9 results about "Potato dextrose agar" patented technology

The invention relates to the technical field of food fermentation, in particular to a multi-stage strain fermentation and sterilization packaging method of red yeast rice. The method comprises the following steps that a high-quality monascus strain is selected and inoculated to a solid culture medium, the solid culture medium is prepared from 200 g of potatoes, 20 g of glucose, 15-20 g of agar and 1000 mL of tap water, and the pH value of the solid culture medium is 5.0-6.0; culturing in a constant-temperature incubator at 28-32 DEG C for 5-7 days to obtain a primary strain; transferring the primary strain to a liquid culture medium, wherein the liquid culture medium comprises the following components: 130 g of malt paste powder, 0.1 g of chloramphenicol and 1000 mL of water; and culturing in a constant-temperature shaking table at 28-30 DEG C at the speed of 150-200 revolutions per minute for 3-5 days to obtain a secondary strain. According to the multi-stage strain fermentation and sterilization packaging method for the red yeast rice, provided by the invention, the traditional method is low in strain activity and weak in reproductive capacity and directly restricts the fermentation efficiency due to extensive strain culture, and a culture medium formula and culture parameters are customized for strains in different stages through three-stage strain staged culture.

The present application belongs to the technical field of contaminated soil remediation, and relates to an ecological activation remediation material for heavy metal contaminated soil and a preparation method thereof, comprising: obtaining biochar by anaerobic pyrolysis, crushing and sieving of corn stalks; obtaining modified biochar wet material by dispersing the biochar in a modified solution, mixing, stirring and heating; obtaining a bacterial suspension by inoculating Burkholderia into LB liquid medium, oscillation culture, centrifugation and resuspending the bacterial body with a protective agent solution; obtaining a spore suspension by inoculating Phanerochaete chrysosporium into a potato glucose agar plate and static culture; obtaining a mixed suspension by mixing the modified biochar wet material, the bacterial suspension and the spore suspension with a sodium alginate solution, and obtaining gel microspheres by dropping the mixed suspension into a calcium chloride solution and taking out and drying. The ecological activation remediation material prepared by the present application can efficiently fix heavy metals, enhance the survival time and long-acting effect of microorganisms in the soil, and promote the recovery of the ecological function of the soil.

The invention relates to the technical field of high polymer materials, and discloses a flame-retardant polyolefin cable material and a preparation method thereof.The preparation method of the flame-retardant polyolefin cable material comprises the following steps that UPy-NCO reacts with hydroxyethyl methylacrylate, and UPy containing carbon-carbon double bonds is obtained; carrying out copolymerization on UPy containing carbon-carbon double bonds, double-bond-terminated polysiloxane and glycidyl methacrylate to obtain a polymer modifier; preparing BN (at) ZIF-67 (at) PDA (potato dextrose agar); the BN (at) ZIF-67 (at) PDA is subjected to modification treatment through a silane coupling agent KH-550 and a polymer modifier in sequence, and the multifunctional flame retardant is obtained; and weighing the polypropylene resin, the functionalized polypropylene, the multifunctional flame retardant and the auxiliary agent in proportion, mixing, extruding and granulating to obtain the flame-retardant polyolefin cable material. The flame-retardant polyolefin cable material disclosed by the invention is good in flame retardant property, excellent in mechanical property, and excellent in water resistance, heat resistance, heat-conducting property and insulating property.

The application belongs to the technical field of composite materials, and specifically provides a sulfur-doped mycelium bamboo-based composite material and a preparation method thereof. The application prepares a potato glucose agar culture medium first, then carries out the propagation of white rot fungi based on the potato glucose agar culture medium, then cultures the bamboo powder carbon powder prepared under specific conditions and the white rot fungi in a liquid culture medium to obtain white rot fungi bamboo powder spheres, finally carries out a dipping reaction of the obtained white rot fungi bamboo powder spheres and sodium thiosulfate, collects the solid material after the reaction is completed, and dries the solid material, finally carries out a heating treatment under a nitrogen atmosphere, and finally the sulfur-doped mycelium bamboo-based composite material is prepared, which has high adsorption of heavy metal ions and can effectively remove heavy metal ions when used for treating mercury-containing wastewater.

The invention designs a preparation method of an active medicinal and edible composite nutrient, and the method comprises the following steps: carrying out initial culture on a ganoderma lucidum monoclonal isolate through a potato dextrose agar plate, and then transferring the ganoderma lucidum monoclonal isolate to a specific liquid culture medium for shake culture; after detecting that the activity of beta-glucosidase reaches 100 mU / mL, centrifuging to take supernatant, and adding rhizoma polygonati, lucid ganoderma and mulberry leaf powder for biotransformation; and inoculating lactobacillus plantarum, adding a freeze-drying protective additive, freeze-drying, and sieving to obtain the active medicinal and edible composite nutrient. According to the method, macromolecular active components of lucid ganoderma, rhizoma polygonati and folium mori are decomposed into small molecules through biotransformation, the problem that a traditional material is poor in intestinal absorption is solved, the prepared active medicinal and edible composite nutrient can restore the number of neutrophils, macrophages and T cells, meanwhile, tnf-alpha, il-12a and ifn-gamma proinflammatory gene expression is up-regulated, and the effect of improving the intestinal absorption rate is achieved. In addition, the natural source is high in safety, and can be used for an immune function regulation scene.

The invention discloses a method for efficiently screening tomato fusarium wilt resistant varieties. Relates to the technical field of vegetable disease-resistant breeding. Comprising the following steps: a, preparing a culture medium; b, sterilizing the culture medium and test equipment; c, disinfecting the tomato seeds; d, placing the disinfected tomato seeds on a culture medium, and culturing; e, activating tomato fusarium oxysporum on a potato dextrose agar culture medium; f, inoculating fusarium oxysporum; and g, after co-culture, identifying the fusarium wilt resistant tomato rootstock germplasm material by investigating disease indexes. The method can replace a tomato variety living body screening test, and screening of tomato fusarium wilt resistant germplasm materials can be completed in the tissue culture process (bottle). The screening time of the tomato fusarium wilt-resistant rootstock is greatly shortened, the test intensity of scientific researchers is reduced, meanwhile, the interference of an external bad environment is overcome, and the accuracy of screening the tomato fusarium wilt-resistant rootstock is improved.

The invention relates to a cordyceps sinensis cephalosporium liquid fermentation process. The process comprises the following steps: strain purification: (1) culturing cordyceps sinensis cephalosporium through a PDA (potato dextrose agar) culture medium; (2) screening out pure white hyphae from the hyphae cultured in the step (1), and culturing the pure white hyphae in a slant enriched culture medium; preparing multi-stage liquid seeds; 1, flow optimization: a four-stage fermentation process shortens the production period and improves the productivity; 2, cost advantage: the culture medium formula is simplified, and the raw material utilization rate is improved; 3, the quality is stable, the temperature is accurately controlled, and the batch consistency is ensured; and 4, environmental protection and energy conservation: energy consumption is reduced, and green production requirements are met. And 5, a breeding tank is newly added, a four-stage fermentation system is formed, and the strain amplification efficiency is improved. And 6, the culture medium formula is optimized, and the mycelium biomass and the product concentration are remarkably improved.

The invention discloses a preparation method of stropharia rugoso-annulata cultivated species. The preparation method comprises the following steps: selecting pollution-free stropharia rugoso-annulata slant mother species with white and robust hyphae and a PDA (potato dextrose agar) substrate; preparing a shake flask liquid culture medium, sterilizing and inoculating; carrying out shake-flask culture and hypha scattering; preparing a cultivation bag and sterilizing; and carrying out sterile inoculation and spawn running culture and the like. According to the method, by optimizing a culture medium formula, an inoculation process and culture conditions, the seed production time of the stropharia rugoso-annulata is effectively shortened, efficient preparation is achieved, the quality of the obtained cultivated seeds is good, and the efficient and high-quality requirements of modern agriculture are met.

The invention discloses a device and method for producing dextranase through chaetomium gracile fermentation, and relates to the technical field of dextranase production.The device comprises a tank body and further comprises an oscillation stirring part, the oscillation stirring part comprises a rotating shaft which rotates and slides up and down on the tank body, and the rotating shaft is connected with the oscillation stirring part; a stirrer is fixedly mounted at one end, positioned in the tank body, of the rotating shaft; comprising the following steps: S1, taking out a chaetomium graciliforme strain from a glycerol tube at-80 DEG C, inoculating the chaetomium graciliforme strain to a potato dextrose agar slant culture medium in an ultra-clean workbench, and culturing at 28 DEG C for 5 days until a bacterial colony is in an off-white villus shape. The device has the advantages that the growth of thalli and the synthesis of dextranase can be effectively improved through up-and-down reciprocating oscillation, a fermentation sample can be automatically and quantitatively extracted at regular time in the up-and-down reciprocating oscillation process, manual sampling is not needed, improper influence on subsequent fermentation degree judgment caused by misoperation can be avoided, and the working efficiency is improved. And a worker can more accurately analyze and judge the fermentation progress.