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Process for extracting polysaccharide from hericium erinaceus spore powder

An extraction process and polysaccharide extraction technology, applied in the field of chemical extraction of active ingredients, can solve problems such as untargeted utilization of Hericium erinaceus spore powder, damage to polysaccharide function, and destruction of polysaccharide structure, and are suitable for large-scale industrial production. , The effect of high weight average molecular weight and high extraction yield

Active Publication Date: 2020-01-17
福建胜美鲜生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Hericium erinaceus spore powder is the powder released when Hericium erinaceus grows and matures. It belongs to sexual spores, similar to plant pollen. Hericium erinaceus spore powder has the same nutritional content as Hericium erinaceus, and it is effective in disease prevention and treatment. It is not inferior to Hericium erinaceus fruiting bodies or Hericium erinaceus mycelium, but there is no targeted use of Hericium erinaceus spore powder, and the utilization of Hericium erinaceus spore powder is not sufficient
[0003] Hericium erinaceus spore powder contains polysaccharides that also exist in the parenchyma of Hericium erinaceus. Utilize the extraction methods of Hericium erinaceus polysaccharides in the prior art, such as using hot water extraction, acid or alkali extraction, fermentation extraction, etc., However, the extraction rate of the former is low, and it takes a long time. The latter uses chemical methods to destroy the cell structure to extract polysaccharides. While destroying the structure of the cell wall, it will also destroy the structure of the polysaccharide, resulting in damage to the function of the polysaccharide.
[0004] The applicant’s extraction of Hericium erinaceus polysaccharides in the early stage is disclosed in the Chinese invention patent with the patent publication number CN109207549A. It is used to crush Hericium erinaceus by grinding before enzymatic extraction. After fermentation, combined with Ultrasonic treatment to obtain Hericium erinaceus polysaccharides, the degree of destruction of the polysaccharides is high, and the weight average molecular weight of the polysaccharides is low, resulting in weakened efficacy of the polysaccharides

Method used

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  • Process for extracting polysaccharide from hericium erinaceus spore powder
  • Process for extracting polysaccharide from hericium erinaceus spore powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A kind of extraction process of polysaccharide in Hericium erinaceus spore powder, comprises the steps:

[0038] S1. Hericium erinaceus spore powder is subjected to jet crushing to obtain broken wall Hericium erinaceus spore powder;

[0039] S2. The broken Hericium erinaceus spore powder was mixed with a mannitol solution with a mass concentration of 28% according to the material-to-liquid ratio of 1g: 60mL, and heated to 45°C for 70 minutes to obtain a broken Hericium erinaceus spore powder solution;

[0040] S3. Introduce nitrogen gas into the solution of Hericium erinaceus spore powder, keep it for 12 minutes under the pressure of 1 MPa, and then quickly reduce the pressure to standard atmospheric pressure to obtain the crude polysaccharide extract;

[0041] S4. Slowly add 95% ethanol with a volume concentration of 1:4 to the polysaccharide crude extract described in step S3, stir evenly, and after 12 hours of static precipitation, absorb the supernatant, and concent...

Embodiment 2

[0044] A kind of extraction process of polysaccharide in Hericium erinaceus spore powder, comprises the steps:

[0045] S1. Hericium erinaceus spore powder is broken and pulverized to obtain broken wall Hericium erinaceus spore powder;

[0046] The specific operation includes the following steps:

[0047] S-11. Add the pretreatment solution to the spore powder, the amount added is 290 ± 10g spore powder per liter of the pretreatment solution, stir evenly, and keep the temperature at 42°C for 45min to obtain the pretreatment product; the pretreatment The liquid is a mixture of purified water and glycerin, wherein the volume ratio of water to glycerin is 1:3.5;

[0048] S-12. The pre-treated substance was treated at minus 25 degrees Celsius for 70-75 minutes, and then heat-shocked at a temperature of 75±2°C for 60 minutes to obtain a primary treated substance;

[0049] S-13. Dry the primary treatment at 115°C, the second temperature at 80°C, and the third temperature at 45°C, ...

Embodiment 3

[0055] A kind of extraction process of polysaccharide in Hericium erinaceus spore powder, comprises the steps:

[0056] S1. Hericium erinaceus spore powder is subjected to jet crushing to obtain broken wall Hericium erinaceus spore powder;

[0057] The specific operation includes the following steps:

[0058] S-1. Add pretreatment liquid to the spore powder, the amount added is 320g spore powder per liter of pretreatment liquid, stir evenly, and keep warm at a temperature of 30°C for more than 45min to obtain a pretreatment product; the pretreatment liquid It is a mixture of purified water and glycerin, wherein the volume ratio of water to glycerin is 1:3.8;

[0059]S-2. The pre-treated substance was treated at minus 20 degrees Celsius for 1.5 hours, then heat-shocked at a temperature of 100 degrees Celsius for 50 minutes, and repeated again to obtain the primary treated substance;

[0060] S-3. Dry the primary treatment at 120°C, the second temperature at 90°C, and the thir...

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Abstract

The invention relates to the technical field of chemical extraction of effective components, in particular to a process for extracting polysaccharide from hericium erinaceus spore powder, and the process comprises the following steps: S1, carrying out wall breaking treatment on the hericium erinaceus spore powder to obtain wall-broken hericium erinaceus spore powder; s2, mixing the wall-broken hericium erinaceus spore powder with a mannitol solution according to a solid-to-liquid ratio of 1g: (60-88) mL, and carrying out heating treatment for 45-70 minutes to obtain a wall-broken hericium erinaceus spore powder solution; s3, introducing nitrogen into the wall-broken hericium erinaceus spore powder solution, keeping for 12 minutes to half an hour under the pressure condition of 1-1.3 MPa, and quickly reducing the pressure to standard atmospheric pressure to obtain a polysaccharide crude extract; and S4, carrying out alcohol precipitation separation on the polysaccharide extracting solution, concentrating and drying to obtain the polysaccharide extract. The process provided by the invention is high in polysaccharide extraction yield, high in weight-average molecular weight of polysaccharide, low in equipment requirement and suitable for large-scale industrial production.

Description

technical field [0001] The invention relates to the technical field of chemical extraction of active ingredients, in particular to a process for extracting polysaccharides from Hericium erinaceus spore powder. Background technique [0002] Hericium erinaceus spore powder is the powder released when Hericium erinaceus grows and matures. It belongs to sexual spores, similar to plant pollen. Hericium erinaceus spore powder has the same nutritional content as Hericium erinaceus, and it is effective in disease prevention and treatment. It is not inferior to Hericium erinaceus fruiting bodies or Hericium erinaceus mycelium, but there is no targeted use of Hericium erinaceus spore powder at present, and the utilization of Hericium erinaceus spore powder is not sufficient. [0003] Hericium erinaceus spore powder contains polysaccharides that also exist in the parenchyma of Hericium erinaceus. Utilize the extraction methods of Hericium erinaceus polysaccharides in the prior art, suc...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 肖志勇方丽金郑秋芳叶艳鹏
Owner 福建胜美鲜生物科技有限公司
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