3239 results about "Probiotic bacteria" patented technology

The invention relates to the technical field of biological medicines, and discloses a high-activity lactobacillus rhamnosus probiotic microcapsule, which comprises the following components in parts by weight: lactobacillus rhamnosus: each gram of formula contains 1010 CFU; bifidobacterium: each gram of the formula contains 109 CFU; 10% to 20% of sodium alginate; 5%-15% of chitosan; 3%-8% of hydroxypropyl methyl cellulose; 3%-7% of pectin; 5% to 10% of fructo-oligosaccharide; 5%-10% of inulin; 0.1%-0.5% of vitamin C; and 0.1%-0.3% of vitamin E. Through double-layer and three-layer embedding technologies (such as compound use of sodium alginate and chitosan and compound use of pectin and gelatin), the tolerance of the probiotics in gastric acid and bile environments is remarkably improved, the probiotics are effectively protected through a multi-layer embedding structure, the survival rate of the probiotics in the gastric environment reaches up to 90%, and the release rate in the small intestine exceeds 75%.

The invention discloses a method for detecting probiotics in fermented rotten vegetable feed based on the Internet of Things, and relates to the technical field of microbiological detection, and the method comprises the following steps: obtaining complete echo signal time sequence data, and carrying out high-frequency sampling on an echo signal time sequence by a sliding time window with a fixed length, and constructing a three-dimensional optical signal transient feature vector. According to the method, the transient feature vector of the three-dimensional optical signal is constructed, and a disturbance residual error and time sensitive weight mechanism is introduced, so that the abnormal disturbance of the optical signal is accurately recognized; by combining the optical interference index and the dynamic sampling window adjustment strategy, the refractive index mutation area is automatically avoided, the sensing misjudgment and data distortion are avoided, the detection stability and accuracy of the probiotic activity state in the fermentation environment are remarkably improved, and the intelligent monitoring capability of the feed quality is enhanced.

The invention discloses a probiotic with the depression improving effect and a microecological preparation and application thereof, and is characterized in that the probiotic comprises lactobacillus mucus PC17 with the preservation number of CGMCC No.33866, the active ingredients of the lactobacillus microecological preparation comprise thalli of the lactobacillus mucus PC17, and a preparation method of the microecological preparation comprises the following steps: adding the lactobacillus mucus PC17 into the lactobacillus mucus PC17, uniformly mixing the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17, the lactobacillus mucus PC17 and the lactobacillus mucus PC17 The lactic acid bacteria are inoculated into a fermentation culture medium according to a volume ratio of 1-5% to prepare the lactic acid bacteria microecological preparation, can be used for preparing antidepressant drugs, and has the advantages of being capable of promoting RIN14B cells to secrete 5-hydroxytryptamine and 5-hydroxytryptophan, reducing the levels of interleukin-1beta, interleukin-6, tumor necrosis factor-alpha, corticosterone and corticotropin in serum, and improving the antidepressant activity of the antidepressant drugs. The levels of aminobutyric acid and brain-derived neurotrophic factors in serum are improved, and the depression-like behaviors of CUMS depression model mice are obviously improved.

The invention relates to the technical field of microorganisms, and discloses a multifunctional compound probiotic composition which comprises the following raw materials in percentage by mass: 40-50% of probiotics; 30%-40% of a microcapsule embedding material; prebiotics: 10%-25%; 1%-5% of auxiliary materials; the invention also provides a preparation process of the multifunctional compound probiotic composition. The preparation process comprises the following steps: S1, culturing the probiotics and detecting the activity; s2, a microencapsulation process: preparing a multi-layer microcapsule through a spray drying method and an emulsion crosslinking method, and embedding the probiotics; and S3, mixing the microcapsules with prebiotics and auxiliary materials to obtain the multifunctional compound probiotic composition. By adopting a composite probiotic compatibility technology and through the synergistic effect of different strains, the stability and functionality of intestinal microecology are enhanced, and the effects of improving the survival rate of probiotics in gastrointestinal tracts, promoting planting of beneficial bacteria and enhancing the immunoregulation ability are achieved.

The invention relates to the technical field of microorganisms, in particular to application of pediococcus acidilactici. The pediococcus acidilactici TG005 is preserved in the China Center for Type Culture Collection on June 24, 2024, the preservation number is CCTCC NO: M 20241349, and the preservation address is Wuhan University, Wuhan, China. The pediococcus acidilactici TG005 provided by the invention is a multifunctional probiotic strain, is obtained by screening from domestic healthy human excrement samples, and has good application safety and functional adaptability; the strain can reduce the cholesterol level and prevent cardiovascular diseases; uric acid precursor substances are degraded, uric acid generation is reduced, and hyperuricemia and related diseases are prevented; pathogenic bacteria with fusobacterium nucleatum and bacteroides fragilis can be inhibited, and colorectal cancer is prevented; when being used as a probiotic additive for preparing medicines for degrading cholesterol, preventing cardiovascular diseases caused by high cholesterol, preventing hyperuricemia, colorectal cancer and the like, the lactobacillus plantarum has a wide application prospect.

The present invention provides a Lacticaseibacillus rhamnosus UP180, and its preservation number is CGMCC NO.32109. The present invention also provides a fermentation product obtained by fermenting astragalus extract by the Lacticaseibacillus rhamnosus UP180. The present invention also provides a probiotic preparation, including the Lacticaseibacillus rhamnosus UP180, which helps to promote growth and development. The present invention also provides a preparation method of the probiotic preparation, including the steps of fermenting and culturing the probiotics to obtain bacterial mud, and freeze-drying the bacterial mud after mixing it with a freeze-drying protective agent. It is convenient for subsequent industrial applications.

The invention provides a probiotic enteric dry suspension as well as a preparation method and application thereof, and relates to the technical field of pharmaceutical preparations. The probiotic enteric dry suspension comprises the following components in parts by mass: 1-8 parts of probiotic powder; 25 to 60 parts of a microcapsule cross-linking system; 1-15 parts of a suspending aid; 20 to 70 parts of a filling agent; 0-0.1 part of a coloring agent; and 0-1 part of an aromatic. According to the invention, when the probiotic powder is dispersed in water by utilizing the gelling property of sodium alginate, the probiotic powder is wrapped to form a probiotic-sodium alginate compound, citric acid and calcium carbonate are subjected to an acid-base reaction, and generated carbon dioxide bubbles can prevent the probiotic-sodium alginate compound from agglomerating; meanwhile, the released calcium ions and chitosan interact with the probiotic-sodium alginate compound, a compact probiotic microcapsule structure is rapidly and stably formed in one step, the probiotics in the microcapsule are effectively protected from being damaged by gastric acid and bile, the survival rate of the probiotics is increased, and the enteric effect is achieved.

The invention relates to the technical field of oral cavity cleaning and nursing materials, and discloses an oral cavity nursing composition containing plant extract components, which comprises plant extract components, inactivated probiotics, prebiotics and fluoride. The preparation method of the toothpaste product comprises the following steps: step 1, weighing corresponding components according to a formula, adding the cortex magnoliae officinalis extract, the lactobacillus paracasei, the fructo-oligosaccharide, the sucralose, the sodium benzoate and the sodium fluoride into the deionized water, stirring to fully dissolve, then adding the glycerol and the sorbitol, and stirring uniformly to obtain a mixture 1; fully mixing silicon dioxide, cellulose gum, hydroxyethyl cellulose and sodium lauryl sulfate to obtain a mixture 2; compared with the prior art, the oral cavity cariogenic bacteria inhibiting composition has the advantages that various oral cavity cariogenic bacteria are inhibited safely and effectively by using plant extract components, and beneficial bacteria proliferation is promoted by inactivating probiotics agglutinating harmful bacteria and prebiotics, occupation in oral mucosa is increased, colonization of harmful bacteria is reduced, so that the anti-sugar effect is achieved, and caries are prevented.

The invention belongs to the field of food, and particularly relates to a nutritional composition containing probiotics and breast milk oligosaccharide for improving anxiety or depression and application of the nutritional composition. The invention provides application of a nutritional composition in preparation of food beneficial to improving constipation and anxiety state and / or depression state caused by constipation. Wherein the nutritional composition comprises the following components (i) and (ii): (i) a bifidobacterium probiotic, and (ii) a sialylated breast milk oligosaccharide; the bifidobacterium probiotics at least comprise animal bifidobacterium subsp. Lactis, the sialylated breast milk oligosaccharide at least comprises 6 '-sialylated lactose, and the animal bifidobacterium subsp. Lactis comprises a Bb-12 strain. The substance combination of the bifidobacterium probiotics and the sialylated breast milk oligosaccharide can synergistically improve abnormal brain-intestine interaction, and improve constipation and anxiety and / or depression state caused by constipation.

The invention relates to pediococcus pentosaceus with a cholesterol lowering function and application thereof, and belongs to the technical field of microorganisms. The invention discloses pediococcus pentosaceus with a cholesterol lowering function and application of the pediococcus pentosaceus. The pediococcus pentosaceus R5 (GDMCC No.65659) is separated from special fermented food stinky acid in Guizhou. The in-vitro cholesterol removal rate of the pediococcus pentosaceus R5 can reach 59%, and the pediococcus pentosaceus R5 has the characteristics of acid / cholate resistance, no hemolysis and sensitivity to common antibiotics; an in-vivo lipid-lowering experiment is carried out on a hyperlipidemia model mouse, and the result shows that the strain R5 can remarkably improve the in-vivo dyslipidemia state of the mouse, reduce liver fat accumulation and protect the liver at the same time, and can be used for developing lipid-lowering related probiotic functional products. The strain R5 provided by the invention provides a natural and safe probiotic for preventing and / or treating hyperlipemia.

The invention discloses a probiotic fermentation device, and relates to the technical field of fermentation devices, the probiotic fermentation device comprises a fermentation tank, a tank cover is mounted on the fermentation tank, a fixed plate is fixedly connected to the inner wall of the fermentation tank, a gas collection plate is slidably connected to the inner wall of the fermentation tank close to the upper part of the fixed plate, and a ventilation shaft is rotatably connected to the middle part of the tank cover; the middle part of the gas collecting plate is slidably connected to the outer surface of the ventilation shaft, and the outer surface of the middle part of the ventilation shaft is rotatably connected to the fixed plate; compared with the prior art, the device has the advantages that the ventilation shaft rotates under the action of driving force, the ventilation stirring plate can be driven to move together, comprehensive stirring operation is carried out on mixed thalli in the fermentation container, uniform distribution of the thalli is promoted in the stirring process, the contact opportunity of the thalli and oxygen is greatly increased, and the fermentation efficiency is improved. Therefore, the fermentation rate and efficiency of the probiotics are obviously improved.

The invention belongs to the technical field of biological medicine, and discloses a probiotic agent capable of improving the physiological activity function of vitamin D and maintaining bone health and application of the probiotic agent. The invention provides a probiotic agent composed of bifidobacterium adolescentis and bifidobacterium longum, the probiotic agent can significantly improve the bone mineral density, the bone trabecula thickness, the bone trabecula number, the bone trabecula connectivity and the bone volume fraction of an osteoporosis mouse, and meanwhile, the bone trabecula separation degree of the osteoporosis-removed mouse is significantly reduced; the level of vitamin D metabolites such as 25-hydroxy vitamin D and 1, 25-dihydroxy vitamin D in mouse serum is improved; the content of serum calcium and alkaline phosphatase in serum of mice is reduced, and the level of osteocalcin and type I procollagen amino terminal peptide in the serum is improved. The probiotic agent can relieve bone calcium loss, maintain bone health, repair bone injury, increase osteoblasts and reduce the osteoporosis degree, and can be used for preparing drugs for preventing and / or treating osteoporosis or preparing health care products or food beneficial to improving bone mineral density.

The invention discloses humanized III-type collagen as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. According to the method, firstly, on the basis of an original humanized III-type collagen gene, cell adhesion activity is taken as an evaluation index, a better cell adhesion sequence is screened out and spliced and integrated with the original collagen gene, and brand-new humanized III-type collagen is obtained. And constructing a recombinant expression vector of the humanized III type collagen containing the adhesion sequence by taking the probiotic EcN as a host cell, and introducing the recombinant expression vector into the EcN to obtain a strain of functional recombinant probiotic. Compared with a traditional escherichia coli engineering bacterium BL21 (DE3), the recombinant probiotics have good cell adhesion activity while efficiently expressing the humanized type III collagen, the bacterial endotoxin content of the recombinant humanized type III collagen obtained through purification meets the industrial standard, and the recombinant probiotics have good application prospects. The purification cost caused by industrial removal of endotoxin can be avoided, so that the production cost is reduced, and the method has a good application prospect.

The present invention relates to a primer set for discrimination of bacterial species in a probiotic composition and a method for discriminating bacterial species using the same. Specifically, in the present invention, a primer set targeting the ITS (internal transcribed spacer) region between the 16S rRNA and 23S rRNA of probiotic species and pathogenic bacterial species has been constructed, it has been confirmed that 21 probiotic species and 4 pathogenic bacterial species can be discriminated with high resolution and accuracy by performing metagenomic analysis of a probiotic composition using the same, and the primer set can be usefully used to discriminate bacterial species in a probiotic composition.

The invention discloses bifidobacterium animalis subsp. Lactis, a preparation and application of the bifidobacterium animalis subsp. Lactis in regulation of depression and intestinal flora. Belongs to the technical field of probiotic preparation. According to the application, seven strains of bacteria are separated from excrement of infants, the seven strains of bacteria comprise bifidobacterium breve, bifidobacterium animalis and bifidobacterium longum through identification, the anti-depression capacity of the seven strains of bacteria is evaluated through in-vitro experiments, a potential antidepressant bifidobacterium animalis subsp. Lactis BD1 is screened out, and further experiments prove that the antidepressant bifidobacterium animalis subsp. Lactis BD1 has the advantages that the antidepressant bifidobacterium animalis subsp. Lactis BD1 can be used for preparing the antidepressant bifidobacterium animalis subsp. Lactis BD1; the strain has the effects of improving depressive mood, intestinal bacteria unbalance and insufficient serotonin secretion in the brain caused by chronic bound stress (CRS), and compared with bifidobacterium animalis subsp. Lactis in the prior art, the strain achieves the anti-depression effect from multiple aspects, also has the effect of adjusting intestinal flora imbalance, and has the advantages of being safe and reliable. And a new treatment strategy is provided for depression and intestinal flora imbalance.

The invention provides a composition capable of penetrating an intestinal barrier by oral administration. The composition contains a carrier or an auxiliary material of a targeted Microflod cell Dectin-1 receptor, and one or / and a plurality of medicinal components / components, wherein the carrier or the auxiliary material of the target Microflood cell Dectin-1 receptor is beta-glucan, and the carrier or the auxiliary material of the target Microflood cell Dectin-1 receptor is beta-glucan. The effective components / components are traditional Chinese medicine effective components / components, or / and probiotics, or / and chemical medicine components, or / and biological medicines. The invention relates to a composition capable of realizing oral administration and penetrating through an intestinal barrier, which is based on the discovery of the inventor that the composition reaches colon through gastrointestinal tracts, targets colon Microflood cells, is taken by the Microflood cells, penetrates through the intestinal barrier and enters immune tissue Colonic Patches of a colon submucosa. Based on the research, the composition disclosed by the invention can be used for treating various diseases, such as ulcerative colitis, Crohn's disease, colon cancer, rheumatoid arthritis or bacterial pneumonia after being orally taken and absorbed by rectum.

The invention relates to the field of hygienic products, and particularly discloses a probiotic hygienic product and a preparation method thereof. The probiotic hygienic product comprises a probiotic non-woven fabric, the probiotic non-woven fabric contains fibers rich in probiotics, and a preparation method of the fibers rich in the probiotics comprises the following steps: dissolving soybean protein isolate and calcium chloride to obtain a receiving solution; performing coaxial electrostatic spraying on the shell layer solution and the core layer solution to obtain probiotic embedded particles; carrying out electrostatic spinning on polyethersulfone to obtain a polyethersulfone nanofiber membrane; adding the probiotic embedded particles into a pectin solution to obtain a spraying solution, and spraying the spraying solution on the polyether sulfone nanofiber membrane to obtain blended powder; and mixing the blended powder with PE resin and an auxiliary agent according to a mass ratio, extruding, granulating to obtain a shell material, and carrying out composite spinning on the shell material and PET slices. The probiotic hygienic product disclosed by the invention has the advantages of high survival rate of probiotics, good storage stability, high activity retention rate, high water-holding capacity and good antibacterial activity.

The invention provides a combined fermented product of ginseng, astragalus membranaceus and donkey-hide gelatin as well as a preparation method and application of the combined fermented product, and relates to the technical field of pharmaceutical preparations. The combined leavening is obtained by fermenting ginseng, astragalus membranaceus and donkey-hide gelatin, and the mass ratio of the ginseng to the astragalus membranaceus to the donkey-hide gelatin is (1-3): (10-30): 1. The composition of ginseng, astragalus membranaceus and colla corii asini is subjected to probiotic fermentation, macromolecular saponin is converted into aglycone or secondary saponin with smaller molecules, protein is split into peptide chains with smaller molecules, polysaccharide is split into sugar chains with smaller molecules, absorption and utilization of effective components of the medicine are facilitated, and the effect of tonifying qi and blood can be rapidly achieved.

The invention belongs to the technical field of microorganisms, and provides streptococcus salivarius subsp. Thermophilus HX-ST36, and the preservation number of the streptococcus salivarius subsp. Thermophilus HX-ST36 is CGMCC NO.32955. The streptococcus salivarius subsp. Thermophilus provided by the invention can be used for fermenting astragalus membranaceus-angelica sinensis. In a cold coagulation and blood stasis type primary dysmenorrheal model mouse, a fermentation product can remarkably inhibit the writhing reaction, and the effects of relieving dysmenorrheal and enriching blood can be achieved by reducing the levels of prostaglandin PGF2 and vasopressin ADH in uterine tissue and increasing the content of hemoglobin (HGB). The dampness clearing effect of the fermentation product is verified by improving the heavy physical sign of moisture through crowd experiments. The invention also provides a preparation method of the probiotic preparation and the probiotic fermentation product. The preparation methods of the probiotic preparation and the probiotic fermentation product are simple, and large-scale production can be realized.

The present disclosure relates to the identification of a group of microorganisms, which are relatively abundant in the microbial communities associated with fruits and vegetables typically consumed raw and therefore transient or permanent members of the human microbiota. These microbes are used to augment the effects of additional probiotic strains. The consumption of mixtures of these microbes at relevant doses will produce a beneficial effect in the host. Therapeutic methods of the disclosure involve the use of live microorganisms or metabolites derived from said microorganisms to establish a microbial composition in the mammalian host that will provide a health benefit to a mammal in need thereof.

The invention discloses a complex microbial inoculant and application thereof, and belongs to the technical field of microorganisms. The composite microbial agent comprises bacillus (bacillus sp.BS-K1) and pseudomonas sp.CN-B1), and the preservation number of the bacillus is CCTCC (China Center for Type Culture Collection) NO: M 20242022, and the preservation number of the pseudomonas is CCTCC NO: M 20242022. The preservation number of the pseudomonas is CCTCC (China Center for Type Culture Collection) NO: M 20242021. According to the complex microbial inoculant provided by the invention, probiotics (Bacillus sp.BS-K1) and denitrifying bacteria (Pseudomonas sp.CN-B1) which have antibacterial effects are compounded to obtain the complex microbial inoculant for aquaculture, and when the complex microbial inoculant is applied to an aquaculture water body, the complex microbial inoculant has excellent denitrification capability and an inhibition effect on related pathogenic bacteria in the water body; the removal efficiency of ammonia nitrogen and nitrite nitrogen in the culture water body can be improved, the number of related pathogenic bacteria is reduced, and the safety of the culture environment is guaranteed.

The invention discloses a probiotic packaging stability control method based on deep learning, and the method comprises the following steps: collecting multi-source data in a packaging process, and carrying out the preprocessing of the multi-source data; performing time sequence feature analysis on the standardized structured data set, and extracting multi-scale time sequence features; performing target segmentation and multi-dimensional structure feature extraction on the standardized microscopic image data set; feature fusion is carried out, a probiotic packaging stability feature space is constructed, and a stable feature vector is generated; inputting a time sequence prediction network, and carrying out time sequence modeling and prediction processing; performing control parameter adjustment and constraint optimization based on a difference value between a prediction result and stability reference data; and executing and updating the packaging control adjustment scheme according to feedback to complete a packaging control closed loop. According to the method, deep learning and multi-source data analysis are fused, intelligent prediction and adaptive control of the probiotic packaging process are realized, and the method has the advantages of high stability and high precision.

The invention relates to the field of biological medicines, in particular to a composition containing pyruvate as well as a preparation method and application of the composition. The composition containing pyruvate is prepared from the following raw materials in percentage by mass: 0.05 to 0.1 percent of pyruvate, 0.02 to 0.04 percent of bergenin derivative, 5 to 10 percent of water for injection and the balance of propellant, the structural formula of the bergenin derivative is as follows: # imgabs0 #. The composition containing pyruvate can reduce the abundance of pathogenic bacteria and increase the abundance of probiotics, so that the effect of treating lung diseases is achieved by adjusting the respiratory tract micro-ecology.

The invention discloses a metal ion-mediated composite polysaccharide-based steady-state probiotic loading body as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. Sodium alginate and metal ions are coordinated and self-assembled through interaction of non-covalent supramolecules to form a metal ion-alginate skeleton network, functional guar gum is further filled, nano-scale aggregates are pushed to be spontaneously deposited on the surfaces of thalli, and a compound polysaccharide coating with excellent adhesion performance and steady-state performance is generated, so that the stability of the composite polysaccharide coating is improved. The independent coating of the probiotics is realized. The presence of metal ions increases the affinity of the biological material to the coated probiotics, and guar gum endows the probiotic delivery system with good adhesion characteristics. According to the probiotic loading body, the operation steps of an existing cell surface modification technology are simplified, the density is improved while the usage amount of biological materials is reduced, cells can be protected from being damaged by the gastrointestinal tract stress environment so as to maintain the activity of probiotics, and the colonization capacity and the biological activity of the probiotics in the intestinal tract are improved.

Provided is the use of a conjugated cholic acid, tryptophan-conjugated bile acids (TrpCA) or a TrpCA-producing strain for preventing and / or treating a metabolic disease. The present disclosure relates to the use of tryptophan-conjugated cholic acid or its producing bacteria strain in the preparation of a composition for preventing and / or treating a metabolic disease, or for promoting GLP-1 and / or insulin production. The inventors have discovered for the first time that the tryptophan-conjugated cholic acid can significantly promote the secretion of GLP-1 in both in vivo animal model and in vitro cell model, thereby reducing weight, lowering blood glucose and relieving symptoms of non-alcoholic fatty liver disease. Moreover, colonization of the selected probiotics can also have the same metabolic improvement effects.

According to the probiotic agent for enhancing the immunity and improving the intestinal flora and the application of the probiotic agent, five specific different types of bifidobacterium strains are compounded, potential interaction exists among the five strains, and the five strains can cooperate with one another, so that the effect of enhancing the immunity and improving the intestinal flora is achieved. The bifidobacteria has the advantages that the bifidobacteria has synergistic interaction in the aspects of promoting the growth of immune organs and increasing the total quantity and diversity of intestinal flora, the bifidobacteria is matched with basic fermentation strains for yogurt fermentation, meanwhile, the composite prebiotics are added for synergistic yogurt fermentation, the quantity of the bifidobacteria at the end of a shelf life after the yogurt fermentation is finished is still not less than 1 * 10 < 8 > CFU / mL or 1 * 10 < 8 > CFU / g, and the content of the bifidobacteria is not less than the stability of lactic acid bacteria in the yoghourt is remarkably improved, so that the number of viable bacteria which enter the intestinal tract of a human body and play roles is remarkably increased, and the yoghourt has relatively good tolerance in the intestinal tract; the prepared yoghurt is fine and smooth in texture, rich in taste and rich in fragrance; in addition, the immune decline and intestinal flora disorder caused by cyclophosphamide (CTX) can be remarkably improved, and a positive effect on immunological enhancement is achieved.

The invention discloses a protease preparation for preventing pet hair ball disease as well as a preparation method and application thereof, and belongs to the technical field of pet nutrition and health. The protease preparation for preventing the pet hair ball disease is mainly prepared from a compound enzyme, vitamins, trace elements, dietary fibers, probiotics, meat powder and a wall material, and the mass ratio of the compound enzyme to the vitamins to the trace elements to the dietary fibers to the probiotics to the meat powder to the wall material is (1-100): (0.1-1): (0.1-10): (1-70): (0.1-10): (10-60): (10-60). The protease preparation provided by the invention can effectively degrade hair and indigestible food, prevents the hair and food residues from forming pellets to block gastrointestinal tracts, prevents hair bulb syndrome from occurring in mechanism, and is simple in preparation process and wide in application prospect.

The invention relates to a probiotic agent for regulating an intestinal environment and an application of the probiotic agent. The bacterial strains in the probiotic agent for regulating the intestinal environment comprise a Bifidobacterium animalis subsp. Lactis C-1 bacterial strain and a Lactobacillus gasseri CKCC 1913 bacterial strain, and the bacterial strains in the probiotic agent for regulating the intestinal environment comprise a lactobacillus plantarum subsp. Lactis C-1 bacterial strain and a lactobacillus gasseri CKCC 1913 bacterial strain, a lactobacillus plantarum subsp. Lactis CKCC 1913 bacterial strain, a lactobacillus plantarum subsp. Lactis CKCC 1913 bacterial strain and a lactobacillus plantarum subsp. Lactis CKCC 1913 bacterial strain. A synergistic interaction relationship exists between the two bacteria, so that the intestinal micro-ecological environment can be regulated, the intestinal injury can be relieved and the intestinal barrier function can be improved by reducing the level of inflammatory factors, balancing the content of gastrointestinal regulatory peptides, promoting intestinal peristalsis and other action mechanisms. Therefore, the probiotic agent is suitable for preparing a preparation with the efficacy of preventing, improving or treating constipation and other inflammatory diseases.

The present invention relates to the screening of functional probiotics and its application, and specifically provides a strain of Lactobacillus casei RH99 that produces high extracellular polysaccharides and relieves vaginitis, and its preservation number is CGMCC No.32108. The Lactobacillus casei can inhibit the growth of Gardnerella, Candida albicans, and Peptostreptococcus and their biofilm generation ability; the extracellular polysaccharides produced by it have a significant promoting effect on the biofilm formation of Lactobacillus casei itself. RH99 is inoculated into the vagina, and its strain, the metabolites (exocellular polysaccharides) produced, and the core monosaccharide (D-mannitol) can all inhibit the colonization of pathogenic bacteria and promote the colonization of lactic acid bacteria; and RH99 can also relieve vaginitis by inhibiting inflammatory factors. It can be seen that RH99 can inhibit the growth of pathogenic bacteria + inhibit biofilm formation + inhibit pathogenic bacteria adhesion + inhibit the level of inflammatory factors through key core metabolites, and realize the all-round inhibition of four-in-one.

The invention provides application of combined cholic acid TrpCA and producing bacteria thereof in treating and preventing metabolic diseases. The present invention relates to the use of tryptophan-binding cholic acid or a producing bacterium thereof in the preparation of a composition for preventing and / or treating a metabolic disease or for promoting the production of GLP-1 and / or insulin. The inventor finds for the first time that tryptophan-bound cholic acid can remarkably promote secretion of GLP-1 on an in-vivo animal model and an in-vitro cell model, the effects of reducing weight, reducing blood sugar and relieving the symptoms of the non-alcoholic fatty liver disease are achieved, and the same metabolism improvement effect can also be achieved through planting of probiotics capable of generating cholic acid TrpCA.