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2296 results about "Solid medium" patented technology

Solid medium has physical structure and allows bacteria to grow in physically informative or useful ways (e.g. as colonies or in streaks). Solid medium is useful for isolating bacteria or for determining the colony characteristics of the isolate.

Fast reproducing method for high quality seedling of dendrobium officinale

The invention provides a method for rapidly reproducing high quality germchit with dendrobium officinale, which is characterized in that: (1) folded sprouts of the dendrobium officinale are taken and sterilized, and stem apexes are inoculated to a solid culture medium to establish a non-bacterial system; (2) the stem sections or small basal tissue blocks of the young seedlings of the non-bacterial system are cut for inducing protocorm based on suitable hormone combination and a culture medium; (3) the protocorm is cultured alternatively on liquid and solid subculture mediums for rapid multiplication to form protocorm masses; (4) the protocorm masses are cut apart and transferred on a solid planting medium for disuniting plants into small seedlings; (5) the small seedlings are transferred on a solid strong seedling culture medium for strengthening the seedlings and taking roots to culture complete plants; (6) the non-bacterial strong seedlings are fixedly planted in suitable seedling adapting substrate to obtain the high quality germchit. The method can be used in large scale industrialization and the explants have the advantages of high soil removal efficiency, high multiplication rate, low variability, strong germchit, good quality, high survival rate after being transplanted, strong growth potential, etc.
Owner:KUNMING UNIV OF SCI & TECH

Method for preparing cow feed additive containing traditional Chinese medicine active probiotics

The invention discloses a method for preparing a cow feed additive containing traditional Chinese medicine active probiotics, comprising the following steps: respectively carrying out amplification culture of the strain number of a single bacterial specie on original strains of active probiotics for cows; mixing primary seed liquors of active probiotics for cows with same weight, inoculating the mixture in a seed tank medium to carry out multiple-strain mixed culture, then inoculating in a traditional Chinese medicine liquid medium to carry out a primary traditional Chinese medicine fermentation; and mixing the multiple-strain mixed traditional Chinese medicine fermentation liquor with isometric cob powder to obtain solid fermentation seeds, and then inoculating in a traditional Chinese medicine solid medium to carry out a secondary traditional Chinese medicine fermentation. According to the invention, the strain activity and colonization performance in the intestinal tracts of activeprobiotics in the cow feed additive are improved greatly, the prepared cow feed additive is rich in traditional Chinese medicine components, the active components with pharmacological effects in the traditional Chinese medicine components fully play the role, thus the performances of strengthening the body, treating diseases and promoting growth of the cow feed additive are improved, and furthermore completely displace antibiotics.
Owner:辽宁后金生物科技有限公司

Microbiological solid-state seed producing and fermenting device

The invention provides a microbiological solid-state seed producing and fermenting device, comprising a fermenting system, an air supply system, a temperature control system, a solid material system, a liquid-state material system and an electric control system, wherein the fermenting system comprises a fermenting tank provided with a stirring device, the top part of the fermenting tank is provided with a material liquid inlet, an exhaust port, a pressure gauge interface and an air inlet sequentially, a feed port is arranged on the upper right side of the front end of the fermenting tank, a discharge port is arranged on the lower left side of the front end of the fermenting tank, and the middle parts of both the feed port and the discharge port are provided with observation windows; the solid material system comprises a feeding device and a conveyer belt; the electric control system mainly comprises a stirring control unit, a temperature control unit, a material liquid control unit, an air supply control unit, a feeding and discharging control unit and a display. The device provided by the invention integrates the functions of steam sterilizing, fermenting, inoculating, drying and crushing of a solid culture medium, the automation degree is high, the microbial contamination rate is low, the production time is greatly shortened, the production efficiency is improved, and the stability of quality of a product is guaranteed.
Owner:CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Method for preparing efficient probiotic preparation from macrofungi bacterial liquid (mycoplasm)

The invention discloses a method for preparing an efficient probiotic preparation from macrofungi bacterial liquid (mycoplasm). The method comprises the following steps: preparing a liquid or a solid culture medium from agricultural and sideline products and culturing fungi to obtain a bacterial liquid or a mycoplasm; carrying out physical and biological treatment on the bacterial liquid or the mycoplasm to enable polysaccharide polypeptide substances to be released from cells and adding a nitrogen source and a carbon source to prepare a probiotics culture medium; and then inoculating the probiotics and culturing to obtain the efficient probiotic preparation. The fungi comprise medicinal (edible) fungi of lentinu edodes, agrocybe cylindracea, morchella, pholiota nameko, hericium erinaceus, bolete, pleurotus nebrodensis, pleurotus citrinopileatus, lucid ganoderma, coriolus versicolor, cordyceps taishanensis, poria cocos, bamboo fungus, lactarius deliciosus and the like; and the probiotics is one or more selected from bacillus, saccharomycete, lactobacillus, enterococcus, bifidobacterium and the like. The probiotic preparation prepared by the method disclosed by the invention is rich in active substances, such as polysaccharides and polypeptides, has better health and treatment effects that those of a conventional probiotic preparation and can be used as therapeutic drugs and health foods or feed additives of human and animal gastrointestinal diseases.
Owner:HUNAN MINKANG BIOTECH RES INST

System for automatically measuring charge distribution on surface of solid medium

The invention belongs to the testing field of discharge characteristics of solid insulating materials, relating to a system for automatically measuring charge distribution on the surface of a solid medium. The system comprises a stainless steel sealed chamber body, a high-voltage electrode, a grounding electrode, an electrostatic meter, a vacuum pump and a computer system thereof, wherein the stainless steel sealed chamber body is provided with a one-dimensional worktable and a two-dimensional worktable; a sample under test is fixed on the two-dimensional worktable; a electrostatic capacitanceprobe is arranged on the one-dimensional worktable; the electrostatic capacitance probe and the one-dimensional worktable are arranged in an extended chamber body of the stainless steel sealed chamber body towards the sample under test on the two-dimensional worktable; one end of the sample under test is connected with the high-voltage electrode, while the other end thereof is connected with thegrounding electrode; the one-dimensional worktable and the two-dimensional worktable are connected to an electronic control displacement table control box by a control line; the electrostatic capacitance probe is connected with the electrostatic meter; and the electronic control displacement table control box and the electrostatic meter are accessed to the computer. In the invention, the system for automatically measuring charge distribution on the surface of the solid medium can measure two-dimensional distribution of charge density on the surface of insulating materials.
Owner:XI AN JIAOTONG UNIV +1

Decomposition maturing agent for fast decomposition-maturing straw

The invention discloses a farm fertilizer produced by biologic technology, in particular a decomposing agent for rapidly decomposing straws at low cost, the mixture ratio of which is aerobic bacillus subtilis Cohn 20%, Bacillus licheniformis 10%, aspergillus flavus 20%, chaetomiaceae 20%, absidia corymbifera 20%, and saccharomycetes 10%. The production procedures are: extracting different beneficial decomposed bacteria from organics from different environments, purifying, rejuvenating, breeding and culturing; inoculating the cultured bacterial strains into disinfected solid culture medium; fermenting for 4-5 days under 25-50 DEG C, airing dry so that the residual moisture in the bacterial strains is 25%; mixing, agitating evenly and packing the aired solid bacterial strains into solid decomposing agent. As the invention is made by using a unique optimization and combination of aerobic bacterial group and a high and new technology and production process, the invention is a pure biologic product, is free from smell and harm for making organic fertilizer by effectively decomposing wastes in the countryside, and the fermenting speed is high, the cost is low, and the source of raw material is wide. The invention has improved the soil structure and reduces environmental pollution.
Owner:李海泉

Method for accurate measuring trace quantity liquid refractivity

A method of precise measurement microscale refractive rate is the method of measurement the refractive of transparent and semi-transparent liquid or solid. The purpose of invention is measuring the refractive rate of transparent and semi-transparent liquid and solid medium. It accords the imaging principle of coaxial spherical optical system and measures the focal spot position of capillary with inhaling the measured liquid and gets the measured liquid's refractive rate by calculation. Select the properly capillary references according to the refractive measurement range of measured medium and then get the need of measurement precision. Example: measuring the multiple liquid medium with refractive n which is higher than 1.33 and lower than 1.48, and the standard deviation of measurement result is between 0.0002 and 0.001; the demand of sample is only 0.004ml. The sample is seal in the capillary and it is benefit to measure the microscale, toxic, irritating, effumable and inhaling liquid's refractive and also it can be used to measure the density of liquid. The invention's equipment is simple and measurement is convenience and reliable and it can be used in the research and producing department of chemical industry, medical, food, and fossil oil industry.
Owner:YUNNAN UNIV

Immobilized microbial inoculum for remediating PAHs contaminated soil and preparation method thereof

The invention relates to remediation technology of polycyclic aromatic hydrocarbon (PAHs) contaminated soil, in particular to an immobilized microbial inoculum for remediating the PAHs contaminated soil and a preparation method thereof. The preparation method specifically comprises the following steps of: inoculating a prepared microbial mixed strain liquid into a solid medium, namely a processed microbial inoculum vector; culturing the microbial mixed strain liquid at a constant temperature of between 28 and 30 DEG C in an incubator for 3 to 4 days; and carrying out adsorption immobilization and proliferation to prepare an immobilized mixed microbial preparation. The method specifically adopts crop byproducts as the vector, and has the advantages of high adsorption speed of mixed strains, good proliferation effect, simple operation, no secondary pollution, rich sources of the crop byproducts and low price. The prepared immobilized cell granules are put into the PAHs contaminated soil in a percentage of 2 to 4 percent, which can effectively remove PAHs contaminants in the soil. The degradation rate of the PAHs processed by 70d can reach 74 percent when the concentration of the PAHs in the contaminated soil is 84mg/kg.
Owner:SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI

Large-scale cultivation method and quality detection method of cordyceps militaris fruit bodies

The invention discloses a large-scale cultivation method and a quality detection method of cordyceps militaris fruit bodies, comprising the steps of: (1) screening spawn; (2) preparing a rice solid culture medium (40-55 parts of rice and 45-60 parts of nutrient solution in every 100 parts by weight) and killing bacteria; (3) preparing liquid spawn: activating the spawn, inoculating the spawn into a liquid culture medium, conducting static culture for 24 hours and performing suspension culture on a shaking table; (4) culturing fruit bodies: inoculating the liquid spawn on the solid culture medium for dark culture for 6 days at the temperature of 20 DEG C until the surface of the medium is covered by mycelium; placing the well grown mycelium under incandescence with light intensity of 100 to 300 lux, wherein during a primordium growth phase, illumination is performed for 20-24 hours per day at the temperature of 20-24 DEG C; and during a fruit bodies growth phase, illumination is performed for 8-12 hours per day at the temperature of 18-20 DEG C and the fruit bodies are harvested 5 to 8 days after mature. The cordyceps militaris fruit bodies cultured by the method have the advantages of high yield and high content of active ingredients. Batches of cordyceps militaris fruit bodies have fingerprints with a similarity value greater than 0.950, indicating stable and controllable quality.
Owner:珠海市先康生物科技有限公司
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