257 results about "Estradiols" patented technology

The invention relates to an ovarian reserve hypofunction prediction method based on multi-data fusion, and the technical scheme comprises the following steps: regularly collecting biomarker data related to ovarian reserve, including anti-mullerian canal hormone AMH, follicle stimulating hormone FSH, luteinizing hormone LH, estradiol E2, and ultrasonic image data including follicle quantity and ovarian volume; acquiring dynamic change curve data and capturing the change trend of the ovarian reserve function when the acquisition time point covers the key stage of the menstrual cycle; analyzing the biomarker data acquired for multiple times by using an LSTM deep learning model, and modeling a change trend of the ovarian reserve function of each patient along with time; by analyzing the periodic fluctuation of AMH and FSH biomarkers, the decline time node of the ovarian reserve function in the future is predicted; and on the basis of the LSTM deep learning model of S1.1, performing personalized risk assessment in combination with individual characteristics of patients including age, genes, lifestyles and disease histories.

The invention provides a porcine oocyte in-vitro maturation culture solution additive and application thereof, and belongs to the field of embryo engineering, and the additive contains taurine. The additive also contains sodium pyruvate, cysteine, glucose, NaHCO3, fetal calf serum, estradiol, pregnant horse serum gonadotropin and human chorionic gonadotropin. When the porcine oocyte in-vitro maturation culture solution added with the additive is used for culturing porcine oocytes in vitro, the ROS content in the porcine oocytes can be remarkably reduced, the oxidation resistance of the porcine oocytes can be improved, and the mitochondrial distribution of the oocytes can be improved, so that the development of the oocytes can be improved; the maturation of porcine oocytes and the development of early embryos are promoted. When the technology is applied to the pig assisted reproduction technology, the success rate and quality of embryo engineering can be improved; the pig oocyte in-vitro maturation culture solution added with the additive can improve the premature embryo division rate in parthenogenetic activation of the pig oocytes.

The invention discloses a tree shrew depression model modeling method which comprises the following steps: (1) dissolving 20 mg of corticosterone in 10 microliters of DMSO (dimethylsulfoxide), and fixing the volume to 1 ml by normal saline; the method comprises the following steps: dissolving 100 mg of estradiol in 1 ml of DMSO (Dimethylsulfoxide) to prepare 100 mg / ml of estradiol stock solution; and (2) carrying out subcutaneous injection on the corticosterone solution prepared in the step (1) to tree shrews at 14: 00-16: 00 in the afternoon every day at the injection amount of 27 mu g / kg, diluting the estradiol stock solution prepared in the step (1) with normal saline after the corticosterone solution is injected for 1 hour, then carrying out subcutaneous injection on the tree shrews at the injection amount of 50-75 mu g / kg, and continuously injecting for 21 days to complete molding. According to the invention, a method of increasing the use dosage of corticosterone and simultaneously adding estradiol (estrogen with the highest in-vivo content and strongest activity) is adopted, so that the modeling time can be shortened. The corticosterone and the estradiol are combined for tree shrew depression modeling, so that the modeling time can be effectively shortened, and the optimal modeling effect is achieved by adopting a special estradiol dosage.

The invention provides a nucleic acid aptamer for specific recognition of morphine and application of the nucleic acid aptamer, and belongs to the technical field of biosensing and detection. The nucleotide sequence of the nucleic acid aptamer is as shown in SEQ ID NO: 1. The screening method is based on a Capture-SELEX technology and comprises the key steps that streptavidin magnetic beads are used for fixing an ssDNA library, estradiol, deabietic acid and totarol are introduced to serve as reverse screening substances so as to remove non-specific sequences, and finally the high-specificity aptamer is obtained through high-throughput sequencing and affinity determination. The dissociation constant of the aptamer and morphine is 127.31 nM, and the aptamer shows high affinity and high specificity. The invention further relates to application of the aptamer in preparation of a sensor and a kit for detecting morphine, and a new technical means is provided for rapid detection of morphine.

The invention provides a vagina foaming agent and a preparation method thereof, and belongs to the technical field of medicines. The invention provides the vaginal foaming agent taking the estradiol as the active component, the vaginal foaming agent is prepared from the estradiol, the compliance is good, the contact area in the vagina is wide, the estradiol is quickly released, the pertinence on diseases caused by lack of estrogen is strong, the effect is quick, the administration dosage is accurate, and the use is convenient.

The invention relates to the technical field of molecular biology, and particularly discloses application of miR-615 in ovarian granular cells. The miR-615 is applied to any one of the following items: preparing a product for promoting the activity and / or proliferation of ovarian granular cells; preparing a product for promoting generation of estradiol and / or progesterone; preparing a product for promoting the expression of the 3 beta-HSD and / or StAR gene; preparing a product for inhibiting ACSL4 gene expression; and preparing a product competitively combined with the lnc-GAM. According to the invention, porcine ovarian granular cells are used as a material, the expression level of lnc-GAM-miR-615 / ACSL4 regulatory axis is up-regulated or down-regulated in the porcine ovarian granular cells by using gene overexpression and interference technologies, and the expression of the lnc-GAM-miR-615 / ACSL4 regulatory axis is determined by detecting cell proliferation activity, hormone secretion level and apoptosis gene expression in combination with a dual-luciferase reporter gene test. And the regulation effect of lnc-GAM mediation on the apoptosis of the porcine ovarian granular cells is researched.

The invention belongs to the technical field of biology, and particularly relates to an anti-estradiol antibody or an antigen binding part thereof and application of the anti-estradiol antibody or the antigen binding part thereof. The antibody sequence disclosed by the invention is stable in antibody performance and high in affinity to estradiol, and has an important clinical practical value.

To provide PROteolytic TArgeting Chimeras (PROTACs) capable of inactivating a G-protein coupled estrogen receptor (GPER).SOLUTION: A molecule comprising a G-protein coupled estrogen receptor (GPER) ligand coupled to a linker coupled to an E3 ubiquitin ligase ligand and methods of using the molecule are provided. In one embodiment, the GPER ligand is estradiol and the E3 ubiquitin ligase ligand is (S,R,S)-AHPC.SELECTED DRAWING: Figure 1

The invention discloses a method for detecting 17 beta-estradiol by using a colorimetric-fluorescent dual-mode biosensor, and belongs to the technical field of chemical analysis and detection. According to the method, a colorimetric-fluorescent dual-mode biosensor for detecting 17 beta-estradiol is established based on oxidase-like activity and fluorescence quenching characteristics of manganese dioxide nanosheets and catalytic characteristics of alkaline phosphatase by taking a nucleic acid aptamer as a recognition molecule. The synthesis cost of the nucleic acid aptamer is low, the method has universality, and other target objects can be detected by changing the nucleic acid aptamer; mBs-DNA-ALP is prepared by using a biotin-streptavidin reaction and a DNA hybridization reaction, and a structure conversion process from double-stranded DNA to an aptamer / target compound is realized by using targeted induction, so that a detection signal is amplified; according to the method for detecting 17 beta-estradiol through colorimetric-fluorescence signals, dual-mode output is achieved, results are verified and supplemented mutually, and the specificity and accuracy of the method are improved.

The invention provides application of combination of antrodia camphorata polysaccharide and forsythin in preparation of drugs for treating metabolic diseases related to insulin resistance, and belongs to the technical field of biological medicines. The metabolic diseases related to insulin resistance include polycystic ovarian syndrome. Animal experiments find that the combination of the antrodia camphorata polysaccharide and forsythin can significantly reduce serum testosterone and luteinizing hormone levels of a rat with the polycystic ovarian syndrome, improve serum follicular hormone and estradiol levels, and achieve the effect of improving the polycystic ovarian syndrome.

Provided are methods for determining the amount of estradiol in a sample using mass spectrometry. The methods generally involve ionizing estradiol in a sample and detecting and quantifying the amount of the ion to determine the amount of estradiol in the sample.

The invention discloses plant lactobacillus La10 and application thereof in regulating estrogen secretion stability and enhancing ovarian function, and belongs to the technical field of microorganisms. The plant lactobacillus La10 provided by the invention can effectively promote the conversion of free isoflavone (daidzein and genistein), so that the absorption and utilization of intestinal tracts are promoted; the composition has the advantages that the ovarian index and sex hormone estradiol E2 secretion level reduction caused by premature ovarian failure of rats can be obviously improved, and meanwhile, the estrogen secretion level and ovarian index up-regulation caused by mouse hysteromyoma can be improved; the irregular menstruation phenomenon of women is improved, so that the estrogen secretion disorder is regulated. The plant lactobacillus La10 disclosed by the invention has a very wide application range in preparation of functional foods and health-care foods for promoting estrogen balance and protecting female health, and is healthy and safe.

The invention discloses an estradiol valerate sugar-coated tablet and a preparation method thereof. The estradiol valerate sugar-coated tablet sequentially comprises a tablet core, a powder coating layer, a first sugar coating layer, a coloring layer and a second sugar coating layer from inside to outside, the tablet core contains estradiol valerate; the powder coating layer is prepared from the following components in parts by weight: 1.7 to 3.7 parts of polyethylene glycol 6000, 25 to 35 parts of cane sugar, 0.1 to 0.4 part of povidone K90, 10 to 18 parts of calcium carbonate and 4 to 10 parts of talcum powder. According to the invention, crystallization of cane sugar is effectively inhibited, the surface of a powder coating layer is finer and smoother, the undesirable phenomenon of tablet edge cracking is avoided, and the quality defects of uneven color, color fading and the like caused by moisture absorption are also avoided.

The present invention relates to systems, kits, and methods for identifying subjects with increased levels (e.g., in a urine sample) of at least two urine metabolites selected from: butanal; 2-Hydroxy-1,3-dimethoxy-8,9-methylenedioxycoumestan; 6-Methylmercaptopurine; Dimethyl-L-arginine; N-butanoyl-lhomoserine lactone; Hexanoylglycine; Methyl propenyl ketone; Ferulate; 2-Oxoarginine; and 2-Hydroxyestradiol-3-methyl ether, as well as methods of determining if a subject has or is at risk of urinary stone disease based on such urine metabolites. In certain embodiments, the urinary tract of a subject with elevated levels of at least two of the urine metabolites is imaged (e.g., to generate an image showing the size, location, or number of urinary stones present).

The present invention relates to an oral extract composition comprising Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.) extract composition, and its process of preparation. Also relates to the oral extract composition formulation comprising with synergistic combinations of the Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.) in suitable doses and at least one nutraceutically and / or pharmaceutically accepted excipient for, improvement, and maintenance of women health more preferably in improving of hot flashes, night sweats, poor sleep, anxiety, sexual dysfunction, mood changes, Genitourinary symptoms in overall improving the quality of life, serum estradiol, progesterone, follicle stimulating hormone (FSH), and cortisol during the period of menopause (perimenopause and menopause). The oral extract composition formulation comprising with synergistic combinations of the Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.) in suitable doses and at least one nutraceutically and / or pharmaceutically accepted excipient is used for improving Brain-derived neurotrophic factor (BDNF) levels in women during the period of menopause. The present invention, further related to the extraction process of Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.), also provides the process for the preparation of formulation composition. This invention further relates to the extraction process from Shatavari (Asparagus racemosus Willd.) and Fenugreek (Trigonella foenum-graecum L.) either as individual herbs or co-extraction of the two herbs or mixing the extracts at various stages of the individual extracts.

The invention discloses an aptamer electrochemical biosensor for estradiol detection as well as a preparation method and application of the aptamer electrochemical biosensor. The aptamer electrochemical biosensor is obtained by growing AuNPs particles on the surface of MoS2 to prepare a MoS2-AuNPs composite material, fixing the composite material on the surface of an electrode through chitosan, hybridizing aptamer probes P1 and P2, and self-assembling the aptamer probes P1 and P2 on the surface of the electrode through Au-S bonds. The sensor can be used for in-situ detection of hormone factor estradiol in sweat, can be applied to non-invasive monitoring equipment, and provides reliable and timely monitoring data.

The invention discloses bifidobacterium adolescentis for relieving estrogen-related metabolic disorder and obesity and application thereof, and belongs to the technical field of microorganisms. The bifidobacterium adolescentis CCFM1506 screened by the invention can effectively improve ovariectomized individual estrogen disorder symptoms, obviously slow down the weight increase speed and weight increment of estrogen disorder individuals, increase the expression quantity of serum estradiol, CYP11A1 and ERalpha in adrenal gland and the high-density lipoprotein content in serum, reduce the total cholesterol and glucose level and improve the ovariectomized ovariectomized ovariectomized ovariectomized ovariectomized ovariectomized ovariectomized ovariectomized ovariectomized abnormal glucose and lipid metabolism is adjusted; the invention has the advantages of simple preparation method and wide application prospect, can be used for preparing medicines for relieving or treating estrogen-related metabolic disorders and obesity, and can be used for preparing health-care products beneficial to controlling in-vivo fat and maintaining the health level of blood fat, and has obvious physiological effects and wide application prospects, and can be used for preparing the medicines for relieving or treating the estrogen-related metabolic disorders and obesity and preparing health-care products beneficial to controlling in-vivo fat and maintaining the health level of blood fat. A new thought and a new basis are provided for developing functional foods or medicines based on the probiotics.

Provided are methods of contraception using as a first line combined oral contraceptive (COC), a COC composition comprising nomegestrol acetate (NOMAC) and estradiol or an ester thereof. Said COC compositions are associated with a lower risk of venous thromboembolism (VTE) as compared to other CHCs. The compositions may also be used in methods of treatment in women having conditions linked to menstruation and / or fertility.

This invention discloses a method for large-scale breeding of all-male salmon and trout, belonging to the field of fish sex regulation breeding technology. The method includes: collecting fertilized eggs from different families of the same species of salmon and trout, artificially incubating them to obtain fry, and inducing sex reversal in the fry by using 17β-estradiol when they begin feeding; subsequently, raising the fry to sexual maturity, selecting individuals with a female phenotype, and then using sdY as a sex identification gene, selecting pseudo-females with genotype XY by quantitative real-time PCR; pairing the sexually mature pseudo-females with genotype XY with normal males with genotype XY, and obtaining super-males with genotype YY through artificial insemination; pairing the sexually mature super-males with females with genotype XX, and producing all-male salmon and trout with genotype XY on a large scale through artificial insemination. This invention overcomes the problem that existing technologies cannot achieve large-scale breeding of all-male salmon and trout.

According to the synthesis method, methylbenzene is used as a solvent, benzoic anhydride is used as an acylation reagent, and on the premise that the synthesis efficiency and quality are guaranteed, the defects that in the prior art, when a reagent is used, safety risks exist, and the cost is high are effectively overcome. In the production process, special storage and operation conditions are not needed, the product quality is controllable, and the method is suitable for industrial production.

This invention belongs to the field of analytical detection technology and relates to a method for detecting estradiol using a combination of organometallic framework enrichment and enzyme-linked immunosorbent assay (ELISA). A magnetic organometallic framework solid-phase extractant is used to enrich estradiol in the sample, followed by detection of the enriched estradiol using ELISA. The magnetic organometallic framework solid-phase extractant is prepared by subjecting mercaptoacetic acid-modified Fe3O4 particles, zirconium salt, and 2-amino-terephthalic acid to a solvothermal reaction at 110–130°C for 22–26 hours. The detection method provided by this invention has advantages such as short detection time, high sensitivity, good specificity, and high accuracy, thereby enabling the detection of trace amounts of estradiol in environmental water samples.

An Artificial Intelligence (AI) system trained by a plurality of training data. The AI system is trained by relationships between a time to final menstrual period and ratios between follicle-stimulating hormone levels and estradiol levels, anti-mullerian hormone, race, cholesterol levels, and a presence of one or more contraceptives. The AI system is configured to accept data from only one sample from a user as input and generate a prediction of a time to final menstrual period for the user as output.

The process comprises combining prepared β-cyclodextrin solution and prepared ethinyl estradiol solution, then removing the solvent by spray-drying for obtaining an ethinyl estradiol β-cyclodextrin complex. The obtainable amorphous ethinyl estradiol β-cyclodextrin complex is suitable for use in pharmaceutical compositions and formulations comprising it.

This invention discloses an in vitro maturation culture medium for ferret oocytes and its preparation method, belonging to the field of animal embryo engineering technology. It comprises the following components: TCM199 basal culture medium, 50 mL fetal bovine serum, 25 IU follicle-stimulating hormone, 25 IU luteinizing hormone, 0.5 mg 17β-estradiol, 0.005 mg epidermal growth factor, 12.1 mg sodium pyruvate, 0.2 mM cysteine, 0.1 mM cysteine, 0.030 g penicillin, 0.025 g streptomycin sulfate, as well as an antioxidant complex, a combination of growth factors, and extracellular matrix components. This invention provides a comprehensive and suitable nutritional environment for ferret oocytes by precisely formulating a composite culture medium containing multiple key components. Specifically, the hormone combination at specific concentrations accurately simulates the in vivo physiological environment, effectively regulating the oocyte maturation process; multiple growth factors work synergistically to promote oocyte growth, development, and differentiation; and the addition of the antioxidant complex effectively eliminates free radicals generated during in vitro culture, reducing oxidative stress damage to oocytes.

The invention belongs to the technical field of transdermal patches, and relates to a transdermal patch for improving the bioavailability of estradiol and a preparation method of the transdermal patch. The transdermal patch is prepared from the following components in percentage by weight: 5 to 21 percent of estradiol, 8 to 15 percent of polymer matrix, 0.5 to 1.5 percent of butylated hydroxytoluene, 10 to 15 percent of hydrogenated rosin glyceride, 2 to 5 percent of penetration enhancer, 3 to 8 percent of plasticizer and 25 to 35 percent of solvent. According to the transdermal patch disclosed by the invention, continuous and stable release of a medicine is realized, the transdermal absorption rate of estradiol is improved, so that the bioavailability is improved, the treatment effect is further improved, and meanwhile, the transdermal patch disclosed by the invention can also avoid a first-pass effect of the liver and reduce side effects; the transdermal drug delivery system is convenient to use, and the compliance of a patient is improved.

The invention belongs to the technical field of medicines, and relates to an estradiol valerate film-coated tablet and a preparation method thereof. The estradiol valerate sugar film-coated tablet comprises the following components in percentage by mass: 60%-80% of an estradiol valerate tablet core and 20%-40% of a water-based sugar film coating, the water-based sugar film coating comprises a powder coating layer and a coloring layer. The preparation method of the estradiol valerate film-coated tablet comprises the following steps: preheating the estradiol valerate tablet core; coating with a powder coating layer; drying the coated tablet of the estradiol valerate powder coating layer; coating a coloring layer; and drying and cooling the estradiol valerate coloring layer coated tablet to obtain the estradiol valerate sugar film coated tablet. According to the estradiol valerate sugar film-coated tablet and the preparation method thereof provided by the invention, the coating process is simple and efficient, and the stability of related substances of a preparation can be ensured while the process is optimized.

The application provides an application of a traditional Chinese medicine preparation in preparation of a drug for treating dysmenorrhea, and an active ingredient of the traditional Chinese medicine preparation is prepared from liquorice, asarum, cassia twig, angelica, white peony root, common sage and chuanxiong. The traditional Chinese medicine preparation provided by the application fuses multi-dimensional treatment strategies of warming meridians, nourishing blood, regulating qi and dredging collaterals, and can cover different TCM syndrome types such as cold coagulation and blood stasis, qi stagnation and blood stasis and qi and blood deficiency. Pharmacological experiments show that for dysmenorrhea rats of the cold coagulation and blood stasis type, the traditional Chinese medicine preparation of the application can reduce the sensitivity of the uterus caused by benzoic acid estradiol and inhibit the writhing reaction of the rats caused by oxytocin, which indicates that the traditional Chinese medicine preparation of the application has the effect of treating dysmenorrhea.

Among hospital–acquired infections, Pseudomonas aeruginosa-associated urinary tract infections (UTIs) are mainly caused by indwelling urethral catheters (catheter-associated UTIs or CAUTIs) and are difficult to treat, resulting in high rates of morbidity among hospitalized patients. While antibiotics can successfully treat bacteria in the bladder lumen, they are inefficient at crossing stratified urothelium plasma membranes to kill persistent intracellular bacterial communities (IBCs). Herein, UTI IBCs are targeted by locally delivering the antibiotic gentamicin and / or the steroid hormone estradiol via polymeric nanogels conjugated with a cell-penetrating peptide Cys-Gly-Lys-Arg-Lys (CGKRK - SEQ ID NO:1). This approach delivered more intracellular gentamicin compared to drug delivered in solution in vitro and more estradiol compared to conventional methods. In an acute UTI murine model, the nanogel cell-penetrating peptide drug delivery system facilitated the transport of gentamicin into the urothelium and resulted in > 90% clearance of a uropathogenic P. aeruginosa clinical strain in vivo.

The application relates to the field of biotechnology, and particularly relates to an application of Bacteroides ovatus in ovarian aging; the application comprises: taking low-abundance Bacteroides ovatus as a biomarker of ovarian aging; the application comprises using Bacteroides ovatus in a medicine for delaying ovarian aging; the application further comprises using a fermentation product of Bacteroides ovatus in the medicine for delaying ovarian aging; it is found through experiments that there is a significant difference in Bacteroides ovatus in mice with different degrees of ovarian aging, and it is found through subsequent experiments that Bacteroides ovatus has the effects of significantly increasing the number of oocytes, up-regulating anti-Mullerian hormone, up-regulating the level of estradiol and down-regulating the level of follicle-stimulating hormone, which indicates that Bacteroides ovatus can be used in the medicine for delaying ovarian aging.