The invention discloses an adhesive for rubber footwear lasting and a preparation method of the adhesive. The adhesive comprises the following raw materials in parts by weight: 1# standard rubber, zinc oxide, stearic acid, an accelerant M, an accelerant D, an anti-aging agent D, light calcium carbonate, rosin, 1# standard rubber threads, 120# gasoline, a terpene resin, industrial alcohol and sulfur. The adhesive has high initial adhesion and adhesive properties and is high in cohesive strength, and even if the adhesion is ineffective under temperature conditions of a drying oven, a phenomenon that the lasting is loosened is difficultly caused. Meanwhile, the adhesive has the characteristics of low price, low toxicity, quick dryness and simplicity in manufacture. In the preparation method, high molecular weight of natural rubber is kept, and the rubber footwear lasting is difficultly loosened under the drying conditions after being adhered. Moreover, the condition that the initial adhesion of the high molecular weight raw rubber threads does not meet the adhesion requirement is improved by directly adding a tackifier terpene resin into an adhesive cement pot, the industrial alcohol is added to reduce the viscosity, the brushing properties of the high molecular weight rubber threads are improved, and the raw foam rubber vulcanization speed is increased.