein relates to 20 monitoring sites, and the CRM197 relates to 16 monitoring sites; the method not only can detect the residual protein content, but further can evaluate the binding rate of the proteoglycan protein binding site of the proteoglycan protein conjugate vaccine, can beused for researching polysaccharide or proteoglycan protein conjugate vaccines with the same protein residual rate but different activity and toxicity, and can provide a direct monitoring method for improving a proteoglycan protein binding process.

The invention belongs to the technical field of microorganisms, and in particular relates to a special multifunctional growth-promoting bacterium agent for soybeans, which is fermented from the FK19 strain, and the classification of the FK19 strain is named Bacillus megaterium (Bacillus megaterium), preserved on December 27, 2018 In China Center for Type Culture Collection, the deposit number is CCTCC M 2018925. The beneficial effects of the present invention are: the multifunctional growth-promoting bacterium agent of the present invention has obvious promoting effect on soybean growth, has obvious increasing effect on phosphorus content and potassium content of soybean leaves, and can increase soybean yield. Soybean roots will produce soybean lectin, which is a sugar-binding protein or glycoprotein of non-immune origin, which can be combined with polysaccharides of bacteria. The multifunctional growth-promoting bacteria agent of the present invention has soybean lectin affinity, and can Combined, adsorbed on the soybean roots, and then promote the synthesis of more isoflavones in soybeans, mediate the colonization of the bacterial strains in the soybean roots, so as to exert the growth-promoting function of the bacterial agent.

The invention provides a preparation method of a novel polysaccharide conjugate vaccine treating a PEG heterobifunctional reagent as a conjugation bridge. A meningococcal polysaccharide conjugate vaccine is exploited through the method. The method has the following advantages: 1, the respective self-crosslinking of polysaccharides and proteins in traditional methods is avoided, the yield is improved, and the quality control is benefited; and 2, a long PEG chain can increase the distance between the polysaccharides and the proteins, reduce the mutual space shield effect between the polysaccharides and the proteins, and improve the immunogenicity of the polysaccharide conjugate vaccine.

The invention discloses dendrobium officinale polysaccharide and a preparation method thereof. The dendrobium officinale polysaccharide comprises dendrobium officinale roots, the dendrobium officinaleroots are subjected to a water extraction and alcohol precipitation method to extract crude polysaccharide, separation and purification methods such as repeated freezing and thawing, H2O2 decoloration, ultrafiltration, dialysis and the like are combined to respectively obtain two kinds of dendrobium officinale polysaccharide DOP-1 and DOP-2 with uniform molecular weight. Due to the fact that theviscosity of DOP-2 is lower than that of DOP-1, the 2D nuclear magnetic detection related peak response is good, DOP-1 and DOP-2 are smashed after multiple determination, then water at the temperatureof 220 DEG C is added for soaking and heating, heating is stopped when the DOP-1 and the DOP-2 are dispersed, residues in the DOP-1 and the DOP-2 are filtered, a concentrated solution is extracted, clear liquid floating on the concentrated solution is removed through a water extraction and alcohol precipitation method, and then the concentrated solution is precipitated and dried at high temperature to finally obtain the dendrobium officinale polysaccharide, so that the sugar content of the dendrobium officinale is increased, more raw materials are saved in the preparation process, and the reaction rate is effectively increased through hydroxyl free radicals and superoxide anions.