182 results about "Asepsis" patented technology

The invention relates to a method for the plant tissue culture, in particular to the tissue culture method and application of the aoectochilus formosanus. The invention provides a culture method of aoectochilus formosanus tissue culture seedling comprising the steps that: the induction culture is performed by taking the wild aoectochilus formosanus as the raw material, then by means of hormone-free culture, the asepsis culture line of the aoectochilus formosanus tissue culture seedling is obtained; after that, the tissue culture seedling system which is suitable for a large-scale production is established, while the essential effective composition (amylase, amino acid) of the culture is close to the wild aoectochilus formosanus and stable. The main content comprises the configuration of the tissue culture seedling medium; the culture conditions of the tissue culture seedling; and the planting technology and usage of the tissue culture seedling. The invention solves the problem of the lack of natural resources of the wild aoectochilus formosanus.

The invention discloses a negative pressure ambulance used to prevent nosocomial infection, containing car body, hermetic board cabin connected with car body chassis, control device; the hermetic board cabin is consisted of nose functional cabin and medical afterhold; the nose functional cabin is the flow direction of air controlling cell, the medical afterhold contains cabin air inlet chamber, cabin exhaust chamber, also a medical equipment in cabin; the medical afterhold sets closable port; the cabin air inlet chamber of medical afterhold and air mixing chamber of nose functional cabin can be connected, the cabin exhaust chamber of medical afterhold and the air filtration disinfection chamber can be connected. The air flow of hermetic board cabin has three kinds of state, the air in cabin can all realize directional airflow and pressure gradient. This car can be used as normal ambulance, high pathogenic infectious patient ambulance, various diseases checking car, mobile p3 lab, carrier vehicle of biological bacteria danger foe things; it can be asepsis operation car after switching, high infectivity, strong virulence weapon battlefield command, arm carrying car.

The invention discloses a preparation method of diet health care preserved medlar. The method comprises the technical steps of material selection, color protection, chowchow, drying, aseptic packaging, vacuum packing, etc., wherein, the chowchow is calculated based on the mass of 100 portions of preserved medlar. The persevered meldar after color protection is added with 50 potions to 100 portions of sirup, 30 portions to 60 portions of honey and 5 portions to 10 portions of licorice extract, the process of chowchow lasts for 30 mins to 240mins. The sirup is a mixture of starch sirup and white sugar and / or xylose, wherein, the dosage of the starch sirup accounts for 10 percent to 50 percent of the total weight of the sirup, of which the concentration is 35wt percent to 60wt percent. The method has simple preparation technique with high effect and asepsis, thereby being applicable to industrialized production and favorable for popularizing. The preserved fruit prepared by the method has unique taste and flavor and is rich in nutrient components that are beneficial for human body.

The invention discloses an injectable type collagen-based soft tissue filling material and preparation method thereof, wherein the filling material mainly comprises collagen 0.5-20%, heparin 0.1%-10%, cell growth factor 0-1%, cushion liquid 70-99.4%, and its preparation comprises extracting soluble collagen from animal connective tissue, cutting off antigen deciding family from the collagen molecules through proteinase hydrolysis, purifying treatment, crosslinking by utilizing safety and nontoxic crosslinking agent, charging biologically active components, kneading the mixture repeatedly into grease form, sealing under asepsis condition and storing at low temperature.

The invention provides a method for cultivating plant tissue under non-aseptic condition, wherein the whole procedure of plant tissue cultivation is conducted under the natural ambient condition of non-asepsis, the method consists of adding disinfection and bactericidal agent into the culture media, preparing bacterium suppression culture media, inoculating under the natural ambient condition of non-asepsis, and placing the culture material in natural ambient condition meeting plant tissue culture.

An intelligent urinary bladder micturition rehabilitation device is installed between an indwelling catheter and a urine bag and comprises a three-way connector, an extrusion type electromagnetic valve, a pressure sensor and a controller, the three-way connector is provided with an inner chamber, an inlet end, an outlet end and a detection end, the inlet end, the outlet end and the detection end are communicated with the inner chamber, the inlet end is communicated with the indwelling catheter through a catheter, the detection end is communicated with the pressure sensor through a capillary tube, and the outlet end is communicated with the urine bag through an evacuating catheter; an air filter element beneficial to isolating urine from air is further arranged in the middle of the capillary tube, the extrusion type electromagnetic valve is provided with a mechanical switch capable of controlling the evacuating catheter to be opened, and the controller can control opening or closing of the extrusion type electromagnetic valve according to urine pressure output by the pressure sensor. According to a micturition monitoring method used through the intelligent urinary bladder micturition rehabilitation device, pressure of the urinary bladder is measured through urine in the catheter, opening and closing of the catheter are further controlled, the automatic micturition control function is achieved, and the advantages of convenience, sanitation, asepsis, non-pollution and no-cleaning are achieved.

The invention discloses a method for producing anoectochilus formosanus by tissue culture, comprising the following steps: frond selection and asepsis, cluster bud inducing culture and large-scale culture. The key to the technique is characterized in that two different culture media are applied to the inducing culture and the large-scale culture, wherein the inducing culture medium is as follows: H culture medium+ 10-50g / L of cane sugar + 0.1-3.0mg / L of alpha-naphthylacetic acid+ 0.1-5.0mg / L of 6-benzyladenine, and pH value is 5.0-7.0; the large-scale culture medium is as follows: B5 culture medium+ 20-40g / L of cane sugar+ 6-8g / L of agar+ 2.0-3.0g / L of active carbon+ 1.0-2.0mg / L of alpha-naphthylacetic acid+ 2.0-3.0mg / L of benzyladenine, and pH value is 5.5-6.5. The method is easy to operate, features low production cost, high output, high quality and no pollution to the environment and can realize batch production.

The invention discloses a production method for high-density pure arbuscular mycorrhizal fungal spore, which belongs to the technical field of microorganism culturing, and comprises the following technical steps of: 1) culturing carrot root tissues converted from agrobacterium rhizogenes plasmid DNA; 2) culturing the carrot root tissues converted from agrobacterium rhizogenes plasmid DNA and glomus intraradices together in an improved synthetic culture medium; 3) taking a culture containing the root, hypha and spore infected by the arbuscular mycorrhizal fungus and transplanting the culture to a special culture box for culturing; 4) collecting the spore and mycelium; and 5) storing the arbuscular mycorrhizal fungal spore. Compared with the prior art, the production method for high-density pure arbuscular mycorrhizal fungal spore has the advantages that: 1, the culture medium is an asepsis environment with low cost, which not only can meet the growing of a host plant, but also is suitable for the growing of AM epiphyte; 2, the nutrition requirements for the growing of the host plant are low and the host plant can grow and be cultured on the synthetic culture medium; 3, the spore cultured by the method is a non-pollution pure culture; and 4, the produced spore is stored in liquids and the activity can be maintained by above 90 percent.

The invention provides an induction rapid-propagation culture method for Photinia fraseri in-vitro leaf somatic embryos, comprising the following steps: (1) building a regeneration system of a Photinia fraseri asepsis test tube seedling; (2) building a high-frequency rapid-propagation system induced by an in-vitro somatic embryo and an adventitious bud; and (3) rooting, acclimatizing and transplanting for the test tube seedling. The induction rapid-propagation culture method for Photinia fraseri in-vitro leaf somatic embryos is characterized in that coordinated sets of measures of selecting a proper explant, improving a basic culture medium and ingredients, regulating culture conditions and the like are adopted, and the Photinia fraseri of which the seed is difficult to breed is subjected to path rapid propagation by the somatic embryos; the plant transplantation survival rate is higher than 95%; the germchit grows strongly to solve the problem that an excellent germchit quality degenerates, and a great quantity of purified and rejuvenated detoxification nursery stock with good quality can be provided for production; and in addition, and ideal receptor system is provided for breeding new specimens for Photinia fraseri by genetic transformation or mutation breeding.

The invention provides a method for preparing a decidua mesenchymal stem cell. The method comprises the following steps: performing placenta asepsis by taking the placenta of a male full-term fetus as a raw material; separating a decidual tissue; identifying the decidual tissue and identifying whether the decidua mesenchymal stem cell comes from a maternal tissue; culturing the decidua mesenchymal stem cell; freeze-storing the decidua mesenchymal stem cell; and reviving the decidua mesenchymal stem cell. The method provided by the invention has the technical characteristics that the sampled fetus is male fetus placenta; after the decidual tissue is obtained, sex determination is performed to identify whether the decidual tissue comes from a parent body and is free of pollution of a daughter tissue; by adopting a bacterial pollution prevention method, the pollution possibility is reduced from a sampling source; the surface is washed for a plurality of times, so that the pollution possibility is effectively reduced; a single enzyme is used for digestion to simplify the procedures; a serum-free medium is used for culture to reduce the use of an animal source component; the cell is stable in performance; a long-term in-vitro culture process of the decidua mesenchymal stem cell can be maintained; and the cellular morphology, multiplication capacity, MSC surface marking expression capacity, differentiative capacity and the like of the cell can be maintained.

The invention discloses the preparing technology of aseptic package material, comprising the following steps: opening air-cleaning system, disinfecting the clean area, making the inflation film manufacturing machine and operating environment achieve ten thousand classes grade; filtering the nitrogen and air, making the nitrogen and air achieve hundred classes grade; cleaning the polythene particle whose melting index is 0.2-0.4, adding it into hopper isolated from the clean area, then sending it into extrusion machine by closed conduit; starting inflation film manufacturing machine, indirectly heating polythene particle in extrusion machine, raising resin, inflating and frothing to form tubular film; cooling, and getting flat pipe aseptic film product and aseptic pocket product. The product possesses the good indexes of clarity, fusibility and microbe limit, meeting the aseptic package requirement of asepsis ejection medicinal powder and possessing the good social and economic benefit and environmental benefit.

A surgical instrument kit (10) and method of providing sterile instruments for spinal surgery, the instrument package having a spinal implant (20) suitable for insertion into a portion of the spine, instruments (12) suitable for spinal surgery, and A package (14) adapted to contain a spinal implant (20) and instruments (12) to maintain sterility prior to spinal surgery.

The invention discloses an early-flue-cured tobacco quality improving method with eurotium cristatum. The method comprises the steps that (1) the eurotium cristatum is cultured in an activation mode; (2) conidium bacterial colony culturing is carried out, a conidium bacterial colony culture medium is prepared and is formed into a flat plate, bacterial colonies of bacterial strain slopes from the step (1) are planted on the flat plate in a point mode and are cultured for 5-7 days in an inverting mode until the bacterial colonies overgrow on the flat plate; (3) spore suspension is prepared, conidium generated according to the step (2) is inoculated and is washed off with asepsis water and then is prepared to be the spore suspension which is used as inoculant to be evenly sprayed on early-flue-cured tobacco. When processing conditions are that culturing temperature is 30 DEG C and spore concentration is 106 and 107, aroma of the early-flue-cured tobacco becomes good, impurity odor and irritation are relieved, smoke sweet feeling is improved and is fine, pure and mild, after taste is pure and comfortable, nicotine is lowered, and whole tobacco quality is improved.

The invention relates to a pleione test tube breeding ball production technique, which consists of employing artificial nutrient solution and artificial culture, improving culture temperature, exercising the sprouts at low temperature, obtaining large quantity of tube seed balls and tube sprouts with asepsis sowing method, forming dormancy bulbodium through induction, and taking the bulbodium out of the bottle for convenient transportation, easy cultivation, and high survival rate.

The invention is concerned with a microorganism feedstuff additive to promote the growth of eels, especially to improve the compound function to liver. Culture one or some of the selected lichen bacillus, plant lactobacillus, lactobacillus casei and beer yeast. Carry domesticationhe and train the multiple bacteria and zymosis with culture medium and asepsis water, dry quickly with low temperature through drying mechine, add special carrier, crush and sift out to get production. It has the greatest amout of live bacterium and active metabolized produce, and the production has microorganism with high concentration and abundant active metabolized stuff. Those bacteria and metabolized stuff can improve the intestines function of eels to speed the composing of bile and special protein divided form liver. It also improves the water for living and it is a kind of zoology agriculture production.

The invention provides a method for inducing protocorms by utilizing dendrobe tissues. The method comprises the following steps: (1) carrying out routine asepsis on the obtained dendrobe tissues, wherein the dendrobe tissues are from the stem tip, root tip or tender stem section of dendrobe; and (2) carrying out induction culture on the dendrobe tissues subjected to asepsis in an induction culture medium firstly at the temperature of 23-25 DEG C and under a dark condition for one week and then at the temperature of 23-25 DEG C and under an illumination condition for 20-30 days with the illumination period of 12h / day, thus obtaining the protocorms. The method provided by the invention has the following main beneficial effects: (1) the method is simple in operation, low in production cost and less in environmental pollution; (2) the induction success rate is high, wherein the induction rate based on stem section culture is generally about 50%, the induction rate based on root tip culture can reach 60%-70%, and the best induction success rate based on the stem tip culture can reach about 75%; (3) the culture cycle is short, a large number of protocorms can be obtained within less time, and the induced protocorms can be directly subjected to aseptic culture so as to obtain a large number of seedlings; and (4) tissue culture is directly utilized, thus the characters of parents are well maintained.

A method for manufacturing an asepsis bonsai relates to a manufacture method of an ornamental gardening bonsai; that is, an aseptic seedling cultivated by isolated culture and decorative material are placed in an aseptic transparent container; as the water and nutrient are provided by a special culture medium, the aseptic seedling in an environment isolated from the outside can gradually grow and even blossom. Only by being placed in an environment with the normal temperature and under common scattered light, the asepsis bonsai can grow and develop in the transparent artware bottle container. People can observe the whole process of rootage, germination and blossoming. The aseptic seedling does not have any diseases and insect pests, does not need daily maintenance, and is environment-friendly, sanitary and beautiful; the ornamental period reaches more than 6 months. The asepsis bonsai not only is a recreation item, but also can help middle and primary school students recognize and understand plants.

The invention belongs to the technical field of plant reproduction and mainly provides a sarracenia asepsis sowing and culture quick reproduction method which comprises the following steps: the collection of seeds, the asepsis sowing, the enrichment culture of cespitose shoots, the culture of sound seedling, the culture of rootage, and the transplanting. The basic culture medium is as follows: selecting 1 / 6MS-1 / 3MS culture medium, and adding 20-30g / L of cane sugar or white sugar and 7-9 g / L of agaragar and having the pH of 5.6-5.8. The invention has quick reproduction speed, uniform production sturdiness and high transplanting survival rate, and is suitable to the massive production because a large number of better culture seedlings are formed in a short period.

The invention discloses a tissue culture and regenerated plant in vitro mycorrhization method for pinus massoniana, which belongs to the technical field of biotechnology and modern forestry. The method comprises the following steps of: taking a terminal bud of an aseptic seedling of the pinus massoniana as an explant, inducing to produce a large quantity of multiple shoots with strong growth first, further inducing the generation of an adventitious root, transferring a plant with the macroscopic adventitious root onto a perlite substrate under the condition of asepsis after the induction of the adventitious root is finished, and inoculating ectomycorrhizal rungi Pisolithustinctorius at the same time to realize the mycorrhization in a bottle. The mycorrhization improves the quality of a root system of the adventitious root of a tissue culture regenerated plant of the pinus massoniana, and ensures that the average lateral root number and the average root length are greatly improved. The mycorrhization also makes the state of the adventitious root greatly changed, and a fungal mantle obtained by closely arranging more than ten layers of mycelia is formed on the surface of the adventitious root. Through the method, the induction rate of the multiple shoots of the pinus massoniana reaches 95 percent, the proliferation rate reaches 430 percent, the rooting rate reaches 85 percent, and the transplantation survival rate is improved to more than 85 percent from 65 percent.

The invention discloses a device (401) for cleaning gas in blowing machines for shaping plastics material pre-forms to form plastics material containers. According to the invention at least one gas-processing device (416), which is capable of filtering the gas in a sterile manner and / or of reducing a quantity of an oxidative disinfecting / sterilizing agent contained in the gas flowing through and / or flowing past, is arranged in at least one gas line (402, 402a, 402b) which is a gas supply line (402a) or a gas removal line (402a) of a blowing station (8). The device for cleaning gas can filter gases in a manner of asepsis and / or can reduce the mass of oxidized disinfectant and / or bactericide contained in the flowing gases.

The present invention relates to a potato detoxified sprout sterile circulation germ plasm preservation method. Said method includes the following steps: using existent tuber tip tissue culture technique to obtain detoxified sprout, utilizing induction process to obtain test tube miniature potato, and it is characterized by that placing said test tube miniature potato into a culture vessel, making low-temperature preservation for 200-360 days at 3-5deg.C, then taking out said test tube miniature potato, under the condition of that the temperature is 20-25deg.C and illumination is 2000-4000 lux making culture for 40-60 days to obtain sterile sprout, under the condition of that temperature is 18-20deg.C culturing said sterile sprout for 60-80 days to obtain miniature potato, repeating above-mentioned operation so as to implement the detoxified potato sprout circulation germ plasm preservation.

The invention provides an X-ray locating film pasting component which comprises an X-ray-transmission film, X-ray-non-transmission filaments, medical pressure-sensitive adhesive and release paper. The filaments are arrayed regularly and are buried in the film. The medical pressure-sensitive adhesive is placed on one face of the film. The release paper is placed on the surface of the medical pressure-sensitive adhesive. The X-ray locating film pasting component is simple in structure, asepsis is achieved, skin stimulation is avoided, the component is disposable, the component can be cut open by a scalpel or be punched by a needle, and the component also has the effect of an operative incision protecting film. The invention further provides a manufacturing method of the X-ray locating film pasting component. The X-ray locating film pasting component is used in a minimally-invasive technology, accurate locating can be provided, an operator can be prevented from being hurt by X rays, meanwhile, X-ray damage on a patient is lowered, and obstacles are greatly removed for development of the minimally-invasive technology.

The invention discloses a novel chrysanthemum breeding method, which includes the following steps: 1) the young flower buds whose diameters are 0.45-0.55cm from the naturally grown small violet chrysanthemum plants are scissored as the explants; 2) the flower buds are rinsed by soapy-water, soaked by alcohol, sterilized by sodium hypochlorite in turn, and then rinsed by asepsis distilled water; 3) the sterilized flower buds are sucked up water,, induced and separated in a cultivating bottle; 4) the separated tube seedlings are cultivated into young plants; 5) the young plants are transplanted into a bowl filled with cultivation mud for breeding; 6) the grown plants whose leaf is ovate-lanceolate and stem is rhombuses, and inflorescence is verticillaster are selected among the survived transplanted plants, namely the variant 04M; 7) after sexual hybridization or asexuality graft of the variant 04M, then the novel chrysanthemum is obtained. The chrysanthemum cultivated with the method of the invention is verticillaster.

The invention provides a rapid breeding method of Cattleya by improving the current flow of production, the method comprises fluid culturing on earlier stage, so as to promote the seed to grow fast, then directly inoculating the original bulbodium to the sprout culture medium for sprout production in one step. The method can reduce culture cost and time greatly, and can be applied to Catteva genus, near source bigeneric hybid seedling production and breeding works.

The present invention relates to a device that eliminates germs and bacteria from your hands. The device is especially designed to achieve total asepsis of the hands of people working and / or should have access to certain sectors of industries manufacturing and / or fractionation of food, medicinal products, testing laboratories industry biological, chemical industries in general, operating rooms, etc. where it is required to maintain strict hygiene and asepsis conditions.

The invention relates to the technical field of medicament, in particular to inonotus obliquus extracellular and intracellular mixing crude polysaccharide with a function of strengthening immunity, which is extracted from a submerged fermentation product of medical fungus inonotus obliquus liquid. The method comprises the following steps: taking slant strain under an asepsis condition, and inoculating the slant strain into a plate filled with fresh comprehensive solid culture medium; pumping and filtering a secondary fermentation product through primary fermentation and secondary fermentation to obtain inonotus obliquus mycelium and fermentation supernatant; and preparing the inonotus obliquus extracellular and intracellular mixing crude polysaccharide through concentration and water extraction and alcohol precipitation. In vivo experiments prove that the inonotus obliquus extracellular and intracellular mixing crude polysaccharide can prolong the surviving time of mice hepatocellular carcinoma H22 and S180 ascites tumor mice, and can obviously improve the thymus gland index and spleen index of the mice. Furthermore, the inonotus obliquus extracellular and intracellular mixing crude polysaccharide can obviously improve secretion of IL-2, TNF-alpha cell factors in the blood serum of the tumor-bearing mice, and can be used for preparing new immunity intensifying preparation. The invention opens up a new way for development and utilization of medical fungus resources.

The invention relates to a medical cotton swab asepsis storage box, and discloses a storage and taking device through which cotton swabs can be conveniently taken out and bacterial pollution can be effectively prevented. The medical cotton swab asepsis storage box is characterized in that a fixing frame is arranged on upper edges on the left side and the right side of a main box through fixing grooves, a limiting plate is arranged on the fixing frame and parallel to the bottom of the main box, a cover plate is arranged on the main box and located below the limiting plate, the cover plate is provided with a taking outlet, a taking-out cover is hinged to the portion, on one side of the taking outlet, of the cover plate through a connection strip and located above the taking outlet, multiple partition plates are distributed on the cover plate at equal intervals and located in the main body, the distance between every two adjacent partition plates is slightly larger than the diameter of each cotton swab, multiple baffles are evenly distributed on the taking-out cover and arranged between the partition plates in a one-to-one corresponding mode, the taking outlet is divided by the baffles into multiple small windows, multiple shifting needles are evenly arranged on the cover plate and located between the partition plates correspondingly, and the shifting needles are located on one side of the taking outlet.

The present invention provides a cell analysis technology based on a film fiber material micro fluidic chip. The technology mainly comprises three basic aspects: 1, a film fiber material micro fluidic chip production technology, 2, an asepsis technology and surface modification of the film fiber material micro fluidic chip, and 3, cell culture, stimulation, and analysis and evaluation of the film fiber material micro fluidic chip. The technology has advantages of simple operation, low cost, low reagent and cell consumption, and the like, and is a new technology widely used for various cell analyses.

The invention discloses a blood microorganism culture flask which comprises a flask body, a flask cover, chemoreceptors, a liquid culture medium and an absorption resin, wherein an asepsis environment is provided in the culture flask; the flask body is a cylindrical plastic container; a layer of chemoreceptors are arranged at the bottom of the flask body; the chemoreceptors are high-molecular polymer semipermeable membranes which are solidly adhered to the flask bottom. By utilizing the culture flask provided by the invention, a technical difficult problem of a full-automatic blood culture instrument is solved, full-automatic culture and detection on blood microorganism are realized, the detection sensitivity is improved, and the positive detection time is shortened.