47 results about "Steroidal hormones" patented technology

The invention discloses a steroid hormone degrading bacterium as well as a screening method and application thereof, and relates to the field of biological medicines, the steroid hormone degrading bacterium comprises klebsiella pneumoniae (KP2022 Klebsiella pneumoniae) with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M2025478. The steroid hormone degrading bacterium provided by the invention has the capability of efficiently degrading dexamethasone, is beneficial to degrading dexamethasone in the environment, is conveniently obtained by adopting a screening method, and provides a sample for research on degradation of steroid hormones.

The present invention relates to a method for detecting steroid hormones in serum, comprising the following steps: (1) adding 0.1 to 20 mg of a magnetic solid phase extractant to each milliliter of a sample to be tested, mixing, magnetically separating, and removing liquid; adding a washing solution, mixing, magnetically separating, and removing liquid; adding an eluent for elution, mixing, magnetically separating, and collecting the eluent to obtain a test solution; (2) detecting the hormone content in the test solution by liquid chromatography and tandem mass spectrometry; wherein the hormone is selected from any one of testosterone, androstenedione, dihydrotestosterone, dehydroepiandrosterone sulfate, and 17α-hydroxyprogesterone or a combination thereof; the magnetic solid phase extractant comprises a magnetic core ferrosoferric oxide, a middle layer structure silica wrapping the magnetic core, and an outer layer mesoporous structure, wherein the outer layer mesoporous structure is selected from any one of polystyrene and polystyrene divinylbenzene or a combination thereof. The present invention has the characteristics of good stability, high accuracy and sensitivity, strong specificity, high recovery rate, simple and controllable operation, fast analysis speed, etc., and can be used for simultaneous quantitative detection of multiple hormones in clinical practice.

Among hospital–acquired infections, Pseudomonas aeruginosa-associated urinary tract infections (UTIs) are mainly caused by indwelling urethral catheters (catheter-associated UTIs or CAUTIs) and are difficult to treat, resulting in high rates of morbidity among hospitalized patients. While antibiotics can successfully treat bacteria in the bladder lumen, they are inefficient at crossing stratified urothelium plasma membranes to kill persistent intracellular bacterial communities (IBCs). Herein, UTI IBCs are targeted by locally delivering the antibiotic gentamicin and / or the steroid hormone estradiol via polymeric nanogels conjugated with a cell-penetrating peptide Cys-Gly-Lys-Arg-Lys (CGKRK - SEQ ID NO:1). This approach delivered more intracellular gentamicin compared to drug delivered in solution in vitro and more estradiol compared to conventional methods. In an acute UTI murine model, the nanogel cell-penetrating peptide drug delivery system facilitated the transport of gentamicin into the urothelium and resulted in > 90% clearance of a uropathogenic P. aeruginosa clinical strain in vivo.

The invention relates to a method for simultaneously detecting various steroid hormones in serum, which comprises the following steps: extracting hormones in serum by a liquid-liquid extraction method; a derivatization reagent is added into the extracted hormone, an oximation reaction is carried out, and the derivatization reagent comprises quaternary ammonium oxygen amine; the hormone after oximation reaction is injected into a two-dimensional liquid chromatography-tandem mass spectrometry system for detection, the hormone is enriched on a one-dimensional chromatographic column, and then the enriched hormone is subjected to back flushing and transferred to a two-dimensional chromatographic column for analysis. According to the method, the backwashing transfer two-dimensional liquid chromatography-tandem mass spectrometry and the derivatization reaction are combined, various steroid hormones including progestational hormone and androgen can be detected at the same time, and the method has the advantage of being high in detection sensitivity.

The present application relates to a compound represented by formula (I), and the compound can inhibit steroid hormone-dependent disorders and diseases of CYP11A1. The present application further comprises a pharmaceutical composition comprising the compound represented by formula (I) and a method for treating a condition related to CYP11A1 activity.

The present disclosure relates to a method for detecting vitamin D or a steroid hormone and a blood sample pretreatment kit. The method for detecting vitamin D in blood comprises pretreating a blood sample to obtain a test sample, and detecting the test sample by liquid chromatography tandem mass spectrometry, wherein the pretreatment comprises: adding an internal standard solution and a magnetic bead reagent to the blood sample to obtain a mixed solution and incubating to allow the target analyte to bind to the surface of the magnetic beads; separating the magnetic beads combined with the target analyte; and eluting the target analyte from the surface of the magnetic beads to obtain the test sample, wherein the mixed solution comprises methanol in an amount of 45vol% to 60vol%, or ethanol in an amount of 30vol% to 50vol%. The method can significantly reduce non-specific adsorption of magnetic beads, and improve the accuracy and sensitivity of detection.

The invention relates to the technical field of in-vitro diagnostic reagents, in particular to a steroid hormone-containing immunosuppressive agent precise monitoring treatment kit, and discloses a joint detection and correction method for precise monitoring of a steroid hormone-containing immunosuppressive agent. Comprising the following steps: step 1, collecting clinical samples to be detected, and subpackaging each collected sample into two equal parts; 2, carrying out immunological quantitative determination on the first equal part of sample by adopting an immunological kit to obtain an immunological determination value; 3, the second equal sample corresponding to the step 2 is subjected to reference quantitative determination through liquid chromatography-tandem mass spectrometry, a mass spectrometry determination value is obtained, and mass spectrometry quantification uses an isotope labeling internal standard corresponding to each target analyte and is carried out according to a standardized sample pretreatment process. According to the method, high throughput and operation convenience of the immunoassay are guaranteed, meanwhile, the accuracy equivalent to that of mass spectrometry is provided, and a reliable basis is provided for clinical individualized medication.

The present disclosure provides novel compositions for biologically active compounds that are administered through injection. The active compounds include, but are not limited to steroid hormones, diarylheptanoids, and flavonoids. These compositions are distinguished from the prior art through the addition of an oil to the overall composition, which results in a biphasic formulation. The oil serves to enhance the efficiency of delivery of the formulation to a human or animal subject, and reduces discomfort associated with the injection process. Optionally, one or more additional substances, each of which is soluble in oil, can be added to the oil to enable the deliverable of multiple active compounds in a single injection.

The present invention addresses the problem of providing: a steroid hormone detection method and steroid hormone detection kit that are capable of avoiding the influence of steroid hormone-binding proteins during the measurement of steroid hormones in a biological sample; a compound and inclusion complex that can be used in the abovementioned detection method and detection kit; and a composition containing the abovementioned compound or inclusion complex. The present invention provides a steroid hormone detection method involving: bringing a hydrogen halide salt or cyclodextrin inclusion complex of a compound represented by formula [1] into contact with a complex of a steroid hormone-binding protein and steroid hormones in a biological sample; and detecting steroid hormones. In the formula, R1, R2, and R3 are each independently a hydrogen atom or 1-3C alkyl group. R4 represents a halogen atom, a 1-3C alkyl group, a phenyl group, a methoxy group, or an ethoxy group.

The invention relates to the technical field of livestock breeding biology, and discloses a method for promoting porcine ovary granular cell proliferation, which comprises the following steps: adding a porcine follicular fluid exosome into a porcine ovary granular cell culture system, and acting for a certain time at an effective concentration, so as to improve the proliferation activity of granular cells. After the exosome treatment, the apoptosis rate of granular cells can be reduced, the cell survival rate can be improved, and the granular cells can be promoted to secrete steroid hormones, including the estradiol level and the expression of progesterone synthetase. Cell experiments prove that the follicular fluid exosome can effectively improve the activity of granular cells, promote the process of a cell cycle, inhibit cell apoptosis and remarkably enhance the synthesis capacity of steroid hormones such as estradiol and progesterone, and a new intervention strategy is provided for improving follicular development and reproductive performance of sows.

The invention particularly relates to a steroid hormone magnetic particle mass spectrometry detection kit and a detection method thereof. The kit comprises a reagent 0, a reagent 1, a reagent 2, a reagent 3, a reagent 4, a calibration product and a quality control product, the reagent 0 is a magnetically attractable steroid hormone reagent 0 working solution, the reagent 1 is a steroid hormone isotope internal standard reagent, the reagent 2 and the reagent 3 are elution reagents, and the reagent 4 is an elution reagent. By sequentially carrying out adsorption, leaching and elution treatment, one-time magnetic solid-phase extraction of steroid hormones in a biological sample is realized, and a supernatant for liquid chromatography-tandem mass spectrometry detection is obtained. According to the detection method, synchronous detection of multiple steroid hormones is realized in combination with a liquid chromatography-tandem mass spectrometry multi-reaction monitoring mode under a non-derivatization condition. According to the technical scheme, the sample pretreatment process is simplified, the detection specificity and stability are improved, and the method is suitable for multi-index quantitative detection of steroid hormones.

The invention relates to a preparation method of steroid hormone blank matrix serum. The invention particularly relates to a preparation method of steroid hormone blank matrix serum. The preparation method comprises the following steps: activating HLB (Hydrophile Lipophile Balance) magnetic beads by using an ammonium acetate methanol solution; the method comprises the following steps: mixing serum with activated HLB magnetic beads, and extracting steroid hormones in the serum; and applying a magnetic field to adsorb the HLB magnetic beads, and collecting a first supernatant. The invention also relates to a method for extracting the steroid hormone in the serum and a method for regenerating the HLB magnetic beads for extracting the steroid hormone. The method is simple, rapid and safe to operate; the requirements of automatic equipment can be met, and the method is particularly suitable for automatic large-scale preparation; meanwhile, the prepared blank matrix serum is suitable for being used as a blank control or a blank diluent in detection.

The invention discloses a method for evaluating the influence of human interference on reproductive health of wild animals. The method comprises the following steps: acquiring steroid hormone species and intestinal microorganism metagenome assembly genomes (MAGs) of a sentry animal excrement sample; based on the steroid hormone type, a first gene set is determined, the first gene is a metabolic enzyme gene, and metabolic enzyme participates in steroid hormone metabolism; determining a first set of coding sequences (CDSs) based on the first set of genes and the MAGs, the first CDS being from the MAGs and a coding region of the first gene comprising the first CDS; determining a first set of microorganisms (HCBs) based on the first set of CDSs, the HCBs being a set of host bacteria (HCB) of the first CDSs, the HCBs being selected from the sentry animal gut microbiota; and based on the total relative abundance of the HCBs, determining whether the human interference affects the reproductive health of the wild animals. The method is scientific and reliable, and provides a scientific decision basis for wild animal protection.

The invention discloses application of cinnamyl aldehyde, and cinnamyl aldehyde can regulate synthesis and secretion of androgen testosterone and can be applied to related products for regulating synthesis and secretion of androgen testosterone. In addition, tests prove that cinnamyl aldehyde increases synthesis and secretion of testicular interstitial cell androgen by improving activity of a steroid hormone synthesis nuclear receptor promoter and up-regulating expression of steroid hormone synthetase.

The application discloses a method for evaluating the influence of human interference on the reproductive health of wild animals. A steroid hormone species and an intestinal microorganism macro-genome assembly genome MAG of a sentinel animal fecal sample are obtained; based on the steroid hormone species, a first gene set is determined, the first gene set comprising a first gene, the first gene being a metabolic enzyme gene, the metabolic enzyme participating in steroid hormone metabolism; based on the first gene set and the MAGs, a first coding sequence CDS set is determined, the first coding sequence CDS set comprising a first CDS, the first CDS being from the MAGs, and the coding region of the first gene comprising the first CDS; based on the first coding sequence CDS set, a first microorganism set HCBs is determined, the HCBs being a collection of host bacteria HCBs of the first CDS, the HCBs being selected from the intestinal flora of the sentinel animal; based on the total relative abundance of the HCBs, it is determined whether human interference affects the reproductive health of wild animals. The method is scientific and reliable, and provides a scientific decision basis for wild animal protection.

The invention relates to the technical field of analysis and detection, in particular to a detection method for quantitative urine steroid hormone based on efficient enzymolysis. The invention establishes a detection method for accurately quantifying 35 steroid hormones in urine by combining a high performance beta glucuronide enzymatic dissociation method with high performance liquid chromatography-tandem mass spectrometry. According to the method, the pretreatment time required by clinical determination of urine steroid hormones can be remarkably shortened, single-needle quantitative detection of 35 steroid hormones is realized, and the method has a wide application prospect.

The invention provides a mass spectrum detection system and a mass spectrum detection method for on-site rapid quantification of steroid hormones. The detection system comprises: a pretreatment module for pretreating a biological sample to obtain a first sample; the derivatization module is used for carrying out derivatization treatment on the first sample to obtain a second sample; and the capillary electrophoresis-mass spectrometry analysis module is used for sequentially carrying out electrophoretic separation and mass spectrometry detection on the second sample and carrying out qualitative and quantitative analysis on the steroid hormone in the second sample. Therefore, by adopting the detection system disclosed by the invention, the ionization efficiency and the analysis sensitivity of the steroid hormone can be improved. The capillary electrophoresis-mass spectrometry module is adopted, so that the whole analysis process is efficient and rapid, the analysis time is effectively controlled within 10 minutes, and the requirement of instant field detection can be fully met due to the high efficiency.

The invention relates to an application of an HK1 gene in mouse ovarian granular cells, in particular to an application in regulating proliferation and apoptosis of the mouse ovarian granular cells and / or steroid hormone synthesis. According to the invention, mouse ovarian granular cells are taken as a research object, and the expression of the HK1 gene is interfered to verify that the HK1 gene is taken as a key target spot for regulating and controlling the functions of the mouse ovarian granular cells so as to influence steroid hormone synthesis and cell proliferation and apoptosis processes. The invention not only provides more reliable technical support for ovarian function regulation, but also provides new thinking and technical guidance for mechanism research of reproductive diseases and preparation and development of related drugs.

The invention relates to the technical field of biomedical analysis, in particular to a clinical steroid hormone determination method which comprises the following steps: repeatedly treating human plasma by adopting a solid-phase extraction method, using octadecylsilane bonded silica gel filler, extracting to remove endogenous steroid hormone, and obtaining a human plasma blank matrix; the method comprises the following steps: respectively preparing a steroid hormone stock solution and a loratadine internal standard solution, taking the human plasma blank matrix treated in the step 1, adding the loratadine internal standard solution, treating with a 2% formic acid solution, extracting by using an HLB solid-phase extraction column, centrifugally concentrating an eluent, redissolving for LC-MS / MS analysis, carrying out sample analysis by adopting an LC-MS / MS method, and determining the content of the loratadine in the human plasma blank matrix. And detecting in an ESI positive ionization mode. Therefore, multiple steroid hormones can be detected at the same time, the coverage concentration range is wide, endogenous interference can be effectively removed, the detection accuracy is improved, the sensitivity is high, the repeatability is good, and the method is suitable for high-throughput analysis of clinical samples.

The invention provides a kit for simultaneously detecting six steroid hormones and application of the kit. The steroid hormones comprise testosterone, androstenedione, dehydroepiandrosterone, dehydroepiandrosterone sulfate, dihydrotestosterone and 17alpha-hydroxyprogesterone. The kit comprises extract liquor, a mobile phase additive and a standard quality control product. The kit provided by the invention can be used for simultaneously detecting testosterone, androstenedione, dehydroepiandrosterone, dehydroepiandrosterone sulfate, dihydrotestosterone and 17alpha-hydroxyprogesterone, and has the advantages of high accuracy, low quantitation limit and wide detection range.

The invention discloses application of silibinin in preparation of a medicine for treating autoimmune hepatitis, and belongs to the field of biological medicine application. The silybin has a good liver protection effect, and a pathological experiment combined with a related mouse disease model in development of autoimmune liver injury pathogenesis research finds that the silybin can well relieve inflammation caused by the autoimmune liver injury, so that the silybin can replace or assist a traditional steroid hormone therapy, and the silybin has a good application prospect. And side effects caused by traditional therapy are reduced.

Probes that are versatile, easy to use, and provide rapid results for detecting and quantifying levels of small molecules that include steroids, hormones, antibodies, aptamers and enzymes such as various steroidal hormones like estrogen, progesterone and testosterone in samples. This is particularly useful in home and clinical settings. A probe useful in competitive assays includes a competitive ligand bound to a linker molecule bound to a detectable tag. The linker may be chemical, DNA or a combination of both.

The invention relates to the technical field of dairy product detection, in particular to a method for simultaneously detecting three steroid hormone substances in raw milk. The three steroid hormone substances are estradiol, ethinyl estradiol and estrone respectively, and the detection method comprises the following steps: 1) removing protein in raw milk to obtain a sample solution to be detected; (2) carrying out qualitative and quantitative analysis on the three steroid hormone substances in the sample solution to be detected by adopting a high performance liquid chromatography-ultraviolet detection method; wherein the qualitative analysis is based on the retention time of each substance, and the quantitative analysis is based on a standard curvilinear equation to calculate the concentration. According to the detection method, the dependence on expensive instruments and professional operators is remarkably reduced while high sensitivity and accuracy are guaranteed, the cost is low, and the method is particularly suitable for rapid screening of grassroots units and large-batch samples.

The invention discloses application of glycine hyodeoxycholic acid in regulation and control of follicular granule cell viability and steroid hormone secretion. According to the application disclosed by the invention, a pig follicular granule cell in-vitro culture technology is utilized, the cell viability and steroid secretion condition of the follicular granule cells after exogenous addition of the GHDCA are detected, and a result shows that the GHDCA can enhance the cell viability of the pig follicular granule cells in a dose-dependent manner, and meanwhile, the GHDCA can promote the secretion of estradiol and inhibit the secretion of progesterone in a dose-dependent manner. The result shows that the GHDCA can regulate and control the activity of follicular granule cells and the secretion of steroid hormones. According to the invention, a foundation is laid for the research of the GHDCA on the molecular mechanism of promoting the secretion of follicular granular cell hormone, and a possible way is also provided for the development and estrus regulation of pig follicles.

The present invention belongs to the field of clinical testing, and specifically relates to a kit and method for simultaneously detecting 18 types of steroid hormones based on a double derivatization technology. An internal standard solution is added to the series of calibration solution, the series of quality control solution and the serum sample respectively; a protein precipitant and a sample extract are then added to each sample, and sample enhancement solution 1 is added to the extract to perform an oximation reaction; sample enhancement solution 2 and a sample conditioning solution are further added to perform an esterification reaction; after the reaction is completed, the reaction solution is centrifuged, and the supernatant is taken, and the supernatant is subjected to LC-MS / MS detection. The present invention adopts a double derivatization method to simultaneously detect multiple steroid hormones. There is no need for positive and negative switching during mass spectrometry detection, and no high-value consumables such as magnetic beads or SPE are required. The simultaneous detection of estrogen and other steroid hormones can be achieved on a conventional liquid chromatography-tandem mass spectrometry platform, which expands the clinical applicability of the steroid hormone detection method.

A self-sealing trabeculectomy, which solves the problems in existing scleral flap suturing. The self-sealing trabeculectomy includes the following steps: S1: before a surgery, conducting disinfection, draping and eye speculum placement, and conducting local infiltration anesthesia under a surgical eye conjunctiva; S2: making a conjunctival flap; S3: making a 4 mm×3 mm or 4 mm×4 mm scleral flap; S4: resecting a root of an iris and trabecular tissues, smoothing the scleral flap with a restoration instrument, and suturing the bulbar conjunctiva with a nylon thread to reach a watertight state; S5: removing a suspending suture for the rectus or a cornea, reducing the eye speculum to weaken a tension of the eye speculum, and forming an anterior chamber; S6: taking out the eye speculum; and S7: after the surgery, conventionally locally applying a corticosteroid hormone and a non-steroid anti-inflammatory drug, keeping a filtration channel unobstructed.

The present disclosure provides formulations for biologically active molecules including steroid hormones, diarylheptanoids, vitamins and flavonoids. These formulations are distinguished from the prior art through the inclusion of magnesium stearate or a salt with comparable properties along with a phospholipid carrier complex. These formulations provide for more convenient modes of delivery, such as via orally consumed capsules containing a solid powder. The present disclosure also provides for the use of combinations of biologically active molecules flavonoids and / or steroid hormone, including the combined delivery of the hormone testosterone and curcuminoid compounds to human and / or animal subjects.

The invention relates to compounds of formula (I) and pharmaceutically acceptable salts thereofwherein R1 to R4 are as defined in the claims. The invention further relates to their use as inhibitors of 17β-HSD1 and in treatment or prevention of steroid hormone dependent diseases or disorders, such as steroid hormone dependent diseases or disorders requiring the inhibition of the 17β-HSD1 enzyme and / or requiring the lowering of the endogenous estradiol concentration. The present invention also relates to the preparation of the aforementioned compounds and to pharmaceutical compositions comprising as an active ingredient(s) one or more of the aforementioned compounds or pharmaceutically acceptable salts thereof.

The invention relates to an application of an IDH1 gene in porcine ovarian granular cells, in particular to an application in regulating proliferation and apoptosis of the porcine ovarian granular cells and / or steroid hormone synthesis and / or reproductive performance related gene expression. According to the invention, porcine ovarian granular cells are taken as objects, and the expression of the IDH1 gene is regulated to verify that the IDH1 gene is taken as a key target spot for regulating and controlling the functions of the porcine ovarian granular cells so as to influence steroid hormone synthesis and cell proliferation and apoptosis processes. A new molecular target is provided for improving the reproductive performance of the sow, more reliable technical support is provided for regulating and controlling the ovarian function, and a new thought and technical guidance are provided for mechanism research of reproductive diseases and preparation and development of related drugs.

The application provides a kit for simultaneously detecting 90 kinds of steroid hormones and metabolites thereof in a urine sample, a detection method and a sample pretreatment method, the kit comprising 90 kinds of steroid hormones and metabolites thereof mixed standard working solution, internal standard working solution, matrix, redissolving agent and eluent, and further comprising liquid-liquid extraction agent, stabilizer, acid solution, buffer and enzymatic hydrolysis solution. 2 The application is characterized in that the R of the standard curve is greater than 0.990. The application realizes the simultaneous detection of 90 kinds of steroid hormones and metabolites thereof in a urine sample, and has the advantages of simple and rapid operation, high throughput, real-time, accurate and effective monitoring of the level of steroid hormones and metabolites thereof in human urine, and wide application prospect.