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Method for identifying peptide by using tandem mass spectrometry data

A tandem mass spectrometry and data identification technology, applied in the field of proteome analysis, can solve the problems of unpredictable peptide fragmentation mode and wrong peptide identification, and achieve the effect of reducing false positive results and high accuracy.

Active Publication Date: 2006-05-10
INST OF COMPUTING TECH CHINESE ACAD OF SCI
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Problems solved by technology

In fact, the unpredictability of the peptide fragmentation method, the irrecoverable information lost during the fragmentation process, and the huge number of candidate peptides make random mismatches often occur, which may eventually lead to wrong peptide identification, that is, false positives. result

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  • Method for identifying peptide by using tandem mass spectrometry data
  • Method for identifying peptide by using tandem mass spectrometry data
  • Method for identifying peptide by using tandem mass spectrometry data

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Embodiment Construction

[0026] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0027] Such as figure 1 As shown, two amino acids can be connected by losing a water molecule to form a peptide bond at their C-terminal and N-terminal. A peptide is a sequence of amino acid residues connected to each other by peptide bonds. This sequence determines the identity of the peptide.

[0028] To identify the amino acid sequence of a peptide, the peptide is ionized and then passed into a mass spectrometer. In a mass spectrometer, peptide ions with a specific mass-to-charge ratio (m / z) (these peptide ions usually have the same amino acid sequence) are fragmented under the action of Collision-Induced Dissociation (CID). Under the action of low-energy CID, the rib bond can usually be broken in three ways to generate six series of fragment ions, namely a, b, c at the N-terminal and x, y, z series of fragment ions at the C-term...

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Abstract

The invention discloses a method for verifying peptide with tandem mass-spectrometric data which comprises steps of: splintering on the target peptide to generate trial tandem mass-spectrometric; theoretically splintering on a plurality of candidate peptides to generate multiple theoretical tandem mass-spectrometric; calculating the similarity of theoretical tandem mass-spectrometric and trial tandem mass-spectrometric with radial basic function which comprises an index; according to the calculated similarity, selecting the proper peptide which theoretical tandem mass-spectrometric is most similar with trial tandem mass-spectrometric as verified results. The inventive method has the advantages of having higher accuracy and lower false positive results.

Description

technical field [0001] The present invention relates to a proteome analysis method, in particular to a method for identifying peptide sequences. Background technique [0002] In current proteome research, protein identification based on tandem mass spectrometry is one of the most widely used techniques (references: Aebersold, R. and Mann, M. Mass spectrometry-based proteomics, Nature, 2003, 422: 198-207 ). One of the problems is how to automatically identify the peptide sequences that generate these spectra from experimentally obtained tandem mass spectra. To identify the sequences of the peptides from which experimental tandem mass spectra were generated, the method of database searching was widely employed (References: Eng, J.K., McCormack, A.L. and Yates, J.R. An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database. J Am Soc Mass Spectrom, 1994, 5: 976-989; Perkins, D.N., Pappin, D.J., Creasy, D.M. and Cottrell, J.S...

Claims

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Application Information

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IPC IPC(8): G01N33/48G01N23/00G01N30/72
Inventor 高文付岩李德泉孙瑞祥贺思敏杨强曾嵘周虎陈益强王晓彪
Owner INST OF COMPUTING TECH CHINESE ACAD OF SCI
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