The invention provides a pharmaceutical composition ethosome, which is prepared from the following components in percentage by weight: 0.1 to 0.2 percent of thymosin beta4, 0.5 to 1 percent of glucocorticoid, 30 to 50 percent of low-molecular-weight alcohol, 3.0 to 12.0 percent of phospholipid, 1.0 to 2.5 percent of cholesterol, 0.51 to 1.3 percent of auxiliaries and the balance of PBS (Phosphate Buffer Solution). The invention also provides a method for preparing the pharmaceutical composition ethosome for treating scars. According to the ethosome, the dosage of hormone can be reduced, side effects can be reduced, the treatment effect is more lasting, scars can be shrunk in the medication process, and after medication is stopped, the scars are not prone to relapse and reach 1 + 1gt; and 2, efficacy. The ethosome disclosed by the invention is strong in transdermal ability, high in encapsulation efficiency, large in drug loading capacity, good in stability and easy to store and use. In addition, the ethosome provided by the invention has toxic and side effects, and is convenient, safe and controllable in administration, good in compliance and higher in bioavailability.