247 results about "Enzyme protein" patented technology

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for detecting candida albicans enolase protein and application. And the monoclonal antibody combination comprises monoclonal antibodies 5G7 and 5B1. The amino acid sequences of CDR regions of the monoclonal antibody 5G7 and the monoclonal antibody 5B1 are as shown in SEQ ID NO.1-12. The antibody combination has high specificity and binding activity, and can be used for efficient detection of candida albicans enolase. The colloidal gold test strip constructed on the basis of the combination has good sensitivity and specificity, the lowest detection limit can reach 500pg / ml, and the colloidal gold test strip does not have cross reaction with other common fungi. The invention provides a reliable tool for early diagnosis and rapid detection of invasive candida infection, and has a good clinical application prospect.

Provided are a marine phospholipase C mutant and a use thereof. Compared with wild-type (WT) enzyme protein, at 40°C and under conditions where enzyme protein activity remains essentially unchanged, the enzyme protein half-life of the constructed mutant can be increased to 3.46 and 9.25 times that of the original enzyme protein, and the residual phosphorus content in degummed oil can be reduced from 551 ppm to 72 ppm, which shows a significant improvement over the final residual phosphorus content of 175 ppm for the blank control and the final residual phosphorus content of 131 ppm for the WT. These results demonstrate that the enzyme has improved thermal stability, enables oil degumming under various conditions, and is suitable for a broader range of industrial applications.

The utility model discloses a stirring device for industrial enzyme preparation production, which relates to the technical field of enzyme preparation production and comprises a tank body, outer channel steel for reinforcement is sleeved at the bottom of the tank body, a cover plate is hermetically connected to the top of the tank body, and a feed pipe penetrates through the cover plate. A discharging pipe communicated with the interior of the tank body is arranged on one side of the tank body, and an observation window is formed in the side wall of the tank body. The conveying rod is driven by the output shaft of the motor to rotate in the conveying pipe, fermentation liquor at the bottom of the tank body can be conveyed to the upper portion along the conveying pipe, damage of shear force to zymoprotein molecules is reduced, the fermentation liquor flows all around under the guidance of the guide plate and the arc-shaped groove, the circulation speed of the fermentation liquor in the tank body is increased, and the fermentation efficiency is improved. The cells are in full contact with nutrient substances, it can be ensured that temperature distribution in fermentation liquor is more uniform, local overheating or supercooling is avoided, and the growth speed and enzyme production efficiency of the cells are improved.

The invention discloses Talaromyces polyporus ETXG-1-1-1 as well as a culture method and application of the Talaromyces polyporus ETXG-1-1-1, the Talaromyces polyporus endophytic fungus Talaromyces polyporus endophytic fungus Talaromyces polyporus endophytic fungus Talaromyces polyporus ETXG-1-1-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is April 27, 2025, and the preservation number is CGMCC NO: 41927. The radix sophorae tonkinensis endophytic fungus talaromyces polyporus ETXG-1-1-1 is a talaromyces new species, can efficiently secrete extracellular enzymes such as cellulase and protease, can generate stable and high-color-gradation haematochrome, can effectively replace synthetic dyes and secondary metabolites to generate the effects of resisting bacteria, resisting oxidation, reducing blood sugar and the like, and can be used in the industries of textile, papermaking, food processing and the like.

The invention discloses an AxSDR (Adenosine Diphosphate Dehydrogenase Regulator) gene of an Algaea xinjiangensis source enzyme, a recombinant bacterium containing the gene and an application of the recombinant bacterium and the AxSDR gene of the Algaea xinjiangensis source enzyme. The nucleotide sequence of the enzyme AxSDR gene is as shown in SEQ ID NO. 1; the amino acid sequence of the enzyme protein coded by the enzyme AxSDR gene is SEQ ID NO. 2. The AxSDR gene is used for constructing a recombinant plasmid, and then the recombinant plasmid is introduced into E.coli BL21 (DE3) to obtain a gene recombinant bacterium containing the gene. Enzyme protein coded by the AxSDR gene or recombinant bacteria containing the gene can catalytically reduce 1500 mmol / L of m-trifluoromethyl acetophenone to prepare a chiral compound (R)-1-(3-trifluoromethyl phenyl) ethanol, conversion of a high-concentration (282g / L) substrate is achieved, the reaction lasts for 11 hours, the product yield is 99.6%, and the enantiomer excess value is 99% or above.

The invention relates to the technical field of enzyme preparations, in particular to an agricultural biological compound enzyme preparation and a preparation method thereof.The agricultural biological compound enzyme preparation is prepared from, by weight, 30-50 parts of compound enzyme, 5-8 parts of trehalose, 3-5 parts of glycerin, 15-20 parts of copolymer carrier, 5-8 parts of humic acid, 5-10 parts of bran, 3-5 parts of dipotassium phosphate and 85-124 parts of water. The compound enzyme contains various enzymes such as cellulase, lysozyme and protease, can synchronously decompose various organic substances such as cellulose, microbial cell walls and protein in soil and release nutrients such as nitrogen, phosphorus and potassium, has more comprehensive functions compared with a single enzyme preparation, and can meet the requirements of complex soil and crops in agricultural production.

The invention discloses a method for preparing antioxidant active polypeptide based on tyrosinase modified collagen, silk fibroin and mussel protein, and belongs to the technical field of bioengineering. According to the invention, high-activity tyrosinase protein is screened and prepared, and the prepared tyrosinase is used for dopa modification of antioxidant protein. The dopa modification degree of the antioxidant protein prepared by the method disclosed by the invention is remarkably improved, and the DPPH free radical scavenging power and superoxide anion free radical scavenging power of the antioxidant protein are enhanced. The method is suitable for various proteins such as collagen, silk fibroin and mussel protein, can be used in the fields of functional food, cosmetics, biological materials and the like, and has good adaptability and wide application prospects.

The invention relates to the technical field of odor-absorbing towels, in particular to a bacillus subtilis-based biological enzyme odor-absorbing towel and a preparation method thereof.The bacillus subtilis-based biological enzyme odor-absorbing towel comprises the following four layers: a surface layer, a breathable anti-reverse-osmosis non-woven fabric or nano-silver coating material, the pore diameter of the surface layer is smaller than or equal to 0.1 micron, and the surface layer is used for gas adsorption and liquid blocking; the biological enzyme filter membrane layer is formed by loading bacillus subtilis spores (greater than or equal to 108 CFU / g) and immobilized enzymes (lipase and protease) by a sodium alginate / chitosan composite hydrogel membrane; the specific surface area of the adsorption layer is larger than or equal to 1200 m < 2 > / g, the H2S adsorption capacity of the adsorption layer is larger than or equal to 200 mg / g, and the adsorption layer is used for pre-adsorbing high-concentration sulfides; the bottom layer is a microporous PE leakage-proof film, and the humidity is maintained at 60%-80% RH. According to the invention, peculiar smell molecules can be effectively degraded, liquid reverse osmosis can be prevented, and good air permeability and humidity regulation and control capability can be realized.

The invention relates to the technical field of plant genetic engineering, and particularly provides cloning of a GbGELP25D gene of gossypium barbadense and application of the GbGELP25D gene of gossypium barbadense. According to the invention, the GbGELP25D gene with a full length of 1092 bp is cloned from the sea island cotton variety Xinhai No. 7 for the first time, and the gene encodes a secretory lipase protein with a signal peptide and is positioned in an extracellular gap. Through bioinformatics analysis, phylogenetic classification, protein structure modeling and signal peptide function verification, it is clear that the gene belongs to a plant GELP family. Furthermore, a virus-induced gene silencing technology is utilized to prove that the silent GbGELP25D can obviously enhance the resistance of cotton to verticillium wilt, and the mechanism of the silent GbGELP25D is closely related to activation of ethylene synthesis and signal channels and induction of expression of disease-resistant related genes. The resistance gene provided by the invention enriches gene resources of cotton verticillium wilt resistance breeding, and has important theoretical significance and application value.

This disclosure provides improvised reagents and methods for inserting a transgene into the genome of a living host cell. Sleeping beauty (SB) transposase is used in protein form rather than as a vector. This has been made possible by using rational mutagenesis in a particular region of the SB crystal structure to develop variant SB transposase protein that is more soluble. The changes increase solubility while maintaining transposase activity, thereby adapting the variant transposase for the purpose of promoting recombinant integration of a transgene into a target cell when used in protein form rather than as a polynucleotide vector. The modified transposase is highly soluble in electroporation buffer and thermostable during storage. When introduced into a host cell, it promotes integration of a transgene into the genome of the cell in a dose-dependent manner. It is degraded within 48 hours, thereby rapidly clearing transposase activity from the host cell.

The present application relates to a kind of cytochrome P450 oxidase CYP716C52 protein, and the CYP716C52 protein coding gene, the protein can catalyze hydroxylation of maytenic acid C2 position to generate triptolide acid C, in turn participate in triptolide biosynthesis.

The application discloses a duck Riemerella anatipestifer (RA) surface adhesion factor 3-glyceraldehyde-3-phosphate dehydrogenase protein (GAPDH) conservative B cell epitope peptide, a nucleic acid molecule, a recombinant carrier and application thereof. The application inserts the conservative B cell epitope TG12 into chicken white dysentery Salmonella Peg fimbria, introduces the inert carrier bacteria, and obtains the recombinant bacteria which can functionally express and present the RaGAPDH protein surface conservative B cell epitope peptide. The expression and presentation of the conservative B cell epitope can specifically recognize and combine duck and goose RA infection serum, and the naked-eye visible agglutination reaction particles are observed, and there is no cross agglutination reaction with other pathogen infection positive serum. The agglutination detection method has the advantages of specificity and convenience, and is expected to provide a new idea and method for detection and prevention and control of RA infection.

The present application is suitable for the field of molecular biology and biotechnology, and provides a maize gibberellin 13-hydroxylase gene ZmGA13ox1 and application thereof. The maize gibberellin 13-hydroxylase gene ZmGA13ox1 has a nucleotide sequence shown as SEQ ID NO:1 in the sequence listing. The maize gibberellin 13-hydroxylase protein is encoded by the maize gibberellin 13-hydroxylase gene ZmGA13ox1, and has an amino acid sequence shown as SEQ ID NO:2 in the sequence listing. The present application provides the nucleotide sequence of the ZmGA13ox1 gene in the maize inbred line B73 and the amino acid sequence information of the protein encoded thereby, and uses the agrobacterium-mediated transformation technology to introduce a plant expression vector containing the gene into Arabidopsis thaliana, and cultivates a homozygous T3 generation transgenic Arabidopsis thaliana plant. The experimental results show that the overexpression of the ZmGA13ox1 gene in Arabidopsis thaliana has a promoting effect on the root growth.

The invention discloses 5 alpha-reductase degradation molecules as well as a preparation method and application thereof, four 5 alpha-reductase degradation molecules can realize thorough removal of zymoprotein by inducing proteasome-dependent degradation of 5 alpha-reductase instead of only inhibiting the activity of the 5 alpha-reductase, so that a more lasting treatment effect is obtained; under the condition of equal dosage, the effect of the 5 alpha-reductase degradation molecule on treating the benign prostatic hyperplasia is superior to that of the traditional inhibitor finasteride; the four kinds of 5 alpha-reductase degrading molecules can effectively degrade 5 alpha-reductase protein and remarkably inhibit development of benign prostatic hyperplasia, have good biological safety and provide new strategies and candidate drugs for treatment of diseases related to 5 alpha-reductase such as benign prostatic hyperplasia, prostatic cancer, androgenetic alopecia and acne.

The present invention provides a mutant acetyl-CoA carboxylase (ACC) protein, a nucleic acid and an application thereof, and in particular, relates to a mutant acetyl-CoA carboxylase (ACC) protein, a nucleic acid and an application thereof in plant breeding. In particular, the present invention provides a mutant acetyl-CoA carboxylase (ACC) protein, and the mutant acetyl-CoA carboxylase (ACC) protein is compared with the parent acetyl-CoA carboxylase (ACC) protein in the region corresponding to SEQ ID Mutations occur at any one or several of the following amino acid positions in the amino acid sequence shown in No. 1: 2125, 2097, 2139, 2194, 2186, 2273, 2168, 1975, 1954, 1864, 2211, 2187, 2123, and 2126; plants with the acetyl-CoA carboxylase (ACC) mutation have high herbicide resistance and have very broad application prospects in breeding herbicide-resistant plants.

Compounds and pharmaceutical compositions useful to treat cancer (e.g., lymphoma), neurodegenerative diseases, and autoimmune disorders include (1) a first nucleic acid sequence capable of binding to a transcription factor, for example, a signal transducer and activator of transcription (STAT) factor, such as STAT3, (2) a second nucleic acid sequence capable of binding a Toll-like receptor protein, for example, a CpG oligodeoxynucleotide, and (3) a ubiquitin ligase binding compound capable of binding a ubiquitin ligase protein, for example, a compound that is capable of binding a cereblon protein, such as lenalidomide, pomalidomide, or thalidomide.

The invention belongs to the technical field of biology, and provides an application of alpha-amylase protein derived from nephroplasmosis in culture of nephroplasmosis, and the alpha-amylase protein is obtained by synthesizing a gene sequence of alpha-amylase as shown in SEQ ID NO.2 after codon optimization and then transfecting the gene sequence to an expression vector. And carrying out bacterial transformation, culture, induction and crushing. The invention also provides a method for inducing the formation of the reproductive cyst of the reniform worm. It is found for the first time that alpha-amylase protein specifically induces reproductive cysts to be formed, the biomass of the nephroplasmosis is increased, the growth generation of the nephroplasmosis is shortened, and the method is suitable for species such as distended nephroplasmosis and nephroplasmosis henslouianum and fits natural ecological scenes; complex equipment is not needed, the cost is low, large-scale production is easy, the stability is high, and the method can be widely applied to soil ecological restoration, agricultural microbial resource development and basic research related to nephroplasmosis.

This invention belongs to the field of bioenzymes, specifically relating to lipase mutants, their encoding genes, expression plasmids, and applications. The lipase mutant is an amino acid sequence containing at least one mutation, either M81C or F329R, in the wild-type amino acid sequence shown in SEQ ID NO.1. This invention innovatively mutates Met at position 81 (M81C) and / or Phe at position 329 (F329R) of the Geotrichum candida Y162 lipase GCL protein sequence (wild-type amino acid sequence); this effectively improves the enzyme activity, stability, and other properties of the mutant.

This invention pertains to fusion protein mutants comprising a Prime Editing enzyme having a first amino acid sequence and a second amino acid sequence, wherein the first amino acid sequence comprises a SpCas9 H840A nickase mutant protein of SEQ ID NO:152 and the second amino acid sequence comprises a Moloney Murine Leukemia Virus reverse transcriptase protein mutant (MMLV RTase mutant), wherein the fusion protein mutant displays at least the equivalent or greater activity of a reference Prime Editing enzyme in genome editing.

The present invention provides a method for producing a baked or par-baked product, comprising the steps of: a) preparing a dough comprising a mature thermostable variant of a parent glucoamylase that is at least 70% identical to SEQ ID NO: 1, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, or SEQ ID NO: 10, added in an amount of 0.01 to 12.40 mg of enzyme protein (mgEP) per kg of flour, wherein the dough has a pH value in the range of 3.0 to 6.5; and b) baking or par-baking the dough to produce a baked or par-baked product.

Aspects of the disclosure relate to compositions and methods for treating leukodystrophies, such as Canavan Disease. The disclosure relates, in part, to nucleic acids comprising evolved nucleotide sequences encoding aspartoacylase protein. In some embodiments, the methods comprise administering the isolated nucleic acids or rAAVs encoding the aspartoacylase (ASPA) protein to a subject.

The present application relates to the field of enzymatic reaction device, specifically to an enzyme protein ultrasonic pretreatment and temperature control enzyme acceleration reaction system and operation method, which is used to solve the contradiction between enzymatic efficiency and enzyme activity and the problem of insufficient dynamic stability of reaction conditions. The system includes a reaction container, a bearing structure, an ultrasonic transducer array, an ultrasonic generator, a temperature sensor, a temperature control device, an enzymatic reaction monitoring module and a controller; the ultrasonic pretreatment improves the accessibility of the substrate, the temperature control device maintains the stability of the reaction temperature, the monitoring module collects pH and turbidity data in real time, and the controller outputs a termination signal based on the turbidity threshold. The system is mainly used in the enzymatic reaction process of enzyme protein to improve the reaction efficiency and product quality.

The utility model relates to a device for removing impure protein in crude polysaccharide of traditional Chinese medicine by utilizing immobilized protease, which comprises a protein removal reactor loaded with the immobilized protease, a dissolved oxygen tester and a constant-temperature magnetic stirrer, and a dissolved oxygen electrode of the dissolved oxygen tester is connected to the protein removal reactor. The papain immobilized on the microcrystalline cellulose is used for carrying out enzymolysis reaction on impure protein in the traditional Chinese medicine crude polysaccharide, and the device has the characteristics of simplicity in operation, mild conditions and environment friendliness. The immobilized papain is limited between the two layers of screens, so that the problem of separation of the immobilized papain in the traditional Chinese medicine crude polysaccharide solution can be avoided, and the method has the characteristics of rapidness and convenience. The immobilized papain in the device can be expanded to immobilize other proteases, such as bromelain, subtilisin, protease K and the like, on cellulose according to protein types in different traditional Chinese medicine polysaccharide solutions.

The present invention belongs to the technical field of compound purification, and relates to a method for purifying nattokinase, which includes enzymatically hydrolyzing a nattokinase fermentation broth and then purifying the obtained enzymatically hydrolyzed solution; the method for enzymatic hydrolysis includes diluting the nattokinase fermentation broth with water to a viscosity of 180-250 mPa·s, and then enzymatically hydrolyzing the obtained nattokinase dilution with an enzyme preparation into an enzymatically hydrolyzed solution. The enzyme preparation is selected from at least one of trypsin, papain, carboxypeptidase, polyglutamic acid polypeptide enzyme, and β-lactamase. The addition amount of the enzyme preparation is 0.1%-5% of the volume of the nattokinase fermentation broth. The conditions for enzymatic hydrolysis are such that the viscosity of the enzymatically hydrolyzed solution is reduced to below 100 mPa·s. Using the method provided by the present invention can not only obtain a high yield and purity of enzyme protein, but also reduce the loss of enzyme activity.

The invention discloses a preparation method of composite aerogel, which comprises the following steps: stirring and mixing a silk fibroin solution and a methanol solution of Zn (CH3COO) 2.2 H2O, and dropwise adding a methanol solution of 2-methylimidazole for reaction to form a composite system; then adding polyvinyl alcohol, dissolving at high temperature, reacting at 60 DEG C for 0.5-2 hours, repeatedly freezing and unfreezing at-80 DEG C for 3-5 times, and finally freezing and drying to obtain the composite aerogel material. According to the invention, ZIF-8 is grown on silk fibroin in situ, abundant hydrophilic groups are provided by using a beta-folding structure of the ZIF-8, an active center and a zymoprotein environment of natural carbonic anhydrase are simulated, and proton transport and hydration reaction processes are promoted; meanwhile, a multistage pore channel is constructed by combining micropores of ZIF-8 and mesopores of aerogel, so that the cooperation of CO2 adsorption and catalysis is realized. The three-dimensional aerogel structure is beneficial to high dispersion of active sites and material forming, and has a good industrial application prospect.

The invention belongs to the technical field of synthetic biology and fermentation engineering, and discloses an engineering bacterium for producing Indigoidine based on membrane vesicle engineering reinforced corynebacterium glutamicum and application of the engineering bacterium. According to the invention, the ncp1 gene of corynebacterium glutamicum is knocked out through a CRISPR-Cas12a gene editing technology, connection between cell walls and cell membranes is relieved, and a chassis strain of high-yield extracellular membrane vesicles (OMVs) is constructed; meanwhile, a recombinant expression vector is introduced, indigo synthetase BpsA is positioned and displayed on membrane vesicles by utilizing PorB anchoring protein, and activating enzyme Sfp is co-expressed. In fermentation production, a staged feeding and IPTG (isopropyl-beta-d-thiogalactoside) and Tween-80 dual induction strategy is adopted to promote thalli to express zymoprotein and release a large number of membrane vesicles at the same time. According to the method, the membrane vesicles are used as an extracellular microreactor, so that the'simultaneous synthesis and secretion 'of the indissolvable indigo is realized, the problems of cytotoxicity and metabolic inhibition caused by intracellular precipitation of the product are effectively solved, and the yield and extraction efficiency of the indigo are remarkably improved.

The invention discloses a dipeptide modified polymer and application thereof in intracellular delivery of protein. The screened polymer and protein form a compound and are subjected to phase transformation, solute in a liquid phase is transformed into solid-phase particles insoluble in water, early release of the target protein is reduced, and delivery of the protein in the presence of serum is achieved. The protein intracellular delivery method provided by the invention has relatively high intracellular delivery efficiency in the presence of serum-free protein and serum, and the delivery efficiency is higher than that of a commercial protein transfection reagent PULSin; after beta-Gal and other enzyme proteins are delivered into cells, the activity can be recovered, a catalytic substrate is converted into a monitorable product, and the amount of the delivered and active recovered protein is less affected by serum protein in incubation conditions; in addition, the material has low cytotoxicity, the cell activity is higher than 90% under the experimental condition of protein delivery, and the material has good biocompatibility.

The invention provides a genetic engineering strain of high-yield immunosuppressant mycophenolic acid as well as a preparation method and application of the genetic engineering strain. The invention firstly provides a method for constructing the penicillium with the effect of high yield of mycophenolic acid. The method comprises the step of replacing an indigenous promoter of acyl-coenzyme A oxidase PbACOX323 protein participating in beta oxidation in peroxisome of a penicillium strain with a promoter PgpdA of 3-phosphoglycerol dehydrogenase with an amino acid sequence of SEQ ID NO: 1. According to the method disclosed by the invention, the genetically engineered bacterium with high yield of mycophenolic acid is constructed, the yield of mycophenolic acid is positively increased by replacing a promoter of acyl-coenzyme A oxidase PbACOX323, and the method has a good industrial application value.

According to the invention, beta-glycosidase which has a catalytic effect on vincoside, loganin and strictosamide is selected, and a foundation is laid for diversity and possibility of an aglycone production process. By researching the three-dimensional structure information of the Gelsemium elegans Beta-glycosidase, a zymoprotein structure information basis is provided for molecular modification of the Gelsemium elegans Beta-glycosidase, enzyme variants capable of improving substrate selectivity, thermal stability and catalytic efficiency are obtained more efficiently, and the requirements of industrial application are met; the invention provides a truncated mutant enzyme with improved enzymatic activity, and through induced expression and activity screening, it is found that the enzyme activity of the obtained mutant enzyme to loganin and strictosamide is about two times higher than that of a wild enzyme.