1340results about How to "Maintain biological activity" patented technology

A fibrillation-resistant insulin analogue may be a single-chain insulin analogue or a physiologically acceptable salt thereof, containing an insulin A chain sequence or an analogue thereof and an insulin B chain sequence or an analogue thereof connected by a polypeptide of 4-10 amino acids. The fibrillation-resistant insulin analogue preferably displays less than 1 percent fibrillation with incubation at 37° C. for at least 21 days. A single-chain insulin analogue displays greater in vitro insulin receptor binding than normal insulin while displaying less than or equal binding to IGFR than normal insulin. The fibrillation-resistant insulin may be used to treat a patient using an implantable or external insulin pump, due to its greater fibrillation resistance.

The invention relates to methods and materials that, for example, function to increase the barrier properties of containers including polymeric drug medication reservoirs and related containers such as infusion set tubing. Embodiments of the invention include aqueous container systems having containers coated with a composition selected to have one or more material properties including an ability to reduce the diffusion or permeation of compounds such as oxygen, carbon dioxide, and preservatives (e.g. phenol, benzyl alcohol and m-cresol) into or through a wall of the container.

The invention relates to a micro-ecological regulator, which belongs to the category of micro-ecological preparations. The product contains Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium adolesentis, lactobacillus acidophilus, fructo-oligosaccharides, galacto-oligosaccharides, vitamins and the like. A concrete production process comprises the following steps: 1. bacterial strains are respectively or jointly subjected to liquid deep high-density cultu, so as to obtain fermentation liquid; 2. after the fermentation liquid is centrifuged, bacterial sludge is collected, added to protective agents and made into freeze-dried powder; 3. the freeze-dried powder is added to microcapsule materials and made into microcapsules through air suspension treatment; and 4. the microcapsules are mixed with oligosaccharides, vitamins and the like and then are made into a synbiotics pharmaceutical composition. By directly replenishing human intestines with Bifidobacterium and lactobacillus acidophilus, and by simultaneously providing prebiotics for ensuring that beneficial bacteria after entering intestines can be activated rapidly and proliferate, the product adjusts and improves intestinal micro-ecosystem, so as to achieve the aims of regulating organism immunity, delaying senility, inhibiting tumors, regulating blood lipid and improving intestines and stomach. A preparation method of the product can solve the technical problem that the prior probiotics medicine is difficult to keep the stability of probiotics at normal temperature and unstable under acidic conditions, and provides a pharmaceutical composition which can be stably stored at normal temperature.

A combined rapid acting-long acting insulin formulation has been developed wherein the pH of the rapid acting insulin is decreased so that the long acting glargine remains soluble when they are mixed together. In the preferred embodiment, this injectable basal bolus insulin is administered before breakfast, provides adequate bolus insulin levels to cover the meal, does not produce hypoglycemia after the meal and provides adequate basal insulin for 24 hours. Lunch and dinner can be covered by two bolus injections of a fast acting, or a rapid acting or a very rapid acting insulin. As a result, a patient using intensive insulin therapy should only inject three, rather than four, times a day. Experiments have been performed to demonstrate the importance of the addition of specific acids to hexameric insulin to enhance speed and amount of absorption and preserve bioactivity following dissociation into the monomeric form by addition of a chelator such as EDTA. As shown by the examples, the preferred acids are aspartic, glutamic and citric acid. These are added in addition to a chelator, preferably ethylenediaminetetraacetic acid (EDTA). The results show that the citric acid formulation was more effective at dropping the blood glucose rapidly than the identical rapid acting formulation prepared with HCl in swine. Charge masking by the polyacid appears to be responsible for rapid insulin absorption. EDTA was not effective when used with adipic acid, oxalic acid or HCl at hastening the absorption of insulin. These results confirm the results seen in clinical subjects and patients with diabetes treated with the rapid acting insulin in combination with citric acid and EDTA.

The present invention relates to a method for extracting the purificatory cordycepin and the cordyceps amylase from the cordyceps militaris, belonging to the fields of medicines and health protection and chemical engineering. The cordyceps militaris powder is extracted and filtrated by a cooling seep method, the filter residue is mixed with ethanol, the supersonic wave assists to extract and offcenter, the supernatant has the processes of concentration and alcohol-precipitating, then the alcohol-precipitated supernatant has the processes of decompression, concentration and elution, the eluted liquid has the processes of concentration and crystallization and recrystallization using the n-butyl alcohol, at last the purificatory amylase is obtained after freezing and drying. The preparation progress of the present invention only uses water and ethanol as the solvent, thus reducing the pollution, the resin is capable of being reused a plurality of times with low cost. The present invention fully exploits the biological activity ingredients of the cordyceps militaris, realizes the coextraction of the cordycepin and the cordyceps amylase, guarantees the biological activities of the cordycepin and the cordyceps amylase, farthest increases the utilization rate of the raw material to the largest extent, reduces the manufacturing cost. The present invention is suitable for the large-scale industrialized manufacture.

The present invention provides a method of stably storing a protein, the method comprising applying a protein to be stored to a substrate which has been treated with a polyhydric compound and dried, wherein the amount of the polyhydric compound present in the substrate is sufficient to stabilise the protein, and wherein the substrate does not consist of glass. In one embodiment the protein to be stored is trypsin.

The invention provides a preparation method of high-purity collagen protein sponge, and relates to a preparation method of collagen protein sponge. The preparation method of the high-purity collagen protein sponge is used for solving the problems that the collagen protein sponge prepared by using a conventional method is long in production cycle and low in yield and purity and has poor hemostasis performance. The preparation method of the high-purity collagen protein sponge comprises the following steps: step one. pretreating fresh bovine heel tendons; step two. extracting collagen protein; step three. centrifuging; step four. salting out; step five. dissolving; step six. carrying out gradient dialysis; step seven. pre-freezing; step eight. carrying out freeze drying; and step nine. sterilizing. The final product prepared by using the method has a smooth and flat surface and relatively good hemostatic performance and is uniform in pore size distribution. The product has relatively high purity (the total amount of amino acids reaches 97.73%), an obvious hemostatic effect and no abnormal taste, is safe, non-toxic, high in yield and short in time; liquid is clear without impurities; the production cycle is shortened; the whole preparation process is carried out at a room temperature; the biological activity of the collagen protein is maintained; and the application of the high-purity collagen protein sponge in clinical is improved.

A freeze-dried high-temp. resistant protecting agent for virus-type biologic products for veterinary medicine is prepared from several components through proportionally mixing. It features that each of its components is sterilized separately. For the components which can be sterilized under high temp., they are dissolved in distilled water according to the proportion of formulation and are sterilized at 116 deg.C for 30-40 min; and for those which do not resist the high temp., they are also dissolved in distilled water according the proportion of formulation and are filtered with 0.22 micron pore diameter filter membrane to remove bacteria; and then the two parts are mixed to obtain the freeze-dried high-temp. resistant, protecting agent. The mentioned two parts are added to the virus antigen liquid according to a proportion of 1:1 and after packaging, they are freeze-dried. The product can be stored at 2-8 deg.C for 24 months.

A microneedle or microneedle device includes a microneedle body extending from a base to a penetrating tip formed from a silk fibroin based material, which is easy to fabricate and highly biocompatible. The microneedle device can include one or more microneedles mounted to a substrate. The silk fibroin can include active agents to be transported into or across biological barriers such as skin, tissue and cell membranes. The silk fibroin microneedles can be fully or partially biodegradable and/or bioerodible. The silk fibroin is highly stable, affords room temperature storage and is implantable. The silk fibroin structure can be modulated to control the rate of active agent delivery.

The invention provides a preparation method for high-activity lepidium meyenii extract. The preparation method is characterized by comprising the following steps of: crushing fresh or dry lepidium meyenii roots, and performing primary ultrasonic reinforced extraction, primary solid-liquid separation and primary low-temperature supernatant concentration under the conditions of certain liquid/solid ratio and 20 to 50 DEG C by taking water as a solvent; performing secondary ultrasonic reinforced extraction, secondary solid-liquid separation and secondary low-temperature supernatant concentration on filter residues under the conditions of certain liquid/solid ratio and 20 to 50 DEG C by using an ethanol-water solution; and mixing the two concentrated solutions, and drying the mixture at low temperature or quickly to obtain powdery lepidium meyenii extract. The preparation method has the characteristics of high speed, high efficiency, completeness of functional component extraction, capability of preventing the damage of heat-sensitive functional components of lepidium meyenii, high bioactivity of the extract, low energy consumption, low cost and the like. The lepidium meyenii extract prepared by the preparation method provided by the invention has the effects of eliminating fatigue, resisting pressure, improving immunity, alleviating climacteric syndromes, resisting prostatic hyperplasia, enhancing sexual function, and the like.

The present invention is directed stromal cell derived factor-1 peptides that have been mutated to make them resistant to digestion by the proteases dipeptidyl peptidase IV (DPPIV) and matrix metalloproteinase-2 (MMP-2) but which maintain the ability of native SDF-1 to attract T cells. The mutants may be attached to membranes formed by self-assembling peptides and then implanted at sites of tissue damage to help promote repair.

The invention relates to a separating, purifying and inspecting method of anthocyanin in blueberry wine dregs, which belongs to the technical field of functional active constituent extraction and purification. In the separating method, blueberry wine dregs or frozen blueberry wine dregs are ground into slurry or are freeze-dried and crushed into powder; and the slurry or the powder is separated in a warm extraction method in an acidified ethanol solution, and blueberry anthocyanin coarse extracting liquid is obtained through reduced pressure concentration. The blueberry anthocyanin coarse extracting liquid is purified and concentrated to prepare a blueberry anthocyanin concentrated solution. The concentrated solution is freeze-dried to obtain blueberry wine dreg anthocyanin freeze-dried powder. In the invention, the anthocyanin is separated out from waste produced by blueberry wine, which shows the research and development of simple, efficient, environment-friendly and low-cost production technology; and the content of the anthocyanin in the blueberry anthocyanin freeze-dried powder is 10-40%. The anthocyanin concentrated solution or coarse extracting liquid can be directly manufactured into oral liquid, tablets and other functional foods. The anthocyanin freeze-dried powder can be manufactured into food stain with an obvious antioxidation to be widely used in the fields of beverages, wines and the like.

The invention relates to a method for preparing fish scale collagen. The method comprises the following steps: fresh fish scales are taken as a material, undergo cleaning, drying and crushing, are soaked in NaOH solution for degreasing, and soaked in hydrochloric acid solution for deashing; the fresh fish scale is added with water of which the weight is 5 to 10 times of that of the fish scales, the pH value is adjusted to between 2 and 4 by acid solution, pepsin of which the weight accounting for the weight of the mixing solution is between 1 and 5 percent is added, the mixing solution undergoes enzymolysis at a low temperature for 4 to 10 hours, and enzyme is killed; enzymatic liquid is filtered twice to obtain extracting solutions, the extracting solutions are mixed, the mixing solution is decolored, sodium chloride of which the weight accounting for the weight of the mixing solution is between 5 and 15 percent is added into the mixing solution for overnight salting out, deposit is separated and collected, and acetic acid of which the weight accounting for the weight of the mixing solution is between 5 and 20 percent is added into the deposit for dissolution to obtain protein solution; the protein solution is dialyzed and desalted to obtain purified collagen liquid; and the collagen liquid is subjected to freeze-drying or spray-drying and micro-crushing to obtain solid particle. The method has the advantages of reasonable process, simple operation, safety, low cost, little loss of nutrient matters of the product, low ash content and high yield and purity of the collagen.

The invention discloses a biomarker preserving liquid. The biomarker preserving liquid comprises at least one protein stabilizer, at least one alkaline or neutral amino acid, at least one polyol or polymer, and citrate. The invention also discloses a fluorescent marker reagent prepared by using the preserving liquid, a preparation method of the reagent, and a use of the preserving liquid. The preserving liquid has a very good preserving effect on a fluorescent tracer-biomarker conjugate in the fluorescent marker reagent, can keep the activity and stability of a fluorescent tracer, the biomarker and the conjugate to make the fluorescent marker reagent form a liquid commercial reagent which can be directly used, so the preserving liquid is convenient to use; and the fluorescent marker reagent prepared by using the preserving liquid can tolerate short time high-temperature transportation, so the cost reduction is facilitated.

The invention discloses an immune base preparation method and an antigen or antibody immunoassay method. According to the immune base preparation method, a silicon slice is modified by using gold or silver nanoparticles, and antibodies/antigens are modified on the surfaces of the gold or silver nanoparticles, thereby being used for capturing antigens/antibodies to be assayed. According to the antigen or antibody immunoassay method, an immune base which is prepared by using the immune base preparation method and gold or silver nanoparticle immunoprobes is used, an immune base-antigen/antibody-immunoprobe three-layer sandwich structure is formed through an antigen-antibody immune complex reaction, and the assay on the antigens/antibodies to be assayed is realized in a manner that the characteristic dactylograms of Raman markers on the surfaces of the immunoprobes are assayed by using a surface-enhanced Raman scattering effect of the gold or silver nanoparticles. The method has the advantages that the sensitivity of the immunoassay can be greatly improved and the assay on high-flux multiple antigens/antibodies can be carried out.

The invention provides a collagen and a collagen polypeptide, wherein, the weight percentage of hydroxylysine is more than 1.3 percent. The invention also provides the preparation technology and the application of the collagen and the collagen polypeptide. The invention can make full use of inexpensive raw materials including the fish skin and fish scale of tilapias to produce products with high added value. The collagen polypeptide is used for treating arthritis with a definite drug effect, and the clinical dose is considerably less than other collagen polypeptides. The collagen polypeptide can solve the pollution problem of fish skin wastes, and at the same time avoid the risk of infectious diseases which can be caused by the collagen of land animals. The collagen and the collagen polypeptide have the advantages of considerable economic benefits, energy conservation and environmental protection, simple process equipment, low cost and easy industrialization.