34 results about "Epidemiological Monitoring" patented technology

The invention discloses a kit for detecting drug resistance genes of ten trimethoprim drugs. The kit comprises an amplification primer combination for carrying out specific amplification on the drug resistance genes of the ten trimethoprim drugs and a specific probe for detecting on the drug resistance genes of the ten trimethoprim drugs. The invention further discloses a method for detecting thedrug resistance genes of the ten trimethoprim drugs by using the kit. The method for detecting the drug resistance genes of the trimethoprim drugs and the kit thereof have the characteristics of highintegration degree, high sensitivity, good specificity, stable and reliable detection results and low cost, can be widely used for distribution and epidemiological monitoring of the drug resistance genes of the trimethoprim drugs, and provides an effective tool for rational drug use and effective reduction and delay of drug resistance.

The invention relates to a field fast high-sensitivity detection kit and a detection method for prawn hepatopancreatic parvovirus. The detection kit comprises a sampling pipe, a rinsing pipe filled with distilled water, a nucleic acid denaturation pipe filled with a TE buffer solution, an amplification detection pipe filled with amplification reactant liquid and nucleic acid dyes, a negative control pipe, a positive control pipe, an FTA diaphragm, quick drying liquid thereof and the like. The detection method has higher specificity, sensitivity and convenience than that of the PCR detection method, has the advantages of low cost, convenient operation, more accurate and faster detection and extremely high sensitivity, is safe to both human beings and the environment, and can replace the existing relevant detection methods such as the pathological section method, the electron microscope method and the PCR detection method. The detection kit and the detection method can be used in the indoor laboratory or the outdoor production field, are of great significance for enhancing the epidemiological monitoring and the disease control on prawn hepatopancreatic parvovirus, and have higher popularization and application values.

The invention discloses a primer group using a vtaA9 gene as a target spot and application of the primer group to identification and diagnosis of haemophilus parasuis. Researches find that a PCR (Polymerase Chain Reaction) method using the vtaA9 gene as the target spot is capable of accurately distinguishing different pathogenicities, and can simultaneously settle in swine upper respiratory tract H.parasuis and similar strains A.indolicus, A.minor and A.porcinus thereof. In addition, based on the vtaA9 gene sequence, the invention designs and synthesizes a specific primer, wherein nucleotide sequences of the primer are shown as SEQ ID NO.1 and SEQ ID NO.2. Specificity experiments indicate that according to the method disclosed by the invention, the H.parasuis and the A.indolicus as well as the A.minor and the A.porcinus can be completely distinguished. Sensitivity experiments indicate that by using the method disclosed by the invention, genomes DNA of not less than 20CFU / ml and 5pg / mu l can be detected. In addition, a positive result of the PCR method using the vtaA9 as the target spot for a clinical sample is higher than that of isolated culture of a strain and a classic 16S rRNA (ribosomal ribonucleic acid) PCR method. The invention provides a novel method for differential diagnosis of haemophilus parasuis and epidemiological monitoring.

An epidemic prediction method, the method obtains the time series data of epidemic monitoring; calculates the probability that each time point corresponding to the epidemic monitoring data in the time series data belongs to the turning point of the epidemic season / non-epidemic season, and obtains a set of probabilities sequence; determine the probability peak in the probability sequence to obtain the probability peak sequence; screen the probability peak in the probability peak sequence, and obtain the popular season / non-popular season turning point of the time series data according to the screened probability peak ; Determine the type of each epidemic season / non-epidemic season turning point, the type includes a rising turning point and a falling turning point, the rising turning point is the starting point of the epidemic season, and the falling turning point is the ending point of the epidemic season. The invention also provides a computer device and a readable storage medium. The invention can realize efficient and rapid epidemic prediction.

The invention discloses a primer group for quickly detecting the gyrA gene mutation site of campylobacter jejuni and an application thereof. The primer group consists of a specific primer pair and a sequencing primer, wherein the specific primer pair consists of a single-strand DNA molecule A shown by the sequence 1 and a single-strand DNA molecule B shown by the sequence 2, and the sequencing primer is a single-strand DNA molecule C shown by the sequence 3. By adopting the campylobacter jejuni to be detected as a template and adopting the specific primer pair, a gyrA gene segment including a 257 mutation site is obtained through a PCR (polymerase chain reaction), and a DNA single strand can be obtained through single-strand separation and purification of PCR products and is directly put into a pyrosequencing apparatus for sequencing. By adopting the primer group disclosed by the invention, the C257T mutation of campylobacter jejuni can be identified so as to determine whether the campylobacter jejuni to be detected tolerates quinolone antibiotics at a high level. A powerful technical means is provided for the detection of anti-quinolone campylobacter jejuni as well as molecular epidemiological monitoring.

The invention relates to mycobacterium tuberculosis antigen protein Rv0585c and application of a T cell epitope peptide thereof in preparing tuberculosis detection reagents, vaccines and medicines. The epitope peptide is selected from P24, P26 and P31 which have amino acid sequences shown as SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3 respectively. A mycobacterium tuberculosis protein antigen Rv0585c and the T cell epitope peptide thereof serve as irritants and are used for specific T cell and B cell immunoreaction caused by mycobacterium tuberculosis infection; compared with an existing adopted complete antigen, the false positive result caused by antigen impurity can be reduced. The detection reagents prepared from the protein antigen Rv0585c and the epitope peptide of the protein antigen Rv0585c can be widely used for auxiliary diagnosis, epidemiological monitoring and other related fields of tuberculosis. Tuberculosis vaccines and antituberculosis medicines prepared from the protein antigen Rv0585c and the epitope peptide of the protein antigen Rv0585c can be used for preventing and treating tuberculosis.

The invention relates to an on-site rapid high-sensitivity detection kit and a detection method of taura syndrome virus (TSV). The on-site rapid high-sensitivity detection kit comprises a sampling tube, a rinsing tube filled with distilled water, a nucleic acid denaturation tube filled with TE buffer liquid, an amplification detection tube filled with amplification reaction liquid and nucleic acid dye, a negative control tube, a positive control tube, a FTA membrane, quick drying liquid, and the like. The detection method has higher specificity, sensitivity and convenience than the TR-PCR detection method and has the advantages of low cost, no toxicity, high safety, convenient use, more accurate and quicker detection and high sensitivity and can replace the prior related detection methods, such as a pathological section method, an electron-microscopic observation method, an antibody detection method and a TR-PCR detection method. The on-site rapid high-sensitivity detection kit and the detection method can be both used in an indoor laboratory and a production site in the field, have significance for strengthening the TSV epidemiological monitoring and the disease control and prevention and have high popularization and application value.

The invention discloses a primer pair and a primer probe composition used for identifying human adenovirus type 55 and application thereof. A kit for aided identification and / or quantitative determination of human adenovirus type 55 comprises the specific primer pair composed of a single-stranded DNA molecule as shown in a sequence 1 in a sequence table and a single-stranded DNA molecule as shown in a sequence 2 in the sequence table. The kit further comprises a specific probe which has a nucleotide sequence as shown in a sequence 3 in the sequence table or shown in a reverse complementary sequence of the sequence 3 in the sequence table. According to the invention, the disadvantages of a great consumption amount of samples, complex experimental operation, low detection sensitivity, poor specificity and the like of traditional detection technology are overcome, and the kit has high application specificity, shows detection sensitivity as same as that of a 0.008PFU / reaction system and is applicable to high flux screening, especially to scientific research, clinical practice, epidemiological monitoring of viruses, etc.

The invention provides the application of mycobacterial Ku protein. The invention provides a new marker, the ku gene / protein belonging to the NHEJ system, which can be used for the identification of mycobacteria and the differential identification of MTBC and NTM. The ubiquity of ku gene in mycobacteria and the rarity in prokaryotic cells determine the applicability and high specificity of the detection technology based on this marker to the entire genus of mycobacteria. Based on the ku gene / protein, rapid identification methods such as PCR, RT-PCR, LAMP-PCR, enzyme-linked immunoassay (ELISA), and immune colloidal gold can be established and corresponding reagents can be developed. The detection reagent based on the invention can be widely used in related fields such as auxiliary diagnosis of tuberculosis, epidemiological monitoring and the like.