The present invention discloses an enzyme stock solution. The enzyme stock solution is prepared from the following raw materials: ancient method prepared traditional brown sugar, pineapples, papayas, red bayberries, carambola, pleurotus geesteranus, flammulina velutipes, shiitake mushrooms, pleurotus eryngii, hericium erinaceus, dried mushrooms (picked in winter), red dates, licorice, apples, strawberries, mandarin oranges, lemons, pears, longans, litchi, mangos, watermelons, tomatoes, kiwi fruits, coconuts, sugar canes, passion fruits, cucumbers, wax gourds, spinach, Chinese cabbages, celery, cauliflowers, rapes, grapes, wood ear, bitter gourds, burdocks, aloe vera, nori, oranges, mandarin oranges, bananas, broccoli, thesium chinense, carrots, tomatoes, green plums, guava, olives, wampee, Chinese wolfberry fruits, mulberries, dragon fruits, peaches, cherries, pomegranates, kiwi fruits, muskmelons, haws, apricots, fructus hippophae, Hami melons, green jujubes, mangosteens, grapefruits, persimmons, chayote, fresh gingers, chrysanthemum coronarium, white fungus, abelmoschus esculentus and lucid ganoderma. The enzyme stock solution is good in mouthfeel and can enhance human body resistance.