76 results about "Solid medium" patented technology

The invention discloses an artificial cultivation method of xylaria fungus sclerotium, and relates to the field of sclerotium cultivation. The method comprises the following steps: collecting xylaria producing sclerotia, obtaining spores by adopting a spore separation method or obtaining core tissues by adopting a tissue separation method, inoculating the spores or the core tissues on an enriched PDA culture medium to be cultured into bacterial colonies, selecting hyphae with vigorous growth vigor at the edges of the bacterial colonies, and performing purification culture until a mother strain only containing a single strain is obtained; performing gene sequencing to identify the strain; propagating the mother strain to obtain an original strain, inoculating the original strain into a sterilized solid culture medium, culturing in a dark place to obtain a cultivated strain, placing the cultivated strain on soil, reserving a space for sclerotium development around the cultivated strain, culturing in the dark place for 33-90 days, and harvesting the sclerotium. The xylaria sclerotium with single germplasm can be cultivated on a large scale, and the problems of wild resource shortage and germplasm mixing are effectively solved.

The invention discloses a method for microfluidic high-throughput screening of AKK bacteria. The method comprises the following steps: 1) preparing a microfluidic chip; 2) collecting fresh excrement of healthy volunteers, diluting, and adding a fluorescence labeled antibody to obtain a dispersion phase; and (3) preparing liquid drops by taking paraffin oil and Span-80 as a mobile phase, enabling the liquid drops to enter a liquid drop culture tank through an S-shaped liquid drop circulation channel, culturing, introducing the mobile phase, introducing the liquid drops in the liquid drop culture tank into a liquid drop collection tank, taking out, detecting a fluorescence signal, culturing the liquid drops with the fluorescence signal on the improved brain heart infusion broth solid culture medium, and obtaining the brain heart infusion broth. Obtaining a to-be-identified strain; 4, the strain to be identified serves as a template, PCR amplification is conducted, sequencing is conducted on an amplification product, BLAST sequence comparison is conducted on a sequencing result on an NCBI website, and the target strain AKK bacterium is screened out. The method has the advantages of being high in throughput, simple in experimental process and high in specificity, and the screening efficiency of the AKK bacterium can be improved.

The invention relates to the technical field of bioengineering, in particular to a method for promoting cordyceps sinensis to produce conidia. The invention provides a method for promoting cordyceps sinensis to produce conidia, which comprises the following steps: (1) carrying out dark culture on cordyceps sinensis on a primary solid culture medium to obtain mycelium; and (2) transferring the mycelium obtained in the step (1) to a secondary solid culture medium, and carrying out environmental stimulation culture until conidia are generated. The method is suitable for industrial production, has the advantages of simplicity and convenience in operation, controllable cost, high repeatability and the like, can effectively shorten the fermentation period, improves the inoculation efficiency and the yield of metabolites, and provides reliable technical support for large-scale artificial culture and deep development of cordyceps sinensis.

To provide a high-hardness formulation used as a material containing microorganisms, and a method for producing the same. [Solution] The formulation according to the present disclosure comprises a solid culture containing a microorganism that forms mycelial masses and a solid medium on which the microorganisms are cultured. The formulation has a water content of 1 to 15% by weight. The formulation also has a hardness of 30 to 200 N. The manufacturing method of the formulation according to the present disclosure comprises step (1): drying the culture containing a microorganism that forms mycelial masses and a solid medium on which the microorganisms are cultured so that the water content is 1 to 15% by weight. The manufacturing method also comprises step (2): solidifying the dried culture.

The invention discloses mine water pseudomonas as well as a screening method and application thereof in recharge capacity increase, and belongs to the technical field of mine water recharge engineering. A mine water sample is taken back and then filtered through a filter membrane and a vacuum suction filter; after constant-temperature oscillation, inoculating a liquid LB meat extract peptone culture medium for enrichment culture; carrying out strain separation on the bacterium liquid subjected to enrichment culture by adopting a dilution coating plate method; the method comprises the following steps: carrying out streak culture on a single colony on a solid LB culture medium to obtain a pseudomonas colony, the pseudomonas is Pseudomonas sp. B2, the taxonomic name is Pseudomonas sp. B2, the preservation number is GDMCC No.66726, the preservation date is July 22, 2025, and the preservation unit is Guangdong Province Microbial Culture Collection Center, and the screened pseudomonas capable of producing carbonic anhydrase can effectively promote the dissolution of calcite and dolomite, and can be used for producing carbonic anhydrase. The recharge amount is increased, and the recharge efficiency is improved.

This invention discloses a culture medium for manganese-oxidizing bacteria suitable for low-carbon source environments in water supply networks and its application. The culture medium is prepared by mixing ferric ammonium citrate, manganese sulfate, ammonium sulfate, sodium chloride, magnesium sulfate, dipotassium hydrogen phosphate, and sodium nitrate, followed by high-temperature and high-pressure sterilization to obtain a culture solution. Manganese sulfate is added to the culture solution to obtain the manganese-oxidizing bacteria culture medium. Manganese-oxidizing bacteria are screened using this culture medium. Cast iron pipe scale is subcultured in the medium to enrich the manganese-oxidizing bacteria culture solution. The enriched manganese-oxidizing bacteria culture solution is diluted and spread onto agar solid medium, and incubated at a constant temperature to obtain manganese-oxidizing bacteria colonies. This invention solves the problem of altered bacterial physiological metabolism caused by high concentrations of added manganese ions, effectively improving bacterial activity and manganese oxidation capacity.

The invention provides a method for screening acid-producing strains, which comprises the following steps: 1) mutagenizing original strains, and constructing a mutant library; 2) primarily screening mutants in the mutant library by using a calcium carbonate transparent ring method or a pH indicator method to obtain primarily screened strains; (3) inoculating the primarily screened strains into a solid culture medium, culturing, selecting single colonies with the same diameter after the colonies grow out, punching the single colonies with the same diameter by using a punching device to obtain gel blocks of the solid culture medium with the same size or basically the same size, and culturing all the gel blocks under the same or basically the same condition to obtain gel blocks of the solid culture medium; recording the time when the gel block completely becomes transparent or changes color, and screening out secondary screening strains according to the time; 4, the secondary screening strains are subjected to fermentation culture, the yield of the organic acid is detected, and the acid-producing strains are screened out according to the yield of the organic acid.The method is simple and easy to operate, and the target strains can be more accurately and rapidly screened out.

This invention discloses a strain of *Bacillus parabrachium* ABX1 and its applications, belonging to the field of beneficial microbial technology. The *Bacillus parabrachium* ABX1 provided by this invention is a Gram-positive bacterium isolated from the intestinal fluid of *Bombyx mori*. On CMC-Na solid medium, the ratio of the diameter of the hydrolysis zone to the colony diameter can reach 11. Enzyme activity identification results show that the filter paper enzyme activity of *Bacillus parabrachium* ABX1 is as high as 109.79 U·mL. ‑1 The exo-β-glucanase activity was 107.59 U·mL. ‑1 The cellulase activity reached 107.37 U·mL ‑1 It exhibits excellent cellulose and / or crystalline cellulose degradation capabilities; it is easy to use and has a wide range of enzyme production conditions, showing excellent enzyme activity at pH 5–10 and 25–50℃; the enzyme production time is short, with high enzyme yield achieved in just 12 hours, and there is no significant adverse effect on enzyme yield with prolonged reaction time; it is highly safe to use and has great application prospects in areas such as oak garden waste branch treatment, improvement of oak silkworm digestibility, industrial degradation of cellulose, and improvement of soil fertility.

The invention provides a mycobacterium tuberculosis attenuated live vaccine strain solid culture medium as well as preparation and application thereof, and the raw material of the solid culture medium comprises supernate obtained by filtering potato water prepared from selenium-enriched yellow-core potatoes with the total selenium content of 15-150mu g / kg. A solid culture medium without animal-derived components is obtained; and the culture method is further optimized, so that the mycobacterium tuberculosis is high in thallus proliferation speed and not easy to be infected by infectious microbes when being cultured. The solid culture medium disclosed by the invention is simple in manufacturing process, low in implementation cost and high in conversion efficiency, and has a prominent popularization prospect.

The utility model discloses an improved culture bottle special for mycoplasma culture, and relates to the technical field of bioengineering. The bottle comprises a bottle body and a bottle cap, the inner side and the outer side of the periphery of a bottle opening in the upper side of the bottle body are respectively provided with a threaded structure, and the bottle body is transparent or semitransparent; the bottle cap is mounted on the bottle body through the bottle body outer side threads; a gas-permeable membrane assembly is arranged on the bottle cap, and the gas-permeable membrane assembly is composed of at least one layer of microporous filtering membrane and a supporting frame; an anti-adsorption coating is arranged on the inner wall surface of the bottle body; a solid culture medium is arranged in the bottom of the bottle body, the upper surface of the solid culture medium inclines to the bottle bottom, and a liquid culture medium is filled between the solid culture medium slope and the side wall of the bottle body; the anti-splashing device is installed in the bottle body through threads on the inner side of the bottle opening and is in an inverted circular truncated cone shape, and the interior of the anti-splashing device is hollow. By improving the material of the bottle body, optimizing the inner wall coating and arranging the breathable membrane assembly, the safety, reliability and visualization degree of the mycoplasma culture process are improved, and meanwhile, the risk of cross contamination is effectively reduced.

The invention discloses a morchella hypha powder prefabrication method and equipment line, belongs to the technical field of morchella hyphae, and solves the problems that morchella is extremely sensitive to continuous cropping, metabolite changes the soil microenvironment, hypha division and nutrient absorption are directly hindered, the hypha growth speed is reduced by 30%-50%, the biomass is reduced, and primordium formation is delayed. Comprising the following steps: S1, carrying out stress-resistant intensified culture on liquid strains; chitosan in a specific proportion in a liquid strain neutralizes dopamine quinone, antioxidant enzyme is induced to be improved, fat-soluble toxin is embedded in beta-cyclodextrin in a solid culture medium, dendrobium nobile residues adsorb water-soluble toxin, sodium selenite enhances oxidation resistance, germanium dioxide promotes hypha branching, an overburden filtrate degrades dopamine quinone, ash hydrolyzes related toxin, and the yield is increased. Pseudomonas compensates for beneficial metabolites and accelerates metabolism through temperature difference stimulation, so that hypha growth is accelerated, differential metabolites are reduced, biomass is increased, and the three-crop fruiting rate is stable.

The invention relates to a method for detecting microbial colonies in a biological sample placed in a solid medium by means of a detection system comprising at least one incubator, an analysis unit and at least one automatic image capture system, comprising a first stage, the present invention relates to a method for detecting and counting objects exhibiting growth in the field of view of an image capture system, and to a second stage applied only to those samples deemed negative in the first stage of the method, the second stage being based on the detection of anomalies on at least the last image acquired in a culture sequence.

The invention provides a bacillus high-yield strain for producing riboflavin and application of the bacillus high-yield strain, and belongs to the technical field of microbial fermentation. The bacillus strain is named as VB2-M-230602-66, the preservation unit is China Center for Type Culture Collection, the preservation date is December 1, 2023, and the preservation number is CCTCC NO: M20232421; according to the invention, the strain VB2-M-230602-66 with stable character is obtained by adopting physical mutagenesis separation and combining with shake flask screening of tolerance dose of a resistance marker, riboflavin can be produced through liquid fermentation, and the yield is stable; the bacterial strain has the biological characteristics that the bacterial strain is gram-positive bacteria through gram staining, the bacteria are cultured at 37 DEG C for 18 hours, the thalli are rod-shaped, 0.5-0.8 mu m * 2.0-5.4 mu m and are arranged in a single or paired manner, and bacterial colonies cultured on a solid culture medium are pale yellow, nearly circular, wet in surface, opaque and irregular in edge; the bacillus strain VB2-M-230602-66 provided by the invention is suitable for fermentation production of riboflavin (vitamin B2), has stable production performance, can obtain greater economic benefits, and is worthy of popularization.

The present application relates to a kind of culture medium preparation method for studying the influence of algal liquid secretion on arabidopsis growth, belong to the field of biotechnology.The method includes the preparation of solid medium, extraction of algal liquid secretion, preheating, four major steps of medium mixing.The liquid blue algae extracellular secretion is mixed into solid medium in specific method, the interference of microcystis aeruginosa liquid medium on experimental results is excluded by designing negative control, the "dose-effect" study of secretion to plant is carried out by adjusting the ratio of extracellular secretion and medium, the chemical stability of active component in extracellular secretion is inferred by designing stability experiment.The culture method designed in the application can be widely applied in other similar researches exploring the influence of microbial secretion on arabidopsis growth.

The present invention provides a formulation based on bacteria of the Bacillus genus that are capable of generating active sludges that degrade molecules present in water / soil and which can adapt to the environment to which they are exposed. The bacteria are inoculated in an organic substrate for the growth thereof, through an aerobic fermentation process in solid medium. The formulation can reduce the lipid, fat and protein content in wastewater, as well as eliminate bad odours and coliforms from same. It is therefore proposed for use as a bioremediation agent for wastewater from nejayote, blackwater and fat traps.

The application discloses a bacteriostatic type of liquor dreg containing Zhangzhou Bacillus GBW-NX and a preparation method and application thereof, and belongs to the technical field of microorganisms. The preservation number of the Zhangzhou Bacillus GBW-NX is CGMCC No. 27402. It is proved through experiments that the Zhangzhou Bacillus GBW-NX has the bacteriostatic effect on inhibiting Escherichia coli, Salmonella and Staphylococcus aureus. The Zhangzhou Bacillus GBW-NX is placed in a solid culture medium containing the liquor dreg to be fermented and enzymolyzed to prepare the bacteriostatic type of liquor dreg. The bacteriostatic substance of the bacteriostatic type of liquor dreg is a bacteriocin. It is also proved that the bacteriostatic type of liquor dreg can improve the intestinal function of ruminants including lactating cows, and can also improve the milk yield of the cows, thereby widening the application range of the Zhangzhou Bacillus GBW-NX, providing a new direction for the feeding of ruminants, and having important significance for the breeding of ruminants.

The invention provides a PEG / CaCl2 mediated Penicillium citrinum genetic transformation method, and belongs to the technical field of microbial genetic transformation, and the method comprises the following steps: adding a target gene segment into Penicillium citrinum protoplast suspension, and carrying out an ice bath reaction; the target gene segment is obtained by taking pJET-hph-02944 plasmid as a template and carrying out PCR (Polymerase Chain Reaction) amplification by adopting a hygromycin B resistance gene primer pair with gene sequences as shown in SEQ ID No. 1 and SEQ ID No. 2; adding a PTC (Positive Temperature Coefficient) buffer solution into the mixed system, uniformly mixing and standing; continuously adding a TB3 liquid culture medium containing ampicillin, and carrying out recovery culture; uniformly mixing the resuscitated protoplast with a molten TB3 solid culture medium, pouring a plate, and carrying out dark culture for the first time; then covering a TB3 solid culture medium containing hygromycin B and ampicillin, carrying out secondary dark culture to obtain a transformant, and transferring the transformant to a PDA plate containing hygromycin B for screening. According to the method, CaCl2-PEG is adopted for mediated transformation of Penicillium citrinum, the transformation efficiency is higher, and the transformation speed is higher.

The invention relates to the technical field of food fermentation, in particular to a multi-stage strain fermentation and sterilization packaging method of red yeast rice. The method comprises the following steps that a high-quality monascus strain is selected and inoculated to a solid culture medium, the solid culture medium is prepared from 200 g of potatoes, 20 g of glucose, 15-20 g of agar and 1000 mL of tap water, and the pH value of the solid culture medium is 5.0-6.0; culturing in a constant-temperature incubator at 28-32 DEG C for 5-7 days to obtain a primary strain; transferring the primary strain to a liquid culture medium, wherein the liquid culture medium comprises the following components: 130 g of malt paste powder, 0.1 g of chloramphenicol and 1000 mL of water; and culturing in a constant-temperature shaking table at 28-30 DEG C at the speed of 150-200 revolutions per minute for 3-5 days to obtain a secondary strain. According to the multi-stage strain fermentation and sterilization packaging method for the red yeast rice, provided by the invention, the traditional method is low in strain activity and weak in reproductive capacity and directly restricts the fermentation efficiency due to extensive strain culture, and a culture medium formula and culture parameters are customized for strains in different stages through three-stage strain staged culture.

The invention belongs to the technical field of edible fungus preservation and cultivation, and particularly relates to an edible aerobic strain preservation method which comprises the following steps: S1, placing target hyphae in a solid culture medium for cultivation, then selecting the target hyphae with good growth vigor, and transferring the selected target hyphae to a culture medium slope of a sterile and nutrient-poor culture container for cultivation, stopping culturing when hyphae grow to 4 / 5 of the length of the inclined plane; s2, a covering solution is prepared, the covering solution comprises glycerin and paraffin oil, and the sterile preservation covering solution is applied to the culture container and completely submerges the slant of the culture medium; wherein the mass percentage of the glycerol is 60%-70%, and the mass percentage of the paraffin oil is 30%-40%; and S3, sealing the cultivation container by using a sealing element with controllable air permeability, and preserving in a dark place at normal temperature. Compared with the prior art, the technical problem that aerobic edible mushrooms cannot be well preserved by a paraffin oil method in the prior art is solved by setting the proportion of the glycerol to the paraffin oil.

The invention discloses an improved molasses culture medium and an application method of the improved molasses culture medium in increasing the yield of bird nest alkane diterpenoid high-activity compounds, and relates to the technical field of microbial fermentation. The invention aims to solve the problems of long production period, low product yield, large batch difference and difficulty in large-scale and process control when a solid culture medium is used for static fermentation in the existing production method. The method comprises the following steps: inoculating 0.1-0.5% of black egg nest fungus into a flat plate solid culture medium for culture, and then transferring into a liquid seed culture medium for shake culture to obtain a seed solution; inoculating the seed liquid into a fermentation tank filled with an improved molasses culture medium for deep fermentation to obtain fermentation liquid; mycelia are separated from the fermentation liquor, and a product rich in the bird nest alkane diterpenoids is obtained through washing, quick freezing, drying, smashing, extracting and concentrating. The invention can obtain the improved molasses culture medium and the application method of the molasses culture medium in increasing the yield of the bird's nest alkane diterpenoid high-activity compounds.

The invention belongs to the technical field of microbial fermentation, and discloses a method for screening coenzyme Q10 producing bacteria by using rhamnolipid, which comprises the following steps: coating a rhamnolipid-containing solid culture medium with rhodobacter sphaeroides, culturing, selecting single colonies, inoculating the single colonies into a rhamnolipid-containing fermentation culture solution as a seed solution, and culturing to obtain the coenzyme Q10 producing bacteria by using the rhamnolipid-containing fermentation culture solution. And then carrying out fermentation culture in a fermentation culture solution containing the rhamnolipid for 72 hours, collecting thalli, and measuring the content of the coenzyme Q10, so as to obtain the coenzyme Q10 high-yield strain. According to the method, the coenzyme Q10 production fungus rhodobacter sphaeroides is directionally screened, so that the strain of which the yield of coenzyme Q10 is remarkably increased can be efficiently obtained, the yield can be stably inherited, and the industrial requirements are met.

The invention discloses a panda staple food phyllostachys edulis microorganism obtaining method, and belongs to the technical field of microorganism identification. Comprising the following steps: S1, sampling; s2, microorganism enrichment: putting the bamboo leaf sample into a sterile plastic package bag, and adding a buffer solution to carry out vortex and ultrasonic treatment on the sample. After washing is finished, the bamboo leaves are taken out, liquid in the sterile plastic package bag is centrifuged, supernatant is discarded, thalli are collected, then a buffer solution is added for resuspending the thalli, then secondary centrifugation is carried out, finally supernatant is removed, resuspending and centrifuging are carried out again, bacterial sludge is collected, and cryopreservation is carried out at the temperature of-80 DEG C for standby application. S3, microbial culture: taking the bacterial sludge in the step S2, and culturing in a manner of combining solid culture plate culture and liquid shaking culture; bamboo leaf lixivium, plant hormone analogues and the like are added into the solid culture medium and the liquid culture medium; s4, microbial identification. According to the method, comprehensive analysis of phyllostachys edulis microorganisms from an original sample to a culture flora is realized, and the community diversity covered by an identification result is ensured.

This application provides a solid culture medium for the isolation and purification of thermophilic acidophilic bacteria, its preparation method, and its application. The solid culture medium is prepared by mixing a nutrient composition, a coagulant, and deionized water. The coagulant accounts for 9% to 50% of the solid culture medium by mass. The coagulant is a high-acyl gellan gum, containing 3 to 4 acyl groups. The concentration of the high-acyl gellan gum is 5 g / L to 30 g / L. This solid culture medium is made using high-acyl gellan gum. By adjusting the mass percentage of the high-acyl gellan gum, especially by limiting its concentration, the solid culture medium can remain stable for a long time under high temperature and high acid conditions, maintaining good gelation properties. This provides a stable high-temperature and high-acid growth environment for thermophilic acidophilic bacteria, thus facilitating the successful isolation and purification of these bacteria. Furthermore, high-acyl gellan gum is widely available and inexpensive, further reducing culture costs.

The invention discloses a phosphorus-solubilizing fungus obtaining and optimizing method, and belongs to the field of environment and the field of agricultural biotechnology. The method comprises the following steps: collecting a soil sample of Inner Mongolia Tin-Go Union; performing liquid culture on the soil sample, and performing gradient dilution under a sterile condition; the method comprises the following steps: sucking 100 [mu] L of bacterial solutions with different concentrations, coating the bacterial solutions on a PVK solid culture medium added with chloramphenicol and streptomycin, culturing, selecting single colonies with good growth, typical characteristics and large and remarkable phosphate solubilizing circle, continuously carrying out streak purification on the PVK solid culture medium to obtain a target strain pure culture, preserving and identifying to obtain the phosphate solubilizing fungi; optimizing culture conditions: taking glucose as a unique carbon source, wherein the concentration of the glucose is 15g / L; ammonium chloride is used as a sole nitrogen source, and the concentration of the ammonium chloride is 0.082 g / L; the culture temperature is 30 DEG C; the initial pH is 5; the phosphorus solubilizing amount of the phosphorus solubilizing fungi is 858.31 mg / L.

The invention discloses a kit for efficiently capturing fungal pathogen nucleic acid from soil as well as a preparation method and application of the kit. The preparation method comprises the following steps: the kit comprises a solid culture medium enrichment bar, a rapid lysis solution and a neutralization solution. After the enrichment bar is inserted into the soil to be detected, the pathogenic bacteria are enriched on the culture medium matrix of the enrichment bar by utilizing the isotropic growth of the pathogenic bacteria, and the target pathogenic bacteria are physically purified from the soil environment rich in inhibitors. According to the method, nucleic acid purification is not needed, rapid alkali lysis can be directly carried out on the enriched product, and the obtained nucleic acid lysis solution can be directly used as a detection template for high-sensitivity molecular detection. According to the method, the problem of interference of the soil inhibitor on molecular detection is fundamentally solved, high-difficulty soil nucleic acid extraction is simplified into high-efficiency rapid nucleic acid extraction, and a key solution is provided for field on-site detection.

The invention provides a helicobacter pylori culture medium, a culture method and application thereof, and relates to the technical field of microbiological culture.The helicobacter pylori culture medium comprises a basic culture medium and antibiotics, and when the basic culture medium is a brain heart infusion agar culture medium, the basic culture medium is a solid culture medium and is used for activation by adding defiberized sheep blood or goat serum; when the basic culture medium is a Brinell broth culture medium, the basic culture medium is a liquid culture medium, essential amino acid is provided by adding L-cysteine, and the basic culture medium is used as a reducing agent, so that enrichment culture of anaerobic bacteria is facilitated. Meanwhile, ferric pyrophosphate, L-cysteine and antibiotics are combined for use, so that rapid growth of the helicobacter pylori is promoted, pollution caused by infectious microbes is effectively reduced, and a more stable growth environment is provided for the helicobacter pylori; data show that when the culture medium is used for culturing helicobacter pylori, compared with a traditional solid culture method, the yield of collected wet thalli is increased by nearly three times, and the high survival rate can be kept while high-speed multiplication culture of helicobacter pylori can be achieved.

The application discloses a coating method and a coating system, and relates to the technical field of microbiology, and in particular to a coating method and a coating system for a solid culture medium surface. The coating method comprises the following steps: providing a container which carries a solid culture medium surface; applying horizontal oscillation to the container, so that the solid culture medium surface is in a horizontal oscillation state; and spraying a fluid to be coated onto the solid culture medium surface while keeping the solid culture medium surface in the horizontal oscillation state, the fluid being a bacterial solution containing microorganisms. When the fluid contacts the solid culture medium surface in the horizontal oscillation state, the fluid is dispersed under the action of the horizontal oscillation and adheres to different regions of the solid culture medium surface due to adhesion, so as to form isolated single clone colonies. The application can simultaneously meet the requirements of high-throughput automation adaptability and single clone colony distribution uniformity, and further improves the single clone colony acquisition rate.

The invention is applicable to the technical field of crude oil treatment, and provides a method for separating and screening crude oil degrading functional bacteria. Comprising the following steps: S1, collecting an environmental sample polluted by crude oil for a long time; s2, inoculating the environmental sample into a gradient carbon source inorganic salt enrichment culture medium for enrichment culture to obtain an enrichment culture solution; s3, performing gradient dilution on the enrichment culture solution, coating the enrichment culture solution on a selective solid culture medium containing crude oil, and culturing to obtain a single colony; s4, picking single colonies, inoculating the single colonies into a liquid culture medium containing crude oil, and determining the crude oil degradation rate of the single colonies; s5, screening out strains with the crude oil degradation rate higher than a preset threshold value, namely the crude oil degradation functional bacteria. According to the method, the crude oil is used as a unique carbon source, infectious microbes which can only utilize an easily degradable carbon source are eliminated from the source, rare strains which really have a crude oil-enzyme system are enriched, the abundance of target bacteria can be improved by 2-3 orders of magnitude through first passage, and the subsequent purification period is shortened by more than 50%.

The invention relates to the field of microbial screening, and particularly discloses a bacillus strain capable of reducing vegetable heavy metal accumulation and application, the technical scheme is that the method comprises the following steps: i, collecting a healthy plant root system in a cadmium polluted area, and preparing a root system attached bacterium suspension; ii, diluting the bacterial suspension in a gradient manner, and then coating the diluted bacterial suspension on an LB solid culture medium plate for purification to obtain a pure bacterial strain; and iii, inoculating the pure strains to LB solid culture medium plates containing different concentrations of cadmium, and screening out tolerant strains of which the bacterial colony diameter is not less than 2 mm on a 100 mg / L cadmium concentration plate and the bacterial colony diameter is not less than 1 mm on a 150 mg / L cadmium concentration plate. According to the preparation method, the bacterial colony growth diameter under the specific cadmium concentration gradient and the cadmium adsorption rate threshold value under the specific liquid culture condition are set as screening standards, and the bacillus strain with the high heavy metal adsorption and immobilization capacity is obtained.

The invention discloses a genetic engineering photosynthetic microorganism and application thereof in synthesis of L-lactic acid by using CO2, and belongs to the field of production of lactic acid by microorganisms. The gene engineering photosynthetic microorganism is synechococcus longisporus and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 2025896; a process for synthesizing L-lactic acid by using the gene engineering photosynthetic microorganism comprises the following steps: inoculating the gene engineering photosynthetic microorganism into a solid culture medium for culturing; the method comprises the following steps: inoculating genetic engineering photosynthetic microorganisms cultured in a solid culture medium into a liquid culture medium, and activating bacterial strains to prepare seeds; and inoculating the seeds into a flat plate type illumination reactor, and introducing CO2 gas in a liquid culture medium under an illumination condition for fermentation culture to prepare the L-lactic acid.