1115 results about "Microbacterium" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to recombinant escherichia coli containing a PDRN sequence and a preparation method thereof, and the preparation method comprises the following steps: S1, preparing a PDRN solution; s2, complementing the double strands of the PDRN into blunt ends; s3, constructing a recombinant plasmid; s4, carrying out mixed culture on a product obtained in the step S3 and escherichia coli DH5alpha; s5, screening the recombinants; s6, carrying out PCR (Polymerase Chain Reaction) amplification, and recording a strain from a fragment with the length of more than 200bp; s7, preparing a seed solution by adopting the strain prepared in the step S6; s8, strain fermentation; and S9, centrifugally collecting thalli. The method comprises the following steps: constructing a recombinant plasmid containing a PDRN fragment, introducing the recombinant plasmid into Escherichia coli, subsequently culturing the recombinant Escherichia coli to copy DNA in the Escherichia coli, extracting a purified exogenous gene fragment to obtain a strain containing a single fragment PDRN sequence, and getting rid of dependence on raw materials during subsequent extraction.

The invention relates to the technical field of microbial fermentation, and discloses a genetically engineered bacterium of high-yield D-pantothenic acid as well as a preparation method and application of the genetically engineered bacterium. The D-PA yield of the engineering bacteria is greatly improved through the following effects that acetolactate synthase IlvB and IlvN shunted by pyruvic acid are subjected to overexpression, meanwhile, a transcription attenuation region is deleted, and valine feedback inhibition sites are mutated; the method comprises the following steps: overexpressing keto-alcohol acid reductoisomerase IlvC and dihydroxy acid dehydratase IlvD, and converting the IlvC coenzyme preference from NADPH into NADH, so as to break through the upstream of D-PA synthesis; overexpression of PanB is carried out, overexpression of a serine-glycine transport system is carried out, and synthesis of 5, 10-methylenetetrahydrofolic acid is promoted; meanwhile, the expression of ketopantoic acid reductase PanE is enhanced; and finally, over-expressing pantothenic acid synthetase PanC, and increasing the content of a cofactor ATP required by the PanC. According to the engineering bacteria provided by the invention, through overall reinforcement of the multiple modules, cell growth is improved due to balance of cofactors and increase of D-PA, and the yield of D-PA reaches an unprecedented height.

The invention provides a bifidobacterium animalis subsp. Lactis strain B21247 with a hypoglycemic function and application thereof, and belongs to the technical field of functional microorganisms. The invention relates to a bifidobacterium animalis subsp. Lactis strain B21247 with a hypoglycemic function. The preservation number of the bifidobacterium animalis subsp. Lactis strain B21247 is GDMCC No: 65075. The strain B21247 has a good intestinal colonization function, and has the activity of inhibiting alpha-glucosidase in vitro. Meanwhile, a type 2 diabetes mouse model is used as an experimental model to verify that the strain B21247 achieves the effect of reducing blood sugar by adjusting glycosylated hemoglobin, reducing pancreatic beta cell damage and increasing secretion of intestinal GLP-1, and a new thought is provided for reducing blood sugar or treating diabetes.

The present invention relates to a recombinant yeast with high squalene production, a construction method and a use thereof, wherein the recombinant yeast is a saccharomyces cerevisiae that expresses or overexpresses HMGR gene, ADA gene, LEU2 gene, MVA pathway gene and squalene synthase gene ERG9. The present invention integrates acetaldehyde dehydrogenase gene ADA and HMG-CoA reductase gene tHMGR in multiple copies in saccharomyces cerevisiae, integrates the screened high-activity phosphoketolase gene PK and phosphotransacetylase gene PTA, weakens epoxysqualene synthesis, strengthens the mevalonate pathway, overexpresses yeast endogenous squalene synthase gene, etc. to obtain a recombinant yeast with high squalene production, so that the production in the shake flask stage of the production process exceeds 5g / L, and the production in the fed batch fermentation stage exceeds 50g / L, breaking through the current bottleneck of microbial synthesis of squalene production, and is suitable for practical promotion and application.

The invention relates to a saccharomyces cerevisiae strain for efficiently synthesizing cordycepin as well as a construction method and application of the saccharomyces cerevisiae strain, and belongs to the technical field of microbial genetic engineering. The construction method provided by the invention comprises the following steps: carrying out multi-copy genome integrated expression on heterologous codon optimized 2 '-carbonyl-3'-deoxyadenosine reductase genes and 3 '-adenosine monophosphate phosphohydrolase genes, so as to increase the dosage of key enzymes; and supplementing the defective gene of the engineering strain to obtain the saccharomyces cerevisiae strain B5U3 with high yield of cordycepin. According to the obtained saccharomyces cerevisiae strain for efficiently synthesizing the cordycepin, the cordycepin yield reaches 2.92 g / L after shake flask fermentation, the cordycepin yield reaches 7.5 g / L after fermentation in a fermentation tank, and a foundation is laid for safer and more reliable industrial production of the cordycepin.

The invention belongs to the field of agricultural biotechnology and environmental microbiological engineering, and discloses mesotrione nitroreductase Mstnr as well as a coding gene and application thereof. The nucleotide sequence of the gene is SEQ ID NO.1, the full length is 678bp, 225 amino acids are encoded, and the amino acid sequence of the gene is SEQ ID NO.2. The highest homology of the Mtnr and reported nitroreductase is only 49.2%, so that the Mtnr is a new nitroreductase gene. The nitroreductase Mtnr provided by the invention can catalyze nitro reduction of mesotrione and convert mesotrione into a product 2-amino-4-methylsulfonylbenzoic acid (AMBA) without herbicidal activity, which indicates that the Mtnr gene has a huge application prospect in biodegradation detoxification and repair of mesotrione.

The invention discloses bifidobacterium breve BBr69 with an immunoregulation function as well as application, a product and a method thereof, and belongs to the technical field of microorganisms. The preservation number of the bifidobacterium breve BBr69 with the immunoregulation function is CGMCC (China General Microbiological Culture Collection Center) NO. 32425. Based on the strain, the invention also provides application and related products of the strain. The BBr69 strain has broad-spectrum antibacterial performance, has the function of regulating intestinal flora and also has the function of enhancing immunity, and immunity is enhanced by proliferating RAW264.7 cells, increasing the content of cell factors, inhibiting weight loss, increasing the index of immune organs and reducing ileum tissue damage.

The invention discloses acinetobacter picoteri and application thereof, and relates to the field of microorganisms. The problem that an existing single saline-alkaline stress saline-alkaline tolerant strain cannot better adapt to the saline-alkaline environment of the Songnen plain to meet plant growth is solved. The collection number of the Acinetobacter pittii is CCTCC (China Center for Type Culture Collection) NO: M2024440, and the collection unit of the Acinetobacter pittii is the China Center for Type Culture Collection. The strain is classified as Acinetobacter sp. On the basis of physiological and biochemical characteristics and 16S rRNA (ribosomal Ribonucleic Acid) analysis. The acinetobacter picoteri can grow in an LB culture medium with pH of 8.5-12.0 and soda salt of 80-250 mmol / L, and has the capabilities of dissolving inorganic phosphorus, dissolving potassium and producing indoleacetic acid; the saline-alkali environment of the Songnen plain can be better adapted, and rice seed germination and seedling growth under saline-alkali stress are promoted.

The invention provides a saline-alkaline tolerant Bachu halomonas strain, which is characterized in that the saline-alkaline tolerant Bachu halomonas strain is named as halomonas sp. And is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.28831, and the preservation date is November 01, 2023; the nucleotide sequence of the 16S rRNA is as shown in SEQ ID NO: 1. The invention further provides application of the strain, and the strain is used for improving saline-alkaline resistance of plants, reducing soil salinity and alkalinity and promoting plant growth and is used for preparing fertilizer with plant growth promoting and saline-alkali soil improving effects. The strain disclosed by the invention has the functions of resisting salt and alkali, reducing salt and alkali, fixing nitrogen, decomposing organic phosphorus, dissolving inorganic phosphorus, producing IAA, producing oxidase, producing catalase, producing volatile acidic substances and promoting plant growth. The strain obtained by the invention has important application values in the aspects of improving saline-alkali stress resistance of crops, improving physicochemical properties of saline-alkali soil, increasing crop yield and the like, can be prepared into a fertilizer with plant growth promoting and saline-alkali soil improving effects for use, is a great innovation in saline-alkali soil improvement and crop planting, and has a wide application prospect.

The invention provides a keto-alcohol acid reductoisomerase mutant and application of the keto-alcohol acid reductoisomerase mutant in construction of genetically engineered bacteria for high yield of D-pantothenic acid. In order to solve the technical problem that in the prior art, the yield of D-pantothenic acid synthesized through microbial fermentation is not high, semi-rational design of enzyme is adopted, and a keto-alcohol acid reductoisomerase mutant with substrate specificity is obtained and applied to construction of genetically engineered bacteria for high yield of D-pantothenic acid. According to the invention, a genetically engineered bacterium with high yield of D-pantothenic acid is constructed by utilizing the keto-alcohol acid reductoisomerase mutant through a CRISPR-Cas9 gene editing technology, so that the yield of D-pantothenic acid is increased by about 12.04%, and compared with an original strain, the valine accumulation amount in obtained fermentation liquor is almost unchanged compared with that of a control, and the yield of D-pantothenic acid is greatly improved. However, the accumulation of branched chain amino acid isoleucine is less, so that the modification of the substrate specificity of the keto-alcohol acid reductoisomerase is achieved.

The present invention provides: a sludge decomposing bacterium having a 16S rRNA gene comprising a base sequence having 97% or more identity with the base sequence recorded in sequence number 1, a bacterium having a 16S rRNA gene comprising a base sequence having a base variation number of 2 bases or less relative to the base sequence recorded in sequence number 1 and having target microorganism decomposing ability, and a microbial preparation for decomposing a target microorganism comprising the bacterium (a1), wherein the bacterium (a1) is a bacterium having a 16S rRNA gene comprising a base sequence having 90% or more identity with the base sequence recorded in sequence number 1.

The invention relates to the technical field of synthetic biology and microbial fermentation, in particular to a 5-aminolevulinic acid synthetase mutant and application thereof. According to the invention, 5-aminolevulinic acid synthetase derived from rhodobacter capsulatus is expressed in recombinant Escherichia coli, point mutation is carried out on non-conservative amino acid of wild-type ALAS to obtain I86C mutants, the enzymatic activity of the 5-aminolevulinic acid synthetase is improved, and the ability of the 5-aminolevulinic acid synthetase in synthesis of 5-aminolevulinic acid is improved. The method can be used for solving the problems that the enzyme activity of the ALAS is low and the activity of the ALAS is easily inhibited by high-concentration substrate glycine and the like at present, and the yield of the 5-ALA is increased.

The invention relates to a marine-source microbacterium laminae CJB-Jou02 strain capable of efficiently biodegrading PE (polyethylene) microplastics, and the degradation capability of the marine-source microbacterium laminae CJB-Jou02 strain is evaluated. According to the microbacterium capable of degrading the PE micro-plastic leaves, the microbacterium is screened out from a marine mud sample, the preservation number of the strain is CGMCC 29643, the strain treats high-density PE micro-plastic for 30 days, the weight loss rate reaches up to 16.6 + / -0.46%, and the microbacterium can be used for degrading the PE micro-plastic leaves. Based on test results of a scanning electron microscope, a Fourier transform infrared spectrum, a water contact angle instrument and an X-ray diffractometer, the strain is proved to have the capability of efficiently biodegrading the PE micro-plastic, the compact crystal structure of the PE micro-plastic is damaged, the particle diameter is obviously reduced, and the hydrophilicity is increased. Meanwhile, the invention also finds that the microbacterium leaf strain CJB-Jou02 has certain degradation capacity on PE (polyethylene) membranes and microplastic bacteria such as PA (polyamide), PP (polypropylene), PS (polystyrene), PET (polyethylene terephthalate) and the like, and a new solution is provided for solving the problem of environmental pollution of PE microplastics.

The invention discloses a method for traceless gene editing of gram negative bacteria, a carrier and application of the carrier, and belongs to the technical field of microbial genetic engineering. The invention provides a vector for performing traceless gene editing on bordetella pertussis. The vector simultaneously comprises an antibiotic positive screening gene expression box and a sacB and galk negative screening gene expression box. According to the method, the sacB gene and the galk genome are synthesized into the double-reverse-screening system for the first time, and compared with a single-reverse-screening system, the reverse-screening efficiency is remarkably improved, and the dosage of a reverse-screening reagent is remarkably reduced. The invention provides a novel gene editing method for non-mode bacteria such as pertussis bordetella and the like, and has a good application prospect.

The invention provides an FWCH2 monoclonal antibody and a preparation method and application of a kit of the FWCH2 monoclonal antibody. A monoclonal antibody hybridoma cell strain FWCH2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.46112, and the prepared monoclonal antibody can effectively recognize the FWCH2.

The invention discloses an RAA-CRISPR / Cas12a detection system of a circle virus avian origin type 1 and application of the RAA-CRISPR / Cas12a detection system, and relates to the technical field of microbiological detection. The detection system comprises an RAA amplification primer pair, crRNA, an FQ-ssDNA probe, an FB-ssDNA probe and an LbCas12a nuclease, and is characterized in that the detection system comprises an RAA amplification primer pair; the RAA amplification primer pair comprises an upstream primer of which the nucleotide sequence is shown as SEQ ID NO.5 and a downstream primer of which the nucleotide sequence is shown as SEQ ID NO.8; the nucleotide sequence of the crRNA is as shown in SEQ ID NO. 11. The RAA-CRISPR / Cas12a detection kit established by adopting the detection system developed by the invention has the advantages of high efficiency, high specificity and high sensitivity, can be used for on-site rapid detection of GyVg1, and provides a powerful diagnostic tool with a cost advantage for a basic-level working environment.

The invention belongs to the field of agricultural biotechnology and environmental microbiological engineering, and discloses a novel mesotrione nitroreductase Bamnr, and a coding gene and application thereof. The nucleotide sequence of the gene is SEQ ID NO.1, the full length is 639bp, 212 amino acids are encoded, and the amino acid sequence of the gene is SEQ ID NO.2. The highest homology of Bamnr and reported nitroreductase is only 69.4%, so that Bamnr is a new nitroreductase gene. The nitroreductase Bamnr provided by the invention can catalyze nitro reduction of mesotrione, and the mesotrione is converted into a product 2-amino-4-methylsulfonyl benzoic acid (AMBA) without herbicidal activity, so that the Bamnr gene has a huge application prospect in biodegradation detoxification and repair of the mesotrione.

The invention belongs to the field of agricultural biotechnology and environmental microbiological engineering, and discloses a herbicide mesotrione degradation detoxification enzyme protein, and a coding gene and application thereof. The nucleotide sequence of the gene is SEQ ID NO.1, the full length of the gene is 738bp, 245 amino acids are encoded, and the amino acid sequence of the gene is SEQ ID NO.2. The maximum homology of the Mdmnr and reported nitroreductase is only 48%, so that the Mdmnr is a new nitroreductase gene. The nitroreductase Mdmnr provided by the invention can catalyze nitro reduction of mesotrione, and the mesotrione is converted into a product AMBA without herbicidal activity, which indicates that the Mdmnr gene has huge application prospects in mesotrione-resistant transgenic engineering and biodegradation detoxification and repair of mesotrione residual pollution.

The invention provides a ZN428 monoclonal antibody and a preparation method and application of a kit of the ZN428 monoclonal antibody. The monoclonal antibody hybridoma cell strain ZN428 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.46111, and the prepared monoclonal antibody can effectively recognize the ZN428.

The invention provides an LRC58 monoclonal antibody and a preparation method and application of a kit of the LRC58 monoclonal antibody. The monoclonal antibody hybridoma cell strain LRC58 is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.46113, and the prepared monoclonal antibody can effectively recognize the LRC58. The invention further discloses a method for preparing the monoclonal antibody.

The invention relates to the technical field of microbial metabolism engineering, in particular to a construction method and application of genetically engineered bacteria for efficiently producing O-acetyl-L-homoserine. The construction method specifically comprises the following steps: (1) taking a strain Escherichia coli W3110 as a chassis, and knocking out a homoserine O-succinyl transferase coding gene metA; (2) knocking out a gamma-cystathionine synthetase encoding gene metB; (3) knocking out a cysteine lyase coding gene metC; (4) carrying out weakening expression on an O-acetyl-L-homoserine thiolase coding gene metYcm from the marine cyclobacterium; (5) overexpressing a ribulose diphosphate carboxylase coding gene rbcLSse from the synechococcus elongate; (6) overexpressing a ribulose phosphate kinase coding gene prkse from the synechococcus slender; and (7) weakening the expression of the citrate synthase coding gene gltA to obtain the genetically engineered bacterium for efficiently producing the O-acetyl-L-homoserine. The strain provided by the invention is used for producing O-acetyl-L-homoserine, and has the advantages of high yield, high yield and high sugar-acid conversion rate.

The present disclosure is directed to an engineered lysin comprising: (a) a Gram-negative base lysin; and (b) one or more antimicrobial peptide (AMP) and / or cell penetrating peptide (CPP), and optionally an outer membrane binding motif and at least one linker, or an active fragment thereof, or a variant thereof having at least 80% sequence identity. Also disclosed herein are methods of treating a bacterial infection caused by at least one Gram-negative bacteria and methods of killing at least one Gram-negative bacteria, comprising administering the engineered lysin disclosed herein.

The invention relates to an application of a cytochrome P450 reductase 10273 inactivating agent in construction of an aspergillus terreus engineering strain for high yield of emodin or a derivative thereof. The invention further relates to aspergillus terreus engineering bacteria for high yield of emodin or the emodin derivatives and a construction method of the aspergillus terreus engineering bacteria. By knocking out the cytochrome P450 reductase gene 10273, the activation of anthraquinone methyl hydroxylase 4804 is blocked in aspergillus terreus, so that the hydroxylation pathway of the emodin is blocked, the purity of the emodin and the emodin-3-methyl ether in aspergillus terreus cells is improved, the production cost of the emodin and the emodin-3-methyl ether is reduced, and the method is suitable for industrial production. And a foundation is provided for the microbial industrial synthesis of the emodin and the emodin-3-methyl ether.

The invention relates to a recombinant bacterium for overexpressing uranyl binding protein and application of the recombinant bacterium, and belongs to the technical field of microorganisms. Bacterial outer membrane protein and uranyl binding protein are expressed in host bacteria, and the uranyl binding protein is anchored on an outer membrane of recombinant bacteria through the bacterial outer membrane protein, so that the uranyl binding protein anchored on the bacterial outer membrane can adsorb uranyl ions. Meanwhile, the invention finds that the expression quantity of the uranyl binding protein can be increased by performing fusion expression on the uranyl binding protein by using LPP-OMPA, so that the adsorption effect of uranyl ions is improved. The recombinant bacterium is prepared into a freeze-dried capsule product, and the freeze-dried capsule product can penetrate through a stomach barrier and is accurately delivered to intestinal tracts so as to realize uranyl ion adsorption.

Provided herein are compositions and methods for inducing an immune response in a subject, wherein the compositions comprise apoptotic cells, extracellular vesicles (EVs), and combinations thereof. Various compositions comprising mRNA encoding a microbial antigen are also provided. Further provided herein are compositions engineered to overexpress the human arrestin domain containing protein 1 [ARRDC1].

The invention provides a keto-alcohol acid reductoisomerase mutant and application of the keto-alcohol acid reductoisomerase mutant in construction of genetically engineered bacteria for high yield of D-pantothenic acid. In order to solve the technical problem that in the prior art, the yield of D-pantothenic acid synthesized through microbial fermentation is not high, semi-rational design of enzyme is adopted, and a keto-alcohol acid reductoisomerase mutant with substrate specificity is obtained and applied to construction of genetically engineered bacteria for high yield of D-pantothenic acid. According to the invention, a genetically engineered bacterium with high yield of D-pantothenic acid is constructed by utilizing the keto-alcohol acid reductoisomerase mutant through a CRISPR-Cas9 gene editing technology, so that the yield of D-pantothenic acid is increased by about 12.04%, and compared with an original strain, the valine accumulation amount in obtained fermentation liquor is almost unchanged compared with that of a control, and the yield of D-pantothenic acid is greatly improved. However, the accumulation of branched chain amino acid isoleucine is less, so that the modification of the substrate specificity of the keto-alcohol acid reductoisomerase is achieved.

The present disclosure is directed to an engineered lysin comprising: (a) a Gram-negative base lysin; and (b) one or more antimicrobial peptide (AMP) and / or cell penetrating peptide (CPP), and optionally an outer membrane binding motif and at least one linker, or an active fragment thereof, or a variant thereof having at least 80% sequence identity. Also disclosed herein are methods of treating a bacterial infection caused by at least one Gram-negative bacteria and methods of killing at least one Gram-negative bacteria, comprising administering the engineered lysin disclosed herein.

The invention discloses an ensifer sp.NJF-2 capable of performing biological defluorination on a polyfluoroalkyl compound, the ensifer sp.NJF-2 is preserved in China General Microbiological Culture Collection Center on September 12, 2024, and the preservation number is CGMCC NO.1. 19397. The invention also discloses an application of the strain in the aspect of biological defluorination, in-vivo and in-vitro transformation experiments show that the ensifer sp.NJF-2 can perform biological defluorination on the polyfluoroalkyl compound, and the ensifer sp.NJF-2 can perform biological defluorination on the polyfluoroalkyl compound. The strain can achieve the biological defluorination effect on various PFAS such as monofluorine, trifluorine, hexafluorine and the like.

The invention provides a CK052 monoclonal antibody and a preparation method and application of a kit of the CK052 monoclonal antibody. The monoclonal antibody hybridoma cell strain CK052 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.46107. The prepared monoclonal antibody and the kit thereof can effectively recognize the CK052.

The invention discloses lactobacillus mucus HG002 and application thereof, and belongs to the field of microbial medicine. The lactobacillus mucilaginosus HG002 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.36670, and the 16S rDNA gene sequence of the lactobacillus mucilaginosus HG002 is as shown in SEQ ID NO: 1. The strain is separated from excrement of healthy adults, the relieving effect of ALF is remarkably improved through the brand-new fermentation lactobacillus mucus HG002 strain and derivative EVs thereof in combination with a synergistic effect mechanism and optimized product design, many defects in the prior art are overcome, and wide application prospects and industrialization value are achieved.