58 results about "Methyltestosterone" patented technology

The invention discloses a color and luster improving feed for rhodeus sinensis and a preparation method thereof, and relates to the technical field of processing of feed for aquarium fish. The feed is prepared from the following raw materials in parts by weight: 10 to 30 parts of fish meal, 10 to 30 parts of dried flour weevil, 10 to 20 parts of flour, 1 to 10 parts of pumpkin powder, 10 to 30 parts of corn flour, 1 to 10 parts of wheat bran, 1 to 10 parts of kelp powder, 0.1 to 10 parts of calendula officinalis, 0.1 to 10 parts of beer yeast, 0.1 to 5 parts of monocalcium phosphate, 0.1 to 2 parts of glycine betaine, 0.1 to 5 parts of spirulina powder, 0.1 to 5 parts of paprika, 0.1 to 5 parts of garlic powder, 1 to 8 parts of blood worm powder, 1 to 5 parts of fish oil, 1 to 5 parts of soybean oil, 0.01 to 0.05 part of mildew preventive, and 0 to 0.01 part of methyltestosterone. The color and luster improving feed for rhodeus sinensis can promote the growth and development of fish, and improve the immunity of the fish, and shows an effect of improving and remaining color for a long term; the feed subjected to drying and packing is convenient to use, and can be stored for a long time and transported in a long distance; the color and luster improving feed for rhodeus sinensis is a safe and reliable green and environment-friendly feed without any residue and pollution.

The invention relates to a male trait induction method for pelodiscus sinensis in embryonic periods. The male trait inducer is characterized in that through dissolving an aromatase inhibitor and an adjuvant into alcohol with the purity of 92-98%, the inducer with an aromatase inhibitor concentration of 50-150 mu g/mu l and an adjuvant concentration of 50-150 mu g/mu l is obtained; and the adjuvant is selected from testosterone, methyltestosterone or testosterone propionate. According to the induction method provided by the invention, the male induction rate can be more than 95% just through spraying the inducer two times at the sex determination key time point in embryonic development, and meanwhile, the normal development and low drug residues of pelodiscus sinensis gonads are ensured; and the induction method provided by the invention is easy to operate, low in cost, and remarkable in economic benefit, thereby being a sex control technology which is worthy of popularization in the existing pelodiscus sinensis breeding.

Compositions are disclosed. One embodiment of a composition comprises a first antibody having an affinity for an antigen and a second antibody having an affinity for the first antibody, wherein at least one antibody is conjugated to a marker, and wherein the antigen is not present in the composition. Further disclosed are methods of using compositions according to the invention for analyzing a biological sample by antibody profiling for identifying forensic samples or detecting the presence of an analyte. In embodiments of the invention, the analyte is a drug, such as marijuana, cocaine, methamphetamine, methyltestosterone, or mesterolone. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, may be coupled to a test for identity such that the results of the assay may be positively correlated to the subject's identity.

The invention discloses a sex control method for Chinese soft-shelled turtles, which includes: firstly, controlling the incubation temperature to be from 23 DEG C to 35 DEG C; and secondly, dropwise adding methyltestosterone to an animal pole of each fertilized egg. By means of temperature control and application of the methyltestosterone, the sex control method evidently increases a male proportion of Chinese soft-shelled turtles and meets market requirements.

The invention provides a preparation method of methyltestosterone. 4AD short for 4-androstenedione is taken as a raw material, and etherate is synthesized firstly as follows: 4AD and triethyl orthoformate are subjected to an acid catalyzed reaction in a low-carbon alcohol organic solvent, and 3-ethoxy-androst-3,5-diene-17-one as the etherate is obtained; then a Grignard product is synthesized as follows: a Grignard reagent methyl magnesium halide and the etherate are placed in an organic solvent, the 17-position ketone group of the etherate and the Grignard reagent are subjected to addition, and the Grignard product 3-ethoxy-17a-methyl-androst-3,5-diene-17-ol is obtained through hydrolysis; then the Grignard product is subjected to an acid catalyzed hydrolysis in an organic solvent, and crude methyltestosterone is obtained; the crude methyltestosterone is decolorized by activated carbon in C4-below low-carbon alcohol and recrystallized, the methyltestosterone is obtained, HPLC content is 99.0%-99.5%, and the total yield of synthesis weight is 75%-78%. According to the method, the raw materials are widely sourced, the process is simple and convenient to operate, the product yield is high, the purity is good, the solvent recovery rate is high in reaction and technological processing, and the method is economical and environment-friendly.

A method is disclosed for analyzing a biological sample by antibody profiling for identifying forensic samples or for detecting the presence of an analyte. In an illustrative embodiment of the invention, the analyte is a drug, such as marijuana, cocaine, methamphetamine, methyltestosterone, or mesterolone. The method involves attaching antigens to the surface of a solid support in a preselected pattern to form an array wherein the locations of the antigens are known; contacting the array with the biological sample such that a portion of antibodies in the sample reacts with and binds to antigens in the array, thereby forming immune complexes; washing away antibodies that do form immune complexes; and detecting the immune complexes, thereby forming an antibody profile. Forensic samples are identified by comparing a sample from an unknown source with a sample from a known source. Further, an assay, such as a test for illegal drug use, can be coupled to a test for identity such that the results of the assay can be positively correlated to the subject's identity.

The invention provides a colloidal gold test strip for detecting methyltestosterone residue. The colloidal gold test strip includes a sample absorption pad, a conjugate release pad, a reaction film, a water absorption pad and a bottom plate. The reaction film has a detection line coated with a methyltestosterone semiantigen-carrier protein conjugate and a quality control line coated with a goat-anti-mouse anti-antibody, and the conjugate release pad is sprayed with a methyltestosterone monoclonal antibody-colloidal gold marker. The invention also provides a method for detecting methyltestosterone by using the colloidal gold test strip, which comprises the following steps: pre-treating a sample, then detecting the sample by the colloidal gold test strip, and finally analyzing the detection result. The colloidal gold test strip can be used for detecting the methyltestosterone residue in the animal tissue samples such as pork, chicken, fish and shrimp, has the characteristics of simple operation, high sensitivity, fast detection speed and low cost, etc., and is suitable for screening of a large number of samples and field monitoring.

The invention provides a method for synthesizing 17alpha-hydroxyprogesterone.17beta-cyano-5-androstene-17-alcohol-3,3-ethylene dihydroxy ketal (an intermediate II for short) is adopted as a raw material, and cheap cyclohexanone cyanohydrin is adopted as an inhibitor of a main impurity methyltestosterone. Due to the fact that a cyclohexanone cyanohydrin structure is similar to a D-loop structure in the molecular structural formula of the intermediate II and can replace a D loop to react with OH<1> in a Grignard reaction system, generated methyltestosterone is greatly reduced. Under the catalytic action of anhydrous lithium chloride or CuCl, the reaction temperature is reduced to -15 DEG C to -10 DEG C, and other generated impurities can be reduced at the low temperature. Therefore, the product quality is improved by controlling Grignard reaction conditions, the content of crude HPLC can reach 98% or above, and the total weight yield ranges from 84% to 88% and is improved by 10% to 14% compared with a traditional process.

The invention discloses a specific monoclonal antibody capable of distinguishing nandrolone, methyltestosterone, testosterone and trenbolone at the same time. The monoclonal antibody is secreted by a hybridoma cell strain NT4D12 of which the preservation number is CCTCC No.C201494. The invention further discloses an enzyme-linked immunosorbent assay method and kit for detecting the androgens. Compared with the prior art, the monoclonal antibody prepared by the invention can be used for distinguishing four androgens namely the nandrolone, the methyltestosterone, the testosterone and the trenbolone, is high in detection sensitivity and good in specificity. The ELISA method and the kit, disclosed by the invention, have the advantages of high detection sensitivity, high accuracy and high precision.

The invention discloses a pseudo-male and full-female nile tilapia fingerling production method. The method includes the following steps that the step 1, fodder including methyltestosterone is fed continuously for 15 days after nile tilapia opens mouth, and phenotype full-male tilapia is obtained and bred to be sexually mature; the step 2, the phenotype full-male tilapia is used as male parents, common female nile tilapia is used as female parents, and compatriot families are established in the ratio of 1:1; the step 3, 100 fries are selected randomly from each family for methyltestosterone treatment, in addition, 100 fries commonly bred are selected randomly as a comparison set, the ratio of female tilapia and male tilapia in the comparison set is counted, medication treatment set male tilapia corresponding to the full-female comparison set is the pseudo-male tilapia with sex chromosome XX, and the pseudo-male tilapia is selected and remains and is bred to be sexually mature; the step 4, the sex chromosome of offspring bred through the pseudo-male tilapia and the common tilapia is XX, large-scale fingerling production of the full-female tilapia can be achieved through common breeding, and mass production of the pseudo-male tilapia can be achieved through methyltestosterone treatment. According to the method, the quality of fingerling of the tilapia can be improved, the fingerling production efficiency can be improved, and fingerling production cost can be reduced.

The invention discloses a method for inducing pseudo milters of gynogenesis spotted maigre and belongs to the technical field of aquatic biology. The pseudo milters of the spotted maigre can be obtained by using male hormone for induction during the breeding process of the gynogenesis spotted maigre, and the male hormone is methyltestosterone. The method is reasonable in design, the methyltestosterone is utilized for induction, induction steps are scientifically and reasonably limited, the finally obtained male rate of the gynogenesis spotted maigre can reach more than 86%, and theoretical and technical guidance is provided for qualitative breeding of the gynogenesis spotted maigre.

The invention discloses a method for preparing a methyltestosterone molecularly-imprinted polymer, in particular a precipitation polymerization preparation method for the methyltestosterone molecularly-imprinted polymer. The preparation method comprises the following steps of: adding methyltestosterone and polymerization monomer in a ratio of 1:3-1:8 into a pore-forming agent, and stirring the mixture for 0.5 to 3 hours at a low temperature; then adding a cross linker and an initiator into the mixture, performing thermal initiated polymerization, centrifuging the solution at a high speed, and then taking sediment; and extracting the sediment by an acid organic solvent to remove template molecules, then extracting the sediment by using an organic solvent to remove the residual acid substances, and drying the sediment in vacuum to obtain a product. The method has the advantages of simple operation, good repeatability, easy control of processes and the like; the obtained molecularly-imprinted polymer has controllable particle diameter and uniform size; and the polymer has good identification capability and selection property on the methyltestosterone, and has good bonding capability and separating capability.

The invention relates to a preparation method of a molecularly imprinted polymer of methyltestosterone, and the preparation method comprises the following steps: dissolving the methyltestosterone into a pore-forming agent with the proportion of 6-60ml/mmol, then mixing with a polymerized monomer according to the proportion of 1:3-1:8, and oscillating for 0.5-4h; adding a cross-linking agent and an initiator, uniformly mixing, adding water solution of dispersant into the mixed solution dropwise, introducing inert gas for 10-20min, sealing and placing in a water bath at 55-65 DEG C, simultaneously stirring for 18-30h, carrying out solid-liquid separation and taking the solid phase part; and extracting with an acid organic solvent, further extracting with a pure organic solvent and obtaining a product. The method has the advantages of simple operation, easy control of the process, low cost and the like, and can overcome the shortcomings of non-uniform particle size of the obtained polymer, great loss and the like; and the obtained molecularly imprinted polymer has large specific surface area and can be directly used without grinding and screening.

Feed for aquarium fish is prepared from, by mass, 20%-30% of wheat, 10%-20% of fish meal, 10%-20% of chicken liver meal, 10%-20% of silkworm chrysalis meal, 10%-20% of whole egg powder, 5%-10% of dried shrimp meal, 0.01%-1% of beta-carotene, 2%-3% of dehydrated spinach, 2%-3% of red yeast rice powder, 1%-2% of soybean petide and 0.5%-1% of 17 alpha-methyltestosterone which is purchased from Shanghai Yubo Bio-Technology Limited and is more than or equal to 98% in purity. The feed provides comprehensive and balanced nutrients for growth of the aquarium fish, and the 17 alpha-methyltestosterone activates LDL receptors on the surfaces of chromatophores at the same time, so that the lipoprotein uptake of the chromatophores is increased, the amount of carotenoid carried by lipoprotein and deposited in the chromatophores is increased, and the physical fitness and the body color of the aquarium fish are enhanced.

The invention relates to the field of genetic engineering, in particular to a monopterus albus AKR (aldo-keto reductase) gene with nucleotide and amino acid sequences shown in SEQ ID NO:1 and SEQ ID NO:2. A recombinant expression vector of a monopterus albus Eakr gene is constructed with methods in molecular biology and genetic engineering, induction expression and affinity chromatography are performed, and purified recombinant expression protein is obtained. Monopterus albus Eakr recombinant protein can catalyze different substrates such as methylglyoxal, acetone, progesterone, methyltestosterone and the like and has theoretical guidance significance and application reference value for industrial application of AKR. Besides, the invention further provides an in-vitro expression method of the monopterus albus AKR gene.

A preparation technology for manufacturing methandienone by a microbe conversion method includes preparation of microorganism suspension liquid applying simple arthrobacterin, first stage seeds cultivation, second stage fermentation cultivation, substrate conversion and product separation and extraction to obtain the product methandienone meeting BP80 standard by coherent technology of cultivation period and conversion period and applying Girard reagent to separate the methandienone and a substrate specifically, the recovered substrate methyltestosterone can be converted again by said strains.

The invention discloses a nano-scale collagen purification method capable of reaching the absorption rate of 96%. The method includes the steps: crushing fish skins, and removing impurities; placing crushed materials into a low-temperature stirrer, stirring and mixing mixture for 30 minutes according to the volume ratio of the crushed materials to deionized water, standing for 10 minutes, removingupper floats, and placing mixture into a filter device to perform adsorption filtration; collecting filtered crushed materials, and performing first purification, second decomposition and third purification. The volume ratio of the crushed materials to deionized water is 1:3. Nano-scale collagen acquired through three purification steps and a particle collisions instrument is detected by SGS andPUNY, so that the nano-scale collagen is free from oestrone, chloramphenicol, progesterone, salmonella, methyltestosterone, macrodantin metabolite, fat, estradiol and estriol. According to the method,slurry acquired after treatment of the fish skins is used for extraction of the collagen, the purification rate and the purity of the collagen can be obviously increased, the slurry is purified by anano-scale collagen purification device to obtain nano-scale mixed powder, collision of the nano-scale mixed powder is implemented by the particle collisions instrument to obtain the nano-scale collagen with uniform molecular weight, and the absorption rate of the nano-scale collagen can reach 96%.

The invention provides a method for measuring sex hormone residue in cosmetics. An analysis method for simultaneously measuring pregnendione, testosterone propionate, medroxyprogesterone acetate, norgestrelm, hydroxyprogesterone acetate and methyltestosterone residue in the cosmetics through high-performance liquid chromatography-tandem mass spectrum is established. The method comprises the following steps: firstly pretreating cosmetic samples of different preparations, and taking acetonitrile and n-hexane as extraction solvents, ultrasonically extracting for 20min, and centrifuging a sample extracting solution for 10min in the speed of 12000r/min; selecting the acetonitrile and water as moving phases to perform isogradient elution, wherein the flow velocity is 0.3mL/min, and the column temperature is 25 DEG C, and performing separation by adopting an octadecyl silane chemically bonded silica chromatographic column. A result shows that a linear regression equation correlation coefficient R of each of six sex hormones is 0.9993-0.99989, the recycling rate is between 97.6%-99.5%, and the accuracy is between 0.5%-3.1%. The method provided by the invention is simple, sensitive, fast, reliable, good in reproducibility, and suitable for content analysis detection of the hormone addition of sex hormones in the cosmetics.

The invention discloses a colloidal gold detection card and a preparation method thereof, and a hormone residue detection method using the colloidal gold detection card. The colloidal gold detection card comprises a supporting plate, a sample pad, a colloidal gold combination pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad, the colloidal gold combination pad, thenitrocellulose membrane and the water absorption pad are sequentially arranged on the plate surface of the supporting plate in the direction from the first end to the second end of the detection card,and the nitrocellulose membrane is provided with a detection line and a quality control line; and the colloidal gold combination pad is coated with an anti-methyltestosterone monoclonal antibody andan anti-diethylstilbestrol monoclonal antibody which are marked by colloidal gold. According to the technical scheme, the residues of the androgen and the estrogen in the aquatic products can be detected at the same time, and therefore the detection efficiency is improved.

The invention provides a scheme capable of reducing damages caused by operations during ripening-accelerating processes as well as effectively and efficiently strengthening ovarian development of Japanese eels. The inventor provides an additive for promoting the ovarian development of the Japanese eels, wherein the additive comprises androstenedione and methyltestosterone, and the ratio of the androstenedione to the methyltestosterone in the additive for promoting the ovarian development of the Japanese eels is 4:6-6:4. The inventor further discloses a method for promoting the ovarian development of the Japanese eels by applying the additive in the breeding processes of the Japanese eels. The method is capable of effectively raising gonad indexes of the female Japanese eels. Moreover, the method and the additive are convenient to use; and no damage is caused to the Japanese eel broodstocks by carrying out the operations during the application processes.

The invention discloses a treatment method for methyltestosterone mother liquor. The treatment method comprises the following steps that 1, an ethyl acetate evaporation solvent is added into the methyltestosterone mother liquor, then, a mixed solvent, acetone cyanohydrin and organic aliphatic amine are added for reacting, and an intermediate material is obtained; 2, methyl alcohol is added into the intermediate material obtained in the first step, after uniform stirring, alkaline water is added for reacting, and crude 4-androstenedione is obtained; crude 4-androstenedione is refined, and refined 4-androstenedione is obtained through concentration, freezing and filtering; filter liquor obtained through filtering in the refining process of refined 4-androstenedione is subjected to concentration, freezing, filtering and recrystallization, and methyltestosterone is obtained. By means of the method, emissions of hormone waste are reduced, and pollution to the environment is reduced; 4-androstenedione and methyltestosterone can be recycled, recycled 4-androstenedione can be utilized again for methyltestosterone production, resource waste is avoided, and the production cost is greatly reduced.