121 results about "Colloidal gold test" patented technology

The invention relates to the technical field of molecular biology, in particular to an avian adenovirus serum type 4 colloidal gold test strip based on a monoclonal antibody and application of the test strip. According to the invention, two monoclonal antibodies (6B3 and 8G11) aiming at FAdV4 penton protein are successfully prepared through a hybridoma technology, and both the monoclonal antibodies show high titer and strong serotype specificity. Specific analysis proves that the two monoclonal antibodies can recognize 12 FAdV4 isolates from different sources, and do not have cross reaction with other FAdV serotypes or common poultry pathogens. According to the invention, through systematic optimization of key parameters, the detection sensitivity of the test strip is 7.81 * 10 < 4 > TCID50 / 0.1 mL, and the detection time is only 15 minutes. Clinical sample verification shows that compared with an ELISA method, the total coincidence rate is 98.6%.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for detecting candida albicans enolase protein and application. And the monoclonal antibody combination comprises monoclonal antibodies 5G7 and 5B1. The amino acid sequences of CDR regions of the monoclonal antibody 5G7 and the monoclonal antibody 5B1 are as shown in SEQ ID NO.1-12. The antibody combination has high specificity and binding activity, and can be used for efficient detection of candida albicans enolase. The colloidal gold test strip constructed on the basis of the combination has good sensitivity and specificity, the lowest detection limit can reach 500pg / ml, and the colloidal gold test strip does not have cross reaction with other common fungi. The invention provides a reliable tool for early diagnosis and rapid detection of invasive candida infection, and has a good clinical application prospect.

The invention discloses a monoclonal antibody and application thereof in quantitative detection of porcine epidemic diarrhea virus, and belongs to the technical field of biological detection. The preparation method comprises the following steps: firstly, obtaining two monoclonal antibodies GC44F3-1 and GC32C11-5 which specifically react with S protein of the porcine epidemic diarrhea virus; secondly, establishing a double-antibody sandwich ELISA (Enzyme-Linked Immunosorbent Assay) method by taking the two monoclonal antibodies as a coating antibody and a detection antibody respectively, and applying the double-antibody sandwich ELISA method to PEDV (Porcine Epidemic Diarrhea Virus) vaccine S protein quantification; the method can be used for detecting semi-finished products and finished products, can be used for process establishment, optimization, vaccine quality evaluation and stability monitoring of porcine epidemic diarrhea virus vaccine antigens, has the advantages of high sensitivity, high accuracy, high repeatability and high flux, is high in detection flux and high in speed, solves the problem that TCID50 cannot meet vaccine quality evaluation at present, and has a good application prospect. The defect that the existing S protein ELISA method can only be used for qualitative judgment is overcome. The monoclonal antibody can also be used for blocking ELISA, capturing ELISA, indirect ELISA, competitive ELISA and a reagent for detecting the porcine epidemic diarrhea virus through a colloidal gold test strip.

The invention belongs to the technical field of molecular diagnosis, and particularly discloses a high-risk HPV16 rapid detection system based on RPA-CRISPR / Cas12a, which is prepared by the following steps: (1) mixing a nucleic acid lysis solution containing Tris (pH = 8.0), NaHCO3, polyvinylpyrrolidone and Tween-20 with Proteinase K to treat a sample, and performing pyrolysis at room temperature for 5 minutes to obtain a nucleic acid template; (2) an RPA reaction system contains an RNA inhibitor, a reverse transcriptase and an RPA reaction essential enzyme, and after the template and the starting agent in the step (1) are added, RPA amplification is carried out at 37 DEG C; (3) dropping the reaction liquid on a colloidal gold test strip subjected to position exchange and transformation of a sample pad and a water absorption pad, and carrying out result interpretation; and (4) adding the RPA product into a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) reaction system containing the LbCas12a protein, the specific gRNA and the probe. According to the scheme, a rapid, high-sensitivity and cross-reaction-free detection system of the HPV16 is realized by optimizing a nucleic acid lysis solution formula, designing a specific primer and gRNA and modifying a test strip for detection.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody pair for detecting cat immunodeficiency virus p24 protein and application of the monoclonal antibody pair. The monoclonal antibody pair is a monoclonal antibody 1D8 and a monoclonal antibody 1H10 and can specifically recognize cat immunodeficiency virus p24 protein, and sequences of complementary determining regions of variable regions of a heavy chain and a light chain of the 1D8 and the 1H10 are clear and are respectively shown as SEQ ID NO.1-12. The antibody pair can effectively avoid cross reaction, and the accuracy and sensitivity of detection are remarkably improved; the colloidal gold test strip constructed on the basis of the antibody pair is simple, convenient and rapid to operate, is suitable for early antigen detection of FIV infection, solves the problem that natural infection and vaccine immunity cannot be distinguished in existing antibody detection, and provides a reliable technical means for FIV field screening and disease prevention and control.

The invention discloses a T-2 toxin monoclonal antibody and application thereof, and belongs to the technical field of biology. The monoclonal antibody contains a heavy chain variable region and a light chain variable region, the heavy chain variable region and the light chain variable region are both composed of complementary determining regions and frame regions, and the complementary determining regions are both composed of CDR1, CDR2 and CDR3. The monoclonal antibody can be used for preparing a T-2 toxin detection product. The T-2 toxin colloidal gold test strip provided by the invention has the characteristics of high sensitivity, good specificity and strong stability.

The invention provides a colloidal gold detection method and system based on computer vision, and relates to the technical field of colloidal gold detection.The colloidal gold detection method comprises the steps that a colloidal gold test paper strip image of a to-be-detected sample is collected in real time, the colloidal gold test paper strip image is preprocessed, and a target colloidal gold test paper strip image is obtained; positioning a detection line area and a quality control line area from the target colloidal gold test paper strip image, respectively extracting image data in the detection line area and the quality control line area, constructing a multi-dimensional feature data set, and inputting the multi-dimensional feature data set into a preset colloidal gold concentration detection model to obtain concentration data of the to-be-detected sample, the problem that the accuracy and reliability of existing colloidal gold detection cannot meet the actual application requirements of high-precision quantitative detection is solved, and the effect of improving the accuracy of colloidal gold detection results is achieved.

The invention discloses a porcine epidemic diarrhea virus S protein monoclonal antibody and application thereof. Specifically, two monoclonal antibodies GC42G2-1 and GC33D8-1 which have broad-spectrum binding characteristics with the porcine epidemic diarrhea virus S protein are obtained firstly, the GC42G2-1 antibody has good universality and can have strong affinity with different strains, and the GC33D8-1 antibody can be used for binding the porcine epidemic diarrhea virus S protein in a broad-spectrum manner. The GC42G2-1 can be independently used, and also can be matched with a polyclonal antibody or GC33D8-1 to be used for sandwich ELISA (enzyme-linked immunosorbent assay) so as to detect and quantify PEDV (porcine epidemic diarrhea virus). Secondly, the two monoclonal antibodies are respectively used as a coating antibody and a detection antibody, a double-antibody sandwich ELISA method is established, and the double-antibody sandwich ELISA method is applied to PEDV vaccine S protein quantification, has better parallelism compared with other antibodies, and can be widely applied to detection of different strains. The method disclosed by the invention has the advantages of high sensitivity, high accuracy, high repeatability and high flux, and can be used for the development of blocking ELISA (Enzyme-Linked Immunosorbent Assay), capturing ELISA, indirect ELISA, competitive ELISA and detection of porcine epidemic diarrhea viruses by colloidal gold test strips.

The invention discloses an anti-rabies virus N protein monoclonal antibody, a detection reagent and application of the anti-rabies virus N protein monoclonal antibody. The monoclonal antibody for resisting the rabies virus N protein comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No. 1; the amino acid sequence of the light chain variable region is as shown in SEQ ID No.2. The monoclonal antibody with relatively high affinity and detection sensitivity for resisting rabies virus N protein lays a foundation for research, development and popularization of colloidal gold test strips and fluorescence immunoassay test strips.

The invention relates to a rapid detection method for nucleic acid of iridovirus of Micropterus salmoides and a kit thereof, belonging to the technical field of molecular biology, the rapid detection method adopts a two-step method of RPA-Cas12a for treatment, can be used for fluorescence detection through fluorescence labeling and also can be used for detection through an immune colloidal gold test strip, and is simple and convenient to operate, rapid and sensitive, strong in specificity, good in stability and low in cost.

The invention discloses a deep learning-based colloidal gold test paper result analysis system for detecting renal injury molecular 1. The system comprises an image acquisition module, a region processing module, an effectiveness judgment module, a concentration analysis module and a result generation module, the image acquisition module is used for acquiring a visible light image of the kim-1 colloidal gold test paper, wherein the visible light image at least comprises a detection line area and a quality control line area, and carrying out gray standardization processing, contrast enhancement processing, edge sharpening processing and target area positioning processing on the visible light image; the region processing module is used for performing positioning and boundary segmentation to obtain a mask image which is consistent with the input image in size and is marked with the position of each functional region; the effectiveness judgment module is used for judging whether the kim-1 colloidal gold test paper is effective or not; the concentration analysis module is used for predicting the target substance concentration of the kim-1 colloidal gold test paper; and the result generation module is used for generating an analysis result of the kim-1 colloidal gold test paper and packaging the analysis result into a structured detection report.

The application provides a preparation method of a colloidal gold solution, a preparation method of a colloidal gold labeled protein, a colloidal gold solution and a colloidal gold labeled protein prepared by the method, a kit comprising the colloidal gold solution or the colloidal gold protein, a labeling pad for a colloidal gold test strip, a colloidal gold test strip, and use of the colloidal gold solution, the colloidal gold labeled protein, the labeling pad or the colloidal gold test strip in preparing a kit for detecting a disease marker in a biological sample. For example, the kit of the application can be used for detecting C-reactive protein by a colloidal gold method.

The present disclosure discloses a universal aptamer-based colloidal gold lateral flow test strip for detecting small-molecule substances and belongs to the fields of analytical chemistry, medicine, environment, food safety detection, nano-biosensing and the like. An AuNPs@poly-DNA probe is used for rapidly and sensitively capturing an aptamer, streptavidin sprayed in a test zone and streptavidin-biotin-DNAc sprayed in a control zone do not need to be changed, and another substance can be detected only by changing a nucleic acid chain part of the AuNPs@poly-DNA probe. A universal colloidal gold lateral flow test strip, which is rapid, sensitive and low in cost, has been developed. The test strip method for detecting small-molecule substances is simple, convenient and rapid, and can be used for detection at any time. Only a test solution is needed to be added into a sample port and the test strip is completely developed after 5 min, thus an experiment result can be observed and the detection efficiency can be greatly improved. Qualitative analysis can be conducted by naked eyes, and quantitative analysis can be conducted by a colloidal gold test strip quantitative analyzer.

The invention discloses a feline leukemia virus p27 protein monoclonal antibody, a hybridoma cell strain and application thereof, and relates to the technical field of biology, the amino acid sequence of a heavy chain variable region of the monoclonal antibody is as shown in SEQ ID NO.1 in a sequence table, and the amino acid sequence of a light chain variable region of the monoclonal antibody is as shown in SEQ ID NO.2 in the sequence table. According to the invention, a cell fusion technology is utilized to establish a hybridoma cell strain p27-3A5 capable of secreting a p27 protein monoclonal antibody, and a specific monoclonal antibody 3A5 is obtained. And the minimum linear epitope identified by the antibody is identified, so that the establishment of subsequent preparation of a diagnostic kit such as a colloidal gold test strip is facilitated.

The utility model provides a colloidal gold kit clamping shell and colloidal gold kit with the same, the colloidal gold kit clamping shell comprises an upper clamping shell and a lower clamping shell which are buckled with each other, a sample adding hole and an observation window are formed on the upper clamping shell, a clamping groove for positioning a colloidal gold test strip is formed on the inner side surface of the lower clamping shell, and the upper clamping shell and the lower clamping shell are buckled with each other. The inner side surface of the upper clamping shell can limit the colloidal gold test strip in the clamping groove, the clamping groove is provided with a first groove bottom wall corresponding to the position of the sample adding hole, and the first groove bottom wall is closer to the upper clamping shell along the direction from the sample adding hole to the observation window to form a flow guide slope surface; the gradient of the diversion slope is a, and a is larger than or equal to 5 degrees and smaller than or equal to 10 degrees. According to the utility model, the conditions in the prior art that the chromatography is too fast and the detection line or the quality control line is pasty due to the fact that the sample is too thick and still runs on the plate or the sample is too thin and liquid rushes onto the test paper are effectively avoided.

The application discloses a colloidal gold test strip for distinguishing between wild toxin infection and vaccine immunization of porcine pleuropneumonia actinobacillus and application. The test strip of the application takes ApxIVA truncated protein with an amino acid sequence shown as SEQ ID NO. 3 as an antigen, the truncated protein can be soluble expressed in E. coli, and the structure and function of the truncated protein are close to those of natural apxIVA. The test strip of the application comprises a sample pad, a colloidal gold pad, an NC membrane, a water absorption filter paper and a bottom plate, the colloidal gold pad is coated with gold-labeled apxIVA truncated protein and gold-labeled chicken IgY antibody, and the C line and the T line of the NC membrane are fixed with goat anti-chicken IgY antibody and apxIVA truncated protein. The test strip only needs 25 muL of pig serum to make detection within 5-10 min, and can distinguish between APP wild strain infection and gene deletion vaccine APP-HB-04M immunized serum samples.

The present application relates to the technical field of biological medicine detection, and discloses a colloidal gold test strip for detecting anti-PLA2R antibody, a preparation method, a kit and application thereof.Capture antigens and detection antigens of the colloidal gold test strip are both PLA2R proteins, the nucleotide sequence of the PLA2R protein is shown as SEQ ID No.1, and the amino acid sequence of the PLA2R protein is shown as SEQ ID No.2.The colloidal gold test strip for detecting anti-PLA2R antibody and the kit have the characteristics of simplicity, good stability, high sensitivity, good specificity, simple operation, suitability for on-site rapid detection, rapid result output and directness, and can be applied to family and bedside detection without special laboratory and experimental personnel.

The invention belongs to the technical field of virus immunodetection, and relates to detection of swine transmissible gastroenteritis virus, in particular to a Mab 12H6 antibody, a colloidal gold test strip and application. According to the invention, TGEV N protein is used as an antigen of a detection line, Mab 12H6 is used as a detection antibody combined with colloidal gold, condition optimization is carried out through different methods, the specificity and sensitivity of the methods are evaluated, and a detection method for detecting the antigen based on an N protein TGEV colloidal gold competition method is established. The established TGEV colloidal gold test strip has the advantages of strong specificity, high sensitivity, simplicity and convenience in operation, low production cost and the like, and can be applied to rapid detection of TGEV in a porcine diarrhea sample clinically.

The invention relates to the technical field of veterinary biology, in particular to a monoclonal cell strain related to mycoplasma synoviae, a test strip and a preparation method and application of the monoclonal cell strain and the test strip. A heavy chain VH nucleotide sequence of a chicken mycoplasma synoviae antibody secreted by the monoclonal cell strain MS1C5-F8 is SEQ ID NO.1, an amino acid sequence of the chicken mycoplasma synoviae antibody is SEQ ID NO.3, a light chain VL nucleotide sequence of the chicken mycoplasma synoviae antibody is SEQ ID NO.2, and an amino acid sequence of the chicken mycoplasma synoviae antibody is SEQ ID NO.4. The chicken mycoplasma synoviae antibody not only can be subjected to specific reaction with different MS clinical isolate antigens, but also has no cross reaction with other common avian bacterial pathogens. The monoclonal antibody cell strain is used for immunizing a mouse to prepare ascites and purifying a corresponding antibody, and the purified monoclonal antibody is used for successfully preparing a colloidal gold test strip capable of detecting the MS antigen.

The invention discloses a chlamydia trachomatis detection system based on a colloidal gold detection strip, and belongs to the technical field of nucleic acid detection. The chlamydia trachomatis detection system comprises a nucleic acid probe and a colloidal gold detection strip; the nucleic acid probe comprises a probe H1 and a probe H2; the sequence of the probe H1 is as shown in SEQ ID NO. 2; the sequence of the probe H2 is as shown in SEQ ID NO. 3; the colloidal gold detection strip comprises a glass fiber sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a PVC (polyvinyl chloride) bottom plate which are sequentially stacked; and the combination pad is coated with nano microspheres marked by streptavidin. Isothermal non-enzyme amplification of target nucleic acid is achieved through a catalytic hairpin self-assembly (CHA) technology, a colloidal gold detection strip is combined, a developing result is interpreted by naked eyes, detection is achieved, no professional instrument is needed, operation is easy and convenient, detection is rapid, and the result can be interpreted by naked eyes. And the detection system has high sensitivity and high specificity.

This invention belongs to the field of biodetection technology, specifically relating to a monoclonal antibody combination capable of simultaneously recognizing equine and feline serum amyloid A (SAA) and its application. The monoclonal antibody combination comprises 6E10 and 5A6. The CDR sequences of the heavy and light chain variable regions of 6E10 are shown in SEQ ID NO. 1-6, and the CDR sequences of the heavy and light chain variable regions of 5A6 are shown in SEQ ID NO. 7-12. This antibody combination can highly specifically recognize and bind to recombinant equine and feline SAA proteins, achieving multiple uses with a single antibody and reducing raw material development and production costs. The colloidal gold test strip constructed based on this combination exhibits high specificity, high sensitivity, and a wide detection range, with no cross-reactivity. It is easy to operate and requires no complex instruments, providing a stable and reliable biorecognition tool for assessing and monitoring inflammation-related physiological states in animals.

The present invention provides a quality control line coating solution, a quality control line, a test strip, and applications thereof for colloidal gold test strips. The quality control line provided by the present invention uses inexpensive hydrogels to replace commonly used goat anti-mouse, rabbit anti-mouse, and other antibodies in quality control lines. The optimal hydrogel concentration is determined by improving the quality control line of the test strip. The quality control line technology is applicable to the sandwich method and the competitive method in colloidal gold test strip detection, and can effectively intercept colloidal gold and develop color. The general quality control line technology provided by the present invention uses hydrogels for the quality control line of test strip detection, which not only greatly reduces the cost, but also has good universality and can be widely used in colloidal gold test strip detection.

The invention discloses a monoclonal antibody for resisting canine coronavirus N protein, a detection reagent and application of the monoclonal antibody and the detection reagent. The monoclonal antibody for resisting the canine coronavirus N protein comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No. 1; the amino acid sequence of the light chain variable region is as shown in SEQ ID No.2. The monoclonal antibody with high affinity and detection sensitivity to canine coronavirus N protein lays a foundation for research, development and popularization of colloidal gold test strips and fluorescence immunoassay test strips.

The invention belongs to the technical field of virus immunodetection, and relates to a Mab 6B7 antibody, a colloidal gold test strip and application. According to the invention, PDCoV N protein is used as an antigen of a detection line, Mab 6B7 is used as a detection antibody combined with colloidal gold, condition optimization is carried out through different methods, the specificity and sensitivity of the methods are evaluated, and a detection method for detecting the antigen based on an N protein PDCoV colloidal gold competition method is established. The established PDCoV colloidal gold test strip has the advantages of strong specificity, high sensitivity, simplicity and convenience in operation, low production cost and the like, and can be applied to rapid detection of PDCoV in a porcine diarrhea sample clinically.

The application discloses a kind of based on nanobody detection swine epidemic diarrhea virus (PEDV) colloidal gold test strip preparation and application, belong to animal pathogen detection technical field.The application is based on the specific nanobody of 2 strains of anti-PEDV fiber projection glycoprotein screening as basic material, by gene engineering modification to nanobody, they are respectively with the Fc region of pig IgG and ferritin fusion expression, successfully prepared two fusion proteins.Then, with two fusion proteins as matched antibody, wherein the protein labeled with colloidal gold as detection antibody, another protein fused with ferritin is capture antibody, the colloidal gold detection test strip of PEDV is established.The test strip has the advantages of strong specificity, high sensitivity, simple operation, low production cost, etc., and can be applied to the rapid detection of PEDV in clinical pig diarrhea samples.

The present invention discloses a high-precision colloidal gold test strip punching device, specifically relating to the field of punching devices, including a workbench, a support mechanism provided on the top of the workbench, the support mechanism including a driving component and a limit seat, the limit seat being fixedly provided on the workbench, and the driving component being located above the limit seat; a punching mechanism provided on the workbench, the punching mechanism including a movable seat, the movable seat being located below the drive member, the drive member being used to drive the movable seat to move vertically, the movable seat being provided with a punching needle; and a placement slot being provided on the limit seat. The present invention provides a correction mechanism, and according to the position of the test line and the quality control line on the surface of the colloidal gold test strip, the position of the colloidal gold test strip is adjusted by an adapter seat before punching, thereby ensuring that after the colloidal gold test strip is installed in the pregnancy electronic pen, the test line and the quality control line are within the scanning area of the pregnancy electronic pen, thereby ensuring the accuracy of the product test.

The invention discloses an anti-rabies virus G protein monoclonal antibody, a detection reagent and application of the anti-rabies virus G protein monoclonal antibody and the detection reagent. The anti-rabies virus G protein monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No. 1; the amino acid sequence of the light chain variable region is as shown in SEQ ID No.2. The monoclonal antibody provided by the invention has high affinity and detection sensitivity to rabies virus G protein, and lays a foundation for research, development and popularization of colloidal gold test strips and fluorescence immunoassay test strips.

The invention relates to a portable detection device based on constant-temperature PCR reaction. The portable detection device comprises a box body, a constant-temperature heating element, a first stepped pipe barrel, a second stepped pipe barrel, an end cover and a sealing plug, colloidal gold test paper is arranged in the box body, and an observation window is formed in the box body; a liquid outlet is formed in the top of the box body; a tube seat is arranged on the upper surface of the box body; the constant-temperature heating element is arranged on the top surface in the box body; the lower part of the first stepped pipe barrel is connected with the pipe seat, and the bottom surface of the first stepped pipe barrel is provided with a vertically-through reaction bin; the lower portion of the second step pipe barrel is connected with the first step pipe barrel, and a liquid inlet is formed in the bottom face of the second step pipe barrel. The end cover is buckled on the top of the second stepped tube barrel, the end cover is provided with a dropping pipette, and the bottom end of the dropping pipette is located in the liquid inlet; the sealing plug is plugged at a liquid dropping opening of the liquid dropping pipe; the device heats the reaction bin through the constant-temperature heating element, so that the solution and the colloidal gold test paper rapidly react, the detection result is displayed, and the device is small in size, convenient to use and high in detection speed.

The invention belongs to the technical field of biological material structure and performance analysis, and discloses a rapid qualitative and quantitative detection kit for a collagen triple-helix structure and a detection method. The detection kit comprises an enzymolysis system solution A of a non-triple helix structure of specific enzymolysis collagen; an ultrafiltration centrifugal tube; the enzymolysis system liquid B is used for performing non-specific enzymolysis on small-molecule polypeptide; colloidal gold test paper; the colloidal gold test paper is sequentially provided with a sample pad, a combination pad, an NC membrane and a water absorption pad from left to right; the NC membrane is sequentially coated with a T line and a C line from left to right; the marker on the combination pad is a gold-labeled antibody prepared from a hydroxyproline monoclonal antibody and colloidal gold particles through electrostatic coupling; and a coating substance on the T line is a hydroxyproline-carrier protein conjugate. The detection kit provided by the invention realizes a rapid, simple and good-specificity qualitative and quantitative detection method for the collagen triple-helix structure.

This invention discloses a monoclonal antibody against Aeromonas hydrophila and its application. The monoclonal antibody against Aeromonas hydrophila includes monoclonal antibody 275-4 or its antigen-binding portion, and monoclonal antibody 275-5 or its antigen-binding portion. The monoclonal antibody or its antigen-binding portion prepared in this application exhibits high specificity and sensitivity in detecting Aeromonas hydrophila. A colloidal gold test strip prepared using monoclonal antibody 275-4 or its antigen-binding portion and monoclonal antibody 275-5 or its antigen-binding portion can detect Aeromonas hydrophila in biological samples or aquaculture water. This test strip has high sensitivity and specificity, thus exhibiting good detection accuracy. Furthermore, this detection method does not require expensive instruments, is simple and convenient to operate, and can obtain results rapidly in a short time (10-15 minutes), which is beneficial for early disease diagnosis and has good clinical application prospects.