72 results about "Lavage fluid" patented technology

The invention relates to the technical field of pathogen detection, in particular to a primer probe combination and a kit for detecting various invasive fungi and application of the primer probe combination and the kit. According to the primer and probe combination provided by the invention, a digital PCR (Polymerase Chain Reaction) platform technology is utilized, so that the rapid, efficient, accurate, sensitive and high-specificity simultaneous quantitative detection of Aspergillus fumigatus, Aspergillus, Mucoriums, Cryptococcus and Pneumocystis jirovecii is realized. The rapid detection of the common invasive fungi in sputum, alveolar lavage fluid, urine, blood and other samples is realized, the detection process does not need culture, the detection period is short, the manpower is greatly reduced, and the detection efficiency is improved.

The invention relates to a bronchoalveolar lavage fluid rapid detection method based on a fluorescent staining technology and a corresponding detection device, and aims to improve the detection efficiency of alveolar lavage and sampling screening. The detection device comprises an endoscope assembly, a lavage fluid supply module, a recovery module and a control module. Wherein the recovery module is internally provided with an assembly used for adding a fluorescent coloring agent into the recovery liquid, the fluorescent coloring agent is injected into the recovery liquid in the recovery process of the lavage fluid according to needs, and an electromagnetic drive stirring mechanism is combined, so that dynamic and uniform mixing of the dyeing liquid and the lavage fluid is achieved. The control module automatically adjusts the injection amount, the mixing speed and the stirring duration of the coloring agent according to preset parameters or real-time detection data, and the dyeing reaction time in the operation process is remarkably saved.

The invention discloses a traumatic patient body cavity rewarming method and device based on double-orifice precise temperature control and a storage medium. According to the method, temperature control lavage fluid is synchronously conveyed through double cavities, body temperature and pressure data are collected in real time, parameters are dynamically adjusted, and efficient rewarming is achieved. The device comprises a main machine, a double-cavity rewarming catheter and detachable consumables, the double-cavity catheter comprises a bladder lavage cavity and a stomach tube rewarming cavity and is connected with the main machine through a spiral connector, and the heat exchange efficiency is improved through an internal spiral heat conduction structure; the host integrates an intelligent temperature control system and a double-pump driving and pressure monitoring module, and supports a touch screen operation and wound first-aid mode. The system may store and synchronize data to an external medical system. The scheme solves the problems of low rewarming efficiency, temperature control difference, complex operation and the like in the prior art, and is suitable for low-body-temperature treatment of traumatic patients.

The invention discloses an application of isothunberine or a pharmaceutically acceptable salt thereof in preparation of a medicine for preventing and / or treating asthma diseases. It is found that in an OVA-induced mouse asthma model, the isothunberine significantly reduces airway resistance excited by acetylcholine, improves inflammatory cell infiltration and airway mucus high secretion of asthma model mouse lung tissue, reduces the levels of lgE and OVA-lgE in serum and related inflammatory factors in alveolar lavage fluid, and has the effect of preventing and treating asthma. Therefore, the effect of treating asthma is achieved. Therefore, the isothunberine has the potential of preventing or treating the asthma, provides a new treatment mode for the treatment of the asthma, and has a wide application prospect.

The invention discloses a pulmonary alveolar lavage device for children pneumonia patients, and relates to the technical field of medical instruments, the pulmonary alveolar lavage device comprises a lavage device and a suction pipe, the suction pipe is fixedly communicated with the lavage device, one end of the suction pipe far away from the lavage device is provided with a sealing assembly, the sealing assembly comprises a bearing box, and the bearing box is fixedly communicated with one end of the suction pipe far away from the lavage device. A long rod support is fixedly connected to the outer side wall of the bearing box. By means of continuous operation of the progressive assembly, follow-up full-filled collection bottles and pushing of the previous collection bottle, the full-filled collection bottles are arranged on the workbench in a linear array mode, the filling sequence of the collection bottles can be determined, follow-up detection and analysis are helped, and the accuracy and traceability of the detection result are ensured, for example, the detection efficiency is improved. When the lavage fluid is subjected to microbiological culture and analysis, the dynamic change of an illness state can be judged by knowing the perfusion sequence of the sample, and the change condition of pathogens in the lavage fluid at different stages can be observed.

The invention relates to application of a traditional Chinese medicine composition in preparation of a medicine for treating pneumonia, which is characterized in that the traditional Chinese medicine composition is prepared from gypsum, rehmannia, ophiopogon root, rhizoma anemarrhenae and achyranthes bidentata. The composition disclosed by the invention can be used for remarkably reducing the content of inflammatory factors of IL-6, IL-1beta and TNF-alpha in alveolar lavage fluid of a model rat with pneumonia caused by lipopolysaccharide, reducing the number of white blood cells and neutrophils of rat blood and increasing the percentage of lymphocytes and platelets, so that the traditional Chinese medicine composition has a relatively good treatment effect on pneumonia.

The invention belongs to the technical field of biological medicine, and particularly discloses application of CCL28 as a biomarker and a therapeutic target in sepsis. The invention particularly relates to application of a CCL28 gene or a detection reagent for the expression quantity of the CCL28 gene in preparation of a kit for diagnosis or prognosis monitoring of sepsis. The expression of the novel immunomodulatory factor CCL28 in a sepsis patient is obviously improved, and the expression level of the novel immunomodulatory factor CCL28 is in obvious negative correlation with poor prognosis of sepsis. Through CLP induction of a sepsis mouse model, the invention finds that the expression level of CCL28 in sepsis mouse blood, abdominal cavity lavage fluid (PLF), liver and kidney is significantly increased. By adopting the recombinant CCL28 for treatment, the bacterial load in the mice with sepsis can be remarkably reduced, and the multi-organ injury of the mice with sepsis can be reduced, so that the survival rate of the mice with sepsis can be remarkably improved. In conclusion, the invention explores a novel immunomodulatory protein CCL28, and the novel immunomodulatory protein CCL28 not only can be used as a diagnosis and prognosis biomarker of sepsis, but also can be used as a novel target spot for treating sepsis.

The invention discloses a broncholung lavage fluid composition for treating non-small cell lung cancer, and relates to the technical field of cancer treatment. The preparation method of the bronchus lung lavage fluid composition for treating the non-small cell lung cancer comprises the following steps: S1, performing metagenome sequencing on a BALF sample, S2, grouping by R / NR based on differential strains obtained in S1 to obtain core bacteria, performing single-strain Kaplan-Meier survival analysis on the strains, and determining the survival rate of the single-strain Kaplan-Meier to obtain the bronchus lung lavage fluid composition for treating the non-small cell lung cancer. Finally, seven key microorganisms, namely bronchopulmonary lavage fluid combinations, which are remarkably related to immunotherapy PFS are locked; s3, determining the relationship between the seven key BALF bacteria and the existing immune therapeutic effect marker; and S4, verifying the generation conditions of IL6, IL8 and RANTES cytokines promoted by the seven strains through an in-vitro cell experiment, and revealing the activation and enhancement effects of the seven strains on immune cell functions. According to the invention, the immunotherapy effect of the non-small cell lung cancer patient can be promoted to be improved by combining a plurality of beneficial specific microorganisms in an airway administration mode. In conclusion, the method has a good application prospect.

The present invention relates to a primer-probe combination for early detection of endometrial cancer, a methylation detection kit, and the use thereof. Provided in the present invention is a methylation gene marker for endometrial cancer detection, which specifically comprises at least one of VSX1, SYT1, ZNF132 and VWC2 genes. Measuring the methylation levels of the genes can remarkably distinguish subjects with endometrial cancer from subjects without cancer. The gene marker of the present invention can well identify subjects with endometrial cancer and subjects without cancer from each of tissue / urine / brush smear / swab / blood / lavage fluid specimens, and can achieve a good detection effect simply by means of a single index and further improve the detection efficiency by means of multi-index combination.

The utility model relates to the technical field of drainage tubes, and discloses a sandwich drainage tube which comprises an outer tube and an inner tube, one end of the inner tube is located on the inner side of the outer tube, the other end of the inner tube penetrates through the side wall of the other end of the outer tube and is fixedly connected with the outer tube, and a plurality of lavage channels are communicated between the end, located in the outer tube, of the inner tube and the outer tube. A plurality of leading-out holes and a plurality of leading-in holes are formed in the outer wall of the end, close to the lavage channels, of the outer tube in a penetrating mode, the leading-in holes are communicated with the lavage channels respectively, and the leading-out holes are located between every two adjacent lavage channels respectively. According to the sandwich drainage tube, the inner cavity of the inner tube is isolated from the inner cavity of the outer tube through the lavage channel, so that liquid for lavage into the body of a patient through the inner tube directly enters the body of the patient through the guide-in hole, the lavage liquid is prevented from being directly guided out by the outer tube, the lavage efficiency is improved, and the lavage treatment burden of the patient is relieved.

The invention belongs to the technical field of virus detection, and particularly relates to a double-channel real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection primer, a probe, a detection kit, a detection method and application thereof. The detection sensitivity is high, and the lowest detection limit can reach 10 copies / mL; the detection time period is short, nucleic acid detection can be completed within 45 minutes, and the kit is suitable for clinical and bedside rapid detection and diagnosis; the detection specificity is high, and cross amplification with other 80 pathogens is avoided; the detection accuracy is 100%; virus quantitative analysis can be carried out, and the quantitative linear range is good; the detection repeatability is good, and the precision is high; uDG enzyme is added into a detection system, so that pollution can be prevented; a reference gene is also added into the detection system, so that the quality of the whole extraction and amplification process of the sample can be monitored according to the detection result of the reference gene, and the existence of the RNA of the Enduma virus in samples such as whole blood, serum, plasma, cerebrospinal fluid, urine, alveolar lavage fluid, throat swab and excrement can be detected.

The invention belongs to the technical field of biomedicine, and discloses an antibody for evaluating the curative effect of infantile pneumonia asthma and application of the antibody. The heavy chain CDR sequence of the monoclonal antibody (secreted by the hybridoma cell strain 2B5) is SEQ ID NO: 2-4, the light chain CDR sequence of the monoclonal antibody (secreted by the hybridoma cell strain 2B5) is SEQ ID NO: 6-8, the dissociation constant of the monoclonal antibody and an IL-5R antigen reaches 2.435 * 10 <-1 > / L, and the affinity is high. The antibody can accurately evaluate the curative effect of infantile pneumonia and asthma by detecting the lung function, the ratio of eosinophilic granulocytes in bronchoalveolar lavage fluid and lung tissue inflammation, can also significantly improve airway obstruction and inflammation of asthma model mice, has an effect superior to that of dexamethasone, solves the problems that existing curative effect evaluation is lack of specific tools and has large side effects of therapeutic drugs, and has good clinical application prospects. And a new scheme is provided for curative effect evaluation and adjuvant therapy of infantile pneumonia asthma.

The invention discloses application of irisin in treatment of acute lung injury / acute respiratory distress syndrome. The research finds that the plasma irisin level is obviously reduced in ARDS patients, and the survival prognosis of the patients with the lower irisin level is poorer. Neutrophil as the most inflammatory cells is a cause for promoting lung tissue injury. Further analysis finds that the level of irisin is in negative correlation with the number of circulating neutrophils, and irisin incubation can significantly reduce in-vitro survival promoting (apoptosis delay) phenotypes of neutrophils in ARDS mouse alveolar lavage fluid. Iris receptor integrin alphaVbeta5 inhibitor treatment can promote neutrophil infiltration and aggravate an ARDS process. The invention provides a novel therapeutic drug and strategy for the treatment of acute lung injury / acute respiratory distress syndrome.

The invention relates to the technical field of medical lavage equipment, and discloses a lung lavage flushing and sucking machine which comprises an adjustable water inlet pump for providing lavage fluid input power; one end of the water inlet pipe is connected with the adjustable water inlet pump, and the other end of the water inlet pipe is inserted into the lung through a trachea cannula and is provided with a water injection nozzle; one end of the water outlet pipe is close to the lung to collect waste liquid after lavage, and the other end extends to the far end; the wastewater discharge structure is connected with one end, extending to the far end, of the water outlet pipe and used for receiving wastewater after irrigation. The lavage force is improved through the adjustable water inlet pump, efficient lavage of the deep part in the lung is achieved, meanwhile, continuous collection of waste water is achieved through cooperation of the water outlet pipe and the waste water collecting device, and the lavage efficiency in the lung is improved.

The invention provides a spanishneedles herb exosome as well as a preparation method and application thereof in acute lung injury, and belongs to the technical field of biological medicines. According to the preparation method of the spanishneedles herb exosome provided by the invention, the spanishneedles herb exosome can be extracted from a plant source through a simple method. In one embodiment of the invention, a lung injury model constructed by using LPS is used as an experimental animal to verify the therapeutic effect of the spanishneedles herb exosome. Results show that after the spanishneedles herb exosome is used for treatment, mouse lung tissue injury and inflammatory cell exudation can be relieved, and lung injury caused by LPS can be relieved; tNF-alpha and IL-1beta in lung lavage fluid of a mouse are remarkably reduced, and lung inflammation infiltration of the mouse can be relieved. Therefore, the spanishneedles herb exosome has the effect of treating the acute lung injury and can be used for preparing the medicine for treating the acute lung injury.

This invention belongs to the field of biodetection technology and discloses specific PCR primers and identification methods for the subspecies of Mycobacterium abscessus. The differentiation effect of this invention on the three subspecies of Mycobacterium abscessus is equivalent to whole-genome sequencing, while the established detection method does not rely on sequencing, is rapid and low-cost, and has low requirements for equipment and personnel, making it applicable to laboratories at all levels. The amplification method has low template requirements; it is applicable to raw samples such as sputum, bronchoalveolar lavage fluid, pleural and peritoneal fluid, cultures, and extracted nucleic acids, and is not culture-dependent. Identification of the three subspecies is based on product length rather than amplification negative / positive (presence or absence of bands), avoiding false negative results. The product length differences are significant (above 1000 bp), making results easy to interpret, reducing the impact of electrophoresis errors on results, and improving the accuracy and stability of detection. The detection reagents based on this invention can be widely used in clinical precision testing and epidemiological monitoring, among other related fields.

The invention relates to the technical field of biomedicine, in particular to a group of key biomarkers for predicting endometrial receptivity, including specific microbial strains and metabolites, and a prediction model containing the markers. The method comprises the following steps: collecting a uterine cavity lavage fluid sample, carrying out 16S rRNA gene sequencing and non-targeted metabonomics analysis, and screening out eight key microbial strains and eight key metabolites; based on the markers, the following diagnostic models are constructed: a model only based on eight microbial strains; a model based on only eight metabolites; a combined microorganism and metabolite model; and a clinical data model is further combined. According to the method, a microbiome and metabolome multi-omics conjoint analysis strategy is adopted, strict screening is carried out in combination with LASSO regression, SVM-RFE and other machine learning algorithms, and the obtained biomarker is high in specificity. The constructed model, especially a combined model, shows high prediction precision and good calibration degree through verification of various curves.

This invention discloses the application of a peptide SRD in the preparation of drugs for treating acute lung injury, belonging to the field of biomedical technology. In vivo experiments verified the protective effect of peptide SRD on acute lung injury (ALI), particularly its effect on improving AT2 mitochondrial function. In animal models of ALI, tail vein injection of peptide SRD significantly reduced the protein concentration and total cell count in bronchoalveolar lavage fluid, significantly alleviated the severity of lung injury, lowered histological scores, significantly decreased SOD2 acetylation levels while significantly increasing total SOD2 protein levels, and significantly increased SPC levels (an indicator of AT2 survival). ELISA analysis showed a significant decrease in the levels of IL-6, IL-1β, and TNF-α, indicators of pneumonia in mice. These experimental results confirm that the antioxidant peptide SRD can improve oxidative stress damage in ALI, effectively reduce AT2 cell damage under ALI conditions, promote AT2 regeneration and repair, effectively improve lung function, and reduce the inflammatory response of damaged lungs, thus exhibiting a good therapeutic effect on ALI.

The utility model provides a catheter device for subsection bronchial lavage, which comprises an inner catheter for lavage fluid injection and suction and a mesh basket mechanism for plugging and positioning, and the mesh basket mechanism is arranged on the outer side of the front end of the inner catheter; the mesh basket mechanism comprises a mesh basket body located on the inner side and a film sleeve located on the outer side, and the mesh basket body and the film sleeve are arranged in a stacked mode in the radial direction of the inner catheter. The mesh basket body is formed by weaving a plurality of filaments, and the filaments at least comprise shape memory alloy wires. The utility model can realize the positioning and plugging effect at the specified position in the subsection bronchial tube, the mesh basket mechanism automatically opens to achieve the plugging function, and the utility model has the advantages of convenient operation and strong practicability.

The application discloses a kind of nanoparticles for removing lung mucus and its preparation method and application, it is related to the field of biological medicine.The nanoparticles of the application are composed of inner core and shell coated on the surface of the inner core, wherein the inner core contains citric acid and photoluminescent polymer-aliphatic polyester copolymer;The shell contains deoxyribonuclease and hydrophilic polymer.The nanoparticles provided by the application have excellent biocompatibility and multifunctional integration characteristics, which realizes effective penetration of lung mucus barrier, specific degradation of DNA network, down-regulation of pro-inflammatory factors and inhibition of mucus production in sequence through cascade response mechanism, breaking through the limitations of traditional single therapy.In addition, the in vivo experimental results show that it can significantly reduce the thickness of lung mucus and the protein concentration of lavage fluid, and effectively relieve airway obstruction, and can be used for removing lung mucus.

The present application provides a kind of respiratory tract sampler for collecting avian tracheal lavage fluid, belong to the technical field of poultry respiratory tract sampling instrument, including support part, flexible catheter part, positioning and fixing assembly, elastic catheter head assembly, elastic sliding sleeve assembly and inner integrated tube assembly;Support part opens poultry beak, flexible catheter part extends into trachea, insertion cylinder is recycled in head connection pipe, elastic fin supports flexible fin, avoids bending and reduces mucosa irritation;After reaching the predetermined depth, inner integrated tube assembly provides positive pressure gas, drives insertion cylinder to extend, makes limit inclined table and clamping inclined table self-locking, through-hole is exposed in trachea, positive pressure simultaneously provides oxygen for a short time;Subsequently switch to lavage mode, lavage fluid is washed trachea through through-hole, then switch negative pressure back suction to collect sample;After use, move sliding sleeve, manually push and press flexible head to make insertion cylinder reset, it is convenient to disinfect and reuse;Can reduce poultry discomfort, accurately control lavage position, realize oxygen supply, lavage, back suction multi-functional switching.

The present application relates to the technical field of medical apparatus and instruments, in particular to a bronchoscope alveolar lavage fluid collector, which is applied to a bronchoscope alveolar lavage fluid suction system, the suction system comprises a bronchoscope unit, a waste liquid collecting unit and a negative pressure aspirator, and the bronchoscope unit, the collector, the waste liquid collecting unit and the negative pressure aspirator are connected in series, the collector comprises a valve mechanism, an adapter mechanism and a specimen jar; the specimen jar is detachably installed on the adapter mechanism. The valve mechanism is communicated with the bronchoscope unit and the waste liquid collecting unit respectively, and the adapter mechanism and the detachable assembly structure of the specimen jar are matched, when sampling, the specimen jar is only needed to be installed on the side of the valve mechanism by using the adapter mechanism, and a complete sampling channel can be constructed, after sampling, the sampling channel can be switched to a waste liquid collecting channel by only removing the adapter mechanism, and the pipeline between the bronchoscope unit and the waste liquid collecting unit does not need to be repeatedly detached and connected, so that the pipeline pollution risk and the operation complexity are reduced.

The invention provides a dry powder inhalation preparation taking enoxone as an active ingredient. The preparation is small in particle size, high in lung delivery efficiency, small in dosage and capable of remarkably improving the medication compliance of patients. The dry powder inhalation preparation can significantly reduce the level of white blood cells in pulmonary alveolar lavage fluid of a bronchial asthma rat model, improve the lung function and the lesion degree of trachea, bronchus and lung, and has an obvious treatment effect on bronchial asthma.

The invention belongs to the technical field of biological medicines, and discloses application of AC1903 in preparation of drugs for treating and preventing neutrophil asthma. The invention provides the application of the TRPC5 inhibitor (especially AC1903 or pharmaceutically acceptable salt thereof) in preparation of drugs for preventing and / or treating asthma (especially neutrophil asthma) for the first time. Experiments prove that by intraperitoneal injection of AC1903, the wheezing symptom of a TDI asthma mouse can be remarkably relieved, the airway hyperreactivity, the airway surrounding inflammation level, the total number of inflammatory cells in the airway and the neutrophil count of the TDI asthma mouse are relieved, and secretion of type-2 cytokines and type-17 cytokines of alveolar lavage fluid of the asthma mouse is inhibited. The traditional Chinese medicine composition has a good treatment effect on asthma and has no obvious toxic or side effect.

Disclosed are an apparatus for collecting mouse bronchoalveolar lavage fluid and a use method. The present invention pertains to the technical field of devices for collecting mouse bronchoalveolar lavage fluid. The apparatus comprises a suction and aspiration mechanism, and the suction and aspiration mechanism is connected to a collection mechanism by means of a conveyance tube. The collection mechanism comprises a collection bottle, and a first through hole and a second through hole are formed in a bottle cap of the collection bottle. An air guide tube passes through the first through hole, and a liquid guide tube passes through the second through hole. A first valve is arranged on the air guide tube, and a second valve is arranged on the liquid guide tube. Both the air guide tube and the liquid guide tube are connected to the conveyance tube by means of a three-way joint. The present invention uses the described apparatus for collecting mouse bronchoalveolar lavage fluid and use method. The structure is simple. The arrangement of the collection bottle eliminates the need for multiple transfers during lavage fluid collection that lead to loss. Moreover, controlling the opening and closing of the first valve, the second valve, and a third valve enables suction and aspiration in one device, so that the overall operation is convenient, which facilitates lavage fluid collection.

The invention discloses a product for detecting common pathogens of invasive fungal diseases, and belongs to the technical field of molecular biological detection. The sequences of the primers are as shown in SEQ ID NO.5-6, 8-9, 11-12, 14-15, 17-18, 20-21, 23-24, 26-27, 29-30, 32-33, 35-36, 38-39 and 41-42, and the sequences of the probes are as shown in SEQ ID NO.4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37 and 40. The kit provided by the invention can qualitatively detect 13 common invasive fungi in human alveolar lavage fluid, has the advantages of high specificity and high sensitivity, can still detect under the condition of low nucleic acid content, and compared with mNGS, the kit has the advantages that the detection cost is low, the overall medical cost can be reduced, the detection period is short, one-time detection can be completed only in 1.5-2 hours, and the detection cost is low. The device is suitable for most common instruments, is easy to operate and can be widely applied to medical institutions at all levels.

The invention discloses a child colon lavage device and relates to the technical field of clinical nursing. The device comprises a liquid storage tank, a liquid inlet transmission box, a fluid driving part, a liquid outlet transmission box and a negative pressure generating part, the liquid inlet transmission box is provided with a liquid inlet cavity, the liquid inlet cavity is provided with a first liquid inlet and a first liquid outlet, the first liquid inlet is communicated with the liquid storage tank through a first pressure difference one-way valve, and the first liquid outlet is communicated with a liquid inlet pipe through a second pressure difference one-way valve; the fluid driving part and the negative pressure generating part are in transmission connection, when the fluid driving part generates extrusion force, the negative pressure generating part synchronously moves to generate adsorption force, the liquid inlet process of lavage liquid and the liquid discharging process of lavage backflow liquid form mechanical linkage, the extrusion force and the flow speed of the inlet liquid are matched with the negative pressure adsorption force and the suction rate of the discharged liquid, and therefore the suction efficiency of the lavage liquid is improved. The amount of lavage fluid injected into the intestinal tract can be matched with the amount of sucked backflow fluid in real time, and the risks of abdominal distension, abdominal pain, intestinal mucosa injury and perforation caused by deposition of a large amount of lavage fluid are avoided.

The utility model discloses a pulmonary alveolar lavage device capable of collecting a washing liquid sample, which comprises a lavage device body, a washing liquid tank, a cleaning pipe, a pump machine and a liquid pumping pipe, a liquid outlet hole is arranged at the bottom end of the pump machine, a mounting cavity is integrally formed at the bottom end of the pump machine at the outer side of the liquid outlet hole, and a placing bin is rotatably mounted in the mounting cavity through a connecting mechanism; through the installation cavity, the placement bin, the sample tubes and the like, the multiple sample tubes can be installed in the placement bin and rotationally installed in the installation cavity through the connecting mechanism, the sample tubes correspond to liquid outlet holes formed in the bottom end of the pump machine in position, samples pumped out by the pump machine can be directly injected into the sample tubes, and after the sample tubes are full, the placement bin is rotated; the sample tube is driven by the placing bin to move to the position below the liquid outlet hole to be continuously collected, so that the purpose of replacing the sample tube without temporarily stopping cleaning is achieved, the situation that the time for a patient to bear discomfort during cleaning is prolonged is avoided, and the problems in the background technology are solved.

The present application relates to the technical field of gastric lavage machine, and disclose a nursing gastric lavage machine for preventing reflux of comatose patients with gastric tube lavage. The chamber of the buffer tank is in pressure communication with the dirty liquid tank through the communication pipe, and the inside is provided with a piston plate pushed by a spring; the buffer tank is provided with a first flow channel and a second flow channel in communication with the gastric lavage liquid tank respectively; the gastric tube assembly is provided with corresponding first and second lavage channels and suction channels; the adjusting mechanism is linked with the piston plate, when the suction circuit is blocked and the negative pressure of the dirty liquid tank is abnormally increased, the piston plate is pressed to move to the trigger position, driving the adjusting mechanism to execute three actions simultaneously: cutting off the first flow channel, turning on the second flow channel, and blocking the suction circuit; so as to automatically flush the lavage liquid to the blockage of the pipe head, and prevent reflux during treatment; the present application realizes automatic detection, rapid dredging and system reset of blockage, and significantly improves the safety, continuity and nursing efficiency of gastric lavage operation of comatose patients.

The invention relates to the technical field of riemerella anatipestifer detection, and provides a biomarker and a detection method for detecting riemerella anatipestifer, and the biomarker is a host mucosal lesion product generated by induction of specific virulence factors of riemerella anatipestifer after duck infection. Comprising a mucoprotein cracking fragment containing a linear epitope GTTPSPVP, a sialylated sugar chain containing a specific sialic acid modification motif, and a Muc2 degradation peptide fragment. The detection method comprises the following steps: collecting a sample, pretreating, detecting corresponding markers for excrement, respiratory tract lavage fluid and mucous membrane scrape samples by adopting ELISA (Enzyme-Linked Immunosorbent Assay), an immunochromatography test strip and a lectin chip respectively, and judging a result according to a set threshold value. The kit is high in specificity, covers the whole infection period, can realize early and on-site rapid detection, and improves the diagnosis reliability.