47 results about "False-positive result" patented technology

The invention discloses a dry-type electrochemical luminescence (ECL) lateral flow chip and application thereof in CRISPR-Cas detection. The dry-type ECL lateral flow chip comprises a lateral flow test strip and an electrode plate, the lateral flow test strip comprises more than one detection test strip, one electric connection test strip and at least one quality control test strip; the test strip sequentially comprises an activation sheet, a combination sheet, a detection sheet, an absorption sheet and a common sample sheet, the test strip forms radial branches taking the sample piece as the center; the electrode plate comprises an integrated closed bipolar electrode and a driving electrode; the shape of the integrated closed bipolar electrode is the same as that of the lateral flow test strip. According to the invention, the CRISPR-Cas system is freeze-dried on the test strip, so that accurate multiple detection of pathogens can be realized; according to the method, the operation process is simplified, the detection time is greatly shortened, the operation error is reduced, the mutual interference among multiple detections is reduced, and the generation of false positive results is avoided.

The present invention provides the use of a reagent for detecting a target compound combination in the preparation of a detection agent, and the detection agent is used for screening and identifying whether a subject under test has taken drugs for treating vertigo, syncope or stroke, and assisting in determining whether the subject suffers from related diseases. Among them, the target compound combination includes one, several or all of lidocaine, cinepazide maleate, ticlopidine, penbutolol, fendiline, cinnarizine, clopidogrel, midodrine, dipyridamole, argatroban, rosuvastatin, atorvastatin, fluvastatin, and pitavastatin. The plasma sample pretreatment method of the present invention is simple, the detection and analysis time is short, the sensitivity of the method is high, and the types and quantities of detections are many; the method is divided into a primary screening method and a review method, which can improve the accuracy of the detection results and avoid false negative and false positive results.

Provided are compositions and methods useful to the determination of whether a microbial contaminant is present in a biological therapeutic production process. Specifically, an artificial positive amplification control plasmid and unique quantitative PCR detection probe are provided, which enables the rapid and real-time detection of a false positive result.

This invention belongs to the field of agricultural and aquatic organism detection technology, specifically relating to a fluorescent RPA detection kit and its application method for detecting Ichthyophthirius multifiliis. Compared to traditional PCR technology, the detection kit and method of this invention significantly simplify the operation steps. Furthermore, after RPA amplification, the results can be directly interpreted through real-time fluorescence signals, eliminating the need for subsequent analysis operations such as opening the lid or electrophoresis. This avoids cross-contamination between different amplification products and reduces false positive results, greatly improving detection efficiency and accuracy. In aquaculture, this innovative RPA kit will significantly enhance the rapid on-site detection capability of Ichthyophthirius multifiliis, providing an efficient, simple, low-cost solution that does not require cold chain transportation, meeting the urgent need of modern aquaculture for rapid and accurate pathogen detection.

The invention provides a feline herpesvirus antibody fluorescent microsphere detection test paper and a preparation method thereof, FHV gD protein is used as a specific capture antigen coating T line, feline IgG is used as a C line coating object, SPA and Eu (III) chelate particles are combined based on a lateral chromatography immunoassay method, and the fluorescent microsphere detection test paper for the feline herpesvirus antibody is obtained. The T-line fluorescence value, the C-line fluorescence value and the T / C value of the fluorescent microsphere detection test paper for the feline herpesvirus antibody are improved, accurate, rapid and quantitative indirect detection of the FHV antibody in feline serum is realized, and the sensitivity and the specificity of the detection test paper are improved. By testing a cat serum sample and a clinical sample, the detection result is consistent with that of a traditional standard method, and the reliability and effectiveness of the method in the aspect of on-site monitoring of the FHV antibody in the cat body are proved. Compared with a colloidal gold immunoassay method, generation of false positive results is avoided, and a feasible solution is provided for timely diagnosis and control of feline herpesvirus infection.

The present invention relates to the technical field of biological virus detection, and specifically discloses primers and probes for specifically detecting Cyprinid herpesvirus 2 and their applications. Among the primers and probes for detecting Cyprinid herpesvirus 2, the sequence of the inner primer FIP is as shown in SEQ ID NO:1, the sequence of the inner primer BIP is as shown in SEQ ID NO:2, the sequence of the outer primer F3 is as shown in SEQ ID NO:3, the sequence of the outer primer B3 is as shown in SEQ ID NO:4, and the sequence of the loop primer probe is as shown in SEQ ID NO:5. By using the primers and probes of the present invention, the accurate detection of Cyprinid herpesvirus 2 can be achieved, the appearance of false positive results caused by non-specific amplification can be avoided, and the lowest detection limit can reach 0.5 copies / μL, which is more conducive to the early detection of Cyprinid herpesvirus 2, thereby avoiding the spread of diseases, and has very important significance for the prevention and control of aquatic diseases.

Every year, approximately 94 million cases of Salmonella gastroenteritis, with 155000 deaths, are reported each year and 85% of them reported to be food-borne. Investigation of the foods whether they are clean for Salmonella and sensitivity, easy applicability, absence of false positivity and negativity and the speed are the features sought in the analysis method for this investigation. It is not desirable for analysis to detect the presence of dead bacteria in food. Although the final product does not contain microbiologically harmful live bacteria during the food process, the detection of dead bacteria transmitted before the process causes the food product to be unfairly diagnosed as harmful. To prevent this situation, the analysis kits depending on molecular methods, increase their microorganism detection levels up to to 104 while reducing their sensitivity. Since the molecular methods cannot discriminate dead and live organisms, a confirmation test is required to prove that the positive result of the analysis belongs to the live bacteria in the food, which results in additional cost and time loss. In the same way, it is necessary to verify whether the colonies that grow in the gold standard culture method, belong to Salmonella bacteria. In the developed system; 105 dead bacterial DNA is eliminated in the food to prevent false positive results and the minimum detection limit is 10 bacteria. Also, in developed system, 4 primers specific to 6 regions of DNA are used. Therefore, the specificity of the method is very high (99.9%) and no verification test is needed. Since PCR systems require a device with complex temperature control units, they can make analysis in a laboratory-dependent manner. In the proposed system, DNA is amplified at constant temperature; no temperature cycle is required, therefore no complex instrument and laboratory infrastructure are required. All the procedures can be easily performed outside the laboratory on a portable mini-heater where pre-enrichment, DNA isolation from the sample and PCR steps are performed. For molecular analyses, the device is required to display the result of imaging or analysis. In the developed method, DNAs amplified by the loop-mediated isothermal DNA amplification method, are hybridized and combined with the labeled probe and then can be read by lateral flow method with the naked eye. As the results are visible by eye, no additional device is required. The classical culture method is accepted as the gold standard, but the duration of analysis is 7 days for positive samples, 3 days with verification test, for the molecular methods, and 5.5 hours including pre-enrichment time in the developed system.

The present invention relates to improved methods for screening, diagnosing and / or monitoring colorectal advanced neoplasia (AN), advanced adenoma (AA) and / or colorectal cancer (CRC), wherein AN includes CRC and AA. In particular, the method provides increased specificity due to a reduction in false positive results in fecal occult blood tests (FOBTs) using characteristics based on bacterial markers. The present invention further relates to the use of the method in selecting subjects for exploratory testing (e.g., colonoscopy) or for treatment with anticancer therapy.

The invention relates to application of a Fe < 3 + >-ATP-CK cascade regulation type carbon dot fluorescent probe in dynamic monitoring of creatine kinase content, and relates to the field of new application of carbon dots. Fluorescence of B, N-CDs is quenched by Fe < 3 + >, and then fluorescence is recovered after ATP is added through specific binding of Fe < 3 + > and ATP. When CK catalyzes ATP to react with Cr, fluorescence is quenched again due to reduction of the ATP content. On the basis of high-selectivity competitive combination of Fe < 3 + > and ATP, interference of ADP, AMP and other analogues can be eliminated. The multiple verification mechanisms of Fe < 3 + > quenching, ATP recovery and CK re-quenching significantly reduce the false positive result, and enhance the detection reliability. A portable sensing platform constructed based on a smart phone establishes a new method for bedside detection of CK, establishes a brand new strategy for CK detection, and shows a wide prospect in rapid screening application of clinical diagnosis of myocardial infarction and the like.

The invention discloses a POCT (point-of-care testing) system and a method thereof, and relates to the technical field of biological medicine. The present invention comprises: a sample processing module for processing a plurality of sample types, including swabs, blood, milk and urine; the nucleic acid amplification module adopts an enzymatic isothermal amplification (ERA) technology; the target recognition module is used for carrying out nucleic acid recognition by utilizing a CRISPR / Cas12a system and has specificity of a single base level; and the result reading module can realize visualization of nucleic acid reaction signals and automatically judge whether the nucleic acid reaction signals are negative or positive. According to the present invention, a variety of animal infectious disease pathogens can be simultaneously detected, the detection requirements in the actual production can be met, the detection sensitivity is high, the low-concentration pathogen nucleic acid can be detected within the short time, the specificity is good, the false positive result can be effectively avoided, the operation is simple, the automation degree is high, the operation is not required by the professional technician, and the cost is low. The kit is suitable for on-site rapid detection, and provides powerful support for early diagnosis and prevention and control of animal infectious diseases.

The utility model relates to the technical field of gene detection, in particular to an EGFR (epidermal growth factor receptor) gene mutation multiple detection kit which comprises a kit body and a kit cover connected to the kit body, a fixing plate is fixedly arranged in the kit body, a plurality of first placing holes and a second placing hole are formed in the fixing plate, sponge rings are fixedly arranged in the first placing holes, and the sponge rings are fixedly arranged in the second placing holes. A reagent tube filled with a fluorescence labeling probe is arranged in the sponge ring, a sample tube is arranged in the second placing hole, and a sample dripping mechanism for adding a sample into the reagent tube in a dripping manner is further arranged on the fixing plate in a sliding manner; the sample dripping mechanism comprises a sliding block arranged on the fixing plate in a sliding manner, a fixing frame is fixedly arranged on the side wall of the sliding block, and a sample dripping hopper is fixedly arranged at the top of the fixing frame; according to the EGFR gene mutation multiple detection kit, a sample can be controlled to be dripped into the reagent tube in a dripping manner, so that the pouring amount of the sample is accurately controlled, and false negative or false positive results caused by non-uniform pouring of the sample are avoided.

The invention discloses a preparation method of a monoclonal antibody for detecting trichophyton rubrum and application of the monoclonal antibody in fungal skin disease detection, and belongs to the field of biological medicine. The monoclonal antibody provided by the invention only has specific reaction with trichophyton rubrum, and has no cross reaction with common fungi such as trichophyton mentagrophytes, epidermophyton flocculent, candida albicans, malassezia and the like and normal flora of a human body, so that a false positive result is effectively avoided. Based on the double-antibody sandwich principle, the detection sensitivity can reach 200 pg / mL level, trace trichophyton rubrum in a clinical sample can be detected, and the positive rate is obviously higher than that of direct microscopic examination. The whole detection process can be completed within 15-20 min and is far faster than fungus culture for several weeks, a rapid diagnosis basis can be provided for doctors, and early treatment is facilitated. The kit provided by the invention has standardized operation steps, does not need complex instruments and special training, and is suitable for popularization and use in clinical laboratories of hospitals at all levels, outpatient clinics of dermatology and even basic medical institutions.

The present invention provides a primer set, a modified primer set for detecting porcine pathogenic coronaviruses and their applications, belonging to the technical field of inspection and quarantine. The primers designed for PEDV and PDCoV respectively by the present invention have the advantages of high accuracy, moderate sensitivity and good specificity, and can be used in combination with microfluidic and colloidal gold methods. By using microfluidic technology, the present invention completes the amplification of the target fragment in a chip, and finally realizes the visualization result through colloidal gold technology, featuring convenience, simplicity, high sensitivity and good repeatability. The reaction of the present invention is completed in one step from RNA to the end of amplification in the reaction cavity of the microfluidic chip, eliminating the two-step reaction from reverse transcription to amplification, reducing the contamination caused by opening the lid and avoiding false positive results. In addition, the two primer sets of the present invention can simultaneously perform PCR reactions in the same chip, enabling the detection of two porcine pathogenic coronaviruses, namely PEDV and PDCoV, in the same sample.

The application discloses a biomarker for prognosis evaluation of extrahepatic cholangiocarcinoma, a prognosis risk evaluation system and application, and belongs to the technical field of bioinformatics. The application adopts the combination of miR-34c-5p, miR-100-5p, miR-193b-5p, miR-122-5p, miR-5588-5p and miR-122-3p as a biomarker, can effectively improve the accuracy of differential diagnosis of extrahepatic cholangiocarcinoma, can distinguish extrahepatic cholangiocarcinoma patients from healthy people and benign biliary disease patients with high precision, and overcomes the limitation that traditional tumor markers such as CA19-9 are prone to false positive results in benign biliary diseases. Meanwhile, the expression level change of the biomarker combination can dynamically reflect the postoperative tumor load change, and is helpful for accurately evaluating the surgical effect.

The invention discloses a primer pair, a kit and a system for detecting echinococcosis multilocularis based on multiple PCR-CRISPR / Cas12a of circulating free DNA (deoxyribonucleic acid), and application of the primer pair, the kit and the system, and relates to the technical field of gene detection. According to the primer pair for detecting the echinococcosis multilocularis based on the multiple PCR-CRISPR / Cas12a of the circulating free DNA, the 1260 gene, the Nad5 gene and the U1 gene of the echinococcosis multilocularis serve as targets, and the PCR primer pair comprises Em-1260-F4R3, Em-NAD5-F3R5 and Em-U1-F4R1. According to the invention, rapid and sensitive detection can be realized through a multiple PCR-CRISPR / Cas12a detection system, complicated equipment and operation are not needed, and the occurrence rate of false positive results is also reduced.

The invention discloses a reusable dual-mode aptamer biosensor for detecting clostridium perfringens, which is characterized by comprising a competitive chain CP-C, a CeO2 (at) PANI nano composite material, a closed bipolar electrode system and an aptamer functionalized magnetic bead probe. According to the invention, the high-affinity nucleic acid aptamer is combined with the specific extracellular electron transfer mechanism of the clostridium perfringens viable bacteria for the first time, a new viable bacteria detection sensing strategy free of nucleic acid extraction and target gene amplification is constructed, and the biosensor can specifically recognize the viable clostridium perfringens, and can be used for detecting the viable bacteria of the clostridium perfringens. According to the present invention, with the detection method, the false positive result caused by the existence of dead bacteria is avoided, the detection can be rapidly completed within 3 min, the voltage signal and the G value signal are mutually proved, the false positive or false negative of the single signal detection is effectively avoided, the detection limit is as low as 2.50 CFU / mL, and the excellent anti-interference ability in the complex food matrix such as chicken and milk is provided; the core element ITO glass electrode can be continuously and repeatedly used for 12 times, and the performance is not obviously attenuated.

This application belongs to the field of immunoassay technology, specifically relating to specific binding proteins of hepatitis B viruses (MHBs), nucleic acid molecules, vectors, cells, detection kits, and their preparation methods and applications. This application provides a specific binding protein of MHBs with specific CDRs, exhibiting high affinity and specificity. It solves the problem in existing technologies of being unable to distinguish between large, medium, and small hepatitis B virus proteins, and ensures that the antibody does not cross-react with large and small proteins, thus improving detection specificity and avoiding false positive results. The specific binding protein of MHBs in this application can bind to a chemiluminescent detection system, thereby achieving highly sensitive detection of MHB antigen levels in clinical samples, with high consistency with nucleic acid detection results, providing an important tool for monitoring hepatitis B infection status and disease progression.

This application belongs to the field of immunoassay technology, specifically relating to LHBs-specific binding proteins, nucleic acid molecules, vectors, cells, detection kits, and their preparation methods and applications. This application provides an LHBs-specific binding protein with specific CDRs, exhibiting high affinity and specificity. It solves the problem in existing technologies of being unable to distinguish between large, medium, and small hepatitis B virus proteins, and ensures that the antibody does not cross-react with medium and small proteins, thus improving detection specificity and avoiding false positive results. The LHBs-specific binding protein of this application can bind to chemiluminescent detection systems, thereby achieving highly sensitive detection of LHBs antigen levels in clinical samples, with high consistency with nucleic acid detection results, providing an important tool for monitoring hepatitis B infection status and disease progression.

The invention provides a multisystem disease analysis method based on gastrin plasma proteomics, which comprises the following steps of: A, analyzing a large number of adult individuals subjected to baseline evaluation by using queue research data of a biological sample library, and measuring a plurality of plasma proteins; b, analyzing a standardized protein expression (NPX) value of gastrin (GAST) by adopting a multivariable Cox proportional risk model, and correcting by using Bonferroni so as to reduce a false positive result; and C, exploring the correlation between the GAST-PRS and the disease phenotype by adopting full phenotype correlation analysis (PheWAS) based on polygene risk score (PRS), and further analyzing the genetic correlation between the GAST and the disease by using linkage imbalance score regression (LDSC). According to the method, the GAST can be redefined as a cross-system risk regulation factor, and the method is of great significance to layering of patients with shared pathway imbalance. The GAST has multi-system correlation with morbidity and mortality in the outcome of 168 diseases, and challenges the classical normal form of the gastrointestinal specific function of the GAST. These findings establish the location of GAST as a multi-system risk indicator.

The invention relates to the technical field of in-vitro diagnosis, in particular to a kit for detecting an allergen-specific IgE (Immunoglobulin E) antibody. The kit comprises an anti-interference agent used for being mixed with a to-be-detected sample, an immunolabelled allergen, a peroxidase compound capable of being combined with an immunolabel and catalyzing color development of a substrate, and the substrate capable of being catalyzed by peroxidase to develop color, the anti-interference agent is prepared from the following components in parts by weight: 3 to 6 parts of polystyrene microspheres, 12 to 18 parts of L-glucoside and 6 to 10 parts of casein. The kit provided by the invention can effectively reduce false positive and false positive results, so that the accuracy of the detection result is improved.

The invention relates to a method and a product for preventing false positive results in real-time fluorescent quantitative PCR detection of pathogens. The positive reference template provided by the invention is used for replacing a positive control template, so that a false positive result caused by the fact that a test sample is polluted by the positive control template in real-time fluorescent quantitative PCR detection of pathogens is prevented.

The invention describes a recombinant Mycobacterium strain such as M. smegmatis that is recombinantly converted to express the M. tb complex ESX protein expression system, e.g. M. tb complex ESX-1 protein expression system. The recombinant strain exports virulence effector proteins (such as EspA, EspB, EspC, EsxA or EsxB) similar to M. tb, when administered to a subject, and is therefore useful as a potential tuberculosis vaccine candidate. The recombinant strain is non-pathogenic and the vaccinated subject does not give a false positive result when tested with any standard tuberculosis diagnostic test e.g. CCT, as the recombinant strain does not sensitize the vaccinated host to M. tb antigens or protein derivate of M. bovis.

The invention discloses a digestive tumor inflammation pathology dynamic detection and targeted therapy method, and relates to the technical field of biological sample detection analysis, and the method comprises the following specific steps: 1, collecting tissue samples and blood samples from digestive tumor inflammation patients, non-tumor inflammation patients and healthy people, the method has the advantages that tissue and blood samples are collected from a large number of digestive tumor inflammation patients, non-tumor inflammation patients and healthy people, the specific recognition molecules are specifically combined with the digestive tumor inflammation specific markers in the samples, and the signal intensity is detected through the fluorospectro photometer; according to the method, the content of the marker is obtained through comparison with a standard curve, the illness state is judged according to a threshold value, the whole process is linked with one another, specific recognition and combination of unique biomarkers related to digestive tumor inflammation are achieved from sample collection to result judgment, false positive results are effectively reduced, and the accuracy of clinical diagnosis is remarkably improved.

The present invention relates to the technical field of animal disease detection, and specifically discloses primers and probes for detecting Mycoplasma gallisepticum by LAMP-Taqman and their applications. Among the primers and probes for detecting Mycoplasma gallisepticum, the sequence of the inner primer FIP is as shown in SEQ ID NO:1, the sequence of the inner primer BIP is as shown in SEQ ID NO:2, the sequence of the outer primer F3 is as shown in SEQ ID NO:3, the sequence of the outer primer B3 is as shown in SEQ ID NO:4, the sequence of the loop primer LF is as shown in SEQ ID NO:5, and the sequence of the loop primer probe is as shown in SEQ ID NO:6. By using the primers and probes of the present invention, the accurate detection of Mycoplasma gallisepticum can be achieved, and the method can avoid the occurrence of false positive results caused by non-specific amplification, with strong specificity, high sensitivity, and the lowest detection limit reaching 0.258 fg / μL, providing a reliable guarantee for the early clinical detection of Mycoplasma gallisepticum.

The application discloses a primer probe combination, a kit and application for detecting Nocardia, a mycobacterium tuberculosis complex and mycobacterium, and belongs to the technical field of genetic engineering. The technical problem to be solved is that there is a lack of a nucleic acid detection tool for Nocardia, the mycobacterium tuberculosis complex and mycobacterium in the prior art, which is high in sensitivity, high in specificity and simple in operation. The technical solution is characterized in that the primer probe combination comprises a primer probe for detecting Nocardia, a primer probe for detecting the mycobacterium tuberculosis complex, a primer probe for detecting mycobacterium and a primer probe for detecting an internal standard control. The primer probe combination can simultaneously screen Nocardia, the mycobacterium tuberculosis complex and mycobacterium, quickly and simply confirms pathogenic species, saves time, manpower and reagent cost, and has the advantages of high specificity, a low minimum detection limit and prevention of false negative or false positive results.

A tumor stroma imaging agent with a chemical structural formula (I):is provided, where R is hydrogen or fluorine. Compared with the prior art, the tumor stroma imaging agent exhibits significant affinity for fibroblast activation protein (FAP), high uptake for a malignant tumor with high FAP expression in a tumor stroma, and high sensitivity and specificity for the diagnosis of a malignant tumor, and is not prone to false positives. Therefore, the tumor stroma imaging agent can be effectively and safely used for the diagnosis and treatment of various malignant tumors with a prolonged half-life and an extended window period, which is conducive to clinical application.

The invention provides a near-infrared AIE fluorescent probe as well as a preparation method and application thereof. The fluorescent probe has obvious AIE property, aggregation-induced emission (AIE) effect and good light stability, can realize seven-day imaging of CAR-T cells, can target CAR-T cell mitochondria, cannot leak to other cells, and avoids false positive results. Meanwhile, the probe has good biocompatibility, does not influence CAR-T cells to secrete key cell factors IFN-gamma, and does not damage the killing function of the CAR-T cells on tumor cells. In a small animal living body level, the probe N-6 is used for tracing CD19 CAR-T cells in a Raji subcutaneous tumor model mouse, a result shows that a fluorescence signal can be continuously observed for 7 days, and the CAR-T cells are continuously enriched in tumor tissues and liver tissues, which indicates that the probe N-6 can monitor the dynamic distribution process of the CAR-T cells in vivo in real time.

The invention belongs to the technical field of biological medicine, and particularly relates to a kit for detecting a breast cancer methylation marker and application of the kit. The kit provided by the invention has a better detection effect on four types of breast cancer by detecting the methylation level of a combination of the ENPP2 gene and the NPTX2 gene in a sample, and the detection sensitivities of the kit to breast cancer types with higher malignancy and poor prognosis of three-negative and HER-2 positive tumors can reach 94.29% and 93.75% respectively; the kit has relatively high detection specificity for mammary gland fibroma patients, mammary gland hyperplasia patients, mammary cyst patients and healthy people, detection of false negative and false positive results can be effectively avoided, and the detection accuracy is high.

The invention discloses a potato scab farinae molecular detection marker and a detection method thereof, the molecular marker is a genome DNA fragment with the length of 2496bp, and the nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 1. Based on the molecular marker, a primer pair, SsF1 / R1, SsF2 / R2 and / or SsF3 / R3, for identifying the molecular detection marker for potato scab farinae is also designed. The detection marker has extremely high species specificity, can accurately identify the potato scab farinae, effectively avoids false positive results, greatly improves the accuracy and reliability of detection, provides powerful technical support for rapid and accurate detection of potato scab farinae, is helpful for taking prevention and control measures in time, and has a wide application prospect. The loss of the potato industry caused by diseases is reduced.