18results about How to "Maintain biological activity" patented technology

The invention provides a hydroxytyrosol supramolecular compound and a preparation method and application thereof, and belongs to the technical field of cosmetics and biological medicine, the supramolecular compound comprises the following chemical components by mass: 0.01-10% of hydroxytyrosol, 2-45% of an amino acid compound, 2-20% of alpha hydroxy acid, 2-30% of urea, and 15-92% of water. The supramolecular compound can promote transdermal delivery of hydroxytyrosol and maintain the biological activity of hydroxytyrosol, shows high biocompatibility, and can be applied to product development in the antibacterial field, the regeneration field and the anti-aging field.

The invention relates to the technical field of biological medicines, in particular to a preparation method of protein cross-linked nano affinity microspheres, which comprises the following steps: preparing carboxylated silicon dioxide nano microspheres with the particle size of 500nm by adopting a sol-gel method, mixing tetraethoxysilane and ammonia water according to the volume ratio of 1: (3 + / -1), reacting in an ethanol solution at 40 DEG C for 6 hours, filtering, washing, and drying to obtain the protein cross-linked nano affinity microspheres. And centrifuging to obtain a microsphere precipitate, washing with deionized water for three times to finally obtain a nano microsphere suspension of which the surface is rich in carboxyl, and controlling the solid content to be 10 + / -0.5 mg / mL. The capturing efficiency of the finally prepared protein cross-linked nano affinity microspheres for exosomes reaches 95.0%, the binding capacity reaches 28.5 mu g / mg, the purity of the extracted exosomes is higher than that of an ultracentrifugation method, the biological activity and structural integrity of animal cell exosomes and plant cell exosomes are completely kept, and the protein cross-linked nano affinity microspheres are suitable for large-scale industrial production. The problems that in a traditional exosome extraction method, the recovery rate is low, impurity pollution is serious, exosome activity is damaged, and the application range is limited are solved.

The application discloses a nano modification and high-density coating process for the surface of an enzyme-linked immunosorbent plate. The composition comprises an interface self-assembly bottom layer solution containing tannic acid and branched polyethyleneimine, and a dynamic gating functionalization buffer containing sodium tetraborate, D-mannitol and trehalose. The process first constructs a supramolecular bottom layer on a polystyrene surface, and then forms a boronic acid-mannitol gating interface without washing and drying. After the addition of an antibody, a competitive displacement reaction occurs between the Fc segment sugar chain and the boronic acid, the mannitol is displaced, and the directional high-density fixation of the antibody is realized. The application solves the problems of random direction and weak binding force of the antibody in the traditional physical adsorption, significantly improves the antibody loading capacity and detection sensitivity of the enzyme-labeled plate, and has good storage stability.

The invention discloses a construction method and application of a chlamydomonas reinhardtii source high-sweetness induced protein variant engineering algal strain. The preparation method comprises the following steps: firstly, modifying and optimizing protein coding sequences of sweet taste induction proteins Miraculin and Neoculin, so as to obtain optimized gene sequences of CrMiraculin and CrNeoculin; the method comprises the following steps: firstly, preparing a Chlamydomonas reinhardtii strain, then cloning the Chlamydomonas reinhardtii strain into an expression vector pGM6, constructing a recombinant expression vector, introducing the recombinant expression vector into the Chlamydomonas reinhardtii wild strain, and screening by using a paromomycin resistance marker carried by the vector to obtain a transformed strain. The obtained engineering algal strain can stably inherit and efficiently express the target sweet induced protein, and the chlamydomonas reinhardtii algal powder rich in the CrMiraculin and CrNeoculin sweet induced protein is obtained by performing fermentation culture on the engineering algal strain. Finally, through protein purification and mixing with acidic substances, sour taste can be induced to be converted into sweet taste, so that the method has great application potential in the fields of foods, seasonings or health-care products.

The invention provides an extract with high saikoside B2 content, a preparation method and application of the extract, and a combined medicine containing the extract, and belongs to the technical field of natural medicine preparation. The method comprises the following steps: efficiently extracting saikoside A and saikoside D in a radix bupleuri raw material by using an alkaline ethanol solution, further realizing efficient resolution of saikoside A and saikoside D by using macroporous adsorption resin, and quantitatively converting saikoside D into saikoside B2 by using a chemical method, thereby obtaining the high-content effective part of saikoside B2. The high-content effective part of saikoside B2 only contains a small amount of saikoside A except the main component saikoside B2, so that the toxicity is greatly reduced while the biological activity of the saikoside B2 is ensured. The high-content effective part of saikosaponin B2 can be used for preparing medicines for treating and preventing gout, rheumatoid arthritis, acute enteritis, skin light injury, skin inflammation and other diseases, can be independently used, and can also be combined with other medicines for use.

The application provides an edible active coating for preventing dry consumption under an ultralow temperature condition and a preparation method, and belongs to the technical field of active packaging. The application combines double-layer coatings, inhibits free radical oxidation generated by freezing through an inner antioxidant, and effectively locks water and blocks external oxygen permeation through the synergistic effect of an outer high-barrier structure, thereby significantly improving the freezing preservation effect of food, and the raw materials are natural and safe, the preparation method is reasonable, and the application has good practical application value. The application solves the problems of poor effects of edible freezing protection coatings suitable for extremely cold environments in the prior art.

The utility model relates to the field of hydrogen sulfide removal towers, in particular to a biological treatment mechanism in a hydrogen sulfide removal tower, which comprises a mounting bracket I, a mounting bracket II arranged on the lower side of the mounting bracket I, and a biological filler cylinder I arranged on the upper side of the mounting bracket I, a second biological filler cylinder corresponding to the first biological filler cylinder is arranged on the upper side of the second mounting support, biological filler layers are arranged in the first biological filler cylinder and the second biological filler cylinder, a liquid collecting tank is arranged on the upper side of the second biological filler cylinder, and a flow guide cover is fixedly mounted at the upper end of the liquid collecting tank; oxygen bottles are arranged on the outer sides of the first biological filler cylinder and the second biological filler cylinder, and oxygen supply main pipes are detachably mounted at the upper ends of the oxygen bottles; by arranging the oxygen bottle, the oxygen supply pipeline and the air outlet cover, when the biological activity is low, oxygen in the oxygen bottle can be conveyed to the air outlet cover through the oxygen supply pipeline and then discharged from the upper side and the lower side of the air outlet cover, and oxygen can be supplied to the biological filler layer.

This utility model discloses a cooling device for a low-speed grinding mill, including a grinding mill body. The upper end of the grinding mill body has a feed inlet connected to the grinding mill body. A pneumatic motor is fixed to the upper end of the grinding mill body. A cold air inlet is opened on the grinding mill body. A circulating water outlet and a circulating water inlet are opened on the right side of the grinding mill body. A solenoid valve is installed at the lower outlet of the grinding mill body. Inside the grinding mill body is a transmission rod connected to the pneumatic motor. The lower end of the transmission rod is connected to a grinding block. The lower end of the grinding block has a grinding disc. Inside the grinding block is an umbrella-shaped cooling chamber connected to the cold air inlet. A cooling cavity is opened inside the grinding mill body. The cold air inlet transmits the external low-temperature medium to the collar, and the air inlet transmits the low-temperature medium to the cooling chamber, cooling the grinding block as a whole and preventing the grinding block and grinding disc from overheating during the grinding of soapberry rice, which could cause the soapberry rice to stick together.

This invention provides an atomization-recirculation bioaerosol sampler, comprising: an aerosol mixing system, an aerosol separation system, an atomizing liquid replenishment system, a recirculation liquid collection system, an atomizing liquid level control system, a mist volume adjustment system, and an air intake flow rate adjustment system. The combined action of the aerosol mixing system and the aerosol separation system enables the capture of aerosols of different particle sizes in the air, while the aerosol separation system simultaneously recovers suspended droplets. The atomizing liquid replenishment system and the recirculation liquid collection system effectively ensure the stability of the sampling process. The atomizing liquid level control system allows the sampler to meet the requirements of long-term automatic sampling. The air intake flow rate adjustment system and the mist volume adjustment system allow for manual adjustment of key operating parameters of the sampler, meeting the adjustment needs of sampling conditions under special circumstances. This invention can reduce the damage to microbial cells caused by shear stress generated by impact during sampling, maintain the biological activity of the sample, and improve sampling efficiency.

The application discloses a kind of functionalized magnetic beads, which are magnetic microspheres formed by wrapping magnetic beads with bifunctional nucleic acid, and can specifically bind mRNA.A fungal mRNA extraction kit composed of functionalized magnetic beads includes grinding material, lysing solution I, lysing solution II, magnetic bead binding solution, washing solution I, washing solution II and eluent.A mRNA extraction method includes sequentially adding grinding material, lysing solution I, lysing solution II, magnetic bead binding solution, washing solution I, washing solution II and eluent after sample pretreatment to obtain mRNA product.The application breaks through the traditional mRNA extraction method, obtains high-quality mRNA, and has the advantages of simple operation, safety, high sensitivity, good reproducibility and low cost.The mRNA obtained by the application can provide high-quality templates for RT-PCR detection, improve the specificity and sensitivity of detection, and has wide application prospects in the fields of clinical diagnosis of diseases, judicial identification, food safety detection, microbial detection and molecular biology.

The present application relates to the technical field of deep processing of deer products, and discloses an enzyme-assisted high-activity ingredient extraction process of deer horn powder, which comprises the following steps: raw material pretreatment, composite enzymolysis, preparation of extraction liquid, ultrasonic-assisted extraction, separation and primary purification, adsorption purification, concentration and drying; the compact keratinization matrix structure of the deer horn powder is destroyed through defatting and decalcification treatment in the pretreatment step, which creates conditions for the release of subsequent active ingredients; the step of composite enzymolysis adopts step-by-step enzymolysis treatment, and the papain, acid protease, trypsin and cellulase are used for step-by-step enzymolysis in a controllable temperature oscillation reactor, which can hydrolyze the complex protein-polysaccharide composite structure in the deer horn, promote the dissolution of the internal high-value components, the multi-enzyme synergistic effect overcomes the limitations of single enzyme action, and thus the extraction sufficiency of the target ingredients and the breadth of the product spectrum are improved.

The invention discloses an active peptide capable of directionally inducing IPSCS cracking, a preparation method of the active peptide and application of the active peptide in a preparation for repairing liver injury. Experiments prove that the active peptide provided by the invention has a remarkable protective effect on a liver injury model induced by cyclophosphamide and shows a definite anti-oxidative stress effect. The result shows that a key path of chemotherapy injury can be intervened from the source by simulating or enhancing an endogenous protection mechanism of the liver. Compared with the existing exogenous synthetic drugs or single-component natural products, the product provided by the invention has the potential advantages of more comprehensive action mechanism and better biogenic property. Meanwhile, the extract is derived from directionally differentiated functional cells, and a brand new thought and material basis are provided for developing a novel chemotherapy liver protective agent which does not affect the chemotherapy curative effect and has good biocompatibility.

This utility model discloses a cryogenic operation platform for a liquid nitrogen tank, including a mounting plate. A through hole is provided in the middle of the upper surface of the mounting plate, and a threaded rod is installed inside the through hole. A placement plate is installed at the lower end of the threaded rod, and a threaded hole is provided in the middle of the placement plate. The lower end of the threaded rod is screwed into the threaded hole. Guide rods are provided at both ends of the upper surface of the mounting plate, passing through the mounting plate and the placement plate. This platform allows biological samples to be uniformly placed at the upper part of the liquid nitrogen tank. The low temperature inside the liquid nitrogen tank keeps the frozen biological samples or cells awaiting release at a cryogenic state. After all the required biological samples are removed, they are uniformly taken out of the liquid nitrogen tank and placed in a transport box. The cryogenic environment inside the liquid nitrogen tank keeps the frozen biological samples or cells awaiting storage at a cryogenic state, shortening the time the biological samples are exposed to air and reducing the impact on their activity. Furthermore, the platform can be flexibly adjusted according to the position of the biological samples inside the liquid nitrogen tank, facilitating the removal or storage of biological samples in different locations.

The utility model discloses a constant temperature storage device for ram semen, and relates to the technical field of frozen semen constant temperature equipment, including the heat preservation box, adjustable support leg and the universal wheel of rotation installation are fixedly installed in the bottom of heat preservation box, the heat preservation cover plate is fixedly sealed and installed at the top of heat preservation box, and a plurality of mounting hole slots are formed in the heat preservation cover plate, the mounting hole slot is used for fixedly installing the storage mechanism, and the storage mechanism is used for zoning isolation ram semen, the compression spring is fixedly arranged on the heat preservation box bottom plate one end, and the other end is connected with the storage mechanism, use this semen storage device, through the storage mechanism, make multiple thumb tube all be in the same low temperature environment, form stable low temperature field, avoid the different storage environment of each thumb tube because of temperature difference, simultaneously, when taking out a thumb tube, avoid the large loss of liquid nitrogen and cause the temperature of heat preservation box to produce greater fluctuation.

This invention discloses a pre-set scale spherical grinding minimally invasive bone harvesting device and its operation method, including a channel fixation module, a fixation tube, an outer sheath, an inner drive shaft, a rotation adjustment mechanism, and a drive device. The fixation tube is fixedly connected to the distal end of the channel fixation module. The proximal end of the outer sheath is rotatably mounted on the channel fixation module. The inner drive shaft is rotatably mounted in the distal end of the channel fixation module. The rotation adjustment mechanism is located at the proximal end of the inner drive shaft. A mesh cage is provided at the distal end of the outer sheath. A traction wire is connected between the rotation adjustment mechanism and the mesh cage to pull the mesh cage to expand to the pre-set bone harvesting diameter. The drive device drives the inner drive shaft to rotate the outer sheath, causing the mesh cage to perform spherical grinding of the bone. This invention can pre-determine the bone harvesting diameter through a pre-set scale, accurately control the bone harvesting volume, eliminate the need for repeated adjustments to the grinding range during surgery, and simplify the operation process.

This invention relates to a three-dimensional porous bioactive scaffold, a vascularized bone organoid, its preparation method, and its applications, belonging to the field of tissue engineering technology. The bioactive scaffold includes a scaffold body, a Piezo1 channel agonist loaded on the scaffold body, and an Nrf2 agonist and / or Nrf2 expression vector loaded on the scaffold body; the scaffold body is a 3D-printed PMBG / β-TCP composite porous scaffold. The vascularized bone organoid uses the three-dimensional porous bioactive scaffold as a supporting framework and also includes cellular components, including co-cultured bone marrow mesenchymal stem cells and endothelial cells. Compared with existing technologies, this invention successfully constructs a novel vascularized bone organoid capable of targeting the Piezo1-Nrf2 signaling axis and possessing anti-endothelial aging and mechanical enhancement functions.

The invention discloses a medical glucan skin repair dressing, which comprises Ectoine, beta-glucan, sodium hyaluronate, trehalose, sodium carboxymethyl cellulose and the like, and is a sterile liquid dressing without glycerol and traditional chemical preservatives. The dressing has appropriate viscosity, excellent film-forming property and flexibility, can quickly form a transparent, breathable and flexible protective film after being applied to a wound surface, can be used for nursing skin barrier injury, low sensitization and healing promotion of superficial wound surfaces, energy equipment postoperative wound surfaces and the like, and is convenient to use.

The application relates to a biological 3D printing cartilage-fiber-epithelium integrated tissue engineering trachea and a construction method and application thereof. First, cartilage specific biological ink is prepared by wrapping cartilage cells with cartilage tissue specific hydrogel; fiber specific biological ink is prepared by wrapping fibroblasts with fiber tissue specific hydrogel; epithelium specific biological ink is prepared by wrapping epithelial cells with matrix hydrogel; then, through a step-by-step assembly strategy, a C-shaped cartilage ring and a fiber ring are alternately printed and a ring-shaped tubular structure is formed by using a double-needle extrusion type biological 3D printing technology, and then a layer of epithelium specific biological ink is attached in the lumen to construct a tubular structure of cartilage-fiber-epithelium multi-tissue composite; it is verified that after being implanted subcutaneously in a naked mouse for 8 weeks, a relatively mature cartilage-vascularized fiber-epithelium tissue composite tissue engineering trachea can be regenerated, and a clinically transformable strategy is provided for the regeneration treatment of complex tracheal injury.