48 results about "Lavage fluid" patented technology

The invention relates to the technical field of pathogen detection, in particular to a primer probe combination and a kit for detecting various invasive fungi and application of the primer probe combination and the kit. According to the primer and probe combination provided by the invention, a digital PCR (Polymerase Chain Reaction) platform technology is utilized, so that the rapid, efficient, accurate, sensitive and high-specificity simultaneous quantitative detection of Aspergillus fumigatus, Aspergillus, Mucoriums, Cryptococcus and Pneumocystis jirovecii is realized. The rapid detection of the common invasive fungi in sputum, alveolar lavage fluid, urine, blood and other samples is realized, the detection process does not need culture, the detection period is short, the manpower is greatly reduced, and the detection efficiency is improved.

The invention discloses an application of isothunberine or a pharmaceutically acceptable salt thereof in preparation of a medicine for preventing and / or treating asthma diseases. It is found that in an OVA-induced mouse asthma model, the isothunberine significantly reduces airway resistance excited by acetylcholine, improves inflammatory cell infiltration and airway mucus high secretion of asthma model mouse lung tissue, reduces the levels of lgE and OVA-lgE in serum and related inflammatory factors in alveolar lavage fluid, and has the effect of preventing and treating asthma. Therefore, the effect of treating asthma is achieved. Therefore, the isothunberine has the potential of preventing or treating the asthma, provides a new treatment mode for the treatment of the asthma, and has a wide application prospect.

The invention discloses a broncholung lavage fluid composition for treating non-small cell lung cancer, and relates to the technical field of cancer treatment. The preparation method of the bronchus lung lavage fluid composition for treating the non-small cell lung cancer comprises the following steps: S1, performing metagenome sequencing on a BALF sample, S2, grouping by R / NR based on differential strains obtained in S1 to obtain core bacteria, performing single-strain Kaplan-Meier survival analysis on the strains, and determining the survival rate of the single-strain Kaplan-Meier to obtain the bronchus lung lavage fluid composition for treating the non-small cell lung cancer. Finally, seven key microorganisms, namely bronchopulmonary lavage fluid combinations, which are remarkably related to immunotherapy PFS are locked; s3, determining the relationship between the seven key BALF bacteria and the existing immune therapeutic effect marker; and S4, verifying the generation conditions of IL6, IL8 and RANTES cytokines promoted by the seven strains through an in-vitro cell experiment, and revealing the activation and enhancement effects of the seven strains on immune cell functions. According to the invention, the immunotherapy effect of the non-small cell lung cancer patient can be promoted to be improved by combining a plurality of beneficial specific microorganisms in an airway administration mode. In conclusion, the method has a good application prospect.

The utility model relates to the technical field of drainage tubes, and discloses a sandwich drainage tube which comprises an outer tube and an inner tube, one end of the inner tube is located on the inner side of the outer tube, the other end of the inner tube penetrates through the side wall of the other end of the outer tube and is fixedly connected with the outer tube, and a plurality of lavage channels are communicated between the end, located in the outer tube, of the inner tube and the outer tube. A plurality of leading-out holes and a plurality of leading-in holes are formed in the outer wall of the end, close to the lavage channels, of the outer tube in a penetrating mode, the leading-in holes are communicated with the lavage channels respectively, and the leading-out holes are located between every two adjacent lavage channels respectively. According to the sandwich drainage tube, the inner cavity of the inner tube is isolated from the inner cavity of the outer tube through the lavage channel, so that liquid for lavage into the body of a patient through the inner tube directly enters the body of the patient through the guide-in hole, the lavage liquid is prevented from being directly guided out by the outer tube, the lavage efficiency is improved, and the lavage treatment burden of the patient is relieved.

The invention belongs to the technical field of biomedicine, and discloses an antibody for evaluating the curative effect of infantile pneumonia asthma and application of the antibody. The heavy chain CDR sequence of the monoclonal antibody (secreted by the hybridoma cell strain 2B5) is SEQ ID NO: 2-4, the light chain CDR sequence of the monoclonal antibody (secreted by the hybridoma cell strain 2B5) is SEQ ID NO: 6-8, the dissociation constant of the monoclonal antibody and an IL-5R antigen reaches 2.435 * 10 <-1 > / L, and the affinity is high. The antibody can accurately evaluate the curative effect of infantile pneumonia and asthma by detecting the lung function, the ratio of eosinophilic granulocytes in bronchoalveolar lavage fluid and lung tissue inflammation, can also significantly improve airway obstruction and inflammation of asthma model mice, has an effect superior to that of dexamethasone, solves the problems that existing curative effect evaluation is lack of specific tools and has large side effects of therapeutic drugs, and has good clinical application prospects. And a new scheme is provided for curative effect evaluation and adjuvant therapy of infantile pneumonia asthma.

The invention discloses application of irisin in treatment of acute lung injury / acute respiratory distress syndrome. The research finds that the plasma irisin level is obviously reduced in ARDS patients, and the survival prognosis of the patients with the lower irisin level is poorer. Neutrophil as the most inflammatory cells is a cause for promoting lung tissue injury. Further analysis finds that the level of irisin is in negative correlation with the number of circulating neutrophils, and irisin incubation can significantly reduce in-vitro survival promoting (apoptosis delay) phenotypes of neutrophils in ARDS mouse alveolar lavage fluid. Iris receptor integrin alphaVbeta5 inhibitor treatment can promote neutrophil infiltration and aggravate an ARDS process. The invention provides a novel therapeutic drug and strategy for the treatment of acute lung injury / acute respiratory distress syndrome.

The invention relates to the technical field of medical lavage equipment, and discloses a lung lavage flushing and sucking machine which comprises an adjustable water inlet pump for providing lavage fluid input power; one end of the water inlet pipe is connected with the adjustable water inlet pump, and the other end of the water inlet pipe is inserted into the lung through a trachea cannula and is provided with a water injection nozzle; one end of the water outlet pipe is close to the lung to collect waste liquid after lavage, and the other end extends to the far end; the wastewater discharge structure is connected with one end, extending to the far end, of the water outlet pipe and used for receiving wastewater after irrigation. The lavage force is improved through the adjustable water inlet pump, efficient lavage of the deep part in the lung is achieved, meanwhile, continuous collection of waste water is achieved through cooperation of the water outlet pipe and the waste water collecting device, and the lavage efficiency in the lung is improved.

The invention provides a spanishneedles herb exosome as well as a preparation method and application thereof in acute lung injury, and belongs to the technical field of biological medicines. According to the preparation method of the spanishneedles herb exosome provided by the invention, the spanishneedles herb exosome can be extracted from a plant source through a simple method. In one embodiment of the invention, a lung injury model constructed by using LPS is used as an experimental animal to verify the therapeutic effect of the spanishneedles herb exosome. Results show that after the spanishneedles herb exosome is used for treatment, mouse lung tissue injury and inflammatory cell exudation can be relieved, and lung injury caused by LPS can be relieved; tNF-alpha and IL-1beta in lung lavage fluid of a mouse are remarkably reduced, and lung inflammation infiltration of the mouse can be relieved. Therefore, the spanishneedles herb exosome has the effect of treating the acute lung injury and can be used for preparing the medicine for treating the acute lung injury.

The invention relates to the technical field of biomedicine, in particular to a group of key biomarkers for predicting endometrial receptivity, including specific microbial strains and metabolites, and a prediction model containing the markers. The method comprises the following steps: collecting a uterine cavity lavage fluid sample, carrying out 16S rRNA gene sequencing and non-targeted metabonomics analysis, and screening out eight key microbial strains and eight key metabolites; based on the markers, the following diagnostic models are constructed: a model only based on eight microbial strains; a model based on only eight metabolites; a combined microorganism and metabolite model; and a clinical data model is further combined. According to the method, a microbiome and metabolome multi-omics conjoint analysis strategy is adopted, strict screening is carried out in combination with LASSO regression, SVM-RFE and other machine learning algorithms, and the obtained biomarker is high in specificity. The constructed model, especially a combined model, shows high prediction precision and good calibration degree through verification of various curves.

This invention discloses the application of a peptide SRD in the preparation of drugs for treating acute lung injury, belonging to the field of biomedical technology. In vivo experiments verified the protective effect of peptide SRD on acute lung injury (ALI), particularly its effect on improving AT2 mitochondrial function. In animal models of ALI, tail vein injection of peptide SRD significantly reduced the protein concentration and total cell count in bronchoalveolar lavage fluid, significantly alleviated the severity of lung injury, lowered histological scores, significantly decreased SOD2 acetylation levels while significantly increasing total SOD2 protein levels, and significantly increased SPC levels (an indicator of AT2 survival). ELISA analysis showed a significant decrease in the levels of IL-6, IL-1β, and TNF-α, indicators of pneumonia in mice. These experimental results confirm that the antioxidant peptide SRD can improve oxidative stress damage in ALI, effectively reduce AT2 cell damage under ALI conditions, promote AT2 regeneration and repair, effectively improve lung function, and reduce the inflammatory response of damaged lungs, thus exhibiting a good therapeutic effect on ALI.

The utility model provides a catheter device for subsection bronchial lavage, which comprises an inner catheter for lavage fluid injection and suction and a mesh basket mechanism for plugging and positioning, and the mesh basket mechanism is arranged on the outer side of the front end of the inner catheter; the mesh basket mechanism comprises a mesh basket body located on the inner side and a film sleeve located on the outer side, and the mesh basket body and the film sleeve are arranged in a stacked mode in the radial direction of the inner catheter. The mesh basket body is formed by weaving a plurality of filaments, and the filaments at least comprise shape memory alloy wires. The utility model can realize the positioning and plugging effect at the specified position in the subsection bronchial tube, the mesh basket mechanism automatically opens to achieve the plugging function, and the utility model has the advantages of convenient operation and strong practicability.

The application discloses a kind of nanoparticles for removing lung mucus and its preparation method and application, it is related to the field of biological medicine.The nanoparticles of the application are composed of inner core and shell coated on the surface of the inner core, wherein the inner core contains citric acid and photoluminescent polymer-aliphatic polyester copolymer;The shell contains deoxyribonuclease and hydrophilic polymer.The nanoparticles provided by the application have excellent biocompatibility and multifunctional integration characteristics, which realizes effective penetration of lung mucus barrier, specific degradation of DNA network, down-regulation of pro-inflammatory factors and inhibition of mucus production in sequence through cascade response mechanism, breaking through the limitations of traditional single therapy.In addition, the in vivo experimental results show that it can significantly reduce the thickness of lung mucus and the protein concentration of lavage fluid, and effectively relieve airway obstruction, and can be used for removing lung mucus.

The present application provides a kind of respiratory tract sampler for collecting avian tracheal lavage fluid, belong to the technical field of poultry respiratory tract sampling instrument, including support part, flexible catheter part, positioning and fixing assembly, elastic catheter head assembly, elastic sliding sleeve assembly and inner integrated tube assembly;Support part opens poultry beak, flexible catheter part extends into trachea, insertion cylinder is recycled in head connection pipe, elastic fin supports flexible fin, avoids bending and reduces mucosa irritation;After reaching the predetermined depth, inner integrated tube assembly provides positive pressure gas, drives insertion cylinder to extend, makes limit inclined table and clamping inclined table self-locking, through-hole is exposed in trachea, positive pressure simultaneously provides oxygen for a short time;Subsequently switch to lavage mode, lavage fluid is washed trachea through through-hole, then switch negative pressure back suction to collect sample;After use, move sliding sleeve, manually push and press flexible head to make insertion cylinder reset, it is convenient to disinfect and reuse;Can reduce poultry discomfort, accurately control lavage position, realize oxygen supply, lavage, back suction multi-functional switching.

The present application relates to the technical field of medical apparatus and instruments, in particular to a bronchoscope alveolar lavage fluid collector, which is applied to a bronchoscope alveolar lavage fluid suction system, the suction system comprises a bronchoscope unit, a waste liquid collecting unit and a negative pressure aspirator, and the bronchoscope unit, the collector, the waste liquid collecting unit and the negative pressure aspirator are connected in series, the collector comprises a valve mechanism, an adapter mechanism and a specimen jar; the specimen jar is detachably installed on the adapter mechanism. The valve mechanism is communicated with the bronchoscope unit and the waste liquid collecting unit respectively, and the adapter mechanism and the detachable assembly structure of the specimen jar are matched, when sampling, the specimen jar is only needed to be installed on the side of the valve mechanism by using the adapter mechanism, and a complete sampling channel can be constructed, after sampling, the sampling channel can be switched to a waste liquid collecting channel by only removing the adapter mechanism, and the pipeline between the bronchoscope unit and the waste liquid collecting unit does not need to be repeatedly detached and connected, so that the pipeline pollution risk and the operation complexity are reduced.

The invention belongs to the technical field of biological medicines, and discloses application of AC1903 in preparation of drugs for treating and preventing neutrophil asthma. The invention provides the application of the TRPC5 inhibitor (especially AC1903 or pharmaceutically acceptable salt thereof) in preparation of drugs for preventing and / or treating asthma (especially neutrophil asthma) for the first time. Experiments prove that by intraperitoneal injection of AC1903, the wheezing symptom of a TDI asthma mouse can be remarkably relieved, the airway hyperreactivity, the airway surrounding inflammation level, the total number of inflammatory cells in the airway and the neutrophil count of the TDI asthma mouse are relieved, and secretion of type-2 cytokines and type-17 cytokines of alveolar lavage fluid of the asthma mouse is inhibited. The traditional Chinese medicine composition has a good treatment effect on asthma and has no obvious toxic or side effect.

The invention discloses a child colon lavage device and relates to the technical field of clinical nursing. The device comprises a liquid storage tank, a liquid inlet transmission box, a fluid driving part, a liquid outlet transmission box and a negative pressure generating part, the liquid inlet transmission box is provided with a liquid inlet cavity, the liquid inlet cavity is provided with a first liquid inlet and a first liquid outlet, the first liquid inlet is communicated with the liquid storage tank through a first pressure difference one-way valve, and the first liquid outlet is communicated with a liquid inlet pipe through a second pressure difference one-way valve; the fluid driving part and the negative pressure generating part are in transmission connection, when the fluid driving part generates extrusion force, the negative pressure generating part synchronously moves to generate adsorption force, the liquid inlet process of lavage liquid and the liquid discharging process of lavage backflow liquid form mechanical linkage, the extrusion force and the flow speed of the inlet liquid are matched with the negative pressure adsorption force and the suction rate of the discharged liquid, and therefore the suction efficiency of the lavage liquid is improved. The amount of lavage fluid injected into the intestinal tract can be matched with the amount of sucked backflow fluid in real time, and the risks of abdominal distension, abdominal pain, intestinal mucosa injury and perforation caused by deposition of a large amount of lavage fluid are avoided.

The present application relates to the technical field of gastric lavage machine, and disclose a nursing gastric lavage machine for preventing reflux of comatose patients with gastric tube lavage. The chamber of the buffer tank is in pressure communication with the dirty liquid tank through the communication pipe, and the inside is provided with a piston plate pushed by a spring; the buffer tank is provided with a first flow channel and a second flow channel in communication with the gastric lavage liquid tank respectively; the gastric tube assembly is provided with corresponding first and second lavage channels and suction channels; the adjusting mechanism is linked with the piston plate, when the suction circuit is blocked and the negative pressure of the dirty liquid tank is abnormally increased, the piston plate is pressed to move to the trigger position, driving the adjusting mechanism to execute three actions simultaneously: cutting off the first flow channel, turning on the second flow channel, and blocking the suction circuit; so as to automatically flush the lavage liquid to the blockage of the pipe head, and prevent reflux during treatment; the present application realizes automatic detection, rapid dredging and system reset of blockage, and significantly improves the safety, continuity and nursing efficiency of gastric lavage operation of comatose patients.

The invention belongs to the technical field of medicines, and particularly relates to application of vitamin B6 in preparation of a medicine for treating allergic rhinitis. Animal model experiments of allergic rhinitis prove that the vitamin B6 can significantly reduce the proportion of eosinophilic granulocytes in alveolar lavage fluid of model mice and effectively reduce typical allergic behaviors. Preliminary clinical research also shows that the medicine can significantly improve rhinitis symptoms and life quality of patients, and proves that the medicine has an exact treatment effect.

The invention discloses a heatable constant-temperature delivery device for peritoneal irrigation fluid, which comprises a heating box, an upper cover is clamped at the top end of the heating box, a delivery part is arranged in the heating box, an exhaust part is arranged on the delivery part, and a partition plate is fixedly connected in the heating box; through the separated design of multiple sets of storage cavities in the conveying part, multiple sets of flushing fluid bags can be contained at the same time, parallel heating and independent extraction are achieved, the flushing efficiency in the operation is greatly improved, and the synchronous operation requirements of multi-part abdominal cavity operations are met; and in cooperation with linkage control of the communicating hole and the partition plate, independent extraction of a single liquid bag can be accurately achieved, the interference of liquid mixing of multiple sets of liquid bags is thoroughly avoided, and orderliness of flushing liquid conveying is guaranteed.

The invention relates to the technical field of biological information detection, in particular to a metagenome sequencing diagnosis method and system for Aspergillus pulmonary infection, and the method comprises the following steps: acquiring lavage fluid lysis monitoring data, adjusting shear power output, analyzing fluorescence accumulation trend, adjusting temperature control frequency and substrate interval, identifying mutation points and constructing indexes, screening entropy inversion fragments, and obtaining the metagenome sequencing diagnosis result of Aspergillus pulmonary infection. According to the method, through dynamic matching of the lysate flow rate and the shearing power, the nucleic acid release balance is improved, the temperature control frequency and the substrate interval are adjusted according to the fluorescence signal accumulation trend in the amplification stage, the reaction equilibrium state is established, and the amplification deviation is corrected; and identifying a mutation boundary and reconstructing an index hierarchy according to basic group joint distribution and feature vector change, judging and screening feature sections in combination with information entropy inversion, establishing a probability convergence structure based on coverage depth and repetition frequency, and realizing stable identification of pathogen features and convergence output of classification confidence.

This invention discloses the application of PGLYRP1 in the diagnosis and treatment of sepsis, belonging to the technical field of sepsis treatment and prognosis. The application of PGLYRP1 inhibitors in the preparation of drugs that enhance the body's bacterial clearance capacity and reduce apoptosis in multiple organs is characterized by the inhibitor exerting its therapeutic effect by reducing bacterial load in blood, peritoneal lavage fluid, and spleen; the PGLYRP1 inhibitor is used to antagonize the bacterial clearance inhibition effect mediated by elevated PGLYRP1 levels. This invention reveals that the expression of the novel immunomodulatory factor PGLYRP1 is significantly increased in sepsis patients, and its expression level is significantly positively correlated with poor prognosis of sepsis. Using a CLP-induced sepsis mouse model, this invention found that the expression level of PGLYRP1 in the blood and peritoneal lavage fluid (PLF) of sepsis mice was significantly increased. Treatment with recombinant protein PGLYRP1 significantly increased bacterial load and exacerbated multi-organ damage in septic mice, thereby significantly reducing their survival rate. Conversely, treatment with PGLYRP1 neutralizing antibody significantly reduced bacterial load and alleviated multi-organ damage in septic mice, thereby significantly improving their survival rate.

The present invention provides methods for using pre-operative bodily fluid as a source for a tumor reference genome. In some embodiments, the pre-operative bodily fluid is sequenced to identify a genomic variant specific to a tumor. In some embodiments, assays are performed to detect the presence of the genomic variant specific to a tumor identified in the pre-operative bodily fluid in surgical drain fluid. In some embodiments, the bodily fluids may be blood, urine, serum, plasma, saliva, sweat, milk, mucous, semen, vaginal or urethral secretions, cerebrospinal fluid, or surgical lavage fluid.

The invention provides application of a composition in treatment of knee osteoarthritis. According to the invention, two rat knee osteoarthritis models (the former is called as an ACLT-induced KOA model for short, and the latter is called as a papain-induced KOA model for short) are established by respectively utilizing methods of combining anterior cruciate ligament transection (ACLT) with running and injecting papain composite L-cysteine into an articular cavity; the composition provided by the invention is used for treating two rat KOA models. Results show that the composition can improve knee joint swelling of two KOA rats, increase the pain threshold, improve the abnormal gait state, relieve cartilage injury, inhibit ACLT-induced abnormal change of a KOA rat knee joint subchondral bone microstructure and papain-induced PGE2 content in KOA rat synovial tissue, and can be used for preparing a medicine for treating osteoporosis. The level of inflammatory factors in serum and joint lavage fluid of the two KOA rats is reduced, and the discovery shows that the KOA has important application in treatment of knee osteoarthritis.

The invention relates to the technical field of gastric lavage machines, and discloses an anti-reflux nursing gastric lavage machine suitable for gastric tube lavage of coma patients. A cavity of a buffer tank is in pressure communication with a dirty liquid tank through a communicating pipe, and a piston plate pushed by a spring is arranged in the buffer tank; a first flow channel and a second flow channel which are respectively communicated with the gastric lavage liquid tank are arranged in the buffer tank; a first lavage channel, a second lavage channel and a suction channel which correspond to each other are arranged in the stomach tube assembly; the adjusting mechanism is linked with the piston plate, when the negative pressure of the dirty liquid tank is abnormally increased due to blockage of the suction loop, the piston plate is pressed to move to a triggering position, and the adjusting mechanism is driven to synchronously execute three actions: cutting off the first flow channel, conducting the second flow channel and blocking the suction loop; therefore, the irrigation solution automatically washes the blocked part of the pipe head, and backflow is prevented in the treatment period; according to the invention, automatic detection, rapid dredging and system resetting of blockage are realized, and the safety, continuity and nursing efficiency of gastric lavage operation of coma patients are remarkably improved.

The utility model discloses a kind of experimental mouse alveolus lavage devices, including the fixing platform for fixing mouse, the syringe for providing lavage liquid, the lavage needle for implementing lavage, the lavage needle fixing joint detachably connected with lavage needle, and the hose of connecting syringe and lavage needle fixing joint;Hose is equipped with hose fixing component, hose fixing component has two mutually parallel fixed surfaces, hose hole is opened on hose fixing component, for hose passes through, the outer wall of hose is fixedly connected with the inner wall of hose hole;Fixing platform is equipped with hose fixing mechanism, and hose fixing mechanism includes fixed frame and binding bolt with the thread connection of fixed frame, two fixed surfaces are respectively matched with the inner wall of fixed frame and the end of binding bolt.The utility model is fixed through the hose fixing component arranged on hose and the hose fixing mechanism arranged on fixing platform in the process of lavage, the fixing of hose and lavage needle, it is convenient for user operation.

The present disclosure relates to a portable system for lavaging the intestinal tract of a user. The portable system includes a container that can contain an amount of lavage liquid. The container has a flexible neck having a first interior surface portion and a first exterior surface portion, where the first interior surface portion defines a container opening along a height of the flexible neck of the container. The portable system includes a closure adapted to close the system. The container and the closure can be separated from each other, and the container and the closure form two separate elements of the portable system, which facilitates easy and thorough cleaning of components of the system. The closure of the portable system is further adapted to seal and close the container during use of the system.

The invention belongs to the technical field of medical instruments, and discloses a multi-modal intraoperative bleeding amount real-time estimation method and system, the volume proportion of blood in a mixed solution is calculated through multi-band absorbance information, and the interference of irrigation solution dilution on blood loss amount evaluation is effectively solved; the blood amount in the suction bottle is calculated by combining the weight of the mixed liquid and the suction flow, so that the accuracy of liquid amount measurement is improved; the gauze is tracked and recognized through the surgical field video, the blood content is estimated, and automatic evaluation of the blood content of the gauze is achieved; finally, the Kalman filtering algorithm is used for fusing multi-source data, the accumulated blood loss amount and blood loss rate are dynamically calculated, the real-time performance and robustness of estimation are remarkably improved, and second-level blood loss rate information can be provided. In addition, according to the method, graded warning can be carried out according to the blood loss condition, a blood transfusion pump and an anaesthesia machine are controlled in a linkage mode, and auditable logs are recorded; therefore, the accuracy, the real-time performance, the intelligent level and the traceability of intraoperative blood loss volume evaluation are comprehensively improved.

The invention belongs to the technical field of fungus detection, and particularly relates to a klebsiella pneumoniae detection method based on klebsiella pneumoniae broad-spectrum bacteriophage tail silk protein and a biofilm interference technology (BLI). Primers are designed according to a Klebsiella pneumoniae bacteriophage tail silk protein coding gene, bacteriophage tail silk protein is expressed, after biotinylation, the bacteriophage tail silk protein is combined with a BLI sensor coated with streptavidin to establish a novel high-sensitivity detection probe, and the novel high-sensitivity detection probe is applied to the field of Klebsiella pneumoniae detection. A klebsiella pneumoniae detection method based on klebsiella pneumoniae broad-spectrum bacteriophage tail silk protein and a biofilm interference technology (BLI) is established. The kit can be used for detecting Klebsiella pneumoniae in various clinical samples, including blood, alveolar lavage fluid, urine and other samples, and has good sensitivity and specificity, and the detection limit can reach 100 CFU / mL.

The present invention provides methods for using pre-operative bodily fluid as a source for a tumor reference genome. In some embodiments, the pre-operative bodily fluid is sequenced to identify a genomic variant specific to a tumor. In some embodiments, assays are performed to detect the presence of the genomic variant specific to a tumor identified in the pre-operative bodily fluid in surgical drain fluid. In some embodiments, the bodily fluids may be blood, urine, serum, plasma, saliva, sweat, milk, mucous, semen, vaginal or urethral secretions, cerebrospinal fluid, or surgical lavage fluid.

The present application relates to the technical field of nucleic acid detection, and discloses a general primer pair for detecting the genus of Segniliparus and application thereof. The detection primer pair and identification method provided by the present application are applicable to all species of the genus of Segniliparus, including but not limited to Segniliparus rugosus, and especially unnamed ANI species. The amplification method provided by the present application has low requirements for templates, and is applicable to clinical original samples such as sputum, alveolar lavage fluid, pleural and peritoneal fluid, culture, and environmental original samples such as water, soil and air, and extracted nucleic acid, and does not depend on culture. The method is fast and low-cost, and has low requirements for equipment and detection personnel, and is applicable to laboratories at all levels. The detection reagent based on the present application can be widely applied to related fields such as environmental monitoring, clinical precise detection and epidemiological research.