4 results about "Phosphoglucomutase" patented technology

The present application relates to the technical field of biotechnology, and particularly relates to a tagatose-6-phosphate phosphatase mutant and a method for synthesizing tagatose from scratch. The method provided by the present application is a one-pot method for biosynthesizing D-tagatose by using starch or dextrin as a substrate, adopting a multi-enzyme cascade reaction system composed of a tagatose-6-phosphate phosphatase mutant, a glycogen-debranching enzyme, an alpha-glucan phosphorylase, a phosphoglucomutase, a glucose-6-phosphate isomerase, a D-tagatose-6-phosphate-3-epimerase and a 4-alpha-glucan transferase. The method can prepare tagatose from ordinary and inexpensive starting materials (starch or dextrin) at a high yield, improves the overall conversion rate of the system and the final yield of tagatose, provides a new technical approach for realizing low-cost and green production of tagatose, and has the advantages of high yield, low cost and being more suitable for industrial production.

The invention relates to the technical field of biological catalysis, and provides a method for catalytic synthesis of trehalose-6-phosphoric acid, key parameters in a multi-enzyme cascade catalytic reaction system for catalytic synthesis of trehalose-6-phosphoric acid are adjusted, and in the catalytic system, the enzyme activity concentration of maltose phosphorylase is 80-120U / mL, the enzyme activity concentration of maltose phosphorylase is 20-30U / mL, and the enzyme activity concentration of maltose phosphorylase is 20-30U / mL. The enzyme activity concentration of the beta-glucophosphate mutase is 15-35 U / mL, the enzyme activity concentration of the trehalose-6-phosphate phosphorylase is 3-15 U / mL, and the yield of the trehalose-6-phosphate can be remarkably improved by adjusting a corresponding production process, so that an enzyme dosage combination with higher universality and high efficiency is obtained, and the method is suitable for industrial production. The conversion rate from the substrate to the target product trehalose-6-phosphoric acid can be up to 96%, the operation is simple and convenient, the cost is low, and the efficient and low-cost industrial production of the trehalose-6-phosphoric acid is realized.

This invention provides a hydrogen production reaction system using formaldehyde and water as co-substrates, comprising a buffer solution, maltodextrin, formaldehyde, magnesium chloride, manganese chloride, NAD, benzyl viologen, sodium phosphate, α-glucan phosphorylase, phosphogluconomutase, glucose-6-phosphate dehydrogenase, phosphogluconate dehydrogenase, phosphogluconolactonease, flavoxase, hydrogenase, 6-phosphate hexulose synthase, phosphogluconose isomerase, and phosphogluconose isomerase. This invention also provides a corresponding hydrogen production method. This invention utilizes formaldehyde and water to generate hydrogen, avoiding excessive dependence on agricultural resources; simultaneously, by replacing traditional carbohydrates with high-energy formaldehyde as the substrate, it lowers the energy barrier of the co-substrate water and simplifies the process of carbohydrate miniaturization and hydrogen production; furthermore, it has the advantages of being less demanding in terms of water quality conditions and having low cost.

The invention relates to a property-changed phosphoglucomutase mutant and application thereof, and belongs to the technical field of enzyme engineering. According to the invention, the phosphoglucomutase is mutated at different sites, the property of the obtained phosphoglucomutase mutant is changed, especially the thermal stability of the phosphoglucomutase mutant is obviously improved, the residual enzyme activity of the phosphoglucomutase mutant at 30 DEG C is also greatly improved at 65 DEG C, and the residual enzyme activity of the phosphoglucomutase mutant at 65 DEG C is also greatly improved. Therefore, the phosphoglucomutase mutant disclosed by the invention can still keep good enzyme activity in a biological process needing to be carried out at high temperature, and has wide application value in industrial application.