Pseudobagrus ussuriensis genetic male reversal physiological female fish breeding sifting method

Abstract

The invention discloses a pseudobagrus ussuriensis genetic male reversal physiological female fish breeding sifting method. The method comprises the steps of incubating fry, selecting testing fry, incubating artemia, soaking artemia with an estradiol solution, regarding artemia as a hormone carrier to be fed to the fry, observing physiological genders of fingerlings and adult fish, and identifyinggenetic genders. Through optimized procedures, a proper feeding starting time, the concentration and time of the estradiol solution for soaking the artemia, the feeding lasting processing time are sifted, the genetically male individuals are genetically reversed into physiologically female individuals, male specific molecular markers are utilized to identify the genetically male physiologically female individuals to be subjected to separate-pond breeding, and breeding is conducted according to the breeding technological path of male fish sexual reversal, artificial gynogenesis, super-male fish and all-male fish. By means of the pseudobagrus ussuriensis genetic male reversal physiological female fish breeding sifting method, a large quantity of high-quality fundamental materials are provided for pseudobagrus ussuriensis all-male-fish breeding within a short period.

Description

technical field [0001] The invention relates to the field of fish biotechnology breeding, in particular to a fish breeding screening method for reversing genetic males of Ussuri catfish into physiological females. Background technique [0002] In the practice of fish farming, it has been found that there are a variety of fish with male and female dimorphism in terms of shape, growth speed, sexual maturity weight, etc. If single-row farming can not only obtain commercial fish with neat specifications, but also Improve the utilization rate of feed, increase the income of farmers, and can promote the industrialization of modern fishery and form new varieties with independent intellectual property rights, which has unique advantages and applications. Therefore, fish sex control breeding and parthenoculture are an important direction for the development of aquaculture. [0003] The most commonly used method of fish sex control breeding is to use sex hormones to induce genetic se...

AI Technical Summary

Problems solved by technology

However, because the fry are delicate and delicate, when to start treatment, how to choose the concentration of hormones during treatment, and how long the treatment lasts for the best effect, a little carelessness will cause a large number of fish fry to die, making the breeding work fail

[0005] Furthermore, whether sex hormone-induced sex reversal of fry is successful requires a test cross for deterministic verification, which generally takes 4-5 years, and it is difficult to obtain a large amount of breeding materials in a short period of time

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