194 results about "Respiratory virus" patented technology

The invention provides methods of preventing and/or treating infection by a respiratory virus such as respiratory syncytial virus (RSV) and SARS-associated coronavirus, particularly reducing infection and/or one or more symptoms of respiratory virus infection. A polynucleotide comprising an immunostimulatory sequence (an "ISS") is administered to an individual which is at risk of being exposed to a respiratory virus, has been exposed to a respiratory virus or is infected with a respiratory virus. The ISS is administered without any antigens of the respiratory virus. Administration of the ISS results in reduced incidence and/or severity of one or more symptoms of respiratory virus infection.

The present disclosure relates to novel compounds of formulas (1) through (19) and to a method for treating humans infected with a virus including various respiratory viruses such as members of the Paramyxoviridae family (respiratory syncytial virus (RSV), human metapneumovirus (HMPV), human parainfluenza virus (HPIV), measles virus, and mumps virus) with a compound of formulas (1) through (19). The present disclosure also relates to a cytopathic effect (CPE)-based assay that will assess virus-induced CPE for screening of compounds for treating viral diseases or inhibiting a virus.

The invention belongs to the technical fields of biochips and diagnostic reagents, and discloses a method for detecting various respiratory viruses, and primers and probes thereof. In the invention, nucleotide sequences of 14 respiratory viruses, namely adenovirus, human metapneumovirus, influenza virus A, influenza virus B, respiratory syncytial virus, bocavirus, rhinovirus, coronavirus (HKU1, NL63 and SARS), and parainfluenza virus (type I, type II, type III and type IV) are analyzed, and corresponding reverse transcription primers, PCR primers and specific probes are designed. Specific gene segments are amplified by reverse transcription and multiple asymmetric PCR methods; a fluorescence-coded microsphere group coupled with the virus specific probes and the PCR amplification product are incubated and hybridized by liquid phase chip technology; and finally the Bio-PlexTM200 is used for detection. The detection method has the advantages of high flux, high specificity and sensitivity, stable results and good repeatability, the detection method is easy to operate, and the detection speed is high.

The present invention relates to new methods and nucleic acid sequences for the detection and quantification of human metapneumovirus.

The invention discloses a method for simultaneously detecting twelve kinds of common respiratory viruses. According to the method, primers and probes are designed according to gene conservative areas of the twelve kinds of common respiratory viruses, namely influenza A virus, influenza B virus, influenza C virus, parainfluenza virus type 1, parainfluenza virus type 2, parainfluenza virus type 3, rhinovirus, Bocavirus, adenovirus, coronavirus, metapneumovirus and respiratory syncytial virus, nucleic acid fragments of samples to be measured are extracted for amplifying, and finally, the samples are separated by using a capillary electrophoresis method. The method disclosed by the invention has the advantages of low required sample size, high sensitivity and accuracy, good specificity and low cost; the defects that the conventional single tube multiplex fluorescence PCR (Polymerase Chain Reaction) detection primers are difficult to design, and multicolor fluorescence mutually intervenes and is not easy to part are overcome, the defects that a chip detection method is tedious in operation, high in detection cost and the like are also overcome, and a new method is provided for screening the respiratory viruses.

The invention provides a kit for nucleic acid combined detection of the influenza virus A, the influenza virus B and the respiratory syncytial virus. The kit comprises RT-PCR reaction liquid, an RT-PCR enzyme mixture, a respiratory virus primer probe, internal reference, negative contrast, clinical positive contrast and strong positive contrast. The one-step RT-PCR reaction can be directly conducted on the well-extracted respiratory virus nucleic acid, the influenza virus A, the influenza virus B and the respiratory syncytial virus in a sample can be detected in a classified and qualitative mode, the gene of the internal reference serves as the internal contrast, and contamination is prevented through UNG enzymes. The kit is simple in one-step amplification method, short in procedure, easy and convenient to operate, capable of preventing contamination, high in detection result specificity, high in sensitivity, clear in result, high in credibility, and capable of being used for qualitatively authenticating and detecting the influenza virus A, the influenza virus B and the respiratory syncytial virus in a human nose pharynx-mop sample.

The present invention provides for the use of iota- and/or kappa-carrageenan for the manufacture of an antiviral pharmaceutical composition for the prophylaxis or treatment of a pathological condition or disease caused by or associated with an infection by a respiratory virus selected from the group consisting of orthomyxovirus, paramyxovirus, adenovirus and coronavirus. The present invention further provides for the use of fucoidan, in particular of high molecular weight fucoidan, for the manufacture of an antiviral pharmaceutical composition for the prophylaxis or treatment of a pathological condition or disease caused by or associated with an infection by a respiratory virus selected from the group consisting of orthomyxovirus and paramyxovirus.

The invention relates to the field of medical detection, in particular to a primer probe combination for detecting six respiratory viruses, a kit and application. According to the kit provided by theinvention, a method of combining isothermal amplification and a micro-fluidic chip is adopted; the kit can accurately detect novel coronavirus (2019-nCoV) S and N target genes and influenza A virus, novel influenza A H1N1 virus (2009), influenza A H3N2 virus, influenza B virus and respiratory syncytial virus at the same time, and the purpose of distinguishing the novel coronavirus from common influenza virus is achieved. The kit disclosed by the invention has the advantages of multiple detection indexes, simplicity and convenience in operation, short detection time and the like, six virus indexes can be simultaneously detected by one-time sample adding, the time from sample treatment to report is within 1.5 hours, and the kit is more time-saving and labor-saving than qPCR method.

The present invention relates to a kit for simultaneous diagnosis of viral respiratory diseases. To be more specific, the present invention is directed to a method for diagnosing viral respiratory diseases by detecting the genes specific to the respiratory disease-causing virus, a primer set for diagnosing the viral respiratory diseases used in the diagnosis method, a composition for simultaneous diagnosis of viral respiratory diseases, comprising the primer set, and a kit for simultaneous diagnosis of viral respiratory diseases, comprising the composition. When the primer set of the present invention for diagnosing the viral respiratory diseases is used, 14 different types of respiratory viruses can be simultaneously detected only with one reaction through real-time multiplex reverse transcription (RT)-PCR, and the onset of respiratory diseases caused by these viruses can be diagnosed. Thus, the primer set of the present invention can be widely used for prompt diagnosis and treatment of respiratory diseases.

The invention belongs to the biotechnology application field, and relates to simultaneous detection and genotyping of infections of 16 respiratory viruses (including FluA, FluB, sH1N1, PIV1, PIV2, PIV3, RSVA, RSVB, HRV, HMPV, HBoV, CoV NL63, CoV OC43, CoV 229E, CoV HKU1 and Adv) of nasopharyngeal extract specimens of patients of respiratory-related diseases of all levels of disease prevention and control institutions and sentinel hospitals. Specifically, nucleotide sequences of representative strains of the 16 respiratory viruses are downloaded from NCBI; through literature review and multiple sequence alignment, pathogen relatively-conservative regions are determined, and multiplex specific primers are designed. Single-tube multiplex (18 stages) PCR detection is carried out for detecting the 16 respiratory virus conservative regions, and an entire reaction takes less than 2 hours. According to the invention, a defect that genotyping cannot be carried out with conventional single-tube multiplex fluorescent qualitative PCR detections can be overcome, and defects of complicated operations, long time, and high cost of conventional chip detection methods can be overcome. With the application provided by the invention, a novel idea is provided for respiratory virus genotyping technologies. With characteristics of high specificity, high sensitivity, and high speed, powerful technological support is provided for rapid and accurate screening and genotyping of the respiratory-disease-related viruses. The invention has important significance upon the researches of respiratory-tract patient infection pathogen spectrum of out nation, and upon molecular epidemiological investigations.

The invention provides a primer combination for detecting various respiratory viruses, a specific probe combination for detecting various respiratory viruses, a method using the primers and probe to detect various respiratory viruses and a kit comprising the primer combination and the probe combination. In the invention, the mixed solution of the specific primers and the probe with fluorescent mark is utilized to carry out PCR (Polymerase Chain Reaction) amplification on batch samples, and then microsatellite analysis is performed, so as to analyze a great quantity of samples at one time and detect various respiratory viruses simultaneously, thus significantly improving efficiency and realizing high sensitivity. The kit can be popularized and used clinically, and has wide application prospect.

The invention discloses a multiplex PCR (Polymerase Chain Reaction) assay method for simultaneously assaying multiple respiratory viruses, which includes the following steps: design of probes, design of upstream and downstream primer sequences, multiplex PCR reaction and dissociation curve analysis. By designing Taqman probes for the conserved group sequences of the respiratory viruses and adopting different fluorophores when Tm phase difference is less than or equal to 6 DEG C, the method can assay a variety of respiratory viruses with high throughput and high accuracy. The invention further provides a multiplex PCR assay probe set and a corresponding kit for simultaneously assaying multiple respiratory viruses, which are convenient to apply and have a great market and application prospect.

A self-service respiratory vaccination instrument for SARS infectious disease belongs to respiratory tract communicable disease treating, defending and spread proof medical apparatus. The self-service respiratory vaccination instrument uses instruments means to create high temperature as in summer, and uses the penetrating power of damp-heat water vapour, or the destructive power to the virus structure from negative oxygen ion and pH regulating agent, or the killing power to the microorganism from plant essence oil to make the respiratory tract virus, pathogenic bacteria such as 'SARS' to be attenuated, killed or inactivated; the microorganism attenuated, killed or inactivated in the respiratory tract and its giblets become specificity vaccine for the microorganism and are disposed in the respiratory tract, the effect that the 'SARS' respiratory tract microorganism vaccine can be inoculated to the respiratory tract with no time difference and no other outer vaccine can be realized, and the object of the cure, defense and diffuse proof for corresponding respiratory tract infectious diseases can be achieved.

The invention provides a respiratory virus sample collection and detection integrated device. A detection card is arranged on a wearing device, droplets are collected by protective equipment to be used as a sample for direct detection, protection is integrated with sample collection and detection, and viruses in the droplet sample can be automatically detected; manual throat swab sampling and detection operation is not needed, so on the one hand, a large amount of manpower and material resources can be saved, and meanwhile, the detection speed is remarkably increased; on the other hand, directcontact between medical staff and the droplets of the patient is effectively reduced, the mask can also be used as a common mask for isolating the droplets in the sampling detection process, and theinfection risk in the sampling process is greatly reduced. The device is suitable for rapid and efficient diagnosis of a large number of to-be-detected personnel, is especially suitable for rapid screening of a large number of suspected cases and closely contacted persons of definite cases, and can effectively relieve the pressure of detection and diagnosis work.

The invention discloses an andrographolide derivative and a preparation method and application thereof, belongs to the technical field of medicines, and relates to a novel compound with respiratory virus activity resistance, an isomer of the compound or salt capable of being accepted pharmaceutically, a medicine composition containing the compound, a preparation method of the medicine composition and application of the compound in the medicine for treating and/or preventing infections caused by respiratory viruses. The compound and the isomer of the compound or the sat capable of being accepted pharmaceutically are shown in the general formula (I), wherein R1, R2 and R3 are specified in the patent claim and the specification.

The invention discloses LAMP (loop-mediated isothermal amplification) primer combination for detecting six respiratory viruses, and the application thereof. The primer combination provided by the invention consists of 36 DNA molecules as shown in the sequence 1 to the sequence 36. The primer combination can be used for detecting whether a to-be-detected virus is influenza A virus subtype H1N1, influenza A virus subtype H3N2, avian influenza virus subtype H5N1, avian influenza virus subtype H7N9, influenza B virus or human adenovirus, and can be used for detecting whether a to-be-detected sample contains influenza A virus subtype H1N1 and/or influenza A virus subtype H3N2 and/or avian influenza virus subtype H5N1 and/or avian influenza virus subtype H7N9 and/or influenza B virus and/or human adenovirus. The primer combination provided by the invention is used for detecting six respiratory viruses, has high specificity and high sensitivity, and can realize simple, convenient, rapid and accurate detection. A great promotional value is achieved.

The invention discloses an mRT-PCR (multiple reverse transcription-polymerase chain reaction) kit for discriminating and detecting important avian economic animal viruses and an application of the mRT-PCR kit. The kit comprises primer pairs for detecting an AIV (avian influenza virus) M gene, AIV H7,H9 and H5 subtypes, an NDV (newcastle disease virus), an ILTV (infectious laryngotracheitis virus), an IBDV (infectious bursal disease virus) and an EDS (egg drop syndrome) virus. The mRT-PCR kit has the characteristics of high sensitivity, high specificity and the like when used for detecting AIVs and other common respiratory viruses, can be used for immediately detecting and discriminating important and popular subtypes of the AIVs and five avian respiratory pathogens most popular in China and has a great significance in zoonosis control, monitoring, prevention and control of avian mixed infection, biosafety of farms, reduction of loss of the breeding industry, food safety, quarantine and the like.

The invention relates to a respiratory virus detection kit, and simultaneously provides a respiratory virus detection method using the kit. The kit contains 8 groups of primers and probes thereof, wherein in the primers, the 5' ends of the downstream primers are marked by using biotins. The detection method comprises the following steps of: amplifying nucleic acid in a sample to be detected by using the primers, and hybridizing the formed biotinylation products and the probes fixed on the surface of a chip to form a probe-biotinylation product complex; combining an alkaline phosphatase-streptavidin conjugate and the probe-biotinylation product complex, and performing color (bluish violet) generation reaction on nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl-phosphate (NBT/BCIP) serving as a color development substrate and alkaline phosphatase; and detecting whether the sample to be detected contains respiratory viruses to be detected by judging whether the specific site develops color. The kit has high detection flux, is quick and sensitive and has strong specificity and very strong practicability.

Peptides having activity of inhibiting infections of respiratory viruses and use of the same are disclosed. The peptides can be synthesized by chemical or genetic engineering methods, and they have functional domain capable of binding to surface glycoprotein of respiratory viruses and activity of inhibiting infections of respiratory viruses. The peptides are useful for blocking infections of respiratory viruses in target cells, for prevention/treatment of said infections, and for development of new prophylactic/therapeutic medicaments against respiratory viruses. Also disclosed are a kit for screening peptide capable of inhibiting said infections and the screening method. The invention also discloses the mechanism of the peptides in inhibition of said infections, which provides theory support for developing new prophylactic/therapeutic agents with broad-spectrum antiviral activities.