77 results about "Colloidal gold test" patented technology

The invention relates to the technical field of molecular biology, in particular to an avian adenovirus serum type 4 colloidal gold test strip based on a monoclonal antibody and application of the test strip. According to the invention, two monoclonal antibodies (6B3 and 8G11) aiming at FAdV4 penton protein are successfully prepared through a hybridoma technology, and both the monoclonal antibodies show high titer and strong serotype specificity. Specific analysis proves that the two monoclonal antibodies can recognize 12 FAdV4 isolates from different sources, and do not have cross reaction with other FAdV serotypes or common poultry pathogens. According to the invention, through systematic optimization of key parameters, the detection sensitivity of the test strip is 7.81 * 10 < 4 > TCID50 / 0.1 mL, and the detection time is only 15 minutes. Clinical sample verification shows that compared with an ELISA method, the total coincidence rate is 98.6%.

The invention belongs to the technical field of molecular diagnosis, and particularly discloses a high-risk HPV16 rapid detection system based on RPA-CRISPR / Cas12a, which is prepared by the following steps: (1) mixing a nucleic acid lysis solution containing Tris (pH = 8.0), NaHCO3, polyvinylpyrrolidone and Tween-20 with Proteinase K to treat a sample, and performing pyrolysis at room temperature for 5 minutes to obtain a nucleic acid template; (2) an RPA reaction system contains an RNA inhibitor, a reverse transcriptase and an RPA reaction essential enzyme, and after the template and the starting agent in the step (1) are added, RPA amplification is carried out at 37 DEG C; (3) dropping the reaction liquid on a colloidal gold test strip subjected to position exchange and transformation of a sample pad and a water absorption pad, and carrying out result interpretation; and (4) adding the RPA product into a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) reaction system containing the LbCas12a protein, the specific gRNA and the probe. According to the scheme, a rapid, high-sensitivity and cross-reaction-free detection system of the HPV16 is realized by optimizing a nucleic acid lysis solution formula, designing a specific primer and gRNA and modifying a test strip for detection.

The invention discloses a T-2 toxin monoclonal antibody and application thereof, and belongs to the technical field of biology. The monoclonal antibody contains a heavy chain variable region and a light chain variable region, the heavy chain variable region and the light chain variable region are both composed of complementary determining regions and frame regions, and the complementary determining regions are both composed of CDR1, CDR2 and CDR3. The monoclonal antibody can be used for preparing a T-2 toxin detection product. The T-2 toxin colloidal gold test strip provided by the invention has the characteristics of high sensitivity, good specificity and strong stability.

The invention provides a colloidal gold detection method and system based on computer vision, and relates to the technical field of colloidal gold detection.The colloidal gold detection method comprises the steps that a colloidal gold test paper strip image of a to-be-detected sample is collected in real time, the colloidal gold test paper strip image is preprocessed, and a target colloidal gold test paper strip image is obtained; positioning a detection line area and a quality control line area from the target colloidal gold test paper strip image, respectively extracting image data in the detection line area and the quality control line area, constructing a multi-dimensional feature data set, and inputting the multi-dimensional feature data set into a preset colloidal gold concentration detection model to obtain concentration data of the to-be-detected sample, the problem that the accuracy and reliability of existing colloidal gold detection cannot meet the actual application requirements of high-precision quantitative detection is solved, and the effect of improving the accuracy of colloidal gold detection results is achieved.

The invention discloses a deep learning-based colloidal gold test paper result analysis system for detecting renal injury molecular 1. The system comprises an image acquisition module, a region processing module, an effectiveness judgment module, a concentration analysis module and a result generation module, the image acquisition module is used for acquiring a visible light image of the kim-1 colloidal gold test paper, wherein the visible light image at least comprises a detection line area and a quality control line area, and carrying out gray standardization processing, contrast enhancement processing, edge sharpening processing and target area positioning processing on the visible light image; the region processing module is used for performing positioning and boundary segmentation to obtain a mask image which is consistent with the input image in size and is marked with the position of each functional region; the effectiveness judgment module is used for judging whether the kim-1 colloidal gold test paper is effective or not; the concentration analysis module is used for predicting the target substance concentration of the kim-1 colloidal gold test paper; and the result generation module is used for generating an analysis result of the kim-1 colloidal gold test paper and packaging the analysis result into a structured detection report.

The application provides a preparation method of a colloidal gold solution, a preparation method of a colloidal gold labeled protein, a colloidal gold solution and a colloidal gold labeled protein prepared by the method, a kit comprising the colloidal gold solution or the colloidal gold protein, a labeling pad for a colloidal gold test strip, a colloidal gold test strip, and use of the colloidal gold solution, the colloidal gold labeled protein, the labeling pad or the colloidal gold test strip in preparing a kit for detecting a disease marker in a biological sample. For example, the kit of the application can be used for detecting C-reactive protein by a colloidal gold method.

The present disclosure discloses a universal aptamer-based colloidal gold lateral flow test strip for detecting small-molecule substances and belongs to the fields of analytical chemistry, medicine, environment, food safety detection, nano-biosensing and the like. An AuNPs@poly-DNA probe is used for rapidly and sensitively capturing an aptamer, streptavidin sprayed in a test zone and streptavidin-biotin-DNAc sprayed in a control zone do not need to be changed, and another substance can be detected only by changing a nucleic acid chain part of the AuNPs@poly-DNA probe. A universal colloidal gold lateral flow test strip, which is rapid, sensitive and low in cost, has been developed. The test strip method for detecting small-molecule substances is simple, convenient and rapid, and can be used for detection at any time. Only a test solution is needed to be added into a sample port and the test strip is completely developed after 5 min, thus an experiment result can be observed and the detection efficiency can be greatly improved. Qualitative analysis can be conducted by naked eyes, and quantitative analysis can be conducted by a colloidal gold test strip quantitative analyzer.

The invention discloses a feline leukemia virus p27 protein monoclonal antibody, a hybridoma cell strain and application thereof, and relates to the technical field of biology, the amino acid sequence of a heavy chain variable region of the monoclonal antibody is as shown in SEQ ID NO.1 in a sequence table, and the amino acid sequence of a light chain variable region of the monoclonal antibody is as shown in SEQ ID NO.2 in the sequence table. According to the invention, a cell fusion technology is utilized to establish a hybridoma cell strain p27-3A5 capable of secreting a p27 protein monoclonal antibody, and a specific monoclonal antibody 3A5 is obtained. And the minimum linear epitope identified by the antibody is identified, so that the establishment of subsequent preparation of a diagnostic kit such as a colloidal gold test strip is facilitated.

The application discloses a colloidal gold test strip for distinguishing between wild toxin infection and vaccine immunization of porcine pleuropneumonia actinobacillus and application. The test strip of the application takes ApxIVA truncated protein with an amino acid sequence shown as SEQ ID NO. 3 as an antigen, the truncated protein can be soluble expressed in E. coli, and the structure and function of the truncated protein are close to those of natural apxIVA. The test strip of the application comprises a sample pad, a colloidal gold pad, an NC membrane, a water absorption filter paper and a bottom plate, the colloidal gold pad is coated with gold-labeled apxIVA truncated protein and gold-labeled chicken IgY antibody, and the C line and the T line of the NC membrane are fixed with goat anti-chicken IgY antibody and apxIVA truncated protein. The test strip only needs 25 muL of pig serum to make detection within 5-10 min, and can distinguish between APP wild strain infection and gene deletion vaccine APP-HB-04M immunized serum samples.

The present application relates to the technical field of biological medicine detection, and discloses a colloidal gold test strip for detecting anti-PLA2R antibody, a preparation method, a kit and application thereof.Capture antigens and detection antigens of the colloidal gold test strip are both PLA2R proteins, the nucleotide sequence of the PLA2R protein is shown as SEQ ID No.1, and the amino acid sequence of the PLA2R protein is shown as SEQ ID No.2.The colloidal gold test strip for detecting anti-PLA2R antibody and the kit have the characteristics of simplicity, good stability, high sensitivity, good specificity, simple operation, suitability for on-site rapid detection, rapid result output and directness, and can be applied to family and bedside detection without special laboratory and experimental personnel.

The invention relates to the technical field of veterinary biology, in particular to a monoclonal cell strain related to mycoplasma synoviae, a test strip and a preparation method and application of the monoclonal cell strain and the test strip. A heavy chain VH nucleotide sequence of a chicken mycoplasma synoviae antibody secreted by the monoclonal cell strain MS1C5-F8 is SEQ ID NO.1, an amino acid sequence of the chicken mycoplasma synoviae antibody is SEQ ID NO.3, a light chain VL nucleotide sequence of the chicken mycoplasma synoviae antibody is SEQ ID NO.2, and an amino acid sequence of the chicken mycoplasma synoviae antibody is SEQ ID NO.4. The chicken mycoplasma synoviae antibody not only can be subjected to specific reaction with different MS clinical isolate antigens, but also has no cross reaction with other common avian bacterial pathogens. The monoclonal antibody cell strain is used for immunizing a mouse to prepare ascites and purifying a corresponding antibody, and the purified monoclonal antibody is used for successfully preparing a colloidal gold test strip capable of detecting the MS antigen.

This invention belongs to the field of biodetection technology, specifically relating to a monoclonal antibody combination capable of simultaneously recognizing equine and feline serum amyloid A (SAA) and its application. The monoclonal antibody combination comprises 6E10 and 5A6. The CDR sequences of the heavy and light chain variable regions of 6E10 are shown in SEQ ID NO. 1-6, and the CDR sequences of the heavy and light chain variable regions of 5A6 are shown in SEQ ID NO. 7-12. This antibody combination can highly specifically recognize and bind to recombinant equine and feline SAA proteins, achieving multiple uses with a single antibody and reducing raw material development and production costs. The colloidal gold test strip constructed based on this combination exhibits high specificity, high sensitivity, and a wide detection range, with no cross-reactivity. It is easy to operate and requires no complex instruments, providing a stable and reliable biorecognition tool for assessing and monitoring inflammation-related physiological states in animals.

The invention discloses a monoclonal antibody for resisting canine coronavirus N protein, a detection reagent and application of the monoclonal antibody and the detection reagent. The monoclonal antibody for resisting the canine coronavirus N protein comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No. 1; the amino acid sequence of the light chain variable region is as shown in SEQ ID No.2. The monoclonal antibody with high affinity and detection sensitivity to canine coronavirus N protein lays a foundation for research, development and popularization of colloidal gold test strips and fluorescence immunoassay test strips.

The invention belongs to the technical field of virus immunodetection, and relates to a Mab 6B7 antibody, a colloidal gold test strip and application. According to the invention, PDCoV N protein is used as an antigen of a detection line, Mab 6B7 is used as a detection antibody combined with colloidal gold, condition optimization is carried out through different methods, the specificity and sensitivity of the methods are evaluated, and a detection method for detecting the antigen based on an N protein PDCoV colloidal gold competition method is established. The established PDCoV colloidal gold test strip has the advantages of strong specificity, high sensitivity, simplicity and convenience in operation, low production cost and the like, and can be applied to rapid detection of PDCoV in a porcine diarrhea sample clinically.

The invention discloses an anti-rabies virus G protein monoclonal antibody, a detection reagent and application of the anti-rabies virus G protein monoclonal antibody and the detection reagent. The anti-rabies virus G protein monoclonal antibody comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No. 1; the amino acid sequence of the light chain variable region is as shown in SEQ ID No.2. The monoclonal antibody provided by the invention has high affinity and detection sensitivity to rabies virus G protein, and lays a foundation for research, development and popularization of colloidal gold test strips and fluorescence immunoassay test strips.

The invention relates to a portable detection device based on constant-temperature PCR reaction. The portable detection device comprises a box body, a constant-temperature heating element, a first stepped pipe barrel, a second stepped pipe barrel, an end cover and a sealing plug, colloidal gold test paper is arranged in the box body, and an observation window is formed in the box body; a liquid outlet is formed in the top of the box body; a tube seat is arranged on the upper surface of the box body; the constant-temperature heating element is arranged on the top surface in the box body; the lower part of the first stepped pipe barrel is connected with the pipe seat, and the bottom surface of the first stepped pipe barrel is provided with a vertically-through reaction bin; the lower portion of the second step pipe barrel is connected with the first step pipe barrel, and a liquid inlet is formed in the bottom face of the second step pipe barrel. The end cover is buckled on the top of the second stepped tube barrel, the end cover is provided with a dropping pipette, and the bottom end of the dropping pipette is located in the liquid inlet; the sealing plug is plugged at a liquid dropping opening of the liquid dropping pipe; the device heats the reaction bin through the constant-temperature heating element, so that the solution and the colloidal gold test paper rapidly react, the detection result is displayed, and the device is small in size, convenient to use and high in detection speed.

The invention belongs to the technical field of biological material structure and performance analysis, and discloses a rapid qualitative and quantitative detection kit for a collagen triple-helix structure and a detection method. The detection kit comprises an enzymolysis system solution A of a non-triple helix structure of specific enzymolysis collagen; an ultrafiltration centrifugal tube; the enzymolysis system liquid B is used for performing non-specific enzymolysis on small-molecule polypeptide; colloidal gold test paper; the colloidal gold test paper is sequentially provided with a sample pad, a combination pad, an NC membrane and a water absorption pad from left to right; the NC membrane is sequentially coated with a T line and a C line from left to right; the marker on the combination pad is a gold-labeled antibody prepared from a hydroxyproline monoclonal antibody and colloidal gold particles through electrostatic coupling; and a coating substance on the T line is a hydroxyproline-carrier protein conjugate. The detection kit provided by the invention realizes a rapid, simple and good-specificity qualitative and quantitative detection method for the collagen triple-helix structure.

This invention discloses a monoclonal antibody against Aeromonas hydrophila and its application. The monoclonal antibody against Aeromonas hydrophila includes monoclonal antibody 275-4 or its antigen-binding portion, and monoclonal antibody 275-5 or its antigen-binding portion. The monoclonal antibody or its antigen-binding portion prepared in this application exhibits high specificity and sensitivity in detecting Aeromonas hydrophila. A colloidal gold test strip prepared using monoclonal antibody 275-4 or its antigen-binding portion and monoclonal antibody 275-5 or its antigen-binding portion can detect Aeromonas hydrophila in biological samples or aquaculture water. This test strip has high sensitivity and specificity, thus exhibiting good detection accuracy. Furthermore, this detection method does not require expensive instruments, is simple and convenient to operate, and can obtain results rapidly in a short time (10-15 minutes), which is beneficial for early disease diagnosis and has good clinical application prospects.

The invention is applicable to the technical field of animal virus detection, and provides a PVC2 and PRRSV (porcine reproductive and respiratory syndrome virus) double colloidal gold test strip and application thereof. Ferritin nanocage fusion protein of PCV2 and PRRSV is taken as a capture antibody; a gold-labeled PCV2 monoclonal antibody and a gold-labeled PRRSV monoclonal antibody are used as detection antibodies, and the gold-labeled PCV2 monoclonal antibody and the gold-labeled PRRSV monoclonal antibody are obtained by labeling a specific monoclonal antibody composition by using a gold labeling technology and treating the specific monoclonal antibody composition. The prepared double colloidal gold test strip is high in specificity, low in production cost, easy to operate and visual in result, PCV2 and PRRSV infection can be rapidly identified and diagnosed on site at the same time, and the double colloidal gold test strip has wide market application prospects.

The utility model discloses a colloidal gold test paper card for rapidly determining rice yeast acid in whole blood. The colloidal gold test paper card comprises a bottom plate as well as a glass cellulose membrane, a filtration binding area, a detection area and a water absorption pad which are sequentially arranged on the bottom plate from left to right, the filtration binding area adopts an antigen-antibody binding technology, red blood cells in whole blood can be retained on a filter membrane of a sample pad without moving, other substances in the whole blood can smoothly pass through, and the detection effect is good. According to the present invention, the kit can be combined with the rice yeast acid so as to eliminate the background interference, can be combined with the rice yeast acid, and can be detected through the detection region, such that the rice yeast acid in the whole blood can be accurately detected, the accuracy and the specificity of the rice yeast acid determination in the whole blood can be effectively improved, the application range and the efficiency of the trace whole blood sample in the detection can be expanded, and the redundant pretreatment operation is not required; the method is simple in operation and rapid in detection, and can obtain a result within 15 minutes only by taking 10 microliters of trace whole blood, and the lowest detection limit of 50 micrograms / L is reached.

The invention relates to an antibody specifically binding to a novel coronavirus N protein or an antigen binding fragment thereof and application thereof. The antibody or the antigen binding fragment thereof has improved detection sensitivity and specificity, and the related colloidal gold test strip is convenient for rapid detection and operation, low in detection cost and wide in applicability.

Provided in the present invention is a food safety test apparatus, comprising a multi-station turntable mechanism, test devices and an extraction device. The multi-station turntable mechanism comprises a turntable I and a first driving mechanism, the turntable I being provided with a first holding portion. The test devices include a first test device and a second test device, wherein the first test device is provided with a placement slot for accommodating a multi‑panel colloidal gold test cassette and a first image acquisition unit, and the second test device is provided with a second holding portion for accommodating a colloidal gold test cassette and a second image acquisition unit. The extraction device comprises a mounting plate I, a liquid suction and discharge device and a second driving mechanism, wherein the liquid suction and discharge device comprises a needle tube and a liquid pump I, and the liquid pump I sucks in and discharges liquid via the needle tube. The second driving mechanism is configured to drive the mounting plate I to move so as to drive the needle tube to transfer among the first holding portion, the placement slot and the second holding portion. The present invention has the advantages of being capable of extracting samples to be tested, supporting the simultaneous testing of a plurality of samples, providing test results with high accuracy, and integrating a plurality of test methods.

The utility model relates to the technical field of colloidal gold detection, in particular to a colloidal gold test strip detector which comprises a shell, a control module, a test strip conveying module, a reagent filling module, an infrared laser heating module, an image acquisition module and a thermal imaging module, the test strip conveying module, the reagent filling module, the image acquisition module, the infrared laser heating module, the thermal imaging module and the control module are all mounted in the shell, and the test strip conveying module, the reagent filling module, the infrared laser heating module, the image acquisition module and the thermal imaging module are all connected with the control module; and the shell is also provided with an insertion hole into which the colloidal gold test strip can extend. For colloidal gold test strips capable of absorbing infrared bands and developing at the same time, the colloidal gold test strip detector disclosed by the utility model can be used for developing detection and infrared detection at the same time, so that the detection period of the colloidal gold test strips is shortened, colloidal gold particles can be prevented from being oxidized in the detection process, and the accuracy of a detection result is improved.

The invention provides a method for distinguishing immunized BVDV (bovine viral diarrhea virus) inactivated vaccine from wild virus infection, namely a method for distinguishing inactivated vaccine immunized cattle or wild virus infected cattle, which is established on the basis of screening to obtain BVDV specific antigen fusion protein. The amino acid sequence of the antigen fusion protein is SEQ ID NO: 1. The fusion polypeptide provided by the invention can be used as a specific antigen for colloidal gold immunochromatography detection, and the prepared colloidal gold test strip can effectively distinguish BVDV wild virus infection from inactivated vaccine immunity, and has high specificity and sensitivity. The provided fusion polypeptide is designed based on the minimum dominant epitope, interference of irrelevant protein sequences is eliminated, the detection specificity and accuracy are improved, and the fusion polypeptide is suitable for on-site rapid detection.

The utility model discloses a colloidal gold immunochromatography analyzer with a test paper pop-up structure, which relates to the technical field of colloidal gold immunochromatography analyzers and comprises an analyzer shell and a connecting seat, and the head end of the connecting seat is telescopically mounted in the analyzer shell. A telescopic guide mechanism for limiting the telescopic speed of the connecting seat is mounted in the analyzer shell and mainly comprises a cylinder barrel, an oil tank, a push rod, a piston, a three-way pipe and a throttling bolt; according to the utility model, the telescopic speed of the connecting seat is limited by the telescopic guide mechanism, so that the colloidal gold test card in the connecting seat is effectively prevented from shaking due to rapid movement; in the detection process, a sample is prevented from being interfered by migration on a membrane in the colloidal gold test card, the stability of an antigen-antibody binding process is ensured, and the risk of incomplete reaction or non-specific binding is reduced, so that the accuracy of a detection result is remarkably improved.

This invention provides a method and device for interpreting colloidal gold test strips. The detection method includes: after the test strip collects the sample to be tested, waiting for the test strip to complete the reaction; moving the test strip or the collection device so that the detection area or the sensor collection area moves along a first direction in a preset manner, wherein the collection device collects the reflected light signal of the test strip in a preset manner to obtain signal data covering the control part and the detection part; the collection device extracts the signal data and calculates the abrupt change characteristic value of the signal data to obtain the detection result.

This invention belongs to the field of biodetection technology, specifically relating to a monoclonal antibody combination that specifically recognizes the hemagglutinin protein of the H6 subtype of avian influenza virus and its applications. The antibody combination consists of monoclonal antibodies 2D12 and 4G3, targeting different epitopes of the H6 hemagglutinin protein, and possesses clearly defined heavy and light chain variable region complementarity-determining region sequences, as shown in SEQ ID NO. 1-12, respectively. The antibody combination exhibits high specificity and affinity, effectively avoiding cross-reactivity with other influenza subtypes such as H1, H5, H7, and H9, as well as common avian pathogens. The colloidal gold test strip constructed based on this antibody combination is highly sensitive and easy to operate, suitable for in vitro detection and epidemiological screening of the H6 subtype of avian influenza virus. It solves the false positive problem caused by cross-reactivity in existing technologies, providing a reliable technical means for the monitoring and quarantine of the H6 subtype of virus.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody for resisting avian influenza virus H7 subtype hemagglutinin protein and application. The amino acid sequence of a heavy chain variable region CDR of the monoclonal antibody 1F4 is shown as SEQ ID NO.1-3, the amino acid sequence of a light chain variable region CDR of the monoclonal antibody 1F4 is shown as SEQ ID NO.4-6, and the heavy chain variable region, the light chain variable region and coding nucleotide sequences of the monoclonal antibody 1F4 are clear. The monoclonal antibody has high specificity and good affinity, can effectively distinguish the H7 subtype from other influenza virus subtypes, and has no cross reaction. The invention also provides application of the colloidal gold test strip in preparation of a recognition tool, the constructed colloidal gold test strip takes 1F4 as a capturing and labeling antibody, is simple and convenient to operate, visual in result and suitable for on-site rapid screening and large-scale monitoring, and provides technical support for prevention and control of H7 subtype avian influenza.

The present application relates to the technical field of in vitro detection, and more particularly to a colloidal gold test strip for detecting pig whole blood, wherein the sample pad of the colloidal gold test strip is coated with a complex antibody of anti-pig red blood cell antibody and anti-pig hemoglobin antibody, which can directly bind to red blood cells and hemoglobin in pig whole blood, eliminating the interference of hemolysis in whole blood detection on the result. The colloidal gold test strip has the advantages of simple operation, good serum separation effect, small sample dosage, fast detection speed and the like, and has a wide application prospect in the field detection of pig epidemic.

The application discloses a pair of single-domain heavy chain antibody-based immunochromatographic test strip and a method for detecting a novel coronavirus receptor binding domain protein, and belongs to the field of immunochromatographic detection.The immunofluorescence test strip provided by the application comprises a PVC bottom plate, a sample pad, a binding pad, an NC membrane and a water absorption pad, donor microspheres-single chain heavy domain antibody W is spotted on the binding pad, and receptor microspheres-single domain heavy chain antibody Q and anti-His monoclonal antibody are respectively line-marked on a detection line and a quality control line; RBD protein is prepared from mammalian cells, and single-domain heavy chain antibody is prepared by prokaryotic expression; the antibodies are respectively coupled to the surfaces of the donor microspheres and the receptor microspheres, 0.01-1000 ng / mL novel coronavirus RBD protein is loaded to prepare the test strip, and the sensitivity of the test strip is evaluated.The immunofluorescence test strip provided by the application can rapidly and sensitively detect the novel coronavirus receptor binding domain protein, and compared with a commercially available colloidal gold test strip for detecting a novel coronavirus antigen, has higher sensitivity, better detection accuracy and stability and lower detection cost.