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Preparation technique for producing mayandrosteron through microbial conversion method

A technology of microbial transformation and metandrone, applied in the direction of fermentation, etc., can solve the problems of complex operation, low feeding concentration, small scale, etc., and achieve the effect of simple operation process, slight environmental pollution, and increased yield of finished products

Inactive Publication Date: 2005-01-12
天津市福兴达怡保药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the preparation of dehydromethyltestosterone by microbial transformation method has related research at home and abroad, and even some reports can reach more than 80% yield, these studies are only in the laboratory stage, with small scale and complicated operation. And the feeding concentration is very low, generally no more than one thousandth
[0006] In summary, it is not difficult to see that the key issues that affect the popularization and application of biofermentation to produce methandrosterone are the concentration of feed, production scale, production cost and ease of operation.

Method used

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  • Preparation technique for producing mayandrosteron through microbial conversion method
  • Preparation technique for producing mayandrosteron through microbial conversion method
  • Preparation technique for producing mayandrosteron through microbial conversion method

Examples

Experimental program
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Effect test

Embodiment 1

[0044] The production strain is Arthrobacter simplex preserved in the Department of Applied Microbiology of Tianjin University of Science and Technology, numbered BY31250. The production strain was cultured on an agar slant at a constant temperature of 30°C for 2 days, and stored at 4°C for 5 days for use. Wherein, the liquid used for making the bacterial seed suspension is sterile water or fresh seed culture medium, and the sterilization condition is: 121° C. for 20 to 25 minutes.

[0045] The seed medium consists of carbon source, nitrogen source and inorganic phosphorus, namely:

[0046] Glucose 8g / L

[0047] Corn syrup 10g / L

[0048] Yeast extract 3g / L

[0049] K H 2 PO 4 1.5g / L

[0050] pH 6.4

[0051] The prepared seed suspension was added to the above-mentioned seed medium, and the sterilization condition was 121°C for 25 minutes; the shaker rotated at 60r / min, and cultured at 30°C for 22h to obtain a seed culture suitable for inoculation.

[00...

Embodiment 2

[0063] Use the fresh seed slant, that is, the production strain to be used directly after 2 days of constant temperature cultivation at 30°C on the agar slant, and prepare the bacterial suspension with sterile water. Described in one-level seed culture, secondary fermentation culture example 1. The substrate 17α-methyltestosterone (17α-methyltestosterone) was pulverized to a particle size of 14-16μ, and was quickly put into the cultured fermentation broth under aseptic conditions, and the feeding amount was 1.2g / L fermentation broth volume, and the relative In the industrial ethanol of 3% of fermented liquid volume, transformation condition is described in example 1. After 9 hours of transformation, 4% industrial ethanol relative to the volume of the fermentation broth was added; after 48 hours of transformation, samples were taken to control the end point by TLC and HPLC.

[0064] After the conversion, the extraction and separation of the fermented liquid are the same as des...

Embodiment 3

[0066] Use the fresh seed slant, that is, the production strain to be used directly after 2 days of constant temperature cultivation at 30°C on the agar slant, and prepare the bacterial suspension with sterile water. Described in one-level seed culture, secondary fermentation culture example 1.

[0067] Pulverize the recovered substrate with a particle size of 14-16 μ, quickly put it into the cultured fermentation broth under aseptic conditions, and the feeding amount is 2.0g / L fermentation broth volume, and add 3% industrial alcohol relative to the fermentation broth volume, The transformation conditions were as described in Example 1. After 8 hours of transformation, 4% industrial ethanol relative to the volume of the fermentation broth was added; after 24 hours of transformation, samples were taken to control the end point by TLC and HPLC.

[0068] After the conversion, the extraction and separation of the fermented liquid are the same as described in Example 1, and the co...

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Abstract

A preparation technology for manufacturing methandienone by a microbe conversion method includes preparation of microorganism suspension liquid applying simple arthrobacterin, first stage seeds cultivation, second stage fermentation cultivation, substrate conversion and product separation and extraction to obtain the product methandienone meeting BP80 standard by coherent technology of cultivation period and conversion period and applying Girard reagent to separate the methandienone and a substrate specifically, the recovered substrate methyltestosterone can be converted again by said strains.

Description

technical field [0001] The invention relates to a production method of anabolic hormone drugs, in particular to a preparation process for producing methandrosterone by a microbial conversion method. Background technique [0002] Methandienone is dehydro-17α-methyltestosterone (Dehydro-17α-methyltestosterone), chemical name 17β-hydroxy-17α-methylandroster-1,4-dien-3-one (17β-hydroxy- 17α-methylandrosta-1, 4-dien-3-one), commonly known as Dianabol (Dianabol), is a protein anabolic hormone, which can significantly promote protein synthesis (assimilation), reduce amino acid decomposition (dissimilation), and make muscle Growth, weight gain, reduce azotemia, and at the same time promote bone marrow hematopoietic function, mainly used for protein assimilation or insufficient absorption, and protein decomposition hyperactivity or excessive loss, such as severe burns, postoperative chronic wasting disease, old age Osteoporosis and tumor bad fluid juice and other patients. It has b...

Claims

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Application Information

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IPC IPC(8): C12P33/02
Inventor 杜连祥王致萍卢继坤王树立别松涛
Owner 天津市福兴达怡保药业有限公司
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