1162 results about "Probiotic bacteria" patented technology

The invention discloses a probiotic packaging stability control method based on deep learning, and the method comprises the following steps: collecting multi-source data in a packaging process, and carrying out the preprocessing of the multi-source data; performing time sequence feature analysis on the standardized structured data set, and extracting multi-scale time sequence features; performing target segmentation and multi-dimensional structure feature extraction on the standardized microscopic image data set; feature fusion is carried out, a probiotic packaging stability feature space is constructed, and a stable feature vector is generated; inputting a time sequence prediction network, and carrying out time sequence modeling and prediction processing; performing control parameter adjustment and constraint optimization based on a difference value between a prediction result and stability reference data; and executing and updating the packaging control adjustment scheme according to feedback to complete a packaging control closed loop. According to the method, deep learning and multi-source data analysis are fused, intelligent prediction and adaptive control of the probiotic packaging process are realized, and the method has the advantages of high stability and high precision.

The invention belongs to the technical field of microorganisms and fermentation engineering, and particularly relates to application of bifidobacterium longum subsp. Infantis Y46 in the anti-aging aspect. The bifidobacterium longum subsp. Infantis Y46 is obtained from excrement of infants. Animal experiments prove that living cell thalli (Y46) and thermally inactivated cell thalli (HKY46) obtained by culturing the bifidobacterium longum subsp. Infantis Y46 by using an MRS liquid culture medium containing L-cysteine hydrochloride (0.05%, v / v) can significantly reduce accumulation of lipofuscin in a host, and prolong the healthy life and life of the host. Therefore, the bifidobacterium longum subsp. Infantis Y46 is a promising probiotic and a promising metagen with an anti-aging function.

The invention discloses a method for extracting eucommia ulmoides with an intestinal repair function, and relates to the technical field of natural product extraction.The method comprises the steps that a fresh eucommia ulmoides raw material is subjected to low-temperature drying and smashing, a polysaccharide water extract and filter residues are obtained through water extraction, and then the flavone residual quantity in the polysaccharide water extract is synchronously detected; carrying out enzymolysis on the polysaccharide water extract through beta-glucanase to obtain a small molecular polysaccharide solution, and carrying out alcohol extraction on filter residues to obtain a flavone alcohol extract; the preparation method comprises the following steps: mixing the two components according to a ratio, inoculating compound probiotics for anaerobic fermentation, regulating according to the residual quantity of flavone, regulating an alcohol extraction process by using short-chain fatty acid generated by fermentation when the residual quantity is high, supplementing flavone extract in an enzymolysis system when the residual quantity is low, and finally removing impurities, purifying, adapting intestinal tracts and drying to obtain the product. According to the method, regulation and control operation is correspondingly executed by detecting the residual quantity of flavone in the polysaccharide water extract, so that self-supply of a regulation and control reagent and high efficiency of active ingredients are realized, the conversion rate of small molecular polysaccharide and flavone aglycone is increased, and the intestinal tract tolerance of the product is optimized.

The invention belongs to the technical field of preparation of probiotics, and provides a bacterial strain with anti-allergic and immunity-improving effects and application of the bacterial strain. According to the method, a three-layer physical self-assembly structure is adopted, and polysaccharide-polyphenol layer-by-layer self-assembly coating treatment, lipid insertion layer treatment and calcium ion crosslinking protection coating treatment are sequentially carried out. And mannan oligosaccharide MOS and / or galactooligosaccharide GOS are / is mixed in the chitosan layer in a ratio of 0.05-0.15% w / v, and the surface sugar ligand density reaches 4-8 [mu] g per 10 CFU. The coating is constructed under the condition that the pH value is 5.4-5.8; the thickness of the lipid layer is 20-80 nm when the pH value is 5.6-6.2; the cross-linked layer is treated at the pH of 6.0-6.5, so that the gastric acid environment is stable for 2 hours, and the gastric acid is controllably released in intestinal juice for 2-4 hours. The prepared strain has the total thickness of 220-900 nm, the outermost layer coverage rate is larger than or equal to 85%, the gastric juice survival rate is remarkably improved, the strain can be prepared into an oral preparation to be used for preventing and relieving allergic diseases, the viable count of each dose is 1 * 10 <-1 > * 10 <-1 > CFU, and the process is mild and suitable for industrial production.

The present invention relates to the use of probiotics, preferably one or more probiotic bacteria, to increase the absorption of proteins or the bioavailability thereof, preferably in individuals with increased protein and / or energy requirements, preferably elderly persons, children, pregnant women or athletes.

The invention provides a genetically engineered bacterium for producing polydeoxyribonucleotide as well as a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium comprises kluyveromyces marxianus for expressing a salmon DNA polymerase I gene. According to the invention, the edible probiotic Kluyveromyces marxianus is transformed through genetic engineering, efficient, safe and controllable production of PDRN is realized, the biological activity of PDRN is equivalent to that of PDRN from a natural source, and large-scale production and application of PDRN are facilitated.

The invention relates to the technical field of disease and pest control, in particular to an image recognition-based garbage dump mosquito suppression system for precisely throwing probiotics, which comprises an image acquisition and sensing module for acquiring image data, environmental parameters, mosquito characteristic data and garbage decomposition state data; the mosquito feature analysis center extracts mosquito species, density and gathering area features, and constructs a mosquito breeding risk assessment model in combination with environmental parameters; the breeding risk assessment module fuses landform, garbage distribution and mosquito activity data, assesses a mosquito breeding risk level and a key breeding area, and generates a breeding risk probability cloud picture; the probiotic delivery decision-making unit simulates inhibition effects of different probiotic formulas and delivery strategies, generates a delivery scheme, and drives the ground robot to perform targeted delivery; the self-adaptive regulation and control module monitors mosquito density change and probiotic activity in real time, and dynamically adjusts a putting scheme through a swarm intelligence algorithm. Therefore, the problems of high pollution cost, poor accuracy and the like in the prior art are solved.

The invention discloses a pet food with a synergistic anti-inflammatory function and a preparation method thereof, and the method comprises the following steps: preparing an anti-inflammatory nano-carrier, preparing an anti-inflammatory synergistic emulsion, emulsifying and dispersing active ingredients, tempering, extruding, puffing and drying, preparing probiotic particles, performing step-by-step vacuum spraying and the like. And a multi-level active component protection and delivery system is systematically constructed. Wherein the nanostructured lipid carrier and the microencapsulated ginger active extract are compounded according to a specific proportion, and a synergistic interaction system is formed through a fat source medium; and the probiotics are sprayed and added after being subjected to a multi-layer embedding and low-temperature granulation technology. The pet food prepared by the method comprises an anti-inflammatory synergistic composite unit and a probiotic particle unit, and experiments prove that the bioavailability of the core anti-inflammatory component reaches 52%, the in-vitro TNF-alpha inhibition rate reaches 65%, and the survival rate of probiotics exceeds 4.2 * 10 < 8 > CFU / g, so that the pet food is obviously superior to the traditional process, and the synergistic improvement of the anti-inflammatory effect, the component stability and the processing adaptability is realized.

This invention discloses a probiotic microcapsule and its preparation method, belonging to the field of microcapsule preparation technology. The preparation method includes the following steps: (1) dissolving Sophora japonica extract in water, stirring, adding sodium alginate, and stirring again to obtain a wall material solution; (2) centrifuging and washing the activated probiotics, preparing a bacterial suspension, mixing it with the wall material solution obtained in step (1), stirring, and obtaining a wall material bacterial suspension; (3) adding the wall material bacterial suspension obtained in step (2) to a calcium chloride solution and letting it stand, then adding the formed gel to a chitosan solution, stirring, washing, and obtaining the final product. The preparation process of this invention is simple and convenient, and the raw materials are inexpensive and easy to obtain, which can effectively reduce the production cost of microcapsules, facilitate large-scale industrial production and application promotion, effectively improve the biocompatibility, biodegradability, mechanical strength and encapsulation rate of microcapsules, and enhance the storage stability and resistance to gastrointestinal fluid stress of microcapsules.

The invention provides an application of synbiotics in preparation of a composition for improving glucagon-like peptide-1 (GLP-1). The composition comprises litchi polyphenol, probiotics and methionine. The probiotics comprise bifidobacterium animalis BCRC910645, bifidobacterium longum BCRC910812, or a combination of the bifidobacterium animalis BCRC910645 and the bifidobacterium longum BCRC910812, so that the content of the glucagon-like peptide-1 is increased.

Described herein are methods and compositions for using microbial agents (probiotics) and agents that promote growth of certain microbes (prebiotics) for management (including prevention and treatment) of musculoskeletal disorders, including osteoporosis, osteopenia, Paget's disease, stunting, osteoarthritis, osteomyelitis, and delayed or non-union fractures. Also described herein are methods and compositions for using probiotics and prebiotics for management of inflammation, and symptoms of menopause.

The invention discloses nano-enzyme coating recombinant probiotics as well as a preparation method and application thereof, and belongs to the technical field of biotechnology and nano medicine. According to the invention, engineered Escherichia coli Nissle 1917 (ECN (DE3)) is taken as a host, and glutathione peroxidase (GPX) and glutathione reductase (GR) genes are over-expressed through a genetic engineering means, so that an efficient antioxidant enzyme system is constructed. And sequentially modifying chitosan and cerium dioxide nano-enzyme (CNP) on the surfaces of the thalli by using an electrostatic layer-by-layer self-assembly technology to form a protective coating. The nano-enzyme coating significantly improves the survival rate of the recombinant probiotics in severe environments such as simulated gastric acid (pH 2.5) and bile salt (0.3%), and enhances the ability of the recombinant probiotics to remove reactive oxygen species (ROS), including hydrogen peroxide, superoxide anions, hydroxyl free radicals and DPPH free radicals.

The invention provides a probiotic composition for improving immunity and a preparation method thereof, and belongs to the technical field of probiotics. The preparation method comprises the following steps: fermenting selenium-enriched nostoc sphaeroides through selenium-enriched saccharomycetes to extract selenium-enriched nostoc sphaeroides polysaccharide and selenium-enriched nostoc sphaeroides protein peptide, mixing with traditional Chinese medicine polysaccharide obtained by extracting astragalus membranaceus, rhodiola rosea, codonopsis pilosula and pseudo-ginseng, and reacting with ferric salt to prepare a polysaccharide-Fe / selenium compound; solid residues are mixed and fermented, and active protein peptide is prepared from the solid residues and selenium-rich nostoc sphaeroides protein peptide; the probiotic composition capable of improving immunity is prepared by extracting a polysaccharide-Fe / selenium compound and a polysaccharide-Fe / selenium compound through an organic solvent, extracting a permeate through an organic solvent to obtain an active component, collecting a bacterial liquid, mixing and embedding the bacterial liquid in the polysaccharide-Fe / selenium compound and sodium alginate, and uniformly mixing the bacterial liquid with the active protein peptide, the active component, taurine and ginsenoside Rg3, so that the probiotic composition capable of improving immunity can play good anti-inflammatory, anti-oxidation, immunoregulation, anti-tumor, anti-virus and other roles, and has a good application prospect. Wide application prospects are realized.

Embodiments of the invention provide a solution for ameliorating microbiome effects on neurodevelopment by developing a probiotic composition for consumption by a female during gestation. In certain aspects the probiotic is a multi-strain probiotic cocktail consisting of immunomodulatory taxa and administered females during pregnancy and lactation.

The invention discloses a preparation process of metabiotics and functional application of the metabiotics for relieving constipation, and relates to the technical field of microorganisms. The invention provides a preparation method of a bifidobacterium animalis subsp. Lactis LIHUO 01-containing metagen, which comprises the following steps: separately inoculating bifidobacterium animalis subsp. Lactis LIHUO 01 or compounding with probiotics to inoculate in a fermentation culture medium for culturing to obtain a fermentation bacteria solution, and carrying out thermal inactivation to obtain the bifidobacterium animalis subsp. Lactis LIHUO 01-containing metagen. The fermentation medium comprises skimmed milk, soybean meal and water, the mass ratio of the skimmed milk to the soybean meal to the water is (5-30): (1-25): (78-90), and the prepared bifidobacterium animalis subsp. Lactis LISUO 01 metabolite can significantly improve the content of tryptophan metabolites, can effectively relieve constipation and has a wide application prospect.

The invention relates to the technical field of functional food, in particular to a panax quinquefolius probiotic beverage and a preparation method thereof, and the beverage comprises the following components: 30-50 parts of panax quinquefolius pulp; 20 to 40 parts of sea buckthorn raw pulp; 1 to 5 parts of marine collagen peptide powder; 1 to 5 parts of a probiotic fermentation agent; 0.5 to 3 parts of calcium beta-hydroxy-beta-methyl butyrate; 0.5 to 10 parts of an auxiliary material; the pH (Potential of Hydrogen) value of the beverage is 3.8 to 4.2. The instability of a compound system is solved by combining precise pH control, specific stabilizer compounding and a high-pressure homogenization technology, the activity of rare saponin is improved through enzymolysis conversion, the adding sequence of the raw materials is optimized, heat-sensitive components are reserved by adopting ultrahigh-pressure cold sterilization, and meanwhile, the taste is improved by blending the natural flavor of sea-buckthorn and a natural sweetening agent. Therefore, the problems of poor compounding stability, difficulty in activity retention and the like in the prior art are solved.

The invention discloses a multispectral imaging detection method and system for monitoring and judging probiotic activity based on metabolites. The method comprises the following steps: collecting a multispectral image data cuboid from a probiotic liquid preparation sample; carrying out dimensionality reduction on the multispectral image data cuboid by utilizing a principal component analysis method to obtain a characteristic spectral data matrix; clustering by using principal components in the characteristic spectrum data matrix to obtain an initial cluster; obtaining a target spectrum similarity cluster in the initial clustering cluster by using a characteristic spectrum vector in the characteristic spectrum data matrix; acquiring the intensity value of a pixel point under the characteristic wavelength for the target spectrum similarity cluster, and calculating a comprehensive activity score; presetting an activity threshold value; judging by integrating the activity score and the activity threshold value to obtain a detection result of the probiotic liquid preparation sample.

The invention discloses a biological energy feed fermentation yeast based on enzyme-mineral synergistic activation and a preparation method thereof. According to the scheme, dual functions of mineral activation and microbial conversion are focused, clinoptilolite, sodium bentonite and kaolin are taken as mineral carriers, lignite humic acid is taken as a nutrient synergist, brown sugar, rice bran and bran organic matrixes are matched, and mineral lattice structures are broken through mechanical crushing and biological enzymolysis pretreatment, so that the mineral active substances are obtained. And through a double-stage fermentation process of'aerobic fermentation to activate flora and anaerobic aging to enrich metabolites' of microorganisms, mineral ions, humic acid active ingredients and probiotic metabolites are released to the maximum extent, and finally, the high-quality biological energy feed fermentation yeast with nutrition supply, digestion promotion and immune regulation functions is formed. According to the bioenergy feed prepared by conversion of the fermentation yeast, the feed crude protein improvement rate is greater than or equal to 20%, the crude fiber degradation rate is greater than or equal to 40%, the feed conversion rate is improved by 10-15%, the intestinal disease incidence rate is reduced by 60-70%, and the bioenergy feed is suitable for various livestock and poultry and aquaculture scenes.

The invention discloses a traditional Chinese medicine-probiotic combined preparation, a preparation method thereof and application of the traditional Chinese medicine-probiotic combined preparation in gastric mucosal lesion protection. The preparation is composed of a traditional Chinese medicine extract and a bacillus subtilis bacterial suspension, the traditional Chinese medicine extract is prepared from radix codonopsis, rhizoma smilacis glabrae, rhizoma atractylodis macrocephalae, fructus aurantii, radix aucklandiae, bletilla striata, herba ecliptae, radix salviae miltiorrhizae, fructus arctii and fructus psoraleae according to a specific proportion through water decoction, filtration, freeze-drying and mixing with the prepared bacterial suspension. The combined preparation has remarkable protection and treatment effects on gastric mucosal lesion caused by helicobacter pylori infection and ethanol, not only can effectively inhibit the growth of helicobacter pylori, but also can relieve gastric inflammatory response and apoptosis, and the effect of the combined preparation is equivalent to or even better than that of a conventional triple therapy or omeprazole. Meanwhile, the preparation shows synergistic interaction functions of regulating body immunity and maintaining intestinal flora balance, the preparation process is simple and convenient, the safety is high, and the preparation has wide application prospects in preparation of drugs for resisting helicobacter pylori, protecting gastric mucosa and regulating intestinal flora.

The invention discloses Dubosiella Newyorkens TZF-001 capable of producing beta-glucosidase and application of the Dubosiella Newyorkens TZF-001 in preparation of ginsenoside through fermentation and conversion, and belongs to the field of probiotic fermentation. The Dubosiella Newyorkens TZF-001 is preserved in the China Center for Type Culture Collection on December 15, 2025, and the preservation number of the Dubosiella Newyorkens TZF-001 is CCTCC (China Center for Type Culture Collection) NO: M20252889. According to the invention, ginsenoside Rb1 is used as a raw material, and Dubosiella Newyorkens TZF-001 with high yield of beta-glucosidase is used for fermentation and conversion of ginsenoside so as to prepare ginsenoside Rd. The ginsenoside Rb1 can be completely converted into Rd after fermentation, the dependence of a traditional method on acid-base hydrolysis, high temperature and high pressure and steam treatment is avoided, the reaction condition is mild, and the method has the advantages of being environmentally friendly, low in cost, high in product purity and the like. The method opens up a brand new technical path for green and low-cost preparation of ginsenoside Rd, and has remarkable innovativeness and popularization value in the field of industrialization of active ingredients of natural medicines.

The invention relates to the technical field of microcapsule preparation, and particularly discloses a microcapsule composition with a slow release function and a preparation method and application of the microcapsule composition. Soybean protein provided by the invention is subjected to three-stage hydrolysis to generate small molecule peptide and amino acid, and the small molecule peptide and amino acid can be interwoven with maltodextrin in a network structure; therefore, a compact and coherent composite wall material network structure is jointly constructed, erosion of gastric acid can be effectively resisted, and physical isolation protection is provided for probiotics. When the microcapsule enters an environment from neutral to alkalescent intestinal tracts, the pH value in the intestinal tracts is increased, and an original hydrogen bond network structure among molecules is damaged, so that an original compact gel network structure becomes loose and is greatly swelled; meanwhile, the hydrolyzed soybean protein can be further hydrolyzed under the action of intestinal trypsin and the like, the wall material structure of the microcapsule is gradually loosened, thalli are gradually released in the swelling-disintegration process, targeted slow release of the intestinal tract is achieved, and therefore the probiotic function of the microcapsule is achieved.

The invention provides a bovine colostrum probiotic compound preparation as well as a preparation method and application thereof. The compound preparation provided by the invention comprises the bovine colostrum powder subjected to activity protection and the compound probiotics, and the bovine colostrum powder subjected to activity protection is obtained by adopting a microcapsule double-layer embedding and freeze-drying technology, so that the gastric acid retention rate (2h) of immune globulin in the bovine colostrum powder is greater than or equal to 90%, and IgG contained in the bovine colostrum powder is ensured to be efficiently delivered to intestinal tracts to play an immune regulation role. Wherein the compound probiotics comprise four stages of functional echeles formed by more than or equal to 6 strains, and the four stages of functional echeles comprise colon colonization major flora, gastric acid-resistant pioneer flora, antibacterial peptide secretion auxiliary flora and inflammation regulation backup flora. Through cooperation of the components in the compound preparation, the compound preparation has excellent immune enhancement and metabolic regulation functions.

The present application provides a probiotic microcapsule and a preparation method thereof. The probiotic microcapsule comprises a core material made of probiotics, a freeze-drying protective layer made of a freeze-drying protective agent and a wall material made of polysaccharides, wherein the freeze-drying protective layer is interposed between the core material and the wall material. The freeze-drying protective agent used in the probiotic microcapsule of the present application can reduce the damage to the bacterial body in the freezing and drying process through synergistic effect; and the wall material can form an acid-stable, dense and hard shell outside the bacterial body, greatly improving the stress resistance and stability of the probiotic in the production, storage and digestion processes. The probiotic microcapsule of the present application can be used for preparing food, cosmetics, medicines and feed.

The invention belongs to the technical field of synthesis and antibiosis of nano-enzyme catalysis systems, and discloses an enzyme catalysis system with a function of targeted inhibition of growth of streptococcus mutans as well as preparation and application of the enzyme catalysis system. The enzyme catalysis system with the targeted inhibition function on the growth of the streptococcus mutans is formed by loading AuNPs and PCN-22 (Fe) on a CMD molecular chain through in-situ growth, and the mass ratio of the AuNPs to the PCN-22 (Fe) to the CMD is (0.22%-0.33%) to (12.34%-21.79%) to (77.88%-87.44%). The cascade catalytic effect of the nano-enzyme is adjusted by regulating and controlling the carboxymethyl substitution degree of CMD, so that glucose in a decayed tooth pathological environment is efficiently catalyzed in one step to generate ROS, and damage to probiotics is reduced while the inhibition efficiency of pathogenic bacteria is improved.

The application discloses a kind of plant lactic acid bacteria, belong to fermentation drink technical field.This drink is quantified according to 1000mL product proportion, raw material includes a variety of fresh fruits and vegetables, traditional Chinese medicine component, functional component and base auxiliary material, is prepared by raw material pretreatment, mixing and fermentation, refining and packaging step.After pretreatment, mixed fruit and vegetable juice, traditional Chinese medicine compound liquid and sterile base are obtained, mixed by specific parameters, 37 constant temperature fermentation 72 hours, then multi-stage filtration and sterile packaging are made.The application innovatively constructs "fruit and vegetable nutrition+traditional Chinese medicine health+probiotic conditioning" composite system, and the efficacy is synergistic, the flavor is coordinated, the process parameter is quantified, the product stability is strong, the shelf life is 12 months, the raw material is easy to obtain and suitable for large-scale production, solves the problems that existing fermentation drink component is single, efficacy and taste are difficult to consider, etc., has the nutrition and health care value, and market application prospect is wide.

The invention discloses MoSe2 nano-enzyme-selenium-rich probiotic composite hydrogel and a preparation method and application thereof, the composite hydrogel comprises a hydrogel matrix and functional components dispersed in the hydrogel matrix, and the functional components comprise anti-MoSe2 nano-enzyme and selenium-rich probiotics wrapped by an antioxidant film. According to the MoSe2 nano-enzyme-selenium-enriched probiotic composite hydrogel provided by the invention, the sonodynamic characteristics of the MoSe2 nano-enzyme and the biological activity of the selenium-enriched probiotics are perfectly fused: under ultrasonic stimulation, the MoSe2 nano-enzyme not only can directly and efficiently generate reactive oxygen species (ROS), but also can catalyze low-concentration hydrogen peroxide to generate a large amount of hydroxyl radicals (. OH); broad-spectrum killing of various pathogenic bacteria is achieved, and the risk of drug resistance evolution of bacteria is reduced from the source; meanwhile, antibiotics secreted by the selenium-enriched probiotics can form a synergistic sterilization effect, so that the pathogenic bacterium removal effect is further enhanced.

The present invention involves an engineered probiotic bacterium comprising a heterologous nucleic acid, where the heterologous nucleic acid comprises a nucleic acid sequence encoding an anti-spike glycoprotein nanobody of a coronavirus. In one embodiment, the bacterium is Escherichia coli Nissle 1917. In another embodiment, the anti-spike glycoprotein nanobody appears on the surface of the probiotic bacteria.

The invention provides a novel ecological feed capable of preventing multiple grass-bream diseases, the novel ecological feed comprises a feed matrix and an ecological feed leavening agent, and the ecological feed leavening agent comprises probiotics with surface expression antigens, the antigen is any one or more of a grass carp reovirus antigen, a flavobacterium johnsonii antigen, an aeromonas veronii antigen and a clonorchis sinensis antigen. The novel ecological feed with a disease-resistant function in a parabramis pekinensis breeding mode is prepared for the first time, the resistance of the parabramis pekinensis to various pathogens can be enhanced, and the survival rate of the parabramis pekinensis in the disease attack peak period can be increased by 50% or above after immunization. An oral immunization mode is adopted and can be completed in the feeding process, and the immunization and breeding cost is greatly saved.

The invention relates to preparation of fermented milk, in particular to probiotic fermented milk and a preparation process thereof, and the process comprises the following steps: step 1, connecting the fermented probiotic fermented milk to a canning pipe through a pipeline; 2, the canning container is horizontally placed on a transfer belt, and a canning pipe is horizontally inserted into the canning container; thirdly, the conveying belt drives the canning container to move, and the two limiting rods limit and straighten the canning container, so that the canning container is changed from the horizontal state to the vertical state; fourthly, the canning pipe moves along with the movement of the canning container, and canning is completed; the filling process of the probiotic fermented milk can be stably completed, and bubbles are reduced.

The invention provides a breeding method suitable for breeding litopenaeus vannamei in saline-alkali soil, and relates to the technical field of aquaculture. According to the breeding method suitable for breeding the litopenaeus vannamei in the saline-alkali soil, the proportion of Ca < 2 + > to Mg < 2 + > to K < + > in a water body is optimized to be 2.8: 1: 0.3 by customizing a compound ion regulation and control solution, SO4 < 2-> / Cl <-> is controlled to be 0.14-0.8, and the pH is stabilized to be 8.2-8.8 in combination with a citric acid-sodium citrate buffer system; a slow-medium-fast segmented domestication strategy is adopted to adapt to different development stages of the fries; ethanol clostridium protein (CAP) is added into the feed to be combined with compound probiotics, so that immune metabolism regulation and control are enhanced. Experiments prove that according to the method, the survival rate of the litopenaeus vannamei seedlings reaches 86.3% + / -2.1%, the culture period is shortened by 12-15 days, the hepatopancreas SOD activity is improved by 42.6%, the vibrio inhibition rate reaches 78.5%, and the method is remarkably superior to the prior art. The method does not need large-scale pond transformation, adapts to different types of saline-alkali soil water bodies, realizes efficient green culture, and has important application value.