139 results about "Molecular diagnostic techniques" patented technology

The invention belongs to the technical field of molecular diagnosis, and particularly discloses a high-risk HPV16 rapid detection system based on RPA-CRISPR / Cas12a, which is prepared by the following steps: (1) mixing a nucleic acid lysis solution containing Tris (pH = 8.0), NaHCO3, polyvinylpyrrolidone and Tween-20 with Proteinase K to treat a sample, and performing pyrolysis at room temperature for 5 minutes to obtain a nucleic acid template; (2) an RPA reaction system contains an RNA inhibitor, a reverse transcriptase and an RPA reaction essential enzyme, and after the template and the starting agent in the step (1) are added, RPA amplification is carried out at 37 DEG C; (3) dropping the reaction liquid on a colloidal gold test strip subjected to position exchange and transformation of a sample pad and a water absorption pad, and carrying out result interpretation; and (4) adding the RPA product into a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) reaction system containing the LbCas12a protein, the specific gRNA and the probe. According to the scheme, a rapid, high-sensitivity and cross-reaction-free detection system of the HPV16 is realized by optimizing a nucleic acid lysis solution formula, designing a specific primer and gRNA and modifying a test strip for detection.

The invention discloses an early diagnosis marker for B-cell lymphoma, a cross-species screening method based on lamprey and application, and relates to the technical field of molecular diagnosis. In the prior art, tissue biopsy is strong in invasiveness, and a traditional marker is low in early detection rate; in order to solve the problems that a large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large-scale large- When the expression quantity of the gene in a sample is greater than or equal to 1.8 times of that of a normal B cell (HMY2. CIR), the lymphoma is judged to be positive, and the minimally invasive early diagnosis efficiency is remarkably improved.

The invention discloses a nucleic acid composition for detecting fungi as well as a kit and a detection method thereof, and relates to the technical field of molecular diagnosis. The embodiment of the invention provides a group of nucleic acid compositions, the compositions comprise primer pairs 1-5, the compositions can realize synchronous detection of aspergillus, cryptococcus, pneumocystis yarrowii and mucor on the basis of a real-time fluorescent quantitative PCR technology, and the nucleic acid compositions have the remarkable advantages of short detection time, simplicity and convenience in operation, high sensitivity, strong specificity and the like.

The invention discloses a bubble self-stratification digital detection method and system for miRNA quantification of extracellular vesicles, and belongs to the technical field of biomedical engineering and molecular diagnosis. The technical problem to be solved is to provide a simple and sensitive tumor extracellular vesicle miRNA detection method capable of simultaneously detecting various miRNAs. According to the scheme, a to-be-detected sample and immunocapture bubbles coupled with an anti-EpCAM antibody are incubated to enrich tumor extracellular vesicles, a to-be-detected miRNA sample is obtained through in-situ ultrasonic lysis, then the to-be-detected miRNA sample, a fluorescence / DNA double-coding magnetic bead conjugate and the like are used for constructing an enzyme digestion system for enzyme digestion, multifunctional click glass microbubbles are added to capture unreacted magnetic beads, standing is conducted, self-stratification is conducted, bottom magnetic beads are collected, and the EpCAM antibody is obtained. After fluorescence imaging, an image is input into the AI automatic fluorescence counting and decoding module for counting to obtain a result. The method is used for detecting miR-21 and miR-155 in tumor extracellular vesicles, and is suitable for noninvasive early diagnosis of tumors.

The invention relates to the technical field of molecular diagnosis, in particular to a high-sensitivity peripheral blood free DNA magnetic bead capture system, a methylation transformation method kit and application. The kit is composed of a lysis solution, a protease K diluent, a composite conversion reagent, a washing solution, a scavenger, an eluent and combined magnetic beads. According to the high-sensitivity peripheral blood free DNA magnetic bead capture system and the methylation transformation method kit, the detection limit of early-stage lung cancer ctDNA reaches 0.005% AF, the I-stage cancer ctDNA detection rate is increased to 93% from the industry average 40%, the sample size is reduced to 4 mL from the conventional 10 mL, the batch-to-batch difference of the kit is small, and the kit has very high application value clinically.

The invention relates to the technical field of microfluidics and molecular diagnosis, and discloses a digital micro-fluidic chip for preparing an NGS methylation library. Comprising a bearing substrate, an electrode array used for electrowetting driving is formed on the upper surface of the bearing substrate to serve as a liquid drop conveying channel, the bearing substrate is divided into a plurality of functional areas which are sequentially arranged from the first side to the second side, the areas are communicated with one another through the liquid drop conveying channel, and micro-liquid drops are transferred and distributed among the areas. The upper cover is arranged opposite to the bearing substrate, a micro-droplet containing cavity is defined between the upper cover and the bearing substrate through a spacing frame arranged around the electrode array, and the upper cover is provided with a plurality of liquid inlet and outlet holes corresponding to the functional areas respectively. The chip is driven by the electrode array, so that micro-droplets entering the reagent sample introduction area and the sample introduction area through the liquid inlet and outlet holes are sequentially conveyed to the reaction area, and the micro-droplets after reaction are transferred to the taking-out area and are output through the liquid inlet and outlet holes, so that at least part of steps in the NGS methylation library preparation process are completed.

The invention discloses a preparation method and application of carassius auratus rahn aquatic OmpA gene cloning and prokaryotic expression as well as a polyclonal antibody. The preparation method comprises the following steps: culture and DNA amplification of rahn aquatic, design of an OmpA primer, gene cloning and prokaryotic expression, back multi-point subcutaneous injection of an immune rabbit, heart blood sampling and preparation of the polyclonal antibody. The invention further provides OmpA protein prokaryotic expression and protein purification, preparation of OmpA polyclonal antiserum and application of fish bodies. According to the invention, a rahnella aquatilis OmpA gene is cloned, a specific Hind III and EcoR1 double-enzyme digestion primer sequence OmpA-1F / OmpA-1R is designed, a prokaryotic expression vector pET32a-OmpA is constructed, high-immunogenicity OmpA protein is obtained through purification, OmpA specific multi-antibody serum is prepared, and the OmpA specific multi-antibody serum can be used for preparing the multi-antibody serum. The OmpA polyclonal antibody can be used for detecting and positioning the expression application of rahnella aquatica OmpA protein in crucian carp bodies through bidirectional agar diffusion, an immunoblotting method and an immunohistochemical method, and provides a reference basis for research and development and application of rahnella aquatica subunit vaccines and a molecular diagnosis technology as well as immune prevention and control of infectious diseases of the rahnella aquatica subunit vaccines and the molecular diagnosis technology.

The invention discloses a preparation method of an FLT3-ITD specific hairpin primer, a detection system and application of the FLT3-ITD specific hairpin primer, and belongs to the technical field of molecular diagnosis. The 3 '-end of the hairpin primer is located at the upstream 4-12bp position of the FLT3-ITD junction point, the 5'-end of the hairpin primer extends to the downstream region of the junction point, a section of double-chain stem region exists at the 5 '-end of the hairpin primer, and the sequence of the double-chain stem region is completely complementary with the sequence of the 3'-end of the primer, so that the hairpin primer spontaneously forms a hairpin structure; the primer is used for specific detection of FLT3-ITD mutation. Through the mode, the detection sensitivity of FLT3-ITD mutation is as low as 0.001%, and the FLT3-ITD mutation detection kit can be used for clinical AML MRD detection to assist clinical precise diagnosis and treatment.

The present application relates to the technical field of molecular diagnosis, and in particular to a composition for visual detection of glanders based on an RPA-LbCas12a system and application thereof.The present application provides a composition for detecting Burkholderia mallei, which comprises RPA primers, crRNA, LbCas12a protease and ssDNA fluorescent probes; the nucleotide sequence of the RPA primers is shown in SEQ ID NO: 5 and SEQ ID NO: 6; and the nucleotide sequence of the transcription template of the crRNA is shown in SEQ ID NO: 7 and SEQ ID NO: 9.Based on the composition, the present application further provides a method for detecting Burkholderia mallei.The method combines RPA amplification with a CRISPR-Cas12a system, and has a sensitivity of a detection lower limit of 2.7 copies / μL and stronger specificity, and can detect whether the sample contains Burkholderia mallei within 1h without the aid of large instruments and equipment, which is helpful for rapid diagnosis and screening of glanders.

This invention relates to the field of molecular diagnostics, specifically providing a diagnostic kit for the combined detection of DLBCL based on plasma exosomal miRNAs. This invention provides two or more peripheral blood exosomes used as diagnostic biomarkers for diffuse large B-cell lymphoma (DLBCL). Based on these DLBCL diagnostic biomarkers, this invention also provides a non-invasive, highly reproducible, and highly specific plasma exosomal miRNA diagnostic kit for the early diagnosis and follow-up monitoring of DLBCL. The plasma exosomal miRNA diagnostic kit provided by this invention is of great significance in the early diagnosis, genotyping, relapse monitoring, and efficacy evaluation of DLBCL.

The invention belongs to the technical field of biomedicine and molecular diagnosis, and particularly relates to a combined marker and a risk scoring model for prognosis prediction of lung adenocarcinoma based on lactylation related genes as well as a construction method and application of the combined marker and the risk scoring model, and the combined marker comprises the following 12 genes: ANGPTL4, ITGA6, SOD1, CCL20, FKBP3, DECR1, SEMA3C, TRIM28, VEGFC, TNNC2, CRTAC1 and HGF. On the basis of sequencing data of large-scale lung adenocarcinoma samples, a group of lactylation related genes closely related to the total lifetime are systematically screened out, and a risk scoring model is constructed. According to the model, survival risk layering can be accurately carried out on the lung adenocarcinoma patient, and reliable reference is provided for clinical treatment strategy formulation and individualized management. In addition, the lactylation related genes are newly found, and the lactylation process in the lung adenocarcinoma can be understood.

The invention discloses an i-motif DNA (deoxyribonucleic acid) modified gold nano flare, a preparation method of the i-motif DNA modified gold nano flare and application of the i-motif DNA modified gold nano flare to detection of miRNA, and belongs to the technical field of nano biological materials and molecular diagnosis. The fluorescence probe is formed by assembling gold nanoparticles and an i-motif molecular fluorescence probe, the i-motif molecular fluorescent probe contains four sections of sequences rich in C basic groups and a target miRNA recognition chain, sulfydryl is marked at the 5'end, and fluorescent molecules are marked at the 3 'end. The probe is prepared by adopting a freeze thawing method, the labeling density of the probe is high, in a constructed detection system, the miR-21 detection limit is as low as 1.01 fM, and single-base mutants can be accurately distinguished; the technology does not need complex sample pretreatment and can be directly used for detecting cell total RNA or miRNA in a plasma sample, and the detection result is highly consistent with RT-qPCR; the problems of low marking efficiency and insufficient sensitivity in the prior art are effectively solved, and an innovative tool with high sensitivity, high specificity and clinical suitability is provided for tumor-associated miRNA molecular diagnosis.

The invention belongs to the technical field of molecular diagnosis, and particularly relates to a visual detection reagent for cyprinid type II herpesvirus based on an RPA-CRISPR-cas12a system and application of the visual detection reagent. By designing primers and screening corresponding crRNA, efficient amplification of RPA and high-specificity recognition of a CRISPR-Cas12a system are combined to realize detection of extremely low-content cyprinid type II herpesvirus nucleic acid, the detection limit can be as low as 1.6 copies / microliter, and compared with a traditional detection method, the detection method disclosed by the invention does not need expensive instruments and equipment and complex reagents, and has the advantages of simplicity, convenience, rapidness and convenience in operation, and can be widely applied to detection of cyprinid type II herpesvirus nucleic acid. The detection cost is reduced, and large-scale popularization and application are facilitated.

The invention relates to the technical field of molecular diagnosis, in particular to a composition for visually detecting a dengue virus and a chikungunya fever virus based on an RT-ERA-LbCas12a system and application of the composition. The invention provides a composition for detecting the dengue virus and the chikungunya virus, the composition comprises an RT-ERA primer, crRNA, LbCas12a protease and an ssDNA fluorescent probe based on the composition, and the invention further provides a method for detecting the dengue virus and the chikungunya virus. According to the method, through the combination of RT-ERA amplification and a CRISPR-Cas12a system, the sensitivity with the lower detection limits of 1 copy / [mu] L, 2.4 copy / [mu] L and 1.5 copy / [mu] L and higher specificity are obtained respectively, whether a sample contains the dengue virus and the chikungunya fever virus or not can be detected within 1 h, and rapid diagnosis and screening of the dengue virus and the chikungunya fever virus are facilitated.

The invention discloses a marker LGALS9 for diagnosing nasopharyngeal squamous carcinoma and application thereof, and belongs to the technical field of molecular diagnosis. The invention finds that the LGALS9 molecule has significant high expression in nasopharyngeal squamous cell carcinoma tissues, prompts that the LGALS9 can be used as a unique marker for diagnosing the nasopharyngeal squamous cell carcinoma, further constructs a diagnosis model by taking the LGALS9 as the marker, preferably detects the expression level of the marker LGALS9 in the cancer tissues by adopting an immunohistochemical method, and unexpectedly finds that the LGALS9 molecule can be used for diagnosing the nasopharyngeal squamous cell carcinoma tissues. The diagnostic performance of the diagnostic model constructed according to an immunohistochemical detection result is superior to that of a model constructed by adopting a protein spectrum detection method. The marker LGALS9 and the diagnostic model provided by the invention provide technical support for tissue traceability of nasopharyngeal squamous carcinoma, and have great clinical transformation potential.

This invention belongs to the field of molecular diagnostics, specifically relating to biomarkers for the diagnosis of herpes simplex keratitis (HSK) and their applications. This invention is the first to demonstrate that C1QA and FCERT1G are specific and reliable inflammation-related biomarkers for HSK. Diagnostic and assessment methods based on these biomarkers can provide crucial molecular evidence for the early detection, accurate diagnosis, objective severity grading, and personalized treatment of HSK. Simultaneously, this invention reveals the potential roles of FCERT1G and C1QA in HSK and provides new ideas for targeted therapy of this disease, particularly the application of goserelin as an inflammation modulator, offering a new direction for HSK treatment.

The application belongs to the technical field of food detection and molecular diagnosis, and particularly relates to a molecular diagnosis system for food source component identification. The system comprises a handheld shell, and an automatic sample processing unit, a molecular detection unit, a central control unit and a data transmission unit are integrated in the shell. The automatic sample processing unit comprises at least two processing modules and an automatic transfer mechanism, which are used for crushing, lysing and automatic transfer of a solid sample, and an in-situ cleaning and disinfecting assembly is integrated. The application realizes full-process automation, handholding and intelligence from a solid sample to a detection result, and provides an innovative solution for food safety on-site rapid detection and intelligent supervision.

The invention provides a primer probe combination, a kit and a method for synchronously detecting PCV2 and Mhp, and belongs to the technical field of animal epidemic disease molecular diagnosis. According to the technical scheme provided by the invention, the porcine circovirus type 2 and the mycoplasma hyopneumoniae can be simultaneously, rapidly and accurately detected in one reaction system, the operation steps are remarkably simplified, the detection efficiency is improved, and the detection cost is reduced. The method is high in sensitivity, and infection can be found early; the specificity is strong, the cross reaction is effectively avoided, and the result is reliable. The matched kit is convenient for standardized production and clinical popularization, provides an efficient and convenient technical tool for mixed infection monitoring of the two important pathogens in a pig farm and early diagnosis and precise prevention and control of epidemic situations, and has good application prospects and market values.

The invention belongs to the technical field of microbiological detection and molecular diagnosis, and discloses a primer group, a kit and a detection method for detecting salmonella enteritidis, the kit comprises an immunocapture component, an isothermal amplification component, a CRISPR / Cas12a detection component and auxiliary components; the isothermal amplification component is any one of an RPA (recombinase polymerase amplification) component or an LAMP (loop-mediated isothermal amplification) component, the RPA component comprises a specific primer group targeting 86th to 212th nucleotides in a salmonella enteritidis hilA gene coding region, and the LAMP component comprises a specific primer group targeting 58th to 250th nucleotides in the salmonella enteritidis hilA gene coding region. The primer group, the kit and the detection method for detecting the salmonella enteritidis successfully integrate three key technologies of RAP and LAMP rapid isothermal amplification, CRISPR / Cas12a high-specificity recognition and trans-cleavage and immunomagnetic bead targeted enrichment, and creatively provide the primer group, the kit and the detection method for detecting the salmonella enteritidis.

This invention discloses molecular markers for lung cancer diagnosis, chemotherapy, or prognosis detection, and their applications, relating to the field of molecular diagnostics. The molecular markers include trypsin 3 splice variant 3 and the transcription factor bone marrow zinc finger gene 1 splice variant 2. By detecting the expression levels of the MZF1-V2 and PRSS3-V3 genes, lung cancer diagnosis, chemotherapy, or prognosis can be achieved. The introduction of these molecular markers has significant scientific and clinical value in personalized precision treatment applications that improve chemotherapy sensitivity. It not only provides effective molecular targets for personalized precision treatment of lung cancer patients but also provides new evidence for novel intervention strategies. Furthermore, the introduction of these molecular markers provides a more reliable means of lung cancer diagnosis and prognosis prediction.

The invention belongs to the technical field of molecular diagnosis, and particularly relates to a composition, a kit and a detection method for detecting burkholderia pseudomallei based on a double-target RPA-lateral chromatography test strip. The composition comprises a first RPA primer pair and a first probe aiming at a first target gene of burkholderia pseudomallei, and a second RPA primer pair and a second probe aiming at a second target gene, the 5'ends of the first probe and the second probe are respectively marked with different reporter groups which can be identified by the lateral chromatography test strip. The detection method comprises the following steps: extracting genome DNA of a sample to be detected; carrying out RPA constant-temperature amplification reaction by using the composition; and diluting the amplification product, dropwise adding the diluted amplification product to a sample adding area on the lateral chromatography test strip, and reading a result. Through double-target double confirmation, false negative leak detection caused by single-target gene mutation is effectively avoided, and the detection accuracy is improved; the detection method is simple and convenient to operate, low in cost, free of complex instruments, visible in result and especially suitable for rapid screening on a base site.

The invention belongs to the technical field of molecular diagnosis, and particularly relates to a miRNA amplification-free detection system and a preparation method and application thereof. The invention provides a miRNA detection system. The miRNA detection system comprises a CRISPR / Cas12a system, a double-labeled magnetic probe and a test strip. The detection system provided by the invention can improve the detection sensitivity and specificity of miRNA under the condition of no nucleic acid amplification; normal color development of a control line of the test strip can be ensured, and reliable interpretation of a result is realized; the device supports rapid, visual and portable result reading and data processing, and is suitable for on-site instant detection.

The invention relates to the technical field of medical image analysis and molecular diagnosis. The invention discloses a multi-mode prognosis evaluation method for local advanced cervical cancer. The method comprises the following steps: acquiring a magnetic resonance T2 weighted image and a tumor tissue sample of the same patient before treatment; based on the magnetic resonance T2 weighted image, a first risk score is output through a deep learning prognosis prediction model, and first risk layering is completed; screening prognosis related key protein combinations based on proteomics expression data of tumor tissue samples to complete second risk stratification or clustering typing; and fusing the first risk layering result and the second risk layering / clustering typing result to generate a comprehensive multi-mode prognostic risk assessment report. According to the method, the MRI image and the proteomics data are fused through the full-automatic deep learning model, non-invasive prognosis evaluation of cervical cancer is realized, high-risk / low-risk patients can be accurately distinguished without manual sketching, individualized treatment decision is supported, and the clinical transformation value is remarkably improved.

The invention discloses an RPA-CRISPR / Cas-based rapid detection method for echinococcus shikonii, and belongs to the technical field of molecular diagnosis. The method comprises the following steps: extracting DNA of a sample to be detected; the specific primers are used for RPA isothermal amplification, a reaction system comprises a buffer solution, upstream and downstream primers, RPA enzyme, magnesium acetate and template DNA, and reaction is carried out at 39 DEG C for 20 minutes; and mixing the amplification product with LbCas12a protein, sgRNA and a fluorescence report probe, carrying out CRISPR / Cas reaction at 37 DEG C for 30 minutes, and judging the result through a real-time fluorescence signal. According to the method, constant-temperature rapid amplification is realized by utilizing RPA, high-sensitivity and high-specificity detection of the echinococcus shikonii can be realized under the field condition by combining high-specificity recognition and cleavage activity of CRISPR / Cas12a, the sensitivity reaches a single-digit copy number, and the method is simple and convenient to operate, does not need a complex instrument and is suitable for primary medical treatment and field quarantine.

The application belongs to the technical field of molecular diagnosis, and particularly relates to a group of extracellular vesicle proteins as biomarkers for diagnosing abdominal aortic aneurysm and application. The application early diagnoses abdominal aortic aneurysm through determination of circulating extracellular vesicle protein markers, and provides an effective tool for diagnosis of abdominal aortic aneurysm. The extracellular vesicle protein marker is any one or two or more molecular marker combinations of interleukin-4 (IL-4), interleukin-6 (IL-6), monocyte chemotactic protein-1 (MCP-1), nerve growth factor (Neurturin) and tumor suppressor M (Oncostatin-M). The application uses proteins in extracellular vesicles as specific markers for early diagnosis of abdominal aortic aneurysm, which is a less applied field in current early diagnosis of AAA. The specific proteins in EVs may be directly related to the pathological process of AAA, and are different from conventional serum markers, and have higher specificity and sensitivity.

The application belongs to the technical field of molecular diagnosis, and particularly relates to a digital PCR kit and method for accurately detecting the copy number of a CAR gene of a CAR-T cell and a lentivirus vector. The application first realizes the absolute quantification of CAR, Rev and an internal reference gene RNase P in a single tube simultaneously, and has strong universality. Based on the principle of digital PCR, the absolute copy number is directly obtained without a standard curve, and the result is accurate and highly repeatable. The detection lower limit reaches 0.01%, and extremely low abundance CAR-T residues or microresidual diseases can be detected. Primers and probes are designed for the conservative regions of CD3zeta, Rev and RNase P, and no cross reaction is verified, so that false positives are effectively avoided. The sample only needs gDNA, and there is no requirement for cell activity, and the sample is compatible with freezing and transportation. The kit is premixed and optimized, the process is automated, and 4-5 hours are needed. The kit is suitable for the whole chain of CAR-T drug research and development, production process quality control, clinical patient monitoring and safety evaluation.

The invention discloses a rapid detection method for echinococcus granulosus based on RPA-CRISPR / Cas, and belongs to the technical field of molecular diagnosis. The method comprises the following steps: extracting DNA of a sample to be detected; the specific primers are used for RPA isothermal amplification, a reaction system contains a buffer solution, upstream and downstream primers, RPA enzyme, magnesium acetate and template DNA, and reaction is carried out at 39 DEG C for 20 minutes; and mixing the amplification product with LbCas12a protein, sgRNA and a fluorescence report probe, carrying out CRISPR / Cas reaction at 37 DEG C for 30 minutes, and judging the result through a real-time fluorescence signal. Constant-temperature rapid amplification is achieved through RPA, high-sensitivity and high-specificity detection of the echinococcus granulosus can be achieved under the site condition by combining high-specificity recognition and trans-cleavage activity of CRISPR / Cas12a, the sensitivity reaches the single-site copy number, operation is easy and convenient, complex temperature control equipment is not needed, and the method is suitable for primary medical treatment and site quarantine.

The invention discloses a marker LGALS7 for diagnosing nasopharyngeal squamous cell carcinoma and application of the marker LGALS7, and belongs to the technical field of molecular diagnosis. Proteomics screening finds that LGALS7 is remarkably low in expression in nasopharyngeal squamous cell carcinoma tissues, which prompts that LGALS7 can be used as a biomarker for diagnosing nasopharyngeal squamous cell carcinoma. According to the invention, the diagnosis result obtained by detecting the LGALS7 by adopting an immunohistochemical method is higher in accuracy, and a support vector machine model is constructed based on the diagnosis result. A model evaluation result shows that the marker LGALS7 shows relatively high diagnosis specificity and sensitivity in a plurality of independent queues, squamous cell carcinoma from nasopharynx and squamous cell carcinoma from other parts can be effectively distinguished, and the problem of insufficient diagnosis specificity in the prior art is solved.

The application provides a microfluidic chip for diagnosing avian influenza H3 / H5 / H6 / H9 molecular typing based on microfluidics and a use method, relates to the field of pathogenic microorganism testing technology, and improves the efficiency, accuracy and convenience of avian influenza molecular diagnosis, promotes the development of molecular diagnosis technology, and uses the microfluidic chip body to be supported and used through the chip support, opens the protection layer attached to the microfluidic chip body by means of the light-proof assembly on the side, and can provide a light-proof environment again during and after the detection process, and can maintain a longer detection state.