190 results about "Clostridium bacteria" patented technology

The invention discloses clostridium and application thereof in fermentation production of alcohol, and belongs to the technical field of industrial microorganisms. The clostridium is a carbon monoxide eating clostridium strain GG6, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.34975, and the preservation time is June 23, 2025. The clostridium is a carbon monoxide eating clostridium strain GG6 which is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.34975, and the preservation number is CGMCC No.34975. The carbon monoxide eating clostridium GG6 is obtained by enriching tail gas flue sediments of a coal chemical plant through an abundant culture medium, evolving a screening culture medium and sorting single bacteria, the bacterial strain is fermented by taking synthesis gas (CO / H2 / CO2) as raw material gas to generate acetic acid, ethanol, butyric acid and butanol, the thallus biomass OD600 is more than or equal to 6, the concentration of produced ethanol is more than or equal to 10g / L, the concentration of produced butanol is more than or equal to 2.5 g / L, and the concentration of produced butanol is more than or equal to 2.5 g / L; and commercialized implementation of butanol production through gas fermentation is facilitated.

The invention provides a clostridium endophyllum engineering bacterium as well as a construction method and application thereof, and belongs to the technical field of biology, compared with a wild type strain, the clostridium endophyllum engineering bacterium can only produce acetic acid in a low-pH environment under the condition of taking CO2 and H2 as raw materials, and does not generate by-products such as ethanol, lactic acid, 2, 3-butanediol and the like. Besides, the fermentation liquor of the clostridium endophyllum engineering bacteria can be directly used as an acidic substrate to adjust the pH value of downstream yeast fermentation liquor, meanwhile, automatic addition of a carbon source is achieved, zero addition of inorganic salt in the cascade conversion process from CO2 and H2 to grease or yeast protein is achieved, and the economical efficiency and environmental friendliness of the whole process are improved.

The invention discloses a preparation method of bacterial powder of clostridium butyricum, and belongs to the technical field of bacterial powder preparation. The preparation method of the bacterial powder provided by the invention comprises the following steps: adding a regulator for regulating culture, heating, and adding a protective agent for freeze drying. Experimental results show that the preparation method of the bacterial powder provided by the invention has high viable bacterium yield, and the loss of clostridium butyricum in the preparation process of the bacterial powder is reduced; the bacterial powder obtained according to the bacterial powder preparation method still has the retention rate of 90% or above after being stored for a long time for 180 days at the temperature of 35 DEG C, which indicates that the survival ability of clostridium butyricum is remarkably improved. The preparation method of the bacterial powder is simple in process, easy to operate and environmentally friendly, and the cost cannot be remarkably increased. The clostridium butyricum microecological product provides a new strategy for preparation and activity maintenance of clostridium butyricum microecological products, can be applied to the fields of animal husbandry industry and medical care, and has a wide application prospect.

The invention discloses a compound micro-ecological preparation for aquatic products as well as a preparation method and application thereof, and belongs to the technical field of micro-ecological preparations. The microecological preparation disclosed by the invention comprises clostridium butyricum, bacillus licheniformis, enterococcus faecalis and lactobacillus plantarum. The microecological preparation is combined with concentrated cottonseed protein for use, can replace fish meal, is added into feed, can remarkably improve the immunocompetence, oxidation resistance and digestive enzyme activity of animals, and can improve the relative abundance of beneficial bacteria in intestinal tracts, inhibit the growth of pathogenic bacteria and regulate the microecological balance of the intestinal tracts after entering the intestinal tracts of the animals. According to the invention, aerobic bacteria and anaerobic bacteria are cooperatively used, oxygen in the surrounding environment is greatly consumed by bacillus licheniformis and enterococcus faecalis, the proliferation of beneficial bacteria such as clostridium butyricum and lactobacillus plantarum is promoted, and the probiotic effect is fully played.

The invention discloses a method for improving the performance of clostridium aerovorans for synthesizing ethanol by utilizing gas fermentation. The method comprises the following steps: enabling the clostridium aerovorans to over-express ethanol synthesis pathway genes ald, adhE1 and adhE2; the expression of genes CLJU23220, CLJU25810 and CLJU25840 in the synthetic route of the 2, 3-butanediol is inhibited; and overexpressing acetic acid reuse genes aor1 and aor2. According to the clostridium yangdall engineering bacteria constructed by the method, the yield of ethanol synthesized by fermentation of synthesis gas is increased by 700% or above and reaches 1.70 g / L or above, and the synthesis amount of a by-product acetic acid is reduced by 18%.

The invention belongs to the technical field of biological fermentation hydrogen production. The invention provides a method for producing hydrogen by dark fermentation of pericarp biomass, which comprises the following steps: drying pericarp, crushing, and sieving to obtain powdery pericarp particles; performing acid heat pretreatment on the powdery fruit peel particles to obtain fruit peel pretreatment liquid; and inoculating the clostridium butyricum solution into the pericarp pretreatment liquid, and carrying out dark fermentation under an anaerobic condition to obtain a fermentation gas containing hydrogen. According to the method, the pericarp byproducts are used for dark fermentation for hydrogen production, so that high-value recycling utilization of agricultural wastes is realized; lignin in the pericarp is effectively removed through acid heat pretreatment, the availability of cellulose and hemicellulose is remarkably improved, conditions such as acid concentration, temperature and time are optimized, the inhibition effect of by-products is avoided, and the hydrogen production efficiency is remarkably improved; moreover, the method has the advantages of good stability and high repeatability, and provides a new technical approach for efficient production of clean energy.

The invention discloses a prebiotic composition capable of proliferating intestinal beneficial flora and a preparation method thereof, and the prebiotic composition comprises the following raw materials in parts by weight: 35-45 parts of partially hydrolyzed complex gum, 30-40 parts of green banana resistant starch, 15-25 parts of enzymolysis okra polysaccharide and 3-5 parts of tea polyphenol. According to the composition, the partially hydrolyzed complex gum with specific molecular weight, the green banana resistant starch, the enzymolysis okra polysaccharide and the tea polyphenol are scientifically compounded, so that efficient proliferation of intestinal effective microbial communities and comprehensive and positive regulation and control of intestinal microenvironment are realized, and the composition is prepared by utilizing four components with complementary functions and different effects. The bacillus amyloliquefaciens can effectively proliferate traditional beneficial bacteria such as bifidobacteria and lactobacillus, can effectively nourish butyric acid producing bacteria such as clostridium praeparatum and inhibit growth of harmful bacteria, so that the bacillus amyloliquefaciens has the function of remarkably improving intestinal flora disorder caused by ampicillin, and has the functions of promoting generation of short-chain fatty acid and maintaining intestinal barriers.

The invention discloses a method for rapidly enriching pit mud high-yield caproic acid functional flora and application, and belongs to the technical field of baijiu brewing microorganisms. According to the method, the aged pit mud which is screened through tests and has better quality is used as seed mud, and the selected high-quality pit mud is subjected to step-by-step amplification culture by adopting a specific culture medium. The obtained bacterial community is a caproic acid producing bacterial community (85%) with the caproic acid producing clostridium as the main body, and the bacterial community can produce more than 10 g / L of caproic acid and less than 2 g / L of butyric acid. The mixed flora obtained by the method disclosed by the invention can be used for culturing and repairing the pit mud, increasing the caproic acid-producing main flora in the pit mud, promoting the pit mud to be aged, preventing the pit mud from being aged and hardened, and effectively improving the quality of raw wine at the same time. And the mixed flora can also be used for fermenting the byproduct yellow water of the Luzhou-flavor liquor to produce caproic acid at high yield, and can be further used for producing high-quality flavoring liquor.

Disclosed herein are methods for administering a dose of a microbiota recovery therapy composition by colonoscopy. The methods disclosed herein are useful for the treatment of diseases and / or conditions of the digestive tract, such as Clostridium difficile infection (GDI), including recurrent GDI, and other gastrointestinal disorders. The method can result in improved quality of life. These methods are capable of altering the gut microbiota composition and / or the gut metabolome composition. The microbiota recovery therapy composition comprises a fecal microbiota suspension comprising: a fecal microorganism derived from a human fecal sample, and a solution of polyethylene glycol and brine.

The invention provides a breeding method suitable for breeding litopenaeus vannamei in saline-alkali soil, and relates to the technical field of aquaculture. According to the breeding method suitable for breeding the litopenaeus vannamei in the saline-alkali soil, the proportion of Ca < 2 + > to Mg < 2 + > to K < + > in a water body is optimized to be 2.8: 1: 0.3 by customizing a compound ion regulation and control solution, SO4 < 2-> / Cl <-> is controlled to be 0.14-0.8, and the pH is stabilized to be 8.2-8.8 in combination with a citric acid-sodium citrate buffer system; a slow-medium-fast segmented domestication strategy is adopted to adapt to different development stages of the fries; ethanol clostridium protein (CAP) is added into the feed to be combined with compound probiotics, so that immune metabolism regulation and control are enhanced. Experiments prove that according to the method, the survival rate of the litopenaeus vannamei seedlings reaches 86.3% + / -2.1%, the culture period is shortened by 12-15 days, the hepatopancreas SOD activity is improved by 42.6%, the vibrio inhibition rate reaches 78.5%, and the method is remarkably superior to the prior art. The method does not need large-scale pond transformation, adapts to different types of saline-alkali soil water bodies, realizes efficient green culture, and has important application value.

The invention discloses selenium functionalized clostridium butyricum-bacillus subtilis composite probiotic freeze-dried powder as well as a preparation method and application thereof. The compound probiotic freeze-dried powder is prepared from the following components in parts by mass: 20 to 30 parts of compound probiotic bacterial sludge, 10 to 20 parts of trehalose, 20 to 30 parts of fructo-oligosaccharide, 20 to 30 parts of dried skim milk, 8 to 10 parts of sodium alginate and 1 to 2 parts of calcium chloride. The preparation method comprises the following steps: blending selenium-functionalized clostridium butyricum, bacillus subtilis and a freeze-drying protective agent in sodium alginate hydrogel, and further performing vacuum freeze-drying to obtain the composite probiotic freeze-dried powder. The freeze-dried powder prepared by the invention continuously releases hydrogen through metabolism of probiotics, shows excellent active oxygen scavenging ability, and shows outstanding application value in the field of intestinal injury treatment. The method disclosed by the invention is simple and efficient in process and has good potential of large-scale production.

The invention relates to a method for producing a water environment protective agent by fermenting hermetia illucens larva slurry, and belongs to the technical field of microbial fermentation. According to the present invention, the hermetia illucens larvae are adopted as the main raw material, the main raw material is ground into the slurry through the colloid mill, the small amount of the carbon source is supplemented, the Phaffia rhodozyma CGMCC NO.29217, the Bacillus coagulans CGMCC NO.5233 and the Clostridium butyricum CGMCC NO.4729 are subjected to the mixed bacteria synergistic fermentation, and the produced product can be widely used in the aquaculture and other application scenarios. Fermented hermetia illucens larva serous fluid is rich in astaxanthin, a fermented product has obvious acid fragrance and no peculiar smell, the total number of probiotics is larger than or equal to 5.0 * 10 < 8 > cfu / g, the pH value is smaller than 4.50, and the content of small molecule active polypeptide (acid soluble protein) of the fermented product is increased by 2.5-3 times compared with that before fermentation. Bacillus subtilis CGMCC (B) 63501 is used as indicator bacteria to detect that the content of bacteriostatic active substances after fermentation of hermetia illucens larva serous fluid is larger than 1.0 g / kg, pathogenic bacteria such as salmonella, vibrio parahaemolyticus and vibrio cholerae are not detected, and normal-temperature storage can be achieved. The preparation method is low in production cost, easy to operate, safe and free of residues, and the economic benefits of aquaculture can be improved.

This invention belongs to the field of bioengineering and carbon one-carbon resource utilization technology, specifically relating to a bacterial strain that produces acetic acid and acetate through syngas fermentation, as well as its applications and methods. The strain is *Clostridium yongdarii* (…). Clostridium ljungdahlii SL40, deposited at the Guangdong Provincial Microbial Culture Collection Center (GDMCC No. 67679) on January 15, 2026, represents a strain that achieves highly efficient H2 / CO2 conversion in syngas, with a maximum CO2 utilization rate of 1.8 g / L / h. The anaerobic fermentation process produces zero pollutants, enabling resource utilization and carbon reduction of industrial waste gas, and providing a new technological pathway for carbon resource conversion.

The application provides a preparation of a fermentation bed and application of waste of the fermentation bed in preparation of special organic fertilizer for rice, belongs to the technical field of livestock breeding, and specifically relates to a fermentation bed for cattle breeding, which comprises raw materials: rice straw, rice husk, quicklime, corn flour, wheat bran, sawdust and mixed bacteria liquid A; the mixed bacteria liquid A comprises Bacillus subtilis, Clostridium butyricum, Rhodopseudomonas palustris, Saccharomyces cerevisiae and Lactobacillus acidophilus; and a special organic fertilizer for rice, which comprises components: the above-mentioned waste fermentation bed, rice straw, rice husk, wood ash, mixed bacteria liquid B and mixed enzyme preparation; the provided fermentation bed can effectively degrade cattle manure, solve the problem of dirty and smelly breeding environment, and effectively reduce the incidence of diseases such as cattle diarrhea; and the provided special organic fertilizer for rice meets the nutritional requirements of rice planting, improves the yield and stress resistance of rice, and prevents or reduces the occurrence of diseases.

The invention discloses a compound microbial agent and application thereof, and belongs to the technical field of microbial agents. The method specifically comprises the following steps: adding clostridium butyricum fermentation centrifugate into a fermentation tank, adding a nitrogen source, a carbon source and inorganic salt into the fermentation tank, then adding a bacillus subtilis solution and a bacillus licheniformis solution, stirring at a certain temperature and a certain rotating speed, fermenting for a period of time, then adding a lactic acid bacteria solution, and fermenting for a period of time at a certain rotating speed; stirring and fermenting at a certain rotating speed at a certain temperature, detecting the pH value after fermenting for a period of time, and when the pH value is reduced to 4.3-4.5, obtaining the compound microbial agent. The prepared compound microbial agent can improve the saline-alkali soil, effectively improve the pH of the saline-alkali soil, consume soil salinity and improve the crop germination rate.

The present invention relates to the genetic modification of a bacterium of the genus Clostridium, generally a solvent-producing bacterium of the genus Clostridium, in order to allow the production of propan-2-ol or a mixture comprising propan-2-ol and ethanol by said bacterium. Furthermore, methods, tools and kits for obtaining said bacteria, the genetically modified bacteria obtained and uses thereof are described.

The invention relates to the technical field of microorganisms, in particular to mixed bacteria fermentation liquor as well as a preparation method and application thereof. Bacterial strains in the mixed fermentation liquor comprise clostridium butyricum, enterococcus faecium and bacillus. The preparation method of the mixed bacteria fermentation liquor comprises the following steps: culturing the strains in the mixed bacteria fermentation liquor in a mixed bacteria fermentation culture medium to obtain the mixed bacteria fermentation liquor; wherein the bacterial strains comprise clostridium butyricum, enterococcus faecium and bacillus. According to the mixed bacterium fermentation liquor and the preparation method and application thereof, coexisting culture of donkey source enterococcus faecium, donkey source bacillus and donkey source clostridium butyricum can be achieved through the mixed bacterium fermentation liquor, and meanwhile the mixed bacterium fermentation liquor has the excellent effects of producing compound enzyme and inhibiting pathogenic bacteria and can serve as a feed additive to improve the reproductive performance of a female donkey and improve the growth performance of a donkey foal; the occurrence of donkey foal diarrhea is reduced, so that the economic benefit of donkey breeding is increased.

The present application belongs to the technical field of bacterial detection, and particularly relates to a Clostridium difficile multi-locus genotyping detection kit and application thereof. The present application aims to provide a new method to directly type a main Clostridium difficile strain sequence type (ST). The technical scheme of the present application is a multiplex fluorescence PCR primer set and probe for Clostridium difficile multi-locus genotyping detection, wherein the primer and probe are shown in Seq ID No. 1-18. The present application discloses a multiplex fluorescence PCR reagent for 7 main ST types of Clostridium difficile based on a fluorescence PCR-dissolution curve method. The reagent can simultaneously detect 7 main ST types of Clostridium difficile in the same PCR reaction system.

The present invention is a method for producing a fermentation broth containing colloidal particles having a particle size of not more than 50 nm and short-chain fatty acids, with an acidity of pH 3 to 4. The method is a method comprising the steps of: a step of preparing a fermentation device comprising a plurality of sets of fermentation vessels subjected to temperature management in each stage of a multi-stage fermentation process; a step of using soft water as a starter culture, a spore-forming Clostridium genus-containing primary culture solution containing 7 kinds of fermentation bacteria (International Depositary Accession Nos. NITE BP-02945 to NITE BP-02951) managed at a low temperature, and 3 kinds of fermentation media derived from natural raw materials generated by separately fermenting a first culture medium of dried soybeans, a second culture medium of a mixed culture medium of dried plants containing Chinese jujube, Chinese wolfberry fruit, and turmeric, and a third culture medium of a honey raw material; and a step of producing a fermentation broth by a multi-stage fermentation process.

The present application provides the use of a nanocomposite with a core-shell structure. Specifically, the present application provides the use of a nanocomposite with a core-shell structure in the preparation of a drug for preventing and / or treating Clostridium difficile infection and recurrent diseases caused thereby, wherein the nanocomposite comprises: ZnO nanoparticles as a core; mesoporous silica as a shell coated on the surface of the ZnO nanoparticles; an antibiotic loaded in the pore channel of the mesoporous silica; hyperbranched polylysine modified on the surface of the mesoporous silica; and a pH-responsive coating material as the outermost layer. The nanocomposite provided by the present application can achieve precise targeted release of the drug in the colon, and through the sequential and synergistic mechanism of "membrane breaking-sterilization-spore inhibition-repair", it can simultaneously solve multiple factors leading to CDI recurrence such as biofilm protection, spore storage, bacterial population destruction, mucosal damage, etc., and improve the treatment effect.

Provided is a carbonate-containing granular material production method with which it is possible to produce a carbonate-containing granular material at a cost lower than when using conventional methods.　The present invention has a carbonation step for bringing, into contact with a carbonation-target granular material at a maximum temperature of higher than 0°C to lower than 100°C, either water, a carbonation accelerator, and at least one among a carbon dioxide-containing substance, a carbonate ion-containing water, and a hydrogen carbonate ion-containing water, or a carbonation accelerator and at least one among a carbon dioxide-containing water, a carbonate ion-containing water, and a hydrogen carbonate ion-containing water. The total amount of water brought into contact in the carbonation step is 1 mass% or more of the carbonation-target granular material. The carbonation accelerator contains bacteria. The bacteria are at least one selected from the genus Bacillus, the family Bacillaceae, the class Bacilli, the family Clostridiaceae, the family Micrococcaceae, the family Microbacteriaceae, or the family Comamonadaceae.

A biological control method for centipede breeding belongs to the field of special economic animal breeding technology. This invention uses a compound probiotic strain of *Trichoderma longicornis*, *Streptomyces flavus*, *Bacillus subtilis*, *Bacillus licheniformis*, *Clostridium butyricum*, and lactic acid bacteria, which is fully incorporated into the breeding substrate. After 24-72 hours of static setting, the bacteria fully colonize and form a stable antibacterial state. The bacterial community can remain dormant during periods of nutrient deficiency, low-temperature overwintering, or year-end static setting, and will revive upon contact with organic matter. The method is supplemented with springtails and pink woodlice, which preferentially consume harmful bacteria, clean up uneaten feed, and primarily feed on and decompose centipede feces, forming a mutually beneficial symbiotic relationship with the biocontrol bacteria. Their feces further nourish the bacterial community, extending the stable effective period of the bacterial community from 2 months to 4-6 months or more. Simultaneously, probiotics and vitamins are added to the feed to strengthen the centipede's constitution. This invention constructs a three-dimensional synergistic prevention and control system that integrates matrix inhibition of pathogenic bacteria, environmental residue removal, and internal administration for enhanced protection. This system enables drug-free ecological disease control, significantly reduces the incidence of diseases such as green muscardine, and improves the survival rate of centipede farming. It is suitable for large-scale centipede farming.

The invention relates to a harmless treatment method for solid waste. The compound probiotic microbial agent comprises bacillus licheniformis, lactobacillus rimosii, lactobacillus plantarum, clostridium butyricum and saccharomycetes, the effective viable count is larger than or equal to 0.5 billion / mL, pig manure, corn straw and saw dust are mixed according to the mass ratio to obtain a compost raw material, the initial carbon-nitrogen ratio (C / N) is adjusted to be 25-35, the water content is adjusted to be 55-65%, and the compost raw material is fermented to obtain the compound probiotic microbial agent. And adding a composite probiotic inoculant accounting for 0.5-2.5% of the total mass into the compost raw material. Through probiotic combination and concentration optimization, the problems of decomposition and pollution of various ARGs are solved at the same time.

The invention relates to a novel bacillus subtilis (B.subtilis) with a strong inhibition effect on clostridium perfringens (C. perfringens), and an application of the bacillus subtilis as a probiotic.

The invention relates to bacillus velezensis Bv-GD2 and application of the bacillus velezensis Bv-GD2. The classification name of the bacillus velezensis Bv-GD2 is bacillus velezensis, and the preservation number of the bacillus velezensis Bv-GD2 is GDMCC No: 66722. The strain is wide in antibacterial spectrum and high in tolerance, has the enzyme production capacity and the antibacterial active substance production capacity, and can remarkably inhibit common intestinal pathogens such as clostridium perfringens, salmonella, staphylococcus aureus and escherichia coli, relieve intestinal lesions, improve the health degree of the intestinal environment and improve the growth performance of livestock and poultry.

The present application relates to a kind of genetically engineered bacteria of GLP-1 secretion and its preparation method and application, the genetically engineered bacteria CB-pMTL007-GLP-1 is prepared by integrating GLP-1 gene sequence into the genome of Clostridium butyricum NCU-02 (CGMCC No.25504), the genetically engineered bacteria has the effect of treating type 2 diabetes, reducing high blood pressure and improving Parkinson's disease.The present application uses Clostridium butyricum NCU-02 (CGMCC No.25504) as the host of recombinant expression vector for expressing GLP-1, so that the recombinant plasmid in the protein expression system of the GLP-1 secretion has good genetic stability, also has the same acid resistance, bile salt resistance, antioxidant capacity as wild-type Clostridium butyricum NCU-02 (CGMCC No.25504).