326 results about "Potato dextrose agar" patented technology

The invention relates to a method for jointly treating stalks by steam explosion and microorganism fermentation, comprising the following steps of: firstly, carrying out steam explosion pretreatment on the stalks to obtain exploded stalks; then preparing spore seed liquid: inoculating aspergillus oryzae or trichoderma Koningi to a PDA (Potato Dextrose Agar) culture medium, standing and culturing for 3-5 days at the temperature of 28-32 DEG C, and preparing the spore seed liquid with the concentration of 106-108 per mL; and finally carrying out microorganism fermentation: uniformly mixing 18g of exploded stalks, 2g of bran and 30mL of mineral element nutrient solution, adjusting pH to be 7.0 with Ca(OH)2, sterilizing for 15min at the temperature of 121 DEG C and the pressure of 0.15MPa to obtain a solid fermentation medium a, inoculating the spore seed liquid according to 2-4% of inoculum size, and culturing the spore seed liquid for 5-7 days at the temperature of 28-32 DEG C. The fermentation stalks obtained by utilizing the method have low content of lignose, cellulose and hemicellulose and high activity of filter paper carbohydrase, CMC (carboxymethyl cellulose) enzyme, amylase and protease.

A process and method for the production of endophytes as plant inoculant products, specifically Clonostachys rosea strain 88-710, for the promotion of plant vigor, health, growth and yield are disclosed. The endophyte, Clonostachys rosea strain 88-710 produces a fungal conidial preparation by utilizing a discrete solid substrate fermentation system, namely Potato Dextrose Agar or Malt Extract Agar. Additionally, the endophyte, Clonostachys rosea strain 88-710, can act as an inoculant to stimulate and have an additive effect with rhizobium bacteria on the production of nitrogen fixing nodules on legumes and growth enhancement e.g. beans, soybeans, peas and alfalfa. As well, Clonostachys rosea strain 88-710, can combine with rooting hormones, e.g. indole-3-butyric acid (IBA) to provide inoculant and rooting benefits to cuttings/transplants of plants.

The invention relates to a preparation method of trichoderma multifunctional soil modifying agents. The preparation method comprises the steps that: trichoderma harzianum SH2303 with the CGMCC (China General Microbiological Culture Collection Center) NO. 4963 is cultured on a potato dextrose agar (PDA) culture medium for 2 to 3 days, a bacterial cake is punched by a hole puncher with the diameter being 5mm, the bacterial cake is inoculated into a seed culture medium for 5 to 7 days at the bacterium inoculation quantity of three tablets per bottle and is then inoculated into a spore production culture medium, the fermentation is carried out for 5 to 7 days under the optimized culture condition in a 300 L fermentation tank, and the number of thick-wall spores is (6 to 7)*10<8>/mL; and fermenting liquid and carriers are mixed according to the weight ratio of 1:1, the secondary open type solid fermentation is carried out for 5 to 7 days at the normal temperature, and the number of trichoderma conidiospores and chlamydospores is 200 to 500 million/g. The soil modifying agents prepared by the method have the main purposes of improving the primary and secondary salinization of vegetable planting soil and degrading the organic phosphorus pesticide residue soil, and have the effects of treating soil-borne diseases, promoting the crop growth and improving the crop yield. Because only one trichoderma strain is utilized, the fermentation process is easy to optimize, the microbial inoculum preparation cost is low, the commercial large-scale popularization is easy to realize, and high field general use values are reached.

The invention discloses a chaetomium globosum FSR-74. The collection unit of the chaetomium globosum FSR-74 is China General Microbiological Culture Collection Center, the collection date of the chaetomium globosum FSR-74 is September 19, 2014 and the collection number of the chaetomium globosum FSR-74 is CGMCC No. 9690. The strain is relatively slow in growth and has light brown colonies and an irregular wave-like edge when subjected to plate culture at 25 DEG C in a potato dextrose agar (PDA) culture medium. FSR-74 ascocarps are supergene and fixed on the surface of a matrix by rhizoids; top hair is wavy, brown and has intervals, and dense wart points are arranged on the surface; 8 ascospores are arranged in an ascus; the ascospores are shaped like lemons and have single apical bud holes. The chaetomium globosum FSR-74 has an efficient broad-spectrum bacteriostatic effect against main pathogenic bacteria causing ginseng rust rot, blight, sclerotinia rot, rust rot, blackspot, sheath blight and gray mold. The invention further provides a biological control mechanism and application of the chaetomium globosum FSR-74 in prevention and treatment of plant fungal diseases, as well as application in preparation of a microbial agent for preventing and treating the plant fungal diseases.

The invention discloses an edible mushroom culturing method which comprises the following steps: inoculating edible mushroom strains to a solid medium and culturing for 25-40 days at the temperature of 20-26 DEG C, thus obtaining solid strains; adding a stabilizing agent into water or a PDA Potato Dextrose Agar fluid nutrient medium and mixing, thus obtaining a liquefying agent; adding the solid strains into the liquefying agent, stirring, filtering and taking filtrate, thus obtaining liquid strains; and inoculating the liquid strains into the production culture medium for production and cultivation, thus obtaining the edible mushroom. During the edible mushroom culturing process, the liquefying agent is utilized for liquefying the solid strains which are then inoculated for production and cultivation, the edible mushroom is produced for use, the operation is simple and convenient, the labor intensity can be lightened, the strain quantity can be well controlled, and standardized production is benefited; the product quality of scattered growers is stable, and regional scaled production can be formed; and simultaneously pollution can be effectively controlled, labor and material wastes can be reduced, and the product benefit and the product quality can be improved.

The invention discloses a polysporus trichoderma (Hypocrea pachybasioide) FSR-97 strain, wherein the preservation unit is China General Microbiological Culture Collection Center, the preservation date is 19, September, 2014 and the preservation number is CGMCC No.9691. The strain is cultured on a potato dextrose agar culture medium (PDA) plate at 25 DEG C and rapidly grows, and a monospore is germinated to form a white bacterial colony; the colour on the back surface of the bacterial colony is transited from colourless to yellow. The polysporus trichoderma (Hypocrea pachybasioide) FSR-97 has an effective broad-spectrum antibacterial effect on the main pathogenic bacteria causing the panaxginsen fusarium rot, epidemic disease, sclerotiniose, root rust rot, black spot, rhizoctonia solani, and botrytis. The invention further discloses a plurality of biocontrol mechanisms of the polysporus trichoderma (Hypocrea pachybasioide) FSR-97 for preventing and controlling the plant fungous disease, and an application for preparing a microbial preparation to prevent and control the plant fungous disease.

A de-bonding enzyme produced by Aspergillus niger HYA4 and its use in flax de-gumming are disclosed. The process is carried out by taking potato glucose agar culturing medium as tapered-land preservative culturing medium, taking solid fermenting culture medium as bran, bean dregs, ammonia sulfate and ammonium nitrate, putting ferment under room temperature, leaching by natural water stilly, filtering to obtain coarse enzyme liquid, diluting, and determining pectase and xylanase activity. It's cheap and non-toxic; has more enzyme yield, complete varieties, shorter production period, various raw material sources, more yielding rate and combing rate and less water-source pollution.

The invention provides a method for improving a submerged fermentation level of trichoderma reesei cellulase liquid. The method provided by the invention comprises the following steps of: first-grade seed culturing: preparing a spore suspension solution from activated trichoderma reesei CICC 13052 bacterial strain on a PDA (Potato-Dextrose-Agar) slant culture medium and inoculating the spore suspension solution into a fresh seed culture medium according to the inoculating amount of 3% to be cultured; and fermenting and culturing: inoculating the first-grade seeds in a logarithm growing periodinto a fresh fermenting culture medium to be cultured and controlling the pH values and dissolved oxygen in a pot of the fermenting process by sections to maintain the pH values and dissolved oxygen of the different periods at the different levels, and carrying out feed supplement and controlled culturing. By utilizing the method provided by the invention, the liquid is fermented for 168 hours; the enzyme activity of the fermenting liquid is measured by utilizing an international standard method which is recommended by the IUPAC (International Union of Pure and Applied Chemistry); and the enzyme activity of filter paper is 14.2 IU/mL and is improved by 67.1% compared with the interval fermenting enzyme activity level (8.5 IU/mL) before the process is optimized.

The invention discloses a separation and cultivation method for isaria fumosorosea. The method comprises the following steps of collecting bemisia tabaci bodies infected with entomogenous fungus from vegetable leaves, separating the entomogenous fungus from the bodies, putting the entomogenous fungus on a PDA (potato dextrose agar) culture medium for cultivating to obtain spore suspension which is sprayed on two-year-old nymphs of bemisia tabaci, then separating the entomogenous fungus from the bodies again after cultivation, storing the entomogenous fungus on a slope of a test tube and storing the test tube in a refrigerator; taking the entomogenous fungus out of the refrigerator, preparing the spore suspension, dipping the spore suspension through filtration paper, inoculating the spore suspension onto the culture medium, cultivating in a mildew incubator, picking up hypha, inoculating the hypha into germfree water, then stirring and filtering; putting the spore suspension at the center of a culture dish, perforating a hole in the center of a bacterial colony, dispersing tween-80, stirring and filtering again, and calculating the sporulation quantity and the diameter of the bacterial colony. The method is used for screening an optimal culture medium formula, the optimal temperature, an optimal pH, a lighting condition and a ventilating condition. The method has the advantages that the diameter area of the bacterial colony is large and the sporulation yield is large.

The invention provides a production method of a liquid phagostimulant for fattening pigs. The production method is characterized by comprising the following specific steps of: performing streak culture of Saccharomyces boulardii and Lactococcus lactis; inoculating Saccharomyces boulardii and Lactococcus lactis into a potato dextrose agar (PDA) culture medium and an M17 liquid culture medium for culturing to obtain Saccharomyces boulardii liquid and Lactococcus lactis liquid; inoculating the Saccharomyces boulardii liquid into a Saccharomyces boulardii fermentation broth culture medium for aerobic culture to obtain Saccharomyces boulardii fermentation broth, and inoculating the Lactococcus lactis liquid into a Lactococcus lactis fermentation broth culture medium for anaerobic culture to obtain Lactococcus lactis fermentation broth; and mixing the Saccharomyces boulardii fermentation broth and the Lactococcus lactis fermentation broth to obtain the liquid phagostimulant. The raw materials used in the liquid phagostimulant provided by the invention are all natural agricultural products and food raw materials. The liquid phagostimulant is prepared by a simple production method, has good phagostimulant effect and functions of increasing feed intake and saving the investment in high-price feed.

The invention relates to a method for artificially cultivating Ganoderma guinanense fruiting bodies, which is characterized by comprising the following steps of: carrying out a tissue separation or a spore separation on fresh Ganoderma guinanense (Ganoderma guinanense J.D.Zhao et Z.Q.Zhang) fruiting bodies; then culturing on a PDA (Potato Dextrose Agar) slant culture medium and a slant comprehensive PDA culture medium to obtain a stock spawn for production; then inoculating the cultured sporophores into a stock culture medium to be cultured; culturing until mature fruiting bodies grow. According to the artificially cultivating method provided by the invention, the biological efficiency of the Ganoderma guinanense fruiting bodies can reach 35-75%, namely 35-75 g of fresh Ganoderma guinanense fruiting bodies can be obtained by utilizing 100 g of culture materials for production; and the fresh Ganoderma guinanense fruiting bodies can be used as raw materials or materials for researching.

The invention aims at providing a preparation method of a Phellinus linteus mycelium. The method comprises the following steps of: activating Phellinus linteus spawn; executing plate cultivation of potato dextrose agar culture medium; scraping the Phellinus linteus mycelium to inoculate the scraped Phellinus linteus mycelium to the potato dextrose agar culture medium for shake cultivation; collecting the Phellinus linteus mycelium; extracting Phellinus linteus mycelium total RNA; and observing result via agarose gel electrophoresis. The spawn used in the preparation method is the Phellinus linteus spawn (Phellinus baumii Pilate) collected in the CCTCC (China Center for Type Culture Collection), and the collection number is Phellinus linteus DL101 CCTCC M 2011137. The preparation method is simple to operate and has straightforward principle, and a simple and convenient method for obtaining large-scale high-purity Phellinus linteus mycelium and high-quality Phellinus linteus total RNA in the future is provided, and the basis for fundamental research and application development of the Phellinus linteus is also provided.

The invention discloses a method for producing cordycepin through semi-continuous liquid fermentation of cordyceps militaris, and the method comprises the following concrete operating steps of: (1) putting hyphae of the Cordyceps militaris on a PDA (Potato Dextrose Agar) solid culture medium and activating to generate a bacterial lawn with a plenty of dense mycelia; (2) putting the bacterial lawn on a seed culture medium, carrying out shake cultivation, and forming a plenty of mycelium pellets after 5 days, thereby obtaining seed liquid; (3) transferring the seed liquid into a seed liquid fermentation tank to carry out expanding culture; (4) transferring the seed liquid after being subjected into the expanding culture in a 500L tank to carry out first-batch fermentation; and (5) carrying out semi-continuous fermentation in 10 batches in the 500L tank under the same conditions. In the fermentation process, a method of adjusting the culture medium composition and the fermentation conditions is adopted to control the cordyceps militaris to form the mycelium pellets with regular shape and uniform size and the average diameter of the mycelium pellets is 3-5mm, so that the separation of fermentation broth and thalli is easy; the carbon and nitrogen source and other inorganic salts needed in the growth of mycelium pellets are saved by utilizing the repeated fermentation of the mycelium pellets of the cordyceps militaris, so that the cost is saved; and the fermentation cycle is shortened and the fermentation intensity is increased, so that the method is suitable for industrial production.

The invention provides a method for asexual propagation of wild aweto fungi and belongs to the technical field of biology. In the method, living cells of wild aweto are used as strains, and the aweto fungi suitable for artificial culture are obtained in a special potato dextrose agar (PDA) culture medium by a cloning technology; and the PDA culture medium comprises the following components by mass: 950 to 1,050 grams of water, 280 to 320 grams of potatoes, 4 to 8 grams of peptone, 3 to 5 grams of potassium dihydrogen phosphate, 1 to 2 grams of magnesium sulfate, 15 to 20 grams of agar and 18 to 23 grams of glucose, and the pH value is between 6.0 and 7.0. The method comprises the following steps: 1, selection of living cells; 2, domestication of strains; 3, purification; and 4, rejuvenation and obtaining of excellent mother seeds of aweto. The method has the advantages of reasonable nutrients of the PDA strain culture medium, high strain activity, no pollution by molds and bacteria, stable quality of fungi and no mutation of fungi. In addition, the content of effective nutrients of the aweto is high, and the good-quality and high-yield fruit body is obtained.

The invention provides a preparation method for multifunctional soil remediation microbial inoculum. The preparation method comprises the following steps: A. cultivating slope culture aspergillus niger strains J6 through a PDA (potato dextrose agar) culture medium until spores are mature, inoculating in a seed culture medium to obtain spore seed liquid after spore germination; and B. inoculating the spore seed liquid in a solid fermentation medium according to mass percent of 5-10%, conducting shallow pan cultivation for 4-7 days at the temperature of 25-35 DEG C, and turning over for 2-3 times to obtain the multifunctional soil remediation microbial inoculum. With the adoption of the multifunctional soil remediation microbial inoculum prepared by the method, organophosphorus pesticide in soil can be efficiently degraded, and heavy metal such as chromium and lead and the like in the soil can be efficiently adsorbed, so that the purpose of multiple remediation of the soil is achieved.

The invention discloses a morchella esculenta bacterial strain and a culture method thereof. The culture method of the morchella esculenta M-02# bacterial strain with a preservation number of CGMCC (China General Microbiological Culture Collection Center ) No.7058 is as follows: inoculating a mycelium of the morchella esculenta bacterial strain into a test tube culture medium inclined surface which contains an improved PDA (Potato Dextrose Agar) culture medium for culturing for 5 days-7 days under 18 DEG C-24 DEG C to obtain a test tube strain; inoculating the test tube strain into a triangular flask which contains a liquid culture medium for culturing on a shaking bed at 20 DEG C-26 DEG C, and culturing for 5 days-7days at rotation speed of 120 r/minute-160r/minute to obtain a primary liquid strain; and inoculating the primary liquid strain into a fermentation tank, ventilating for culturing for 72 hours-96 hours to obtain a liquid strain of the bacterial strain. The bacterial strain disclosed by the invention has mycelium biomass live-weight as high as 25g/kg, strong sclerotium generating capacity, pollution resistance, a low culturing contamination rate not greater than 2%, and short liquid fermentation time of 72 hours-96 hours, so that an excellent strain is provided for morchella esculenta wild resource protection and reproduction promotion.

The invention discloses a high-selenium liquid culture method for pleurotus eryngii. The method comprises the following steps of: (1) inoculating pleurotus eryngii strains into a PDA (Potato Dextrose Agar) comprehensive culture medium to perform test tube strain culture to obtain initial flora suspension; (2) mixing a selenium source and the PDA comprehensive culture medium to prepare selenium-containing culture mediums with gradient selenium concentrations respectively; sterilizing the selenium-containing culture mediums; inoculating the pleurotus eryngii strains into the sterilized selenium-containing culture mediums in a flora suspension inoculation mode to perform selenium-tolerant culture at the selenium concentrations one by one in the conventional pleurotus eryngii strain culture mode in an order from low to high of the selenium concentration, wherein the flora suspension used in the primary selenium-tolerant culture is initial flora suspension, the flora suspension adopted by the subsequent selenium-tolerant culture is the flora suspension used for performing the selenium-tolerant culture at the current selenium concentration, and the like; and the best selenium tolerance concentration of the pleurotus eryngii and the highly selenium-tolerant pleurotus eryngii strains are obtained; and (3) performing fermentative culture on the highly selenium-tolerant pleurotus eryngii strains obtained by the step (2). After the highly selenium-tolerant culture of the pleurotus eryngii strains, the tolerance against selenium content in a culture medium is greatly improved in the state of liquid submerged culture.

The invention belongs to the technical field of plant protection, and particularly relates to an efficient stable indoor inoculation method for Ustilaginoidea virens and a special strain. The method includes: subjecting a hyphal mass (6mm) cultivated for 7 days on PSA (potato dextrose agar) to shake cultivation through PSB (photosynthetic bacteria) for 7-10 days so as to prepare hypha and spore mixed liquor, and filtering the mixed liquor with four layers of filter cloth to obtain spore liquor inoculants 106/ml; inoculating rice ear bracts by injecting at the seventh stage of rice young ear differentiation; and keeping moist for three days at 25 DEG C and under 95%RH (relative humidity), and placing in a spray irrigation net chamber for cultivation at 25-32 DEG C and under 90%-98%RH. By the method, the rate of diseased ears under artificial inoculation of Ustilaginoidea virens can reach more than 90%. By the method compared with the outdoor artificial inoculation techniques, efficientstable incidence of Ustilaginoidea virens can be obtained under controllable conditions.

The invention relates to a method for preparing fibrilia by using epicoccum nigrum DB3 strains. The method comprises the following steps: (1) preserving the epicoccum nigrum DB3 strains in a potato dextrose agar medium for cultivation, and adding a freezing buffer solution; (2) inoculating bacteria the amount of which equals to the pick-up amount of an inoculating ring, obtained in the step (1) into the potato dextrose agar medium, and carrying out shaking culture; (3) inoculating the culture medium obtained in the step (2) into a flax lignin fermentation medium, and carrying out shaking culture to obtain a fermentation bacteria liquid; (4) mixing sterilized fiber raw material with the fermentation bacteria liquid obtain in the step (3), carrying out table shaking and removing fermentation liquid to obtain the fibrilia; and (5) mixing the fibrilia with degumming liquid, processing and then rinsing, oiling and drying to obtain the fibrilia. The method in the invention has simple technology, short degumming time and high degumming efficiency, thus being suitable for large-scale industrial production.

The invention discloses bacillus pumilus with a collection name of Bacillus pumilus 223, a collection unit of Chinese Type Culture Collection Center, a collection address of Wuhan University in Wuhan China, a collection date of April 8, 2010 and a collection number of CCTCC NO: M2010075. The invention also discloses a culture method of the bacillus pumilus and a method for preparing an antibiotic active substance by using the bacillus pumilus, comprising the following steps of: (1), carrying out streak culture on bacterial strains on a PDA (Potato Dextrose Agar) culture medium; (2), culturing a single colony in an SMA (Standard Methods Agar) fluid culture medium; (3), culturing bacterial liquid after being coated on an SMA solid culture medium; (4), soaking an obtained substance of the step (3) by using phosphate buffer and centrifuging; and (5) adding (NH4)2SO4 into supernatant liquid, centrifuging after standing and redissolving obtained precipitates by using the phosphate buffer. In the invention, the bacillus pumilus and the antibiotic active substance can be used for preventing and treating rice sheath blight diseases.

The invention provides a fungus strain A. flavus G10 for degrading polyurethane plastic. The microorganism preservation number of the fungus strain A. flavus G10 is GDMCC 60537. The invention also provides a method for culturing the fungus strain A. flavus G10. The method includes the steps of isolating the fungus strain from the intestinal tract of a cricket; adopting PU as a unique carbon sourcefor culturing the fungus strain in a liquid culture medium to obtain a culture solution; diluting the culture solution and coating a solidified nutrient agar culture medium containing tetracycline antibiotics and a potato glucose agar culture medium with the culture solution to obtain cultured growing products. The cultured growing products are subcultured on fresh plates at 30 DEG C until a single fungus strain is obtained on each plate. The fungus strain A. flavus G10 can degrade the polyurethane plastic quickly.

The invention provides a method for preparing active phenolic compounds through rosin-induced bioconversion. The method comprises the following steps: performing the routine culture of the microbial strain A5<+> in potato dextrose agar (PDA) culture medium to obtain the activated strain, inoculating the activated strain in the PDA culture medium to ferment for 48-72 hours; adding the inducer in the fermented strain to perform bioconversion; filtering and concentrating the conversion fluid to obtain conversion product crude extract; performing gradient alcohol precipitation on the conversion product crude extract, extracting with petroleum ether and ethyl acetate, finally separating and purifying the product through thin layer chromatography, silica gel column chromatography LH-20 gel chromatography and high performance liquid chromatography to obtain O,O-1,1'-di(2-hydroxyl-3,4,5-trimethyl)phenyl oxo bridge and 4,5,6-trimethyl-1,2-pyrocatechol. The bioconversion method has the advantages of high efficiency and selectivity and mild reaction conditions, and can hardly cause environmental pollution; the cost of the method is lower than the extraction method, thus the cost can be saved; and the obtained phenolic compounds have the functions of antioxidant property, antibacterial property, antiviral property, anticancer property, radiation resistance and antiallergic property.

The invention relates to a conventional low-temperature preservation method of a volvariella volvacea strain. The preservation method comprises the following steps: (1) transferring the volvariella volvacea strain onto a PDA (potato dextrose agar) plate, performing static culturing in an incubator at 30-32 DEG C, then transferring the strain to a PDA test tube slant after mycelia permeate the plate, performing static culturing at 30-32 DEG C for 3-7d; (2) preparing a mannitol solution which is 5-20% in mass fraction, and then filtering and sterilizing; and (3) transferring the mannitol solution in the step (2) to the test tube slant which is full of the mycelia in the step (1) by virtue of a sterile injector, and preserving the test tube slant at conventional low temperature. The preservation method disclosed by the invention can prolong a preservation duration of the volvariella volvacea mycelia in a low-temperature environment of 3-6 DEG C; the preservation method has the advantages of simple and convenient operation, low cost, long preservation duration of the strain, rapid recovery and growth, good stability and the like; and the preservation method is especially suitable for small and medium-sized enterprises and farmers planting the volvariella volvacea.