89 results about "Steroidal hormones" patented technology

A nanoscale molecularly imprinted polymer (MIP) thin film for small molecule detection, a method of manufacturing the same, and an electrochemical sensor using the MIP and quantum electrochemical impedance spectroscopy (EIS) are described. A plurality of specific recognition spaces for small molecules of 1000 Da or less are formed in the MIP, a receptor polymer is present at one end of the specific recognition spaces, a redox probe is present in a wire form, and the small molecule is a steroid hormone or a protein. The sensor is useful in point-of-care applications because it exhibits a rapid and reversible small molecule detection ability through a simple electrochemical regeneration process without cumbersome washing and solution replacement steps in the manufacturing process, thereby enabling continuous detection.

Probes that are versatile, easy to use, and provide rapid results for detecting and quantifying levels of small molecules that include steroids, hormones, antibodies, aptamers and enzymes such as various steroidal hormones like estrogen, progesterone and testosterone in samples. This is particularly useful in home and clinical settings. A probe useful in competitive assays includes a competitive ligand bound to a linker molecule bound to a detectable tag. The linker may be chemical, DNA or a combination of both

The invention discloses a steroid hormone degrading bacterium as well as a screening method and application thereof, and relates to the field of biological medicines, the steroid hormone degrading bacterium comprises klebsiella pneumoniae (KP2022 Klebsiella pneumoniae) with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M2025478. The steroid hormone degrading bacterium provided by the invention has the capability of efficiently degrading dexamethasone, is beneficial to degrading dexamethasone in the environment, is conveniently obtained by adopting a screening method, and provides a sample for research on degradation of steroid hormones.

The present invention relates to a method for detecting steroid hormones in serum, comprising the following steps: (1) adding 0.1 to 20 mg of a magnetic solid phase extractant to each milliliter of a sample to be tested, mixing, magnetically separating, and removing liquid; adding a washing solution, mixing, magnetically separating, and removing liquid; adding an eluent for elution, mixing, magnetically separating, and collecting the eluent to obtain a test solution; (2) detecting the hormone content in the test solution by liquid chromatography and tandem mass spectrometry; wherein the hormone is selected from any one of testosterone, androstenedione, dihydrotestosterone, dehydroepiandrosterone sulfate, and 17α-hydroxyprogesterone or a combination thereof; the magnetic solid phase extractant comprises a magnetic core ferrosoferric oxide, a middle layer structure silica wrapping the magnetic core, and an outer layer mesoporous structure, wherein the outer layer mesoporous structure is selected from any one of polystyrene and polystyrene divinylbenzene or a combination thereof. The present invention has the characteristics of good stability, high accuracy and sensitivity, strong specificity, high recovery rate, simple and controllable operation, fast analysis speed, etc., and can be used for simultaneous quantitative detection of multiple hormones in clinical practice.

The invention relates to a preparation method and application of a releasing agent, and belongs to the technical field of steroid hormone releasing agents. The releasing agent is prepared from the following components in percentage by mass: 0.2 to 1 percent of an amide compound, 0.02 to 0.06 percent of a metal chelate, 0.05 to 0.15 percent of a preservative, 0.2 to 0.5 percent of a surfactant, 0.015 to 0.045 percent of a steroid heterocyclic compound and the balance of deionized water, wherein the amide compound is a composition of sodium alpha-mercaptoethanesulfonate and N-methylacetamide, and the amide compound is a compound of sodium alpha-mercaptoethanesulfonate and N-methylacetamide; the steroid heterocyclic compound is a composition of methyltestosterone, danazol and stanolone. According to the universal releasing agent, the four hormones in a sample can be effectively released from respective binding proteins to be in a free state, and clinical test data of the sample can be obtained by measuring the content of the free hormones, so that the measured test result is more accurate.

Among hospital–acquired infections, Pseudomonas aeruginosa-associated urinary tract infections (UTIs) are mainly caused by indwelling urethral catheters (catheter-associated UTIs or CAUTIs) and are difficult to treat, resulting in high rates of morbidity among hospitalized patients. While antibiotics can successfully treat bacteria in the bladder lumen, they are inefficient at crossing stratified urothelium plasma membranes to kill persistent intracellular bacterial communities (IBCs). Herein, UTI IBCs are targeted by locally delivering the antibiotic gentamicin and / or the steroid hormone estradiol via polymeric nanogels conjugated with a cell-penetrating peptide Cys-Gly-Lys-Arg-Lys (CGKRK - SEQ ID NO:1). This approach delivered more intracellular gentamicin compared to drug delivered in solution in vitro and more estradiol compared to conventional methods. In an acute UTI murine model, the nanogel cell-penetrating peptide drug delivery system facilitated the transport of gentamicin into the urothelium and resulted in > 90% clearance of a uropathogenic P. aeruginosa clinical strain in vivo.

The invention relates to a method for simultaneously detecting various steroid hormones in serum, which comprises the following steps: extracting hormones in serum by a liquid-liquid extraction method; a derivatization reagent is added into the extracted hormone, an oximation reaction is carried out, and the derivatization reagent comprises quaternary ammonium oxygen amine; the hormone after oximation reaction is injected into a two-dimensional liquid chromatography-tandem mass spectrometry system for detection, the hormone is enriched on a one-dimensional chromatographic column, and then the enriched hormone is subjected to back flushing and transferred to a two-dimensional chromatographic column for analysis. According to the method, the backwashing transfer two-dimensional liquid chromatography-tandem mass spectrometry and the derivatization reaction are combined, various steroid hormones including progestational hormone and androgen can be detected at the same time, and the method has the advantage of being high in detection sensitivity.

The present application relates to a compound represented by formula (I), and the compound can inhibit steroid hormone-dependent disorders and diseases of CYP11A1. The present application further comprises a pharmaceutical composition comprising the compound represented by formula (I) and a method for treating a condition related to CYP11A1 activity.

The invention provides a method and a kit for determining various steroid hormones in body fluid by liquid chromatography-mass spectrometry. The method comprises the following steps: pre-treating a biological fluid sample by introducing an isotope internal standard, a salting-out auxiliary reagent, an extracting agent and an automatic pipetting platform, then separating an isomeride and an interferent through liquid chromatography, switching quantitative ions and qualitative ions with positive-negative scanning characteristics by tandem mass spectrometry, making a standard curve by using a calibrator for quantification, and finally determining the content of the biological fluid sample. And low, medium and high quality control products continuously monitor the performance of the method. According to the method, the aim of simultaneously analyzing and detecting 28 steroid hormones and 24 D / 13C / 15N labeled isotope hormones in a sample by using a UPLC-MS / MS technology without using solid-phase extraction and non-derivatization treatment is achieved, and the method is rapid, simple and convenient to operate, multiple in detection variety, high in flux, high in specificity, low in cost, high in sensitivity and high in sensitivity. 28 indexes of a steroid hormone metabolic pathway are effectively and systematically monitored.

The present invention discloses a method for simultaneously detecting a plurality of fat-soluble vitamins and steroid hormones, comprising the following steps: adding an internal standard to a serum sample, mixing, and then adding methyl tert-butyl ether for extraction; extracting the components to be tested into an organic solvent, centrifuging, drying the extract with nitrogen, re-dissolving, and then injecting and analyzing the extract on a liquid chromatography-mass spectrometry system. The detection method of the present invention can simultaneously detect the contents of five fat-soluble vitamins and seventeen steroid hormones in serum (plasma), and simultaneously test a total of 22 substances to be tested, which is accurate and can be quantitatively detected. The present invention adopts a scheme that changes the conventional prior art of using the isotope of the substance to be tested as the internal standard substance, and adopts trenbolone cyclohexylmethyl carbonate as the internal standard, which can simultaneously quantify 22 substances to be tested, greatly reducing the workload.

The present disclosure relates to a method for detecting vitamin D or a steroid hormone and a blood sample pretreatment kit. The method for detecting vitamin D in blood comprises pretreating a blood sample to obtain a test sample, and detecting the test sample by liquid chromatography tandem mass spectrometry, wherein the pretreatment comprises: adding an internal standard solution and a magnetic bead reagent to the blood sample to obtain a mixed solution and incubating to allow the target analyte to bind to the surface of the magnetic beads; separating the magnetic beads combined with the target analyte; and eluting the target analyte from the surface of the magnetic beads to obtain the test sample, wherein the mixed solution comprises methanol in an amount of 45vol% to 60vol%, or ethanol in an amount of 30vol% to 50vol%. The method can significantly reduce non-specific adsorption of magnetic beads, and improve the accuracy and sensitivity of detection.

The invention relates to the technical field of in-vitro diagnostic reagents, in particular to a steroid hormone-containing immunosuppressive agent precise monitoring treatment kit, and discloses a joint detection and correction method for precise monitoring of a steroid hormone-containing immunosuppressive agent. Comprising the following steps: step 1, collecting clinical samples to be detected, and subpackaging each collected sample into two equal parts; 2, carrying out immunological quantitative determination on the first equal part of sample by adopting an immunological kit to obtain an immunological determination value; 3, the second equal sample corresponding to the step 2 is subjected to reference quantitative determination through liquid chromatography-tandem mass spectrometry, a mass spectrometry determination value is obtained, and mass spectrometry quantification uses an isotope labeling internal standard corresponding to each target analyte and is carried out according to a standardized sample pretreatment process. According to the method, high throughput and operation convenience of the immunoassay are guaranteed, meanwhile, the accuracy equivalent to that of mass spectrometry is provided, and a reliable basis is provided for clinical individualized medication.

The present disclosure provides novel compositions for biologically active compounds that are administered through injection. The active compounds include, but are not limited to steroid hormones, diarylheptanoids, and flavonoids. These compositions are distinguished from the prior art through the addition of an oil to the overall composition, which results in a biphasic formulation. The oil serves to enhance the efficiency of delivery of the formulation to a human or animal subject, and reduces discomfort associated with the injection process. Optionally, one or more additional substances, each of which is soluble in oil, can be added to the oil to enable the deliverable of multiple active compounds in a single injection.

The present invention addresses the problem of providing: a steroid hormone detection method and steroid hormone detection kit that are capable of avoiding the influence of steroid hormone-binding proteins during the measurement of steroid hormones in a biological sample; a compound and inclusion complex that can be used in the abovementioned detection method and detection kit; and a composition containing the abovementioned compound or inclusion complex. The present invention provides a steroid hormone detection method involving: bringing a hydrogen halide salt or cyclodextrin inclusion complex of a compound represented by formula [1] into contact with a complex of a steroid hormone-binding protein and steroid hormones in a biological sample; and detecting steroid hormones. In the formula, R1, R2, and R3 are each independently a hydrogen atom or 1-3C alkyl group. R4 represents a halogen atom, a 1-3C alkyl group, a phenyl group, a methoxy group, or an ethoxy group.

The invention discloses a rapid detection method for 27 steroid hormones in mangrove plants. The method comprises the following steps: S1, putting a mangrove plant sample subjected to freeze drying, grinding and sieving into a centrifugal tube A, and then adding an isotope internal standard for balancing; adding acetonitrile, carrying out vortex, ultrasonic extraction and centrifugation, and taking supernate; s2, methanol and acetonitrile are sequentially added, vortex, ultrasonic extraction and centrifugation are performed, and supernate obtained after acetonitrile extraction in the S1, supernate obtained after methanol extraction in the S2 and supernate obtained after acetonitrile extraction in the S2 are combined; s3, transferring the supernate into a centrifugal tube B containing filler, carrying out vortex centrifugation, and putting the supernate into a glass test tube; s4, extracting the filler in S3 again by using acetonitrile, carrying out vortex centrifugation, and combining the two supernatants in a glass test tube; s5, carrying out nitrogen blowing concentration on the solution in the glass test tube, and fixing the volume in methanol; s6, determining the steroid hormones and the metabolites thereof by using high performance liquid chromatography-tandem mass spectrometry, and obtaining the content level of the steroid hormones and the metabolites thereof in the plants through data analysis and quantitative calculation.

The invention relates to the technical field of livestock breeding biology, and discloses a method for promoting porcine ovary granular cell proliferation, which comprises the following steps: adding a porcine follicular fluid exosome into a porcine ovary granular cell culture system, and acting for a certain time at an effective concentration, so as to improve the proliferation activity of granular cells. After the exosome treatment, the apoptosis rate of granular cells can be reduced, the cell survival rate can be improved, and the granular cells can be promoted to secrete steroid hormones, including the estradiol level and the expression of progesterone synthetase. Cell experiments prove that the follicular fluid exosome can effectively improve the activity of granular cells, promote the process of a cell cycle, inhibit cell apoptosis and remarkably enhance the synthesis capacity of steroid hormones such as estradiol and progesterone, and a new intervention strategy is provided for improving follicular development and reproductive performance of sows.

The invention particularly relates to a steroid hormone magnetic particle mass spectrometry detection kit and a detection method thereof. The kit comprises a reagent 0, a reagent 1, a reagent 2, a reagent 3, a reagent 4, a calibration product and a quality control product, the reagent 0 is a magnetically attractable steroid hormone reagent 0 working solution, the reagent 1 is a steroid hormone isotope internal standard reagent, the reagent 2 and the reagent 3 are elution reagents, and the reagent 4 is an elution reagent. By sequentially carrying out adsorption, leaching and elution treatment, one-time magnetic solid-phase extraction of steroid hormones in a biological sample is realized, and a supernatant for liquid chromatography-tandem mass spectrometry detection is obtained. According to the detection method, synchronous detection of multiple steroid hormones is realized in combination with a liquid chromatography-tandem mass spectrometry multi-reaction monitoring mode under a non-derivatization condition. According to the technical scheme, the sample pretreatment process is simplified, the detection specificity and stability are improved, and the method is suitable for multi-index quantitative detection of steroid hormones.

The invention relates to a preparation method of steroid hormone blank matrix serum. The invention particularly relates to a preparation method of steroid hormone blank matrix serum. The preparation method comprises the following steps: activating HLB (Hydrophile Lipophile Balance) magnetic beads by using an ammonium acetate methanol solution; the method comprises the following steps: mixing serum with activated HLB magnetic beads, and extracting steroid hormones in the serum; and applying a magnetic field to adsorb the HLB magnetic beads, and collecting a first supernatant. The invention also relates to a method for extracting the steroid hormone in the serum and a method for regenerating the HLB magnetic beads for extracting the steroid hormone. The method is simple, rapid and safe to operate; the requirements of automatic equipment can be met, and the method is particularly suitable for automatic large-scale preparation; meanwhile, the prepared blank matrix serum is suitable for being used as a blank control or a blank diluent in detection.

The invention discloses a method for evaluating the influence of human interference on reproductive health of wild animals. The method comprises the following steps: acquiring steroid hormone species and intestinal microorganism metagenome assembly genomes (MAGs) of a sentry animal excrement sample; based on the steroid hormone type, a first gene set is determined, the first gene is a metabolic enzyme gene, and metabolic enzyme participates in steroid hormone metabolism; determining a first set of coding sequences (CDSs) based on the first set of genes and the MAGs, the first CDS being from the MAGs and a coding region of the first gene comprising the first CDS; determining a first set of microorganisms (HCBs) based on the first set of CDSs, the HCBs being a set of host bacteria (HCB) of the first CDSs, the HCBs being selected from the sentry animal gut microbiota; and based on the total relative abundance of the HCBs, determining whether the human interference affects the reproductive health of the wild animals. The method is scientific and reliable, and provides a scientific decision basis for wild animal protection.

The invention discloses application of cinnamyl aldehyde, and cinnamyl aldehyde can regulate synthesis and secretion of androgen testosterone and can be applied to related products for regulating synthesis and secretion of androgen testosterone. In addition, tests prove that cinnamyl aldehyde increases synthesis and secretion of testicular interstitial cell androgen by improving activity of a steroid hormone synthesis nuclear receptor promoter and up-regulating expression of steroid hormone synthetase.

The application discloses a method for evaluating the influence of human interference on the reproductive health of wild animals. A steroid hormone species and an intestinal microorganism macro-genome assembly genome MAG of a sentinel animal fecal sample are obtained; based on the steroid hormone species, a first gene set is determined, the first gene set comprising a first gene, the first gene being a metabolic enzyme gene, the metabolic enzyme participating in steroid hormone metabolism; based on the first gene set and the MAGs, a first coding sequence CDS set is determined, the first coding sequence CDS set comprising a first CDS, the first CDS being from the MAGs, and the coding region of the first gene comprising the first CDS; based on the first coding sequence CDS set, a first microorganism set HCBs is determined, the HCBs being a collection of host bacteria HCBs of the first CDS, the HCBs being selected from the intestinal flora of the sentinel animal; based on the total relative abundance of the HCBs, it is determined whether human interference affects the reproductive health of wild animals. The method is scientific and reliable, and provides a scientific decision basis for wild animal protection.

The invention relates to the technical field of analysis and detection, in particular to a detection method for quantitative urine steroid hormone based on efficient enzymolysis. The invention establishes a detection method for accurately quantifying 35 steroid hormones in urine by combining a high performance beta glucuronide enzymatic dissociation method with high performance liquid chromatography-tandem mass spectrometry. According to the method, the pretreatment time required by clinical determination of urine steroid hormones can be remarkably shortened, single-needle quantitative detection of 35 steroid hormones is realized, and the method has a wide application prospect.

Probes that are versatile, easy to use, and provide rapid results for detecting and quantifying levels of small molecules that include steroids, hormones, antibodies, aptamers and enzymes such as various steroidal hormones like estrogen, progesterone and testosterone in samples. This is particularly useful in home and clinical settings. A probe useful in competitive assays includes a competitive ligand bound to a linker molecule bound to a detectable tag. The linker may be chemical, DNA or a combination of both.

The invention provides a mass spectrum detection system and a mass spectrum detection method for on-site rapid quantification of steroid hormones. The detection system comprises: a pretreatment module for pretreating a biological sample to obtain a first sample; the derivatization module is used for carrying out derivatization treatment on the first sample to obtain a second sample; and the capillary electrophoresis-mass spectrometry analysis module is used for sequentially carrying out electrophoretic separation and mass spectrometry detection on the second sample and carrying out qualitative and quantitative analysis on the steroid hormone in the second sample. Therefore, by adopting the detection system disclosed by the invention, the ionization efficiency and the analysis sensitivity of the steroid hormone can be improved. The capillary electrophoresis-mass spectrometry module is adopted, so that the whole analysis process is efficient and rapid, the analysis time is effectively controlled within 10 minutes, and the requirement of instant field detection can be fully met due to the high efficiency.

The invention relates to an application of an HK1 gene in mouse ovarian granular cells, in particular to an application in regulating proliferation and apoptosis of the mouse ovarian granular cells and / or steroid hormone synthesis. According to the invention, mouse ovarian granular cells are taken as a research object, and the expression of the HK1 gene is interfered to verify that the HK1 gene is taken as a key target spot for regulating and controlling the functions of the mouse ovarian granular cells so as to influence steroid hormone synthesis and cell proliferation and apoptosis processes. The invention not only provides more reliable technical support for ovarian function regulation, but also provides new thinking and technical guidance for mechanism research of reproductive diseases and preparation and development of related drugs.

The present invention discloses a strain of Bacillus marinus and its application. The Bacillus marinus is named strain HN14, and its taxonomic name is Pontibacillus chungwhensis. It is preserved in the Guangdong Microbial Culture Collection Center, and its preservation number is GDMCC No. 62925. The strain HN14 has the ability to degrade a variety of organic pollutants, including steroid hormones (estradiol, testosterone) and polycyclic aromatic hydrocarbons (phenanthrene, pyrene, benzo[a]pyrene). The strain HN14 can still maintain efficient degradation of estradiol at a salinity of 25%, providing a new strain option for the remediation of high-salinity environmental pollution.

The present invention relates to the field of biological detection, and in particular to a kit and method for simultaneously determining multiple steroid hormones in serum. The present invention provides a detection reagent, including: a calibrator, a quality control product, an internal standard, an extractant, a resolvent and a buffer; the extractant includes one or more of methyl tert-butyl ether, ethyl acetate, n-hexane, cyclohexane or n-heptane; the resolvent includes a methanol aqueous solution. The kit provided by the present invention only requires 200 μL of serum sample to achieve the purpose of simultaneously detecting the concentrations of multiple steroid hormones, and only uses a two-step extraction method to accurately determine the content of multiple steroid hormones at extremely low concentrations (ng / mL level) in blood. Compared with the existing technology for detecting multiple steroid hormones, the kit provided by the present invention is simple to operate, sensitive and accurate, has a wide linear dynamic range (400 times), can simultaneously determine multiple steroid hormones, and can be used for routine detection of multiple steroid hormones in clinical serum.

The invention relates to the technical field of biomedical analysis, in particular to a clinical steroid hormone determination method which comprises the following steps: repeatedly treating human plasma by adopting a solid-phase extraction method, using octadecylsilane bonded silica gel filler, extracting to remove endogenous steroid hormone, and obtaining a human plasma blank matrix; the method comprises the following steps: respectively preparing a steroid hormone stock solution and a loratadine internal standard solution, taking the human plasma blank matrix treated in the step 1, adding the loratadine internal standard solution, treating with a 2% formic acid solution, extracting by using an HLB solid-phase extraction column, centrifugally concentrating an eluent, redissolving for LC-MS / MS analysis, carrying out sample analysis by adopting an LC-MS / MS method, and determining the content of the loratadine in the human plasma blank matrix. And detecting in an ESI positive ionization mode. Therefore, multiple steroid hormones can be detected at the same time, the coverage concentration range is wide, endogenous interference can be effectively removed, the detection accuracy is improved, the sensitivity is high, the repeatability is good, and the method is suitable for high-throughput analysis of clinical samples.

The invention provides a kit for simultaneously detecting six steroid hormones and application of the kit. The steroid hormones comprise testosterone, androstenedione, dehydroepiandrosterone, dehydroepiandrosterone sulfate, dihydrotestosterone and 17alpha-hydroxyprogesterone. The kit comprises extract liquor, a mobile phase additive and a standard quality control product. The kit provided by the invention can be used for simultaneously detecting testosterone, androstenedione, dehydroepiandrosterone, dehydroepiandrosterone sulfate, dihydrotestosterone and 17alpha-hydroxyprogesterone, and has the advantages of high accuracy, low quantitation limit and wide detection range.

The invention discloses application of silibinin in preparation of a medicine for treating autoimmune hepatitis, and belongs to the field of biological medicine application. The silybin has a good liver protection effect, and a pathological experiment combined with a related mouse disease model in development of autoimmune liver injury pathogenesis research finds that the silybin can well relieve inflammation caused by the autoimmune liver injury, so that the silybin can replace or assist a traditional steroid hormone therapy, and the silybin has a good application prospect. And side effects caused by traditional therapy are reduced.