11 results about "Solid medium" patented technology

This invention discloses a culture medium for manganese-oxidizing bacteria suitable for low-carbon source environments in water supply networks and its application. The culture medium is prepared by mixing ferric ammonium citrate, manganese sulfate, ammonium sulfate, sodium chloride, magnesium sulfate, dipotassium hydrogen phosphate, and sodium nitrate, followed by high-temperature and high-pressure sterilization to obtain a culture solution. Manganese sulfate is added to the culture solution to obtain the manganese-oxidizing bacteria culture medium. Manganese-oxidizing bacteria are screened using this culture medium. Cast iron pipe scale is subcultured in the medium to enrich the manganese-oxidizing bacteria culture solution. The enriched manganese-oxidizing bacteria culture solution is diluted and spread onto agar solid medium, and incubated at a constant temperature to obtain manganese-oxidizing bacteria colonies. This invention solves the problem of altered bacterial physiological metabolism caused by high concentrations of added manganese ions, effectively improving bacterial activity and manganese oxidation capacity.

The application discloses a bacteriostatic type of liquor dreg containing Zhangzhou Bacillus GBW-NX and a preparation method and application thereof, and belongs to the technical field of microorganisms. The preservation number of the Zhangzhou Bacillus GBW-NX is CGMCC No. 27402. It is proved through experiments that the Zhangzhou Bacillus GBW-NX has the bacteriostatic effect on inhibiting Escherichia coli, Salmonella and Staphylococcus aureus. The Zhangzhou Bacillus GBW-NX is placed in a solid culture medium containing the liquor dreg to be fermented and enzymolyzed to prepare the bacteriostatic type of liquor dreg. The bacteriostatic substance of the bacteriostatic type of liquor dreg is a bacteriocin. It is also proved that the bacteriostatic type of liquor dreg can improve the intestinal function of ruminants including lactating cows, and can also improve the milk yield of the cows, thereby widening the application range of the Zhangzhou Bacillus GBW-NX, providing a new direction for the feeding of ruminants, and having important significance for the breeding of ruminants.

The application discloses a coating method and a coating system, and relates to the technical field of microbiology, and in particular to a coating method and a coating system for a solid culture medium surface. The coating method comprises the following steps: providing a container which carries a solid culture medium surface; applying horizontal oscillation to the container, so that the solid culture medium surface is in a horizontal oscillation state; and spraying a fluid to be coated onto the solid culture medium surface while keeping the solid culture medium surface in the horizontal oscillation state, the fluid being a bacterial solution containing microorganisms. When the fluid contacts the solid culture medium surface in the horizontal oscillation state, the fluid is dispersed under the action of the horizontal oscillation and adheres to different regions of the solid culture medium surface due to adhesion, so as to form isolated single clone colonies. The application can simultaneously meet the requirements of high-throughput automation adaptability and single clone colony distribution uniformity, and further improves the single clone colony acquisition rate.

The present application relates to a kind of plasmid for endowing bacillus to degrade skatole function and its application, belong to agricultural environment microorganism and genetic engineering technical field, to solve the existing skatole management high cost, easy to produce secondary pollution, part of degradation strain exists biological safety risk, and the genetic element of natural degradation strain is difficult to cross-strain application problem.The present application discloses the plasmid of nucleotide sequence as shown in SEQ ID NO.1, by the plasmid is transformed into bacillus competent cell, is cultivated in M9 solid medium containing skatole and positive clone detection obtains recombination bacillus, it is inoculated with 0.5% to 5% inoculation amount in the medium containing 0.05 to 0.2g / L skatole, under the condition of 30 to 37 DEG C, 120 to 250rpm 4 to 48h can be degraded skatole.The present application is mainly used for the skatole deodorization of breeding manure and the like scene, provides safe controllable, efficient and scalable biological technology scheme.

This invention discloses a selective solid blood culture medium for isolating Cryptococcus pyogenes and its application. The culture medium is a solid medium containing 15.0 g / L tryptone, 10.0 g / L yeast extract, 0.5 g / L L-cysteine, 5.0 g / L sodium chloride, and 15.0 g / L agar. After sterilization and cooling to 50°C, 5% (v / v) defibrinated sheep blood, 0.010 g / L vitamin K, 0.010 g / L heme chloride, and 0.010 g / L colistin B are added. This invention's culture medium selectively inhibits Gram-negative bacteria such as Escherichia coli by adding colistin B. Simultaneously, by optimizing the nitrogen source, adding key growth factors such as L-cysteine, vitamin K, and heme chloride, and using a simple anaerobic gas-generating bag culture method, it can significantly improve the isolation efficiency of Cryptococcus pyogenes from clinical samples, forming typical colonies within 24-48 hours, facilitating rapid pathogen isolation.

This invention provides a marine-derived glycomonad K1 strain, a method for producing dextran anhydrase, and its applications. The strain is a Gram-positive actinomycete. Colonies on a solid culture medium containing blue dextran exhibit the following characteristics: initially smooth surface, turning into a white, dry powder after three days of incubation; dried, with radially arranged edges; white on the front and black and opaque on the back. This invention also provides a method for producing dextran anhydrase using the aforementioned glycomonad K1 strain. The dextran anhydrase prepared using this method exhibits good thermal stability and strong pH tolerance, and can be widely used in the sugar, food, pharmaceutical, health, daily necessities, and chemical industries.

The present application relates to the field of microbial fermentation, and discloses a solid-state fermentation method for high-yield production of Monascus yellow pigment by Monascus purpureus. By regulating key process parameters such as fermentation temperature, gelatinization amount, fermentation time, inoculation amount, loading amount of solid medium for fermentation, and types of carbon source matrix, the present application realizes efficient directional biosynthesis and enrichment of Monascin (MS) and Ankaflavin (AK). The method discards the traditional vague color value index, establishes a quality standard with MS and AK specific content as the core, and promotes the product from a food coloring agent to a medicinal raw material. The target components are enriched by solid-state fermentation technology, and the extraction and purification steps are omitted, thereby greatly reducing the cost. The finished product is Monascus powder, which is consistent with the traditional application scene. The final product has the high MS / AK content, low MK content, and no citrinin, and meets the high-end medicinal raw material standard.

This application relates to the field of agricultural microbiology, and in particular to a potassium-solubilizing and growth-promoting bacterial strain adapted to high-altitude and arid environments and its application. The strain was isolated from sandy soil in a recovery phase following degradation in the Ruoergai Grassland in a high-altitude and cold region. It was identified as a *Comamonas* strain by 16S rDNA gene sequencing and phylogenetic analysis. After 7 days of shaking culture in potassium-solubilizing liquid medium at 30°C and 180 r / min, the effective potassium content in the fermentation broth was 14.41 mg / L. The strain can grow in LB medium at 12°C, remains viable after freezing, and can also grow in LB solid medium containing 800 mM mannitol at 30°C. The strain is classified as *Comamonas sp ZSK1*, with accession number CCTCC NO: M 20252437, deposited on November 3, 2025, at the China Center for Type Culture Collection. The strain described in this application solves the technical problem of existing potassium-solubilizing bacteria being functionally inactive and having low colonization rates due to limited or insufficient stress resistance.

This invention discloses a method for preparing a strain of Lactobacillus saccharidus and its application in the preservation of chilled fresh meat, including the following steps: Step (1) Initial screening: Take 1.0 g of environmental sample, add 9 mL of sterile physiological saline, shake at 37℃ and 200 r / min for 30 min, sonicate for 10 min, then serially dilute to 10⁻⁴-10⁻⁶, take 100 μL and spread it on MRS solid medium, incubate at 37℃ for 48 h, pick single colonies and purify 3 times; Step (2) Antibacterial screening: Use 8 strains of putrefactive bacteria such as Enterobacter and Escherichia coli as indicator bacteria. The preparation method of this Lactobacillus saccharidus strain is based on natural sources, ensuring high safety and making it a suitable alternative to chemical preservatives. It exhibits significant inhibitory effects on major spoilage bacteria (such as Pseudomonas and Enterobacteriaceae) and pathogenic bacteria in chilled meat. Its low-temperature resistance (4℃) and acid and alkali resistance make it suitable for the storage environment of chilled meat. After application, it can reduce the total bacterial count, TVB-N content, pH value, and drip loss rate, delay color changes, and effectively extend the shelf life of chilled meat.

This application relates to the field of agricultural microbiology, and in particular to a phosphorus-solubilizing and growth-promoting bacterial strain adapted to cold and arid environments and its application. The strain was isolated from soil in cold regions and identified as *Pantoea agglomerans* by 16S rDNA gene sequencing and phylogenetic analysis. It can grow in LB medium at 12°C, form visible colonies in LB solid medium containing 1200 mM mannitol, and exhibits phosphorus-solubilizing ability in NBRIP liquid medium. The strain is classified as *Pantoea agglomerans* ZSP3, with accession number CCTCC NO: M 20252438, deposited on November 3, 2025, at the China Center for Type Culture Collection. This application demonstrates the stable survival of Pantoea agglomerans strain isolated from soil in high-altitude and cold regions under the dual stress of low temperature and drought. This not only solves the problem of low colonization rate of strains under the dual stress of low temperature and drought, but also effectively improves the biomass and stress resistance of forage grasses in high-altitude and cold and arid environments by integrating phosphorus solubilization function and growth promotion mechanism.

This invention discloses a method for synthesizing bio-nano selenium using Beauveria bassiana, belonging to the field of nano-selenium synthesis technology. The method specifically includes the following steps: (1) activating and culturing Beauveria bassiana with accession number CGMCC 3.6914 in a liquid culture medium to obtain a spore suspension; (2) inoculating the spore suspension into a solid culture medium, simultaneously adding Na2SeO3 mother liquor, and then performing shaking culture. After the culture is completed, mixture a is obtained; (3) mixture a is separated, purified, and freeze-dried to obtain bio-nano selenium powder. The method for biosynthesizing nano-selenium in this invention utilizes Beauveria bassiana B26 (… Beauveria bassiana The metabolic activity of the substance reduces Na2SeO3 to nano-selenium, which has high synthesis efficiency, low cost and low pollution. The preparation process is simple and has great economic and environmental benefits.