33 results about "Steroidal hormones" patented technology

Among hospital–acquired infections, Pseudomonas aeruginosa-associated urinary tract infections (UTIs) are mainly caused by indwelling urethral catheters (catheter-associated UTIs or CAUTIs) and are difficult to treat, resulting in high rates of morbidity among hospitalized patients. While antibiotics can successfully treat bacteria in the bladder lumen, they are inefficient at crossing stratified urothelium plasma membranes to kill persistent intracellular bacterial communities (IBCs). Herein, UTI IBCs are targeted by locally delivering the antibiotic gentamicin and / or the steroid hormone estradiol via polymeric nanogels conjugated with a cell-penetrating peptide Cys-Gly-Lys-Arg-Lys (CGKRK - SEQ ID NO:1). This approach delivered more intracellular gentamicin compared to drug delivered in solution in vitro and more estradiol compared to conventional methods. In an acute UTI murine model, the nanogel cell-penetrating peptide drug delivery system facilitated the transport of gentamicin into the urothelium and resulted in > 90% clearance of a uropathogenic P. aeruginosa clinical strain in vivo.

The invention relates to a method for simultaneously detecting various steroid hormones in serum, which comprises the following steps: extracting hormones in serum by a liquid-liquid extraction method; a derivatization reagent is added into the extracted hormone, an oximation reaction is carried out, and the derivatization reagent comprises quaternary ammonium oxygen amine; the hormone after oximation reaction is injected into a two-dimensional liquid chromatography-tandem mass spectrometry system for detection, the hormone is enriched on a one-dimensional chromatographic column, and then the enriched hormone is subjected to back flushing and transferred to a two-dimensional chromatographic column for analysis. According to the method, the backwashing transfer two-dimensional liquid chromatography-tandem mass spectrometry and the derivatization reaction are combined, various steroid hormones including progestational hormone and androgen can be detected at the same time, and the method has the advantage of being high in detection sensitivity.

The present application relates to a compound represented by formula (I), and the compound can inhibit steroid hormone-dependent disorders and diseases of CYP11A1. The present application further comprises a pharmaceutical composition comprising the compound represented by formula (I) and a method for treating a condition related to CYP11A1 activity.

The present disclosure relates to a method for detecting vitamin D or a steroid hormone and a blood sample pretreatment kit. The method for detecting vitamin D in blood comprises pretreating a blood sample to obtain a test sample, and detecting the test sample by liquid chromatography tandem mass spectrometry, wherein the pretreatment comprises: adding an internal standard solution and a magnetic bead reagent to the blood sample to obtain a mixed solution and incubating to allow the target analyte to bind to the surface of the magnetic beads; separating the magnetic beads combined with the target analyte; and eluting the target analyte from the surface of the magnetic beads to obtain the test sample, wherein the mixed solution comprises methanol in an amount of 45vol% to 60vol%, or ethanol in an amount of 30vol% to 50vol%. The method can significantly reduce non-specific adsorption of magnetic beads, and improve the accuracy and sensitivity of detection.

The invention relates to the technical field of in-vitro diagnostic reagents, in particular to a steroid hormone-containing immunosuppressive agent precise monitoring treatment kit, and discloses a joint detection and correction method for precise monitoring of a steroid hormone-containing immunosuppressive agent. Comprising the following steps: step 1, collecting clinical samples to be detected, and subpackaging each collected sample into two equal parts; 2, carrying out immunological quantitative determination on the first equal part of sample by adopting an immunological kit to obtain an immunological determination value; 3, the second equal sample corresponding to the step 2 is subjected to reference quantitative determination through liquid chromatography-tandem mass spectrometry, a mass spectrometry determination value is obtained, and mass spectrometry quantification uses an isotope labeling internal standard corresponding to each target analyte and is carried out according to a standardized sample pretreatment process. According to the method, high throughput and operation convenience of the immunoassay are guaranteed, meanwhile, the accuracy equivalent to that of mass spectrometry is provided, and a reliable basis is provided for clinical individualized medication.

The present disclosure provides novel compositions for biologically active compounds that are administered through injection. The active compounds include, but are not limited to steroid hormones, diarylheptanoids, and flavonoids. These compositions are distinguished from the prior art through the addition of an oil to the overall composition, which results in a biphasic formulation. The oil serves to enhance the efficiency of delivery of the formulation to a human or animal subject, and reduces discomfort associated with the injection process. Optionally, one or more additional substances, each of which is soluble in oil, can be added to the oil to enable the deliverable of multiple active compounds in a single injection.

The invention relates to the technical field of livestock breeding biology, and discloses a method for promoting porcine ovary granular cell proliferation, which comprises the following steps: adding a porcine follicular fluid exosome into a porcine ovary granular cell culture system, and acting for a certain time at an effective concentration, so as to improve the proliferation activity of granular cells. After the exosome treatment, the apoptosis rate of granular cells can be reduced, the cell survival rate can be improved, and the granular cells can be promoted to secrete steroid hormones, including the estradiol level and the expression of progesterone synthetase. Cell experiments prove that the follicular fluid exosome can effectively improve the activity of granular cells, promote the process of a cell cycle, inhibit cell apoptosis and remarkably enhance the synthesis capacity of steroid hormones such as estradiol and progesterone, and a new intervention strategy is provided for improving follicular development and reproductive performance of sows.

The invention particularly relates to a steroid hormone magnetic particle mass spectrometry detection kit and a detection method thereof. The kit comprises a reagent 0, a reagent 1, a reagent 2, a reagent 3, a reagent 4, a calibration product and a quality control product, the reagent 0 is a magnetically attractable steroid hormone reagent 0 working solution, the reagent 1 is a steroid hormone isotope internal standard reagent, the reagent 2 and the reagent 3 are elution reagents, and the reagent 4 is an elution reagent. By sequentially carrying out adsorption, leaching and elution treatment, one-time magnetic solid-phase extraction of steroid hormones in a biological sample is realized, and a supernatant for liquid chromatography-tandem mass spectrometry detection is obtained. According to the detection method, synchronous detection of multiple steroid hormones is realized in combination with a liquid chromatography-tandem mass spectrometry multi-reaction monitoring mode under a non-derivatization condition. According to the technical scheme, the sample pretreatment process is simplified, the detection specificity and stability are improved, and the method is suitable for multi-index quantitative detection of steroid hormones.

The invention discloses a method for evaluating the influence of human interference on reproductive health of wild animals. The method comprises the following steps: acquiring steroid hormone species and intestinal microorganism metagenome assembly genomes (MAGs) of a sentry animal excrement sample; based on the steroid hormone type, a first gene set is determined, the first gene is a metabolic enzyme gene, and metabolic enzyme participates in steroid hormone metabolism; determining a first set of coding sequences (CDSs) based on the first set of genes and the MAGs, the first CDS being from the MAGs and a coding region of the first gene comprising the first CDS; determining a first set of microorganisms (HCBs) based on the first set of CDSs, the HCBs being a set of host bacteria (HCB) of the first CDSs, the HCBs being selected from the sentry animal gut microbiota; and based on the total relative abundance of the HCBs, determining whether the human interference affects the reproductive health of the wild animals. The method is scientific and reliable, and provides a scientific decision basis for wild animal protection.

The application discloses a method for evaluating the influence of human interference on the reproductive health of wild animals. A steroid hormone species and an intestinal microorganism macro-genome assembly genome MAG of a sentinel animal fecal sample are obtained; based on the steroid hormone species, a first gene set is determined, the first gene set comprising a first gene, the first gene being a metabolic enzyme gene, the metabolic enzyme participating in steroid hormone metabolism; based on the first gene set and the MAGs, a first coding sequence CDS set is determined, the first coding sequence CDS set comprising a first CDS, the first CDS being from the MAGs, and the coding region of the first gene comprising the first CDS; based on the first coding sequence CDS set, a first microorganism set HCBs is determined, the HCBs being a collection of host bacteria HCBs of the first CDS, the HCBs being selected from the intestinal flora of the sentinel animal; based on the total relative abundance of the HCBs, it is determined whether human interference affects the reproductive health of wild animals. The method is scientific and reliable, and provides a scientific decision basis for wild animal protection.

The invention provides a mass spectrum detection system and a mass spectrum detection method for on-site rapid quantification of steroid hormones. The detection system comprises: a pretreatment module for pretreating a biological sample to obtain a first sample; the derivatization module is used for carrying out derivatization treatment on the first sample to obtain a second sample; and the capillary electrophoresis-mass spectrometry analysis module is used for sequentially carrying out electrophoretic separation and mass spectrometry detection on the second sample and carrying out qualitative and quantitative analysis on the steroid hormone in the second sample. Therefore, by adopting the detection system disclosed by the invention, the ionization efficiency and the analysis sensitivity of the steroid hormone can be improved. The capillary electrophoresis-mass spectrometry module is adopted, so that the whole analysis process is efficient and rapid, the analysis time is effectively controlled within 10 minutes, and the requirement of instant field detection can be fully met due to the high efficiency.

The invention relates to an application of an HK1 gene in mouse ovarian granular cells, in particular to an application in regulating proliferation and apoptosis of the mouse ovarian granular cells and / or steroid hormone synthesis. According to the invention, mouse ovarian granular cells are taken as a research object, and the expression of the HK1 gene is interfered to verify that the HK1 gene is taken as a key target spot for regulating and controlling the functions of the mouse ovarian granular cells so as to influence steroid hormone synthesis and cell proliferation and apoptosis processes. The invention not only provides more reliable technical support for ovarian function regulation, but also provides new thinking and technical guidance for mechanism research of reproductive diseases and preparation and development of related drugs.

The invention relates to the technical field of biomedical analysis, in particular to a clinical steroid hormone determination method which comprises the following steps: repeatedly treating human plasma by adopting a solid-phase extraction method, using octadecylsilane bonded silica gel filler, extracting to remove endogenous steroid hormone, and obtaining a human plasma blank matrix; the method comprises the following steps: respectively preparing a steroid hormone stock solution and a loratadine internal standard solution, taking the human plasma blank matrix treated in the step 1, adding the loratadine internal standard solution, treating with a 2% formic acid solution, extracting by using an HLB solid-phase extraction column, centrifugally concentrating an eluent, redissolving for LC-MS / MS analysis, carrying out sample analysis by adopting an LC-MS / MS method, and determining the content of the loratadine in the human plasma blank matrix. And detecting in an ESI positive ionization mode. Therefore, multiple steroid hormones can be detected at the same time, the coverage concentration range is wide, endogenous interference can be effectively removed, the detection accuracy is improved, the sensitivity is high, the repeatability is good, and the method is suitable for high-throughput analysis of clinical samples.

Probes that are versatile, easy to use, and provide rapid results for detecting and quantifying levels of small molecules that include steroids, hormones, antibodies, aptamers and enzymes such as various steroidal hormones like estrogen, progesterone and testosterone in samples. This is particularly useful in home and clinical settings. A probe useful in competitive assays includes a competitive ligand bound to a linker molecule bound to a detectable tag. The linker may be chemical, DNA or a combination of both.

The invention relates to the technical field of dairy product detection, in particular to a method for simultaneously detecting three steroid hormone substances in raw milk. The three steroid hormone substances are estradiol, ethinyl estradiol and estrone respectively, and the detection method comprises the following steps: 1) removing protein in raw milk to obtain a sample solution to be detected; (2) carrying out qualitative and quantitative analysis on the three steroid hormone substances in the sample solution to be detected by adopting a high performance liquid chromatography-ultraviolet detection method; wherein the qualitative analysis is based on the retention time of each substance, and the quantitative analysis is based on a standard curvilinear equation to calculate the concentration. According to the detection method, the dependence on expensive instruments and professional operators is remarkably reduced while high sensitivity and accuracy are guaranteed, the cost is low, and the method is particularly suitable for rapid screening of grassroots units and large-batch samples.

The invention relates to an application of an IDH1 gene in porcine ovarian granular cells, in particular to an application in regulating proliferation and apoptosis of the porcine ovarian granular cells and / or steroid hormone synthesis and / or reproductive performance related gene expression. According to the invention, porcine ovarian granular cells are taken as objects, and the expression of the IDH1 gene is regulated to verify that the IDH1 gene is taken as a key target spot for regulating and controlling the functions of the porcine ovarian granular cells so as to influence steroid hormone synthesis and cell proliferation and apoptosis processes. A new molecular target is provided for improving the reproductive performance of the sow, more reliable technical support is provided for regulating and controlling the ovarian function, and a new thought and technical guidance are provided for mechanism research of reproductive diseases and preparation and development of related drugs.

The application provides a kit for simultaneously detecting 90 kinds of steroid hormones and metabolites thereof in a urine sample, a detection method and a sample pretreatment method, the kit comprising 90 kinds of steroid hormones and metabolites thereof mixed standard working solution, internal standard working solution, matrix, redissolving agent and eluent, and further comprising liquid-liquid extraction agent, stabilizer, acid solution, buffer and enzymatic hydrolysis solution. 2 The application is characterized in that the R of the standard curve is greater than 0.990. The application realizes the simultaneous detection of 90 kinds of steroid hormones and metabolites thereof in a urine sample, and has the advantages of simple and rapid operation, high throughput, real-time, accurate and effective monitoring of the level of steroid hormones and metabolites thereof in human urine, and wide application prospect.

This invention discloses a novel use of posaconazole to enhance the immune response in the immunotherapy of breast cancer. Specifically, this invention provides a drug combination comprising posaconazole and a PD-L1 inhibitor. The effects of this invention are: (1) using posaconazole to prepare a drug for treating breast cancer, especially triple-negative breast cancer (TNBC), achieving "repurposing of an old drug"; (2) clarifying its core mechanism of action, clarifying that the anti-tumor effect of clinically commonly used doses of posaconazole depends on the host immune system, rather than traditional direct cytotoxicity, specifically by inhibiting CYP11A1 and other related enzymes in the tumor, thereby reducing immunosuppressive steroid hormones, providing a new drug and target for tumor immunotherapy; (3) providing a technical solution for the combined treatment of posaconazole and PD-L1 inhibitors (such as anti-PD-L1 antibodies), further enhancing anti-tumor immunity and improving the treatment effect of TNBC.

The application discloses application of follicular fluid exosome lncLIPE-AS1 in evaluation of embryo quality of patients with polycystic ovary syndrome and belongs to the field of assisted reproductive technology. The application finds that lncLIPE-AS1 is highly expressed in follicular fluid of patients with polycystic ovary syndrome, and finds that lncLIPE-AS1 is correlated with embryo quality and steroid hormone secretion of patients with polycystic ovary syndrome. Therefore, lncLIPE-AS1 can be used as a biomarker for evaluation of embryo development quality in the process of assisted reproduction of patients with polycystic ovary syndrome and prediction of success rate of assisted reproduction, and can also be used for screening of high-quality oocytes of patients with polycystic ovary syndrome and regulation of steroid hormone expression of patients with polycystic ovary syndrome.

The invention discloses a marker combination for high-salt diet related renal injury from intestinal flora and application. The marker combination is composed of dehydroepiandrosterone and Cyp1a1. The research finds that intestinal flora participates in a related marker combination (namely steroid hormone biosynthesis related metabolite dehydroepiandrosterone and Cyp1a1) of the high-salt diet related renal injury, and ROC curve analysis shows that the biomarkers have good discrimination efficiency in the high-salt diet related renal injury; the effect and application of the gene in the regulation and control of the kidney injury related to the high-salt diet by the intestinal renal axis are verified, and the gene is crucial for early diagnosis, personalized treatment and prognosis evaluation of patients with the kidney injury related to the high-salt diet.

Provided are an estimation device, an estimation method, and a program capable of estimating the steroid hormone concentration in the future from the point of time when data is measured. The estimation device estimates, by using an estimation model that receives physiological data at a certain point of time as an input and outputs an estimation value of the steroid hormone concentration after a prescribed time, a first steroid hormone concentration after a prescribed time by using first physiological data at a certain point of time. The estimation model is a model trained by using second physiological data for training at a certain point of time and a measurement value of a second steroid hormone concentration for training after a prescribed time.

The invention relates to an accelerant for up-regulating key genes of a steroid hormone synthesis pathway of scapharca broughtonii and application of the accelerant, and belongs to the technical field of aquaculture, the accelerant is prepared from the following components in parts by mass: 15-20 parts of cholesterol, 8-10 parts of linolenic acid, 10-12 parts of phosphatidylcholine, 0.5-0.8 part of zinc sulfate, 30-40 parts of L-arginine and 0.5-0.75 * 10 <-3 > part of vitamin D3. Core genes (SF-1, StAR, 17 beta-HSD14 and CYP17) of a steroid hormone synthesis pathway of scapharca broughtonii are specifically focused, synergistic up-regulation of the genes is realized through a gonad maturation accelerator, a core molecular pathway of gonad development is directly activated, and the regulation effect is accurate and stable.

The present application relates to the field of animal breeding, and particularly to a method for gender reversal of crustacean animals. The present application provides a method for gender reversal of crustacean animals, taking crustacean animals after metamorphosis, feeding with steroid hormones, and breeding. The experiment proves that sex hormones of vertebrates play a role in gender differentiation of crustacean animals, and when the concentration of MT added in feed is 2000 mg / kg, PL2 and PL25 male juvenile prawns can be induced; when the concentration of E2 added in feed is 2000 mg / kg, PL25 juvenile prawns can be induced to be female, and male juvenile prawns of PL30 can be reversed to female prawns. The experiment determines the best gender reversal period and hormone concentration in different development stages of juvenile prawns, and observes the egg-carrying "pseudo-female prawns", which lays a foundation for determining the chromosome pairing of juvenile prawns and monosex breeding.

The invention discloses a protein and a gene for degrading dexamethasone and application of the protein and the gene, and belongs to the technical field of biology. The HSD-like protein is found in a bacterial strain with a dexamethasone degradation function, the HSD-like protein is further proved to have the dexamethasone degradation function in vitro, a new theory can be provided for a steroid hormone metabolism action mechanism, an important reference is further provided for defining a metabolic pathway of steroid hormones in vivo and in vitro, and the HSD-like protein has a good application prospect. The method has important scientific research and application values. The amino acid sequence of the HSD-like protein provided by the invention is as shown in SEQ ID NO.1, and the protein can be used for degrading dexamethasone; the protein or the coding gene thereof can be used as a detection index to evaluate the sensitivity and drug resistance of patients with allergic rhinitis, allergic asthma and the like to hormone treatment, and is helpful for providing reference for clinical disease diagnosis and treatment and prognosis evaluation.

The present application relates to a preparation method of a steroid hormone blank matrix serum. The present application specifically relates to a preparation method of a steroid hormone blank matrix serum, comprising: activating HLB magnetic beads with an ammonium acetate methanol solution; extracting steroid hormones existing in serum by mixing the serum with the activated HLB magnetic beads; and collecting a first supernatant by applying a magnetic field to adsorb the HLB magnetic beads. The present application also relates to a method for extracting steroid hormones in serum and a method for regenerating HLB magnetic beads for extracting steroid hormones. The method of the present application is simple, fast and safe to operate; can meet the needs of automatic equipment, and is especially suitable for automatic mass production; and the prepared blank matrix serum is suitable for use as a blank control or a blank diluent in detection.

The invention relates to a compound shown in a formula (I). The compound is capable of inhibiting steroid hormone dependent diseases and diseases of CYP11A1. Also included are pharmaceutical compositions comprising the compounds of Formula (I) and methods for treating conditions associated with CYP11A1 activity.

The application discloses a 21-hydroxylase deficiency screening kit and application thereof, relates to the blood analysis technical field, and a steroid hormone marker composition of 21-hydroxylase deficiency, wherein the nine markers comprise 17alpha-hydroxyprogesterone, androstenedione, 11-deoxycortisol, 21-deoxycortisol, cortisol, corticosterone, progesterone, dihydrotestosterone and 11-deoxycorticosterone; the kit can be used for screening 21-hydroxylase deficiency, 11beta-hydroxylase deficiency, 17alpha-hydroxylase deficiency and other types of diseases; the kit comprises detection components for detecting the hormones; compared with a traditional time-resolved fluorescence immunoassay method, the LC-MS / MS multi-index combined detection scheme adopted in the application can significantly reduce false positive results; and invalid recall, unnecessary family anxiety and medical burden caused by false positive results are greatly reduced.

This invention discloses a method for detecting steroid hormones using furoyl chloride derivatization-liquid chromatography-tandem mass spectrometry. This invention utilizes furoyl chloride to derivatize steroid hormones, and the combination with liquid chromatography-tandem mass spectrometry enables highly sensitive and accurate quantification of trace amounts of steroid hormones such as estrone and estradiol in biological matrices. Compared with existing technologies, the method for detecting steroid hormones using furoyl chloride derivatization-liquid chromatography-tandem mass spectrometry provided by this invention exhibits high recovery, a wide linear range, good linearity, low detection limit, and mild reaction conditions with a simplified reaction procedure.

The invention discloses a gene for regulating chicken follicular cell proliferation and steroid hormone synthesis and application thereof, and belongs to the technical field of biotechnology and genetic breeding. Cell tests prove that the recombinant protein can improve the proliferation activity of chicken primary follicular membrane cells by 20-30%, increase the S-phase number by 14%, increase the proliferation number by 1.6 times, increase the estrogen secretion amount by 11% and increase the androgen secretion amount by 14% compared with a control group, and the recombinant protein can regulate and control the key links of follicular development. A new thought of cutting into a protein function is provided for poultry breeding; by detecting and selecting the IGFBP1 gene, the breeding efficiency of high-quality laying hens can be improved. Along with increase of breeding requirements of high-quality poultry varieties, the technology can shorten the breeding period of the new high-quality laying hen variety (commercial line), has wide application prospects, can assist in coping with related breeding challenges, improves breeding economic benefits, and provides support for innovation of a poultry genetic breeding technology.

Described herein are methods of promoting remyelination in a subject having a demyelinating disease by administering to the subject a combination of a steroid hormone and a Hedgehog signaling pathway modulator. Also described are methods of administering a combination of pharmaceutical products, wherein the combination of pharmaceutical products is in a composition suitable for nasal administration.