190 results about "Colloidal gold test" patented technology

The invention discloses a monoclonal antibody for identifying canine distemper virus H protein, a detection test strip and application. The monoclonal antibody comprises a monoclonal antibody 5-8H or a monoclonal antibody 9-7B and has high specificity and sensitivity, the two antibodies can recognize different epitopes, and a detection test strip can be prepared through a double-antibody sandwich method. Tests prove that the monoclonal antibody provided by the invention has no obvious cross reaction on canine parvovirus, canine adenovirus, canine coronavirus and canine parainfluenza virus in preparation of a colloidal gold test strip for detecting the canine distemper virus, and has good specificity and higher sensitivity.

The invention discloses an intelligent colloidal gold test strip result identification and analysis method based on machine learning, particularly relates to the technical field of image detection, and is used for solving the problem of lack of analysis on a time dynamic rule of a developing process in the prior art. The method comprises the following steps: acquiring color development images of a test strip at different time points, extracting local features and global features of the color development images, and forming color development change feature vectors based on adaptive weighted fusion of feature weights; further constructing a time dynamic characteristic model of the color development reaction based on the color development change characteristic vector, and analyzing a time dynamic rule of the color development process; the method comprises the following steps: judging the stability of the morphological change of a color development region through morphological filtering and an active contour model, and calculating and evaluating the dynamic trend of a color development signal in combination with Shannon entropy; the time dynamic characteristic model is used for predicting the developing result and outputting the detection result of the test strip, so that the detection accuracy of the test strip is improved.

The invention provides colloidal gold test paper, a detection method and device thereof and a computer readable storage medium, and relates to the field of rapid test paper detection.The colloidal gold test paper is printed with a first positioning block and a second positioning block on the two sides of a color developing area, and the method comprises the steps that a target image containing the complete colloidal gold test paper to be detected is obtained; performing positioning block positioning on the target image to obtain position information of the two positioning blocks; acquiring a color development area image based on the position information, further obtaining a detection line image and a quality control line image, performing image preprocessing, and then extracting feature vectors of a detection line and a quality control line; and finally, the cloud server obtains a detection result by using the classification prediction model and returns the detection result. According to the method, two positioning blocks are printed on the test paper, so that a user can automatically identify the accurate position of the colloidal gold test paper and obtain a real-time detection result by using a mobile terminal on the premise of almost not increasing the production cost.

The method comprises the following steps: collecting a group of raw milk submission test information table images, and intercepting five types of bigeminy colloidal gold test paper on each submission test information table as a to-be-detected data set; replacing a C2f module of the YOLOv8 backbone network with an irregular feature extraction module, adding a position information attention module at the tail of the backbone network, and constructing to obtain a test paper color development recognition model; training the test paper color development recognition model based on an improved WIoU loss function and a self-defined sample set containing different color development conditions; inputting the to-be-detected data set into a trained test paper color development recognition model for recognition to obtain a plurality of prediction frames; and determining a regional color development recognition result by comparing the sizes of the projections accumulated by the pixel values on the same vertical coordinates of the plurality of prediction frames. According to the invention, a target detection technology is applied to an immunochromatography test paper color development recognition task, and a new thought and a solution are provided for color development recognition of batch immunochromatography test paper.

The invention relates to the technical field of molecular biology, in particular to an avian adenovirus serum type 4 colloidal gold test strip based on a monoclonal antibody and application of the test strip. According to the invention, two monoclonal antibodies (6B3 and 8G11) aiming at FAdV4 penton protein are successfully prepared through a hybridoma technology, and both the monoclonal antibodies show high titer and strong serotype specificity. Specific analysis proves that the two monoclonal antibodies can recognize 12 FAdV4 isolates from different sources, and do not have cross reaction with other FAdV serotypes or common poultry pathogens. According to the invention, through systematic optimization of key parameters, the detection sensitivity of the test strip is 7.81 * 10 < 4 > TCID50 / 0.1 mL, and the detection time is only 15 minutes. Clinical sample verification shows that compared with an ELISA method, the total coincidence rate is 98.6%.

The invention relates to the technical field of pesticide detection, and discloses a multi-component colloidal gold test strip for rapid detection of organophosphorus as well as a preparation method and application thereof, the preparation method comprises the following steps: preparing a specific antibody of the organophosphorus, and preparing a colloidal gold solution at the same time; mixing and incubating the specific antibody and a colloidal gold solution to obtain an antibody-labeled colloidal gold solution; pre-treating a detection pad, and immersing the detection pad in the antibody-labeled colloidal gold solution to obtain an impregnated pad; and sequentially assembling the dried dipping pad, a water absorption pad, a sample pad and a combination pad on a bottom plate to form a complete test strip. By improving the preparation process of the antibody and the assembly technology of the test strip, the detection sensitivity and specificity are remarkably improved. The test strip is simple and convenient in preparation process, high in cost benefit and convenient for large-scale production. In addition, the test strip is convenient to use and does not need to depend on professional equipment or technicians, and the detection result is visual and convenient to read.

The invention relates to a hybridoma cell strain capable of secreting a napropamide-resistant monoclonal antibody and application of the hybridoma cell strain, and belongs to the field of food safety immunodetection. The hybridoma cell strain Tau4 secreting the napropamide cloned antibody is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is October 31, 2024, and the preservation number is CGMCC No.46242. The hybridoma cell strain Tau4 secreting the napropamide cloned antibody has the advantages that the hybridoma cell strain Tau4 secreting the napropamide The napropamide-resistant monoclonal antibody secreted by the cell strain is used for analyzing and detecting napropamide residues in food safety detection. The napropamide-resistant monoclonal antibody cell strain obtained by the invention can be used for immunoassay detection, and has better detection sensitivity and specificity (IC50 value is 1.13 ng / mL) on napropamide. The result of the invention can be used for preparing an immunodetection kit and a colloidal gold test strip of napropamide, and provides a powerful detection method and means for detecting the residual quantity of napropamide in foods such as fruits, vegetables and the like.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for detecting candida albicans enolase protein and application. And the monoclonal antibody combination comprises monoclonal antibodies 5G7 and 5B1. The amino acid sequences of CDR regions of the monoclonal antibody 5G7 and the monoclonal antibody 5B1 are as shown in SEQ ID NO.1-12. The antibody combination has high specificity and binding activity, and can be used for efficient detection of candida albicans enolase. The colloidal gold test strip constructed on the basis of the combination has good sensitivity and specificity, the lowest detection limit can reach 500pg / ml, and the colloidal gold test strip does not have cross reaction with other common fungi. The invention provides a reliable tool for early diagnosis and rapid detection of invasive candida infection, and has a good clinical application prospect.

The invention discloses a monoclonal antibody and application thereof in quantitative detection of porcine epidemic diarrhea virus, and belongs to the technical field of biological detection. The preparation method comprises the following steps: firstly, obtaining two monoclonal antibodies GC44F3-1 and GC32C11-5 which specifically react with S protein of the porcine epidemic diarrhea virus; secondly, establishing a double-antibody sandwich ELISA (Enzyme-Linked Immunosorbent Assay) method by taking the two monoclonal antibodies as a coating antibody and a detection antibody respectively, and applying the double-antibody sandwich ELISA method to PEDV (Porcine Epidemic Diarrhea Virus) vaccine S protein quantification; the method can be used for detecting semi-finished products and finished products, can be used for process establishment, optimization, vaccine quality evaluation and stability monitoring of porcine epidemic diarrhea virus vaccine antigens, has the advantages of high sensitivity, high accuracy, high repeatability and high flux, is high in detection flux and high in speed, solves the problem that TCID50 cannot meet vaccine quality evaluation at present, and has a good application prospect. The defect that the existing S protein ELISA method can only be used for qualitative judgment is overcome. The monoclonal antibody can also be used for blocking ELISA, capturing ELISA, indirect ELISA, competitive ELISA and a reagent for detecting the porcine epidemic diarrhea virus through a colloidal gold test strip.

The invention belongs to the technical field of molecular diagnosis, and particularly discloses a high-risk HPV16 rapid detection system based on RPA-CRISPR / Cas12a, which is prepared by the following steps: (1) mixing a nucleic acid lysis solution containing Tris (pH = 8.0), NaHCO3, polyvinylpyrrolidone and Tween-20 with Proteinase K to treat a sample, and performing pyrolysis at room temperature for 5 minutes to obtain a nucleic acid template; (2) an RPA reaction system contains an RNA inhibitor, a reverse transcriptase and an RPA reaction essential enzyme, and after the template and the starting agent in the step (1) are added, RPA amplification is carried out at 37 DEG C; (3) dropping the reaction liquid on a colloidal gold test strip subjected to position exchange and transformation of a sample pad and a water absorption pad, and carrying out result interpretation; and (4) adding the RPA product into a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) reaction system containing the LbCas12a protein, the specific gRNA and the probe. According to the scheme, a rapid, high-sensitivity and cross-reaction-free detection system of the HPV16 is realized by optimizing a nucleic acid lysis solution formula, designing a specific primer and gRNA and modifying a test strip for detection.

The invention discloses a colloidal gold test strip for distinguishing porcine actinobacillus pleuropneumoniae wild virus infection and vaccine immunity and application. According to the test strip, ApxIVA truncated protein with the amino acid sequence shown as SEQ ID NO.3 serves as an antigen, the truncated protein can be subjected to soluble expression in escherichia coli, and the structure and the function of the truncated protein are close to those of natural apxIVA. The test strip comprises a sample pad, a colloidal gold pad, an NC membrane, water absorption filter paper and a bottom plate, the colloidal gold pad is coated with gold-labeled apxIVA truncated protein and a gold-labeled chicken IgY antibody, and a goat anti-chicken IgY antibody and apxIVA truncated protein are fixed on a C line and a T line of the NC membrane. The method can be used for distinguishing APP wild strain infection and gene deletion seedling APP-HB-04M immune serum samples.

The invention provides a primer group and a kit for detecting bovine mycobacterium paratuberculosis based on a recombinase isothermal amplification technology and application, and relates to the technical field of biological detection.The primer group comprises a forward primer MAP-F3, a reverse primer MAP-R3 and a specific fluorescent probe MAP-P3 and has good specificity; the sensitivity reaches 10fg / mu L, which is more sensitive than that of a colloidal gold test strip, the dependence on equipment is broken through, and the detection result is more accurate. The technical problem that in the prior art, it is difficult to distinguish bovine mycobacterium paratuberculosis from other pathogens through a detection method, or the detection process is tedious is solved. According to the prepared reagent or kit, the liquid preparation process is simple, the whole amplification process can be completed only in 30 min, the amplification yield can reach 109-1010 copies, the method is rapid and efficient, the amplification result can be directly judged according to fluorescence data, other auxiliary means are not needed, and the method is suitable for field detection.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody pair for detecting cat immunodeficiency virus p24 protein and application of the monoclonal antibody pair. The monoclonal antibody pair is a monoclonal antibody 1D8 and a monoclonal antibody 1H10 and can specifically recognize cat immunodeficiency virus p24 protein, and sequences of complementary determining regions of variable regions of a heavy chain and a light chain of the 1D8 and the 1H10 are clear and are respectively shown as SEQ ID NO.1-12. The antibody pair can effectively avoid cross reaction, and the accuracy and sensitivity of detection are remarkably improved; the colloidal gold test strip constructed on the basis of the antibody pair is simple, convenient and rapid to operate, is suitable for early antigen detection of FIV infection, solves the problem that natural infection and vaccine immunity cannot be distinguished in existing antibody detection, and provides a reliable technical means for FIV field screening and disease prevention and control.

The invention discloses a T-2 toxin monoclonal antibody and application thereof, and belongs to the technical field of biology. The monoclonal antibody contains a heavy chain variable region and a light chain variable region, the heavy chain variable region and the light chain variable region are both composed of complementary determining regions and frame regions, and the complementary determining regions are both composed of CDR1, CDR2 and CDR3. The monoclonal antibody can be used for preparing a T-2 toxin detection product. The T-2 toxin colloidal gold test strip provided by the invention has the characteristics of high sensitivity, good specificity and strong stability.

The invention discloses a kit, application and method for rapidly detecting mycoplasma synoviae based on an ERA-CRISPR / Cas12a one-step method, according to the visual detection kit, on the basis of a new nucleic acid detection target scpA of the mycoplasma synoviae, a sample to be detected is split by a splitting buffer to release genome DNA, then ERA-CRISPR / Cas12a one-pot reaction is carried out in a constant-temperature water bath kettle or a metal bath, and the sample to be detected is subjected to one-pot reaction, so that the ERA-CRISPR / Cas12a one-pot reaction is carried out to rapidly detect the mycoplasma synoviae through the ERA-CRISPR / Cas12a one-pot reaction. Under the mediation of a crRNA guide sequence, after Lb Cas12a enzyme is combined with crRNA and target DNA to form a ternary complex, the trans-shear activity of cas12a is activated, an ssDNA fluorescent probe in a reaction system can be arbitrarily cut to release a fluorescent group on the probe, and an ultraviolet lamp is used for detecting a fluorescent signal or a colloidal gold test strip is used for detecting whether FAM and Biotin groups are cut off for result judgment. According to the ERA-CRISPR / cas12a one-pot method technology for rapidly detecting the mycoplasma synoviae, the DNA target of the mycoplasma synoviae is recognized and cut through ERA amplification and CRISPR / cas12a specificity, the specificity is high, the detection sensitivity is as low as 3.81 pg / mu L, and the ERA-CRISPR / cas12a one-pot method technology can be used for rapidly detecting the mycoplasma synoviae in clinical samples on site.

The invention discloses a reagent for nucleic acid extraction and amplification of porcine epidemic diarrhea virus and application, a nucleic acid rapid extraction technology part is a nucleic acid rapid extraction reagent, and the reagent mainly comprises tris (hydroxymethyl) aminomethane (Tris), TritonX-100 (TritonX-100) and chelating resin 100 (Chelex100). The PEDV on-site rapid detection technology comprises an RT-RAA reaction system and a colloidal gold test strip. The RT-RAA reaction system is composed of an RT-RAA nucleic acid amplification reagent, a pair of primers and a probe, and the primers and the probe are used for identifying an N gene conserved region of PEDV. The kit can complete virus nucleic acid extraction and nucleic acid in-vitro amplification and detection within 30 min, the specificity is good, and the detection sensitivity is 101 copies / microliter. The kit disclosed by the invention has the characteristics of simplicity in operation, rapidness and sensitivity, and is suitable for on-site extraction of a large batch of samples and on-site rapid diagnosis of PEDV (Porcine Epidemic Diarrhea Virus).

The invention provides a colloidal gold detection method and system based on computer vision, and relates to the technical field of colloidal gold detection.The colloidal gold detection method comprises the steps that a colloidal gold test paper strip image of a to-be-detected sample is collected in real time, the colloidal gold test paper strip image is preprocessed, and a target colloidal gold test paper strip image is obtained; positioning a detection line area and a quality control line area from the target colloidal gold test paper strip image, respectively extracting image data in the detection line area and the quality control line area, constructing a multi-dimensional feature data set, and inputting the multi-dimensional feature data set into a preset colloidal gold concentration detection model to obtain concentration data of the to-be-detected sample, the problem that the accuracy and reliability of existing colloidal gold detection cannot meet the actual application requirements of high-precision quantitative detection is solved, and the effect of improving the accuracy of colloidal gold detection results is achieved.

The invention discloses an AIE fluorescence immunochromatography test strip for detecting a toxoplasma gondii antibody as well as a preparation method and application of the AIE fluorescence immunochromatography test strip, and belongs to the technical field of production of veterinary biological diagnosis products. The test strip provided by the invention comprises a bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad, the combination pad, the nitrocellulose membrane and the water absorption pad are bonded on the bottom plate in an end-to-end connection manner; the combination pad is coated with AIE microsphere labeled protein; the nitrocellulose membrane contains a detection line T and a quality control line C; the test strip prepared by adopting a double-antigen sandwich antibody detection method can be used for detecting serum antibodies in animal bodies such as pigs, cats and the like. Compared with a colloidal gold test strip on the market, the colloidal gold test strip has the advantages that the sensitivity is improved by 32 times, the batch-to-batch difference is less than 5%, the test performed by adopting 40 parts of pig serum and 30 parts of cat serum shows that the specificity, the stability and the accuracy are excellent, the operation is simple, the cost is low, and an accurate result can be obtained only in 10 minutes; and a rapid and effective detection method is provided for epidemiological investigation and disease diagnosis.

The invention relates to a colloidal gold test strip for detecting a sheep pox virus antibody as well as a preparation method and application of the colloidal gold test strip. According to the invention, firstly, a recombinant 122 protein is prepared, and the recombinant 122 protein has remarkable antigenicity and immunogenicity and can be used as a good target for diagnosing the sheep pox virus; secondly, the colloidal gold test strip for detecting the sheep pox virus antibody is prepared, a sample pad, a gold-labeled pad, a nitrocellulose membrane and a water absorption pad are sequentially arranged on a PVC base plate of the test strip, and the gold-labeled pad is coated with gold-labeled recombinant 122 protein; the test strip can simultaneously detect antibodies of the sheep pox virus, the bovine nodular skin disease virus and the goat pox virus, the sensitivity can reach 1: 128, 1: 128 and 1: 64 respectively, the test strip has no cross reaction with bovine viral diarrhea virus, foot-and-mouth disease O-type virus, foot-and-mouth disease A-type virus, orf virus, brucellosis and colibacillosis, the specificity is good, and the diagnosis coincidence rate reaches 93%.

The invention discloses a porcine epidemic diarrhea virus S protein monoclonal antibody and application thereof. Specifically, two monoclonal antibodies GC42G2-1 and GC33D8-1 which have broad-spectrum binding characteristics with the porcine epidemic diarrhea virus S protein are obtained firstly, the GC42G2-1 antibody has good universality and can have strong affinity with different strains, and the GC33D8-1 antibody can be used for binding the porcine epidemic diarrhea virus S protein in a broad-spectrum manner. The GC42G2-1 can be independently used, and also can be matched with a polyclonal antibody or GC33D8-1 to be used for sandwich ELISA (enzyme-linked immunosorbent assay) so as to detect and quantify PEDV (porcine epidemic diarrhea virus). Secondly, the two monoclonal antibodies are respectively used as a coating antibody and a detection antibody, a double-antibody sandwich ELISA method is established, and the double-antibody sandwich ELISA method is applied to PEDV vaccine S protein quantification, has better parallelism compared with other antibodies, and can be widely applied to detection of different strains. The method disclosed by the invention has the advantages of high sensitivity, high accuracy, high repeatability and high flux, and can be used for the development of blocking ELISA (Enzyme-Linked Immunosorbent Assay), capturing ELISA, indirect ELISA, competitive ELISA and detection of porcine epidemic diarrhea viruses by colloidal gold test strips.

The invention provides a primer pair and a probe group for detecting actinobacillus pleuropneumoniae, and a kit for detecting the actinobacillus pleuropneumoniae based on a real-time fluorescent RAA method prepared by using the primer pair and the probe group, or a kit for detecting the actinobacillus pleuropneumoniae based on a colloidal gold test strip and the colloidal gold test strip. The invention further discloses a corresponding detection method. The screened primer pair and probe are used for a real-time fluorescent RAA kit for detecting serotype 1-18 porcine actinobacillus pleuropneumoniae, reaction can be completed only by 30 min under the constant temperature condition of 42 DEG C, the whole reaction process is low in instrument and equipment requirement, short in time consumption and easy to operate, the result can be visualized through naked eyes, and the method is more suitable for basic-level field diagnosis and instant detection.

The invention provides a colloidal gold test strip for detecting renal injury molecule 1 (kim-1) and a preparation method thereof, and aims to provide a bedside rapid diagnosis tool which is high in accuracy, strong in specificity, simple to operate and capable of rapidly reporting results. The test strip is formed by sequentially connecting a plastic bottom lining, a nitrocellulose membrane, a gold label pad, a sample pad and a water absorption pad, wherein a detection line and a quality control line are arranged on the nitrocellulose membrane. The detection line contains a KIM-1 monoclonal antibody, the quality control line contains a mouse anti-human IgG antibody, and the colloidal gold pad is coated with a colloidal gold labeled KIM-1 monoclonal antibody, so that a double-antibody sandwich structure is formed to specifically recognize human KIM-1. The preparation method comprises the following steps: spraying an antibody on the nitrocellulose membrane to form the detection line and the quality control line, preparing a colloidal gold antibody compound solution, and assembling each component. By optimizing the antibody concentration and a colloidal gold preparation process, the sensitivity and the stability of the test strip are ensured. The test strip is suitable for rapidly detecting kidney injury, and has important value for early diagnosis and treatment monitoring of acute and chronic kidney injury.

The invention discloses an anti-rabies virus N protein monoclonal antibody, a detection reagent and application of the anti-rabies virus N protein monoclonal antibody. The monoclonal antibody for resisting the rabies virus N protein comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No. 1; the amino acid sequence of the light chain variable region is as shown in SEQ ID No.2. The monoclonal antibody with relatively high affinity and detection sensitivity for resisting rabies virus N protein lays a foundation for research, development and popularization of colloidal gold test strips and fluorescence immunoassay test strips.

The invention relates to a rapid detection method for nucleic acid of iridovirus of Micropterus salmoides and a kit thereof, belonging to the technical field of molecular biology, the rapid detection method adopts a two-step method of RPA-Cas12a for treatment, can be used for fluorescence detection through fluorescence labeling and also can be used for detection through an immune colloidal gold test strip, and is simple and convenient to operate, rapid and sensitive, strong in specificity, good in stability and low in cost.

The invention discloses a deep learning-based colloidal gold test paper result analysis system for detecting renal injury molecular 1. The system comprises an image acquisition module, a region processing module, an effectiveness judgment module, a concentration analysis module and a result generation module, the image acquisition module is used for acquiring a visible light image of the kim-1 colloidal gold test paper, wherein the visible light image at least comprises a detection line area and a quality control line area, and carrying out gray standardization processing, contrast enhancement processing, edge sharpening processing and target area positioning processing on the visible light image; the region processing module is used for performing positioning and boundary segmentation to obtain a mask image which is consistent with the input image in size and is marked with the position of each functional region; the effectiveness judgment module is used for judging whether the kim-1 colloidal gold test paper is effective or not; the concentration analysis module is used for predicting the target substance concentration of the kim-1 colloidal gold test paper; and the result generation module is used for generating an analysis result of the kim-1 colloidal gold test paper and packaging the analysis result into a structured detection report.

The invention discloses a rapid detection kit for protein residues. The rapid detection kit comprises a labeled antibody, a coated antibody and a colloidal gold test strip. The labeled antibody is prepared by treating human intestinal epithelial cells and then immunizing a BALB / c mouse through a series of steps; and the coated antibody is prepared by treating human intestinal epithelial cells, immunizing New Zealand white rabbits and purifying in a subsequent process. The colloidal gold test strip is prepared into colloidal gold through a specific method, the labeled antibody and the colloidal gold are incubated, sprayed and dried, a sample pad is processed and then dried, impression and drying are conducted on a PVC bottom plate pasted with an NC film, and then cutting and assembling are conducted. The kit is high in detection speed and meets the instrument turnover requirement; the accuracy is high, and misjudgment is reduced; the method has the advantages that the sensitivity is as low as 100ng / mL, trace protein residues can be accurately detected, the risks of endoscopic infection and the like are reduced, and the method has obvious advantages in the aspect of protein residue detection of medical instruments and has a great application value.

The application provides a preparation method of a colloidal gold solution, a preparation method of a colloidal gold labeled protein, a colloidal gold solution and a colloidal gold labeled protein prepared by the method, a kit comprising the colloidal gold solution or the colloidal gold protein, a labeling pad for a colloidal gold test strip, a colloidal gold test strip, and use of the colloidal gold solution, the colloidal gold labeled protein, the labeling pad or the colloidal gold test strip in preparing a kit for detecting a disease marker in a biological sample. For example, the kit of the application can be used for detecting C-reactive protein by a colloidal gold method.

The present disclosure discloses a universal aptamer-based colloidal gold lateral flow test strip for detecting small-molecule substances and belongs to the fields of analytical chemistry, medicine, environment, food safety detection, nano-biosensing and the like. An AuNPs@poly-DNA probe is used for rapidly and sensitively capturing an aptamer, streptavidin sprayed in a test zone and streptavidin-biotin-DNAc sprayed in a control zone do not need to be changed, and another substance can be detected only by changing a nucleic acid chain part of the AuNPs@poly-DNA probe. A universal colloidal gold lateral flow test strip, which is rapid, sensitive and low in cost, has been developed. The test strip method for detecting small-molecule substances is simple, convenient and rapid, and can be used for detection at any time. Only a test solution is needed to be added into a sample port and the test strip is completely developed after 5 min, thus an experiment result can be observed and the detection efficiency can be greatly improved. Qualitative analysis can be conducted by naked eyes, and quantitative analysis can be conducted by a colloidal gold test strip quantitative analyzer.

The utility model discloses colloidal gold test paper for rapidly detecting trace element manganese in food, which comprises a support gasket 1, a nitrocellulose membrane 2 positioned on the support gasket 1, a Mn antibody detection line 3 coated in the middle of the nitrocellulose membrane 2 and control lines 4 respectively arranged on two sides of the detection line 3, and a water absorption pad 5 is connected above one end of the nitrocellulose membrane 2. A conjugate gasket 6 is connected above the other end of the sample pad 7 and is connected with a sample pad 7; the detection line 3 is coated with a monoclonal mouse anti-Mn antibody, the conjugate gasket 6 is coated with gold-labeled BSA-Mn and a control line conjugate, the control line 4 is coated with an anti-DNP antibody, and the control line conjugate is DNP-BSA labeled colloidal gold.

The invention discloses a feline leukemia virus p27 protein monoclonal antibody, a hybridoma cell strain and application thereof, and relates to the technical field of biology, the amino acid sequence of a heavy chain variable region of the monoclonal antibody is as shown in SEQ ID NO.1 in a sequence table, and the amino acid sequence of a light chain variable region of the monoclonal antibody is as shown in SEQ ID NO.2 in the sequence table. According to the invention, a cell fusion technology is utilized to establish a hybridoma cell strain p27-3A5 capable of secreting a p27 protein monoclonal antibody, and a specific monoclonal antibody 3A5 is obtained. And the minimum linear epitope identified by the antibody is identified, so that the establishment of subsequent preparation of a diagnostic kit such as a colloidal gold test strip is facilitated.