177 results about "Enzyme protein" patented technology

Provided are a marine phospholipase C mutant and a use thereof. Compared with wild-type (WT) enzyme protein, at 40°C and under conditions where enzyme protein activity remains essentially unchanged, the enzyme protein half-life of the constructed mutant can be increased to 3.46 and 9.25 times that of the original enzyme protein, and the residual phosphorus content in degummed oil can be reduced from 551 ppm to 72 ppm, which shows a significant improvement over the final residual phosphorus content of 175 ppm for the blank control and the final residual phosphorus content of 131 ppm for the WT. These results demonstrate that the enzyme has improved thermal stability, enables oil degumming under various conditions, and is suitable for a broader range of industrial applications.

The invention discloses Talaromyces polyporus ETXG-1-1-1 as well as a culture method and application of the Talaromyces polyporus ETXG-1-1-1, the Talaromyces polyporus endophytic fungus Talaromyces polyporus endophytic fungus Talaromyces polyporus endophytic fungus Talaromyces polyporus ETXG-1-1-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is April 27, 2025, and the preservation number is CGMCC NO: 41927. The radix sophorae tonkinensis endophytic fungus talaromyces polyporus ETXG-1-1-1 is a talaromyces new species, can efficiently secrete extracellular enzymes such as cellulase and protease, can generate stable and high-color-gradation haematochrome, can effectively replace synthetic dyes and secondary metabolites to generate the effects of resisting bacteria, resisting oxidation, reducing blood sugar and the like, and can be used in the industries of textile, papermaking, food processing and the like.

The invention discloses a method for preparing antioxidant active polypeptide based on tyrosinase modified collagen, silk fibroin and mussel protein, and belongs to the technical field of bioengineering. According to the invention, high-activity tyrosinase protein is screened and prepared, and the prepared tyrosinase is used for dopa modification of antioxidant protein. The dopa modification degree of the antioxidant protein prepared by the method disclosed by the invention is remarkably improved, and the DPPH free radical scavenging power and superoxide anion free radical scavenging power of the antioxidant protein are enhanced. The method is suitable for various proteins such as collagen, silk fibroin and mussel protein, can be used in the fields of functional food, cosmetics, biological materials and the like, and has good adaptability and wide application prospects.

The invention relates to the technical field of plant genetic engineering, and particularly provides cloning of a GbGELP25D gene of gossypium barbadense and application of the GbGELP25D gene of gossypium barbadense. According to the invention, the GbGELP25D gene with a full length of 1092 bp is cloned from the sea island cotton variety Xinhai No. 7 for the first time, and the gene encodes a secretory lipase protein with a signal peptide and is positioned in an extracellular gap. Through bioinformatics analysis, phylogenetic classification, protein structure modeling and signal peptide function verification, it is clear that the gene belongs to a plant GELP family. Furthermore, a virus-induced gene silencing technology is utilized to prove that the silent GbGELP25D can obviously enhance the resistance of cotton to verticillium wilt, and the mechanism of the silent GbGELP25D is closely related to activation of ethylene synthesis and signal channels and induction of expression of disease-resistant related genes. The resistance gene provided by the invention enriches gene resources of cotton verticillium wilt resistance breeding, and has important theoretical significance and application value.

This disclosure provides improvised reagents and methods for inserting a transgene into the genome of a living host cell. Sleeping beauty (SB) transposase is used in protein form rather than as a vector. This has been made possible by using rational mutagenesis in a particular region of the SB crystal structure to develop variant SB transposase protein that is more soluble. The changes increase solubility while maintaining transposase activity, thereby adapting the variant transposase for the purpose of promoting recombinant integration of a transgene into a target cell when used in protein form rather than as a polynucleotide vector. The modified transposase is highly soluble in electroporation buffer and thermostable during storage. When introduced into a host cell, it promotes integration of a transgene into the genome of the cell in a dose-dependent manner. It is degraded within 48 hours, thereby rapidly clearing transposase activity from the host cell.

The present application relates to a kind of cytochrome P450 oxidase CYP716C52 protein, and the CYP716C52 protein coding gene, the protein can catalyze hydroxylation of maytenic acid C2 position to generate triptolide acid C, in turn participate in triptolide biosynthesis.

The application discloses a duck Riemerella anatipestifer (RA) surface adhesion factor 3-glyceraldehyde-3-phosphate dehydrogenase protein (GAPDH) conservative B cell epitope peptide, a nucleic acid molecule, a recombinant carrier and application thereof. The application inserts the conservative B cell epitope TG12 into chicken white dysentery Salmonella Peg fimbria, introduces the inert carrier bacteria, and obtains the recombinant bacteria which can functionally express and present the RaGAPDH protein surface conservative B cell epitope peptide. The expression and presentation of the conservative B cell epitope can specifically recognize and combine duck and goose RA infection serum, and the naked-eye visible agglutination reaction particles are observed, and there is no cross agglutination reaction with other pathogen infection positive serum. The agglutination detection method has the advantages of specificity and convenience, and is expected to provide a new idea and method for detection and prevention and control of RA infection.

The present application is suitable for the field of molecular biology and biotechnology, and provides a maize gibberellin 13-hydroxylase gene ZmGA13ox1 and application thereof. The maize gibberellin 13-hydroxylase gene ZmGA13ox1 has a nucleotide sequence shown as SEQ ID NO:1 in the sequence listing. The maize gibberellin 13-hydroxylase protein is encoded by the maize gibberellin 13-hydroxylase gene ZmGA13ox1, and has an amino acid sequence shown as SEQ ID NO:2 in the sequence listing. The present application provides the nucleotide sequence of the ZmGA13ox1 gene in the maize inbred line B73 and the amino acid sequence information of the protein encoded thereby, and uses the agrobacterium-mediated transformation technology to introduce a plant expression vector containing the gene into Arabidopsis thaliana, and cultivates a homozygous T3 generation transgenic Arabidopsis thaliana plant. The experimental results show that the overexpression of the ZmGA13ox1 gene in Arabidopsis thaliana has a promoting effect on the root growth.

The invention discloses 5 alpha-reductase degradation molecules as well as a preparation method and application thereof, four 5 alpha-reductase degradation molecules can realize thorough removal of zymoprotein by inducing proteasome-dependent degradation of 5 alpha-reductase instead of only inhibiting the activity of the 5 alpha-reductase, so that a more lasting treatment effect is obtained; under the condition of equal dosage, the effect of the 5 alpha-reductase degradation molecule on treating the benign prostatic hyperplasia is superior to that of the traditional inhibitor finasteride; the four kinds of 5 alpha-reductase degrading molecules can effectively degrade 5 alpha-reductase protein and remarkably inhibit development of benign prostatic hyperplasia, have good biological safety and provide new strategies and candidate drugs for treatment of diseases related to 5 alpha-reductase such as benign prostatic hyperplasia, prostatic cancer, androgenetic alopecia and acne.

The invention belongs to the technical field of biology, and provides an application of alpha-amylase protein derived from nephroplasmosis in culture of nephroplasmosis, and the alpha-amylase protein is obtained by synthesizing a gene sequence of alpha-amylase as shown in SEQ ID NO.2 after codon optimization and then transfecting the gene sequence to an expression vector. And carrying out bacterial transformation, culture, induction and crushing. The invention also provides a method for inducing the formation of the reproductive cyst of the reniform worm. It is found for the first time that alpha-amylase protein specifically induces reproductive cysts to be formed, the biomass of the nephroplasmosis is increased, the growth generation of the nephroplasmosis is shortened, and the method is suitable for species such as distended nephroplasmosis and nephroplasmosis henslouianum and fits natural ecological scenes; complex equipment is not needed, the cost is low, large-scale production is easy, the stability is high, and the method can be widely applied to soil ecological restoration, agricultural microbial resource development and basic research related to nephroplasmosis.

This invention belongs to the field of bioenzymes, specifically relating to lipase mutants, their encoding genes, expression plasmids, and applications. The lipase mutant is an amino acid sequence containing at least one mutation, either M81C or F329R, in the wild-type amino acid sequence shown in SEQ ID NO.1. This invention innovatively mutates Met at position 81 (M81C) and / or Phe at position 329 (F329R) of the Geotrichum candida Y162 lipase GCL protein sequence (wild-type amino acid sequence); this effectively improves the enzyme activity, stability, and other properties of the mutant.

This invention pertains to fusion protein mutants comprising a Prime Editing enzyme having a first amino acid sequence and a second amino acid sequence, wherein the first amino acid sequence comprises a SpCas9 H840A nickase mutant protein of SEQ ID NO:152 and the second amino acid sequence comprises a Moloney Murine Leukemia Virus reverse transcriptase protein mutant (MMLV RTase mutant), wherein the fusion protein mutant displays at least the equivalent or greater activity of a reference Prime Editing enzyme in genome editing.

The present invention provides a method for producing a baked or par-baked product, comprising the steps of: a) preparing a dough comprising a mature thermostable variant of a parent glucoamylase that is at least 70% identical to SEQ ID NO: 1, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, or SEQ ID NO: 10, added in an amount of 0.01 to 12.40 mg of enzyme protein (mgEP) per kg of flour, wherein the dough has a pH value in the range of 3.0 to 6.5; and b) baking or par-baking the dough to produce a baked or par-baked product.

Aspects of the disclosure relate to compositions and methods for treating leukodystrophies, such as Canavan Disease. The disclosure relates, in part, to nucleic acids comprising evolved nucleotide sequences encoding aspartoacylase protein. In some embodiments, the methods comprise administering the isolated nucleic acids or rAAVs encoding the aspartoacylase (ASPA) protein to a subject.

The present application relates to the field of enzymatic reaction device, specifically to an enzyme protein ultrasonic pretreatment and temperature control enzyme acceleration reaction system and operation method, which is used to solve the contradiction between enzymatic efficiency and enzyme activity and the problem of insufficient dynamic stability of reaction conditions. The system includes a reaction container, a bearing structure, an ultrasonic transducer array, an ultrasonic generator, a temperature sensor, a temperature control device, an enzymatic reaction monitoring module and a controller; the ultrasonic pretreatment improves the accessibility of the substrate, the temperature control device maintains the stability of the reaction temperature, the monitoring module collects pH and turbidity data in real time, and the controller outputs a termination signal based on the turbidity threshold. The system is mainly used in the enzymatic reaction process of enzyme protein to improve the reaction efficiency and product quality.

The utility model relates to a device for removing impure protein in crude polysaccharide of traditional Chinese medicine by utilizing immobilized protease, which comprises a protein removal reactor loaded with the immobilized protease, a dissolved oxygen tester and a constant-temperature magnetic stirrer, and a dissolved oxygen electrode of the dissolved oxygen tester is connected to the protein removal reactor. The papain immobilized on the microcrystalline cellulose is used for carrying out enzymolysis reaction on impure protein in the traditional Chinese medicine crude polysaccharide, and the device has the characteristics of simplicity in operation, mild conditions and environment friendliness. The immobilized papain is limited between the two layers of screens, so that the problem of separation of the immobilized papain in the traditional Chinese medicine crude polysaccharide solution can be avoided, and the method has the characteristics of rapidness and convenience. The immobilized papain in the device can be expanded to immobilize other proteases, such as bromelain, subtilisin, protease K and the like, on cellulose according to protein types in different traditional Chinese medicine polysaccharide solutions.

The invention discloses a preparation method of composite aerogel, which comprises the following steps: stirring and mixing a silk fibroin solution and a methanol solution of Zn (CH3COO) 2.2 H2O, and dropwise adding a methanol solution of 2-methylimidazole for reaction to form a composite system; then adding polyvinyl alcohol, dissolving at high temperature, reacting at 60 DEG C for 0.5-2 hours, repeatedly freezing and unfreezing at-80 DEG C for 3-5 times, and finally freezing and drying to obtain the composite aerogel material. According to the invention, ZIF-8 is grown on silk fibroin in situ, abundant hydrophilic groups are provided by using a beta-folding structure of the ZIF-8, an active center and a zymoprotein environment of natural carbonic anhydrase are simulated, and proton transport and hydration reaction processes are promoted; meanwhile, a multistage pore channel is constructed by combining micropores of ZIF-8 and mesopores of aerogel, so that the cooperation of CO2 adsorption and catalysis is realized. The three-dimensional aerogel structure is beneficial to high dispersion of active sites and material forming, and has a good industrial application prospect.

The invention belongs to the technical field of synthetic biology and fermentation engineering, and discloses an engineering bacterium for producing Indigoidine based on membrane vesicle engineering reinforced corynebacterium glutamicum and application of the engineering bacterium. According to the invention, the ncp1 gene of corynebacterium glutamicum is knocked out through a CRISPR-Cas12a gene editing technology, connection between cell walls and cell membranes is relieved, and a chassis strain of high-yield extracellular membrane vesicles (OMVs) is constructed; meanwhile, a recombinant expression vector is introduced, indigo synthetase BpsA is positioned and displayed on membrane vesicles by utilizing PorB anchoring protein, and activating enzyme Sfp is co-expressed. In fermentation production, a staged feeding and IPTG (isopropyl-beta-d-thiogalactoside) and Tween-80 dual induction strategy is adopted to promote thalli to express zymoprotein and release a large number of membrane vesicles at the same time. According to the method, the membrane vesicles are used as an extracellular microreactor, so that the'simultaneous synthesis and secretion 'of the indissolvable indigo is realized, the problems of cytotoxicity and metabolic inhibition caused by intracellular precipitation of the product are effectively solved, and the yield and extraction efficiency of the indigo are remarkably improved.

The invention provides a genetic engineering strain of high-yield immunosuppressant mycophenolic acid as well as a preparation method and application of the genetic engineering strain. The invention firstly provides a method for constructing the penicillium with the effect of high yield of mycophenolic acid. The method comprises the step of replacing an indigenous promoter of acyl-coenzyme A oxidase PbACOX323 protein participating in beta oxidation in peroxisome of a penicillium strain with a promoter PgpdA of 3-phosphoglycerol dehydrogenase with an amino acid sequence of SEQ ID NO: 1. According to the method disclosed by the invention, the genetically engineered bacterium with high yield of mycophenolic acid is constructed, the yield of mycophenolic acid is positively increased by replacing a promoter of acyl-coenzyme A oxidase PbACOX323, and the method has a good industrial application value.

According to the invention, beta-glycosidase which has a catalytic effect on vincoside, loganin and strictosamide is selected, and a foundation is laid for diversity and possibility of an aglycone production process. By researching the three-dimensional structure information of the Gelsemium elegans Beta-glycosidase, a zymoprotein structure information basis is provided for molecular modification of the Gelsemium elegans Beta-glycosidase, enzyme variants capable of improving substrate selectivity, thermal stability and catalytic efficiency are obtained more efficiently, and the requirements of industrial application are met; the invention provides a truncated mutant enzyme with improved enzymatic activity, and through induced expression and activity screening, it is found that the enzyme activity of the obtained mutant enzyme to loganin and strictosamide is about two times higher than that of a wild enzyme.

The invention provides an expression vector, an expression system and a preparation method of a recombinant larosidase protein, and belongs to the technical field of recombinant protein preparation, the recombinant expression vector of the larosidase protein provided by the invention comprises an initial vector and a codon-optimized human-derived larosidase coding gene; the nucleotide sequence of the human-derived larosidase coding gene is as shown in SEQ ID NO. 1; according to the recombinant expression system, Chinese hamster ovary cells are taken as host cells, and the recombinant expression vector is transfected. According to the invention, a gene sequence more suitable for expression of Chinese hamster ovary cells is obtained through codon optimization, and then transposon-mediated stable integration is carried out; high-density suspension culture and downstream purification processes are utilized to realize efficient and stable expression of the recombinant larosidase in CHO cells, and the recombinant larosidase with high yield, high activity and high purity can be obtained.

The invention discloses a soybean alpha-amylase gene-based pollen lethal module and application thereof, the soybean alpha-amylase gene-based pollen lethal module comprises a pollen specific promoter, a plastid leader peptide gene and a soybean alpha-amylase gene, and the pollen specific promoter, the plastid leader peptide gene and the soybean alpha-amylase gene are sequentially connected in a direction from 5'to 3 ', the plastid leader peptide gene and the soybean alpha-amylase gene form a fusion gene, the pollen specific promoter is a soybean GmPG031 gene promoter, the nucleotide sequence of the pollen specific promoter is as shown in SEQ ID NO: 1, the soybean alpha-amylase gene is driven by the pollen specific promoter to be directionally expressed in a male gamete, and the plastid leader peptide gene is combined to guide zymoprotein to amyloid plastid, so that the soybean alpha-amylase gene is obtained. Starch reserve in the pollen is specifically consumed, so that the pollen carrying the module is aborted due to energy failure, the mechanism realizes accurate death of the transgenic pollen, a basic element is provided for a soybean seed production technology, and a core tool is provided for large-scale creation of a male sterile line.

Provided herein are kits, compositions, systems, and methods for treating a subject with a tumor with kynurenine responsive and degrading bacteria (e.g., such that the bacteria reduces or eliminates the tumor). In certain embodiments, the kynurenine responsive bacteria comprise one or more nucleic acid sequences encoding: i) a kynurenine transcriptional regulator protein, ii) a kynurenine transporter protein, iii) a kynureninase (KynU) protein, and iv) at least one protein important or essential for growth of the bacteria expression of which is linked to a kynurenine responsive promoter (e.g., where the corresponding gene(s) has been deactivated or knocked out in the genome of the bacteria).

The present invention belongs to the field of biological detection technology, and specifically relates to a monoclonal antibody combination and application for the detection of Candida albicans enolase protein. The monoclonal antibody combination comprises monoclonal antibodies 5G7 and 5B1. The amino acid sequences of the CDR regions of monoclonal antibodies 5G7 and 5B1 are shown in SEQ ID NO.1-12. The antibody combination has high specificity and binding activity and can be used for the efficient detection of Candida albicans enolase. The colloidal gold detection test strip constructed based on the combination has good sensitivity and specificity, with a minimum detection limit of up to 500pg / ml, and does not cross-react with other common fungi. The present invention provides a reliable tool for the early diagnosis and rapid detection of invasive Candida infections, and has good clinical application prospects.

Fusion proteins and methods for conditionally fine-tuning transposase protein stability and activity for clinical applications are provided herein

Methods for determining the function and / or activity and / or potency of isohydrolase proteins are provided.

Disclosed herein are recombinant adeno-associated viral vectors expressing aspartoacylase (ASPA) protein and related uses for treating leukodystrophies.

The invention discloses an intestine-moistening and stomach-protecting wheat peptide and a preparation method and application thereof, belongs to the technical field of biological peptides, and particularly relates to a preparation method of the intestine-moistening and stomach-protecting wheat peptide, which comprises the following steps: pre-mixing wheat gluten protein with water to obtain a wheat gluten protein aqueous solution; dropwise adding a NaOH aqueous solution, centrifuging, and taking supernate; adding citric acid to adjust the pH value, standing, and taking a precipitate; dissolving the precipitate in water to obtain a protein solution, adding a compound enzyme preparation, carrying out enzymolysis to obtain a peptide solution, centrifuging, taking a supernatant to obtain a wheat peptide solution, adding mannitol, then adding glycine, and carrying out ultrafiltration and freeze drying to obtain wheat peptide; the compound enzyme preparation comprises ProteAX enzyme, Alcalase protease and EDTA (Ethylene Diamine Tetraacetic Acid). By adjusting the preparation method and matching with the special compound enzyme preparation prepared from ProteAX enzyme, Alcala enzyme protease and EDTA, the intestine moistening and stomach protecting effects of the prepared wheat peptide are improved.

The present description relates to a recombinant microorganism having production activity of pentamethylenediamine (PMDA), which comprises an E. coli Nissle-derived decarboxylase enzyme protein, and uses thereof.