398 results about "Clostridium bacteria" patented technology

The invention relates to a eubacterium and clostridium preparation and application of the same, in particular to a microecological preparation which is prepared for replenishing butyric acid bacteria and butyric acid produced by intestinal tract by taking single eubacterium, single clostridium or a eubacterium and clostridium composition as a main active composition, and application of the same in treating related diseases through butyric acid production, and belongs to the field of biological medicine.

The invention discloses a microbial inoculum for decomposing lignocellulose and application thereof. The active component of the microbial inoculum consists of Alcaligenes faecalis, Bacteroides xylanisolvens, Clostridium xylanolyticum, Clostridium lentocellum, Flavobacterium aquatile, and Pseudomonas stutzeri. Experiments prove that the microbial inoculum has high degradation rate to the lignocellulose, and the degradation rate can reach between 60 and 70 percent. The microbial inoculum has low cost and simple preparation, breaks through the limitation that a purification bacterium and a clastic enzyme thereof cannot decompose the lignocellulose efficiently, provides a key technique for decomposing, saccharifying biomass containing cellulose and converting the biomass into an energy source, and has extensive application prospect in the field of decomposing the lignocellulose.

Disclosed are compounds of formula (I), which are of use in the treatment of bacterial diseases and infections, to compositions containing those compounds and to methods of treating bacterial diseases and infections using the compounds. In particular, the compounds are useful for the treatment of infection with, and diseases caused by, Clostridium difficile.

The present invention relates to a vaccine for the prevention of lactic acidosis in a vertebrate, said vaccine comprising at least one isolated microorganism, or fragment or fragments thereof, wherein said microorganism is capable of producing lactic acid within the gut of said vertebrate, and wherein said microorganism is selected from the group consisting of: Clostridium-like species, Prevotella-like species, Bacteroides-like species, Enterococcus-like species, Selenomonas species, non-dextran slime producing Streptococcus species and non-slime producing lactic acid bacterial isolates.

The invention discloses a clostridium butyricum having a diarrhea treating effect. The strain is named as clostridium butyricum Cb-2 which is conserved in China Center for Type Culture Collection with the conservation number of CCTCC M2011384, in November 9th, 2011. A test proves that the clostridium butyricum disclosed by the invention has strong acid producing capability especially butyric acidproducing capability; and the clostridium butyricum has obvious effects on diseases, such as intestinal tract flora infection, acute and chronic diarrhea, irritable bowel syndrome and non-ulcer dyspepsia, caused by various reasons, and has no toxicity and harm. Thus, the clostridium butyricum or clostridium butyricum powder disclosed by the invention is used as a raw material for preparing a micro-ecological preparation and used for replacing sodium butyrate to be applied; and when a specific application is carried out, a clostridium butyricum preparation can be singly used as an active component to be prepared into the micro-ecological preparation together with corn starch, or, the clostridium butyricum preparation is mixed with at least one of other bacterial preparations and the corn starch to prepare a composite micro-ecological preparation.

The invention aims to provide bacillus subtilis WL-04 and application thereof to livestock breeding. The preservation number of the bacillus subtilis WL-04 is CCTCC NO:M 2013343. The bacillus subtilis WL-04 can be used for remarkably inhibiting the growth of Escherichia coli, salmonella, clostridium pefringens and other conditional pathogenic bacteria of the intestinal canal of livestock, and particularly, the bacteriostasis rate of the bacillus subtilis WL-04 to Escherichia coli and clostridium perfingens is above 75%; the enzyme production is stable; the average activity of alpha-amylase is 22U/mL, and the average activity of neutral protease is 170U/mL; the reproduction of probiotics of intestinal canal can be promoted; high temperature for pelletizing can be endured; the influence of inverse environments with low-pH gastric fluid and high-concentration cholate is endured; the production performance of breeding animals can be effectively improved; immune organ development can be promoted. The bacillus subtilis WL-04 can be widely applied to livestock feeds as a feed additive.

The invention discloses high-butanol-yield clostridium beijerinckii, which is obtained by sampling, enriching, primary screening, separating and secondary screening. The strain is identified as clostridium beijerinckii which belongs to the class of clostridium beijerinckii gxas.28. The collection number of the strain is CCTCCM2011454. According to the invention, butanol is produced by using molasses as a raw material and by fermentation of clostridium beijerinckii. The butanol yield is 9.42 to 13.11g/L, and the ratio of the butanol to total solvent reaches 70 to 85 percent.

The invention relates to the field of biological medicine, in particular to an application of Rakicidins compounds in resistance to clinical pathogenic anaerobic bacteria. Pharmacodynamic experiments indicate that the Rakicidins compounds have good resistant effect on the clinical pathogenic anaerobic bacteria and have the resistant effect on vancomycin enterococcus infection diseases. The Rakicidins compounds can be used for treating diarrhea, enteritis, alimentary infection, oral infection or skin acne caused by clostridium difficile as well as diseases such as urinary tract infection, or skin soft-tissue infection and the like.

The invention discloses high-efficiency phosphate-solubilizing Clostridium butyricum, a preparation method and application thereof, belonging to the technical field of agricultural microorganisms. In the invention, one high-efficiency phosphate-solubilizing Clostridium butyricum A5-4 strain is obtained through separation, and the strain is preserved in China Center for Type Culture Collection (CCTCC for short), wherein the preservation number is CCTCC NO: Ma010052. The bacterium is separate from the substrate sludge of a septic tank and has the capacity of efficiently dissolving calcium phosphate and calcium phytate under the anaerobic condition. The bacterium has wide nutrient requirement range, easy cultivation, low cost and storage property and can not cause environmental pollution after being reapplied to the environment. The strain can be developed into a novel microorganism agent applied to aquatic crop cultivation or aquaculture.

The present invention relates to methods and compositions for reducing the risk and severity of C. difficile infection. It is based, at least in part, on the discovery that a restricted fraction of the gut microbiota, including the bacterium Clostridium scindens, contributes substantially to resistance against C. difficile infection. Without being bound by any particular theory, it is believed that this is achieved through the biosynthesis of secondary bile acids.

The invention provides a microorganism bacterium agent for subtracting organic sludge. The microorganism bacterium agent is characterized in that: the microorganism bacterium agent per milliliter contains more than 2.7*10<9> of live bacteria, and the pH value is 3-4; the microorganism bacterium agent comprises photosynthetic bacteria, bacillus mucilaginosus, lactic acid bacteria, fusarium, anaerobic clostridium, denitrifying bacteria, sphaerotilus and zymogenous filamentous fungus. The invention also provides a corresponding production method of the microorganism bacterium agent and a complex culture medium. According to the invention, a mode of combining a plurality of floras is adopted to reduce and degrade the organic sludge and organic pollutants in water in a river (lake) or a municipal sewage treatment plant, and a limit that the effect is reduced due to single strain variation easily caused by environment change is avoided.

The invention relates to a compound bacterium agent capable of decomposing cotton stalks quickly and a preparation method thereof. The compound bacterium agent comprises the following components in percent by cell content: 10-20% of clostridium thermocellum, 5-15% of desulfotomaculum, 5-35% of thermophilic bacillus licheniformis, 5-20% of butyrivibrio fibrisolvens, 25-35% of bacillus subtilis and 10-20% of lactobacillus thermophilus. The preparation method comprises the following steps of: crushing air-dried cotton stalks and the manure of pigs, cows and chicken till the water content is 40-60%, mixed stacking, mixing, stacking, turning, measuring the indexes such as temperature and cotton-stalk decomposing amount; taking compost into a triangular flask in proper time, adding physiological saline or PBS (Phosphate Buffer Solution) in a swinging table for vibration, taking the obtained mixture, standing, inoculating the bacterium solution according to the mixing quantity, putting the mixture into a culture medium for constant-temperature dark and static culture; taking the bacteria with strong decomposition as strains for next subculture repeatedly, and carrying out subculturing circularly and repeatedly for 30-60 times to obtain the stable compound bacterium system; and adding yeast powder and the like, spraying bacterium solution, and drying for multiple times to obtain the compound bacterium agent. The compound bacterium agent can realize high-efficiency decomposition for cellulose and hemicelluloses in the cotton stalks, and is used for the purposes of energy conversion, feeding and the like of the cotton stalks.

The invention relates to a bacillus series straw fast rotting agent and a using method thereof. The fast rotting agent comprises bacillus licheniformis, bacillus circulans, bacillus subtilis, clostridium pasteurianum, bacillus megatherium, jelly-like bacillus, clostridium acetobutylicum, bacteria macerans, bacillus polymyxa and nutrient solution and is used for treating straws to make biological organic fertilizer. The using method comprises the following steps: stacking the straws into a walkway with the width of 40 cm and the height of 30 to 50 cm; uniformly spraying 1.25 kilograms of the fast rotting agent into per ton of straws; controlling the water content of the straws to be 40 to 60 wt%; and rotting at normal temperature for 1 to 2 weeks, wherein when the straws change into brown or black brown, the wet straws are flexible and elastic when being held by hands and the dry straws are crisp and easy to break, a straw decomposition agent product with the nutrient substance content up to 59 weight percent is obtained and can direct serve as fertilizer. By adoption of the fast rotting agent, the straw rotting time is reduced from 15-20 days at present to 7-10 days.

The invention discloses a straw degradation acidification microbial inoculum and a manufacturing method thereof. Active ingredients of the composite microbial inoculum for degrading straw are cellulomonas flavigena, clostridium cellulolyticum, bacillus subtilis and bacillus cereus. The microbial inoculum breaks through the limitation that fungi and lytic enzymes thereof cannot quickly and efficiently decompose natural cellulose, a key pretreatment technology is provided for straw biogas fermentation, and the straw degradation acidification microbial inoculum has wide application prospect in the field of preparation of biogas from lignocellulose raw materials such as the straw.

The invention aims to provide a method for preparing a compound bacterium agent for livestock breeding. The method comprises the following steps: fermenting bacillus subtilis, bacillus natto, bacillus coagulans, lactobacillus rhamnosus and clostridium butyricum, adding a carrier, performing plate-frame pressure filtration, adding a protective agent for emulsifying and spray drying, and finally, matching according to effective bacteria numbers of the five strains. The method is simple in process and easy to operate, the carrier and protective agent adopted in the preparation process are edible components, the safety of the bacterium agent can be guaranteed, and a reasonable proportion among the five strains can be guaranteed in the method, so that the strongest synergistic effect is achieved, and the method is suitable for large-scale popularization and application.

The invention discloses a method for preparing a clostridium butyricum feed additive and a clostridium butyricum feed additive prepared by the method. The method for preparing the clostridium butyricum feed additive is characterized by adopting clostridium butyricum (the clostridium butyricum is preserved in the China Center of Industrial Culture Collection and the serial number is CICC 20036). The method comprises the following steps: such materials as tryptone, beef extract, yeast cream, glucose, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, magnesium sulphate, calcium chloride, ferrous sulfate, cysteine hydrochloride and the like are prepared into a dedicated liquid culture medium for seeds; strains are grafted into the culture medium for activating seeds; nitrogen source, carbon source and the like are prepared into a solid anerobic fermentation culture medium; and the seed liquid is grafted into the solid anerobic fermentation culture medium for solid state fermentation and then is dried and sieved. By utilizing the cheap nitrogen and carbon sources to form the medium, the invention provides a novel method for large-scale and industrial production of clostridium butyricum; and the method reduces the cost effectively and increases the benefits of plants.

The invention provides biological fermentation type functional feed for bighead carp. The feed is mainly prepared from components in parts by weight as follows: 30-70 parts of fish meal, 300-600 partsof distillers' grains, 200-400 parts of soybean meal, 50-100 parts of bran, 70-130 parts of rice bran and 15-35 parts of a premix. The premix comprises a fermenting agent and components capable of promoting bighead carp culture such as multi-vitamins for fish and compound ore elements for fish, and the fermenting agent comprises saccharomyces cerevisiae, lactobacillus plantarum, bacillus coagulans, enterococcus faecalis and clostridium butyricum. The invention also provides a preparation method of the biological fermentation type functional feed for bighead carp. The method mainly comprises the following steps: uniformly mixing all the components, directly placing the mixture in a net bag with a respiratory membrane, and fermenting the mixture for 7-10 days to obtain the feed. The biological fermentation type functional feed for bighead carp is simple to prepare, not high in equipment requirement, high in operability and easy to promote and use in large-scale farms or aquaculture cooperatives.

The present invention provides methods for the treatment or prophylaxis of a microbial infection of the lower intestine or colon in a subject, the method comprising administering to the subject a type-B lantibiotic. In particular, the invention provides methods for the treatment or prophylaxis of a Clostridium difficile infection. The type-B lantibiotics may include compounds selected from the group consisting of mersacidin, actagardine, plantaricin, planosporicin, ruminococcin, antibiotic 10789, michiganin and haloduracin, and derivatives and variants thereof.

The present invention provides: a Clostridium butyricum strain having immune enhancement and antiviral activities; an immune enhancement and antiviral composition, a probiotic composition, an antibacterial composition and a composition for producing butyric acid or acetic acid, wherein all the compositions contain, as an active ingredient, the strain or a culture solution thereof; and a method for producing butyric acid or acetic acid, comprising a step of culturing the strain.

An antimicrobial composition and a method for administering the antimicrobial composition through the water or feed of livestock, wherein the antimicrobial composition is made up of lysozyme and various other agents that act synergistically with lysozyme, such as, dried egg powder, albumen, a sequestering agent and/or a lantibiotic. The composition is used to inhibit the growth of, and diseases and epidemiological significant effects caused by, Clostridium perfinigens, E, coli and Salmonella, in the gut of livestock. More particularly, the antimicrobial composition and method relate to a feed additive that can be administered to poultry and/or swine through their feed. The use of such a feed additive may also inhibit other enteric pathogens that may be present in the gut of livestock.