119 results about "Lavage fluid" patented technology

The present invention relates to closed catheter suction systems used to aspirate secretions from the trachea of a patient and to rinse the catheter after such aspiration. The apparatus comprises a flexible suction catheter encased within a sheath that is fixed to a vacuum source at its proximal end and extendable through an adapter assembly at its distal end, near the patient. The adapter assembly connects the closed catheter suction system to a patient connector, and a suction control valve at the proximal end operates to aspirate secretions and to rinse the catheter. The closed catheter suction system also includes a lavage port and a rinse port, the latter of which is positioned near the distal end and is isolated from the ventilation circuit through the innovative use of a rinse chamber and a one-way valve. Advantageously, the lavage port is positioned near the proximal end, away from the patient, so as to prevent the accidental leakage of lavage into the ventilation circuit. Further, both the lavage port and the rinse port include a self-closing fill-valve that prevents the accidental loss of ventilator volume and which also prevents the discharge of infectious pathogens to the surrounding environment throughout the aspiration and rinse procedures.

A portable, lightweight bowel evacuation apparatus and method of delivering a colonic lavage is provided. The apparatus includes a liquid bag containing lavage liquid connected to a supply conduit that leads to a nozzle assembly. The apparatus also includes a drain conduit leading from the nozzle assembly to a waste collection. A pulsating member, such as a manual pump or squeeze bulb is provided to generate pulsed waves in the lavage liquid in the patient's colon so as to break up and remove fecal material.

A hand-held disposable body cavity irrigation device comprises a pump with an inlet and an outlet, a container for lavage fluid fastened removably to said pump, an applicator having an insertion tip, and divided longitudinally into an injection chamber with a first fitting and a drain chamber with a second fitting, and a first tube connecting said outlet to the first fitting wherein the first tube comprises at least one malleable element embedded longitudinally into an elastic wall, said inlet communicates with the lavage fluid within the container, and the drain chamber is disposed predominantly below the injection chamber and has at least one vent beyond the insertion tip.

The invention relates to a kit for detecting novel coronavirus N protein and an application of the kit. The kit comprises (1) one antibody capable of being bound to the novel coronavirus N protein, and (2) the other antibody marked by a marker and capable of being bound to the novel coronavirus N protein when the novel coronavirus N protein can be bound to (1) to limit the antibody. The using method of the kit comprises the step of determining whether a patient suffers from the novel coronavirus pneumonia or not by detecting the level of the novel coronavirus N protein in an individual sample.The kit for detecting the novel coronavirus N protein provided by the invention can be used for detecting the coronavirus N protein in nasopharynx swabs, oropharynx swabs, alveolar lavage fluids, serum, plasma, whole blood, sputum, oral swabs or saliva samples so as to be used for diagnosing novel coronavirus pneumonia.

The invention relates to an immunochromatographic kit for rapidly detecting novel coronavirus N protein as well as a preparation method and application of the immunochromatographic kit. The immunochromatographic kit comprises a test strip, the test strip comprises a detection line and a quality control line, the detection line is coated with an anti-novel coronavirus N protein antibody, and the quality control line is coated with a goat anti-rabbit polyclonal antibody. The immunochromatographic kit for rapidly detecting the novel coronavirus N protein provided by the invention can be used fordetecting the novel coronavirus N protein in a nasopharynx swab, an oropharynx swab, an alveolar lavage fluid, an oral swab or a saliva sample so as to be used for diagnosing novel coronavirus pneumonia.

The invention provides a kit for detecting SARS-CoV-2 novel coronavirus nucleic acid at a constant temperature by using an enzyme digestion probe. The kit comprises an isothermal amplification primerfor detecting an ORF1ab gene of SARS-CoV-2 novel coronavirus and a corresponding RNA-containing base probe capable of being digested by RNase H, wherein the left end and the right end of an RNA base of the base probe are respectively marked with a reporter fluorophore and a quencher. By utilizing the efficient isothermal amplification primer and the probe, constant-temperature multiple detection can be carried out on the SARS-CoV-2 novel coronavirus, and the kit has high sensitivity and high specificity. The novel coronavirus nucleic acid detection kit disclosed by the invention has high sensitivity and specificity, can be used for detecting throat swabs, alveolar lavage fluid and sputum samples of suspected novel coronavirus pneumonia cases, suspected aggregated case patients and other patients needing novel coronavirus infection diagnosis or differential diagnosis, and has the characteristics of simplicity, convenience, quickness and accuracy.

A safe, simple, convenient, low-pollution and good-repeatability extracting method of alveolar lavage fluid metagenome DNAs is provided. The method includes steps of liquefying a sample, crushing cells, isolating target DNAs, removing impurity proteins and salt ions, performing DNA adsorption and performing DNA elution. The method can increase the yield and purity of the metagenome DNAs and can meet PCR, DNA library construction, gene sequencing, and other molecular biological experiments.

The invention discloses a new application of nucleotide and an application of the nucleotide in preparation of drugs for preventing and treating diseases such as allergic rhinitis or functional food for relieving symptoms of the allergic rhinitis. The nucleotide is a mixture of five nucleotides, namely CMP, AMP, UMP, GMP and IMP. The molecular weight of the nucleotide is small, absorption of the nucleotide taken by a human body is fast, and bioavailability of the nucleotide is high. It is found that the ingestion of the nucleotide can relieve the symptom of allergic rhinitis of mice, relieve splenomegaly caused by allergy, significantly reduce the histamine level in serum and nasal cavity lavage fluid, and effectively regulate inflammatory factors, thereby playing a role in treating allergic diseases. The nucleotide can be used for preparing medicines for preventing and treating diseases such as allergic rhinitis or functional foods for relieving symptoms of allergic rhinitis, and provides a new way for regulating the immune function in a diet intervention mode.

The invention discloses a multi-amplification and high-throughput sequencing method and kit for identifying pathogenic microorganisms, and the method comprises the following two steps: in the first-step amplification, an upstream long primer pool is used for single-end amplification; in the second-step amplification, a downstream long primer pool, a primer P1 and a primer P2 are added into a reaction system, the three primers form a primer for the second-step amplification, and then quality control, purification, high-throughput sequencing and data analysis are carried out. According to the method, the pathogenic microorganisms which cannot be identified in a traditional clinical method are detected in a human respiratory tract alveolar lavage fluid sample and a blood or plasma sample by using a two-step multiple amplification and next-generation sequencing method for the first time, the sensitivity is high, the detection limit is 50 pathogenic genome copies per milliliter, the method is rapid and simple, the sequencing data volume is small, and the detection cost is reduced.

The invention discloses a method for preparing a pneumonia rat model and a model evaluation method, and belongs to the technical field of biology. The method for establishing the pneumonia rat model is non-invasive and safe to rats, does not damage other organs of rats in the whole process, and effectively avoids the death of the rats. Besides, the method has simple operation and high success rate. According to the pneumonia rat model established by the method, pseudomonas aeruginosa in bronchoalveolar lavage fluid is obviously increased, inflammatory factors IL-4 and TNF-alpha are obviously increased, the level of adhesion factor IFN-gamma is obviously reduced, and the number of leukocytes is obviously increased. Meanwhile, the lung index is also obviously increased, which indicates thatthe model is established well.

The invention provides a sample harvester of bronchoalveolar lavage fluid, which comprises a fluid collecting bottle, a first pipeline, a second pipeline, a first buckling cover and a second bucklingcover; there are multiple liquid collecting bottles, and each liquid collecting bottle is detachably and hermetically mounted at the bottom of the first buckling cover through a bottle opening of theliquid collecting bottle; the first pipeline comprises a main pipeline and a plurality of branch pipelines, and the main pipeline is communicated with a plurality of branch pipelines; the first buckling cover is provided with first through holes and a second through hole, the number of the first through holes and the liquid collecting bottles are the same; each branch pipeline is connected with one first through hole, and the second buckling cover is connected with the first buckling cover, and the second buckling cover can move relatively to the first buckling cover; a third through hole is formed in the second buckling cover, and when the second buckling cover and the first buckling cover move relatively, the third through hole can be communicated with each second through hole; one end of the second pipeline is connected with the third through hole. The sample harvester can collect multi-tube bronchoalveolar lavage fluid samples in one time without replacing tubes, the safety is extremely high, and meanwhile the operation is easy and convenient.

The invention relates to a real-time fluorescence RPA detection kit for aspergillus fumigatus as well as a special primer and a probe of thereof, and particularly provides a primer composition for RPAdetection, the primer composition comprises a forward primer and a reverse primer, the forward primer has a nucleotide sequence shown as SEQ ID NO: 1, and the reverse primer has a nucleotide sequenceshown as SEQ ID NO: 2. The primer composition for RPA detection can be used for rapidly, conveniently, efficiently, specifically and sensitively detecting aspergillus fumigatus components in samplesto-be-detected, such as thalli, sputum, blood and bronchoalveolar lavage fluid, under an isothermal condition.

The invention relates to application of Faecalibacterium prausnitzii in relieving allergic asthma and rhinitis Th2 reaction, and belongs to the technical field of microorganisms and medicines. According to the application disclosed by the invention, the Faecalibacterium prausnitzii A2-165 is applied to relieving of allergic asthma and rhinitis, and the application is specifically embodied in that(1) the infiltration effect of memory inflammatory cells with lung pathological symptoms of mice with allergic asthma and rhinitis is remarkably improved; (2) the contents of IL-4, IL-5 and IL-13 in alveolar lavage fluid for the mice with allergic asthma and rhinitis are remarkably reduced; (3) the generation of dust mite specific immunoglobulin IgG1 in serum of the mice with allergic asthma and rhinitis is remarkably inhibited; and (4) the ratio of Tregs in the spleen is remarkably increased, so that the Faecalibacterium prausnitzii A2-165 has a huge application prospect in the preparation ofa product for prevention and/or treatment.

The invention discloses a novel preservation solution for an alveolar lavage fluid microbial nucleic acid. The preservation solution comprises a treatment solution I and a treatment solution II, wherein the treatment solution I comprises a guanidine hydrochloride buffer solution, a nuclease inhibitor, a metabolism inhibitor, an alkali metal halide and glycerol, and the treatment solution II comprises an alkali metal hydroxide and a surfactant. The treatment solution I and the treatment solution II which are separately stored are used as the novel preservation solution for the alveolar lavage fluid microbial nucleic acid, so that the preservation solution can be prevented from generating precipitates, at the same time, different interference impurities can be treated respectively in a targeted manner, alveolar lavage fluid samples can be rapidly stabilized, the activity of nuclease is effectively inhibited, the cell lysis is prevented, at the same time, the free nucleic acid in the alveolar lavage fluid can be well protected, the preservation time is long, the accuracy and convenience of nucleic acid detection are improved, and thereby the preservation solution has great applicationvalue in the detection and diagnosis of tumor diseases, viruses and the like.

The invention belongs to the field of gene diagnosis, and relates to application of a detection reagent using methylation of HOXA7 as a detection object to preparation of a lung cancer diagnosis reagent. The detection reagent uses HOXA7 as a tumor marker for detecting the lung cancer, reaches high sensitivity of 88.6% and high specificity of 95% in sputa, reaches high sensitivity of 76.2% and highspecificity of 95% in lavage fluid, and is higher in detection sensitivity than existing reported tumor markers of the lung cancer; particularly for adenocarcinomas, sensitivity of the detection reagent is greatly promoted; and the detection reagent has great application value for detection and diagnosis on the lung adenocarcinomas.

The invention relates to a cell preservation solution for rapid cell immunohistochemistry as well as a preparation method and application of the cell preservation solution. The cell preservation solution provided by the invention is mainly composed of a buffer solution, an erythrocyte lysis reagent, an osmotic pressure maintaining agent, a cell morphology maintaining reagent, a fixing agent, a preservative, a mucus treatment reagent, an anti-aggregation reagent and a reagent for cell immunohistochemical cell antigen treatment. The reagent for cell immunohistochemical cell antigen treatment comprises formalin and zinc salicylate, and the formalin and the zinc salicylate cooperate with each other to effectively catalyze an immunohistochemical reaction, so that cell antigens do not need to berepaired, the reaction time of cell immunohistochemical detection is shortened, the detection efficiency is improved, and the timeliness of pathological diagnosis is enhanced. The cell preservation solution provided by the invention can be applied to preserving or collecting various cell samples such as cervical exfoliated cells, pleural effusion, alveolar lavage fluid, cerebrospinal fluid, sputum and urine. Meanwhile, the invention provides a preparation method of the cell preservation solution .

The invention relates to a sample containing a gastric mucosa lavage fluid collected from a subject who has received a gastric mucus removal treatment and a method for detecting a disease-related marker using the same. By using the sample of the invention, the disease-related marker can be detected conveniently, less invasively, highly sensitively and highly accurately.

The invention discloses an oxygenated separation type bronchoalveolar lavage fluid collection device. The device includes a lavage tube, a normal saline container, a control device and a collection container; normal saline is sprayed out from an opening of the tube and side wall holes, multi-directional stereoscopic extrusion flushing is achieved, the flushing effect is enhanced, a pressurizing valve is a one-way pressurizing outlet valve, flushing and recycling are independently completed, and backflow of the normal saline does not occur; a reducing valve is used for buffer decompression of received oxygen, the pressure of oxygen is prevented from being large, and oxygen can be safely used by patients; a piston in a collection container is recycled through manual control, collected lavagefluid is filtered through a filter net, impurities are effectively removed, the bottom of the collection container is connected with a liquid collection funnel, collection of lavage fluid samples ata fluid outlet is controlled through a fluid outlet switch, in the collection process, multiple-sample collection can be directly conducted, sampling is convenient, and operation is simple.

The invention relates to a novel lavage solution preparation, which is composed of the following components in mass percentage: 3.5-6.2% of sodium hyaluronate, 2.0-5.0% of povidone-iodine, 8.0-12.5% ​​of nonoxynol, and 3.2% of chitosan ‑5.0%, phosphate buffer 50.0‑75.0%, and the balance is water. The inventor conducted a large number of screening experiments on components and dosages in the early stage, and unexpectedly found that the technical solution of the present invention can effectively prevent the form of tissue adhesion, promote wound healing and can It can effectively stop bleeding, and can also play a role in sterilization, and has an excellent inhibitory effect on microorganisms. It is widely used in ophthalmology, surgery, abdominal chemotherapy and other operations, and has broad application prospects.