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Application of bacteroides fragilis in preparation of composition for treating inflammatory bowel diseases

A technology for Bacteroides fragilis and inflammatory bowel disease, which is applied in the application field of Bacteroides fragilis to achieve the effects of strong pharmacological effects, good therapeutic effects, good food and medicinal prospects

Inactive Publication Date: 2013-06-19
广东知光生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Bacteroides fragilis is used as a kind of probiotics, and its related application in the preparation of compositions for treating inflammatory bowel disease has not yet been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Preparation of Bacteroides fragilis bacterial liquid

[0019] (1) Isolation of Bacteroides fragilis from infant feces

[0020] Weigh 0.5 g of stool according to the "normal flora test method", put it in a test tube filled with 4.5 ml of normal saline, and serially dilute it to 10 -1 ~10 -6 , from high concentration to low concentration on the Bd medium plate, placed in an anaerobic gas box and anaerobic gas bag for anaerobic culture. Store at 37°C, open the can for inspection after 2-7 days. A single colony was selected for pure culture, staining microscopy, culture characteristics, and physiological and biochemical testing.

[0021] (2) Enrichment culture of Bacteroides fragilis and preparation of bacterial solution

[0022] Select the purified single colony and inoculate it in the improved tryptone broth for enrichment culture. The obtained bacterial solution is centrifuged and precipitated at a speed of 3000r / min for 15 minutes, and the supernatant is...

Embodiment 2

[0023] Example 2 Toxicity test of Bacteroides fragilis

[0024] The Bacteroides fragilis bacterium liquid that adopts embodiment 1 preparation carries out following experiment:

[0025] (1) Acute toxicity test in mice

[0026] Sixty C57BR / C mice weighing 22±2 g (half male and half male) were divided into high-dose group, low-dose group and control group, 20 in each group. Each mouse in the high-dose group was intragastrically administered 10 12 cfu / ml bacterial solution 0.5ml, the low dose group was administered orally 10 times a day 9 cfu / ml bacterial solution 0.5ml, and the control group was fed with 0.5ml normal saline every day. Continuous observation for 14 days.

[0027] Experimental results: The mice grew normally, no death was observed, and the LD50 was undetectable.

[0028] (2) Rat toxicity test

[0029] Take 30 SD rats (half male and half female) of 150±20 g, and divide them into 3 groups: high and low dose group and control group. Each rat in the high-do...

Embodiment 3

[0034] Example 3 Short-term therapeutic effect of Bacteroides fragilis on inflammatory bowel disease

[0035] The Bacteroides fragilis bacterium liquid that adopts embodiment 1 preparation carries out following experiment:

[0036] Experimental animal: BALB / c mouse

[0037] Animal model construction: Inflammatory bowel disease induced by chemical drugs (trinitrobenzenesulfonic acid TNBS and ethanol induced Crohn's disease, dextran sodium sulfate DSS induced ulcerative colitis)

[0038] 50 mice were randomly divided into 5 groups: TNBS group, DSS group, TNBS+Bd group, DSS+Bd group, and control group.

[0039] The mice in the TNBS group, TNBS+Bd group, and control group were fasted (without water), and the mice were lightly anesthetized by intraperitoneal injection of 50 / L chloral hydrate 0.15ml, and a small catheter was used after about 25 minutes. Aspirate the medicinal solution with a 1ml syringe in the tube, slowly insert the catheter about 3.5cm from the anus, then slow...

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PUM

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Abstract

The invention relates to the technical field of application of bacteroides fragilis, and in particular relates to application of bacteroides fragilis in preparation of a composition for treating inflammatory bowel diseases. The experiments show that bacteroides fragilis is safe and nontoxic and strong in pharmacologic action, and has good treating effect of treating inflammatory bowel diseases, thereby indicating that the bacteroides fragilis has good edible and medicinal prospects. According to the invention, a novel use of bacteroides fragilis is explored and a novel application field is developed. Bacteroides fragilis as a probiotic can be used for preparing foods or medical compositions for treating inflammatory bowel diseases so as to provide health-cared foods or treating medicines suitable for human body to take.

Description

technical field [0001] The invention relates to the technical field of application of Bacteroides fragilis, in particular to the application of Bacteroides fragilis in preparing a composition for treating inflammatory bowel disease. Background technique [0002] Inflammatory bowel disease is a chronic nonspecific intestinal inflammatory disease of unknown etiology, including Crohn's disease and ulcerative colitis. The clinical manifestations of ulcerative colitis are persistent or recurrent diarrhea, mucus pus and blood stools accompanied by abdominal pain, tenesmus, and systemic symptoms of varying degrees, and the course of the disease usually lasts for more than 4 to 6 weeks. There may be extraintestinal manifestations of skin, mucous membranes, joints, eyes, liver and gallbladder. The clinical manifestations of Crohn's disease are diverse, including gastrointestinal manifestations, systemic manifestations, extraintestinal manifestations and complications. Gastrointest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A23C9/152A23C9/127A23C19/00A23G9/36A23L1/105A23L1/30A23K1/00A61P1/00A23L7/104A61K35/741
Inventor 智发朝白杨刘洋洋
Owner 广东知光生物科技有限公司
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