25 results about "Cryptococcus" patented technology

The present invention belongs to the field of biomedicine, and specifically relates to an antimicrobial peptide derived from lactoferrin and its application. The antimicrobial peptide derived from lactoferrin, whose amino acid sequence is shown in any one of SEQ ID NOs: 1 to 98, has a broad-spectrum antimicrobial effect, especially significant antibacterial effect against Pseudomonas putida, Rhodococcus erythropolis, Bacillus subtilis, Malassezia furfur, Cryptococcus neoformans, and Candida albicans, and has great application value.

The invention relates to the technical field of pathogen detection, in particular to a primer probe combination and a kit for detecting various invasive fungi and application of the primer probe combination and the kit. According to the primer and probe combination provided by the invention, a digital PCR (Polymerase Chain Reaction) platform technology is utilized, so that the rapid, efficient, accurate, sensitive and high-specificity simultaneous quantitative detection of Aspergillus fumigatus, Aspergillus, Mucoriums, Cryptococcus and Pneumocystis jirovecii is realized. The rapid detection of the common invasive fungi in sputum, alveolar lavage fluid, urine, blood and other samples is realized, the detection process does not need culture, the detection period is short, the manpower is greatly reduced, and the detection efficiency is improved.

This invention relates to a microbial agent with highly efficient plastic degradation properties and its application, wherein the microbial agent contains *Cryptococcus lauriensis* strain with accession number CCTCC NO: M 2026232. Papiliotrema laurentii SNP7-2P strain and / or Achromobacterium with accession number CCTCC NO: M 2026231 Achromobacter strains FJ1-2L. These two microbial strains exhibit varying degrees of degradation effects on long-chain polymers in plastics, effectively and environmentally addressing plastic pollution and making them suitable for preparing green and environmentally friendly degradable plastic products.

The invention relates to the technical field of lung image marking auxiliary film reading, in particular to an auxiliary film reading system suitable for a patient suffering from a cryptococcus lung disease. The method comprises the following steps: firstly, acquiring continuous CT slice images of a lung, and extracting a foreground connected domain of a lung parenchyma region as a connected domain to be analyzed; further obtaining the blood vessel possibility of the target connected domain according to the monotonicity of the width change of the target connected domain; further according to the monotonicity of the area change of the to-be-analyzed connected domain at the same position, obtaining the blood vessel penetrating possibility of the target connected domain; obtaining the quasi-circular degree of the target connected domain; and finally, marking the target connected domain according to the quasi-circular degree, the blood vessel fusion possibility and the blood vessel penetration possibility. According to the method, the possibility that the target connected domain belongs to the blood vessel is measured from the angles of width change and area change, the blood vessel fusion possibility and the blood vessel penetrating possibility are fused by means of the quasi-circular degree, the connected domain is accurately marked, and related personnel are assisted in reading the film.

The application provides a fattening late-stage pig feed and a preparation method thereof, and relates to the technical field of pork breeding. The fattening late-stage pig feed is characterized by adding 10-15% of a nutritional supplement to per kilogram of fattening feed, and the nutritional supplement is composed of pine branch fermentation material, lotus root fermentation material, alfalfa powder, roxburgh rose extract and caffeine. The pine branch fermentation material is obtained by subjecting pine branches to cellulase enzymolysis treatment and then to fermentation by means of bifidobacterium and spencerolyn cryptococcus. The lotus root fermentation material is obtained by subjecting cooked lotus roots to fermentation by means of lactobacillus plantarum. The application overcomes the shortcomings of the prior art, effectively guarantees the weight gain efficiency of porkers in the late fattening stage, further improves pork quality, and comprehensively improves the economic benefits of pork breeding.

The present invention relates to methods and means for inhibiting or preventing the growth of fungal cells with at least one flavone selected from the group consisting of 5-methoxyflavone, 2′-methoxyflavone, 7-methoxyflavone, 3′,4′,5,7-tetrametoxyflavone, 3′,4′,5′,5,6,7-hexymethoxyflavone, 7,8-benzoflavone and 5,6-benzoflavone for inhibiting or preventing the growth of a fungal cell, wherein said flavone is used in combination with at least one further antimycotic compound selected from the group of azoles, both in an amount to exhibit a synergistic effect compared to the separate use of said flavone and said antimycotic compound.

The invention relates to application of azole drugs in preparation of antifungal drugs, and belongs to the technical field of research and development of antifungal drugs. According to the application of the glycyrrhetinic acid combined with the azoles in preparation of the antifungal drugs, the glycyrrhetinic acid combined with the itraconazole, the voriconazole and the posaconazole shows a good synergistic effect or antagonistic effect on aspergillus, ectobottle dermatitis, candida and cryptococcus neoformans, and the application of the glycyrrhetinic acid combined with the itraconazole, the voriconazole and the posaconazole in preparation of the antifungal drugs is disclosed. And a research and development direction is provided for preparing medicines for resisting aspergillus, ectobottle dermatitis, candida and cryptococcus neoformans.

The invention belongs to the technical field of biological detection, and particularly relates to a nitrogen-containing heterocyclic compound, a preparation method thereof, a fluorescent probe and application. The nitrogen-containing heterocyclic compound provided by the invention has a structure as shown in the following general formula (I): # imgabs0 #, is simple in structure, has good chemical and optical stability, can be used for imaging fungi such as cryptococcus neoformans, has application potential for judging fungal infection conditions, and in addition, has good application prospects. The compound can be combined with intein of fungi through sulfydryl reaction, shows good specificity as a targeted fluorescent probe, and realizes rapid evaluation and screening of Prp8 intein inhibitor candidate compounds.

A method for assessing the overall toxicity of multiple pollutants in wastewater is disclosed. The bending angle of each part of the Cryptococcus hypocastanum nematode is regarded as the posture of the nematode, and the posture of the nematode is identified using a posture feature extraction model, and the characteristics of the water sample to be measured are determined. The signature variables are obtained and the overall toxicity of the composite pollutants in the wastewater is further evaluated based on the characterization variables of the water sample to be measured.

This invention provides two dual-target antimicrobial peptides, their preparation methods, and applications, belonging to the field of bioengineering. Their sequences are shown as NLR-2 and NIR-3, with the N-terminus linked to the carboxyl group of 3-(1-naphthyl)acrylic acid. The two antifungal peptides of this invention exhibit good bactericidal activity against Cryptococcus neoformans under physiological conditions (1 μM), and also demonstrate good antifungal activity in corn feed, effectively reducing the toxin production in corn feed caused by Fusarium graminearum. Therefore, the antifungal peptides of this invention possess excellent bactericidal function, can target fungal membrane structures, achieving highly efficient membrane-breaking and bactericidal effects, and can be used as novel antifungal agents.

This invention provides a method for inhibiting the growth of a pathogenic mold by contacting the pathogenic mold with a synergistically effective amount of an NK1 antagonisfand an antifungal agent. This invention also provides a method for inhibiting the growth of a mammalian fungal pathogen by contacting the mammalian fungal pathogen with a synergistically effective amount of posaconazole and rolapitant. In some aspects, the mammalian fungal pathogen is a species of Candida (e.g., C. glabrata, C. auris, C. albicans, C. dubliniensis, or C. parapsilosis). Aspergillus, Cryptococcus, Mucor, orRhizopus. In addition, A surface disinfectant comprising a synergistic amount of an NK1 antagonist and an antifungal agent in admixture with a carrier or excipient.

The invention relates to the technical field of biological detection, and discloses a composition for detecting respiratory tract infection pathogens, a kit and application. The composition disclosed by the invention can be used for rapidly and simultaneously detecting legionella pneumophila, bordetella pertussis, chlamydia pneumoniae, mycoplasma pneumoniae, mycobacterium tuberculosis, aspergillus and cryptococcus neoformans in a sample with high specificity, and has relatively high sensitivity and stability. Compared with traditional detection methods such as pathogen isolated culture and genome sequencing, the efficiency of identifying and jointly detecting multiple respiratory tract pathogens by adopting the composition disclosed by the invention is effectively improved, the detection process is simple and convenient to operate, and the composition is suitable for large-scale popularization and application.

This invention discloses a method for disrupting the cell walls of structurally heterogeneous microorganisms based on the synergistic vibration of multi-size media and its application. The method includes the following steps: (1) constructing a composite cell-dispersing media system with multi-size characteristics and filling it into a cell-dispersing tube; (2) injecting the microbial sample to be disrupted into the cell-dispersing tube and oscillating it at a linear velocity of 6-7 m / s for 30-90 seconds at 4-25 ℃; (3) after cell disruption, separating the mixed composite cell-dispersing media system and the solution to obtain a cell-dispersed solution containing intracellular products. The beneficial effects of this invention are: through the synergistic effect of multi-scale shear forces, it significantly improves the lysis efficiency of difficult-to-disrupt pathogens such as Mycobacterium tuberculosis, Cryptococcus, and Aspergillus, effectively protects the nucleic acid integrity of RNA viruses, has high cell disruption uniformity, good RNA protection, can be standardized and adapted to automated platforms, and is suitable for the pretreatment of complex clinical samples.

The present invention relates to antifungal oligopeptide compounds according to Formula I, Asp–R2–Gly–Leu–R5–R6–R7–Leu–Gly–R10–R11–R12 (Formula I), wherein R2 represents Arg, Lys, Cit, D-Arg or Orn; R5 represents Phe, Arg, Lys, His, Trp, Tyr, D-Tyr, D-Phe, Orn, 4-aminophenylalanine or 3-phenylpropionate; R6 represents Asp, Pro, Leu, Glu or Lys; R7 represents Phe or Trp; each of R10, R11, and R12 independently represents Lys, Arg or Orn; that are effective in therapeutic and research applications related to human pathogenic fungi, particularly yeasts Candida albicans and Cryptococcus neoformans. Additionally, the invention covers the use of these antifungal oligopeptides in the treatment of fungal infections, particularly those affecting the central nervous system (CNS) – by the administration of a therapeutically effective amount of any of these oligopeptide compounds.

Provided are: a novel therapeutic agent for fungal infections caused by Candida auris or Cryptococcus neoformans, which have acquired resistance to an azole-based antifungal agent; and a method for identifying resistant fungal strains, the method being performed prior to treatment with said therapeutic agent.　The therapeutic agent is characterized by combining an azole-based antifungal agent and milbemycin as active ingredients. The identifying method is characterized by using an azole-based antifungal agent-added and milbemycin-added fungal isolation medium and an azole-based antifungal agent-added and milbemycin-free fungal isolation medium.

The present invention relates to a method for screening an antifungal agent against Cryptococcus neoformans and an antifungal composition. Utilizing a newly identified gene essential for the growth and survival of Cryptococcus neoformans, the screening method of the present invention can effectively screen an antifungal agent.

The application relates to the application of azole drugs in the preparation of antifungal drugs and belongs to the technical field of antifungal drug research and development. The application of glycyrrhetic acid combined with azole drugs in the preparation of antifungal drugs discloses that glycyrrhetic acid combined with itraconazole, voriconazole and posaconazole shows good synergistic effect or antagonistic effect on aspergillus, exophiala dermatiditis, candida and new cryptococcus, and provides a research and development direction for preparing drugs for resisting aspergillus, exophiala dermatiditis, candida and new cryptococcus.

The present application relates to the technical field of wine brewing, in particular to a method for brewing Thompson Seedless white grape wine by carbon dioxide impregnation fermentation, comprising the following steps: adopting a process scheme combining carbon dioxide whole fruit impregnation and sequential inoculation of multiple yeasts, placing whole Thompson Seedless white grape berries in a closed carbon dioxide environment for impregnation at 28-32 DEG C for 8-12 days to promote the dissolution and conversion of skin aroma precursors; after crushing, juice extraction and clarification, first inoculating non-wine yeast such as Cryptococcus or Saccharomycodes deemii for fermentation for 48 hours, and then inoculating wine yeast QA23 or EC1118 to complete main fermentation and segmented temperature control. The present application significantly increases the content of ester and terpene aroma substances, enhances fruitiness and floral characteristics, effectively improves the complexity and level of aroma, guarantees fermentation stability, and provides a new technical approach for the quality upgrading of Thompson Seedless white grape wine.

The invention relates to the technical field of pathology, in particular to a combined strain cell positive control wax block and a preparation method thereof. The preparation method comprises the following steps: strain samples are placed in an inactivation stationary liquid for inactivation treatment to obtain an inactivated strain suspension, the strain samples comprise aspergillus, cryptococcus, talaromyces marneffei and mycobacterium tuberculosis, and the inactivation stationary liquid comprises ethanol and a formaldehyde solution; carrying out centrifugal treatment on the inactivated strain suspension to obtain a precipitate, dissolving the precipitate in an agar solution, and then carrying out solidification treatment to obtain an agar block wrapping the inactivated strain; and sequentially carrying out dehydration treatment and embedding treatment on the agar block to obtain the combined strain cell positive control wax block. According to the method, a large number of positive control wax blocks of various fungi and mycobacterium tuberculosis cells can be rapidly manufactured, the positive bacteria are more, the background is clear, interference is little, misjudgment is not easily caused, positive bacteria can be rapidly observed, and the method can be widely applied to positive control of various morphological experiments.

The invention belongs to the technical field of microbial fermentation. The invention provides a mutant strain delta wbpO, which is obtained by knocking out a wbpO gene in stenotrophomonas maltophilia CGMCC (China General Microbiological Culture Collection Center) NO. 1.1788. The base sequence of the wbpO gene is as shown in SEQ ID NO. 1 in a sequence table. The invention also provides an outer membrane vesicle of the mutant strain delta wbpO. The outer membrane vesicle of the mutant strain [delta] wbpO provided by the invention has an effect of inhibiting or killing cryptococcus neoformans (C. neoformans).

The invention discloses an application of natamycin acetate in preparation of a medicine for treating invasive fungal diseases. The invention provides application of natamycin acetate in treating diseases caused by invasive cryptococcus neoformans, such as cryptococcus neoformans meningitis, and a new treatment method is provided for the diseases caused by the invasive cryptococcus neoformans.

The application belongs to the field of biological medicine and particularly relates to a kind of detection of a variety of respiratory fungal convection amplification primer pair and probe and application. The convection amplification primer pair includes aspergillus fumigatus primer pair, cryptococcus neoformans primer pair and pneumocystis jirovecii primer pair, and the primer sequence in aspergillus fumigatus primer pair, cryptococcus neoformans primer pair and pneumocystis jirovecii primer pair includes target nucleic acid complementary sequence and interface sequence, and the interface sequence is not complementary with the target nucleic acid; folding sequence is provided in the target nucleic acid complementary sequence, so that the primer sequence has a curved hairpin structure. The primer probe is designed at the same time, and a respiratory fungal multiplex detection kit is prepared. The multiplex detection can avoid non-specific amplification and improve the detection sensitivity.

A special fertilizer for corn suitable for saline-alkali soil, a preparation method therefor, and a use thereof, belonging to the technical field of fertilizers. The special fertilizer comprises a modifier and a compound microbial agent. The modifier comprises veratramine, zeolite powder, biochar, and humic acid, and the dosage ratio of the veratramine, the zeolite powder, the biochar, and the humic acid is (3-6):(3-5):(1-2):(5-8). The compound microbial agent comprises Cryptococcus and Trichothecium roseum, the preservation number of the Cryptococcus is GDMCC 2.205, and the preservation number of the Trichothecium roseum is GIM 3.500. The introduction of the modifier and the compound microbial agent into a traditional base fertilizer enables said fertilizer to effectively improve the quality of corn, and also prevent and control the infection of corn by Bipolaris maydis.

The invention belongs to the technical field of biological prevention and control of fruits and vegetables, and particularly relates to application of cryptococcus sp. Y3 to control grape black mold. The method comprises the following steps: carrying out activated culture on cryptococcus lactis Y3, centrifuging to obtain thalli, preparing a yeast suspension by using sterile normal saline, punching the surface of a grape, injecting the yeast suspension, and then injecting an A.niger spore suspension, thereby effectively controlling the grape aspergillus niger disease. Or the yeast suspension is directly and uniformly sprayed on the surfaces of the grapes, and the grapes are naturally aired, so that the control of the aspergillus niger disease after harvesting of the grapes can be realized. According to the method, the infection of the aspergillus niger on the grapes can be efficiently controlled by using the cryptococcus sp. Y3 for the first time, and the control effect is remarkable; within 3 days after treatment, cryptococcus lactis Y3 successfully inhibits aspergillus niger from infecting grape pulp; after treatment for 24 hours, the disease incidence of the aspergillus niger is reduced by 28.56% through the cryptococcus lactis Y3, and the cryptococcus lactis is convenient to operate, safe, environmentally friendly and wide in market application prospect.

The invention belongs to the field of natural medicines and chemical medicines, and particularly relates to an imidazolyl-containing alkyne compound and application thereof in preparation of antifungal medicines. The imidazolyl-containing alkyne compound is a compound as shown in a formula (I) or a medicinal salt or a medicinal esterified derivative thereof. The alkyne compound containing the imidazolyl group is prepared by the following steps: crushing branches and leaves of Chieniodon hainanense (Merr.) Tsiamamp, performing percolation extraction by using ethanol, concentrating an extract, then extracting by using dichloromethane, and then performing silica gel column chromatography, octadecyl bonded silica gel column chromatography and preparative HPLC (High Performance Liquid Chromatography) separation and purification, thereby obtaining the alkyne compound containing the imidazolyl group. The imidazolyl-containing alkyne compound has remarkable antifungal activity on cryptococcus neoformans, candida albicans and fusarium solani at lower concentration, and can be used for preparing antifungal drugs or pesticides.