83 results about "Mannitol solution" patented technology

The invention which belongs to the medicine processing field concretely relates to a preparation method of a long-circulating nanoparticle. The preparation method of the long-circulating nanoparticle provided in the invention has the advantages of simple operation, and good targeting and good in vitro release of products. The method comprises the following steps: 1, dissolving 5-Fu (5-fluorouracil), PEG-PHDCA (polyethylene glycol-poly(hexadecyl cyanoacrylate)) and a phosphatide in a mixed organic solvent of tetrahydrofuran and ethanol to form an organic phase; 2, slowly adding the organic phase to a water phase of a surfactant in a dropwise manner under magnetic stirring, and fully diffusing the organic phase by continuously stirring for 1h after finishing the dropwise addition; 3, carrying out reduced pressure evaporation to remove the organic solvent to obtain a nanoparticle colloidal suspension with a blue opalescence; 4, carrying out ultracentrifugation separation deposition on the nanoparticle, and washing; and 5, carrying out ultrasonic dispersion with a 4% mannitol solution, and freeze-drying.

The invention relates to the technical field of stem cells, in particular to a placenta-derived mesenchymal stem cell freezing medium. The placenta-derived mesenchymal stem cell freezing medium comprises the following components in parts by weight: 40-70 parts of DEME culture medium, 2-6 parts of sodium glycerophosphate solution, 1-5 parts of hydroxyethyl starch, 1-4 parts of mannitol solution, 0.3-0.8 part of ethanediol, 0.5-2 parts of polyvinyl alcohol, 0.5-1.8 parts of trehalose, and 0.5-1.5 parts of polyvinylpyrrolidone. With the adoption of the freezing medium for storing placenta-derived mesenchymal stem cells, the damage on the stem cells in the freezing process can be reduced, and the survival rate of the stem cells after thawing is increased.

The invention relates to the field of plant breeding and provides a pistil treatment reagent for promoting plant wide hybrid seed set and an application method thereof. The reagent is mainly composed of boric acid, CaCl2, bovine serum albumin, mannitol solution and the like. The reagent is sprayed to the pistil stigma within 10-30 minutes before pollination, contributes to improving the setting percentage and has favorable influences on the seedling survival rate. By utilizing the method provided by the invention, cross-incompatibility among plant species can be effectively overcome, and the crossing barrier between ordinary wheat and pyrethrum and plants which is in the same family, but is in different genera, is effectively overcome. The experiment proves that the setting percentage of hybrid seeds is improved due to the method, the seed plumpness is high, and a novel way is provided for obtaining superior hybrid for plant wide hybrid.

The invention relates to a conventional low-temperature preservation method of a volvariella volvacea strain. The preservation method comprises the following steps: (1) transferring the volvariella volvacea strain onto a PDA (potato dextrose agar) plate, performing static culturing in an incubator at 30-32 DEG C, then transferring the strain to a PDA test tube slant after mycelia permeate the plate, performing static culturing at 30-32 DEG C for 3-7d; (2) preparing a mannitol solution which is 5-20% in mass fraction, and then filtering and sterilizing; and (3) transferring the mannitol solution in the step (2) to the test tube slant which is full of the mycelia in the step (1) by virtue of a sterile injector, and preserving the test tube slant at conventional low temperature. The preservation method disclosed by the invention can prolong a preservation duration of the volvariella volvacea mycelia in a low-temperature environment of 3-6 DEG C; the preservation method has the advantages of simple and convenient operation, low cost, long preservation duration of the strain, rapid recovery and growth, good stability and the like; and the preservation method is especially suitable for small and medium-sized enterprises and farmers planting the volvariella volvacea.

The present invention relates to a lyophilized preparation of a cytochrome C-containing pharmaceutical composition for injection, and the lyophilized preparation comprises the following components by weight: 75-300 parts of cytochrome C; 30-60 parts of L-arginine; 2-5 parts of disodium edetate; and 150 parts of mannitol. In the lyophilized preparation, disodium edetate and L-arginine are selectd as excipients, thereby helping to improve the stability of cytochrome C; in the preparation process of the liquid pharmaceutical composition, mannitol is used as a lyophilizing excipient, and phosphoric acid or a sodium phosphate solution is used as an pH adjusting agent, thereby enabling the product quality more stable; and after a mannitol solution is treated activated carbon, the cytochrome C is added, thereby preventing the problem of decrease in the cytochrome C level due to the adsorption by activated carbon. The preparation method of the present invention shortens the lyophilized time from 23 hours to 14 to 16 hours, and reduces energy consumption by 30%; while the production capacity is improved by about 30% without increasing hardware input, thereby significantly reducing the cost of production.

The invention discloses a preparation method of a mannitol-silica core-shell structured PCM (phase-change material) microcapsule. The method comprises the following steps: preparing a mannitol solution serving as a dispersed phase and preparing an organic solution serving as a continuous phase to prepare a stable water-in-oil emulsion system; adding two kinds of silicon source precursors into the continuous phase in certain proportion so that the precursors are subjected to hydrolytic polycondensation at an oil-water interface to form a thickness-controllable silica capsule wall, thereby realizing the coating to the mannitol aqueous solution; and carrying out water removal so as to obtain a silica coated mannitol PCM microcapsule. The method disclosed by the invention firstly solves the microencapsulation problem of phase-change materials (used at a temperature of above 80 DEG C) under an approximate room temperature condition, and secondly solves the technical problems of the preparation of thick-walled silica microspheres. Through an appropriate raw material ratio and an appropriate process, a PCM microcapsule which has stable and good coating capacity is prepared, and the prepared PCM microcapsule can be applied to the fields of high-temperature thermal storage, protection and the like and has a high application potential.

The invention aims at providing a lansoprazole lyophilized medicine composition used for injection. According to the invention, lansoprazole is dissolved in a solution of sodium hydroxide and mannitol; the obtained solution is lyophilized, such that the medicine composition is obtained. In the medicine composition, a weight ratio of lansoprazole:mannitol:sodium hydroxide is 2-8.5:2-8.5:1, and preferably 6:6:1. The medicine composition is advantaged in simple formula and small amount of auxiliary materials. Therefore, side effects caused by excess dosages of auxiliary materials are overcome; a lyophilization time is greatly reduced, such that unnecessary energy consumption is saved. Therefore, the lansoprazole medicine composition has certain competitive strength in markets.

The invention relates to a preparation method of long-circulating nanoparticles and belongs to the field of drug processing. The preparation method can be operated simply and can produce the long-circulating nanoparticles having good targeting performances and external release properties. The preparation method comprises the following steps of dissolving 5-Fu, PEG-PHDCA and phospholipid in a mixed organic solvent of tetrahydrofuran and ethanol to obtain an organic phase, slowly and dropwisely adding the organic phase into a water phase of a surfactant with magnetic stirring, then persistently stirring for 1 hour so that the organic phase is dispersed fully, carrying out reduced pressure evaporation to remove the mixed organic solvent and to obtain a nanoparticle colloidal suspension liquid having blue opalescence, carrying out overspeed centrifugal separation precipitation and washing of nanoparticles, carrying out ultrasonic dispersion by a mannitol solution having the content of 4%, and carrying out freeze drying.

The invention relates to a method for rapidly measuring trace boron impurities in polysilicon. The method comprises the following steps: weighting a polysilicon sample; adding a mannitol solution into the sample; adding a potassium carbonate solution into the sample; adding hydrofluoric acid, then slowly dropping nitric acid in the sample and carrying out acidified dissolving; after the sample is dissolved, heating the obtained object, and stopping heating until white smoke is emitted completely; adding hydrochloric acid into the sample and soaking, diluting with a small amount of pure water, and cooling; adding methanol into the solution, uniformly shaking and making up to volume; rinsing ICP-MS (Inductively Coupled Plasma Mass Spectrometry) respectively by using ammonia water and pure water; and after the ICP-MS is rinsed completely, detecting the solution sample with metered volume by using ICP-MS, so that the boron content of the sample can be accurately measured. The method disclosed by the invention is simple in process, low in cost, can measure accurately, and rapidly measure the boron content of polysilicon.

The invention relates to mesoporous trimanganese tetraoxide and a preparation method thereof. The preparation method includes: mixing a potassium permanganate solution in a certain molar concentration ratio with a mannitol solution, stirring until purple fades away, adding a reducing agent, stirring for reaction, filtering, washing and drying to obtain the mesoporous trimanganese tetraoxide. The mesoporous trimanganese tetraoxide which is similar to sponge morphologically and structurally has advantages of large specific surface area, narrow pore size distribution and the like and is applicable to fields of supercapacitors, batteries, catalysis and sewage treatment. The mesoporous trimanganese tetraoxide has excellent comprehensive electrochemical performances when serving as a super electrode material, wherein a specific capacity in an aqueous electrolyte is 190-250F/g, excellent rate performance is achieved (the retention rate being 60-70% at a charging-discharging rate of 50mv/s), and high cycle stability is realized (the retention rate being higher than 99% in charging and discharging for 2000 times at 2A/g). In addition, compared with a traditional preparation method, the method has advantages of easiness in raw material acquisition, high yield, short production cycle, low equipment requirement and the like, thereby being easy for industrialization.

The invention relates to a pretreatment method for polysilicon impurity detection, and particularly relates to a polysilicon pretreatment method regulating dropwise addition concentration of nitric acid. The method comprises the following steps: (1) weighing 0.1-0.5g of polysilicon sample in a PTFE (polytetrafluoroethylene) beaker; (2) adding 0.3-1ml of 1% mannitol solution into the PTFE beaker to drench the polysilicon sample; (3) adding 3-5ml of hydrofluoric acid having a mass percent of 49% into the PTFE beaker; (4) adding 0.5-1.0ml of nitric acid having a mass percent of 35%, then adding 1.0-2.0ml of nitric acid having a mass percent of 70%, and reacting until the polysilicon sample is completely dissolved; and (5) controlling the temperature to volatilize silicon, diluting to a specified volume, regulating the acidity, detecting the impurity content, and ending the operation. The polysilicon pretreatment method provided by the invention has the following advantages: element boron and other metal elements in the polysilicon can be simultaneously detected; meanwhile, the element boron reservation effect is remarkable, and the recovery rate can be increased from 80-85% to 95% or above; the method is simple and easy to operate; the evaporation drying time of the solution is shortened; and the polysilicon pretreatment efficiency is enhanced.

The invention discloses a preparation method of a t-Se rod supported BiOCl ultrathin nanosheet composite photocatalyst, and belongs to the technical field of environmental material preparation. The method comprises the following specific steps of: adding cadmium chloride and methacrylic acid into deionized water serving as a solvent, adjusting the pH value of the solution after stirring, injectingsupernatant liquid obtained after dissolving selenium powder and sodium borohydride in deionized water, introducing nitrogen, pouring the solution into a three-neck flask for heating, naturally cooling the solution, and carrying out centrifuging, washing and drying to obtain CdSe quantum dots; and mixing a BiOCl precursor solution and the CdSe quantum dots in a mannitol solution with stirring, pouring the mixture into a reaction kettle, naturally cooling the mixture to room temperature after hydrothermal reaction for a certain period of time, opening the kettle, carrying out centrifugal washing and drying, and carrying out drying to obtain a composite photocatalyst. The composite photocatalyst provided by the invention can effectively utilize visible light to degrade tetracycline hydrochloride in antibiotic wastewater, is simple and convenient to operate, and is a green and environment-friendly high-efficiency treatment technology.

The invention provides a method for quickly separating germ cells of angiosperms. The pollen of the angiosperms is put in mannitol solutions for a period of time for releasing to obtain the germ cells. The method for separating the germ cells of the angiosperms is short in separation time and simple in steps, plenty of active germ cells of the angiosperms can be obtained, germ cell membranes are complete, and the method has a large practical application value.

The invention relates to an ultrasound-sensitive liposome and application thereof, and aims to effectively solve the problems of large toxic and side effects, poor curative effect, low treatment efficiency and high immune toxicity in chemotherapy. According to the technical scheme, the ultrasound-sensitive liposome is prepared by the following steps: weighing 2 to 10mg of cholesterol, 2 to 20mg of 1,2-dipalmitoyl phosphatidylglycerole (DPPG), 5 to 30mg of dipalmitoyl phosphatidylcholine (DPPC) and 0 to 20mg of dioleoyl phosphatidyl ethanolamine (DOPE); dissolving the weighed materials in 5 to 30m of solvent; performing ultrasonic treatment for 5 to 60 minutes till the materials are fully dissolved; performing rotary evaporation at the temperature of 50 DEG C to remove the solvent; performing rotary evaporation under the vacuum condition for 4 hours to remove residual solvent; adding 1 to 10mg of medicinal mannitol containing solution, wherein the mannitol concentration is 0.2 to 1M; quickly blowing the bottom of a bottle with hot air to accelerate hydration; performing ultrasonic treatment for 5 to 10 minutes till the solution becomes uniform and transparent; freezing and unfreezing for 3 to 6 times to obtain the ultrasound-sensitive liposome. Medicaments wrapped in the liposome are released at a fixed point, so that the toxic and side effects of chemical medicaments are lowered, and the curative effect is enhanced; the ultrasound-sensitive liposome can be used as an ultrasonic imaging diagnostic agent, and is an innovation on chemotherapeutic medicaments.

The invention belongs to the technical field of agriculture planting and discloses a planting method capable of reducing content of cyanophoric glycosides in cherry fruits. The method comprises steps as follows: (1) forest land control; (2) forest land clearing; (3) density control; (4) preparation of afforestation land; (5) application of base fertilizer and backfilling of surface soil: 10-20 kg of compost is applied to each hole while backfilling of the surface soil is performed, and 5-10 kg of a mannitol solution is sprayed to each hole; (6) selection of seedlings; (7) planting; (8) cultivation management. The planting method capable of reducing the content of cyanophoric glycosides in the cherry fruits is provided, the content of cyanophoric glycosides in the cherry fruits is reduced, and the economic benefits of planting are improved.

The invention relates to the technical field of chemical extraction of effective components, in particular to a process for extracting polysaccharide from hericium erinaceus spore powder, and the process comprises the following steps: S1, carrying out wall breaking treatment on the hericium erinaceus spore powder to obtain wall-broken hericium erinaceus spore powder; s2, mixing the wall-broken hericium erinaceus spore powder with a mannitol solution according to a solid-to-liquid ratio of 1g: (60-88) mL, and carrying out heating treatment for 45-70 minutes to obtain a wall-broken hericium erinaceus spore powder solution; s3, introducing nitrogen into the wall-broken hericium erinaceus spore powder solution, keeping for 12 minutes to half an hour under the pressure condition of 1-1.3 MPa, and quickly reducing the pressure to standard atmospheric pressure to obtain a polysaccharide crude extract; and S4, carrying out alcohol precipitation separation on the polysaccharide extracting solution, concentrating and drying to obtain the polysaccharide extract. The process provided by the invention is high in polysaccharide extraction yield, high in weight-average molecular weight of polysaccharide, low in equipment requirement and suitable for large-scale industrial production.

The invention relates to the field of a pharmaceutical preparation, and particularly relates to a sodium glycididazole composition and a preparation method thereof. The sodium glycididazole composition includes sodium glycididazole, mannitol, and sodium bicarbonate. The preparation method comprises the steps of dissolving mannitol by injection water under room temperature to obtain mannitol solution; slowly adding the sodium glycididazole material to the mannitol solution, slowly agitating and dissolving to obtain soup, dissolving the sodium bicarbonate by the injection water to obtain the sodium bicarbonate solution; adjusting the pH of the soup to 6.8-7.8 by the sodium bicarbonate solution, supplying the injection water, filtering, bulking, freeze-drying and capping. The sodium glycididazole composition not only is simple in prescription and process, but also is stable in preparation, reduces the cost, is suitable for industrial production, greatly avoids and reduces the loss of the sodium glycididazole, and ensures development of the pesticide effect.

The invention relates to a fresh-keeping method of straw mushrooms. The method comprises the steps that 1, a prepared straw mushroom cultivation material is moved into a mushroom house and subjected to pasteuring; 2, the straw mushrooms are transplanted to the cultivation material and cultivated for 5-8 days under the dark condition, and a mannitol solution of 5%-25% serves as fruiting water to be sprayed on the material surface; 3, after the straw mushrooms grow to the egg-shaped period, harvesting is conducted, and the harvested straw mushrooms are stored at 3-6 DEG C. The fresh-keeping method of the straw mushrooms can significantly improve the fresh-keeping effect of the straw mushrooms, has the advantages of being easy and convenient to operate, low in cost and the like and is suitable for production and storage of the straw mushrooms, and practical application and popularization are convenient.

The invention discloses a preparation method of physcomitrella patens protoplast. The preparation method comprises the following steps of S1, taking physcomitrella patens cormus or protonema, performing homogenization to obtain homogenate, and putting the homogenate into a BCDAT culture medium for alternate culture of 16-hour illumination and 8-hour dark treatment; S2, dissolving a mixed enzyme ina 6-10% mannitol solution, carrying out uniform vortex mixing, performing centrifuging, taking supernatant, and performing filtering to obtain a mixed enzyme solution; and S3, putting the protonema obtained in the step S1 into the mixed enzyme solution prepared in the step S2, performing uniform mixing, carrying out enzymolysis, performing filtering, performing centrifuging to remove supernatant,performing resuspending by using the 6-10% mannitol solution, and repeating the process for 2-3 times to obtain a suspension, namely the physcomitrella patens protoplast, wherein the mixed enzyme inthe step S2 is any two or three of cellulase, hemicellulase and pectinase. The protoplast prepared by the method is high in quality and good in activity, the regeneration efficiency is guaranteed on the premise that the number of protoplasts is guaranteed, and the preparation method is simple, low in cost and wide in application prospect.

The invention provides bortezomib lyophilized powder for injection. The bortezomib lyophilized powder for injection comprises the following raw materials in parts by weight: 0.1 to 0.3 part of bortezomib, 3 to 10 parts of acetonitrile, 1 to 3 parts of mannitol and 87 to 94 parts of water. A preparation process comprises the following steps: fully dissolving the raw material medicines in an acetonitrile-aqueous solution, and then putting the mixed solution into the auxiliary material, namely a mannitol solution; the bortezomib is in sufficient contact with the mannitol solution in a dissolved state to rapidly form boric acid ester, so that the problem of poor solubility of the bortezomib raw material is solved under the premise of small use amount of an organic solvent, and the stability ofan intermediate product and a preparation is improved.

The invention discloses a method for preparing octreotide acetate microsphere preparation. The method comprises the following steps: (1) dissolving octreotide acetate in methanol to form a solution A;(2) dissolving PLGA in dichloromethane to form a solution B; (3) mixing the solution A and the solution B while stirring to form a uniform solution C; (4) adding silicone oil into the solution C at aconstant speed while stirring to form a condensation product; (5) adding the obtained condensation product into curing liquid to form microspheres; (6) filtering the microspheres obtained in step (5), and cleaning; adding a proper amount of solution of mannitol, and performing vacuum drying and sieving. The method is easy to operate, the prepared product has high quality, the octreotide acetate microspheres can be prepared as required, and the microspheres are distributed quickly and uniformly and do not aggregate. According to the method disclosed by the invention, relatively few suspensionsteps are required for preparing the microspheres, the operation is simple, no special requirements are imposed on the preparation personnel, special training is not needed, and convenience in using the medicine by terminal hospitals and patients can be remarkably improved.

The invention discloses a method for acquiring a large number of sorghum protoplasts from seedlings of sorghum BTx623 through an enzymolysis method and converting the sorghum protoplasts by using a polyethylene glycol mediated method. The method comprises the following specific steps: 1, selecting full sorghum seeds, disinfecting the sorghum seeds and planting the seeds in a culture medium; 2, selecting sorghum seedlings which grow well, removing leaves and roots, reserving middle leaf sheath parts, cutting the sorghum seedlings into thin strips by using a blade, pretreating the thin strips ina mannitol solution, and then adding enzymatic hydrolysate for enzymolysis; 3, after enzymolysis is finished, enriching protoplasts by using a centrifugal method, and conducting resuspending; and 4,transforming the protoplasts by using the polyethylene glycol mediated method. A stable and efficient sorghum protoplast separation and transformation system is established in the invention; and the method is rapid and efficient in action and high in transformation rate and can be used for target gene expression, protein subcellular localization, protein interaction and the like, and a powerful tool is provided for genetic improvement and gene functional group research of sorghum.