92 results about "Clostridium bacteria" patented technology

The invention discloses a method for improving the performance of clostridium aerovorans for synthesizing ethanol by utilizing gas fermentation. The method comprises the following steps: enabling the clostridium aerovorans to over-express ethanol synthesis pathway genes ald, adhE1 and adhE2; the expression of genes CLJU23220, CLJU25810 and CLJU25840 in the synthetic route of the 2, 3-butanediol is inhibited; and overexpressing acetic acid reuse genes aor1 and aor2. According to the clostridium yangdall engineering bacteria constructed by the method, the yield of ethanol synthesized by fermentation of synthesis gas is increased by 700% or above and reaches 1.70 g / L or above, and the synthesis amount of a by-product acetic acid is reduced by 18%.

Disclosed herein are methods for administering a dose of a microbiota recovery therapy composition by colonoscopy. The methods disclosed herein are useful for the treatment of diseases and / or conditions of the digestive tract, such as Clostridium difficile infection (GDI), including recurrent GDI, and other gastrointestinal disorders. The method can result in improved quality of life. These methods are capable of altering the gut microbiota composition and / or the gut metabolome composition. The microbiota recovery therapy composition comprises a fecal microbiota suspension comprising: a fecal microorganism derived from a human fecal sample, and a solution of polyethylene glycol and brine.

The invention provides a breeding method suitable for breeding litopenaeus vannamei in saline-alkali soil, and relates to the technical field of aquaculture. According to the breeding method suitable for breeding the litopenaeus vannamei in the saline-alkali soil, the proportion of Ca < 2 + > to Mg < 2 + > to K < + > in a water body is optimized to be 2.8: 1: 0.3 by customizing a compound ion regulation and control solution, SO4 < 2-> / Cl <-> is controlled to be 0.14-0.8, and the pH is stabilized to be 8.2-8.8 in combination with a citric acid-sodium citrate buffer system; a slow-medium-fast segmented domestication strategy is adopted to adapt to different development stages of the fries; ethanol clostridium protein (CAP) is added into the feed to be combined with compound probiotics, so that immune metabolism regulation and control are enhanced. Experiments prove that according to the method, the survival rate of the litopenaeus vannamei seedlings reaches 86.3% + / -2.1%, the culture period is shortened by 12-15 days, the hepatopancreas SOD activity is improved by 42.6%, the vibrio inhibition rate reaches 78.5%, and the method is remarkably superior to the prior art. The method does not need large-scale pond transformation, adapts to different types of saline-alkali soil water bodies, realizes efficient green culture, and has important application value.

The invention discloses selenium functionalized clostridium butyricum-bacillus subtilis composite probiotic freeze-dried powder as well as a preparation method and application thereof. The compound probiotic freeze-dried powder is prepared from the following components in parts by mass: 20 to 30 parts of compound probiotic bacterial sludge, 10 to 20 parts of trehalose, 20 to 30 parts of fructo-oligosaccharide, 20 to 30 parts of dried skim milk, 8 to 10 parts of sodium alginate and 1 to 2 parts of calcium chloride. The preparation method comprises the following steps: blending selenium-functionalized clostridium butyricum, bacillus subtilis and a freeze-drying protective agent in sodium alginate hydrogel, and further performing vacuum freeze-drying to obtain the composite probiotic freeze-dried powder. The freeze-dried powder prepared by the invention continuously releases hydrogen through metabolism of probiotics, shows excellent active oxygen scavenging ability, and shows outstanding application value in the field of intestinal injury treatment. The method disclosed by the invention is simple and efficient in process and has good potential of large-scale production.

The invention relates to a method for producing a water environment protective agent by fermenting hermetia illucens larva slurry, and belongs to the technical field of microbial fermentation. According to the present invention, the hermetia illucens larvae are adopted as the main raw material, the main raw material is ground into the slurry through the colloid mill, the small amount of the carbon source is supplemented, the Phaffia rhodozyma CGMCC NO.29217, the Bacillus coagulans CGMCC NO.5233 and the Clostridium butyricum CGMCC NO.4729 are subjected to the mixed bacteria synergistic fermentation, and the produced product can be widely used in the aquaculture and other application scenarios. Fermented hermetia illucens larva serous fluid is rich in astaxanthin, a fermented product has obvious acid fragrance and no peculiar smell, the total number of probiotics is larger than or equal to 5.0 * 10 < 8 > cfu / g, the pH value is smaller than 4.50, and the content of small molecule active polypeptide (acid soluble protein) of the fermented product is increased by 2.5-3 times compared with that before fermentation. Bacillus subtilis CGMCC (B) 63501 is used as indicator bacteria to detect that the content of bacteriostatic active substances after fermentation of hermetia illucens larva serous fluid is larger than 1.0 g / kg, pathogenic bacteria such as salmonella, vibrio parahaemolyticus and vibrio cholerae are not detected, and normal-temperature storage can be achieved. The preparation method is low in production cost, easy to operate, safe and free of residues, and the economic benefits of aquaculture can be improved.

This invention belongs to the field of bioengineering and carbon one-carbon resource utilization technology, specifically relating to a bacterial strain that produces acetic acid and acetate through syngas fermentation, as well as its applications and methods. The strain is *Clostridium yongdarii* (…). Clostridium ljungdahlii SL40, deposited at the Guangdong Provincial Microbial Culture Collection Center (GDMCC No. 67679) on January 15, 2026, represents a strain that achieves highly efficient H2 / CO2 conversion in syngas, with a maximum CO2 utilization rate of 1.8 g / L / h. The anaerobic fermentation process produces zero pollutants, enabling resource utilization and carbon reduction of industrial waste gas, and providing a new technological pathway for carbon resource conversion.

The invention relates to the technical field of microorganisms, in particular to mixed bacteria fermentation liquor as well as a preparation method and application thereof. Bacterial strains in the mixed fermentation liquor comprise clostridium butyricum, enterococcus faecium and bacillus. The preparation method of the mixed bacteria fermentation liquor comprises the following steps: culturing the strains in the mixed bacteria fermentation liquor in a mixed bacteria fermentation culture medium to obtain the mixed bacteria fermentation liquor; wherein the bacterial strains comprise clostridium butyricum, enterococcus faecium and bacillus. According to the mixed bacterium fermentation liquor and the preparation method and application thereof, coexisting culture of donkey source enterococcus faecium, donkey source bacillus and donkey source clostridium butyricum can be achieved through the mixed bacterium fermentation liquor, and meanwhile the mixed bacterium fermentation liquor has the excellent effects of producing compound enzyme and inhibiting pathogenic bacteria and can serve as a feed additive to improve the reproductive performance of a female donkey and improve the growth performance of a donkey foal; the occurrence of donkey foal diarrhea is reduced, so that the economic benefit of donkey breeding is increased.

The present application provides the use of a nanocomposite with a core-shell structure. Specifically, the present application provides the use of a nanocomposite with a core-shell structure in the preparation of a drug for preventing and / or treating Clostridium difficile infection and recurrent diseases caused thereby, wherein the nanocomposite comprises: ZnO nanoparticles as a core; mesoporous silica as a shell coated on the surface of the ZnO nanoparticles; an antibiotic loaded in the pore channel of the mesoporous silica; hyperbranched polylysine modified on the surface of the mesoporous silica; and a pH-responsive coating material as the outermost layer. The nanocomposite provided by the present application can achieve precise targeted release of the drug in the colon, and through the sequential and synergistic mechanism of "membrane breaking-sterilization-spore inhibition-repair", it can simultaneously solve multiple factors leading to CDI recurrence such as biofilm protection, spore storage, bacterial population destruction, mucosal damage, etc., and improve the treatment effect.

Provided is a carbonate-containing granular material production method with which it is possible to produce a carbonate-containing granular material at a cost lower than when using conventional methods.　The present invention has a carbonation step for bringing, into contact with a carbonation-target granular material at a maximum temperature of higher than 0°C to lower than 100°C, either water, a carbonation accelerator, and at least one among a carbon dioxide-containing substance, a carbonate ion-containing water, and a hydrogen carbonate ion-containing water, or a carbonation accelerator and at least one among a carbon dioxide-containing water, a carbonate ion-containing water, and a hydrogen carbonate ion-containing water. The total amount of water brought into contact in the carbonation step is 1 mass% or more of the carbonation-target granular material. The carbonation accelerator contains bacteria. The bacteria are at least one selected from the genus Bacillus, the family Bacillaceae, the class Bacilli, the family Clostridiaceae, the family Micrococcaceae, the family Microbacteriaceae, or the family Comamonadaceae.

A biological control method for centipede breeding belongs to the field of special economic animal breeding technology. This invention uses a compound probiotic strain of *Trichoderma longicornis*, *Streptomyces flavus*, *Bacillus subtilis*, *Bacillus licheniformis*, *Clostridium butyricum*, and lactic acid bacteria, which is fully incorporated into the breeding substrate. After 24-72 hours of static setting, the bacteria fully colonize and form a stable antibacterial state. The bacterial community can remain dormant during periods of nutrient deficiency, low-temperature overwintering, or year-end static setting, and will revive upon contact with organic matter. The method is supplemented with springtails and pink woodlice, which preferentially consume harmful bacteria, clean up uneaten feed, and primarily feed on and decompose centipede feces, forming a mutually beneficial symbiotic relationship with the biocontrol bacteria. Their feces further nourish the bacterial community, extending the stable effective period of the bacterial community from 2 months to 4-6 months or more. Simultaneously, probiotics and vitamins are added to the feed to strengthen the centipede's constitution. This invention constructs a three-dimensional synergistic prevention and control system that integrates matrix inhibition of pathogenic bacteria, environmental residue removal, and internal administration for enhanced protection. This system enables drug-free ecological disease control, significantly reduces the incidence of diseases such as green muscardine, and improves the survival rate of centipede farming. It is suitable for large-scale centipede farming.

The invention relates to bacillus velezensis Bv-GD2 and application of the bacillus velezensis Bv-GD2. The classification name of the bacillus velezensis Bv-GD2 is bacillus velezensis, and the preservation number of the bacillus velezensis Bv-GD2 is GDMCC No: 66722. The strain is wide in antibacterial spectrum and high in tolerance, has the enzyme production capacity and the antibacterial active substance production capacity, and can remarkably inhibit common intestinal pathogens such as clostridium perfringens, salmonella, staphylococcus aureus and escherichia coli, relieve intestinal lesions, improve the health degree of the intestinal environment and improve the growth performance of livestock and poultry.

The preparation method comprises the following steps: (1) compounding hermetia illucens larvae, whole tenebrio molitor and euphausia superba according to a mass ratio of (3-5): (2-4): 1, carrying out enzymolysis, and reacting with a yeast cell wall extract to form a glycopeptide compound; (2) coating the glycopeptide compound with a mannan oligosaccharide and chitosan solution to form microcapsules, and controlling the release rate of the microcapsules in simulated gastric juice to be lower than 30% and the release rate of the microcapsules in simulated intestinal juice to be higher than 80%; (3) co-fermenting the microcapsules, clostridium butyricum and pediococcus acidilactici, and adsorbing fermentation liquor on the porous starch; (4) performing dry mixing on the functional compound, the fermentation compound and the base material, and performing low-temperature puffing; and (5) sequentially spraying a vitamin D3 grease layer, a pectin-whey protein protective layer and a solution containing TGF-beta2 on the surfaces of the particles. Through cooperation of multiple processes, the immune function and intestinal health of pets are remarkably improved, and the product is good in stability and palatability.

The invention discloses a livestock and poultry breeding feed additive capable of reducing cost and improving effect. The livestock and poultry breeding feed additive is prepared from the following raw materials through compound fermentation: probiotic components: bacillus subtilis, clostridium butyricum and enterococcus; the enzyme preparation components comprise cellulose decomposition enzyme and xylanase; a fermentation medium: brown sugar and water; the traditional Chinese medicine auxiliary materials comprise wormwood, health sand and andrographis paniculata. The preparation method of the cost-reducing and effect-improving livestock and poultry breeding feed additive comprises the following steps of bacterium expanding culture, secondary fermentation and product treatment. The invention belongs to the technical field of livestock and poultry breeding, and particularly provides a livestock and poultry breeding feed additive and a preparation method thereof, and the livestock and poultry breeding feed additive can improve the intestinal microecological balance of livestock and poultry, improve the digestion and utilization rate of feed, enhance the immunity of livestock and poultry and reduce the breeding cost through scientific matching of probiotics, enzyme preparations, traditional Chinese medicine components and nutritional auxiliary materials.

The application discloses a kind of low morbidity feed for California bass antibiotic-free breeding and preparation method thereof, belong to aquatic feed field.The feed is composed of 60~80 parts of basic feed component, 10~20 parts of fermented plant protein, 2~6 parts of functional compound additives.Fermented plant protein is prepared by compound fermentation of butyric acid clostridium and bacillus subtilis from soybean meal, bran, rice bran, brown sugar and the like.Functional compound additives include pre-embedded material and microcapsule coated sodium butyrate, and the pre-embedded material is prepared by embedding treatment of astaxanthin, lutein and rosemary extract with β-cyclodextrin and gum arabic.The application significantly reduces the incidence of intestinal inflammation of California bass through the synergistic effect of fermented plant protein, pre-embedded antioxidant combination and microcapsule sodium butyrate, enhances antioxidant capacity, improves body color, improves the stability of active ingredients and feeding rate, and realizes low morbidity under antibiotic-free breeding conditions.

The present invention relates to methods and compositions for reducing the risk and severity of C. difficile infection. It is based, at least in part, on the discovery that a restricted fraction of the gut microbiota, including the bacterium Clostridium scindens, contributes substantially to resistance against C. difficile infection. Without being bound by any particular theory, it is believed that this is achieved through the biosynthesis of secondary bile acids.

This invention belongs to the field of microbial technology, specifically relating to a *Clostridium butyricum* strain, its culture conditions, and applications. This invention provides a novel *Clostridium butyricum* strain with strong resistance to high temperatures, acids, alkalis, and salts, exhibiting high biocompatibility and no significant toxic side effects or hemolytic activity. Therefore, the *Clostridium butyricum* H2-2 is a potential probiotic strain that can be used as feed or feed additive in pig, poultry, and aquatic feeds for animal husbandry, strengthening the intestinal barrier and enhancing animal immunity; it also holds promise for use as an additive in human food, pharmaceuticals, and health products.

To provide new and / or alternative therapeutic solutions which may alleviate the symptoms associated with Clostridium difficile infection, preferably intestinal infections, and / or cure the physiopathological conditions related to / associated therewith.SOLUTION: The present invention relates to the use of specific strains of Lactobacillus paracasei or of a composition comprising the strains for the prevention and / or treatment of a physiopathological condition related to / associated with Clostridium difficile infection, preferably intestinal infections, or so-called CD-associated disease (CDAD) or CD infection (CDI).SELECTED DRAWING: None

The invention discloses a feed additive for replacing antibiotics to treat chicken necrotic enteritis and application of the feed additive, and belongs to the technical field of feed. The effective component of the feed additive is the tannin extract from chestnut trees, and animal experiments prove that the tannin extract from chestnut trees can improve the production performance of broiler chickens with necrotic enteritis to a certain extent, improve the intestinal morphology and immune function of the broiler chickens with necrotic enteritis and improve the immunity of the broiler chickens with necrotic enteritis. Therefore, the chestnut tree tannin extract can replace antibiotics and has a good treatment effect on broiler intestinal inflammation caused by clostridium perfringens and coccidiosis.

Oxadiazole antibiotics that exhibit bactericidal activity against C. difficile vegetative cells. We screened a library of 75 oxadiazoles against C. difficile ATCC 43255. The findings from this collection served as the basis for the syntheses of an additional 58 analogs, which were tested against the same strain. We discovered a potent (MIC50=0.5 μg / mL, MIC90=1 μg / mL for 101 C. difficile strains) and narrow-spectrum oxadiazole (3-(4-(cyclopentyloxy)phenyl)-5-(4-nitro-1H-imidazol-2-yl)-1,2,4-oxadiazole; compound 57) that is not active against common gut bacteria or other tested organisms, but is selectively bactericidal against C. difficile and targets cell-wall synthesis. Other similarly effective oxadiazole antibiotics of formula I and II are described herein, several of which inhibit or prevent C. difficile spore germination.

The application discloses antagonistic Bacillus of Clostridium perfringens type G and application, the Bacillus is Bacillus sonorensis CB-6, and is classified and named as Bacillus sonorensis CB-6, the preservation number is CCTCC NO: M20251373, the preservation date is June 12, 2025, and the preservation unit is China Center for Type Culture Collection, which is located at Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province.The sterile fermentation supernatant of Bacillus sonorensis CB-6 provided by the application has neutral pH value, can inhibit Clostridium perfringens with high activity, the bacteriostatic effect is not dependent on organic acid such as lactic acid, the bacteriostatic effect is significant and stable, can significantly enhance the immunity of laying hens after Clostridium perfringens infection and improve the egg production rate, and solves the problem that laying hens are prone to necrotic enteritis during the egg production period and are not suitable for using antibiotics for prevention and control.

Compositions comprising aptamers capable of specifically binding to a surface protein of Clostridium difficile spore are provided. A method for detecting, enriching, separating, and / or isolating Clostridium difficile spores is provided.

An insoluble cell fraction obtained from plant cells cultured in a suspension composed entirely of natural molecules for the treatment of primary and recurrent Clostridium difficile infections is provided. A process for the preparation of the insoluble cell fraction is also provided.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus paralicheniformis CA10001 as well as application and a product thereof. The bacillus paralicheniformis CA10001 has been preserved in the China General Microbiological Culture Collection Center on February 22, 2024, and the preservation number of the bacillus paralicheniformis CA10001 is CGMCC No. 29876. The strain has relatively strong antibacterial ability, heat resistance, acid resistance and cholate resistance to clostridium perfringens, and can be used for improving feed quality and improving animal growth performance.

The invention discloses a method for inhibiting germination of clostridium spores and application, and belongs to the field of food microorganisms. The method for inhibiting germination of the clostridium spores comprises the step of adding beet red into a spore-containing system so as to inhibit germination of the spores. The beet red used in the invention is a natural colorant, and has the characteristics of no toxicity and no carcinogenicity; the method does not need to be compounded with other spore germination inhibiting agents for use, and is wide in use temperature range, single in component, convenient to operate and suitable for large-scale popularization and application.

The present application relates to the technical field of microorganism, specifically relates to an engineering bacteria, a construction method, an engineering bacteria medicine delivery system and purposes thereof, and provides an engineering bacteria, which comprises at least one coding gene A capable of coding interferon IFN-gamma; the chassis bacteria of the engineering bacteria is Clostridium butyricum; since Clostridium butyricum is an anaerobic gram-positive bacteria, its spores can resist adverse environment and enter the intestinal cavity through gastric juice, and in view of the hypoxic microenvironment of tumor, the engineering bacteria Clostridium butyricum spores of the present application can target the hypoxic microenvironment of tumor, and further can mildly express IFN-gamma, the expression amount is stable, and it is more safe and reliable.

The application discloses a kind of compound probiotic propagation methods and application, including the following steps: S1, in culture solution inoculation clostridium butyricum species carries out first fermentation, obtains first fermentation liquor;S2, inoculation lactobacillus plantarum species and Enterococcus faecium species carry out second fermentation, obtain second fermentation liquor;S3, inoculation yeast fungus species carries out third fermentation, obtains compound probiotic propagation liquor.The compound probiotic propagation liquor obtained in the application is added to pig feed for feeding, which can significantly improve the production performance of different pig groups, improve the skin redness and hair gloss of pig groups, increase the feed intake of pig groups, and reduce the breeding cost.

This invention discloses the Clostridium difficile phage recombinant lyase Lys51, its preparation method, and its application, relating to the field of genetic engineering technology. The invention involves introducing the recombinant plasmid pET-28a-SUMO-Lys51 into Escherichia coli BL21(DE3) competent cells via heat shock transformation; screening to obtain the recombinant engineered bacterium BL21(DE3)-pET-28a-SUMO-Lys51; constructing the recombinant plasmid pET-28a-SUMO-Lys51 and the recombinant engineered bacterium BL21(DE3)-pET-28a-SUMO-Lys51, further inducing the expression of Clostridium difficile phage recombinant lyase Lys51 in the recombinant engineered bacterium BL21(DE3)-pET-28a-SUMO-Lys51, and then purifying it to achieve the complete process for preparing Clostridium difficile phage recombinant lyase Lys51.

The invention belongs to the technical field of feed additives. The present invention provides a cattle and sheep feed additive and a preparation method thereof, the method comprises the following steps: S1, bacillus licheniformis and cellulose-pectin compound enzyme are uniformly mixed according to a mass ratio of 4: (1-1.2), a sodium alginate aqueous solution accounting for 35-40% of the mass of the mixture is added, and the mixture is uniformly stirred and dried to obtain enzyme compound aerobic bacteria powder; s2, lactobacillus plantarum, clostridium butyricum and xylooligosaccharide are mixed according to the mass ratio of 1: 1: (0.6-0.7) and stirred to be uniform, and xylooligosaccharide composite anaerobic bacterium powder is obtained; and S3, uniformly mixing the enzyme composite aerobic bacterium powder and the polyxylose composite anaerobic bacterium powder according to a mass ratio of 1: (1.2-1.3), thereby obtaining the enzyme composite aerobic bacterium powder. The additive prepared by the invention can improve the effect of the feed on promoting weight gain of animals, and improve the effect of the feed on preventing and treating diarrhea.

The invention discloses an intestinal microbial marker combination for noninvasive diagnosis of intrahepatic cholestasis and application of the intestinal microbial marker combination, and belongs to the technical field of medical treatment. According to the invention, by integrating metagenome sequencing and a machine learning algorithm, a group of intestinal microbial marker combination composed of 12 bacterial species is screened; the bacillus subtilis specifically comprises atypical virenoccus virens, Wilbionan isonibacterium, Brawtella, mucophilic protein anaerobic corynebacterium, bacteroides simplex, a clostridium AT4 strain, sargassum purpurascens, lawsonia sacchariflora, clostridium enteric, actinomycetes grignard, brassicaceae marsi Q3528 and bile vowenii. The AUC of a diagnosis model constructed based on the marker combination on an independent test set reaches 0.871, and the sensitivity and the specificity reach 86.7% and 81.3% respectively. The invention provides a non-invasive, simple, convenient and accurate auxiliary diagnosis tool for intrahepatic cholestasis, and has excellent clinical transformation prospects.