143results about How to "Reduce false positive results" patented technology

Systems and methods are disclosed for monitoring data transmissions on a network and detecting compromised networks. The systems and methods monitor communications involving network hosts and analyze the communications in view of the business function of the hosts. In certain embodiments the analysis is performed by associating a set of rules of operation for the sessions, hosts, and / or environment, and analyzing data packet transmissions to ascertain violations of the rules.

The present invention relates to a system and a method for evaluating the risk of carrying a fetus with genetic anomalies such as aneuploidy and in particular, to such a system and method where a screening system and method is provided to identify fetus' having trisomy-21 (Down's syndrome) with the use of biochemical marker concentrations evaluated from the maternal blood serum.

The invention belongs to the technical field of nucleic acid detection, which concretely relates to a single primer-initiated nucleic acid constant temperature amplification method. The technical problem which need to be solved by the method is to provides the single primer-initiated nucleic acid constant temperature amplification method, the method avoids the disadvantages of complex primer design and high detection cost in a traditional constant temperature detection method in the prior art. The provided technical scheme is a single primer-initiated nucleic acid constant temperature amplification method, which is characterized in that the primers enable self complementation and pairing after pairing with nucleic acid by designing a primer sequence, then a stem-and-loop structure is formed, the primer can be disengaged from the target nucleic acid, and then nucleotide fragments can be continuously generated under combined action of cutting enzyme and polymerase. The technical scheme is completed under isothermal condition, the equipment capable of keeping constant temperature can be used for performing the method, and the operation is convenient; only one primer is required during the amplification process, so that detection scheme is simpler and easier to be carried out, and the nucleic acid amplification and detection efficiency can be increased.

The invention discloses a method for verifying peptide with tandem mass-spectrometric data which comprises steps of: splintering on the target peptide to generate trial tandem mass-spectrometric; theoretically splintering on a plurality of candidate peptides to generate multiple theoretical tandem mass-spectrometric; calculating the similarity of theoretical tandem mass-spectrometric and trial tandem mass-spectrometric with radial basic function which comprises an index; according to the calculated similarity, selecting the proper peptide which theoretical tandem mass-spectrometric is most similar with trial tandem mass-spectrometric as verified results. The inventive method has the advantages of having higher accuracy and lower false positive results.

The invention discloses a primer probe composition and kit for detecting the polymorphism of a human CYP2C19 gene and application. The primer probe composition comprises three pairs of specific primers for amplifying CYP2C19*2, CYP2C19*3 and CYP2C19*17 sites and three specific fluorescent probes. The primers and the probes have high sensitivity, strong specificity and strong anti-interference capability; the polymorphism of the gene is detected by adopting a manner combining asymmetric PCR (Polymerase Chain Reaction) amplification and a fluorescent probe melting curve analysis technology; different gene types can be effectively distinguished according to the quantity of melting peaks and Tm value and result interpretation is convenient, clear and objective. Single-tube sampling can be usedfor detecting 6 mutation types of 3 gene sites at the same time; the operation is simple and convenient and the detection efficiency is improved; a large batch of samples can be detected and clinicaloperation is facilitated.

A process of rapid and highly accurate analysis of spectral data, includes both a linear scanning (LINSCAN) method and an advanced peak detection method for pattern recognition. One or both of the methods are used to support the detection and identification of chemical, biological, radiation, nuclear and explosive materials. The spectra of various targets can be analyzed by the two spectral analysis methods. These two methods can be combined for dual confirmation, greater accuracy, and to reduced false positives and false negatives, relative to what can be accomplished by either alone.

The invention discloses FISH probe groups for detecting a novel coronavirus SARS-CoV-2. The probe groups comprise at least two fluorescent probe groups among a first fluorescent probe group taking anS gene as a target, a second fluorescent probe group taking an E gene as a target, a third fluorescent probe group taking an M gene as a target, a fourth fluorescent probe group taking an ORF 3a geneas a target, a fifth fluorescent probe group taking an N gene as a target, and a sixth fluorescent probe group taking an ORF 1ab gene as a target. In the at least two fluorescence probe groups, fluorescence molecules labeled by at least one fluorescence probe group have a fluorescence emission spectrum different from that of the other fluorescence probe groups. The FISH probe groups have high specificity and sensitivity, can realize positioning detection of SARS-CoV-2 in a to-be-detected sample, obtain the distribution and relative quantification conditions of the SARS-CoV-2, are an effectivesupplement for novel coronavirus nucleic acid detection, and have important clinical application value.

The invention discloses a primer and probe composition for detecting polymorphism of human CYP2C9 and VKORC1 genes, a kit and application. The primer and probe composition comprises three pairs of specific primers for amplifying CYP2C9*2, CYP2C9*3 and VKORC1 sites, and three specific fluorescent probes. The primers and the probes, disclosed by the invention, have high sensitivity, strong specificity and strong anti-interference capability; a manner combining an asymmetric PCR (Polymerase Chain Reaction) amplification and fluorescent probe melting curve analysis technologies is used for detecting the gene polymorphism; different gene types can be effectively distinguished according to the quantity and Tm value of a melting peak; a result is convenient, clear and objective to judge and read.Single-tube sampling can be used for simultaneously detecting 6 mutation types of 3 gene sites; the primer and probe composition is simple and convenient to operate and the detection efficiency is improved; a large batch of samples can be detected and clinical operation is facilitated.

The invention discloses a micro-molecule fluorescent probe of a phenyl furan hERG potassium channel and application thereof. The structure general formula of the fluorescent probe is as shown in a formula (I), wherein R1 is a single substituted group or a polysubstituted group of halogen, alkyl or alkoxy; R2 is fluorophore; n is 1-6; the piperazine ring and the fluorophore are connected by an alkyl chaing containing 1-6 carbons. The molecule of the fluorescent probe can be used for marking an hERG potassium channel, can be used for screening activity of an hERG potassium channel inhibitor and evaluating the cardiotoxicity of commercial medicines, or used as a tool drug for pharmacological, pathological and physiological researches related to hERG potassium channels. Furthermore, the preparation method of the compound is mild in reacting condition, the raw materials have low price and are easily available, and the operation and posttreatment are simple.

The invention relates to a dilute of an alkaline phosphatase maker and application thereof. The diluent comprises a buffer, a protein, a surfactant, a zinc ion, a magnesium ion, a preservative and anadditive, wherein the additive comprises a blocking agent and / or alkaline phosphatase; and the alkaline phosphatase is a low-active alkaline phosphatase and / or inactive alkaline phosphatase. The invention creatively adds the blocking agent and / or alkaline phosphatase to the traditional diluent for diluting alkaline phosphatase markers, and can well reduce non-specific reactions and ultimately reduce the probability of false positive results in clinical samples to improve the accuracy of detection and analysis results.

The invention discloses a method for identifying a glycopeptide segment, which comprises the following steps: filtering a mass spectrogram; acquiring a single-charge mass number; constructing a mass number network; and retrieving a theory library. The invention also discloses a device for identifying the glycopeptide segment, which comprises a mass spectrogram filtering unit, a single-charge massnumber acquisition unit, a mass number network construction unit and a theory library retrieving unit. Compared with the conventional method for identifying the glycopeptide segment, the identification method and the identification device can efficiently acquire more accurate identification results in which false positive results are obviously reduced.

The invention discloses a CPA primer, kit and detection method for escherichia coli O157:H7. The CPA primer designed for a target rfbE comprises stripping primers 4s and 5a, a crossing amplification primer 2a1s and specific primers 2a and 3a; the nucleotide sequences of the primers are shown as SEQ ID NO.1-NO.5 respectively. A crossing priming amplification reaction detection and identification system designed for specific target sequences rfbE and stx1 of E.coli O157:H7 overcomes the defects of long required cycle, low sensitivity, high cost and difficult field application of methods in the prior art. By selecting a conserved region of the specific sequences rfbE and stx1 of a target strain, a pair of stripping primers, the crossing primer and the specific primers are designed to construct the crossing priming amplification reaction system, and a detection result is obtained within about 60 minutes to shorten the traditional cycle for detecting escherichia coli.

An object of the present invention is to provide a labeled particle having a high reactivity with an antigen and a suppressed non-specific adsorption, and an immunochromatographic method using the labeled particle. The present invention provides a labeled particle, wherein a fragmented antibody is immobilized to a labeling substance via a chemical bond.

A method and system for an early warning detection of bioterrorism events includes obtaining temperature readings from a statistical sample of individuals in a community, and comparing the individual readings to one or more detection thresholds spaced apart by predetermined values with at least one of the thresholds being below the normally accepted temperature range defined as a low-grade fever. The comparison is then used to identify and evaluate a community's potential infection by a biological warfare agent so that early therapeutic action may be taken.

The invention discloses a circulation tumor cell separation and enrichment micro-fluidic chip and an enrichment method thereof. The circulation tumor cell separation and enrichment micro-fluidic chip comprises a sample treatment chamber and a separation purification chamber, wherein the separation purification chamber comprises a micro channel, a magnetic field adsorption part, a magnetic collection chamber, a cell collection chamber and a filtering membrane; a sub-channel is formed inside the micro channel; the magnetic field adsorption part is arranged on the inner wall of the sub-channel; one end of the micro channel is communicated with the sample treatment chamber; the other end of the micro channel is communicated with the magnetic collection chamber and the cell collection chamber in sequence; the magnetic collection chamber is used for collecting magnetic beads which are not adsorbed by the inner wall of the sub-channel; the filtering membrane which is capable of retaining circulation tumor cells is arranged inside the cell collection chamber; and a liquid outlet from which a liquid filtered by the filtering membrane flows out is formed in the cell collection chamber. The circulation tumor cell separation and enrichment micro-fluidic chip is efficient, rapid, good in specificity and high in accuracy.

The invention relates to a group of primers and probes and a corresponding kit, including a pair of primers which can simultaneously detect West Nile virus and the reference gene and two TaqMan probes against the West Nile virus and the reference gene, so as to reduce the number of the primers and the probes in a PCR reaction system from the usual 6 to 4, further to realize the high efficient amplification of the West Nile virus and the reference gene in the same tube, thus realizing the detection of the target genes of the different species, having strong universality, reducing the number of the primers and the probes, the cost of the reagents and the operation steps, and reducing the pollution chances which need to be avoided generally in a PCR experiment. The kit of the invention is applicable to the detection of the West Nile virus in the scientific research or the clinical aspect, especially the monitoring of the West Nile virus in the prevention and the control works of viral diseases.

The present invention provides a device and a method for detecting gene sequence, combines detecting system based on nano particle principle and electric field device to control and speed micro matrix detection and provides an autoamtic control process via analysis of the feedback signal of nano particle probes on the chip. The present invention also relates to the relevant gene sequence detecting method. The present invention also provides the autoamtic feedback and automatic regulation device and method for the crossing and de-crossing process of the micro matrix gene chip. The present invention has high detection speed, high sensitivity and low cost compared with fluorescent probe method.

The invention discloses a prawn IHHNV (infectious hypodermal and hematopoietic necrosis virus) nucleic acid isothermal amplification detection kit which comprises a grinding fluid tube containing a grinding fluid, a nucleic acid extracting solution tube A containing a sodium acetate solution, a nucleic acid extracting solution tube B containing absolute ethyl alcohol, a nucleic acid extracting solution tube C containing an alcohol solution with a mass concentration of 70 percent, a TE (tellurium) buffer solution tube containing a TE buffer solution, a UNG (uracil-DNA glycosidase) enzyme tube containing uracil DNA (deoxyribonucleic acid) glycosylase, an LAMP (Loop-mediated isothermal amplification) reaction liquid tube containing an LAMP reaction liquid, a BstDNA polymerase tube containing Bst DNA polymerase, a color-developing agent tube containing nucleic acid dye SYBR Green I, a positive control nucleic acid tube containing prawn IHHNV positive DNA and a negative control tube containing sterilizing double distilled water. The invention also provides a method for detecting the prawn IHHNV by utilizing the detection kit. The method has the characteristics of low cost, high detection sensitivity and easy site operation.

The invention discloses a chryseobacterium gleum of keratinase and a method for separating the chryseobacterium gleum. The Latin name of the chryseobacterium gleum is Chryseobacterium L99; the chryseobacterium gleum is stored in CGMCC with the collecting number of CGMCC No.2295. The invention discloses the morphological properties of the bacterium: the bacterium body has a rod shape, no spores are produced, the bacterium does not have motility and the Gram pigmentation shows negative. The invention discloses whole-cell fatty acid of the bacterium. The main components in weight percent are as follows: 47.59 percent of 13 methyl 14 acid, 15.72 percent of dihydroxy-13-methyl myristic acid and / or Omega-7-cis-13 methyl-15 acid, 13.91 percent of trihydroxy-15-methyl 16 acid, and 9.48 percent of Omega-9-cis-14 methyl hexadecanoic acid. The invention also discloses the whole series of a 16 S rDNA of the bacterium. The separating method comprises the following steps that: the common nutritional medium is bred in enrichment, the keratin is taken as the only SiCN source solid culture medium prescreening operation and is taken as the secondary screening of the only SiCN source solid culture medium. The method is effective and rapid and the strain has strong ability to produce enzyme.

The invention relates to the technical field of virus detection, in particular to a PCR primer group and kit for detecting African swine fever viruses based on double genes and application. The primergroup and method are high in detection specificity, good in repeatability and high in sensitivity, and meanwhile have the advantages of being easy and convenient to operate, short in detection time and the like, false positive results can be effectively reduced, the influence of positive control on a detection environment is reduced, and the primer group and the kit have high application value inrapid detection of the African swine fever viruses.

The invention relates to a kit and a detection method for polymorphism detection of a methylenetetrahydrofolate reductase gene. The kit comprises a nucleic acid extraction reagent and a nucleic acid detection reagent, wherein the nucleic acid detection reagent comprises fluorescent probe-containing PCR reaction liquid, a negative control and a positive control; the nucleic acid extraction reagent is a DNA extract; the PCR reaction liquid comprises DNA polymerase, UNG enzyme, a primer, a probe and Mg<2+>; the probe is a molecular beacon probe; and the gene polymorphic site of MTHFR is a C677T polymorphic site. The detection method using the kit is easy and fast to operate, the use of consumables is reduced so as to reduce the detection cost, the detection result is accurate, and the occurrence of false positive result is reduced; by adding the negative control and positive control in the amplification step, the judgment of false positive and false negative can be effectively improved, and the detection precision is higher; since the hairpin structure of the molecular beacon probe is opened with certain force, the specificity to the mutant C677T polymorphic site at the measuring point is higher.

The invention relates to a microfluidic-technology-based CTC protein typing kit. The kit comprises: (1) capture antibody compounds aiming at different CTCs marker protein, each capture antibody compound being made from a capture antibody in specific binding to corresponding marker protein and an aptamer, wherein the capture antibody and aptamer are connected through a cuttable material which is deoxyribonucleotide double strands having a palindrome structure and two or more than two restriction enzyme sites; and (2) a microfluidic chip, the surface of which is coupled to a cell capture assembly. The cell capture assembly made from a microcolumn is fixed at the surface of the microfluidic chip, and a specific conjugate capable of bonding to the aptamer modifies one end of the microcolumn. Through the microfluidic technology, efficient and fast automatic detection and typing of CTCs is achieved, the kit is simple to operate, false-negative and false-positive results due to man-made operational factors are reduced, and the sensitivity and accuracy of detection are improved.

The present invention discloses a strain of Lactobacillus salivarius capable of inhibiting Candida albicans growth, and a separation method thereof, wherein the Latin name is Lactobacillus salivarius LI01, the Lactobacillus salivarius LI01 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.7045. The present invention discloses morphological characteristics of the Lactobacillus salivarius LI01, wherein the morphological characteristics comprise rod-shaped bacteria, no spore production, no motility, and positive Gram staining. The present invention discloses main components of the whole-cell fatty acid of the Lactobacillus salivarius LI01, wherein the main components comprise: about 38.48% of 16:0, about 8.29% of 18:1w9c, about 14.68% of 19:0cyclow10c / 19w6, about 5.94% of 18:0, about 12.75% of 19:0cyclow10c / 19w6, about 11.55% of 19:0cyclow8c, about 12.75% of 19:1w6c, and about 14.68% of 18:1w7c. The invention further discloses the whole sequence of 16S ribosomal DNA (16SrDNA) of the Lactobacillus salivarius LI01. The separation screening method of the present invention comprises three steps: carrying out enrichment culture with a MRS nutrient culture medium, adopting a Candida albicans covering layer to carry out primary screening, and adopting liquid co-culture to carry out re-screening. According to the present invention, the method is rapid and efficient, and the obtained Lactobacillus salivarius LI01 has strong Candida albicans resistance ability.

The invention belongs to the field of analytical chemistry and the technical field of pesticide residue detection, particularly provides a method for separating and measuring enantiomers of chiral pesticides metalaxyl and dimethomorph with UPCC-MS / MS (ultraperformance convergence chromatography-tandem mass spectrometry) and relates to a separating and quantitating method for raceme of multiple chiral pesticides. According to the method, metalaxyl and dimethomorph in tobacco and dried fruits are extracted with a QuEChERS method, and the enantiomers of the two chiral pesticides metalaxyl and dimethomorph are synchronously detected through combination of chiral stationary phase of bonded phase chromatography and triple quadrupole MS. The convergence chromatography is adopted for the first time for rapid chiral separation of metalaxyl and dimethomorph, and sensitivity is high; supercritical CO2 is taken as a moving phase, so that a large quantity of organic solvents are saved, and the method is environmentally friendly.

An in-vitro micronucleus detection method for genetic toxicity of outlet water of a drinking water disinfection process is provided. The method includes: 1) a step of preparing a solution of an extract of the outlet water of the drinking water disinfection process; 2) a step of subjecting an isolated immortalized human embryonic kidney cell to exposure processing by utilization of the extract solution in the step 1); 3) a step of subjecting the cell processed in the step 2) to EMA staining and SYTOX Green dye staining in sequence; and 4) a step of analyzing with a flow cytometer. The detection method can reduce false positive results. Centrifugation with a pore plate is performed to exclude interference of fragments. The detection method can achieve rapid, micro and high-throughput analysis of the genetic toxicity of water samples of the drinking water disinfection process, and improve the sensitivity of the detection method. Detection results are prone to unification, good in reproducibility, accurate and reliable.

The invention relates to the technical field of biology, in particular to a combination, a kit and application of the kit. The application includes a solution of single-cell genome copy variation detection, comprising single cell amplification, library construction, high-throughput sequencing and information analysis; library construction based on transposase is applied to single cell sequencing;the use of single-tube one-step library construction process helps relieve operational complexity, thus avoiding pollution. In addition, the invention also provides optimal window length according tothe requirement on CNV (copy number variation) lowest detection resolution; run test is performed to count distribution differences, relative to coverage, of CNV area and normal area sequencing data;significance P value of each candidate CNV is output in final results; detection accuracy is effectively improved.

The invention relates to 'an aGST-ELISA detection method for bird flu viruses (H5N1 sub) serum antibodies', and its detection antigen is high- purity fusion protein GST-HA1 made by capturing fusion protein GST-HA1 on enzyme link plate by anti-GST monoantibody. And the method can be used to detect bird flu serum antibodies. And it has characters of simple antigen preparing process, high biosafety, low cost, easy to produce, etc, and lays the foundation of further development and research of bird flu detection reagent box.

The invention relates to a detection method for vitro cell micronucleus of cigarette smoke genetic toxicity, which is characterized in that immortalized human bronchial epithelial cells are used as target cells, the smoke condensate is exposed to toxicant, the cells undergo the low-permeability and high-permeability treatment, are dyed by two fluorescent probes and analyzed by a flow cytometer. Compared with the prior art, the detection method has the following advantages of: 1.overcoming the influence of apoptotic cells on the micronucleus detection and reducing false positive results. 2. overcoming the uncertainty of the invivo micronucleus detection (animal testing) due to the adoption of the vitro cell culture; 3.adopting the six pore plate cell culture and the flow cytometer detection which can meet the high flux micronucleus detection requirements; and 4. eliminating the disturbances of chondriosome and non-specific (fragments) particles and the like by the 2500*g gradient centrifugation. Therefore, the invention improves the sensibility of the detection method and the accuracy, the objectivity and the reliability of experimental results.

An aptamer-antibody method for detecting a trace of prostate-specific antigen (PSA) in blood is provided. A specific PSA aptamer coupled to magnetic particles and a specific PSA antibody coupled to nanogold particles are respectively mixed with a sample to be detected so as to detect, identify and / or quantify a target molecule (the PSA). The method is simple and convenient to use, sensitive, high in specificity, good in repeatability, rapid in result production, and free of needs for complex instruments and special techniques, and can be popularized to actual industrial application.

The invention provides a touch system and a signal processing method thereof. The signal processing method is used for matching a touch panel. The touch panel comprises a first sending area and a second sensing area. The first sensing area is monitored by at least one first sensor for generating a first monitoring result, and the second sensing area is monitored by at least one second sensor for generating a second monitoring result. The signal processing method comprises that whether a touched point formed in the first sensing area is adjacent to the second sensing area is judged. If the touched point is adjacent to the second sensing area, position information of the touched point is generated according to the first monitoring result and the second monitoring result.