The invention provides an immune colloidal gold test paper card for testing quinolones, comprising a reaction film, a sample pad, a conjugate release pad, a water absorbing pad, and a back liner, wherein, the reaction film comprises a test zone coated with quinolones-carrier protein conjugate and a quality control zone coated with sheep-anti-mouse IgG; the conjugate release pad coats quinolones monoclonal antibody-colloidal gold marker. The invention also provides the method using the test paper card to test quinolones, which comprises the following steps: firstly, pre-treating sample; secondly, testing the sample with the test paper card; thirdly, analyzing test result. The invention can be used to test residual quantity of quinolones in animal derived food such as chicken, chicken liver, pork, pig liver, urine, and honey, and has the advantages of simple operation, high sensitivity, fast test, low cost, on-site supervision, and satisfaction of test requirement of a large quantity of samples.