38 results about "Cryptococcus" patented technology

The invention particularly relates to a method for cultivating biological bait in an outdoor pond. According to the method, after an outdoor pond is disinfected and cleaned, a biological fertilizer source and various beneficial bacteria combination are used in a combined mode, cryptococcus ovale, composite diatom and chlorella are inoculated, a stable pond bacterium-algae system is constructed, and various cultivated microalgae are used for strengthening zooplanktons of rotifers, cladocerans and copepods in the bait pond. The breeding period of the biological bait is shortened, the survival rate of the biological bait is increased, the yield of the biological bait is increased, meanwhile, the nutritional value of the biological bait is enhanced, and opening breeding of the fry is facilitated. The breeding method is low in cost and suitable for various weathers, and sufficient bait guarantee is provided for large-scale breeding of fish fries.

The present invention belongs to the field of biomedicine, and specifically relates to an antimicrobial peptide derived from lactoferrin and its application. The antimicrobial peptide derived from lactoferrin, whose amino acid sequence is shown in any one of SEQ ID NOs: 1 to 98, has a broad-spectrum antimicrobial effect, especially significant antibacterial effect against Pseudomonas putida, Rhodococcus erythropolis, Bacillus subtilis, Malassezia furfur, Cryptococcus neoformans, and Candida albicans, and has great application value.

The present application is directed to a cochleate composition including one or more cochleates and at least two anti-fungal compounds selected from amphotericin B, 5-flucytosine, fluconazole, ketoconazole, ravuconazole, albaconazole, itraconazole, posaconazole, isavuconazole and / or voriconazole. Methods of using the antifungal cochleate formulations are also disclosed.

The invention relates to the technical field of pathogen detection, in particular to a primer probe combination and a kit for detecting various invasive fungi and application of the primer probe combination and the kit. According to the primer and probe combination provided by the invention, a digital PCR (Polymerase Chain Reaction) platform technology is utilized, so that the rapid, efficient, accurate, sensitive and high-specificity simultaneous quantitative detection of Aspergillus fumigatus, Aspergillus, Mucoriums, Cryptococcus and Pneumocystis jirovecii is realized. The rapid detection of the common invasive fungi in sputum, alveolar lavage fluid, urine, blood and other samples is realized, the detection process does not need culture, the detection period is short, the manpower is greatly reduced, and the detection efficiency is improved.

This invention relates to a microbial agent with highly efficient plastic degradation properties and its application, wherein the microbial agent contains *Cryptococcus lauriensis* strain with accession number CCTCC NO: M 2026232. Papiliotrema laurentii SNP7-2P strain and / or Achromobacterium with accession number CCTCC NO: M 2026231 Achromobacter strains FJ1-2L. These two microbial strains exhibit varying degrees of degradation effects on long-chain polymers in plastics, effectively and environmentally addressing plastic pollution and making them suitable for preparing green and environmentally friendly degradable plastic products.

The invention relates to the technical field of lung image marking auxiliary film reading, in particular to an auxiliary film reading system suitable for a patient suffering from a cryptococcus lung disease. The method comprises the following steps: firstly, acquiring continuous CT slice images of a lung, and extracting a foreground connected domain of a lung parenchyma region as a connected domain to be analyzed; further obtaining the blood vessel possibility of the target connected domain according to the monotonicity of the width change of the target connected domain; further according to the monotonicity of the area change of the to-be-analyzed connected domain at the same position, obtaining the blood vessel penetrating possibility of the target connected domain; obtaining the quasi-circular degree of the target connected domain; and finally, marking the target connected domain according to the quasi-circular degree, the blood vessel fusion possibility and the blood vessel penetration possibility. According to the method, the possibility that the target connected domain belongs to the blood vessel is measured from the angles of width change and area change, the blood vessel fusion possibility and the blood vessel penetrating possibility are fused by means of the quasi-circular degree, the connected domain is accurately marked, and related personnel are assisted in reading the film.

The application provides a fattening late-stage pig feed and a preparation method thereof, and relates to the technical field of pork breeding. The fattening late-stage pig feed is characterized by adding 10-15% of a nutritional supplement to per kilogram of fattening feed, and the nutritional supplement is composed of pine branch fermentation material, lotus root fermentation material, alfalfa powder, roxburgh rose extract and caffeine. The pine branch fermentation material is obtained by subjecting pine branches to cellulase enzymolysis treatment and then to fermentation by means of bifidobacterium and spencerolyn cryptococcus. The lotus root fermentation material is obtained by subjecting cooked lotus roots to fermentation by means of lactobacillus plantarum. The application overcomes the shortcomings of the prior art, effectively guarantees the weight gain efficiency of porkers in the late fattening stage, further improves pork quality, and comprehensively improves the economic benefits of pork breeding.

The present invention relates to methods and means for inhibiting or preventing the growth of fungal cells with at least one flavone selected from the group consisting of 5-methoxyflavone, 2′-methoxyflavone, 7-methoxyflavone, 3′,4′,5,7-tetrametoxyflavone, 3′,4′,5′,5,6,7-hexymethoxyflavone, 7,8-benzoflavone and 5,6-benzoflavone for inhibiting or preventing the growth of a fungal cell, wherein said flavone is used in combination with at least one further antimycotic compound selected from the group of azoles, both in an amount to exhibit a synergistic effect compared to the separate use of said flavone and said antimycotic compound.

The invention relates to application of azole drugs in preparation of antifungal drugs, and belongs to the technical field of research and development of antifungal drugs. According to the application of the glycyrrhetinic acid combined with the azoles in preparation of the antifungal drugs, the glycyrrhetinic acid combined with the itraconazole, the voriconazole and the posaconazole shows a good synergistic effect or antagonistic effect on aspergillus, ectobottle dermatitis, candida and cryptococcus neoformans, and the application of the glycyrrhetinic acid combined with the itraconazole, the voriconazole and the posaconazole in preparation of the antifungal drugs is disclosed. And a research and development direction is provided for preparing medicines for resisting aspergillus, ectobottle dermatitis, candida and cryptococcus neoformans.

The invention belongs to the technical field of biological detection, and particularly relates to a nitrogen-containing heterocyclic compound, a preparation method thereof, a fluorescent probe and application. The nitrogen-containing heterocyclic compound provided by the invention has a structure as shown in the following general formula (I): # imgabs0 #, is simple in structure, has good chemical and optical stability, can be used for imaging fungi such as cryptococcus neoformans, has application potential for judging fungal infection conditions, and in addition, has good application prospects. The compound can be combined with intein of fungi through sulfydryl reaction, shows good specificity as a targeted fluorescent probe, and realizes rapid evaluation and screening of Prp8 intein inhibitor candidate compounds.

The present invention relates to a derivative compound comprising an amino nicotinamide structure, an isomer thereof or a pharmaceutically acceptable salt thereof, and an antifungal pharmaceutical composition containing same. More specifically, the present invention provides benzyl-oxybenzylamine amino acid derivatives with reduced side effects, and antifungal agents containing same, which exhibit antifungal activity against a spectrum of fungi selected from the group consisting of Candida spp., Sporotrichum spp., Malassezia spp., Cryptococcus spp., Aspergillus spp., Pneumocystis spp., and Mucor spp.

The invention relates to the technical field of skin care products, and particularly discloses an acne removing composition for fading acne marks and a preparation method thereof. The acne-removing composition for fading acne marks is prepared by compounding an olive leaf extract, a clausena lansium bark extract, an artemisia annua extract, a podocarpus macrophyllus extract, a hydrolyzed opuntia ficus-indica flower extract and a silybum marianum extract. The preparation method of the acne-removing composition for fading acne marks comprises the following steps: 1) uniformly mixing an olive leaf extract, a clausena lansium bark extract, an artemisia annua extract, a podocarpus macrophyllus extract, a hydrolyzed opuntia ficus-indica flower extract, a silybum marianum extract and a solvent to obtain a plant extract; the invention relates to a preparation method of an acne-removing composition capable of fading acne marks, which comprises the following steps: 1) preparing a plant extract, 2) adding compound bacteria into the plant extract for fermentation to obtain a fermentation broth, and 3) sterilizing the fermentation broth, concentrating and drying to obtain the acne-removing composition capable of fading acne marks, the compound bacteria being a compound of cryptococcus laurentii, Wickerhamomyces anomalus and Candida boidinii, and the acne-removing composition capable of fading acne marks has the advantages of rapidly removing acnes and rapidly fading acne marks.

The present invention relates to the technical field of skin care products, and specifically discloses a composition containing superoxide dismutase (SOD) and a preparation method thereof. The composition containing superoxide dismutase (SOD) comprises the following components: 28-32 parts of yeast fermentation product filtrate; 0.8-1.2 parts of garlic bulb extract; 0.8-1.2 parts of superoxide dismutase (SOD); 0.4-0.6 parts of angelica extract; 65-70 parts of water; the preparation method of the yeast fermentation product filtrate is as follows: 1), mix broom, hops, hibiscus bark, and perilla leaves evenly to obtain a plant mixture; 2), mix the plant mixture with water evenly to obtain a plant mixture; 3), inoculate bumblebee Candida albicans and Cryptococcus laurentii into the plant mixture, ferment for 7-9 days, filter, and sterilize to obtain a yeast fermentation product filtrate. The present invention has the advantages of improving the anti-wrinkle and firming effect to achieve rapid wrinkle reduction.

The invention provides two double-target antibacterial peptides as well as a preparation method and application thereof, and belongs to the field of bioengineering, the sequences of the double-target antibacterial peptides are shown as NLR-2 and NIR-3, and the N end of the double-target antibacterial peptides is connected with carboxyl of 3-(1-naphthyl) acrylic acid. The two antifungal peptides disclosed by the invention have good bactericidal activity (1 mu M) on cryptococcus neoformans under physiological conditions, and meanwhile, have a relatively good mildew-proof effect on corn feed, and can effectively reduce the toxin yield of the corn feed caused by fusarium graminearum. Therefore, the antifungal peptide provided by the invention has a good bactericidal function, can target a fungal membrane structure, achieves the effects of efficient membrane rupture and sterilization, and can be used as a novel mildew preventive.

A method for assessing the overall toxicity of multiple pollutants in wastewater is disclosed. The bending angle of each part of the Cryptococcus hypocastanum nematode is regarded as the posture of the nematode, and the posture of the nematode is identified using a posture feature extraction model, and the characteristics of the water sample to be measured are determined. The signature variables are obtained and the overall toxicity of the composite pollutants in the wastewater is further evaluated based on the characterization variables of the water sample to be measured.

This invention provides two dual-target antimicrobial peptides, their preparation methods, and applications, belonging to the field of bioengineering. Their sequences are shown as NLR-2 and NIR-3, with the N-terminus linked to the carboxyl group of 3-(1-naphthyl)acrylic acid. The two antifungal peptides of this invention exhibit good bactericidal activity against Cryptococcus neoformans under physiological conditions (1 μM), and also demonstrate good antifungal activity in corn feed, effectively reducing the toxin production in corn feed caused by Fusarium graminearum. Therefore, the antifungal peptides of this invention possess excellent bactericidal function, can target fungal membrane structures, achieving highly efficient membrane-breaking and bactericidal effects, and can be used as novel antifungal agents.

This invention provides a method for inhibiting the growth of a pathogenic mold by contacting the pathogenic mold with a synergistically effective amount of an NK1 antagonisfand an antifungal agent. This invention also provides a method for inhibiting the growth of a mammalian fungal pathogen by contacting the mammalian fungal pathogen with a synergistically effective amount of posaconazole and rolapitant. In some aspects, the mammalian fungal pathogen is a species of Candida (e.g., C. glabrata, C. auris, C. albicans, C. dubliniensis, or C. parapsilosis). Aspergillus, Cryptococcus, Mucor, orRhizopus. In addition, A surface disinfectant comprising a synergistic amount of an NK1 antagonist and an antifungal agent in admixture with a carrier or excipient.

The invention relates to the technical field of fungal etiology detection, and discloses a method for accurately detecting cryptococcus neoformans, which comprises the following steps: S1, selecting a sample to be detected; s2, direct smear microscopic examination; s3, culturing; and S4, detecting. One of cerebrospinal fluid, blood and sputum of a patient is adopted as a sample to be detected, and timely detection or proper preservation is needed after collection. In the step S2, the sample is mixed with a proper amount of Indian ink, the sample is observed under a microscope, the capsule of the cryptococcus neoformans is found, and the cryptococcus neoformans can present obvious capsule and a transparent halo in a black background and can be easily recognized. And S3, inoculating the sample on a proper culture medium, and observing whether cryptococcus neoformans grow or not. The method adopted by the invention is rapid, simple and convenient, not only has higher sensitivity and specificity, but also can improve the accuracy and reliability of cryptococcus neoformans detection.

The invention relates to the technical field of biological detection, and discloses a composition for detecting respiratory tract infection pathogens, a kit and application. The composition disclosed by the invention can be used for rapidly and simultaneously detecting legionella pneumophila, bordetella pertussis, chlamydia pneumoniae, mycoplasma pneumoniae, mycobacterium tuberculosis, aspergillus and cryptococcus neoformans in a sample with high specificity, and has relatively high sensitivity and stability. Compared with traditional detection methods such as pathogen isolated culture and genome sequencing, the efficiency of identifying and jointly detecting multiple respiratory tract pathogens by adopting the composition disclosed by the invention is effectively improved, the detection process is simple and convenient to operate, and the composition is suitable for large-scale popularization and application.

The present invention discloses a sesterterpenoid compound, a preparation method thereof and an application thereof. In the present invention, two novel-structured sesterterpenoid compounds are obtained by co-culturing and fermenting two edible and medicinal fungi, Pleurotus ostreatus and Trametes robiniophila. The structural formulas are shown as Formula I or Formula II. The two sesterterpenoid compounds have significant activities of inhibiting Candida albicans and Cryptococcus neoformans, and can be applied to the research and development of antibacterial drugs; moreover, the preparation method described in the present invention opens up a new way for preparing new antibacterial preparations in the antibacterial field.

The invention provides an antibacterial peptide QS18 as well as a preparation method and application thereof, and belongs to the technical field of antibacterial peptides. Aiming at the public health problem of cryptococcus neoformans, the invention provides an antibacterial peptide QS18, and the amino acid sequence of the antibacterial peptide QS18 is shown as SEQ ID NO: 1. The antibacterial peptide QS18 has good inhibiting and killing effects on cryptococcus neoformans, is high in stability, has the advantages of low hemolytic activity and low cytotoxicity, is expected to become a novel candidate drug for resisting cryptococcus neoformans, and has a wide market application prospect.

The present invention relates to a screening method for blood-brain barrier crossing inhibitors against Cryptococcus neoformans, a screening method for antifungal agents, and an antifungal composition. Since the screening method of the present invention utilizes a novel gene that regulates the blood-brain barrier crossing of Cryptococcus neoformans, it is possible to effectively screen for blood-brain barrier crossing inhibitors and antifungal agents.

This invention discloses a method for disrupting the cell walls of structurally heterogeneous microorganisms based on the synergistic vibration of multi-size media and its application. The method includes the following steps: (1) constructing a composite cell-dispersing media system with multi-size characteristics and filling it into a cell-dispersing tube; (2) injecting the microbial sample to be disrupted into the cell-dispersing tube and oscillating it at a linear velocity of 6-7 m / s for 30-90 seconds at 4-25 ℃; (3) after cell disruption, separating the mixed composite cell-dispersing media system and the solution to obtain a cell-dispersed solution containing intracellular products. The beneficial effects of this invention are: through the synergistic effect of multi-scale shear forces, it significantly improves the lysis efficiency of difficult-to-disrupt pathogens such as Mycobacterium tuberculosis, Cryptococcus, and Aspergillus, effectively protects the nucleic acid integrity of RNA viruses, has high cell disruption uniformity, good RNA protection, can be standardized and adapted to automated platforms, and is suitable for the pretreatment of complex clinical samples.

The present invention relates to antifungal oligopeptide compounds according to Formula I, Asp–R2–Gly–Leu–R5–R6–R7–Leu–Gly–R10–R11–R12 (Formula I), wherein R2 represents Arg, Lys, Cit, D-Arg or Orn; R5 represents Phe, Arg, Lys, His, Trp, Tyr, D-Tyr, D-Phe, Orn, 4-aminophenylalanine or 3-phenylpropionate; R6 represents Asp, Pro, Leu, Glu or Lys; R7 represents Phe or Trp; each of R10, R11, and R12 independently represents Lys, Arg or Orn; that are effective in therapeutic and research applications related to human pathogenic fungi, particularly yeasts Candida albicans and Cryptococcus neoformans. Additionally, the invention covers the use of these antifungal oligopeptides in the treatment of fungal infections, particularly those affecting the central nervous system (CNS) – by the administration of a therapeutically effective amount of any of these oligopeptide compounds.

Provided are: a novel therapeutic agent for fungal infections caused by Candida auris or Cryptococcus neoformans, which have acquired resistance to an azole-based antifungal agent; and a method for identifying resistant fungal strains, the method being performed prior to treatment with said therapeutic agent.　The therapeutic agent is characterized by combining an azole-based antifungal agent and milbemycin as active ingredients. The identifying method is characterized by using an azole-based antifungal agent-added and milbemycin-added fungal isolation medium and an azole-based antifungal agent-added and milbemycin-free fungal isolation medium.

The present invention relates to a method for screening an antifungal agent against Cryptococcus neoformans and an antifungal composition. Utilizing a newly identified gene essential for the growth and survival of Cryptococcus neoformans, the screening method of the present invention can effectively screen an antifungal agent.

The application relates to the application of azole drugs in the preparation of antifungal drugs and belongs to the technical field of antifungal drug research and development. The application of glycyrrhetic acid combined with azole drugs in the preparation of antifungal drugs discloses that glycyrrhetic acid combined with itraconazole, voriconazole and posaconazole shows good synergistic effect or antagonistic effect on aspergillus, exophiala dermatiditis, candida and new cryptococcus, and provides a research and development direction for preparing drugs for resisting aspergillus, exophiala dermatiditis, candida and new cryptococcus.

The invention discloses a special wheat fertilizer suitable for saline-alkali soil as well as a preparation method and application thereof, and belongs to the technical field of fertilizers. Wherein the modifier is prepared from lauric acid, zeolite powder, propylparaben and polyaspartic acid salt, and the dosage ratio of the lauric acid to the zeolite powder to the propylparaben to the polyaspartic acid salt is (3 to 6) to (2 to 6) to (6 to 10) to (8 to 14); wherein the complex microbial inoculants are purchased from Guangdong Province Microbial Culture Collection Center and comprise cryptococcus and micrococcus luteus, the preservation number of the cryptococcus is GDMCC 2.205, and the preservation number of the micrococcus luteus is GDMCC NO: 61867. According to the invention, the modifier and the complex microbial inoculant are introduced into the traditional basic fertilizer, so that the fertilizer can effectively improve the quality of wheat and prevent and treat the infection of gaeumannomyces graminis.

The invention provides a multi-gene identification primer group for cryptococcus neoformans related to lower respiratory tract infection and application of the multi-gene identification primer group, and belongs to a gene detection technology. According to the invention, the FAM fluorescence is used for labeling the primers, a reaction system is optimized, target genes of cryptococcus neoformans, human reference genes and system internal reference can be detected in the same PCR system, deviation caused by uneven amplification in traditional PCR is overcome, and gene expression is accurately quantified. The reagent and the kit constructed based on the method are simple to operate, low in cost and high in accuracy, the capillary electrophoresis analysis technology is combined, the result is visual and reliable, large-scale clinical application is facilitated, and an efficient scheme is provided for LRTI related cryptococcus neoformans detection.

The present application relates to the technical field of wine brewing, in particular to a method for brewing Thompson Seedless white grape wine by carbon dioxide impregnation fermentation, comprising the following steps: adopting a process scheme combining carbon dioxide whole fruit impregnation and sequential inoculation of multiple yeasts, placing whole Thompson Seedless white grape berries in a closed carbon dioxide environment for impregnation at 28-32 DEG C for 8-12 days to promote the dissolution and conversion of skin aroma precursors; after crushing, juice extraction and clarification, first inoculating non-wine yeast such as Cryptococcus or Saccharomycodes deemii for fermentation for 48 hours, and then inoculating wine yeast QA23 or EC1118 to complete main fermentation and segmented temperature control. The present application significantly increases the content of ester and terpene aroma substances, enhances fruitiness and floral characteristics, effectively improves the complexity and level of aroma, guarantees fermentation stability, and provides a new technical approach for the quality upgrading of Thompson Seedless white grape wine.