Inonotus obliquus extracellular and intracellular mixing crude polysaccharide with function of strengthening immunity

Inonotus obliquus, a technology for enhancing immunity, which is applied in the field of medicine and can solve the problems of high price of Inonotus obliquus, limitation of development and utilization of Inonotus obliquus, and the like.

Active Publication Date: 2010-08-18
王琦
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] The present invention provides a mixed intracellular crude polysaccharide of Inonotus obliquus with the effect of enhancing immunity and its medicinal application, so as to solve the problem that wild Inonotus obliquus is very expensive and the development and utilization of Inonotus obliquus is limited

Method used

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  • Inonotus obliquus extracellular and intracellular mixing crude polysaccharide with function of strengthening immunity
  • Inonotus obliquus extracellular and intracellular mixing crude polysaccharide with function of strengthening immunity
  • Inonotus obliquus extracellular and intracellular mixing crude polysaccharide with function of strengthening immunity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] Example 1 Preparation of the tablet of the present invention

[0094] 1. Under aseptic conditions, take the slanted strain and insert it into a fresh comprehensive solid medium plate, the culture temperature is 26 ° C, and cultivate for 240 hours; the comprehensive solid medium formula (g / 100ml): potato 16, glucose 1.2, agar 1.5, MgSO 4 0.12, KH 2 PO 4 0.28, VB 1 0.8mg is dissolved in water to the required volume, and the pH value is natural;

[0095] 2. Primary fermentation: Under aseptic conditions, punch holes at the edge of the colony with a 6mm puncher, use an inoculation shovel to take 6 pieces of bacterial cake and put them into a 250ml shake flask containing 100ml of liquid medium, and place them at 26°C , in a shaker with a rotating speed of 120 rpm / min, the secondary fermentation culture was carried out after culturing at a constant temperature for 210 hours; the liquid medium formula (g / 100ml): potato 16, glucose 1.2, MgSO 4 0.12, KH 2 PO 4 0.28, VB 1...

Embodiment 2

[0099] Example 2: Preparation of granules of the present invention

[0100] 1. Under aseptic conditions, take the slanted strain and insert it into a fresh comprehensive solid medium plate, the culture temperature is 28 ° C, and cultivate for 300 hours; the comprehensive solid medium formula (g / 100ml): potato 21, glucose 2.4, agar 2.0, MgSO 4 0.16, KH 2 PO 4 0.31, VB 1 1.0mg is dissolved in water to the required volume, and the pH value is natural.

[0101] 2. Primary fermentation: Under aseptic conditions, punch holes at the edge of the colony with a 6mm puncher, use an inoculation shovel to take 8 pieces of bacterial cake and put them into a 250ml shake flask containing 120ml of liquid medium, and place them at 28°C , in a shaker with a rotating speed of 150 rpm / min, carry out secondary fermentation culture after 260 hours of constant temperature cultivation; the liquid medium formula (g / 100ml): potato 21, glucose 2.4, MgSO 4 0.16, KH 2 PO 4 0.31, VB 1 1.0mg is disso...

Embodiment 3

[0105] The preparation of embodiment 3 capsule of the present invention

[0106] 1. Under aseptic conditions, take the slanted strain and insert it into a fresh comprehensive solid medium plate, the culture temperature is 29 ° C, and cultivate for 360 hours; the comprehensive solid medium formula (g / 100ml): potato 26, glucose 3.2, agar 2.5, MgSO 4 0.2, KH 2 PO 4 0.35, VB 1 1.2mg is dissolved in water to the required volume, the pH value is natural;

[0107] 2. Primary fermentation: Under aseptic conditions, punch holes at the edge of the colony with a 6mm hole punch, use an inoculation shovel to take 10 bacterial cakes and put them into a 250ml shake flask containing 150ml of liquid medium, and place them at 29°C , in a shaker with a rotating speed of 185 rpm / min, carry out secondary fermentation culture after culturing at constant temperature for 300 hours; the liquid medium formula (g / 100ml): potato 26, glucose 3.2, MgSO 4 0.2, KH 2 PO 4 0.35, VB 1 1.2mg is dissolved...

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Abstract

The invention relates to the technical field of medicament, in particular to inonotus obliquus extracellular and intracellular mixing crude polysaccharide with a function of strengthening immunity, which is extracted from a submerged fermentation product of medical fungus inonotus obliquus liquid. The method comprises the following steps: taking slant strain under an asepsis condition, and inoculating the slant strain into a plate filled with fresh comprehensive solid culture medium; pumping and filtering a secondary fermentation product through primary fermentation and secondary fermentation to obtain inonotus obliquus mycelium and fermentation supernatant; and preparing the inonotus obliquus extracellular and intracellular mixing crude polysaccharide through concentration and water extraction and alcohol precipitation. In vivo experiments prove that the inonotus obliquus extracellular and intracellular mixing crude polysaccharide can prolong the surviving time of mice hepatocellular carcinoma H22 and S180 ascites tumor mice, and can obviously improve the thymus gland index and spleen index of the mice. Furthermore, the inonotus obliquus extracellular and intracellular mixing crude polysaccharide can obviously improve secretion of IL-2, TNF-alpha cell factors in the blood serum of the tumor-bearing mice, and can be used for preparing new immunity intensifying preparation. The invention opens up a new way for development and utilization of medical fungus resources.

Description

technical field [0001] The invention relates to an intracellular and extracellular mixed crude polysaccharide of Inonotus obliquus with the effect of enhancing immunity. The crude polysaccharide has therapeutic effects in the aspects of tumor prevention and treatment, strengthening the body of the infirm, and low immune function, and belongs to the technical field of medicine. Background technique [0002] Inonotus obliquus, also known as Inonotus obliquus and Inonotus obliquus in China. Also known as Phaeoporus obliquus, Fuscoporia obliqua, Chaga, Black birch tinder (tuchwood), birch mushroom, Kabanoanoanatake, etc. It belongs to Basidiomycotina, Hymenomycetes, Aphyllophorales, Hymenoehaetaeeae. [0003] Morphology of Inonotus obliquus: Its fruiting bodies are tumor-like (sterile lumps), 25-40 cm in diameter, dark, with deep cracked surface, very hard, and brittle when dry. Spores broadly elliptic to ovate, smooth, with setae. The hyphae are two-lineage hyphae system wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08B37/00A61K31/715A61P37/04C12R1/645
Inventor 王琦王金玲潘景芝李玉
Owner 王琦
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