Culture collection process
A kind of strain preservation and strain preservation technology, applied in the field of strain preservation, can solve the problems of people who have no relevant knowledge, such as illness, insolvability, and environmental pollution, and achieve the effects of prolonging the preservation time, improving production efficiency, and reducing damage
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The dry powder medium was added with purified water to make a semi-solid medium. In terms of mass, the composition of the dry powder medium is as follows:
Peptone 10
Sodium chloride 5
Beef Dip Powder 3
Agar 4
Divide and sterilize. Each test tube is filled with 10-20ml of culture medium, placed vertically in the incubator and cultured for 2 to 3 days, observe and test, and proceed to the next step after confirming the sterility. This step is to ensure that the purity of the inoculated bacteria is not destroyed by the bacteria. Pick a small amount of the prepared strain with an inoculation needle, puncture and inoculate it into the semi-solid medium, and puncture and inoculate 3 points parallel to the circular plane to ensure that the strain is surely inoculated into the medium. Pour 1~2cm high sterile liquid paraffin, stopper the test tube firmly, seal the bottle with a sealing film, and store it vertically in a refrigerator at 6~8℃.
Table 1 shows the relative bacterial concen...
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