61 results about "Polysaccharide binding" patented technology

Magnetic nanoparticles and a method for producing the same are disclosed. A carboxylated polysaccharide with various functions such as chelating and ion exchange is covalently bound on the surfaces of magnetic nanoparticles. The polysaccharide-bound magnetic nanoparticles are highly stable, well dispersed, and have many advantages of high adsorption capacity, fast adsorption rate and easy magnetically manipulation. Hence, the polysaccharide-bound magnetic nanoparticles are applied to adsorb many ionic substances, and further act as an adsorbent for wastewater treatment or biochemical separation, a carrier for drugs or gene site-directed delivery, and a magnetic resonance imaging (MRI) contrast agent.

The invention discloses a novel vaccine adjuvant (also called as SPO1 vaccine adjuvant) and a preparation method thereof. The vaccine adjuvant disclosed by the invention mainly comprises squalane, polyoxyethylene castor oil and polyether, and can realize immunization through injection or nasal spray, transdermal non-injection and other approaches; and the vaccination through non-injection has the characteristics of low cost, convenience for administration, avoidance of cross infection and high safety. The SPO1 vaccine adjuvant disclosed by the invention can be used for preparing vaccines along with inactivated whole pathogens, extracted components of cracked pathogen, bacterial vesicles and capsular polysaccharide or polysaccharide-binding protein, recombinant protein, recombinant VLP (Virus-like Particle), DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) and other different types of antigens, can be suitable for people in different age groups and preparation of different animal vaccines and immunization, has the advantages of no complex structure, simple process, convenience for preparation and sterilization, low cost and suitability for mass production and industrialization.

The invention relates to a meningococcal polysaccharide conjugate vaccine soluble microneedle patch and a preparation method thereof. The soluble microneedle patch comprises a soluble microneedle substrate and soluble microneedle bodies, wherein the soluble microneedle substrate is prepared from an aqueous solution of a first water-soluble high-polymer material, and the soluble microneedle bodies are prepared from an aqueous solution of a second water-soluble high-polymer material and a group A+C meningococcal polysaccharide conjugate vaccine; the percentage by weight of the group A+C meningococcal polysaccharide conjugate vaccine in the soluble microneedle bodies is not greater than 8 percent, and is not 0; the second water-soluble high-polymer material is prepared from sodium hyaluronate, dextran and povidone, and the molecular weight of the water-soluble high-polymer material is 7000 to 630000. The soluble microneedle patch has good mechanical properties and solubility, and the effect of regulating the immune response of the vaccine is better than the effect of a subcutaneous injection immunization method under the same condition.

The present invention relates to a hydrogel, a preparation method thereof, and a pH sensor comprising the same, and more particularly, to a hydrogel, which can substitute for conventional pH paper, is continuously usable, can be used in various applications, and reversibly changes color depending on the pH of a sample, and to a pH sensor comprising the same. The hydrogel comprises: a polymer complex wherein a first organic compound containing an aromatic functional group having at least one hydroxyl group bonded thereto is joined to a carboxyl group-containing polysaccharide by a peptide bond; and an organic dye comprising a second organic compound containing an aromatic functional group having at least one hydroxyl group bonded thereto.

The invention describes a method for preparing a meningitis polysaccharide conjugate vaccine. The meningitis polysaccharide conjugate vaccine is developed based on the method. The method comprises the following steps: (1) activating meningococcus polysaccharides by cyanogen bromide, then deriving the meningococcus polysaccharides which are activated by the cyanogen bromide by ethanediamine, finally deriving the polysaccharides by a reagent which is of a structure of succinimidyl ester-R-maleimide; (2), sulfhydrylating carrier proteins; (3) combining the derived meningococcus polysaccharides with the sulfhydrylated carrier proteins. As conjugation bridges between the meningococcus polysaccharides and the carrier proteins are very long, the spatial shielding effect of the carrier proteins on the antigenic epitopes of the polysaccharides is reduced, and the original immunization property of the polysaccharide conjugate vaccine is improved.

The present invention provides a nucleotide molecule, which has a sequence represented by SEQ ID NO.:2, 4 or 6. The present invention further provides a recombinant protein PspA1, PspA2 or PspA3 encoded by the nucleotide molecule of the present application. The present invention further provides a polysaccharide conjugation vaccine, which comprises meningococcal polysaccharide and a carrier protein bound to the meningococcal polysaccharide, wherein the carrier protein is the recombinant protein PspA1, PspA2 or PspA3 encoded by the nucleotide molecule of the present application. The present invention further provides uses of the recombinant proteins PspA1, PspA2 and PspA3 encoded by the nucleotide molecule of the present invention as the carrier protein of the polysaccharide conjugation vaccine. The present invention further provides an expression vector, which comprises the nucleotide molecule of the present invention.

The invention discloses a single crystal silicon carbide green and efficient polishing tool. The tool comprises the following raw materials of, by weight, 48-75% of an abrasive material, 1-2% of a reinforcing phase, 0.35-0.75% of a dispersing agent and the balance polysaccharide binding agents. A method for preparing the single crystal silicon carbide green and efficient polishing tool with the materials above specifically comprises the following steps of material mixing, curing, polishing tool bonding and polishing tool shaping. The invention further discloses a method for polishing single crystal silicon carbide based on the single crystal silicon carbide green and efficient polishing tool prepared through the method. The tool is in contact with single crystal silicon carbide to generatedry friction, and the oxygen partial pressure range of the ambient atmosphere of a friction region ranges from 20 Pa-100 Pa. According to the technical scheme, the single crystal silicon carbide green and efficient polishing tool successfully prepared through the method is environment-friendly, non-toxic and low in manufacturing and polishing processing cost, and and green and efficient polishingon the surface of the single crystal silicon carbide can be realized.

The invention discloses an edible fungus polysaccharide mixed high-concentration protein beverage and a production method thereof. The edible fungus polysaccharide mixed high-concentration protein beverage comprises, by weight, 0.2-1% of edible fungus polysaccharide crude extract, 2-5% of whey protein isolate and/or soybean protein isolate and the balance of water, with pH of 3-6. The invention provides an edible fungus polysaccharide mixed high-concentration whey protein or soybean protein or whey-soybean protein beverage and a making method thereof. Edible fungus polysaccharides bind with proteins under certain conditions to form a soluble complex; high-concentration proteins are stably dispersed in a solution. The edible fungus polysaccharides can be mixed with a single-protein or dual-protein beverage to provide two nutrients, excellent proteins and polysaccharides; the manufacturing process has low equipment cost and low energy consumption; the edible fungus polysaccharide mixed high-concentration protein beverage is easy to popularize in edible fungus production areas, milk production areas, soybean production areas and other areas.

A kit for the determination of the ability of a peripheral blood mononuclear cell to bind to a polysaccharide herein provided comprises a detection reagent comprising a fluorescence-labeled immunostimulant polysaccharide; and a reference reagent comprising a fluorescence-labeled material of a polysaccharide different from that used in the fluorescence-labeled immunostimulant polysaccharide, and/or an immunostimulant polysaccharide which is not labeled with any fluorescent material and whose polysaccharide moiety is identical to that included in the fluorescence-labeled immunostimulant polysaccharide. The use of this kit would permit the estimation of the polysaccharide-binding ability of a peripheral blood mononuclear cell in a higher precision of estimation.

The invention discloses a kit for genome DNA extraction and an application of the kit. The kit comprises a lysis solution and an erythrocyte lysis solution, wherein the lysis solution contains 20 to 300mM of Tris-HCl, 20 to 100mM of EDTA, 0.3 to 2M of NaCl, 0.5 to 5 percent of PEG8000 and 0.5 to 10 percent of Triton X-100, and SDS or Tween 20; the erythrocyte lysis solution contains 10 to 100mM ofTris-HCl, 1 to 50mM of EDTA, 10 to 300mM of NaCl and 0.01 to 0.2 percent of Triton X-100. According to the kit, through optimization of the lysis solution and the erythrocyte lysis solution, polysaccharide can be effectively removed, phenol is combined, phenol is prevented from being combined with DNA, the problems of viscosity and turbidity are solved, the kit is suitable for various different samples, and the purity and yield of extracted genome DNA can meet various downstream experiment use requirements.

The invention relates to the technical field of vaccine conjugates, in particular to a preparation method of a group A meningococcus capsular polysaccharide conjugate, which comprises the steps of polysaccharide derivative preparation, mixing proportioning, conjugation reaction, aging treatment, ultrafiltration and sterilization. According to the method, the processes of pH adjustment in the activation process and conjugate purificationare omitted through optimization of all technological parameters, but the quality of the harvested polysaccharide conjugate still meets the national standard, so that the preparation process of the polysaccharide conjugate is simplified, and the preparation period of the polysaccharide conjugate is shortened.

A camptothecin derivative comprising a compound of the formula [I] is disclosed:wherein R1, R2, R3, R4 and R5 are (A) adjacent two groups combine to form alkylene, or both are H, and one of the remaining three groups is -Xn-Alkm-R6, and the other two are H, alkyl or halogen, or (B) adjacent two groups combine to form alkylene, and one of the carbon atoms of said alkylene group is substituted by -Xn-Alkm-R6, and the remaining three groups are H, alkyl or a halogen, and one or two -CH2- of the alkylene in (A) or (B) may optionally be replaced by -O-, -S- or -NH-, X is -O- or -NH-, Alk is alkylene, R6 is -NH2,or -OH, m and n are both 0 or 1, or m is 1 and n is 0, which camptothecin compound is bound to a polysaccharide having carboxyl groups via an amino acid or a peptide, or a pharmaceutically acceptable salt thereof. Said camptothecin derivatives show enhanced antitumor activities but few side effects and are useful as a medicament.

The invention discloses a method for determining a binding rate of a proteoglycan protein binding site in a proteoglycan protein conjugate vaccine. The method comprises the following steps of: (1) carrying out enzymolysis on a standard substance; (2) carrying out solid-phase extraction to enrich a target peptide fragment; (3) performing high performance liquid chromatography tandem mass spectrometry analysis; (4) drawing a standard curve to obtain a standard working curve equation; (5) detecting a sample, and calculating to obtain the concentration of each characteristic peptide fragment in the sample; and (6) calculating a proteoglycan binding rate of each characteristic peptide fragment and a protein residue rate of each site. According to the method, the multi-site quantitative monitoring of the proteoglycan protein binding rate of the proteoglycan protein conjugate vaccine is achieved, the tetanus protein relates to 20 monitoring sites, and the CRM197 relates to 16 monitoring sites; the method not only can detect the residual protein content, but further can evaluate the binding rate of the proteoglycan protein binding site of the proteoglycan protein conjugate vaccine, can beused for researching polysaccharide or proteoglycan protein conjugate vaccines with the same protein residual rate but different activity and toxicity, and can provide a direct monitoring method for improving a proteoglycan protein binding process.

The invention discloses dendrobium officinale polysaccharide and a preparation method thereof. The dendrobium officinale polysaccharide comprises dendrobium officinale roots, the dendrobium officinaleroots are subjected to a water extraction and alcohol precipitation method to extract crude polysaccharide, separation and purification methods such as repeated freezing and thawing, H2O2 decoloration, ultrafiltration, dialysis and the like are combined to respectively obtain two kinds of dendrobium officinale polysaccharide DOP-1 and DOP-2 with uniform molecular weight. Due to the fact that theviscosity of DOP-2 is lower than that of DOP-1, the 2D nuclear magnetic detection related peak response is good, DOP-1 and DOP-2 are smashed after multiple determination, then water at the temperatureof 220 DEG C is added for soaking and heating, heating is stopped when the DOP-1 and the DOP-2 are dispersed, residues in the DOP-1 and the DOP-2 are filtered, a concentrated solution is extracted, clear liquid floating on the concentrated solution is removed through a water extraction and alcohol precipitation method, and then the concentrated solution is precipitated and dried at high temperature to finally obtain the dendrobium officinale polysaccharide, so that the sugar content of the dendrobium officinale is increased, more raw materials are saved in the preparation process, and the reaction rate is effectively increased through hydroxyl free radicals and superoxide anions.

The present invention provides a nucleic acid-polysaccharide complex that is highly stable and has reduced cytotoxicity in a high yield. That is, the present invention provides a nucleic acid-polysaccharide complex of an antisense DNA having polydeoxyadenine (dA) as a polysaccharide binding site and a polysaccharide having a β-1,3-glucan skeleton wherein at least part of phosphodiester links of the polydeoxyadenine is phosphorothioated (provided that the phosphodiester links of the antisense DNA and the polydeoxyadenine are not entirely phosphorothioated).

The invention provides a preparation method of acid-soluble soybean protein-chitosan nanoparticles. The preparation method comprises the following steps: dissolving soybean protein isolate in water toobtain a solution A; dissolving chitosan in an acetic acid aqueous solution to obtain a solution B; mixing the solution A and the solution B, fixing the volume by using an acetic acid aqueous solution, adjusting the pH value to 3.0-3.55, and performing heating for 20-30 minutes by adopting high-pressure steam at 115-125 DEG C, so as to obtain an acid-soluble soybean protein mixed solution, namelythe acid-soluble soybean protein-chitosan nanoparticles. The acid-soluble soybean protein-chitosan nanoparticles are prepared by adding the polysaccharide and combining high-pressure heat treatment,and compared with a mode of only adding the polysaccharide and not combining high-pressure heat treatment, the light transmittance of the obtained acid-soluble soybean protein mixed solution is remarkably improved, the particle size of the acid-soluble soybean protein-chitosan nanoparticles is remarkably reduced, and the particle size of the acid-soluble soybean protein-chitosan nanoparticles is remarkably reduced. No additive or enzymic preparation is added in the preparation process, so that the production efficiency is high, the technological operation is simple, the cost is reduced, and the safety of the product is improved.

The invention discloses a method for determining the content of free polysaccharides in a conjugate vaccine. The method comprises the following steps: carrying out salting-out treatment on a conjugatevaccine stock solution; adding absolute ethyl alcohol into the stock solution subjected to salting-out treatment, and obtaining a mixed solution A; carrying out centrifugal treatment and separation on the mixed solution A to obtain a mixed precipitate of a polysaccharide protein conjugate and free sugar; S4, adding the mixed precipitate into water to obtain a mixed solution B; carrying out centrifugal treatment on the mixed solution B, and collecting supernatant as a test solution; and determining the polysaccharide content of the test solution, and recording the polysaccharide content as thefree polysaccharide content in the conjugate vaccine. According to the method for determining the content of free polysaccharide in the conjugate vaccine, polysaccharide binding protein in the conjugate vaccine is irreversibly denatured by adding ethanol to precipitate and separate the polysaccharide binding protein in the conjugate vaccine, thereby reducing influence of the polysaccharide binding protein in the conjugate vaccine on the determination of the free polysaccharide content and obtaining more accurate free polysaccharide content.