305 results about "Enzyme protein" patented technology

Disclosed herein are methods, compositions, and kits for determining the presence or absence of a target polypeptide (e.g., a polypeptide involved in nitrogen fixation) in a sample (e.g., a sample of soil from the rhizosphere of a non-leguminous plant).

The invention discloses a composite microbial enzyme preparation and a preparation method thereof, and belongs to the technical field of bioplastic degradation. The enzyme preparation is synergistically constructed through a temperature-sensitive MOFs / Schiff base network composite carrier and black phosphorus quantum dots, FeO (at) ZIF-8 is taken as a core, and PNIPAM is grafted on the surface to form temperature-responsive MOFs; constructing a three-dimensional network carrier through Schiff base crosslinking of the CMC and the CNF; the complex enzyme is loaded on MOFs pore channels and then is embedded in a carrier network together with the black phosphorus quantum dots. The temperature-sensitive MOFs open pore channels to release enzyme when the compost is heated, and the Schiff base network dissociates secondary released enzyme under an acidic condition; the black phosphorus quantum dots promote enzyme activity and strengthen carrier response through a photothermal effect; the compound enzyme contains cutinase, alkaline lipase, neutral lipase, alpha-amylase, saccharifying enzyme, cellulase, laccase and protease, and can synergistically degrade PLA, PBAT and starch-based plastic. According to the invention, dual environment response controlled release, photo-thermal synergistic catalysis and synergistic degradation of the enzyme preparation are realized, and the plastic degradation period is shortened under the composting condition.

The invention relates to the technical field of microorganisms, in particular to recombinant pichia pastoris for expressing plastic degrading enzyme LCCICCG as well as a construction method and application of the recombinant pichia pastoris. According to the invention, pichia pastoris is taken as a host cell, the coding gene lccICCG of the plastic degrading enzyme is heterologously expressed, and single-copy or multi-copy recombinant pichia pastoris is constructed. By optimizing culture conditions, the expression quantity and biomass of the plastic degrading enzyme protein are remarkably improved; by optimizing a fermentation strategy, high-density fermentation production of the LCCICCG protein in a 5L fermentation tank is realized, and a technical basis is provided for realizing large-scale industrial production of the protein. The improvements not only improve the production efficiency and reduce the cost of industrial production, but also provide powerful support for commercial application of the plastic depolymerases.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody combination for detecting candida albicans enolase protein and application. And the monoclonal antibody combination comprises monoclonal antibodies 5G7 and 5B1. The amino acid sequences of CDR regions of the monoclonal antibody 5G7 and the monoclonal antibody 5B1 are as shown in SEQ ID NO.1-12. The antibody combination has high specificity and binding activity, and can be used for efficient detection of candida albicans enolase. The colloidal gold test strip constructed on the basis of the combination has good sensitivity and specificity, the lowest detection limit can reach 500pg / ml, and the colloidal gold test strip does not have cross reaction with other common fungi. The invention provides a reliable tool for early diagnosis and rapid detection of invasive candida infection, and has a good clinical application prospect.

The invention discloses a method for preparing immobilized lipase Janus particles by an emulsion template one-step method based on a zymoprotein directional adsorption strategy and application of the immobilized lipase Janus particles in a Pickering emulsion catalytic system. The natural hydrophilicity of lipase protein is utilized to drive the lipase protein to form directional adsorption on the surfaces of hydrophobic particles, and the Janus particles with hydrophilic and hydrophobic characteristics are constructed. Firstly, an organic phase containing hydrophobic particles and a water phase containing lipase are oscillated to form an emulsion, so that the lipase in the water phase is accurately adsorbed on the single side of the surface of the particles, and directional connection of zymoprotein and a hydrophobic matrix is realized. Afterwards, the Janus particles are used for constructing a Pickering emulsion system, and a microreactor is built, so that the catalytic efficiency of lipase in hydrolysis, ester synthesis and transesterification reaction is improved. The method is easy to operate, efficient and stable enzyme immobilization can be achieved, the obtained immobilized lipase Janus particles are remarkably superior to free enzyme in biological catalytic activity and cyclic utilization performance, and the application value of lipase in industrial large-scale production is remarkably improved.

Provided are a marine phospholipase C mutant and a use thereof. Compared with wild-type (WT) enzyme protein, at 40°C and under conditions where enzyme protein activity remains essentially unchanged, the enzyme protein half-life of the constructed mutant can be increased to 3.46 and 9.25 times that of the original enzyme protein, and the residual phosphorus content in degummed oil can be reduced from 551 ppm to 72 ppm, which shows a significant improvement over the final residual phosphorus content of 175 ppm for the blank control and the final residual phosphorus content of 131 ppm for the WT. These results demonstrate that the enzyme has improved thermal stability, enables oil degumming under various conditions, and is suitable for a broader range of industrial applications.

The utility model discloses a stirring device for industrial enzyme preparation production, which relates to the technical field of enzyme preparation production and comprises a tank body, outer channel steel for reinforcement is sleeved at the bottom of the tank body, a cover plate is hermetically connected to the top of the tank body, and a feed pipe penetrates through the cover plate. A discharging pipe communicated with the interior of the tank body is arranged on one side of the tank body, and an observation window is formed in the side wall of the tank body. The conveying rod is driven by the output shaft of the motor to rotate in the conveying pipe, fermentation liquor at the bottom of the tank body can be conveyed to the upper portion along the conveying pipe, damage of shear force to zymoprotein molecules is reduced, the fermentation liquor flows all around under the guidance of the guide plate and the arc-shaped groove, the circulation speed of the fermentation liquor in the tank body is increased, and the fermentation efficiency is improved. The cells are in full contact with nutrient substances, it can be ensured that temperature distribution in fermentation liquor is more uniform, local overheating or supercooling is avoided, and the growth speed and enzyme production efficiency of the cells are improved.

The invention belongs to the technical field of biology, and relates to zymoprotein, in particular to recombinant arthrobacter globiforme uricase and application thereof. The recombinant arthrobacter globiforme uricase has single-site amino acid mutation, amino acids are located in an active region and an inactive region of the uricase, and the amino acid sequence of the recombinant arthrobacter globiforme uricase is shown as any one of SEQ ID NO: 1-8. Compared with wild uricase, the mutant uricase formed after amino acid is introduced into uricase at a fixed point has higher activity, and the use amount of uricase can be more effectively reduced under the condition that the content of uric acid is effectively controlled.

This disclosure provides improved reagents and methods for inserting a transgene into the genome of a living host cell. Sleeping beauty (SB) transposase is used in protein form rather than as a vector. This has been made possible by using rational mutagenesis in a particular region of the SB crystal structure to develop variant SB transposase protein that is more soluble. The changes increase solubility while maintaining transposase activity, thereby adapting the variant transposase for the purpose of promoting recombinant integration of a transgene into a target cell when used in protein form rather than as a polynucleotide vector. The modified transposase is highly soluble in electroporation buffer and thermostable during storage. When introduced into a host cell, it promotes integration of a transgene into the genome of the cell in a dose-dependent manner. It is degraded within 48 hours, thereby rapidly clearing transposase activity from the host cell.

Provided herein are compounds, preferably compounds inhibiting tyrosine kinase enzymatic activity of a protein selected from Abelson protein (ABL1), Abelson-related protein (ABL2), or a chimeric protein BCR-ABL1, compositions thereof, and methods of their preparation, and methods of inhibiting tyrosine kinase enzymatic activity of a protein selected from Abelson protein (ABL1), Abelson-related protein (ABL2), or a chimeric protein BCR-ABL1, and methods for treating diseases wherein modulation of BCR-ABL1 activity prevents, inhibits, or ameliorates the pathology and / or symptomology of the disease. Intermediates such as compounds of Formula (S23), which are useful in the synthesis of compounds inhibiting tyrosine kinase enzymatic activity of a protein selected from Abelson protein (ABL1), Abelson-related protein (ABL2), or a chimeric protein BCR-ABL1, are also provided.

The invention discloses Talaromyces polyporus ETXG-1-1-1 as well as a culture method and application of the Talaromyces polyporus ETXG-1-1-1, the Talaromyces polyporus endophytic fungus Talaromyces polyporus endophytic fungus Talaromyces polyporus endophytic fungus Talaromyces polyporus ETXG-1-1-1 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation date is April 27, 2025, and the preservation number is CGMCC NO: 41927. The radix sophorae tonkinensis endophytic fungus talaromyces polyporus ETXG-1-1-1 is a talaromyces new species, can efficiently secrete extracellular enzymes such as cellulase and protease, can generate stable and high-color-gradation haematochrome, can effectively replace synthetic dyes and secondary metabolites to generate the effects of resisting bacteria, resisting oxidation, reducing blood sugar and the like, and can be used in the industries of textile, papermaking, food processing and the like.

The invention discloses an AxSDR (Adenosine Diphosphate Dehydrogenase Regulator) gene of an Algaea xinjiangensis source enzyme, a recombinant bacterium containing the gene and an application of the recombinant bacterium and the AxSDR gene of the Algaea xinjiangensis source enzyme. The nucleotide sequence of the enzyme AxSDR gene is as shown in SEQ ID NO. 1; the amino acid sequence of the enzyme protein coded by the enzyme AxSDR gene is SEQ ID NO. 2. The AxSDR gene is used for constructing a recombinant plasmid, and then the recombinant plasmid is introduced into E.coli BL21 (DE3) to obtain a gene recombinant bacterium containing the gene. Enzyme protein coded by the AxSDR gene or recombinant bacteria containing the gene can catalytically reduce 1500 mmol / L of m-trifluoromethyl acetophenone to prepare a chiral compound (R)-1-(3-trifluoromethyl phenyl) ethanol, conversion of a high-concentration (282g / L) substrate is achieved, the reaction lasts for 11 hours, the product yield is 99.6%, and the enantiomer excess value is 99% or above.

The invention relates to the technical field of enzyme preparations, in particular to an agricultural biological compound enzyme preparation and a preparation method thereof.The agricultural biological compound enzyme preparation is prepared from, by weight, 30-50 parts of compound enzyme, 5-8 parts of trehalose, 3-5 parts of glycerin, 15-20 parts of copolymer carrier, 5-8 parts of humic acid, 5-10 parts of bran, 3-5 parts of dipotassium phosphate and 85-124 parts of water. The compound enzyme contains various enzymes such as cellulase, lysozyme and protease, can synchronously decompose various organic substances such as cellulose, microbial cell walls and protein in soil and release nutrients such as nitrogen, phosphorus and potassium, has more comprehensive functions compared with a single enzyme preparation, and can meet the requirements of complex soil and crops in agricultural production.

The invention discloses a method for preparing antioxidant active polypeptide based on tyrosinase modified collagen, silk fibroin and mussel protein, and belongs to the technical field of bioengineering. According to the invention, high-activity tyrosinase protein is screened and prepared, and the prepared tyrosinase is used for dopa modification of antioxidant protein. The dopa modification degree of the antioxidant protein prepared by the method disclosed by the invention is remarkably improved, and the DPPH free radical scavenging power and superoxide anion free radical scavenging power of the antioxidant protein are enhanced. The method is suitable for various proteins such as collagen, silk fibroin and mussel protein, can be used in the fields of functional food, cosmetics, biological materials and the like, and has good adaptability and wide application prospects.

The invention relates to the technical field of odor-absorbing towels, in particular to a bacillus subtilis-based biological enzyme odor-absorbing towel and a preparation method thereof.The bacillus subtilis-based biological enzyme odor-absorbing towel comprises the following four layers: a surface layer, a breathable anti-reverse-osmosis non-woven fabric or nano-silver coating material, the pore diameter of the surface layer is smaller than or equal to 0.1 micron, and the surface layer is used for gas adsorption and liquid blocking; the biological enzyme filter membrane layer is formed by loading bacillus subtilis spores (greater than or equal to 108 CFU / g) and immobilized enzymes (lipase and protease) by a sodium alginate / chitosan composite hydrogel membrane; the specific surface area of the adsorption layer is larger than or equal to 1200 m < 2 > / g, the H2S adsorption capacity of the adsorption layer is larger than or equal to 200 mg / g, and the adsorption layer is used for pre-adsorbing high-concentration sulfides; the bottom layer is a microporous PE leakage-proof film, and the humidity is maintained at 60%-80% RH. According to the invention, peculiar smell molecules can be effectively degraded, liquid reverse osmosis can be prevented, and good air permeability and humidity regulation and control capability can be realized.

The invention belongs to the field of enzyme engineering and agricultural waste conversion, and particularly relates to a preparation method of an acid-resistant high-activity keratinase preparation and application of the acid-resistant high-activity keratinase preparation in feather meal fermented feed. According to the invention, acid resistance design is carried out based on cotG-KERQ7, and the acid stability and activity of the keratinase mutant are both improved through a combined mutation strategy of three rounds of base catalytic residue replacement in a catalytic center, surface unstable positive charge amino acid replacement and flexible region introduction. By making the zymoprotein surface of serine keratinase more suitable for an acidic environment, the volume of a substrate binding cavity of protease in the acidic environment is increased, and the substrate can be better accepted and catalyzed, so that the acid stability and activity are effectively improved; finally, the acid-resistant and high-activity keratinase preparation B.subtilis cotG-KERQ7-M2 is obtained, and reference is provided for subsequent industrialized and fermented feed preparation for green conversion of feather waste into acid-soluble protein.

The invention discloses a protein variant combination for improving the immune function of iNKT cells and application, and relates to the technical field of tumor immunity. Comprising a protein variant combination consisting of a protein variant ANT A3, a protein variant GLUT3G1, a protein variant LDHB B2, a protein variant PC P4 and a protein variant PCK1K2; according to the invention, gene sequences of the five protein variants are simultaneously embedded into an iNKT cell genome in a fixed-point manner for the first time to form a metabolic reconstitution MetaR-iNKT cell, and it is found that when the MetaR-iNKT cell and an ATP mitochondrial transporter protein inhibitor PENAO are combined for use, lung cancer solid tumors can be synergistically and better killed.

The invention relates to the technical field of plant genetic engineering, and particularly provides cloning of a GbGELP25D gene of gossypium barbadense and application of the GbGELP25D gene of gossypium barbadense. According to the invention, the GbGELP25D gene with a full length of 1092 bp is cloned from the sea island cotton variety Xinhai No. 7 for the first time, and the gene encodes a secretory lipase protein with a signal peptide and is positioned in an extracellular gap. Through bioinformatics analysis, phylogenetic classification, protein structure modeling and signal peptide function verification, it is clear that the gene belongs to a plant GELP family. Furthermore, a virus-induced gene silencing technology is utilized to prove that the silent GbGELP25D can obviously enhance the resistance of cotton to verticillium wilt, and the mechanism of the silent GbGELP25D is closely related to activation of ethylene synthesis and signal channels and induction of expression of disease-resistant related genes. The resistance gene provided by the invention enriches gene resources of cotton verticillium wilt resistance breeding, and has important theoretical significance and application value.

This disclosure provides improvised reagents and methods for inserting a transgene into the genome of a living host cell. Sleeping beauty (SB) transposase is used in protein form rather than as a vector. This has been made possible by using rational mutagenesis in a particular region of the SB crystal structure to develop variant SB transposase protein that is more soluble. The changes increase solubility while maintaining transposase activity, thereby adapting the variant transposase for the purpose of promoting recombinant integration of a transgene into a target cell when used in protein form rather than as a polynucleotide vector. The modified transposase is highly soluble in electroporation buffer and thermostable during storage. When introduced into a host cell, it promotes integration of a transgene into the genome of the cell in a dose-dependent manner. It is degraded within 48 hours, thereby rapidly clearing transposase activity from the host cell.

The present application relates to a kind of cytochrome P450 oxidase CYP716C52 protein, and the CYP716C52 protein coding gene, the protein can catalyze hydroxylation of maytenic acid C2 position to generate triptolide acid C, in turn participate in triptolide biosynthesis.

The application discloses a duck Riemerella anatipestifer (RA) surface adhesion factor 3-glyceraldehyde-3-phosphate dehydrogenase protein (GAPDH) conservative B cell epitope peptide, a nucleic acid molecule, a recombinant carrier and application thereof. The application inserts the conservative B cell epitope TG12 into chicken white dysentery Salmonella Peg fimbria, introduces the inert carrier bacteria, and obtains the recombinant bacteria which can functionally express and present the RaGAPDH protein surface conservative B cell epitope peptide. The expression and presentation of the conservative B cell epitope can specifically recognize and combine duck and goose RA infection serum, and the naked-eye visible agglutination reaction particles are observed, and there is no cross agglutination reaction with other pathogen infection positive serum. The agglutination detection method has the advantages of specificity and convenience, and is expected to provide a new idea and method for detection and prevention and control of RA infection.

The invention provides a compound which is shown in a formula I and has a binding effect with E3 ligase protein BIRC7, and application of the compound in preparation of a medicine for treating cell abnormal proliferation diseases. The invention also provides an intermediate compound in the synthesis process of the difunctional compound for targeting protein degradation. # imgabs0 #

The present application is suitable for the field of molecular biology and biotechnology, and provides a maize gibberellin 13-hydroxylase gene ZmGA13ox1 and application thereof. The maize gibberellin 13-hydroxylase gene ZmGA13ox1 has a nucleotide sequence shown as SEQ ID NO:1 in the sequence listing. The maize gibberellin 13-hydroxylase protein is encoded by the maize gibberellin 13-hydroxylase gene ZmGA13ox1, and has an amino acid sequence shown as SEQ ID NO:2 in the sequence listing. The present application provides the nucleotide sequence of the ZmGA13ox1 gene in the maize inbred line B73 and the amino acid sequence information of the protein encoded thereby, and uses the agrobacterium-mediated transformation technology to introduce a plant expression vector containing the gene into Arabidopsis thaliana, and cultivates a homozygous T3 generation transgenic Arabidopsis thaliana plant. The experimental results show that the overexpression of the ZmGA13ox1 gene in Arabidopsis thaliana has a promoting effect on the root growth.

The invention discloses 5 alpha-reductase degradation molecules as well as a preparation method and application thereof, four 5 alpha-reductase degradation molecules can realize thorough removal of zymoprotein by inducing proteasome-dependent degradation of 5 alpha-reductase instead of only inhibiting the activity of the 5 alpha-reductase, so that a more lasting treatment effect is obtained; under the condition of equal dosage, the effect of the 5 alpha-reductase degradation molecule on treating the benign prostatic hyperplasia is superior to that of the traditional inhibitor finasteride; the four kinds of 5 alpha-reductase degrading molecules can effectively degrade 5 alpha-reductase protein and remarkably inhibit development of benign prostatic hyperplasia, have good biological safety and provide new strategies and candidate drugs for treatment of diseases related to 5 alpha-reductase such as benign prostatic hyperplasia, prostatic cancer, androgenetic alopecia and acne.

The present invention provides a mutant acetyl-CoA carboxylase (ACC) protein, a nucleic acid and an application thereof, and in particular, relates to a mutant acetyl-CoA carboxylase (ACC) protein, a nucleic acid and an application thereof in plant breeding. In particular, the present invention provides a mutant acetyl-CoA carboxylase (ACC) protein, and the mutant acetyl-CoA carboxylase (ACC) protein is compared with the parent acetyl-CoA carboxylase (ACC) protein in the region corresponding to SEQ ID Mutations occur at any one or several of the following amino acid positions in the amino acid sequence shown in No. 1: 2125, 2097, 2139, 2194, 2186, 2273, 2168, 1975, 1954, 1864, 2211, 2187, 2123, and 2126; plants with the acetyl-CoA carboxylase (ACC) mutation have high herbicide resistance and have very broad application prospects in breeding herbicide-resistant plants.

Compounds and pharmaceutical compositions useful to treat cancer (e.g., lymphoma), neurodegenerative diseases, and autoimmune disorders include (1) a first nucleic acid sequence capable of binding to a transcription factor, for example, a signal transducer and activator of transcription (STAT) factor, such as STAT3, (2) a second nucleic acid sequence capable of binding a Toll-like receptor protein, for example, a CpG oligodeoxynucleotide, and (3) a ubiquitin ligase binding compound capable of binding a ubiquitin ligase protein, for example, a compound that is capable of binding a cereblon protein, such as lenalidomide, pomalidomide, or thalidomide.

The invention relates to the technical field of enzyme synthesis, and discloses a method for synthesizing coenzyme A by combining magnetic immobilized enzyme with weak electric field, comprising the following steps: S1, bacterial seed culture; S2, fermentation culture; S3, bacterial collection and preservation; S4, preparation of magnetic immobilized carrier and immobilization of intracellular enzyme protein of Brevibacterium ammoniagenes; S5, synthesis of coenzyme A by magnetic immobilized coenzyme A synthetase. Coenzyme A is synthesized by combining magnetic immobilized enzyme with weak electric field, with high unit titer, short synthesis time, reduced oxidation of reduced coenzyme A, and no need to add mercaptoethanol for reduction in the later stage. Brevibacterium ammoniagenes is broken to free several enzyme proteins required for coenzyme A synthesis, and the purified coenzyme A synthetase is immobilized by using activated hydroxy resin carrier, with mild immobilization conditions, high protein fixation rate, less enzyme inactivation, good stability of magnetic immobilized enzyme, and one-time catalysis of pantothenic acid, adenosine triphosphate and cysteine ​​to synthesize coenzyme A at a fast speed.

The invention belongs to the technical field of biology, and provides an application of alpha-amylase protein derived from nephroplasmosis in culture of nephroplasmosis, and the alpha-amylase protein is obtained by synthesizing a gene sequence of alpha-amylase as shown in SEQ ID NO.2 after codon optimization and then transfecting the gene sequence to an expression vector. And carrying out bacterial transformation, culture, induction and crushing. The invention also provides a method for inducing the formation of the reproductive cyst of the reniform worm. It is found for the first time that alpha-amylase protein specifically induces reproductive cysts to be formed, the biomass of the nephroplasmosis is increased, the growth generation of the nephroplasmosis is shortened, and the method is suitable for species such as distended nephroplasmosis and nephroplasmosis henslouianum and fits natural ecological scenes; complex equipment is not needed, the cost is low, large-scale production is easy, the stability is high, and the method can be widely applied to soil ecological restoration, agricultural microbial resource development and basic research related to nephroplasmosis.

This invention belongs to the field of bioenzymes, specifically relating to lipase mutants, their encoding genes, expression plasmids, and applications. The lipase mutant is an amino acid sequence containing at least one mutation, either M81C or F329R, in the wild-type amino acid sequence shown in SEQ ID NO.1. This invention innovatively mutates Met at position 81 (M81C) and / or Phe at position 329 (F329R) of the Geotrichum candida Y162 lipase GCL protein sequence (wild-type amino acid sequence); this effectively improves the enzyme activity, stability, and other properties of the mutant.