101 results about "Brucella" patented technology

The invention relates to the technical field of space-time prediction, in particular to a brucellosis space-time prediction method and system based on artificial intelligence, and the method comprises the following steps: obtaining case information, calculating an incidence relation, extracting a path sequence, screening a trend direction, and matching a new case to generate prediction data. According to the method, continuous identification of a propagation path is realized by constructing a propagation incidence relation between cases and introducing a spatial directivity index, a non-trend propagation process is screened out through an angle average value and a variance, spatial consistency of path screening is enhanced, and through joint matching of spatio-temporal characteristics of newly-added cases and an existing propagation trend, the propagation path screening efficiency is improved. The sensitivity of prediction to the trend attribution of a new case is improved, the spatial directivity of a potential disease area is enhanced through reverse projection of a trend path and area positioning drop point frequency analysis, and through linkage processing of multi-level path extraction, trend judgment and area coding, the probability of occurrence of the new case is lowered. And the capturing capability of the prediction data on the propagation and evolution characteristics of the brucellosis is improved.

The invention discloses a primer group, a kit and a method for detecting brucella, and belongs to the technical field of gene detection. The primer group comprises two pairs of specific RPA (recombinase polymerase amplification) primers: a primer pair B1F1 (SEQ ID No.3) and a primer pair B1R1 (SEQ ID No.6) targeting a B1 sequence, and a primer pair B2F1 (SEQ ID No.8) and a primer pair B2R1 (SEQ ID No.11) targeting a B2 sequence. The invention also provides a kit containing the primer group, and a detection method based on the RPA-CRISPR-Cas12a. The invention also provides a kit containing the primer group and a detection method based on the RPA-CRISPR-Cas12a. According to the application, the high efficiency of RPA amplification is combined with the ultrahigh specificity of CRISPR-Cas12a detection, the defects of long time consumption, low sensitivity and easy generation of false positive in the traditional method are overcome, rapid, sensitive and specific detection of Brucella is realized, and the application has important application value in early screening and monitoring of Brucella.

The invention relates to a silicon-based nano material for targeted therapy of brucellosis as well as a preparation method and application of the silicon-based nano material. The invention relates to a preparation method of a silicon-based nano material for targeted therapy of brucellosis. The preparation method comprises the following steps: (1) preparing magnetic mesoporous silica; (2) aminating the magnetic mesoporous silica, and modifying the magnetic mesoporous silica with a responsive material and yeast beta-glucan to obtain modified magnetic mesoporous silica; and (3) carrying out antibiotic loading on the modified magnetic mesoporous silica. According to the silicon-based nano material for targeted therapy of brucellosis as well as the preparation method and the application of the silicon-based nano material, the surface of magnetic macroporous mesoporous silica is modified with yeast beta-glucan capable of targeting M cells and glutathione responsive molecules, and a drug delivery system sensitive to a brucellosis environment is synthesized; the nano-carrier has targeted targeting selectivity for delivering and releasing drugs, the activity, slow release and target cell uptake efficiency of antibiotics are ensured, and the brucellosis treatment is more accurate and efficient.

The invention relates to the technical field of markers, in particular to application of a biomarker for identifying acute stage and chronic stage of brucellosis. A series of biomarkers related to acute and chronic stages of brucellosis are screened and found, and the biomarkers have obvious difference between acute and chronic brucellosis patients. The biomarkers can realize acute and chronic staging of brucellosis, are high in sensitivity and specificity, and have relatively good diagnosis efficiency. A novel molecular diagnosis tool is provided for acute and chronic staging of brucellosis, the problems that existing serological detection is low in specificity, and clinical staging cannot be accurately judged are solved, and wide application prospects are achieved.

This invention discloses an antigen and vaccine preparation based on dominant Brucella epitopes, and their application, belonging to the field of recombinant subunit vaccine technology. The antigen is composed of CTL epitopes, HTL epitopes, and B-cell epitopes tandemly, and its amino acid sequence is shown in SEQ ID NO.1. Based on screened dominant CTL epitopes, HTL epitopes, and B-cell epitopes of the Omp25 and Omp31 outer membrane proteins, this invention constructs a novel antigen fusion polypeptide. The vaccine prepared based on this polypeptide effectively activates a significant dual immune response, enhancing cellular immunity while inducing the body to produce high levels of specific antibodies. It also provides good protection against organ damage caused by bacterial infection, offering better immune response and protective efficacy against Brucella, laying the foundation for the development of Brucella recombinant subunit vaccines.

Provided herein are methods and compositions for the detection of Brucella antigen, and more particularly, to a vaccine or immunogenic composition comprising: Brucella strain from which at least a portion of the immunogenic protein DUF883 has been deleted provided in an amount sufficient to trigger an immune response against the immunogenic protein; and a pharmaceutically acceptable vaccine carrier.

The application discloses a serological method for identifying Brucella A19-DeltaVirB12 vaccine immunized animals and infected animals, and belongs to the technical field of veterinary vaccine detection, comprising the step of identifying the antibodies of the serum by using the NH-AGID method. The method helps to perfect the animal Brucella A19-DeltaVirB12 vaccine prevention and control technical system, makes up for the short board that the A19-DeltaVirB12 vaccine immunized animals cannot be identified, and provides technical support for realizing the precise prevention and control of bovine brucellosis. The application of the method will accelerate the application of the Brucella A19-DeltaVirB12 vaccine to replace the traditional A19 vaccine, and opens a new milestone for the prevention and control of Brucella.

In one aspect, an EID button tag is provided herein for tagging and identifying cattle as being inoculated against brucellosis, the EID button tag including: a shell having a central opening; a transponder located in the shell; a male punch having a disc-shaped punch body and a stem extending distally therefrom, the stem having a head configured for irreversible insertion into the central opening, wherein a mounting opening is formed through the punch body with a mounting channel extending distally from the mounting opening and coaxially along the stem; and, a vaccination identification button having a disc-shaped visual identification body and a mounting stem extending distally therefrom, wherein the mounting stem is configured for irreversible insertion into the mounting channel. The punch body and the visual identification body are primarily tinted with different colors to provide visual identification of a head of cattle being inoculated against brucellosis.

This invention provides a live attenuated Brucella vaccine strain M5Δ pyrE Its construction methods and applications belong to the field of biotechnology. The M5... pyrE The strain was obtained by knocking out Brucella mesenteriae strain M5. pyrE Genetically constructed and deposited at the China Center for Type Culture Collection. This invention discovers that... pyrE The gene is associated with Brucella virulence; knocking out this gene significantly weakens Brucella virulence and reduces its intracellular and mouse viability. Compared to the existing vaccine strain M5-9026, M5... pyrE The strain exhibits superior immunoprotective effects, effectively inducing humoral and Th1 cellular immune responses, and demonstrates good safety. The M5 strain constructed in this invention... pyrE This strain has promising potential as a candidate strain for a live attenuated vaccine to prevent brucellosis in animals.

The application relates to a Brucella typing detection kit and a detection method thereof, and relates to the technical field of biological detection, and comprises the following components: a reaction solution, a detection solution, an enzyme mixed solution, a quantitative standard, a typing standard and a blank control; the reaction solution comprises 5xPCR buffer, 50mM MgCl2, 0.5Mm dNTP, 0.1-0.3% (v / v) surfactant, 0.5-2mol / L enhancer, 1-5% (w / v) trehalose, 0.1-1% (w / v) BSA, 0.1-1ug / muL SSB and 0.1-1mM MnCl2. The kit and the detection method thereof are excellent in signal strength, complex sample tolerance, detection sensitivity and specificity.

The invention relates to the field of biological detection, in particular to a primer probe set, a detection reagent and application of the primer probe set and the detection reagent in detection of virulent pathogenic bacteria. The invention provides a primer probe group. The primer probe group comprises a primer probe group used for amplifying bacillus anthracis, Francisella tularensis, bacillus plague, rhinotrichum-like bacteria, brucella, vibrio cholerae, salmonella typhimurium and staphylococcus aureus, wherein the primer probe group is used for amplifying bacillus anthracis, Francisella tularensis, bacillus plague, rhinotrichum-like bacteria, brucella, vibrio cholerae and salmonella typhimurium. The invention establishes a primer probe combination for rapidly and sensitively detecting various virulent pathogenic bacteria and a kit thereof, aiming at eight virulent pathogenic bacteria such as bacillus anthracis, Francisella tularensis, bacillus plague, rhinotrichum, vibrio cholerae, brucella, salmonella typhimurium and staphylococcus aureus. The kit is stored in a freeze-dried state, adaptive sample lysate can directly expand different types of samples, and the kit has the advantages of being good in specificity, high in sensitivity, short in detection time, good in accuracy, good in stability after redissolution and good in compatibility.

The application belongs to the technical field of biological pharmacy, and particularly relates to a bovine-derived mouse anti-Brucella antibody and a preparation method and application thereof. A bovine-derived mouse anti-Brucella antibody is prepared by synthesizing a tandem epitope protein of Brucella OMP25 proteins of bovine species, sheep testis species and canine species, immunizing mice, preparing a monoclonal antibody, cloning CDR fragments in Hv and Lv regions of the antibody to corresponding positions of bovine-derived IgG, and then performing overall mutation modification on the recombinant IgG to perform bovine-derived modification. The bovine-derived mouse anti-Brucella antibody preparation has good immunogenicity and protection effect, and can block and inhibit the propagation and spread of Brucella of bovine species, canine species and sheep testis species in an animal body.

This invention provides a primer-probe combination, reagents, methods, and applications for detecting Brucella vaccine strain RB51, belonging to the field of pathogen detection technology. The primer-probe combination for detecting Brucella vaccine strain RB51 of this invention includes a forward primer RB51-F, a reverse primer RB51-R, and a probe RB51-P; the forward primer RB51-F is shown in SEQ ID NO:1, the reverse primer RB51-R is shown in SEQ ID NO:2, and the probe RB51-P is shown in SEQ ID NO:3. The fluorescent PCR and digital PCR detection methods established based on the primer-probe combination of this invention have high sensitivity, good specificity, fast detection speed, and high throughput, enabling rapid detection of Brucella vaccine strain RB51, making differential diagnosis more accurate, economical, efficient, and convenient.

The invention relates to a Brucella lipopolysaccharide monoclonal antibody and a cELISA detection kit and detection method based on the same, the monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and is characterized in that the amino acid sequence of the heavy chain variable region is SEQ ID No.1, and the corresponding nucleotide sequence is SEQ ID No.2; the amino acid sequence of the light chain variable region is SEQ ID No.3, and the corresponding nucleotide sequence is SEQ ID No.4. The monoclonal antibody is only specifically combined with smooth LPS of brucella, and has no cross reaction with 21 pathogens such as rough brucella, yersinia O: 9 and the like. The sensitivity of a competitive enzyme-linked immunosorbent assay (cELISA) kit developed on the basis of the antibody reaches 1: 25600 and is improved by 200 times compared with that of a traditional method, the detection time is shortened to 90 min, the in-plate / inter-plate variable coefficient is lower than 9.87%, and the antibody is suitable for high-throughput screening of multi-host serum.

The application discloses a set of monoclonal antibodies for Brucella antigen sandwich method detection and application thereof, and relates to the technical field of biotechnology, and provides a set of monoclonal antibodies for Brucella antigen sandwich method detection, which is composed of 3G6 antibodies, 4H5 antibodies, 2A11 antibodies and 4B11 antibodies; each antibody comprises a heavy chain variable region and a light chain variable region. The set of monoclonal antibodies has the beneficial effects that the set of monoclonal antibodies can realize specific antigen detection of ovine Brucella and Brucella through different antibody combinations, and can reduce false positives in detection. The set of monoclonal antibodies can be used for detection of various samples such as foodborne food and environment, and can also be used for identification of pure culture ovine Brucella and Brucella.

This application discloses a composition, a method for preparing the composition, and its application, comprising: an mRNA molecule containing a first sequence; and an LNP nanoparticle delivery carrier; wherein the protein encoded by the first sequence has BP26 protein immunogenicity, the first sequence encodes a first amino acid sequence, the first amino acid sequence includes multiple sub-sequences in tandem, the multiple sub-sequences being selected from the amino acid sequences corresponding to T cell and B cell recognition epitopes in wild-type BP26 protein, and the composition has high immunogenicity and high targeting against Brucella.

This invention discloses a primer set, kit, and method for detecting Brucella, belonging to the field of gene detection technology. The primer set comprises two pairs of specific RPA primers: primer pairs B1F1 (SEQ ID No. 3) and B1R1 (SEQ ID No. 6) targeting the B1 sequence, and primer pairs B2F1 (SEQ ID No. 8) and B2R1 (SEQ ID No. 11) targeting the B2 sequence. This invention also provides a kit containing this primer set and a detection method based on RPA-CRISPR-Cas12a. This application combines the high efficiency of RPA amplification with the ultra-high specificity of CRISPR-Cas12a detection, overcoming the shortcomings of traditional methods such as long processing time, low sensitivity, and susceptibility to false positives. It achieves rapid, sensitive, and specific detection of Brucella, and has significant application value in the early screening and monitoring of brucellosis.

The invention belongs to the technical field of genetic engineering and diagnostic reagents, and particularly relates to a brucella recombinase polymerase amplification (RPA) detection kit and application, and the detection kit comprises a specific primer group and a probe. According to the invention, the RPA amplification product is detected by using the test strip, after amplification is carried out for about 10 minutes, the RPA amplification product is dropwise added into the test strip to read a result, and a PCR (Polymerase Chain Reaction) amplification instrument and a quantitative PCR instrument are not needed. The obtained method has the advantages of strong specificity, good sensitivity, simple operation process, rapid detection and the like, provides a possibility for the detection of brucellosis in the field, and also provides a method for the research of brucellosis which does not aim at the diagnosis and treatment of diseases.

The invention discloses a method for preparing a sheep-derived positive serum reference of yersinia enterocolitica, obtained positive serum and application of the positive serum, and belongs to the field of veterinary medicine products. The invention aims to solve the technical problem of how to obtain the yersinia enterocolitica sheep-derived positive serum reference which is stable in titer and character and can be used for specific detection of brucella diagnostic products. Therefore, the invention provides a method for preparing the yersinia enterocolitica sheep-derived positive serum reference, which comprises the following steps: immunizing a tested sheep by using an immunizing antigen, and collecting serum of the immunized tested sheep to obtain the yersinia enterocolitica sheep-derived positive serum reference. The immune antigen is an inactivated yersinia enterocolitica obtained by inactivating the yersinia enterocolitica. The positive serum reference can be effectively applied to quality control evaluation work of brucellosis related diagnosis products, and plays a positive role in prevention and control of brucellosis.

The invention discloses a Brucella bacterium capable of generating a lipoid A vaccine adjuvant and an application of the Brucella bacterium. According to the invention, recombinant plasmids for expressing BacA and LpxL genes are constructed, and are transformed into Brucella through electric shock, so that a double-gene knockout strain is successfully obtained. By knocking out BacA, an ultra-long fatty acid chain of lipoid A is shortened, an organism is induced to generate an inflammatory reaction, and by knocking out LpxL, the inflammatory reaction is reduced, so that the effect of the immunologic adjuvant is achieved. LPS is extracted for in-vitro stimulation analysis, and it is found that the inflammatory response of double-gene deletion is reduced compared with that of single-gene deletion. Animal experiments show that double-gene deletion can induce an organism to generate good immune response and induce relatively high cellular immunity and humoral immunity, and a new thought is provided for research and development of gram-negative bacterium inactivated vaccines.

Provided herein are methods, kits and systems for the detection of Brucella antigen, method for detecting brucellosis in a mammal comprising the steps of: obtaining or having obtained a biological sample suspected being infected with Brucella sp.; and detecting in the biological sample immunogenic protein DUF883 or anti-DUF883 antibodies, wherein the presence of DUF883 or anti-DUF883 antibodies are indicative that the mammal has been exposed to Brucella, e.g., to a vaccine or immunogenic composition comprising: immunogenic protein DUF883 provided in an amount sufficient to trigger an immune response against the immunogenic protein; and a pharmaceutically acceptable vaccine carrier.

The application discloses an ELISA antibody detection kit for identifying Brucella gene deletion live vaccine (M5-90△26 strain) and wild strains and application. The kit comprises enzyme-linked reaction plate 1 and enzyme-linked reaction plate 2. The enzyme-linked reaction plate 1 is coated with LPS extracted from the Brucella gene deletion live vaccine (M5-90△26 strain), and the enzyme-linked reaction plate 2 is coated with a Brucella BP26 antigen epitope polypeptide combination composed of polypeptides with amino acid sequences shown in SEQ ID NO:1-2. The application is suitable for goats and sheep inoculated with the Brucella gene deletion live vaccine (M5-90△26 strain), can effectively distinguish the antibody produced by the Brucella gene deletion live vaccine (M5-90△26 strain) and the antibody produced by wild strain infection, thereby realizing identification and detection, being favorable for prevention and control of brucellosis and optimization of a vaccination strategy, and providing technical support for healthy and sustainable development of animal husbandry.

The invention relates to the technical field of biological detection, and particularly discloses a preparation method and application of a Brucella antibody immunochromatography detection test strip based on colloidal carbon. The Brucella antibody immunochromatography detection test strip provided by the invention comprises a PVC (Polyvinyl Chloride) bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad are arranged on the PVC bottom plate; the binding pad contains lipopolysaccharide of colloidal carbon labeled brucella, the nitrocellulose membrane is provided with brucella lipopolysaccharide and a goat anti-mouse igG antibody which are respectively used as a detection line (T) and a quality control line (C), and the colloidal carbon technology-based immunochromatography test paper is established by detecting different specimens and comparing with other detection methods. The test strip prepared by the invention has the advantages of no subjective influence of a detector, simplicity and convenience in operation, high detection sensitivity, strong specificity and the like.

The invention relates to the technical field of gene detection, in particular to an omega RNA (Ribonucleic Acid) for detecting brucella, a fluorescence detection kit, a visual kit and application thereof. The omega RNA nucleotide sequence provided by the invention is as shown in SEQ ID NO: 4. According to the present invention, the provided omega RNA can specifically recognize the Brucella bp26 gene, the amplification product of the Brucella bp26 gene combined with the TnpB protein has high side cutting activity, the TnpB cuts the non-specific ssDNA report probe after the omega RNA recognizes the target sequence, the Brucella can be detected through the fluorescence detection or the nucleic acid detection test strip, and the high accuracy and the high sensitivity are provided; the Brucella can be detected at any time on a non-laboratory site through an OMEGA-TnpB detection system, and the method has the advantages of being wide in universality, low in operation requirement, efficient and accurate.

The invention provides a specific polypeptide of Brucella, the amino acid sequence of the specific polypeptide is as shown in SEQ ID No.1, and the specific polypeptide is derived from BMEI1871 protein of Brucella. The invention also provides a recombinant vector or an expression cassette for expressing the specific polypeptide, and a recombinant cell or a recombinant bacterium. The invention verifies the application of the specific polypeptide in preparation of drugs or reagents for preventing, detecting or diagnosing brucellosis, and also provides an ELISA (enzyme-linked immuno sorbent assay) detection kit for detecting brucellosis. According to the present invention, the specific polypeptide of the brucella BMEI1871 protein is adopted as the antigen to establish the ELISA method for detecting the brucella antibody, such that the accuracy is high, and the powerful support is provided for brucellosis purification. The antigen source is convenient, the stability is high, the repeatability is strong, and the defects of using whole bacteria or lipopolysaccharide as the antigen are overcome.

The invention belongs to the technical field of biological medicines, and particularly relates to a lactic acid bacteria live vector vaccine for preventing and treating brucellosis and a preparation method of the lactic acid bacteria live vector vaccine. The lactic acid bacteria live vector vaccine is obtained by transferring expression vectors for expressing Brucella omp19 protein and BvrR protein into lactic acid bacteria, and the lactic acid bacteria are lactobacillus cavings. The lactobacillus live vector vaccine for preventing and treating brucellosis is constructed by taking brucella omp19 protein and BvrR protein as immunogens and lactic acid bacteria as an antigen transfer vector, and the safety is good. After the vaccine is used for immunizing mice, the mice can obtain a higher antibody level, the duration is long, brucella infection is effectively inhibited, meanwhile, the production process of the vaccine is simplified, and the vaccine has a good application prospect.

The present application relates to the field of animal breeding technology, and particularly relates to a molecular marker related to sheep brucellosis susceptibility and application thereof.The molecular marker comprises nucleic acid of a nucleotide sequence as shown in SEQ ID NO.1, and polymorphism exists at the 101st position, and the polymorphism is G / A.A molecular marker related to sheep brucellosis susceptibility is screened by the present application, and the susceptibility of sheep brucellosis can be identified by detecting the polymorphism of the molecular marker.The molecular marker provided by the present application can also be used for breeding sheep varieties with low brucellosis susceptibility, which has important significance in the field of sheep breeding.

The present application relates to the field of animal breeding technology, and particularly relates to a molecular marker and application thereof in identifying sheep brucellosis susceptibility. The molecular marker comprises nucleic acid of a nucleotide sequence shown as SEQ ID NO. 1, and a polymorphism exists at the 101th position, and the polymorphism is C / G. The present application identifies a molecular marker related to sheep brucellosis susceptibility. By analyzing the genetic polymorphism of the marker, the susceptibility of sheep individuals to brucellosis can be determined. In addition, the molecular marker can also be used as an effective auxiliary selection tool, and is applied to breeding of new sheep strains with stronger brucellosis resistance, which has important application value for disease-resistant breeding of sheep.

The invention provides modified brucella as well as a preparation method and application thereof and a vaccine, and relates to the technical field of biology. The Omp25 gene of the modified brucella is replaced by an IFN (interferon)-gamma gene. The vaccine containing the modified Brucella is short in bacterium discharging time when being used for immunizing animals, the cellular immune effect can be improved, and prevention and control of Brucella are facilitated.

The invention discloses an integrated device and method for rapid separation and culture of brucellosis, and relates to the technical field of germ separation and culture. The Brucella rapid separation and culture integrated device comprises a bottom seat, the gas input pipeline is arranged in the bottom seat and extends to the outer side; the culture base is mounted at the top of the bottom seat; the germ culture medium is mounted at the top of the culture base; the sealing cover body is installed at the top of the culture base, a constant-temperature control structure extending into the bottom base is installed in the culture base, and the constant-temperature control structure comprises a tooth telescopic assembly installed in the center of the interior of the bottom base. According to the Brucella culture device disclosed by the invention, by arranging the recyclable gas capable of respectively carrying out temperature control treatment on the outer side and the inner side of the Brucella, the contact area of the Brucella and the temperature control gas is increased, and the culture effect of the Brucella is ensured. Meanwhile, the problem of accumulation of temperature control gas in a transmission state is avoided, and the brucellosis can be continuously cultured for multiple days.