33 results about "Epidemiological Monitoring" patented technology

The invention belongs to the technical field of biological detection, and in particular relates to a babesia caballi disease detection kit based on a fluorescent quantitative PCR (polymerase chain reaction) scalar quantity positive control, which is used for clinical diagnosis and daily monitoring of equine animal infected babesia caballi disease. By designing detection primers as shown by SEQ ID No.1 and SEQ ID No.2 and a fluorescent probe as shown by SEQ ID No.3, combining absolute quantification analysis of a fluorescent quantitative PCR system, and optimizing a reaction system and cycling conditions, a fluorescent PCR detection method for the babesia caballi disease is established. Moreover, the fluorescent PCR detection kit for the babesia caballi disease based on a fluorescent quantitative PCR scalar quantity is established, and is suitable for clinical diagnosis and epidemiological monitoring of the babesia caballi disease.

The invention relates to an on-site rapid high-sensitivity detection kit of prawn infectivity muscle necrosis virus and a detection method thereof. The detection kit comprises a sampling tube, a rinsing tube filled with distilled water, a nucleate degeneration tube filled with a TE buffer solution, an amplification detection tube filled with amplification reaction liquid and nucleic acid dye, a negative control tube, a positive control tube, an FTA membrane, rapid drying liquid, and the like. Compared with the RT-PCR detection method, the detection method in the invention has the advantages of higher specificity, sensitivity and convenience, low cost, convenient use, more accurate and rapid detection and extremely sensitivity, is safe to both human and the environment and can substitute the traditional relevant detection method. The invention can be used in both the indoor laboratory and outdoor production field, has great significance for strengthening the epidemiological monitoring of prawn infectivity muscle necrosis virus, prawn cultivation waterbody monitoring and disease prevention and control and has great promotion and application value.

The invention provides an epidemiological prediction method, which comprises the steps of acquiring time sequence data of epidemiological monitoring; calculating the probability of time point corresponding to each epidemiological monitoring data in the time sequence data belonging to a popular season/a non-popular season turning point, and obtaining a set of probability sequences; determining theprobability peak value in the probability sequence, and obtaining a probability peak value sequence; screening the probability peak value in the probability peak value sequence, and obtaining the popular season/non-popular season turning point of the time sequence data according to the screened probability peak value; determining the types of each popular season/non-popular season turning point, wherein the types comprise a rising turning point and a descending turning point; the rising turning point is the starting point of the epidemiological popular season, and the descending turning pointis the ending point of the epidemiological popular season. The invention further provides a computer device and a readable storage medium. According to the invention, efficient and rapid epidemiological prediction can be realized.

The invention provides a specific oligonucleotide sequence for detecting serotypes 1-8 of enterobacter aerogenes. The oligonucleotide contains: (1), a DNA fragment selected from wzy genes of the enterobacter aerogenes of types 1, 2, 3, 5, 6, 7 and 8; (2), a DNA fragment selected from orf8 genes of the enterobacter aerogenes of type 4. A suspension array detection system and method for the serotypes 1-8 of the enterobacter aerogenes are established through combination with a Bio-Plex 200 suspension array system of the Bio-Rad company, the blank of serological typing technology of the enterobacter aerogenes is filled up, and the invention has great significance in clinical detection and epidemiological monitoring of the important pathogenic bacterium.

The invention relates to a preparation method of an indirect ELISA detection kit based on theileria equi EMA1. The preparation method comprises the preparation of theileria equi EMA1 and the establishment of a theileria equi disease rELISA detection method. The preparation method overcomes the defects of high cost, poor specificity and time consuming of a conventional detection kit, and establishes the theileria equi disease rELISA detection method which is suitable for the clinical diagnosis and epidemiological monitoring of theileria equi disease.

The invention discloses a method for preparing a pure protein derivative of tubercle bacillus by using a mycobacterium tuberculosis attenuated strain H37Ra, and belongs to the technical field of biological protein preparation. The mycobacterium tuberculosis attenuated strain CMCC93020 (H37Ra) is used as a strain, and a finished product of the pure protein derivative of the tubercle bacillus is obtained through amplification, inactivation, salting-out precipitation, degerming filtration, inspection, dilution and subpackaging. The defects in the prior art that strains have great harm to operators and the environment and have high requirements for production conditions (P3 production workshops) are overcome, and finally the protein which can be used for clinical diagnosis of tuberculosis, screening of bacillus calmette-guerin vaccine inoculation objects, monitoring of organism immune responses after bacillus calmette-guerin vaccine inoculation and epidemiological monitoring is obtained.

The invention discloses a production method of a pure protein derivative of tuberculin, and belongs to the technical field of biological protein preparation. An attenuated strain of mycobacterium tuberculosis ATCC25177 and/or CMCC93020 is used as a strain; and the finished product of the pure protein derivative of tuberculin is obtained through amplification, inactivation, salting-out sedimentation, degerming filtration, inspection, dilution and sub-packaging. The defect that strains have great harm to operators and the environment and have high requirements for production conditions (P3 production workshops) and the like in the prior art are overcome; and a protein, which can be used for clinical diagnosis of tuberculosis, screening of bacillus calmette-guerin vaccine inoculation objects, monitoring of organism immune response after bacillus calmette-guerin vaccine inoculation and epidemiological monitoring is finally obtained.

The invention discloses colloidal gold test paper for detecting IBV. The colloidal gold test paper comprises a support plate, a coating film, a sample pad, a gold-labeled antibody pad, water absorbingpaper, a detection line and a quality control line, and is characterized in that the gold-labeled antibody pad is labeled with an anti-IBV IgG antibody, the detection line is coated with an anti-IBVN protein monoclonal antibody, and the quality control line is coated with a goat anti-mouse IgG antibody. The invention also discloses an index method of the colloidal gold test paper for detecting IBV. The test paper prepared by the invention is based on the principle of a double-antibody sandwich ELISA antigen detection method, is used for detecting IBV antigens so as to intuitively, quickly, simply and conveniently detect virus infection and achieve good specificity and the purpose of accurate and quick detection, can provide technical support for immune monitoring and epidemiological monitoring research of avian infectious bronchitis, and has important significance in prevention and control of avian diseases.

The invention relates to a field fast high-sensitivity detection kit and a detection method for prawn hepatopancreatic parvovirus. The detection kit comprises a sampling pipe, a rinsing pipe filled with distilled water, a nucleic acid denaturation pipe filled with a TE buffer solution, an amplification detection pipe filled with amplification reactant liquid and nucleic acid dyes, a negative control pipe, a positive control pipe, an FTA diaphragm, quick drying liquid thereof and the like. The detection method has higher specificity, sensitivity and convenience than that of the PCR detection method, has the advantages of low cost, convenient operation, more accurate and faster detection and extremely high sensitivity, is safe to both human beings and the environment, and can replace the existing relevant detection methods such as the pathological section method, the electron microscope method and the PCR detection method. The detection kit and the detection method can be used in the indoor laboratory or the outdoor production field, are of great significance for enhancing the epidemiological monitoring and the disease control on prawn hepatopancreatic parvovirus, and have higher popularization and application values.

The invention discloses a primer group using a vtaA9 gene as a target spot and application of the primer group to identification and diagnosis of haemophilus parasuis. Researches find that a PCR (Polymerase Chain Reaction) method using the vtaA9 gene as the target spot is capable of accurately distinguishing different pathogenicities, and can simultaneously settle in swine upper respiratory tract H.parasuis and similar strains A.indolicus, A.minor and A.porcinus thereof. In addition, based on the vtaA9 gene sequence, the invention designs and synthesizes a specific primer, wherein nucleotide sequences of the primer are shown as SEQ ID NO.1 and SEQ ID NO.2. Specificity experiments indicate that according to the method disclosed by the invention, the H.parasuis and the A.indolicus as well as the A.minor and the A.porcinus can be completely distinguished. Sensitivity experiments indicate that by using the method disclosed by the invention, genomes DNA of not less than 20CFU/ml and 5pg/mu l can be detected. In addition, a positive result of the PCR method using the vtaA9 as the target spot for a clinical sample is higher than that of isolated culture of a strain and a classic 16S rRNA (ribosomal ribonucleic acid) PCR method. The invention provides a novel method for differential diagnosis of haemophilus parasuis and epidemiological monitoring.

The invention discloses a molecular detection method for rapid identification of genotypes of anti-fenoxaprop-P-ethyl Beckmannia syzigachne containing Ile-1781-Leu mutation based on LAMP technology. The detection method can be used for early warning and epidemiological monitoring of Beckmannia syzigachne containing the Ile-1781-Leu mutation. The detection method comprises altogether three main steps: (1) respectively extracting genomic DNA of a sample to be detected; (2) designing specific primers and carrying out LAMP isothermal amplification; and (3) adding the fluorescent dye SYBR Green I into an LAMP amplification product and observing color changes, or carrying out separation through agarose gel electrophoresis with 3.0% agarose and observing amplification results, wherein whether there is a population of Beckmannia syzigachne containing the Ile-1781-Leu mutation is determined based on the results, a population of Beckmannia syzigachne is determined to be a population containing the Ile-1781-Leu mutation if the LAMP amplification product is yellow or electrophoretogram is in the form of a scalariform strip, and a population of Beckmannia syzigachne is determined to be a population not containing the Ile-1781-Leu mutation if the LAMP amplification product is orange or electrophoretogram has no strip amplification. According to the invention, isothermal amplification is realized, expensive instruments and equipment and tedious electrophoresis process are not needed, and detection time and cost are substantially reduced. Compared with common PCR, the detection method provided by the invention has the characteristics of high sensitivity, strong specificity, high detection accuracy, etc.

The invention belongs to the biotechnical field, relating to a method for detecting drug-resistant related gene mutation of campylobacter jejuni macrolide drugs. The method makes use of a realtime fluorescence quantitative PCR TaqMan probe technology to design a specific primer and a probe according to a nucleic acid sequence of a V zone inside a campylobacter jejuni macrolide drug-resistant mutation zone 23S rDNA; moreover, through optimizing a TaqMan realtime fluorescence quantitative PCR reaction system and conditions, related drug-resistant mutational sites of target genes are accurately detected. The method can realize quick, accurate and simultaneous detection of two main drug-resistant mutational sites of campylobacter jejuni macrolide type, and has better specificity and higher sensitivity than a related molecular biology detection method reported at present; moreover, the method has simple operation, high accuracy and quick speed compared with the prior sequencing and drug sensitive experimental method. The method provides an effective technical means for molecular epidemiological monitoring of macrolide campylobacter jejuni resistance.

The invention relates to an on-site rapid high-sensitivity detection kit and a detection method of prawn yellow head virus. The on-site rapid high-sensitivity detection kit comprises a sampling tube, a rinsing tube filled with distilled water, a nucleic acid denaturation tube filled with TE buffer liquid, an amplification detection tube filled with amplification reaction liquid and nucleic acid dye, a negative control tube, a positive control tube, an FTA membrane, quick drying liquid, and the like. Compared with an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection method, the detection method has higher specificity, sensitivity and convenience, and has the advantages of low cost, convenient use, more accurate and quicker detection, high sensitivity, and high safety to human and environment, and can replace the traditional related detection methods, such as an electron-microscopic observation method, an RT-PCR detection method, and the like. The on-site rapid high-sensitivity detection kit and the detection method can be used in both an indoor laboratory and a production site in the field, have great significance for strengthening the prawn yellow head virus epidemiological monitoring, prawn culturing water monitoring and disease control and prevention, and have high popularization and application value.

The invention provides the application of mycobacteria Ku protein. The invention provides a novel marker which belongs to ku gene/protein of an NHEJ system and can be used for the identification of mycobacteria and the differential identification of MTBC and NTM. The prevalence of the ku gene in mycobacteria and the rarity in prokaryotic cells determine the applicability and high specificity of adetection technology based on the marker to the entire mycobacterium. Based on the ku gene/protein, rapid identification methods such as PCR, RT- PCR, LAMP-PCR and enzyme-linked immunosorbent assay (ELISA) and immunocolloidal gold can be established, and corresponding reagents can be developed. A detection reagent based on the invention can be widely applied to related fields such as auxiliary diagnosis of tuberculosis and epidemiological monitoring.

The invention belongs to the technical field of biology, and particularly relates to a mycobacterium tuberculosis antigen protein Rv1808 and an application of an epitope peptide thereof. A detection reagent prepared from the Rv1808 protein and the epitope peptide thereof can be widely applied to the related fields of auxiliary diagnosis of tuberculosis, epidemiological monitoring and the like, and tuberculosis vaccines and antituberculosis drugs prepared from the Rv1808 protein and the epitope peptide thereof can be applied to prevention and treatment of tuberculosis.

The invention relates to the technical field of molecular biology and immunology, and particularly discloses application of a T cell epitope protein Rv1566c-444 of mycobacterium tuberculosis. The invention provides an application of a mycobacterium tuberculosis T cell epitope protein Rv1566c-444 in preparation of a tuberculosis detection reagent and a vaccine. Wherein the amino acid sequence of the mycobacterium tuberculosis T cell epitope protein Rv1566c-444 is shown as SEQ ID NO.4, or is an amino acid sequence which is formed by replacing, deleting or adding one or more amino acids to the sequence and has the same antigenicity and immunogenicity. The mycobacterium tuberculosis T cell epitope protein Rv1566c-444 provided by the invention can reduce false negative caused by T cell stimulation by an intrinsic protein antigen of a kit, so that the detection sensitivity is improved. The cell immune response is good, and the vaccine can be widely applied to the related fields of auxiliary diagnosis of tuberculosis, epidemiological monitoring, infection screening and the like, and provides a new thought for the development of a new anti-tuberculosis vaccine.

The invention provides specific oligonucleotide sequences used for detecting 26 antigenic serotypes of enterobacter cloacae and application thereof. Oligonucleotides comprise (1) two DNA fragments, shown in SEQ ID NO:1-SEQ ID NO:46, respectively selected from wzy genes of enterobacter cloacae O1 type, O3-O22 type, O24 type, O26-O27 type and O30 type and (2) two DNA fragments, shown in SEQ ID NO:47and SEQ ID NO:48, selected from a wzt gene of enterobacter cloacae O23 type. The invention further establishes a multiplex-PCR detection system and method for detecting the 26 antigenic serotypes ofthe enterobacter cloacae by utilizing the oligonucleotides. A serological typing technology for the enterobacter cloacae by utilizing a molecular biological method is established for the first time, which is of great significance for clinical detection and epidemiological monitoring of the important pathogenic bacterium.