15 results about "Respiratory virus" patented technology

The present disclosure relates to methods for treating or preventing immune-mediated pulmonary injury (IMPI) in a subject in need thereof, the method comprising administering to the subject an anti-Type I interferon (anti-T1i) therapy that blocks the activity, function or production of Type I interferon (T1i) in the subject, wherein the anti-T1i therapy is administered to the subject infected with a respiratory virus that causes a delayed T1i response in the subject.

This application relates to the field of pharmaceutical technology, specifically disclosing a self-sampling mouthwash wash composition, a self-sampling mouthwash wash, and its application. The self-sampling mouthwash wash composition, based on the self-sampling mouthwash wash composition, comprises 0.1–2.5 wt% sodium hexadecyl sulfonate, 0.05–1.0 wt% guanidine derivative, and 0.05–1.0 wt% amino derivative. The raw materials of the self-sampling mouthwash wash include the self-sampling mouthwash wash composition. The application of the self-sampling mouthwash wash in oral self-collection of respiratory viruses. This application improves the elution ability of respiratory viruses, bacteria, and other pathogenic microorganisms in the oral cavity during self-sampling.

This invention belongs to the field of ferret health examination technology, and particularly relates to an integrated method and device for ferret respiratory virus challenge and dynamic body fluid sampling. It includes: a base plate with a sliding groove inside, a telescopic plate slidably installed within the sliding groove, support arms fixedly installed on both sides of the base plate and the telescopic plate, an arc-shaped plate fixedly installed at the top of each support arm, a sponge pad fixedly installed on the inner side of the arc-shaped plate, a clamping plate rotatably installed on the top of the arc-shaped plate, a second sponge pad fixedly installed on the inner side of the clamping plate, a protrusion fixedly installed on the arc-shaped plate, a sliding groove inside the protrusion, symmetrically sliding limit blocks installed within the sliding groove, one end of each limit block abutting against the clamping plate, and a spring fixedly installed between the two limit blocks. This invention has a wide range of applications, allowing for rapid adjustment of the device length and support arm spacing according to the height and size of the ferret, adapting to different sizes of experimental ferrets, effectively reducing animal stress and injury, and providing convenient, safe, and reliable fixation operation.

Provided in the present invention are a method and a kit for multiple detection of respiratory virus nucleic acids, and in particular, disclosed are a method, a primer, a probe and a kit for detecting a plurality of influenza A viruses such as H1N1(2019), H3N2, H5N1, H1N1 and H7N9, influenza B viruses such as Yamagata and Victoria, 2019-nCoV OFR1ab and N genes, and human internal standard gene GAPDH on the basis of a real-time fluorescent quantitative PCR technical platform.

The application belongs to the technical field of targeted adenovirus, and particularly relates to a nanobody specifically combined with adenovirus Fiber protein and application thereof, and the nanobody 252A7, an amino acid sequence of which is shown as SEQ ID NO. 2. The nanobody 252A7 provided by the application can specifically recognize and combine with Fiber proteins of various serotypes of adenovirus (such as HAdV-3, HAdV-4, HAdV-7, HAdV-55), solves the technical bottleneck that traditional antibodies are difficult to recognize the conserved epitope of Fiber protein and lack broad spectrum, can effectively distinguish adenovirus from other common respiratory viruses (such as RSV, SARS-CoV-2), avoids cross reaction, and improves the accuracy and reliability of clinical etiology diagnosis. The nanobody of the application can be used for developing high-sensitivity immunological detection reagents (such as ELISA, immunochromatography, chemiluminescence, etc.), and has potential application value in the fields of targeted therapy and molecular imaging.

The application discloses a paper-based sensor chip for multiple respiratory virus detection and a preparation method and application thereof. The paper-based sensor chip takes filter paper as a base material and is provided with a sample adding area (100), a working electrode area (200), a reference electrode area (300) and a counter electrode area (400). The working electrode area (200) is provided with a plurality of working electrodes which are arranged in a row matrix in a direction away from the sample adding area (100). The working electrode takes graphene as a conductive channel, and the surface of the working electrode is fixed with a nano-gold-streptavidin composite material and a capture probe covalently combined with biotin. The upper side of the working electrode is the reference electrode area (300), the lower side of the working electrode is the counter electrode area (400), and the working electrode, the reference electrode area (300) and the counter electrode area (400) form a three-electrode system. The paper-based sensor chip can realize efficient and synchronous detection of multiple viruses and has good application value.

THIS invention relates to the synthesis of derivatives of siphonochilone and therapeutic use thereof in the treatment and CN prevention of respiratory virus disease, in particular influenza or a coronavirus disease.

This invention discloses a data-driven modeling and predictive control method for respiratory virus inactivation, relating to the field of biomedical technology. The method includes the following steps: obtaining initial viral characteristic data from a viral surface structure database through molecular dynamics simulation, processing the data to obtain a structural change matrix of the virus under different inactivation conditions; using a support vector machine classifier based on the structural change matrix to extract features of viral characteristics and antibody binding sites, determining a set of potential unexpected interaction sites. This data-driven modeling and predictive control method for respiratory virus inactivation effectively improves the scientific validity and stability of inactivation process parameters, reduces safety risks caused by improper inactivation conditions, shortens the process optimization cycle, reduces experimental costs, and helps maintain good immunogenicity while ensuring the safety of virus inactivation, thereby better meeting the practical application needs in vaccine development and virus prevention and control.

The application provides a method for regulating mechanical stretching force on SARS-CoV-2 pseudovirus invasion of alveolar epithelial cells. It comprises the following steps: screening SARS-CoV-2 pseudovirus infection titer capable of meeting infection efficiency; taking SARS-CoV-2 pseudovirus of the titer screened in step S1 to infect alveolar epithelial cells (ATII cells) in a biomimetic microfluidic alveolar cell chip, at the same time, applying gradient cyclic mechanical stretching force to the biomimetic microfluidic alveolar cell chip, monitoring the infection efficiency of SARS-CoV-2 pseudovirus by immunofluorescence staining, and establishing a relationship model between mechanical stretching force and infection efficiency; based on the relationship model between mechanical stretching force and infection efficiency, adjusting the mechanical stretching force, thereby adjusting the infection efficiency of SARS-CoV-2 pseudovirus in the biomimetic microfluidic alveolar cell chip. The application reveals that the stretching force can reduce the membrane tension of ATII cells, and further inhibit the infection efficiency of SARS-CoV-2 pseudovirus on ATII cells, thereby providing an important theoretical basis for elucidating the mechanical regulation mechanism of respiratory virus invasion.

The application relates to the technical field of respiratory virus prevention, and particularly relates to a protein complex and application of the protein complex in preparation of a composition for preventing and / or treating respiratory syncytial virus related epidemic diseases. The protein complex is composed of F protein (preF3-SpyT) containing a SpyTag label and a ferritin nanoparticle containing a SpyCatcher. The F protein and the ferritin nanoparticle are covalently connected in a high-efficiency and specific manner through optimization of a coupling process of the two, and finally form a target protein complex. The protein complex provided by the application has the characteristics of high purity, uniform particle size, excellent structure stability and significantly enhanced immunogenicity, can effectively stimulate specific immune responses of a body against respiratory syncytial virus (RSV), provides a brand-new technical solution for clinical transformation of an RSV vaccine, and has important clinical application and transformation values.

Abstract: This invention discloses a traditional Chinese medicine composition (Da Zhuque Decoction) with multi-target antiviral and anti-inflammatory activities, its preparation method, and its application. The composition consists of the following raw materials in parts by weight: Alisma plantago-aquatica 2-4 parts, Prunus armeniaca 4-6 parts, Chrysanthemum indicum 4-6 parts, Artemisia annua 5-7 parts, Morus alba leaf 5-7 parts, Gypsum 5-7 parts, Poria cocos 7-9 parts, Scutellaria baicalensis 8-10 parts, Rehmannia glutinosa 8-10 parts, Plantago asiatica 11-13 parts, Taraxacum mongolicum 14-16 parts, and Glycyrrhiza uralensis 3-5 parts. This invention overcomes the limitations of traditional qualitative formulation of traditional Chinese medicines. By limiting the specific synergistic ratio of artemisinin and baicalin (1:2.5~3.0), it achieves highly efficient inhibition of respiratory viruses (IC50=12.5μg / mL) and significant downregulation of inflammatory factors (IL-6, TNF-α). Meanwhile, the preparation process of "plaster decoction first - licorice extraction alone" solves the problem of easy decomposition of heat-sensitive components, increasing the artemisinin retention rate to over 95%. This invention has a clear material basis, controllable quality, and is suitable for preparing drugs or health products for the prevention and treatment of viral pneumonia.

The application discloses a microfluidic chip pathogen rapid detection reagent based on nano-antibody two-photon signal amplification and application thereof in rapid diagnosis of influenza A virus, influenza B virus and syncytial virus. The two-photon signal amplification system is pre-integrated in the microfluidic chip by adopting ultrasonic wave spraying modification PMMA base material and combining with piezoelectric ceramic nozzle precise spraying pL level droplets. During detection, UCNPs mark specific antibody I is combined with three virus target antigens, and then is combined with PLNPs mark specific antibody II on the chip to complete the multi-target synchronous quantitative detection by means of the fluorescence signal. The application overcomes the short board of traditional virus detection, can reach pg / ml level high-specificity detection, realizes rapid and accurate triple virus screening by means of microfluidic automatic operation, and provides a new type of efficient and feasible technical scheme for synchronous rapid detection of the three respiratory viruses.

This document discloses methods and compositions for use in various therapeutic, preventive, and control measures comprising from about 0.00001% by weight to about 100% by weight of an extract derived from sugarcane. The therapeutic, preventive, and control measures involve administering the extract to a subject, the extract comprising from about 10 catechin equivalents (CE) g / L to about 50 CE g / L of polyphenols or from about 100 CE mg / g to about 500 CE mg / g of polyphenols. These therapeutic and preventive measures include antimicrobial activity, particularly against respiratory viruses in humans, and treatment of microsporidia infections, such as EHP in shrimp and prawns. Control measures include reducing methane production in ruminants. The use of these compositions in, for example, dairy cows improves cow health by minimizing mastitis while increasing milk yield.

Methods are provided for inhibiting respiratory viruses (i.e., viruses associated with respiratory disease, such as influenza A, influenza B, RSV, etc.) in a sample. Embodiments of the methods include contacting a sample containing viral RNA (vRNA) bearing a target motif with an effective amount of an agent that specifically binds to the target motif and inhibits the respiratory virus. Also provided are methods for treating or preventing a respiratory viral infection in a subject. Also provided are compounds and pharmaceutical compositions comprising oligonucleotide sequences complementary to target vRNA regions used in the subject methods.

The application discloses a magnetic fluorescence immunochromatographic detection system and method for capturing virus nucleoprotein based on lectin, adopts magnetic fluorescence nano probes with surface modified wheat germ agglutinin as a general capture label, replaces traditional specific capture antibody label, combines with an immunochromatographic test strip pre-coated with specific antibodies, and constructs a non-paired heterologous sandwich detection mode of "lectin-virus nucleoprotein-specific antibody". The application discloses a mechanism that WGA is combined with specific conservative sites of SARS-CoV-2, influenza A virus and influenza B virus nucleoprotein through non-covalent bonds, and the combination sites are independent of the glycosyl recognition sites of WGA, so that WGA has the ability to resist high glycosylation protein interference. The method uses magnetic enrichment technology to remove matrix interference, combines high fluorescence intensity of quantum dots, and realizes super-sensitive detection of various respiratory virus nucleoproteins. The application is suitable for on-site rapid screening of complex clinical samples and environmental samples.