The invention discloses a lysozyme soil sterilization deep ploughing-free anti-hardening root cultivation and protection microorganism fertilizer. The microorganism fertilizer comprises the following components, by weight, 25-60% of a polyacrylamide, 20-45% of calcium bentonite, 8-22% of biochemical fulvic acid, 1-3% of amino acids, and 2-5% of chelated trace elements, wherein the content of calcium in the calcium bentonite is 13-15%; in the chelated trace elements, the magnesium content is 0.5-0.8%, the zinc content is 0.2-0.8%, the copper content is 0.3-0.8%, the iron content is 0.1-0.2%, and the manganese content is 0.1-0.2%; in the amino acids, the Bacillus megaterium content is 0.05-0.15 billion/g, and the colloidal Bacillus cereus content is 0.05-0.1 billion/g; in the biochemical fulvic acid, the suborder Bacillus content is equal to or greater than 0.2 billion/g, the actinomyce content is equal to or greater than 0.2 billion/g, the Serratia carollera phosphaticum content is equal to or greater than 0.2 billion/g, and the Bacillus mucilaginosus content is equal to or greater than 0.2 billion/g. The microorganism fertilizer has the advantages of simple use, no strict requirements on environments, yield increase guarantee, fertilizer dosage reduction, cost reduction, soil and crop quality improvement, and pollution reduction.