160results about How to "Maintain biological activity" patented technology

The invention relates to the technical field of freeze-dried preparations, and provides exosome freeze-dried powder as well as a preparation method and application thereof. The exosome freeze-drying preparation is prepared by mixing an exosome solution and a freeze-drying protection solution and then freeze-drying the mixture, the concentration of the exosome solution in the mixed solution is 0.1-10% V / V, and the freeze-drying protection solution comprises 0.4-4% W / V of saccharides and 0.25-4% W / V of alcohols; the saccharide is trehalose, cane sugar or lactose; the alcohol is mannitol, sorbitol or a combination of mannitol and polyethylene glycol; and the solvent is sterilized water for injection or a PBS buffer solution. The exosome freeze-dried powder is stable in activity, small in irritation, good in safety and good in fibroblast proliferation promoting capacity, when the exosome freeze-dried powder is applied to supporting essence or masks in the medical beauty industry, the exosome activity of the exosome freeze-dried powder is still kept stable, and the application prospect is good.

The invention discloses a glucose response type insulin delivery system as well as a preparation method and application thereof, and belongs to the technical field of biological materials. The glucose response type insulin delivery system provided by the invention comprises a delivery carrier and a bioactive component encapsulated in the carrier, the delivery carrier is a metal polyphenol network formed by carrying out coordination reaction on epigallocatechin gallate and metal ions; the bioactive components comprise glucose oxidase, catalase and insulin. The glucose response type insulin delivery system provided by the invention can adjust the release of insulin according to the concentration of glucose in the surrounding environment, so that the blood glucose is maintained at a normal level.

The invention discloses a bioreactor process optimization system and method, and relates to the technical field of bioreactors. Comprising the following steps: collecting historical reaction process data of a bioreactor, and obtaining real-time reaction process data of the bioreactor; analyzing the historical reaction process data to obtain different influence results of different adjustment modes on the biological reaction process; establishing a cell culture model according to a corresponding relation obtained by different influence results of different adjustment modes on the biological reaction process; the real-time reaction process data is obtained, the biological culture process is influenced by adjusting the bioreactor, the specific adjustment mode is obtained through the real-time reaction process data and the required adjustment result, the required adjustment result can be obtained after the reaction in the adjustment mode obtained through backstepping, hysteresis is avoided, materials are saved, and the efficiency is improved. And the problem of poor subsequent biological reaction caused by excessive material addition, excessive design parameter adjustment and poor subsequent biological reaction due to hysteresis is also avoided.

The invention provides a hydroxytyrosol supramolecular compound and a preparation method and application thereof, and belongs to the technical field of cosmetics and biological medicine, the supramolecular compound comprises the following chemical components by mass: 0.01-10% of hydroxytyrosol, 2-45% of an amino acid compound, 2-20% of alpha hydroxy acid, 2-30% of urea, and 15-92% of water. The supramolecular compound can promote transdermal delivery of hydroxytyrosol and maintain the biological activity of hydroxytyrosol, shows high biocompatibility, and can be applied to product development in the antibacterial field, the regeneration field and the anti-aging field.

The invention belongs to the technical field of functional foods and health foods, and particularly relates to an anti-saccharification and anti-aging aronia melanocarpa fruit composition and a preparation method thereof. The composition is prepared from the following raw materials in parts by weight: 150 to 400 parts of aronia melanocarpa fruit fermentation liquor, 10 to 80 parts of lycium ruthenicum, 10 to 80 parts of ginseng, 0.5 to 2.5 parts of grape seed extract, 10 to 70 parts of collagen peptide, 0.5 to 4 parts of sodium hyaluronate, 0.1 to 0.8 part of resveratrol and 0.03 to 0.3 part of yeast extract. The content of type I collagen peptide in the skin is increased, the generation of in-vitro anti-glycation end products is inhibited, and the anti-glycation and anti-aging effects are achieved.

The invention discloses a biological filler for enhancing denitrification of an aerobic tank as well as preparation and application of the biological filler, the problem of weak activity of denitrifying bacteria is successfully solved by loading three layers of biological fillers, namely an inner layer-denitrifying bacteria, a middle layer-conducting layer and an outer layer-nitrifying bacteria, on the filler, and rapid formation of a biological membrane is realized. In addition, a metal conducting layer is additionally arranged in the middle to participate in electron transfer in the microbial denitrification process, and the biological membrane layers on the two sides achieve rapid formation of biological membranes so as to achieve conversion and removal of nitrogen. According to the method, the biological activity of the thalli can be kept, the rapid formation of a biological membrane is realized, and the separation of the thalli and refractory organic matters can be promoted, so that the toxic action on the thalli is reduced.

The invention relates to a ginseng, ganoderma lucidum and red date preparation with anti-oxidation and anti-aging effects and application thereof, and belongs to the field of health-care food, and the preparation comprises a ganoderma lucidum polysaccharide extract, a ginseng extract and a red date polyphenol extract. According to the method, the high-voltage pulsed electric field is combined with the Sevage method to extract the ganoderan, the extraction efficiency and purity can be improved while the biological activity of the ganoderan is maintained, and the method has the advantages of being short in time, low in cost, easy and convenient to operate and the like and is suitable for industrial production. Meanwhile, the synergistic effect of the components is achieved, the optimal anti-oxidation and anti-aging effects are achieved, and the prepared ginseng, lucid ganoderma and red date oral liquid preparation has the excellent anti-oxidation and anti-aging effects.

The invention provides poria cocos beta-glucan composite hydrogel. Poria cocos beta-glucan, dimethyl sulfoxide and pure water are used as raw materials; wherein the poria cocos beta-glucan and the dimethyl sulfoxide are mixed to form a gel polysaccharide / DMSO solution, and the gel polysaccharide / DMSO solution and the pure water are assembled into a supramolecular structure through an annealing technology. The invention also provides a preparation method of the tuckahoe beta-glucan composite hydrogel and application of the tuckahoe beta-glucan composite hydrogel in preparation of antibacterial, anti-inflammatory, antioxidant or skin defect repair materials. The tuckahoe beta-glucan composite hydrogel is good in mechanical property, can maintain the biological activity of a loaded drug to the maximum extent, and has application value in the aspects of antibiosis, anti-inflammation, antioxidation and skin defect repair.

The invention relates to a ginseng and ganoderma lucidum preparation with anti-oxidation and anti-aging effects and application of the ginseng and ganoderma lucidum preparation with the anti-oxidation and anti-aging effects, ginseng and ganoderma lucidum are used as main components, and the ginseng and ganoderma lucidum preparation with the anti-oxidation and anti-aging effects is obtained through a specific preparation technology.

The invention provides a collagenous fiber material as well as a preparation method and application thereof, relates to the field of organic high-molecular compound processing and also relates to the technical field of medical materials. According to the collagen fiber material and the preparation method thereof, polyhydric alcohol dispersion, saturated sodium chloride solution salting-out, dispersion liquid re-dispersion, self-assembly in a physiological simulation state and mutual compatibility of all the steps are achieved, the salt concentration and the pH value of all the steps are strictly controlled, collagen is prevented from being gathered and clustered due to rapid salting-out, fiber collagen is stably separated out under the condition that a cross-linking agent is not added, and the collagen fiber material has the advantages of being good in stability and good in stability. The obtained collagen has high mechanical strength and enzymolysis resistance, and can be used for medical filling, implantation and the like.

The invention discloses an induction culture medium for preparing human umbilical cord mesenchymal stem cell exosomes and application of the induction culture medium, and relates to the technical field of exosome culture. The additive is prepared from the following components: 0.8 to 1.5 percent of ITS, 0.03 to 0.05 percent of a luteolin extract, 0.04 to 0.06 percent of a herba taraxaci extract, 0.02 to 0.04 percent of a radix ginseng extract, 0.03 to 0.05 percent of resveratrol, 0.01 to 0.03 percent of vitamin C, 0.03 to 0.05 percent of a pimpinella thellungiana extract, 0.01 to 0.03 percent of a rhizoma dioscoreae extract and 0.01 to 0.02 percent of sodium citrate; the induction culture medium provided by the invention not only can avoid introduction of exogenous substances and reduce the pollution risk, but also can promote proliferation of stem cells and effectively induce synthesis and exosome secretion of a large number of human umbilical cord mesenchymal stem cells.

The invention belongs to the technical field of purification processes, and particularly relates to an enzymolysis extraction method of ginseng polysaccharide and application of the ginseng polysaccharide in food. The enzymolysis extraction method comprises the following steps: S1, drying a ginseng medicinal material, crushing, sieving with a 30-50-mesh sieve to obtain dry ginseng powder, adding distilled water, decocting for 2-4 hours, extracting for 2-3 times, combining decoction, concentrating, adding ethanol until the alcohol content of a system is greater than or equal to 80%, standing for 24 hours, centrifuging to obtain a precipitate, and drying the precipitate; s2, mixing the dried precipitate with distilled water, and adding enzyme for enzymolysis extraction to obtain an extracting solution; s3, concentrating the extracting solution until the volume of the extracting solution is 20-25% of that of the extracting solution, centrifuging to remove precipitates, dialyzing, and concentrating and drying to obtain crude ginseng polysaccharide; and S4, dissolving the crude ginseng polysaccharide in distilled water for separation and purification. The variety, adding sequence and conditions of the enzymes are optimized, the extraction rate and purity of the ginseng polysaccharide are improved, and meanwhile the biological activity of the ginseng polysaccharide is kept. The extracted ginseng polysaccharide can be widely applied to various foods.

The invention relates to a freeze-drying treatment process suitable for a bioactive coating. The freeze-drying treatment process comprises the following factors: coating freeze-drying pretreatment: preparing an excipient and an auxiliary material solution; in the pre-freezing part, the pre-freezing temperature, the shelf temperature before feeding and the pre-freezing time are determined; the freeze-drying time part comprises the fastest temperature point of one-time sublimation drying, the drying time of a high-speed section of one-time sublimation, the drying time of a low-speed section of one-time sublimation and the desorption drying time. And the vacuum degree part is one-time sublimation air doping amount. The method is simple, the technology is mature and feasible, the freeze-dried coating product is regular in appearance and extremely low in water content, the defects of an existing bioactive coating preparation technology are overcome, the actual application requirement is met, the drying technology in most bioactive coating preparation processes can be replaced, the biological activity of the coating is guaranteed, the service life of the coating is prolonged, and the application prospect is wide. The industrial application of the coating is facilitated, and powerful support is provided for related research.

The present invention discloses a mechanically reinforced 3D printed calcium phosphate ceramic scaffold and a preparation method thereof. The present invention also discloses the use of the above-mentioned mechanically reinforced 3D printed calcium phosphate ceramic scaffold in the preparation of bone defect repair materials. Different from the traditional reinforcement method of adding second-phase whiskers, the present invention introduces metal salts that induce the growth of calcium phosphate grains into the photocurable 3D printing slurry, effectively avoiding the influence of the added whiskers on the fluidity of the printing slurry, and then performs normal printing and molding, and finally regulates the directional growth of calcium phosphate grains in the sintering process to form a mechanically reinforced whisker structure, which significantly improves the mechanical properties of the material on the basis of ensuring that calcium phosphate can be 3D printed and customized, biologically active, and safe; at the same time, the metal salt that regulates the growth of calcium phosphate grains also has biological functions, which can further enhance the biological activity of calcium phosphate ceramics.

The present invention discloses a mask-type respiratory pathogen sampling device, comprising a mask, an air supply pipe, a collecting pipe, a pipe cover, an air dispersion coil, an exhaust pipe and a cold storage box, wherein the mask is provided with a one-way valve air inlet and an air outlet; the collecting pipe is detachably provided with a pipe cover, and an air dispersion coil and an exhaust pipe are inserted on the pipe cover, and the air dispersion coil runs through the bottom of the collecting pipe; one end of the air supply pipe is connected to the air outlet of the mask, and the other end is connected to the pipe mouth of the air dispersion coil; the collecting pipe is filled with pathogen collection and preservation liquid; the collecting pipe can be adaptively placed in the cold storage box. The innovative point of the present invention is that the mask intercepts the gas exhaled by the sampled person, introduces it into the air dispersion coil immersed in the sampling preservation liquid, and then exhausts the gas through the micropores on the spiral air dispersion coil, dissolving the pathogens carried in the bubbles in the pathogen collection and preservation liquid, so as to achieve the purpose of efficiently collecting respiratory pathogens.

The invention discloses an extraction method of American ginseng glycoprotein, an obtained product and application, American ginseng is used as a main raw material, a natural eutectic solvent aqueous solution is used as a solvent, warm immersion stirring, ultrasonic and microwave extraction are performed in sequence to obtain an extracting solution, then the extracting solution is subjected to subsequent operations such as alcohol precipitation and free protein removal, and the American ginseng glycoprotein is obtained. The American ginseng glycoprotein is obtained. The American ginseng glycoprotein extraction method is simple in process, high in safety, high in yield and purity and suitable for industrial production, and the obtained American ginseng glycoprotein has liver protection / liver protection and antioxidant activity, can be prepared into drugs, health care products and food, provides clinical guidance and medication basis for patients with liver diseases, further improves the life quality of the patients and has a good clinical application prospect. And the method has good application value.

The invention discloses a maleic anhydride sewage treatment process and a sewage treatment system. The maleic anhydride sewage treatment process comprises the following steps: S1, carrying out buffer treatment on maleic anhydride sewage; s2, carrying out demulsification and multi-dimensional electro-catalysis pretreatment on the maleic anhydride sewage treated in the step S1; s3, carrying out anaerobic reaction treatment on the maleic anhydride sewage treated in the step S2; s4, performing neutral catalytic oxidation and aerobic treatment on the maleic anhydride sewage treated in the step S3; s5, performing electro-catalysis treatment on the maleic anhydride sewage treated in the step S4 to obtain sewage meeting the standard; the technical problems that in the prior art, the maleic anhydride sewage is high in pollutant concentration, low in biochemical treatment degradation speed and low in efficiency are effectively solved by removing specific pollutants such as DBP and DOP in the maleic anhydride sewage and adopting a method of demulsification, multi-dimensional electro-catalysis, efficient anaerobic treatment, multi-stage aerobic treatment, catalytic oxidation and electro-catalysis.

The invention provides a preparation method of a nano microemulsion loaded with rare saponin in red ginseng extract and American ginseng extract, which comprises the following specific steps: extracting the rare saponin in the red ginseng extract and American ginseng extract, preparing a nano liposome loaded with the rare saponin, modifying with modified sesbania gum and preparing the nano microemulsion loaded with the rare saponin. Hydrophilic and hydrophobic rare ginsenoside are simultaneously embedded in the nano-liposome, in addition, the nano-liposome is modified by adopting modified sesbania gum, and the nano-liposome is endowed with an outer membrane structure by adopting the sesbania gum modified by genipin and feruloyl oligosaccharide, so that the stability of the nano-liposome can be improved, the biological activity of the rare ginsenoside is ensured, and the bioavailability of the rare ginsenoside is improved. In addition, the rare ginsenoside can be released in an organism in a targeted mode, genipin and feruloyl in the modified sesbania gum interact with phospholipid molecules in the nano-liposome in the intestinal environment, decomposition of the nano-liposome is further accelerated, rapid release of the rare ginsenoside is achieved, and a new method is provided for research and utilization of the rare ginsenoside.

The invention relates to the technical field of biological medicine, and particularly discloses an SIRT6 outer vesicle freeze-drying protective agent, SIRT6 outer vesicle freeze-dried powder and a preparation method and application thereof. The SIRT6 external vesicle freeze-drying protective agent provided by the invention is prepared from the following components: 0.8 to 1.5 g / 100 mL of polyvinyl alcohol, 0.5 to 0.8 g / 100 mL of dextran, 5.5 to 6 g / 100 mL of trehalose, 2.5 to 4 g / 100 mL of mannitol, 0.35 to 0.4 g / 100 mL of vitamin C, 0.15 to 0.3 g / 100 mL of hydroxyectoine, 0.8 to 1.2 g / 100 mL of superoxide dismutase and a solvent which is a phosphate buffer solution. The invention further provides freeze-dried powder of the SIRT6 external vesicles and a preparation method of the freeze-dried powder. According to the SIRT6 outer vesicle freeze-drying protective agent, the structural damage of freeze-drying to the SIRT6 outer vesicle can be reduced, and the structural integrity and functionality of the SIRT6 outer vesicle are ensured.

The invention discloses a preparation method of a fermented soybean extract-containing composition for controlling blood sugar of type II diabetes people, and particularly relates to the technical field of blood sugar control, and the preparation method comprises the following steps: preparing raw materials, preparing a fermented soybean extract, mixing the materials, applying a low-temperature crushing technology, and detecting alpha-glucosidase inhibitory activity of the fermented soybean extract. A novel packaging material is adopted for packaging. Through the synergistic effect of various natural extracts, such as the fermented soybean extract for inhibiting digestive enzymes, the white kidney bean extract and the mulberry leaf extract for regulating blood sugar and the like, the blood sugar level of a patient is effectively reduced, and the formation mechanism of converting starch food into saccharides and the formation mechanism of fat can be quickly and effectively controlled; the traditional Chinese medicine composition has the effects of quickly regulating blood sugar, controlling body weight, improving organism immunity, regulating blood fat and the like, and reduces the pain of various adverse reactions caused by medicine taking of type II diabetes patients, and the preparation method brings new hope to the type II diabetes patients and is expected to play an important role in diabetes prevention and treatment.

The invention provides a method for increasing the dissolution rate of beta-1, 3 / alpha-1, 3-glucan, which comprises the following steps: a, taking beta-1, 3 / alpha-1, 3-glucan as a raw material, spraying an adhesive into the beta-1, 3 / alpha-1, 3-glucan, uniformly mixing, wetting, and preparing a soft material; b, sieving the soft material prepared in the step a through a 30-40-mesh sieve, and granulating; and c, drying to obtain the beta-1, 3 / alpha-1, 3-glucan particles. According to the method disclosed by the invention, the optimal particle size range is screened and determined by accurately controlling the preparation process of the glucan particles, so that the rapid dissolution of the glucan particle preparation is realized. The method not only maintains the biological activity and purity of the glucan, but also greatly improves the efficiency and effect of the glucan in practical application, meets the requirement of industrial production for rapid dissolution, and has wide market prospects and application values.

The invention relates to the technical field of biological medicines, in particular to a preparation method of protein cross-linked nano affinity microspheres, which comprises the following steps: preparing carboxylated silicon dioxide nano microspheres with the particle size of 500nm by adopting a sol-gel method, mixing tetraethoxysilane and ammonia water according to the volume ratio of 1: (3 + / -1), reacting in an ethanol solution at 40 DEG C for 6 hours, filtering, washing, and drying to obtain the protein cross-linked nano affinity microspheres. And centrifuging to obtain a microsphere precipitate, washing with deionized water for three times to finally obtain a nano microsphere suspension of which the surface is rich in carboxyl, and controlling the solid content to be 10 + / -0.5 mg / mL. The capturing efficiency of the finally prepared protein cross-linked nano affinity microspheres for exosomes reaches 95.0%, the binding capacity reaches 28.5 mu g / mg, the purity of the extracted exosomes is higher than that of an ultracentrifugation method, the biological activity and structural integrity of animal cell exosomes and plant cell exosomes are completely kept, and the protein cross-linked nano affinity microspheres are suitable for large-scale industrial production. The problems that in a traditional exosome extraction method, the recovery rate is low, impurity pollution is serious, exosome activity is damaged, and the application range is limited are solved.

The invention discloses a generic tumor antigen antibody A11 and application thereof. The amino acid sequence of the CDR1 of the generic tumor antigen antibody A11 is as shown in SEQ ID NO. 1; the amino acid sequence of the CDR2 of the generic tumor antigen antibody A11 is as shown in SEQ ID NO. 2; and the amino acid sequence of the CDR3 of the generic tumor antigen antibody A11 is as shown in SEQ ID NO.3. The generic tumor antigen antibody A11 has strong affinity, a chimeric antigen receptor T cell taking the single-domain antibody as an antigen binding domain has strong killing ability to tumor cells, and the antibody can enhance the tumor cell killing ability of NK cells through ADCC effect.

The invention relates to bioglass powder containing nano titanium nitride as well as a preparation method and application of the bioglass powder. The bioglass comprises the following components in percentage by mass: 75-95% of bioglass powder and 5-25% of nano titanium nitride powder, the melting process of the bioglass containing nano titanium nitride comprises the following conditions: the temperature is 650-800 DEG C, and the time is 10-15 minutes; the photo-thermal treatment method comprises the step of applying near-infrared light to the nano titanium nitride-containing bioglass powder to regulate and control release of active ingredients in the bioglass, and can also comprise the step of inducing generation of hydroxyapatite on the surface of the bioglass. Compared with the prior art, the bioglass not only has a remarkable photothermal effect, but also has the advantages that the nano titanium nitride powder is not easy to lose and the photothermal performance of the bioglass containing the nano titanium nitride is kept good in an experiment of simulating implantation into a human body environment; the release of active ingredients in the bioglass and the generation of hydroxyapatite on the surface of the bioglass are regulated and controlled by applying near-infrared light, so that controllable and sustainable repair of bone tissues is realized. The technology can be applied to the fields of bone repair, regeneration and the like in bone tissue engineering.

The invention discloses a plant whitening and sunscreen composition as well as a preparation method and application thereof, and relates to the technical field of cosmetics. The invention provides a plant whitening and sunscreen composition. The plant whitening and sunscreen composition is prepared from the following components in parts by weight: 0.5 to 5 parts of P / O / W composite coating body, 0.1 to 3 parts of bakuchiol and 0.1 to 3 parts of thyme extract. According to the invention, pterostilbene and verbascoside are stabilized in a P / O / W pickering multiple emulsion system to prepare a P / O / W composite inclusion body, and the P / O / W composite inclusion body is mixed with the bakuchiol and thyme extract in specific parts by weight, so that on one hand, safe and efficient whitening can be realized by repairing, inhibiting melanin generation, inhibiting melanin transport and promoting melanin autophagy multi-channel synergistic interaction; on the other hand, whitening and sunscreen can be safely and efficiently achieved, the effect of external protection and internal whitening is achieved, the defensive barrier of the skin can be synergistically reinforced, and the problems of dark skin of sensitive skin and the like are solved.

The invention discloses a method for detecting NR2F1 antigen and a reaction pool thereof, belongs to the field of biotechnology, solves the problems of high detection cost of existing detection methods, and comprises the following steps: step A: preparing a second NR2F1 antibody solution modified by glucose oxidase; step B: preparing an electrode paper sheet of a working electrode modified by a first NR2F1 antibody; step C: inserting the electrode paper sheet in step B into the reaction pool, dripping NR2F1 antigen extract into the reaction pool, dripping the second NR2F1 antibody solution modified by glucose oxidase in step A, reacting for 1 to 3 minutes, dripping glucose solution, reacting for 20 to 40 seconds, and detecting the current; step D: dripping NR2F1 antigen extracts of different concentrations and repeating step C, and making a standard curve according to the relationship between the antigen concentration and the current value; step E: taking a sample to be tested for detection, and obtaining the NR2F1 antigen concentration value in the sample to be tested. The invention has the advantages of portable detection and low cost.

The invention discloses a constant-temperature wall-broken microwave plant extract and a production process thereof, relates to the technical field of extraction, and solves the technical problem of low activity during extraction of effective components of a product in the existing technical scheme. The constant-temperature wall-broken microwave plant extract is prepared from the following raw materials: 15-25 parts of passion fruits, 40-60 parts of grape seeds, 8-12 parts of lucid ganoderma powder and 15-25 parts of citrus peel powder. The preparation method comprises the following steps: S1, selecting passion fruits, grape seeds, lucid ganoderma and orange peels, and cleaning, disinfecting and sterilizing the passion fruits, the grape seeds, the lucid ganoderma and the orange peels; s2, inspecting and removing pollutants and impurities in the raw materials; s3, drying the cleaned raw materials, and grinding the dried raw materials into fine powder by using a grinder; according to the method, loss of active ingredients in traditional high-temperature extraction is avoided, nutritional ingredients and biological activity in plant raw materials are effectively kept, effective ingredients in plants can be efficiently extracted within a short time, and the production cycle is shortened; impurities and residues can be effectively removed, effective components can be reserved, degradation of heat-sensitive components is effectively prevented, and the nutritive value and natural fragrance of the raw materials are kept.

The application discloses a nano modification and high-density coating process for the surface of an enzyme-linked immunosorbent plate. The composition comprises an interface self-assembly bottom layer solution containing tannic acid and branched polyethyleneimine, and a dynamic gating functionalization buffer containing sodium tetraborate, D-mannitol and trehalose. The process first constructs a supramolecular bottom layer on a polystyrene surface, and then forms a boronic acid-mannitol gating interface without washing and drying. After the addition of an antibody, a competitive displacement reaction occurs between the Fc segment sugar chain and the boronic acid, the mannitol is displaced, and the directional high-density fixation of the antibody is realized. The application solves the problems of random direction and weak binding force of the antibody in the traditional physical adsorption, significantly improves the antibody loading capacity and detection sensitivity of the enzyme-labeled plate, and has good storage stability.

The invention provides application of a hericium erinaceus composite extract in preparation of a medicine for treating alcoholic liver injury, and relates to the technical field of the medicine for treating the alcoholic liver injury. The hericium erinaceus composite extract is composed of a hericium erinaceus petroleum ether extract, a hericium erinaceus ethyl acetate extract and a hericium erinaceus water extract. The hericium erinaceus composite extract is prepared from hericium erinaceus as a raw material through the process steps of ethanol extraction, petroleum extraction, ethyl acetate extraction, filter residue enzymolysis fermentation, water extraction, drying, mixing and the like. Through multi-step extraction and enzymolysis fermentation processes, the purity and activity of the extract are improved, the effects of various active ingredients are integrated, the overall curative effect of the medicine is enhanced, and the obtained composite extract can be used for preparing the medicine for treating the alcoholic liver injury and has a wide application prospect.

The invention discloses a construction method and application of a chlamydomonas reinhardtii source high-sweetness induced protein variant engineering algal strain. The preparation method comprises the following steps: firstly, modifying and optimizing protein coding sequences of sweet taste induction proteins Miraculin and Neoculin, so as to obtain optimized gene sequences of CrMiraculin and CrNeoculin; the method comprises the following steps: firstly, preparing a Chlamydomonas reinhardtii strain, then cloning the Chlamydomonas reinhardtii strain into an expression vector pGM6, constructing a recombinant expression vector, introducing the recombinant expression vector into the Chlamydomonas reinhardtii wild strain, and screening by using a paromomycin resistance marker carried by the vector to obtain a transformed strain. The obtained engineering algal strain can stably inherit and efficiently express the target sweet induced protein, and the chlamydomonas reinhardtii algal powder rich in the CrMiraculin and CrNeoculin sweet induced protein is obtained by performing fermentation culture on the engineering algal strain. Finally, through protein purification and mixing with acidic substances, sour taste can be induced to be converted into sweet taste, so that the method has great application potential in the fields of foods, seasonings or health-care products.