216 results about "Solid medium" patented technology

The invention relates to the field of microbial genetic transformation, and discloses a preparation method of Ilyonectria fungus protoplast and a construction method of a genetic transformation system.The preparation method comprises the following steps that S11, Ilyonectria fungus hyphae are prepared, Ilyonectria fungi are cultured on a PDA solid culture medium for 14 d, then the Ilyonectria fungi are inoculated to a CMC liquid culture medium to be subjected to shake-flask culture for 4 d and filtered, filtrate is centrifuged for 10 min at the speed of 10000 rpm, and spores are collected; diluting the concentration of spores to 107 pieces / mL, inoculating the spores into a YEPD liquid culture medium, carrying out shake-flask culture for 2 days, and filtering and collecting hyphae; and S12, preparing the Ilyonectria fungus protoplast, namely adding the hypha prepared in the step S11 into the enzymatic hydrolysate, performing enzymolysis in a shaker at the temperature of 30 DEG C at the rotating speed of 150-200rpm for 3-5 hours, filtering, centrifuging filtrate at the temperature of 4 DEG C, discarding supernate, adding 0.7 M NaCl for centrifuging, and discarding the supernate, thereby obtaining the Ilyonectria fungus protoplast. The genetic transformation constructed by the invention can be used for exploring the specific action and mechanism of a specific pathogenic gene in the pathogenic process of Ilyonectria fungi through gene knockout or overexpression experiments.

The invention discloses an artificial cultivation method of xylaria fungus sclerotium, and relates to the field of sclerotium cultivation. The method comprises the following steps: collecting xylaria producing sclerotia, obtaining spores by adopting a spore separation method or obtaining core tissues by adopting a tissue separation method, inoculating the spores or the core tissues on an enriched PDA culture medium to be cultured into bacterial colonies, selecting hyphae with vigorous growth vigor at the edges of the bacterial colonies, and performing purification culture until a mother strain only containing a single strain is obtained; performing gene sequencing to identify the strain; propagating the mother strain to obtain an original strain, inoculating the original strain into a sterilized solid culture medium, culturing in a dark place to obtain a cultivated strain, placing the cultivated strain on soil, reserving a space for sclerotium development around the cultivated strain, culturing in the dark place for 33-90 days, and harvesting the sclerotium. The xylaria sclerotium with single germplasm can be cultivated on a large scale, and the problems of wild resource shortage and germplasm mixing are effectively solved.

The invention belongs to the technical field of microorganisms, and relates to a riemerella anatipestifer phage and application thereof. The riemerella anatipestifer bacteriophage provided by the invention is RA-XHX1, is preserved in the China Center for Type Culture Collection (CCTCC), has the preservation number of CCTCC NO: M 20241268, and is classified and named as the riemerella anatipestifer bacteriophage. The bacteriophage is double-stranded DNA (Deoxyribose Nucleic Acid) and myuroviridae, can form transparent plaques on a solid culture medium, has no halo ring around and is clear and regular in edge; the bacteriophage has a relatively good killing effect on riemerella anatipestifer in a space environment, particularly high-pathogenicity riemerella anatipestifer, and can realize a broad-spectrum antibacterial effect when being applied to preparation of medicines for preventing and treating riemerella anatipestifer infection.

The invention discloses a method for simultaneously extracting melanin and a bacterial extracellular vesicle compound from porphyromonas gingivalis and application of the method. The method comprises the following steps: inoculating a solid culture medium with a porphyromonas gingivalis seed solution, and carrying out anaerobic culture overnight at 37 DEG C; picking single colonies, putting the single colonies into a liquid culture medium, performing anaerobic culture at 37 DEG C for 24 hours, and then putting the single colonies into a bacterial incubator to be continuously cultured until OD600 is equal to 1; centrifugally separating supernatant liquid, and centrifuging again to obtain clarified supernatant liquid; and filtering the clarified supernatant liquid, carrying out ultrafiltration centrifugation on the obtained filtrate, washing a filter screen with water to obtain a washing liquid, carrying out ultrasonic treatment to obtain a melanin and bacterial exovesicle compound, carrying out vacuum freeze drying, and storing the melanin and bacterial exovesicle compound in a refrigerator. The method is economical, simple and convenient, the bacterial outer membrane vesicles and the melanin can be extracted at the same time, and the melanin and bacterial outer vesicle compound can achieve the effects of an immune agonist and a photo-thermal agent at the same time under 808 nm near-infrared laser irradiation.

The invention relates to the field of microbial genetic transformation, and discloses a preparation method of Ilyonectria robus fungus protoplast and a construction method of a genetic transformation system.The preparation method comprises the following steps that S11, I.robus hyphae are prepared, after I.robus is cultured on a PDA solid culture medium for 14 days, the I.robus hyphae are inoculated to a CMC liquid culture medium to be subjected to shake-flask culture, and spores are filtered and collected; diluting the spore concentration, inoculating into a YEPD liquid culture medium, carrying out shake-flask culture, filtering and collecting hyphae; and S12, preparing enzymatic hydrolysate, namely adding the hyphae prepared in the step S11 into the enzymatic hydrolysate prepared in the step S12, carrying out shake flask enzymolysis, filtering, centrifuging filtrate, discarding supernate, adding a 0.7 M NaCl solution, centrifuging, and discarding the supernate, so as to obtain the I.robus fungus protoplast. The protoplasts with proper quantity and good quality can be efficiently obtained, and the transformation efficiency of a subsequent genetic transformation system is improved; and the constructed genetic transformation system can be used for construction of a mutant library of the I.robusta strain, interaction of a host plant and the I.robusta strain and research on the pathogenesis of the I.robusta strain.

The invention relates to the technical field of ganoderma lucidum fermentation processes, and particularly discloses an aspergillus cristatus ganoderma lucidum fermentation process which comprises the following steps: S1, transferring a fungus block into a 5% NaCl + MYA solid culture medium in a super clean bench, culturing for 7 days under the condition of constant temperature and constant humidity of 28 DEG C, and repeating the process twice to activate a strain; inoculating the activated strain to a 5% NaCl + MYA plate culture medium, and culturing for 7 days under the condition of constant temperature and constant humidity of 28 DEG C; the method comprises the following steps: adding a proper amount of sterile water into an aspergillus cristatus flat plate cultured for 7 days, scraping spores, adding 1mL of spore liquid into a centrifugal tube filled with glass beads, shaking to break wall of ascospores, counting by using a blood cell counting plate, and diluting into spore suspension with corresponding concentration; s2, weighing a proper amount of ganoderma lucidum blocks, putting the ganoderma lucidum blocks into a fermentation bottle, adding distilled water, sterilizing for 20 minutes at 121 DEG C, cooling to room temperature, inoculating a proper amount of spore suspension, and culturing for 10 days at constant temperature and humidity at 28 DEG C; the aspergillus cristatus fermented ganoderma lucidum sporocarp obtained by the method is high in biological activity, and the bitter taste of ganoderma lucidum is obviously improved.

The invention relates to the technical field of fungus material culture, and particularly discloses a fungus material culture method of armillaria mellea for artificial cultivation of gastrodia elata, which comprises the steps of fungus material selection, culture medium preparation, inoculation, culture shelf arrangement, environment arrangement, harvesting and gastrodia elata planting. According to the method, hard and corrosion-resistant broad-leaved tree species are selected as the fungus material, a composite culture medium formula is adopted, namely, the advantages of a liquid culture medium and a solid culture medium are combined, comprehensive and balanced nutrition support is provided for growth of armillaria mellea, the culture efficiency and quality of the fungus material are improved, meanwhile, the proportion of the formula of the culture medium is designed, and the culture cost is reduced. The water content and the pH value of the culture medium are controlled, favorable growth environment conditions are created for growth of armillaria mellea, meanwhile, a syringe or an inoculating loop for high-pressure steam sterilization effectively prevents contamination of infectious microbes, and the success rate of inoculation and the purity of hyphae are improved.

The invention provides a microalgae culture medium and a microalgae preservation method, relates to the technical field of algae culture, and particularly provides a brand-new microalgae preservation liquid culture medium and a microalgae preservation solid culture medium, and develops a brand-new microalgae biphasic preservation method by using the microalgae preservation liquid culture medium and the microalgae preservation solid culture medium. The preservation method comprises the following steps: inoculating microalgae species in an exponential growth phase into a microalgae preservation solid culture medium, culturing for 5-10 days, and adding a microalgae preservation liquid culture medium into the microalgae preservation solid culture medium inoculated with the microalgae species to form a semi-solid culture medium, the semi-solid culture medium is subjected to biphasic preservation under natural light at 2-10 DEG C. The preservation method can effectively prolong the preservation time of microalgae species, ensures the preservation effect, and has wide application prospects.

The invention discloses a plant lactobacillus and application thereof, and belongs to the technical field of microorganisms. The plant lactobacillus 002P provided by the invention is a new strain, and is round, white, neat in edge, smooth in surface and soft in texture on an MRS solid culture medium; growth of bacteria can be remarkably inhibited, an inhibition effect on staphylococcus aureus, pseudomonas aeruginosa and escherichia coli is achieved, and the inhibition zone is 23.33-279.33 mm; the healing of infected wounds can be obviously promoted; the selenium conversion rate is excellent, and the maximum selenium conversion rate can reach 88%; the nano-selenium prepared from the plant lactobacillus 002P is spherical, has the particle size of 200-500nm, has excellent properties of resisting oxidation, relieving hepatotoxicity induced by saturated fatty acid, promoting expression of selenoprotein in a mouse body and reducing blood fat, and has a wide application prospect.

The invention relates to a heavy metal-antibiotic multiple resistant bacteria and its application, which belongs to the field of microbial remediation technology. The heavy metal-antibiotic multiple resistant bacteria described in this paper is Rossellomorea oryzaecorticis ZC255, which is preserved at China General Microbiological Culture Collection Center on Jun. 12, 2023, the biological preservation number is CGMCC No. 27617. This strain not only has high activity, simple culture method and strong adaptability, but also can survive in solid medium with high concentration of heavy metals and antibiotics. It also has excellent metal tolerance; it can effectively degrade a variety of antibiotics and can be applied to the remediation of heavy metal-contaminated or antibiotic-contaminated or heavy metal and antibiotic-contaminated water or soil. In addition, it has the advantages of large adsorption capacity, low cost, high efficiency, good effect and no secondary pollution.

The invention discloses a method for microfluidic high-throughput screening of AKK bacteria. The method comprises the following steps: 1) preparing a microfluidic chip; 2) collecting fresh excrement of healthy volunteers, diluting, and adding a fluorescence labeled antibody to obtain a dispersion phase; and (3) preparing liquid drops by taking paraffin oil and Span-80 as a mobile phase, enabling the liquid drops to enter a liquid drop culture tank through an S-shaped liquid drop circulation channel, culturing, introducing the mobile phase, introducing the liquid drops in the liquid drop culture tank into a liquid drop collection tank, taking out, detecting a fluorescence signal, culturing the liquid drops with the fluorescence signal on the improved brain heart infusion broth solid culture medium, and obtaining the brain heart infusion broth. Obtaining a to-be-identified strain; 4, the strain to be identified serves as a template, PCR amplification is conducted, sequencing is conducted on an amplification product, BLAST sequence comparison is conducted on a sequencing result on an NCBI website, and the target strain AKK bacterium is screened out. The method has the advantages of being high in throughput, simple in experimental process and high in specificity, and the screening efficiency of the AKK bacterium can be improved.

The invention relates to the technical field of bioengineering, in particular to a method for promoting cordyceps sinensis to produce conidia. The invention provides a method for promoting cordyceps sinensis to produce conidia, which comprises the following steps: (1) carrying out dark culture on cordyceps sinensis on a primary solid culture medium to obtain mycelium; and (2) transferring the mycelium obtained in the step (1) to a secondary solid culture medium, and carrying out environmental stimulation culture until conidia are generated. The method is suitable for industrial production, has the advantages of simplicity and convenience in operation, controllable cost, high repeatability and the like, can effectively shorten the fermentation period, improves the inoculation efficiency and the yield of metabolites, and provides reliable technical support for large-scale artificial culture and deep development of cordyceps sinensis.

The present invention discloses a method and apparatus for screening strains with high substrate utilization efficiency using CO2 release rate, belonging to the field of microbial screening. The method comprises: standardized strain culture: inoculating the strain to be screened onto a solid culture medium, controlling environmental parameters and culturing in the dark; dynamic CO2 monitoring: collecting CO2 concentration using a CO2 sensor with integrated temperature, humidity, and pressure compensation; metabolic signature extraction: calculating the CO2 release rate per unit time, the release rate slope, and the normalized cumulative release of hyphae; hyphae growth monitoring and data synchronization: calculating the colony specific growth rate and generating a synchronized data set with CER data; hyphae growth metabolic correlation analysis: establishing a coupled kinetic model of hyphae growth and CO2 release; efficiency prediction and strain screening: using a multivariate regression model to predict substrate utilization efficiency and screening efficient strains according to grading criteria. The present invention has the advantages of being non-destructive and highly real-time, significantly shortening the screening cycle for strains with high substrate utilization efficiency.

To provide a high-hardness formulation used as a material containing microorganisms, and a method for producing the same. [Solution] The formulation according to the present disclosure comprises a solid culture containing a microorganism that forms mycelial masses and a solid medium on which the microorganisms are cultured. The formulation has a water content of 1 to 15% by weight. The formulation also has a hardness of 30 to 200 N. The manufacturing method of the formulation according to the present disclosure comprises step (1): drying the culture containing a microorganism that forms mycelial masses and a solid medium on which the microorganisms are cultured so that the water content is 1 to 15% by weight. The manufacturing method also comprises step (2): solidifying the dried culture.

The invention discloses mine water pseudomonas as well as a screening method and application thereof in recharge capacity increase, and belongs to the technical field of mine water recharge engineering. A mine water sample is taken back and then filtered through a filter membrane and a vacuum suction filter; after constant-temperature oscillation, inoculating a liquid LB meat extract peptone culture medium for enrichment culture; carrying out strain separation on the bacterium liquid subjected to enrichment culture by adopting a dilution coating plate method; the method comprises the following steps: carrying out streak culture on a single colony on a solid LB culture medium to obtain a pseudomonas colony, the pseudomonas is Pseudomonas sp. B2, the taxonomic name is Pseudomonas sp. B2, the preservation number is GDMCC No.66726, the preservation date is July 22, 2025, and the preservation unit is Guangdong Province Microbial Culture Collection Center, and the screened pseudomonas capable of producing carbonic anhydrase can effectively promote the dissolution of calcite and dolomite, and can be used for producing carbonic anhydrase. The recharge amount is increased, and the recharge efficiency is improved.

This invention discloses a culture medium for manganese-oxidizing bacteria suitable for low-carbon source environments in water supply networks and its application. The culture medium is prepared by mixing ferric ammonium citrate, manganese sulfate, ammonium sulfate, sodium chloride, magnesium sulfate, dipotassium hydrogen phosphate, and sodium nitrate, followed by high-temperature and high-pressure sterilization to obtain a culture solution. Manganese sulfate is added to the culture solution to obtain the manganese-oxidizing bacteria culture medium. Manganese-oxidizing bacteria are screened using this culture medium. Cast iron pipe scale is subcultured in the medium to enrich the manganese-oxidizing bacteria culture solution. The enriched manganese-oxidizing bacteria culture solution is diluted and spread onto agar solid medium, and incubated at a constant temperature to obtain manganese-oxidizing bacteria colonies. This invention solves the problem of altered bacterial physiological metabolism caused by high concentrations of added manganese ions, effectively improving bacterial activity and manganese oxidation capacity.

The invention relates to the technical field of microbiological research, and particularly discloses a method for reducing greenhouse gas emission in a compost maturing process by using nematodes, which comprises the following steps: A, inoculating escherichia coli into an LB liquid culture medium for culturing, uniformly coating an NGM nematode growth solid culture medium with the LB liquid culture medium, picking the nematodes into the NGM nematode growth solid culture medium, and culturing for 2-3 hours; finally, culturing the NGM nematode growth solid culture medium inoculated with the escherichia coli and the nematode in a culture box in darkness; b, counting the nematodes, adding nematode cleaning liquid into a super clean bench, cleaning the nematodes, and filling the nematodes into a centrifugal tube; c, repeating the step B to obtain nematode liquid; d, disinfecting the nematodes with alcohol; e, uniformly adding the nematode liquid into the compost; and F, determining the gas emission condition in the compost maturing process by using the static box. By culturing and propagating the nematodes purchased in the market, the method can be popularized and applied to compost, and the emission content of greenhouse gases in the decomposition process of the nematodes is reduced.

The invention discloses a method for culturing fungal-feeding nematodes by using two fungi in a mixed manner and reducing pollution of a culture medium. The method comprises the following steps: A, separating and extracting euphorbia fungal-feeding nematodes; b, identifying the euphorbia fungal nematodes; c, preparing a strain mixture which comprises botrytis cinerea and trichoderma harzianum in a mass ratio of 1: 1; d, inoculating the strain mixture into a PDA liquid culture medium to prepare a nematode PDA culture medium; e, centrifuging the nematodes obtained in the step B; f, disinfecting the nematodes, namely disinfecting the surfaces of the nematodes by using a mixed solution of streptomycin sulfate and actinomycete ketone; g, adding sterile water into the nematodes disinfected in the step F, and centrifuging; h, repeating the step G for multiple times to obtain nematode liquid; i, inoculating the disinfected nematodes into a flat plate of a PDA solid culture medium; and J, observing the growth quantity of the nematodes in the culture medium by using an inverted microscope. According to the method, the nematodes are cultured by culturing the fungus-eating nematode strain mixture, and the method is used for culturing and propagating the nematodes.

The present invention belongs to the field of microbial transformation technology, specifically a method for preparing a steroid drug intermediate, comprising the following steps: strain activation; seed liquid culture; fermentation conversion in a fermenter; extraction of conversion products; purification of conversion products; structural confirmation of conversion products. By using androstenedione as the starting material, a strain is selected to ferment and prepare 1,4-androstenedione, and the culture medium selected by strain activation is LB solid culture medium, which is cultured in a constant temperature incubator at 28°C for 24 hours after inoculation; LB liquid culture medium is selected for seed liquid culture, and a single colony on a plate is picked up with an inoculation loop and inoculated and then cultured in a constant temperature shaker at 28°C for 16 hours; then the substrate 4-androstenedione is fermented; an equal volume of ethyl acetate is added to the culture medium for extraction and separation, and it is in a white powder after decolorization and purification, and the selected strain has a short conversion time, and the conversion rate can reach 70% in only 24 hours, realizing the function of rapid conversion and achieving the effect of improving the conversion rate.

The invention discloses a core-shell structure algae / bacteria symbiotic hydrogel and a preparation method thereof, the core-shell structure algae / bacteria symbiotic hydrogel (algae is a core and bacteria are a shell) is prepared by using a 3D printing technology, the algae and the bacteria are separated from each other in space, and a customized latticed structure can effectively disperse light, so that microalgae in a bracket can receive the light, and the algae / bacteria symbiotic hydrogel is prepared. The problem of low hydrogen production efficiency caused by serious mutual shielding among cells, low light energy utilization rate, nutrient substance competition and the like is solved, the system almost eliminates the requirement on a liquid medium, and growth of microalgae and bacteria in the hydrogel can be supported only by forming a layer of solid culture medium at the bottom of the printed hydrogel; water resources are saved; and the space utilization rate is improved.

The invention provides a method for screening acid-producing strains, which comprises the following steps: 1) mutagenizing original strains, and constructing a mutant library; 2) primarily screening mutants in the mutant library by using a calcium carbonate transparent ring method or a pH indicator method to obtain primarily screened strains; (3) inoculating the primarily screened strains into a solid culture medium, culturing, selecting single colonies with the same diameter after the colonies grow out, punching the single colonies with the same diameter by using a punching device to obtain gel blocks of the solid culture medium with the same size or basically the same size, and culturing all the gel blocks under the same or basically the same condition to obtain gel blocks of the solid culture medium; recording the time when the gel block completely becomes transparent or changes color, and screening out secondary screening strains according to the time; 4, the secondary screening strains are subjected to fermentation culture, the yield of the organic acid is detected, and the acid-producing strains are screened out according to the yield of the organic acid.The method is simple and easy to operate, and the target strains can be more accurately and rapidly screened out.

The invention relates to the field of tomato bacterial wilt prevention and control, in particular to a research experiment method for the tomato bacterial wilt prevention and control performance of a complex microbial inoculant, which comprises the following steps: activation of bacterial strains, preparation of the complex microbial inoculant, inhibition of the complex microbial inoculant on GMI1000, colonization of the complex microbial inoculant at tomato roots, response of the complex microbial inoculant on tomato root exudates, and pot experiment. Measuring plant biomass and physicochemical properties; the method comprises the following steps: S1, measuring the content of plant rhizosphere soil nutrients, measuring the number of complex microbial inoculants and GMI1000 in soil, and performing high-throughput sequencing on tomato rhizosphere soil microorganisms, in the step S1, GMI1000 divides strains preserved in a glycerol tube at-80 DEG C into a sterilized BG solid culture medium through a plate streaking method, and the strains are inversely placed in a constant-temperature incubator at 28 DEG C to be cultured for 48 hours. Then, single colonies are selected and placed in a BG liquid culture medium; and regulating and controlling the community structure of rhizosphere fungi to repair or remodel tomato rhizosphere soil microbial communities infected by ralstonia solanacearum so as to indirectly prevent and treat tomato bacterial wilt.

This invention discloses a strain of *Bacillus parabrachium* ABX1 and its applications, belonging to the field of beneficial microbial technology. The *Bacillus parabrachium* ABX1 provided by this invention is a Gram-positive bacterium isolated from the intestinal fluid of *Bombyx mori*. On CMC-Na solid medium, the ratio of the diameter of the hydrolysis zone to the colony diameter can reach 11. Enzyme activity identification results show that the filter paper enzyme activity of *Bacillus parabrachium* ABX1 is as high as 109.79 U·mL. ‑1 The exo-β-glucanase activity was 107.59 U·mL. ‑1 The cellulase activity reached 107.37 U·mL ‑1 It exhibits excellent cellulose and / or crystalline cellulose degradation capabilities; it is easy to use and has a wide range of enzyme production conditions, showing excellent enzyme activity at pH 5–10 and 25–50℃; the enzyme production time is short, with high enzyme yield achieved in just 12 hours, and there is no significant adverse effect on enzyme yield with prolonged reaction time; it is highly safe to use and has great application prospects in areas such as oak garden waste branch treatment, improvement of oak silkworm digestibility, industrial degradation of cellulose, and improvement of soil fertility.

The invention provides a mycobacterium tuberculosis attenuated live vaccine strain solid culture medium as well as preparation and application thereof, and the raw material of the solid culture medium comprises supernate obtained by filtering potato water prepared from selenium-enriched yellow-core potatoes with the total selenium content of 15-150mu g / kg. A solid culture medium without animal-derived components is obtained; and the culture method is further optimized, so that the mycobacterium tuberculosis is high in thallus proliferation speed and not easy to be infected by infectious microbes when being cultured. The solid culture medium disclosed by the invention is simple in manufacturing process, low in implementation cost and high in conversion efficiency, and has a prominent popularization prospect.

A Neorhizobium huautlense strain CNBG-PGPR-21 and uses thereof. The Neorhizobium sp. CNBG-PGPR-21 was deposited in China General Microbiological Culture Collection Center (CGMCC) on August 1st, 2022,

The utility model discloses an improved culture bottle special for mycoplasma culture, and relates to the technical field of bioengineering. The bottle comprises a bottle body and a bottle cap, the inner side and the outer side of the periphery of a bottle opening in the upper side of the bottle body are respectively provided with a threaded structure, and the bottle body is transparent or semitransparent; the bottle cap is mounted on the bottle body through the bottle body outer side threads; a gas-permeable membrane assembly is arranged on the bottle cap, and the gas-permeable membrane assembly is composed of at least one layer of microporous filtering membrane and a supporting frame; an anti-adsorption coating is arranged on the inner wall surface of the bottle body; a solid culture medium is arranged in the bottom of the bottle body, the upper surface of the solid culture medium inclines to the bottle bottom, and a liquid culture medium is filled between the solid culture medium slope and the side wall of the bottle body; the anti-splashing device is installed in the bottle body through threads on the inner side of the bottle opening and is in an inverted circular truncated cone shape, and the interior of the anti-splashing device is hollow. By improving the material of the bottle body, optimizing the inner wall coating and arranging the breathable membrane assembly, the safety, reliability and visualization degree of the mycoplasma culture process are improved, and meanwhile, the risk of cross contamination is effectively reduced.

The invention discloses a morchella hypha powder prefabrication method and equipment line, belongs to the technical field of morchella hyphae, and solves the problems that morchella is extremely sensitive to continuous cropping, metabolite changes the soil microenvironment, hypha division and nutrient absorption are directly hindered, the hypha growth speed is reduced by 30%-50%, the biomass is reduced, and primordium formation is delayed. Comprising the following steps: S1, carrying out stress-resistant intensified culture on liquid strains; chitosan in a specific proportion in a liquid strain neutralizes dopamine quinone, antioxidant enzyme is induced to be improved, fat-soluble toxin is embedded in beta-cyclodextrin in a solid culture medium, dendrobium nobile residues adsorb water-soluble toxin, sodium selenite enhances oxidation resistance, germanium dioxide promotes hypha branching, an overburden filtrate degrades dopamine quinone, ash hydrolyzes related toxin, and the yield is increased. Pseudomonas compensates for beneficial metabolites and accelerates metabolism through temperature difference stimulation, so that hypha growth is accelerated, differential metabolites are reduced, biomass is increased, and the three-crop fruiting rate is stable.

The invention relates to a method for detecting microbial colonies in a biological sample placed in a solid medium by means of a detection system comprising at least one incubator, an analysis unit and at least one automatic image capture system, comprising a first stage, the present invention relates to a method for detecting and counting objects exhibiting growth in the field of view of an image capture system, and to a second stage applied only to those samples deemed negative in the first stage of the method, the second stage being based on the detection of anomalies on at least the last image acquired in a culture sequence.

The invention provides a bacillus high-yield strain for producing riboflavin and application of the bacillus high-yield strain, and belongs to the technical field of microbial fermentation. The bacillus strain is named as VB2-M-230602-66, the preservation unit is China Center for Type Culture Collection, the preservation date is December 1, 2023, and the preservation number is CCTCC NO: M20232421; according to the invention, the strain VB2-M-230602-66 with stable character is obtained by adopting physical mutagenesis separation and combining with shake flask screening of tolerance dose of a resistance marker, riboflavin can be produced through liquid fermentation, and the yield is stable; the bacterial strain has the biological characteristics that the bacterial strain is gram-positive bacteria through gram staining, the bacteria are cultured at 37 DEG C for 18 hours, the thalli are rod-shaped, 0.5-0.8 mu m * 2.0-5.4 mu m and are arranged in a single or paired manner, and bacterial colonies cultured on a solid culture medium are pale yellow, nearly circular, wet in surface, opaque and irregular in edge; the bacillus strain VB2-M-230602-66 provided by the invention is suitable for fermentation production of riboflavin (vitamin B2), has stable production performance, can obtain greater economic benefits, and is worthy of popularization.

The present invention belongs to the field of development and application of Chinese medicine resources, and specifically relates to a preparation method and application of a Morchella solid-state fermented geranium seedling extract with antibacterial effect. The preparation method is realized by the following steps: activating the Morchella strain by PDA solid culture medium, then inoculating it into the PDA culture medium containing geranium seedling for fermentation, extracting by 70% ethanol, centrifuging the extract, and freeze-drying after rotary evaporation to obtain a Morchella fermented geranium seedling extract (solid-state fermentation). The Morchella fermented geranium seedling extract prepared by the present invention is subjected to a broad-spectrum antibacterial experiment on pathogenic bacteria such as Staphylococcus aureus, Escherichia coli, and Bacillus aerogenes. It is found that the Morchella fermented geranium seedling extract of the present invention can inhibit the growth of Staphylococcus aureus, Escherichia coli, and Bacillus aerogenes, and has a significant antibacterial effect, which provides a scientific basis for the research on the antibacterial application of Morchella fermented geranium seedling extract.