127 results about "Cryptococcus" patented technology

The present invention provides C. neoformans genes that are essential and are potential targets for drug screening. The nucleotide sequence of the target genes can be used for various drug discovery purposes, such as expression of the recombinant protein, hybridization assay and construction of nucleic acid arrays. The uses of proteins encoded by the essential genes, and genetically engineered cells comprising modified alleles of essential genes in various screening methods are also encompassed by the invention. The present invention also provides methods and compositions that enable the experimental determination as to whether any gene in the genome of Cryptococcus neoformans is essential, and whether that gene is required for virulence or pathogenicity. The identification of essential genes and those genes critical to the development of virulent infections, provides a basis for the development of screens for new drugs against C. neoformans.

The invention discloses a fluorescence quantitative PCR primer, a probe and a kit for detecting ordinary pathogenic fungi. A specific primer and a TaqMan probe are independently designed, and a fluorescence PCR detection method which can be used for simultaneously detecting 15 clinically ordinary pathogenic fungi, including 8 candida mycoderma bacteria (candida albicans, candida glabrata, candida parapsilosis, candida kefyr, candida sake, candida kruse, candida guilliermind and candida tropicalis), four aspergilli (aspergillus niger, aspergillus flavus, aspergillus terreus and aspergillus fumigates), cryptococcus, rhizopus oryzae and mucor circinelloides, is established. The method is relatively high in sensitivity and specificity, and has significant meanings for early diagnosis and treatment on invasive infections with fungi.

The antifungal and cancer cell growth inhibitory activities of 1-(3′,4′,5′-trimethoxyphenyl)-2-nitro-ethylene (TMPN) were examined. TMPN was fungicidal for the majority of 132 reference strains and clinical isolates tested, including those resistant to fluconazole, ketoconazole, amphotericin B or flucytosine. Minimum fungicidal concentration / minimum inhibitory concentration (MFC / MIC) ratios were ≦2 for 96% of Cryptococcus neoformans clinical isolates and 71% of Candida albicans clinical isolates. TMPN was fungicidal for a variety of other basidiomycetes, endomycetes and hyphomycetes, and its activity was unaffected by alterations in media pH. TMPN was slightly cytotoxic for murine and human cancer cell lines (GI50=1-4 μg / ml), and weakly inhibited mammalian tubulin polymerization (IC50=0.60 μg / ml). TMPN may also be used as a biochemical probe for tubulin and fungal dimorphism studies.

The invention discloses Cryptococcus capsules and a method for preparing the same. The method for preparing the Cryptococcus capsules comprises the step of mixing a solution of alginate, Cryptococcus, multivalence metal cations to obtain the Cryptococcus capsules. Experiments show that the viable bacterium rates of the Cryptococcus capsules prepared by the method are all above 70 percent; the highest viable bacterium rate is up to 90 percent; and the activity of bacteria is not reduced. The Cryptococcus capsules of the invention can be stored at both low and normal temperature, the effect of storage for one month at normal temperature is substantially the same as that of the storage at low temperature and the biological activity of the bacteria are not influenced. Therefore, the method has wide application prospect in the preparation of biological agents and the preservation of other organisms.

The invention discloses a method for processing disease control after the strawberry is picked by combining ozone and Cryptococcus laurentii, belonging to the biological control field after the fruits are picked. The method comprises the following steps: activating Cryptococcus laurentii, inoculating the Cryptococcus laurentii into NYDB culture medium for culturing 24h, centrifuging the Cryptococcus laurentii to obtain thalli, using sterile water to preparing the thalli into 1*108 / ml of thalli suspension; putting the strawberry into a plastic basket and arranging the strawberry into a self plastic curtain, starting an ozone generator for introducing air for 30s with the ozone concentration being 0.574mg / ml, maintaining for 10min, soaking the strawberry being processed by ozone in Cryptococcus lanrentii suspension for 30s, and naturally air drying the strawberry; and putting the strawberry into the plastic basket, sealing the plastic basket by a preservative film, and storing the strawberry under the room temperature. In the method, the ozone is utilized for improving the biological control effect of the Cryptococcus laurentii on the strawberry after being picked, the use is simple, the operation is convenient, the effect is good and the cost is low.

The invention discloses a calmodulin gene. The calmodulin gene is cloned in a cryptococcus humicolus BSLL1-1 bacterial strain, wherein the nucleotide sequence of the calmodulin gene is as shown in SEQ ID NO:1; a protein with an amino acid sequence shown in SEQ ID NO:2 is encoded by the gene; the protein is expressed in escherichia coli and the gene is further successfully expressed in saccharomyces cerevisiae. The aluminum resistance of the saccharomyces cerevisiae is increased due to the gene; genetically engineered bacteria can adsorb (or absorb) activated aluminum in a culture medium; and the genetically engineered bacterial strain of the saccharomyces cerevisiae has the application potential of reducing the activated aluminum content in acid soil.

The invention relates to a triple nucleic acid test kit. The kit comprises a thallus lysate, a positive quantitative reaction liquid, a negative control reaction liquid and a thallus test reaction liquid, wherein the thallus test reaction liquid contains specific primers, specific probes and doping fluorescent dye for aspergillus, Cryptococcus neoformans and candida albicans, the three specific oprimers are designed according to areas of ITS sequences of aspergillus, Cryptococcus neoformans and candida albicans, FAM, VIC and CY-5 are selected for mark report groups of the three specific probes, MGB serves as a quenching group, the excitation wavelength of the FAM group is 492 nm, and the emission wavelength is 517 nm; the excitation wavelength of the VIC group is 557 nm and the emission wavelength is 574 nm; the excitation wavelength of the CY-5 group is 646 nm and the emission wavelength is 664 nm. The triple nucleic acid test kit for aspergillus, Cryptococcus neoformans and candida albicans can assist in diagnosing infection caused by aspergillus, Cryptococcus neoformans and candida albicans.

The invention relates to the fungal polysaccharide preparation field, and concretely relates to a preparation method of Cryptococcus Neoformans capsular polysaccharide. The invention provides a method for preparing the Cryptococcus Neoformans capsular polysaccharide through utilizing cetyl trimethyl ammonium bromide. The method comprises the following steps: 1, processing a secretion supernatant containing the capsular polysaccharide through using calcium acetate and glacial acetic acid, and precipitating through using ethanol to prepare a crude capsular polysaccharide; and 2, processing a solution of the crude capsular polysaccharide through using the cetyl trimethyl ammonium bromide to obtain the purified capsular polysaccharide. The method has the advantages of simplicity, high efficiency, low cost, and effective avoiding of the use of toxic and harmful chemical reagents.

Disclosed are a preparation method and application of Anaphalis lactea essential oil. Volatile oil in Anaphalis lacteal is extracted by steam distillation and CO2 supercritical extraction, ingredients of the volatile oil are analyzed initially by gas chromatography / mass spectrography, 57 ingredients in the volatile oil are identified and account for more than 94% of the total volatile oil. Antibacterial and bactericidal activities of the volatile oil are researched by the Oxford cup method, and according to the research, the volatile oil has an evident killing or inhibiting function for bacteria, such as staphylococcus, streptococcus, enterobacter, salmonella, shigella, pseudomonas, bacillus, escherichia coli and the like, and fungi, such as candida, cryptococcus, penicillin, aspergillus, mucor, microsporum, trichophyton, epidermophyton and the like. Testing by DPPH (diphenylpicrylhydrazyl) method shows that the volatile oil has fine antioxidant activity. Owing to the fine activity, the volatile oil is widely applicable to the industries of foods, medicines, cosmetics, health-care products and the like.

Provided herein are substituted aromatic compounds, which are tRNA synthetase inhibitors, and hence can be used as antimicrobial agents. Compounds described herein can be used for the treatment or prevention of a condition caused by or contributed to by gram positive, gram negative, anaerobic bacteria or fungal organisms, more particularly against bacterium, for example, Staphylococci, Enterococci, Streptococci, Haemophilus, Moraxalla, Escherichia, Chlamydia, Mycoplasm, Legionella, Mycobacterium, Helicobacter, Clostridium, Bacteroides, Corynebacterium, Bacillus or Enterobactericeae, and fungal organisms, for example, Aspergillus, Blastomyces, Candida, Coccidiodes, Cryptococcus, Epidermophyton, Hendersonula, Histoplasma, Microsporum, Paecilomyces, Paracoccidiodes, Pneumocystis, Trichophyton, or Trichosporium. Processes for the preparation of these compounds, pharmaceutical compositions thereof, and methods of treating microbial infections are also provided.

The invention discloses a calcineurin catalytic subunit gene which is cloned in a cryptococcus humicolus BSLL1-1 strain. The nucleotide sequence of the calcineurin catalytic subunit gene is shown in SEQ ID NO:1 and is used for coding the protein with an amino acid sequence shown in SEQ ID NO:2; the protein is expressed in escherichia coli, and the calcineurin catalytic subunit gene is further successfully expressed in saccharomyces cerevisiae. According to the calcineurin catalytic subunit gene, the saccharomyces cerevisiae is enhanced in aluminum resistant capacity, genetically engineered bacteria are capable of adsorbing (or absorbing) activated aluminum contained in a culture medium, and the genetically engineered bacteria of the saccharomyces cerevisiae have application prospect of reducing the content of activated aluminum contained in acid soil.

The invention relates herba dracocephalum heterophylli volatile oil possessing the bacteriostatic and germicidal action and its application. The method adopts steam distillation to extract the herba dracocephalum heterophylli volatile oil and adopts gas-chromatography / mass spectra to analysis the volatile oil, identifying the 83 components and 89.83% total content. The volatile oil can kill and suppress the staphylococcus, streptococcus, gonococcus, enterobacter, Escherichia, salmonella, shigella, pseudomonas, bacillus, oidiomycin, cryptococcus, aspergillus, penicillium, mucor, epidermophytin, micromonospora, trichophyton and other bacteriums. The herba dracocephalum heterophylli volatile oil is used for foodstuffs, drugs, health products, cosmetic products and other industries.

The invention discloses an immunocolloidal gold test strip capable of detecting aspergillus and cryptococcus simultaneously. The immunocolloidal gold test strip comprises a test strip cassette and a matched test strip body, wherein the test strip cassette is provided with a sample cell and an observation window. The test strip body is formed by a rigid polyvinyl chloride lining plate, a nitrocellulose membrane, a left colloidal gold conjugate pad, a middle colloidal gold conjugate pad and a water absorption pad in a sequential lap joint mode, wherein the left colloidal gold conjugate pad is coated with colloidal-gold-labeled galactomannan resisting monoclonal antibodies and colloidal-gold-labeled bovine serum albumin-dinitrobenzene, the middle colloidal gold conjugate pad is coated with colloidal-gold-labeled cryptococcus capsular polysaccharide resisting monoclonal antibodies and colloidal-gold-labeled bovine serum albumin-dinitrobenzene, the nitrocellulose membrane is provided with a left detection line coated with galactomannan resisting monoclonal antibodies, a middle detection line coated with cryptococcus capsular polysaccharide resisting monoclonal antibodies, and a quality control line coated with dinitrobenzene resisting antibodies in sequence in parallel. The test strip is capable of detecting aspergillus and cryptococcus simultaneously, easy and convenient to operate, rapid and accurate in detection and high in sensitivity and specificity.

The invention relates to staining fluid and a method for rapidly detecting novel Cryptococcus. The staining fluid comprises 0.5-3.0g of AZO-blue, 1.0-10.0g of glacial acetic acid and 90-110g of distilled water. The method for rapidly detecting novel cryptococcus comprises the steps of preparation of the staining fluid, mixing, detection, counting and the like. The invention has the advantages of simple, convenient, rapid, sensitive and accurate detection, high specificity, easy preservation of the staining fluid, short staining time and excellent staining effect.

The invention relates to a colloidal gold immunochromatographic test strip and a preparation method and application thereof. The colloidal gold immunochromatographic test strip comprises a colloid strip. The colloid strip comprises a sample pad, a colloidal gold pad, a nitrocellulose membrane and water-absorbing filter paper which are sequentially overlapped. A gold-labeled cryptococcal antibody is embedded in the colloidal gold pad, the nitrocellulose membrane is coated with a cryptococcal antibody as a detection line, is also coated with an antibody against a virus antibody on colloidal goldas a control line. The colloidal gold immunochromatographic test strip has good sensitivity, specificity, repeatability and stability, has a high recovery rate for a target compound, and can providemore accurate and reliable test results.

The invention discloses a primer, molecular beacon and kit for fast detecting various clinically common fungi and identifying strains and belongs to the technical field of microbiological detection. The sequences of the primer is as shown in SEQ ID No. 1-4, and the sequences of the detecting molecular beacon is as shown in SEQ ID No. 5-7. The primer and the molecular beacon have the advantages that the specific primer and the molecular beacon are designed at conserved sites by comparing the gene sequences of various clinically common fungi, and the primer and the molecular beacon can fast detect and identify 8 clinically common candida, 1 cryptococcus, aspergillus, mucor and penicillium. The kit for detecting various fungi contains the primer and the molecular beacon and can fast and accurately detect various clinically common fungi.

The invention relates to application of chenopodium vulvaria volatile oil to bacteriostatic / bactericidal and antioxidative products. The chenopodium vulvaria volatile oil can be obtained by a water steam distillation or CO2 supercritical extraction method, and main ingredients of the chenopodium vulvaria volatile oil are identified by gas chromatograph / mass spectrum. The chenopodium vulvaria volatile oil has an obvious effect of killing or inhibiting bacteria such as staphylococcus, streptococcus, enterobacter, salmonella, shigella, pseudomonas, bacillus, escherichia coli and the like and fungi such as candida, cryptococcus, penicillin, aspergillus, mucor, microsporon, trichophyta, epidermophyton and the like. A diphenyl picryl phenylhydrazine (DPPH) method proves that the chenopodium vulvaria volatile oil has the high antioxidant activity, can be widely applied to industry of food, medicines, cosmetics, health-care products and the like, and is used for preparing the bacteriostatic / bactericidal and antioxidative products.

The compositions and methods described herein discloses the design, synthesis and testing of compounds that act as inhibitors of DHFR. The basic scaffold of these inhibitors includes a 2,4-diaminopyrimidine ring with a propargyl linker to another substituted aryl, bicyclo or heteroaryl ring. These DHFR inhibitors are potent and selective for many different pathogenic organisms, including the DHFR enzyme from bacteria such as Bacillus anthracis and methicillin-resistant Staphylococcus aureus, fungi such as Candida glabrata, Candida albicans and Cryptococcus neoformans and protozoa such as Cryptosporidium hominis and Toxoplasma gondii. These compounds and other similar compounds are also potent against the mammalian enzyme and may be useful as anti-cancer therapeutics.

The invention relates to an anti-cryptococcal capsular polysaccharide hybridoma cell strain and an anti-cryptococcal capsular polysaccharide monoclonal antibody secreted thereof. The antibody can be specifically bound to cryptococcal capsular polysaccharide and can be used for in vitro detection of cryptococcal infection. The antibody titer is up to more than one million, and the antibody has excellent affinity and good specific binding capability. The detection limit of a colloidal gold-labeled immunodiagnostic reagent developed with the antibody as the raw material is 0.5 ng / ml, and the antibody has excellent performance in sensitivity, specificity, stability and all other aspects.

The invention provides an oligosaccharide vaccine for preventing invasive fungal infection. The oligosaccharide vaccine comprises chitosan oligosaccharide coupled with an immunogenic carrier protein;the mass ratio of the immunogenic carrier protein to the chitosan oligosaccharide is 0.7-210: 1; the chitosan oligosaccharide has a deacetylation degree of 0.05-98% and an average relative molecular weight smaller than 5000 Da; and the immunogenic carrier protein is a non-human protein. The oligosaccharide vaccine provided by the invention is capable of effectively activating Th17 and Th1 cellularimmunity through a proper acetylation degree so as to prevent broad-spectrum fungal infections. The oligosaccharide vaccine is capable of treating or preventing invasive fungi such as candida albicans, aspergillus, cryptococcus and pneumocystis jeroveci.

It is intended to provide novel compositions usable in improving the flavor of an alcoholic beverage made from grapes typified by wine. Namely, a composition usable in improving the flavor of an alcoholic beverage made from grapes which contains the culture of a strain belonging to a genus Aspergillus, Penicillium, Rhizopus, Rhizomucor, Talaromyces, Mortierella, Cryptococcus, Microbacterium, Corynebacterium or Actinoplanes and being capable of producing diglycosidase.

The invention relates to a soil-environment microorganism disinfectant and a preparing method thereof. The disinfectant is a liquid fertilizer type soil disinfectant prepared from inorganic nutrient substances and microorganism cenobium capable of removing soil toxins in a combined mode. According to the disinfectant and the preparing method, five kinds of bacteria which have bacterial strains such as cryptococcus humicolus, lactobacillus acidophilus, coralline Nocardia amarae, actinomycetes agrobacterium tumefaeiens and trichoderma koningii and have ingredients capable of powerfully removing toxic pollutants in soil are screened out; test surveying indicates that after the microorganism disinfectant is applied, 85% to 90% of remaining hazardous substances such as pesticides can disappear in 30 days to 50 days, and the remaining hazardous substances can be completely removed after the microorganism disinfectant is continuously applied several times.

The invention discloses a cryptococcus detection card. The card comprises a rubber plate, wherein a sample pad, a gold mark pad, a chromatographic film and a first water absorption pad are sequentially arranged on the rubber plate end to end along the sample flowing direction; a second water absorption pad is arranged between the chromatographic film and the rubber plate; a detection area and a quality control area are arranged on the chromatographic film; and a colloidal gold marked first monoclonal antibody for resisting cryptococcus capsular polysaccharide is adsorbed on the gold mark pad.The detection card or detection box can be adopted to quickly and sensitively detect the existence of cryptococcus capsular polysaccharide antigen, and determine whether a detected object is affectedby cryptococcus or not. The detection card or detection box has high sensitivity, strong specificity and high detection speed, can give a detection result in five minutes, and is simple in operation.

The invention relates to a method for biologically synthesizing sclareolide. The method comprises the steps of taking a yeast culture medium and separately adding sclareol and a light white cryptococcus protoplast fluid into the yeast culture medium to obtain a mixture, wherein the sclareol accounts for 3-8% of the total mass of the yeast culture medium, and the light white cryptococcus protoplastfluid accounts for 4-10% of the total mass of the yeast culture medium; placing the mixture in a glass vessel, and then shaking the vessel for fermentation for 10 days on a shaking table at the temperature of 23-28 DEG C and the speed of 140-180 r / min to obtain sclareolide. According to the method, a microorganism conversion method is utilized, and sclareol is used as a substrate and converted bylight white cryptococcus to prepare sclareolide, so that the problems that a synthesis technology process of sclareolide based on a chemical method is environmentally unfriendly, complicated in step,low in safety and the like are solved, and a new way is provided for synthesizing sclareolide.

The invention discloses a C2H2-type transcription factor gene asr1. The C2H2-type transcription factor gene asr1 is cloned in a Cryptococcus humicolus BSLL1-1 strain, has a nucleotide sequence shown in the formula of SEQ ID NO: 1 and codes a protein with an amino acid sequence shown in the formula of SEQ ID NO: 2. An asr1 recombinant fragment blocked by a hygromycin gene is introduced into Cryptococcus humicolus, the Cryptococcus humicolus produces mutation, and the mutant strain increases resistance to various ionic stresses and has the application potential to reduce various ions in soil.

The invention discloses a novel human antimicrobial peptide LL-37 derivative and an application thereof. The amino acid sequence of human antimicrobial peptide LL-37 derivative is shown as SEQ ID NO:1. The invention further comprises an application of the novel human antimicrobial peptide LL-37 derivative in preparing antimicrobial agents. The antimicrobial agents are antibacterial drugs or anti-fungal drugs. The bacteria or fungi are at least one of cryptococcus, histoplasma capsulatum, monilia albicans, staphylococcus aureus, streptococcus pneumoniae, escherichia coli and salmonella. Compared with the prior art, the human antimicrobial peptide LL-37 derivative with the sequence, provided by the invention, has the advantages of high stability, excellent antimicrobial effect and the like,and has a wide application prospect in the field of antimicrobial drug preparation.

The invention discloses a microbial preparation efficiently converting heavy metal cadmium in contaminated soil and a preparation method thereof. The microbial preparation is obtained by mixing and conducting enrichment cultivation on a flora 1 composed of candida, phialemonium, rhodosporidium toruloides and cryptococcus and a flora 2 composed of acidithiobacillu thiooxidans, acidithiobacillus caldus, leptospirum ferrooxidans, sulfobacillus thermosulfidooxidans, acidithiobacillus ferrooxidans and ferroplasma thermophilum. By using the microbial preparation for treating the cadmium contaminated soil, after the metal cadmium is converted to be a soluble state from an insoluble state, the removal rate is 70-82%, and the microbial preparation has the advantages of being high in conversion efficiency, short in cycle, low in cost, free of secondary pollution, easy to popularize and the like.

The invention provides a gene chip for detecting common pathogenic bacteria in cosmetics. The gene chip comprises a solid phase carrier and an oligonucleotide probe fixed on the solid phase carrier, wherein the oligonucleotide probe comprises DNA segments which are selected from 16S-23SrDNA intermediate zones of micrococcus luteus, staphylococcus aureus, streptococcus pyogenes, salmonella, proteus mirabilis, citrobacter freundii, pseudomonas aeruginosa, enterobacter aerogenes and providencia stuartii and 18S-28SrDNA intermediate zones of the ipaH gene of shigella, candida albicans and cryptococcus neoformans, or complementary DNA or RNA sequences of the DNA segments. The invention also provides a kit for detecting the common pathogenic bacteria in the cosmetics by use of the gene chip. The gene chip and the kit can be used for detecting the common pathogenic bacteria in the cosmetics, and are simple and convenient to operate, high in accuracy and excellent in repeatability.

The invention discloses an application of a microbial preparation for efficiently converting heavy metal cadmium in polluted soil. The microbial preparation is prepared by performing enriched cultivation on the following two mixed floras: (1) candida mycoderma bacteria, aspergillus monicus, rhodosporidium toruloides and cryptococcus which form a flora 1; and (2) acidithiobacillus thiooxidans, leptospirillum ferrooxidans, acidithiobacillus caldus, sulfobacillus thermosulfidooxidans, acidithiobacillus ferrooxidans and thermos ferric bacteria which form a flora 2. By using the microbial preparation to treat the cadmium polluted soil, the metal cadmium is converted from being indissolvable into being soluble and the removal rate is 70-82%. The microbial preparation disclosed by the invention has the advantages of being high in conversion efficiency, short in period, low in cost, free of secondary pollution, easy to popularize and the like.