26 results about "Potato dextrose agar" patented technology

The invention discloses a high-performance modified resin material and a preparation method thereof, and belongs to the technical field of resin composite material preparation. The high-performance modified resin material is prepared from a main agent, a curing agent and nano filler, the main agent is prepared from 70 to 100 parts of bisphenol epoxy resin, 20 to 40 parts of dopamine modified epoxy resin, 5 to 15 parts of diglycidyl ether, 3 to 15 parts of flame retardant, 0.3 to 1 part of anti-settling agent, 0.05 to 0.3 part of defoaming agent and 0.1 to 0.5 part of pigment; the curing agent is prepared from the following components in parts by weight: 15-35 parts of a rapid curing agent, 10-30 parts of a toughening curing agent, 5-15 parts of a dopamine modified amine curing agent, 0.5-3 parts of an accelerant and 0.1-0.5 part of an antioxidant; and the nano filler is nano-particle coated PDA (potato dextrose agar). Amino of dopamine reacts with epoxy group, catechol is oxidized to generate a quinoneform structure, thiol-epoxy click reaction is catalyzed, and the material can be rapidly cured at low temperature; a flexible chain (-S-CH-CH-) of polymercaptan and a polyether chain of amine-terminated polyether are synergistically toughened, so that the toughness and impact resistance of the material can be improved.

The invention discloses an auricularia plicata strain and a cultivation and domestication method thereof, and particularly relates to an auricularia plicata strain W-24 (Jiangxi No.1) and a cultivation and domestication method thereof, the auricularia plicata strain W-24 is preserved in China General Microbiological Culture Collection Center on June 11, 2024; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The cultivation method comprises the following steps: firstly, activating auricularia plicata strains on a PDA (potato dextrose agar) comprehensive culture medium, inoculating the auricularia plicata strains into a solid culture medium, and culturing at 24-26 DEG C for 30-35 days to obtain solid stock seeds; the solid stock seeds are inoculated into cultivation bags, the inoculation amount is 10-15 g / bag, the cultivation bags are sealed, the cultivation bags are placed in a cultivation room to be cultivated in a dark place at the constant temperature of 24-26 DEG C, after cultivation is carried out for 80-85 days, hyphae grow all over the fungus bags, and fruiting management is carried out; the auricularia plicata strain W-24 provided by the invention has the characteristics of short cultivation period, high yield, strong stress resistance and the like, and has a wide market prospect.

The invention belongs to the field of biology, and particularly relates to a separation method of achnatherum inebrians # imgabs0 # endophytic fungi. The invention firstly provides a biological culture medium for separation of achnatherum inebrians # imgabs1 # endophytic fungi, wherein the biological culture medium is a potato dextrose agar culture medium containing antibiotics, yeast extract and achnatherum inebrians leaf leachate. Secondly, the invention provides a separation method of the achnatherum inebrians # imgabs2 # endophytic fungi, and the method comprises the following steps: disinfecting achnatherum inebrians seeds, culturing the achnatherum inebrians seeds in the culture medium for 10-14 days, and purifying unpolluted hyphae at the edge of a bacterial colony to obtain the achnatherum inebrians # imgabs3 # endophytic fungi. Compared with a traditional method, the method has the advantages that the separation rate of the endophytic fungi is increased from 5% to 18%, the pollution rate is reduced from 17% to 6%, and the growth speed of the endophytic fungi is increased from 2.6 mm / day to 3.1 mm / day.

The invention relates to the technical field of microbial genetic transformation, and particularly discloses a PEG (Polyethylene Glycol)-mediated camellia anthracnose protoplast genetic transformation method which comprises the following steps: obtaining young and tender camellia anthracnose mycelia, filtering and collecting protoplast by using double-layer lens wiping paper, obtaining protoplast precipitate, purifying the protoplast by using an STC solution, and resuspending the protoplast by using the STC to obtain the PEG-mediated camellia anthracnose protoplast. The preparation method comprises the following steps: adding plasmids into a camellia anthracnose protoplast, lightly and uniformly mixing, standing at room temperature, adding a PTC solution, uniformly mixing, standing at room temperature, adding a PTC solution, uniformly mixing, standing at room temperature, pouring into a TB3 solid culture medium, uniformly mixing, pouring into a plate, culturing overnight, covering with a potato dextrose agar culture medium containing geneticin, and culturing at room temperature. The transformant is inoculated to a potato dextrose agar culture medium containing heredity mycin, the transformant with stable characters is screened, and the strain obtained through genetic transformation is obtained.By means of the method, construction of genetic transformation of the camellia anthracnose bacteria is achieved, the time needed by genetic transformation is shortened, and the transformant expressing red fluorescence and stably inheriting is obtained.

The invention relates to the technical field of food fermentation, in particular to a multi-stage strain fermentation and sterilization packaging method of red yeast rice. The method comprises the following steps that a high-quality monascus strain is selected and inoculated to a solid culture medium, the solid culture medium is prepared from 200 g of potatoes, 20 g of glucose, 15-20 g of agar and 1000 mL of tap water, and the pH value of the solid culture medium is 5.0-6.0; culturing in a constant-temperature incubator at 28-32 DEG C for 5-7 days to obtain a primary strain; transferring the primary strain to a liquid culture medium, wherein the liquid culture medium comprises the following components: 130 g of malt paste powder, 0.1 g of chloramphenicol and 1000 mL of water; and culturing in a constant-temperature shaking table at 28-30 DEG C at the speed of 150-200 revolutions per minute for 3-5 days to obtain a secondary strain. According to the multi-stage strain fermentation and sterilization packaging method for the red yeast rice, provided by the invention, the traditional method is low in strain activity and weak in reproductive capacity and directly restricts the fermentation efficiency due to extensive strain culture, and a culture medium formula and culture parameters are customized for strains in different stages through three-stage strain staged culture.

The invention discloses a method for producing a butanol system through fungus-bacterium co-culture fermentation alkali pretreatment of rice straw, and belongs to the technical field of microbial fermentation production of clean energy. The problem that a large number of cellulase preparations need to be used in the butanol fermentation process of existing lignocellulose biomass is solved. The method comprises the following steps: 1, pre-culturing clostridium beijerinckii in a reinforced clostridium culture medium; 2, culturing trichoderma reesei in potato dextrose agar, collecting spores, and inoculating the spores into a spore culture medium for culturing; and 3, inoculating the trichoderma reesei inoculum into a co-culture medium taking straws as a carbon source for culture, and then inoculating the clostridium beijerinckii inoculum. The method is used for a system for producing butanol by pretreating rice straws through fungus-bacterium co-culture fermentation alkali.

The invention discloses a preparation method of a BN-doped photo-thermal super-hydrophobic coating on the surface of an aluminum alloy. The preparation method is specifically implemented according to the following steps: step 1, preparing mesoporous ferroferric oxide nanoparticles; step 2, coating the mesoporous ferroferric oxide nano particles with titanium dioxide; step 3, functionalizing the boron nitride nanosheet by using polydopamine; step 4, synthesizing TiO2 (at) Fe3O4 / BNNSs-PDA (Potato Dextrose Agar); and 5, constructing the photo-thermal self-repairing super-hydrophobic coating. The invention also discloses the coating prepared by the method. According to the invention, the BNNSs are subjected to polydopamine modification, and the nano-scale modified TiO2 coated Fe3O4 is attached to the surface by using polyfunctional groups of polydopamine, so that the prepared composite material has excellent photo-thermal conversion performance and also provides lasting super-hydrophobic performance.

The present application belongs to the technical field of contaminated soil remediation, and relates to an ecological activation remediation material for heavy metal contaminated soil and a preparation method thereof, comprising: obtaining biochar by anaerobic pyrolysis, crushing and sieving of corn stalks; obtaining modified biochar wet material by dispersing the biochar in a modified solution, mixing, stirring and heating; obtaining a bacterial suspension by inoculating Burkholderia into LB liquid medium, oscillation culture, centrifugation and resuspending the bacterial body with a protective agent solution; obtaining a spore suspension by inoculating Phanerochaete chrysosporium into a potato glucose agar plate and static culture; obtaining a mixed suspension by mixing the modified biochar wet material, the bacterial suspension and the spore suspension with a sodium alginate solution, and obtaining gel microspheres by dropping the mixed suspension into a calcium chloride solution and taking out and drying. The ecological activation remediation material prepared by the present application can efficiently fix heavy metals, enhance the survival time and long-acting effect of microorganisms in the soil, and promote the recovery of the ecological function of the soil.

The invention relates to the technical field of high polymer materials, and discloses a flame-retardant polyolefin cable material and a preparation method thereof.The preparation method of the flame-retardant polyolefin cable material comprises the following steps that UPy-NCO reacts with hydroxyethyl methylacrylate, and UPy containing carbon-carbon double bonds is obtained; carrying out copolymerization on UPy containing carbon-carbon double bonds, double-bond-terminated polysiloxane and glycidyl methacrylate to obtain a polymer modifier; preparing BN (at) ZIF-67 (at) PDA (potato dextrose agar); the BN (at) ZIF-67 (at) PDA is subjected to modification treatment through a silane coupling agent KH-550 and a polymer modifier in sequence, and the multifunctional flame retardant is obtained; and weighing the polypropylene resin, the functionalized polypropylene, the multifunctional flame retardant and the auxiliary agent in proportion, mixing, extruding and granulating to obtain the flame-retardant polyolefin cable material. The flame-retardant polyolefin cable material disclosed by the invention is good in flame retardant property, excellent in mechanical property, and excellent in water resistance, heat resistance, heat-conducting property and insulating property.

The application belongs to the technical field of composite materials, and specifically provides a sulfur-doped mycelium bamboo-based composite material and a preparation method thereof. The application prepares a potato glucose agar culture medium first, then carries out the propagation of white rot fungi based on the potato glucose agar culture medium, then cultures the bamboo powder carbon powder prepared under specific conditions and the white rot fungi in a liquid culture medium to obtain white rot fungi bamboo powder spheres, finally carries out a dipping reaction of the obtained white rot fungi bamboo powder spheres and sodium thiosulfate, collects the solid material after the reaction is completed, and dries the solid material, finally carries out a heating treatment under a nitrogen atmosphere, and finally the sulfur-doped mycelium bamboo-based composite material is prepared, which has high adsorption of heavy metal ions and can effectively remove heavy metal ions when used for treating mercury-containing wastewater.

The present invention discloses a composition for controlling rice root-knot nematodes during the rice seedling stage, as well as its preparation method and application, and belongs to the field of agricultural technology. The composition of the present invention comprises a hemp fiber film adsorbed with fluopyram for wrapping rice seedling trays, a nematode-resistant rice seedling substrate, and a rice seed coating agent; the seedling substrate comprises biochar, mineral-derived potassium humate, plant nutrient soil, perlite, vermiculite, rice straw fiber adsorbed with avermectin, amino oligosaccharide powder, and tea seed meal; and the coating agent comprises Purpurosporium lilacinum, a biomimetic component of PDA potato dextrose agar culture medium, and a viscous dispersant. The present invention also discloses a preparation method for the composition and its application for controlling rice root-knot nematodes during the rice seedling stage. Using the composition of the present invention for rice seedling cultivation results in strong soil-breaking ability of rice sprouts, high soil-breaking rate and germination rate, high seedling survival rate, uniform and good growth, and reduced root-knot nematode infestation.

The invention designs a preparation method of an active medicinal and edible composite nutrient, and the method comprises the following steps: carrying out initial culture on a ganoderma lucidum monoclonal isolate through a potato dextrose agar plate, and then transferring the ganoderma lucidum monoclonal isolate to a specific liquid culture medium for shake culture; after detecting that the activity of beta-glucosidase reaches 100 mU / mL, centrifuging to take supernatant, and adding rhizoma polygonati, lucid ganoderma and mulberry leaf powder for biotransformation; and inoculating lactobacillus plantarum, adding a freeze-drying protective additive, freeze-drying, and sieving to obtain the active medicinal and edible composite nutrient. According to the method, macromolecular active components of lucid ganoderma, rhizoma polygonati and folium mori are decomposed into small molecules through biotransformation, the problem that a traditional material is poor in intestinal absorption is solved, the prepared active medicinal and edible composite nutrient can restore the number of neutrophils, macrophages and T cells, meanwhile, tnf-alpha, il-12a and ifn-gamma proinflammatory gene expression is up-regulated, and the effect of improving the intestinal absorption rate is achieved. In addition, the natural source is high in safety, and can be used for an immune function regulation scene.

The invention discloses a method for efficiently screening tomato fusarium wilt resistant varieties. Relates to the technical field of vegetable disease-resistant breeding. Comprising the following steps: a, preparing a culture medium; b, sterilizing the culture medium and test equipment; c, disinfecting the tomato seeds; d, placing the disinfected tomato seeds on a culture medium, and culturing; e, activating tomato fusarium oxysporum on a potato dextrose agar culture medium; f, inoculating fusarium oxysporum; and g, after co-culture, identifying the fusarium wilt resistant tomato rootstock germplasm material by investigating disease indexes. The method can replace a tomato variety living body screening test, and screening of tomato fusarium wilt resistant germplasm materials can be completed in the tissue culture process (bottle). The screening time of the tomato fusarium wilt-resistant rootstock is greatly shortened, the test intensity of scientific researchers is reduced, meanwhile, the interference of an external bad environment is overcome, and the accuracy of screening the tomato fusarium wilt-resistant rootstock is improved.

The invention relates to the technical field of fungus inhibition, and discloses a method for inhibiting aspergillus flavus by using methylmalonic acid. The method for inhibiting aspergillus flavus by using methylmalonic acid comprises the following steps: S1, activating an aspergillus flavus strain; s2, preparing a culture medium for culturing aspergillus flavus, wherein the culture medium comprises a potato dextrose agar culture medium, namely a PDA culture medium, a potato dextrose broth culture medium, namely a PDB culture medium, a 1640 culture medium, a minimum salt culture medium and a Shaw liquid culture medium; s3, inoculating bacillus amyloliquefaciens and aspergillus flavus spores into the PDA culture medium, and exploring the inhibition effect of the bacillus amyloliquefaciens on the aspergillus flavus; and S4, inoculating aspergillus flavus by using a 1640 culture medium. According to the method for inhibiting the aspergillus flavus by using the methylmalonic acid provided by the invention, the methylmalonic acid can inhibit the growth of the aspergillus flavus, and meanwhile, the methylmalonic acid also can obviously inhibit the toxin production of the aspergillus flavus, so that a new selectivity is provided for the prevention and control of the aspergillus flavus.

The invention discloses a mother strain culture medium for enhancing vigor of a pilose antler mushroom strain. The mother strain culture medium is prepared from the following raw materials in parts by weight: 200 parts of potatoes, 20 parts of glucose, 12 parts of agar powder, 50 parts of kiwi-fruit juice and 1300 parts of water. Or the mother strain culture medium comprises the following raw materials in parts by weight: 150-250g of potatoes, 15-25g of glucose, 10-15g of agar powder and 1200-1400ml of water, and the content of vitamin C in the culture medium is 0.4-0.6 mol. According to the mother strain culture medium, the strain activity of mother strains can be effectively improved, the cultivation period of the pilose antler mushrooms can be shortened in the further cultivation process, the finished product quality and yield of the pilose antler mushrooms are improved, and the good practical application and popularization value is achieved.

The invention discloses a method for maintaining the vitality of an edible mushroom strain, and relates to the technical field of edible mushroom strain preservation. The method comprises the following core steps: screening detoxified and purified strains for low-temperature domestication, and determining a low-temperature sensitive threshold based on the condition that the growth rate at low temperature is greater than or equal to 50% of the normal-temperature rate; preparing an improved culture medium which is formed by mixing wood flour, vermiculite powder and potato dextrose agar, and embedding the slow-release nutrient particles into the improved culture medium; the improved culture medium is used for short-term preservation at 4-6 DEG C, paraffin-nano coating composite protection is used for middle-term preservation, and a low-temperature dormancy awakening mechanism and nitrogen stress are combined for long-term preservation; during passage management, new hyphae with the number of splitting times smaller than or equal to 10 are selected for sterile operation; and finally, carrying out activity detection through molecular level monitoring, such as SSR (Simple Sequence Repeat) markers, and carrying out targeted improvement on strains with insufficient activity. The method can effectively maintain hypha activity, hereditary stability and fruiting performance, is standard in operation and high in applicability, and provides a reliable seed source for large-scale cultivation of shiitake mushrooms.

The invention relates to a cordyceps sinensis cephalosporium liquid fermentation process. The process comprises the following steps: strain purification: (1) culturing cordyceps sinensis cephalosporium through a PDA (potato dextrose agar) culture medium; (2) screening out pure white hyphae from the hyphae cultured in the step (1), and culturing the pure white hyphae in a slant enriched culture medium; preparing multi-stage liquid seeds; 1, flow optimization: a four-stage fermentation process shortens the production period and improves the productivity; 2, cost advantage: the culture medium formula is simplified, and the raw material utilization rate is improved; 3, the quality is stable, the temperature is accurately controlled, and the batch consistency is ensured; and 4, environmental protection and energy conservation: energy consumption is reduced, and green production requirements are met. And 5, a breeding tank is newly added, a four-stage fermentation system is formed, and the strain amplification efficiency is improved. And 6, the culture medium formula is optimized, and the mycelium biomass and the product concentration are remarkably improved.

This invention relates to the field of agricultural microbiology, and discloses a highly effective fungal agent for controlling ginger stem base rot and its preparation method. The agent includes *Trichoderma harzianum* (accession number CCTCC NO:M 20253070), which is first inoculated onto potato dextrose agar to obtain plates covered with fresh spores. Then, wheat bran and soybean meal are mixed to adjust the initial moisture content and initial pH to prepare a solid fermentation substrate. A diluted spore suspension is uniformly inoculated into the substrate for fermentation to obtain a fermentation product. The fermentation product is mixed with an extract and shaken, then filtered through sterile gauze to obtain a crude spore solution, which is finally diluted to obtain the final product. This invention achieves control by using *Trichoderma harzianum* to antagonize the pathogen and disrupt its cell structure. The use of wheat bran and soybean meal to construct a microenvironment with a high carbon-to-nitrogen ratio yields a fermentation product with high spore production. The combination of extract elution and gauze filtration to intercept residues and prepare a crude spore solution improves the purity of the fungal agent.

The invention belongs to the field of disease-resistant breeding of sweet potatoes, and particularly relates to a sweet potato pathogenic fungus fusarium oxysporum and application thereof in sweet potato breeding. The preservation number of the fusarium oxysporum is CGMCC (China General Microbiological Culture Collection Center) NO.41910, and the fusarium oxysporum is classified and named as fusarium oxysporum. After pathogenic fusarium oxysporum is separated from infected sweet potato tubers and stems, the pathogenic fungus is identified as fusarium oxysporum by utilizing morphological and molecular means, and hyphae of the fungus are pink and have large and small spores after being cultured in a PDA (potato dextrose agar) culture medium. After normal sweet potato blocks are inoculated with the fungus, the morbidity condition of the sweet potato blocks is observed, the H2O2 content, the MDA content, the proline content, the SOD enzyme activity, the soluble sugar content and the susceptible area are measured, and the disease resistance of different sweet potato varieties can be obtained through comprehensive analysis of a fuzzy membership function, so that reference is provided for disease resistance improvement of sweet potatoes and breeding of new varieties.

The invention belongs to the technical field of microbial culture media, and particularly relates to a culture medium for magnaporthe oryzae spore production, which comprises the following components in proportion: 133.33 g / L of potato, 13.33 g / L of glucose and 16.67 g / L of agar powder are contained in a solution prepared from V8 juice and distilled water according to the proportion of 1: 2; or 100 g / L of juice obtained by squeezing mature tomatoes, 20 g / L of oats, 0.3 g / L of calcium carbonate powder and 12.5 g / L of agar powder are contained in a solution prepared from the rice straw leachate and distilled water according to the ratio of 1: 1. On the premise of not increasing any acquisition difficulty and economic cost, two efficient spore production culture medium formulas and preparation methods are researched, the spore production efficiency of the two efficient spore production culture media is obviously superior to that of all common culture media in the field, and a new thought is provided for efficient culture of the magnaporthe oryzae.

The invention discloses a preparation method of stropharia rugoso-annulata cultivated species. The preparation method comprises the following steps: selecting pollution-free stropharia rugoso-annulata slant mother species with white and robust hyphae and a PDA (potato dextrose agar) substrate; preparing a shake flask liquid culture medium, sterilizing and inoculating; carrying out shake-flask culture and hypha scattering; preparing a cultivation bag and sterilizing; and carrying out sterile inoculation and spawn running culture and the like. According to the method, by optimizing a culture medium formula, an inoculation process and culture conditions, the seed production time of the stropharia rugoso-annulata is effectively shortened, efficient preparation is achieved, the quality of the obtained cultivated seeds is good, and the efficient and high-quality requirements of modern agriculture are met.

The invention discloses a device and method for producing dextranase through chaetomium gracile fermentation, and relates to the technical field of dextranase production.The device comprises a tank body and further comprises an oscillation stirring part, the oscillation stirring part comprises a rotating shaft which rotates and slides up and down on the tank body, and the rotating shaft is connected with the oscillation stirring part; a stirrer is fixedly mounted at one end, positioned in the tank body, of the rotating shaft; comprising the following steps: S1, taking out a chaetomium graciliforme strain from a glycerol tube at-80 DEG C, inoculating the chaetomium graciliforme strain to a potato dextrose agar slant culture medium in an ultra-clean workbench, and culturing at 28 DEG C for 5 days until a bacterial colony is in an off-white villus shape. The device has the advantages that the growth of thalli and the synthesis of dextranase can be effectively improved through up-and-down reciprocating oscillation, a fermentation sample can be automatically and quantitatively extracted at regular time in the up-and-down reciprocating oscillation process, manual sampling is not needed, improper influence on subsequent fermentation degree judgment caused by misoperation can be avoided, and the working efficiency is improved. And a worker can more accurately analyze and judge the fermentation progress.

The invention discloses a fungus which is screened from tremella fuciformis seeds and has the capability of degrading Shanxi lignite (hereinafter referred to as lignite), the number of the fungus is A-1, the fungus is identified as Trichoderma citrinoviride through 26S r DNA D1 / D2 region sequencing, belongs to Trichoderma spp, is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC 23821, and the preservation date is November 23, 2021. According to the invention, the strain A-1 is primarily screened from tremella seeds, the strain A-1 is obtained from an improved potato dextrose agar culture medium taking coal as an added carbon source, and the degradation process conditions of the strain on Shanxi lignite are optimized by taking the degradation rate as an index: the environment temperature is 28.4 DEG C, the environment pH value is 6.48, the inoculation amount is 3mL, the coal sample granularity is 120-150 meshes, the coal slurry concentration is 1.0125 g.50 mL <-1 >, and the degradation time is 24 days. At the moment, the actual degradation rate of the trichoderma citrinoviride for degrading the Shanxi lignite is (44.34 + / -0.47)%. A foundation is provided for microbial degradation of the low-rank coal, and resource utilization of the low-rank coal is realized.

The invention discloses a method for promoting growth of carya illinoensis scab pathogen sporophore, and relates to the technical field of microbial growth promotion. The invention discloses a method for promoting the growth of sporospora fusiforme, which comprises the following steps: inoculating sporospora fusiforme cakes into a PDA (Potato Dextrose Agar) culture medium with the pH of 5-10, adding 0.2-2.5% of apocarya fruit green peel juice per 20mL, culturing in an environment at the temperature of 10-35 DEG C, and finally screening to obtain a conclusion that the most suitable growth pH of sporospora fusiforme hyphae is 6, the most suitable growth culture medium is the PDA culture medium, and the most suitable growth pH of the sporospora fusiforme hyphae is 6. The optimal growth temperature is 25 DEG C, the optimal growth carbon source is glucose, the optimal growth nitrogen source is sodium nitrate, and the optimal addition amount of the carya illinoensis green seedcase juice is 0.4% per 20 mL. The invention screens out the optimal culture medium for promoting the growth of the sporospora dispersoides, and provides a basis and technical support for the follow-up related research of the carya illinoensis scab.

The invention relates to the technical field of phytopathology, in particular to a method for artificially inducing asarum leaf blight germs to massively produce spores, which comprises the following steps: S1, strain activation: inoculating the asarum leaf blight germs into a PDA (potato dextrose agar) culture medium, and carrying out illumination culture at 20 DEG C for 7 days; s2, spore production induction: inoculating the activated asarum leaf blight germs into an optimized spore production culture medium, and culturing for 14 days at the temperature of 20 DEG C and the initial pH value of 7 by adopting near ultraviolet light illumination to obtain a large number of spores; the optimized spore production culture medium comprises the following components: 200mL / L of V8 juice, 0.3 g / L of CaCO3, 1g / L of glucose, 1g / L of potassium nitrate and 15-20g / L of agar. The invention establishes a rapid, efficient, simple and convenient asarum in-vitro spore production method, and the method can effectively solve the technical problem that spores are difficult to produce under laboratory conditions and conventional culture conditions, and provides sufficient experimental materials for deep analysis of pathogenic mechanisms of asarum.

The present invention provides an effective and safe green fungicide and its application. The green fungicide is a composition containing nicotinamide adenine dinucleotide (NAD). Nicotinamide adenine dinucleotide (NAD) treatment significantly inhibits the growth of Fusarium solani on potato dextrose agar (PDA); at the same time, after NAD treatment of bananas, the resistance of banana fruits to Fusarium solani is improved, resulting in a significant decrease in the pathogenicity of Fusarium solani to banana fruits, and the diameter of the lesions on the banana peel after NAD treatment is significantly smaller than that of the control group. Therefore, NAD treatment can be used to prepare a new antifungal preservative and is applied in the field of prevention and control of post-harvest fungal diseases of fruits.