116 results about "Solid medium" patented technology

The invention discloses an artificial cultivation method of xylaria fungus sclerotium, and relates to the field of sclerotium cultivation. The method comprises the following steps: collecting xylaria producing sclerotia, obtaining spores by adopting a spore separation method or obtaining core tissues by adopting a tissue separation method, inoculating the spores or the core tissues on an enriched PDA culture medium to be cultured into bacterial colonies, selecting hyphae with vigorous growth vigor at the edges of the bacterial colonies, and performing purification culture until a mother strain only containing a single strain is obtained; performing gene sequencing to identify the strain; propagating the mother strain to obtain an original strain, inoculating the original strain into a sterilized solid culture medium, culturing in a dark place to obtain a cultivated strain, placing the cultivated strain on soil, reserving a space for sclerotium development around the cultivated strain, culturing in the dark place for 33-90 days, and harvesting the sclerotium. The xylaria sclerotium with single germplasm can be cultivated on a large scale, and the problems of wild resource shortage and germplasm mixing are effectively solved.

The invention discloses a plant lactobacillus and application thereof, and belongs to the technical field of microorganisms. The plant lactobacillus 002P provided by the invention is a new strain, and is round, white, neat in edge, smooth in surface and soft in texture on an MRS solid culture medium; growth of bacteria can be remarkably inhibited, an inhibition effect on staphylococcus aureus, pseudomonas aeruginosa and escherichia coli is achieved, and the inhibition zone is 23.33-279.33 mm; the healing of infected wounds can be obviously promoted; the selenium conversion rate is excellent, and the maximum selenium conversion rate can reach 88%; the nano-selenium prepared from the plant lactobacillus 002P is spherical, has the particle size of 200-500nm, has excellent properties of resisting oxidation, relieving hepatotoxicity induced by saturated fatty acid, promoting expression of selenoprotein in a mouse body and reducing blood fat, and has a wide application prospect.

The invention discloses a method for microfluidic high-throughput screening of AKK bacteria. The method comprises the following steps: 1) preparing a microfluidic chip; 2) collecting fresh excrement of healthy volunteers, diluting, and adding a fluorescence labeled antibody to obtain a dispersion phase; and (3) preparing liquid drops by taking paraffin oil and Span-80 as a mobile phase, enabling the liquid drops to enter a liquid drop culture tank through an S-shaped liquid drop circulation channel, culturing, introducing the mobile phase, introducing the liquid drops in the liquid drop culture tank into a liquid drop collection tank, taking out, detecting a fluorescence signal, culturing the liquid drops with the fluorescence signal on the improved brain heart infusion broth solid culture medium, and obtaining the brain heart infusion broth. Obtaining a to-be-identified strain; 4, the strain to be identified serves as a template, PCR amplification is conducted, sequencing is conducted on an amplification product, BLAST sequence comparison is conducted on a sequencing result on an NCBI website, and the target strain AKK bacterium is screened out. The method has the advantages of being high in throughput, simple in experimental process and high in specificity, and the screening efficiency of the AKK bacterium can be improved.

The invention relates to the technical field of bioengineering, in particular to a method for promoting cordyceps sinensis to produce conidia. The invention provides a method for promoting cordyceps sinensis to produce conidia, which comprises the following steps: (1) carrying out dark culture on cordyceps sinensis on a primary solid culture medium to obtain mycelium; and (2) transferring the mycelium obtained in the step (1) to a secondary solid culture medium, and carrying out environmental stimulation culture until conidia are generated. The method is suitable for industrial production, has the advantages of simplicity and convenience in operation, controllable cost, high repeatability and the like, can effectively shorten the fermentation period, improves the inoculation efficiency and the yield of metabolites, and provides reliable technical support for large-scale artificial culture and deep development of cordyceps sinensis.

The present invention discloses a method and apparatus for screening strains with high substrate utilization efficiency using CO2 release rate, belonging to the field of microbial screening. The method comprises: standardized strain culture: inoculating the strain to be screened onto a solid culture medium, controlling environmental parameters and culturing in the dark; dynamic CO2 monitoring: collecting CO2 concentration using a CO2 sensor with integrated temperature, humidity, and pressure compensation; metabolic signature extraction: calculating the CO2 release rate per unit time, the release rate slope, and the normalized cumulative release of hyphae; hyphae growth monitoring and data synchronization: calculating the colony specific growth rate and generating a synchronized data set with CER data; hyphae growth metabolic correlation analysis: establishing a coupled kinetic model of hyphae growth and CO2 release; efficiency prediction and strain screening: using a multivariate regression model to predict substrate utilization efficiency and screening efficient strains according to grading criteria. The present invention has the advantages of being non-destructive and highly real-time, significantly shortening the screening cycle for strains with high substrate utilization efficiency.

To provide a high-hardness formulation used as a material containing microorganisms, and a method for producing the same. [Solution] The formulation according to the present disclosure comprises a solid culture containing a microorganism that forms mycelial masses and a solid medium on which the microorganisms are cultured. The formulation has a water content of 1 to 15% by weight. The formulation also has a hardness of 30 to 200 N. The manufacturing method of the formulation according to the present disclosure comprises step (1): drying the culture containing a microorganism that forms mycelial masses and a solid medium on which the microorganisms are cultured so that the water content is 1 to 15% by weight. The manufacturing method also comprises step (2): solidifying the dried culture.

The invention discloses mine water pseudomonas as well as a screening method and application thereof in recharge capacity increase, and belongs to the technical field of mine water recharge engineering. A mine water sample is taken back and then filtered through a filter membrane and a vacuum suction filter; after constant-temperature oscillation, inoculating a liquid LB meat extract peptone culture medium for enrichment culture; carrying out strain separation on the bacterium liquid subjected to enrichment culture by adopting a dilution coating plate method; the method comprises the following steps: carrying out streak culture on a single colony on a solid LB culture medium to obtain a pseudomonas colony, the pseudomonas is Pseudomonas sp. B2, the taxonomic name is Pseudomonas sp. B2, the preservation number is GDMCC No.66726, the preservation date is July 22, 2025, and the preservation unit is Guangdong Province Microbial Culture Collection Center, and the screened pseudomonas capable of producing carbonic anhydrase can effectively promote the dissolution of calcite and dolomite, and can be used for producing carbonic anhydrase. The recharge amount is increased, and the recharge efficiency is improved.

This invention discloses a culture medium for manganese-oxidizing bacteria suitable for low-carbon source environments in water supply networks and its application. The culture medium is prepared by mixing ferric ammonium citrate, manganese sulfate, ammonium sulfate, sodium chloride, magnesium sulfate, dipotassium hydrogen phosphate, and sodium nitrate, followed by high-temperature and high-pressure sterilization to obtain a culture solution. Manganese sulfate is added to the culture solution to obtain the manganese-oxidizing bacteria culture medium. Manganese-oxidizing bacteria are screened using this culture medium. Cast iron pipe scale is subcultured in the medium to enrich the manganese-oxidizing bacteria culture solution. The enriched manganese-oxidizing bacteria culture solution is diluted and spread onto agar solid medium, and incubated at a constant temperature to obtain manganese-oxidizing bacteria colonies. This invention solves the problem of altered bacterial physiological metabolism caused by high concentrations of added manganese ions, effectively improving bacterial activity and manganese oxidation capacity.

The invention discloses a method for culturing fungal-feeding nematodes by using two fungi in a mixed manner and reducing pollution of a culture medium. The method comprises the following steps: A, separating and extracting euphorbia fungal-feeding nematodes; b, identifying the euphorbia fungal nematodes; c, preparing a strain mixture which comprises botrytis cinerea and trichoderma harzianum in a mass ratio of 1: 1; d, inoculating the strain mixture into a PDA liquid culture medium to prepare a nematode PDA culture medium; e, centrifuging the nematodes obtained in the step B; f, disinfecting the nematodes, namely disinfecting the surfaces of the nematodes by using a mixed solution of streptomycin sulfate and actinomycete ketone; g, adding sterile water into the nematodes disinfected in the step F, and centrifuging; h, repeating the step G for multiple times to obtain nematode liquid; i, inoculating the disinfected nematodes into a flat plate of a PDA solid culture medium; and J, observing the growth quantity of the nematodes in the culture medium by using an inverted microscope. According to the method, the nematodes are cultured by culturing the fungus-eating nematode strain mixture, and the method is used for culturing and propagating the nematodes.

The invention provides a method for screening acid-producing strains, which comprises the following steps: 1) mutagenizing original strains, and constructing a mutant library; 2) primarily screening mutants in the mutant library by using a calcium carbonate transparent ring method or a pH indicator method to obtain primarily screened strains; (3) inoculating the primarily screened strains into a solid culture medium, culturing, selecting single colonies with the same diameter after the colonies grow out, punching the single colonies with the same diameter by using a punching device to obtain gel blocks of the solid culture medium with the same size or basically the same size, and culturing all the gel blocks under the same or basically the same condition to obtain gel blocks of the solid culture medium; recording the time when the gel block completely becomes transparent or changes color, and screening out secondary screening strains according to the time; 4, the secondary screening strains are subjected to fermentation culture, the yield of the organic acid is detected, and the acid-producing strains are screened out according to the yield of the organic acid.The method is simple and easy to operate, and the target strains can be more accurately and rapidly screened out.

This invention discloses a strain of *Bacillus parabrachium* ABX1 and its applications, belonging to the field of beneficial microbial technology. The *Bacillus parabrachium* ABX1 provided by this invention is a Gram-positive bacterium isolated from the intestinal fluid of *Bombyx mori*. On CMC-Na solid medium, the ratio of the diameter of the hydrolysis zone to the colony diameter can reach 11. Enzyme activity identification results show that the filter paper enzyme activity of *Bacillus parabrachium* ABX1 is as high as 109.79 U·mL. ‑1 The exo-β-glucanase activity was 107.59 U·mL. ‑1 The cellulase activity reached 107.37 U·mL ‑1 It exhibits excellent cellulose and / or crystalline cellulose degradation capabilities; it is easy to use and has a wide range of enzyme production conditions, showing excellent enzyme activity at pH 5–10 and 25–50℃; the enzyme production time is short, with high enzyme yield achieved in just 12 hours, and there is no significant adverse effect on enzyme yield with prolonged reaction time; it is highly safe to use and has great application prospects in areas such as oak garden waste branch treatment, improvement of oak silkworm digestibility, industrial degradation of cellulose, and improvement of soil fertility.

The invention provides a mycobacterium tuberculosis attenuated live vaccine strain solid culture medium as well as preparation and application thereof, and the raw material of the solid culture medium comprises supernate obtained by filtering potato water prepared from selenium-enriched yellow-core potatoes with the total selenium content of 15-150mu g / kg. A solid culture medium without animal-derived components is obtained; and the culture method is further optimized, so that the mycobacterium tuberculosis is high in thallus proliferation speed and not easy to be infected by infectious microbes when being cultured. The solid culture medium disclosed by the invention is simple in manufacturing process, low in implementation cost and high in conversion efficiency, and has a prominent popularization prospect.

The utility model discloses an improved culture bottle special for mycoplasma culture, and relates to the technical field of bioengineering. The bottle comprises a bottle body and a bottle cap, the inner side and the outer side of the periphery of a bottle opening in the upper side of the bottle body are respectively provided with a threaded structure, and the bottle body is transparent or semitransparent; the bottle cap is mounted on the bottle body through the bottle body outer side threads; a gas-permeable membrane assembly is arranged on the bottle cap, and the gas-permeable membrane assembly is composed of at least one layer of microporous filtering membrane and a supporting frame; an anti-adsorption coating is arranged on the inner wall surface of the bottle body; a solid culture medium is arranged in the bottom of the bottle body, the upper surface of the solid culture medium inclines to the bottle bottom, and a liquid culture medium is filled between the solid culture medium slope and the side wall of the bottle body; the anti-splashing device is installed in the bottle body through threads on the inner side of the bottle opening and is in an inverted circular truncated cone shape, and the interior of the anti-splashing device is hollow. By improving the material of the bottle body, optimizing the inner wall coating and arranging the breathable membrane assembly, the safety, reliability and visualization degree of the mycoplasma culture process are improved, and meanwhile, the risk of cross contamination is effectively reduced.

The invention discloses a morchella hypha powder prefabrication method and equipment line, belongs to the technical field of morchella hyphae, and solves the problems that morchella is extremely sensitive to continuous cropping, metabolite changes the soil microenvironment, hypha division and nutrient absorption are directly hindered, the hypha growth speed is reduced by 30%-50%, the biomass is reduced, and primordium formation is delayed. Comprising the following steps: S1, carrying out stress-resistant intensified culture on liquid strains; chitosan in a specific proportion in a liquid strain neutralizes dopamine quinone, antioxidant enzyme is induced to be improved, fat-soluble toxin is embedded in beta-cyclodextrin in a solid culture medium, dendrobium nobile residues adsorb water-soluble toxin, sodium selenite enhances oxidation resistance, germanium dioxide promotes hypha branching, an overburden filtrate degrades dopamine quinone, ash hydrolyzes related toxin, and the yield is increased. Pseudomonas compensates for beneficial metabolites and accelerates metabolism through temperature difference stimulation, so that hypha growth is accelerated, differential metabolites are reduced, biomass is increased, and the three-crop fruiting rate is stable.

The invention relates to a method for detecting microbial colonies in a biological sample placed in a solid medium by means of a detection system comprising at least one incubator, an analysis unit and at least one automatic image capture system, comprising a first stage, the present invention relates to a method for detecting and counting objects exhibiting growth in the field of view of an image capture system, and to a second stage applied only to those samples deemed negative in the first stage of the method, the second stage being based on the detection of anomalies on at least the last image acquired in a culture sequence.

The invention provides a bacillus high-yield strain for producing riboflavin and application of the bacillus high-yield strain, and belongs to the technical field of microbial fermentation. The bacillus strain is named as VB2-M-230602-66, the preservation unit is China Center for Type Culture Collection, the preservation date is December 1, 2023, and the preservation number is CCTCC NO: M20232421; according to the invention, the strain VB2-M-230602-66 with stable character is obtained by adopting physical mutagenesis separation and combining with shake flask screening of tolerance dose of a resistance marker, riboflavin can be produced through liquid fermentation, and the yield is stable; the bacterial strain has the biological characteristics that the bacterial strain is gram-positive bacteria through gram staining, the bacteria are cultured at 37 DEG C for 18 hours, the thalli are rod-shaped, 0.5-0.8 mu m * 2.0-5.4 mu m and are arranged in a single or paired manner, and bacterial colonies cultured on a solid culture medium are pale yellow, nearly circular, wet in surface, opaque and irregular in edge; the bacillus strain VB2-M-230602-66 provided by the invention is suitable for fermentation production of riboflavin (vitamin B2), has stable production performance, can obtain greater economic benefits, and is worthy of popularization.

The invention discloses a phosphate-solubilizing bacterium W047 with resistance to various heavy metals and application of the phosphate-solubilizing bacterium W047, the taxonomic name of the phosphate-solubilizing bacterium W047 is Burkholderia ambifaria, the phosphate-solubilizing bacterium W047 is preserved in the China General Microbiological Culture Collection Center on January 6, 2025, and the preservation number of the phosphate-solubilizing bacterium W047 is CGMCC No.33300. The phosphate-solubilizing bacterium W047 is obtained by directly separating and purifying arsenic-polluted farmland soil by taking a PVK solid culture medium as a basic culture medium, and has the characteristics of dissolving insoluble inorganic phosphorus (calcium phosphate, iron phosphate and aluminum phosphate), fixing nitrogen, producing iron carriers and the like. The phosphorus-solubilizing strain W047 can remarkably promote dissolution of insoluble phosphorus in phosphate ore, and the soluble phosphorus content in supernatant of phosphate ore slices and powder samples under the condition of shake flakes in a laboratory is 92.94 + / -0.94 mg / L and 173.36 + / -21.17 mg / L. The bacterial strain can activate phosphorus in soil, and compared with a contrast, the available phosphorus in the soil added with bacterial liquid of the bacterial strain is increased by 15.48 + / -2.85 mg / kg after the soil is cultured for 20 days. The multiple functions of the strain W047 enable the strain W047 to have strong viability, and the strain W047 has important significance in enriching strain resources, reducing production cost, developing microbial agents and the like.

The present application relates to a kind of culture medium preparation method for studying the influence of algal liquid secretion on arabidopsis growth, belong to the field of biotechnology.The method includes the preparation of solid medium, extraction of algal liquid secretion, preheating, four major steps of medium mixing.The liquid blue algae extracellular secretion is mixed into solid medium in specific method, the interference of microcystis aeruginosa liquid medium on experimental results is excluded by designing negative control, the "dose-effect" study of secretion to plant is carried out by adjusting the ratio of extracellular secretion and medium, the chemical stability of active component in extracellular secretion is inferred by designing stability experiment.The culture method designed in the application can be widely applied in other similar researches exploring the influence of microbial secretion on arabidopsis growth.

The present invention provides a formulation based on bacteria of the Bacillus genus that are capable of generating active sludges that degrade molecules present in water / soil and which can adapt to the environment to which they are exposed. The bacteria are inoculated in an organic substrate for the growth thereof, through an aerobic fermentation process in solid medium. The formulation can reduce the lipid, fat and protein content in wastewater, as well as eliminate bad odours and coliforms from same. It is therefore proposed for use as a bioremediation agent for wastewater from nejayote, blackwater and fat traps.

The application discloses a bacteriostatic type of liquor dreg containing Zhangzhou Bacillus GBW-NX and a preparation method and application thereof, and belongs to the technical field of microorganisms. The preservation number of the Zhangzhou Bacillus GBW-NX is CGMCC No. 27402. It is proved through experiments that the Zhangzhou Bacillus GBW-NX has the bacteriostatic effect on inhibiting Escherichia coli, Salmonella and Staphylococcus aureus. The Zhangzhou Bacillus GBW-NX is placed in a solid culture medium containing the liquor dreg to be fermented and enzymolyzed to prepare the bacteriostatic type of liquor dreg. The bacteriostatic substance of the bacteriostatic type of liquor dreg is a bacteriocin. It is also proved that the bacteriostatic type of liquor dreg can improve the intestinal function of ruminants including lactating cows, and can also improve the milk yield of the cows, thereby widening the application range of the Zhangzhou Bacillus GBW-NX, providing a new direction for the feeding of ruminants, and having important significance for the breeding of ruminants.

The invention relates to the technical field of bacteriostatic effect evaluation, in particular to a phyllostachys pubescens leaf volatile oil bacteriostatic effect multi-dimensional evaluation method which comprises the following steps: setting a control environment with phyllostachys pubescens leaf volatile oil and a control environment without phyllostachys pubescens leaf volatile oil in a solid culture medium or a biological membrane culture device, and controlling culture temperature and time parameters to evaluate the bacteriostatic effect of phyllostachys pubescens leaf volatile oil. The bacteria grow to form bacterial colonies or biological membranes, and visible light and near-infrared bands are selected by utilizing multispectral imaging equipment. A moso bamboo leaf volatile oil treatment and control environment is set in a solid culture medium or a biological membrane culture device, and multi-channel image data from visible light to a near-infrared band is captured by using a multispectral imaging device, so that the micromorphological change of a bacterial colony or a biological membrane is captured; the limitation that the traditional method only depends on naked eye observation or single wave band imaging is overcome, and the physiological state differences, such as thallus density, pigment generation and metabolite accumulation, reflected by different spectral information can be revealed.

The invention provides a PEG / CaCl2 mediated Penicillium citrinum genetic transformation method, and belongs to the technical field of microbial genetic transformation, and the method comprises the following steps: adding a target gene segment into Penicillium citrinum protoplast suspension, and carrying out an ice bath reaction; the target gene segment is obtained by taking pJET-hph-02944 plasmid as a template and carrying out PCR (Polymerase Chain Reaction) amplification by adopting a hygromycin B resistance gene primer pair with gene sequences as shown in SEQ ID No. 1 and SEQ ID No. 2; adding a PTC (Positive Temperature Coefficient) buffer solution into the mixed system, uniformly mixing and standing; continuously adding a TB3 liquid culture medium containing ampicillin, and carrying out recovery culture; uniformly mixing the resuscitated protoplast with a molten TB3 solid culture medium, pouring a plate, and carrying out dark culture for the first time; then covering a TB3 solid culture medium containing hygromycin B and ampicillin, carrying out secondary dark culture to obtain a transformant, and transferring the transformant to a PDA plate containing hygromycin B for screening. According to the method, CaCl2-PEG is adopted for mediated transformation of Penicillium citrinum, the transformation efficiency is higher, and the transformation speed is higher.

The invention relates to the technical field of food fermentation, in particular to a multi-stage strain fermentation and sterilization packaging method of red yeast rice. The method comprises the following steps that a high-quality monascus strain is selected and inoculated to a solid culture medium, the solid culture medium is prepared from 200 g of potatoes, 20 g of glucose, 15-20 g of agar and 1000 mL of tap water, and the pH value of the solid culture medium is 5.0-6.0; culturing in a constant-temperature incubator at 28-32 DEG C for 5-7 days to obtain a primary strain; transferring the primary strain to a liquid culture medium, wherein the liquid culture medium comprises the following components: 130 g of malt paste powder, 0.1 g of chloramphenicol and 1000 mL of water; and culturing in a constant-temperature shaking table at 28-30 DEG C at the speed of 150-200 revolutions per minute for 3-5 days to obtain a secondary strain. According to the multi-stage strain fermentation and sterilization packaging method for the red yeast rice, provided by the invention, the traditional method is low in strain activity and weak in reproductive capacity and directly restricts the fermentation efficiency due to extensive strain culture, and a culture medium formula and culture parameters are customized for strains in different stages through three-stage strain staged culture.

The invention discloses a bacterial drug resistance evolution tracking method based on a circular antibiotic concentration gradient plate, and belongs to the technical field of microorganisms. The method comprises the following specific steps: (1) pouring an antibiotic-containing LB solid culture medium into a circular culture dish, putting a circular-truncated-cone-shaped mold into the circular culture dish until the solid culture medium is solidified, and then taking out the solid culture medium, so that a concave structure inclined from the outside to the circle center is formed in the circular culture dish; (2) pouring an antibiotic-free LB solid culture medium into the sunken structure, and standing to obtain a circular antibiotic concentration gradient plate; (3) pouring a semi-solid culture medium into the circular culture dish, cooling and solidifying, dripping bacterial liquid to a central position, and culturing in an upright manner; and 4) acquiring a growth image of the bacterial colony at the same interval time, and judging the drug resistance evolution degree of the bacterial colony according to the outward diffusion rate of the bacterial colony from the circle center. The problems of fringe effect and uneven gradient distribution of a traditional rectangular culture dish are solved, and a new technical means is provided for bacterial drug resistance research.

The invention belongs to the technical field of edible mushroom genetic engineering, and relates to a method for constructing a mushroom genetic transformation system by using a G418 resistance selection marker, which comprises the following steps: taking a mushroom strain Shenxiang 215 cultured in a PDA (potato dextrose agar) culture medium as a receptor to obtain hypha blocks; the method comprises the following steps: transferring a pLgnpt plasmid into agrobacterium tumefaciens GV3101; agrobacterium tumefaciens is inoculated into an IM liquid culture medium containing acetosyringone and MES, hypha blocks are subjected to ultrasonic treatment and co-incubation with the hypha blocks, then the hypha blocks are transferred to an IM solid culture medium laid with filter paper, and dark culture is carried out; and transferring the hyphae subjected to dark culture to a PDA resistant plate culture medium containing G418 and Cef for resistance screening to obtain the shiitake transformant. The method not only verifies the applicability of the novel selection marker in flammulina velutipes, but also constructs a multi-marker technology platform parallel to the existing hygromycin system, and lays a foundation for subsequent complex operations such as multi-gene superposition editing, gene loop construction and the like.

The invention belongs to the technical field of edible fungus preservation and cultivation, and particularly relates to an edible aerobic strain preservation method which comprises the following steps: S1, placing target hyphae in a solid culture medium for cultivation, then selecting the target hyphae with good growth vigor, and transferring the selected target hyphae to a culture medium slope of a sterile and nutrient-poor culture container for cultivation, stopping culturing when hyphae grow to 4 / 5 of the length of the inclined plane; s2, a covering solution is prepared, the covering solution comprises glycerin and paraffin oil, and the sterile preservation covering solution is applied to the culture container and completely submerges the slant of the culture medium; wherein the mass percentage of the glycerol is 60%-70%, and the mass percentage of the paraffin oil is 30%-40%; and S3, sealing the cultivation container by using a sealing element with controllable air permeability, and preserving in a dark place at normal temperature. Compared with the prior art, the technical problem that aerobic edible mushrooms cannot be well preserved by a paraffin oil method in the prior art is solved by setting the proportion of the glycerol to the paraffin oil.

The invention discloses a dendrobium nobile endophytic fungus for producing dendrobine and application of the dendrobium nobile endophytic fungus. The method aims at solving the problems that in an existing plant, the dendrobine content is low, and the culture period is long. The dendrobium nobile endophytic fungus is fusarium 689, and is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 21, 202 5, and the preservation number is CGMCC 3.27824. The method for producing dendrobine by using the strain comprises the following steps: carrying out dark culture on preserved hyphae in an artificial climate box at 25 DEG C for 12 hours, then picking up the hyphae, inoculating the hyphae into a new solid PDA culture medium, carrying out dark culture at 25 DEG C for several days, picking up the hyphae, inoculating the hyphae into a 100ml liquid PDB culture medium, culturing in a culture bottle in a dark environment at the speed of 120 rpm and the temperature of 25 DEG C for about 30 days, and separating fermentation liquor from thalli (dried), so as to obtain the dendrobine. And detecting dendrobine from the thalli. The strain has good antioxidant enzyme activity and can also be applied to production of dendrobine through microbial fermentation.

The invention discloses an improved molasses culture medium and an application method of the improved molasses culture medium in increasing the yield of bird nest alkane diterpenoid high-activity compounds, and relates to the technical field of microbial fermentation. The invention aims to solve the problems of long production period, low product yield, large batch difference and difficulty in large-scale and process control when a solid culture medium is used for static fermentation in the existing production method. The method comprises the following steps: inoculating 0.1-0.5% of black egg nest fungus into a flat plate solid culture medium for culture, and then transferring into a liquid seed culture medium for shake culture to obtain a seed solution; inoculating the seed liquid into a fermentation tank filled with an improved molasses culture medium for deep fermentation to obtain fermentation liquid; mycelia are separated from the fermentation liquor, and a product rich in the bird nest alkane diterpenoids is obtained through washing, quick freezing, drying, smashing, extracting and concentrating. The invention can obtain the improved molasses culture medium and the application method of the molasses culture medium in increasing the yield of the bird's nest alkane diterpenoid high-activity compounds.

The invention belongs to the technical field of microbial fermentation, and discloses a method for screening coenzyme Q10 producing bacteria by using rhamnolipid, which comprises the following steps: coating a rhamnolipid-containing solid culture medium with rhodobacter sphaeroides, culturing, selecting single colonies, inoculating the single colonies into a rhamnolipid-containing fermentation culture solution as a seed solution, and culturing to obtain the coenzyme Q10 producing bacteria by using the rhamnolipid-containing fermentation culture solution. And then carrying out fermentation culture in a fermentation culture solution containing the rhamnolipid for 72 hours, collecting thalli, and measuring the content of the coenzyme Q10, so as to obtain the coenzyme Q10 high-yield strain. According to the method, the coenzyme Q10 production fungus rhodobacter sphaeroides is directionally screened, so that the strain of which the yield of coenzyme Q10 is remarkably increased can be efficiently obtained, the yield can be stably inherited, and the industrial requirements are met.

The invention discloses a method and a device for screening strains with high matrix utilization efficiency by utilizing a CO2 release rate, and belongs to the field of microbial screening. The method comprises the following steps: standardized culture of strains: inoculating to-be-screened strains into a solid culture medium, controlling environmental parameters, and culturing in a dark place; cO2 dynamic monitoring: collecting CO2 concentration by using a CO2 sensor integrated with temperature, humidity and pressure compensation; metabolic characteristic extraction: calculating a CO2 release rate per unit time, a release rate slope and a hypha normalized accumulative release amount; hypha growth monitoring and data synchronization: calculating a bacterial colony specific growth rate, and generating a synchronous data set with CER data; hypha growth and metabolism correlation analysis: establishing a coupling kinetic model of hypha growth and CO2 release; efficiency prediction and strain screening: predicting matrix utilization efficiency by using a multivariable regression model, and screening high-efficiency strains according to grading standards. The method has the advantages of being non-destructive, high in real-time performance and the like, and the screening period of strains with high matrix utilization efficiency is remarkably shortened.