730 results about "Pathogenic fungus" patented technology

The invention relates to bacillus velezensis SMB1 and application thereof in prevention and treatment of plant diseases, and belongs to the technical field of biological prevention and treatment of plant diseases. The strain is preserved in the China Center for Type Culture Collection on November 27, 2024, the preservation address is Luojia mountain, Luojia mountain, eight road, Wuchang District, Wuhan City, Hubei Province, and the preservation number is CCTCC NO: M20242647. The bacillus velezensis SMB1 provided by the invention has a remarkable bacteriostatic effect on plant pathogenic fungi, plant pathogenic oomycetes, plant pathogenic bacteria and animal pathogenic bacteria, has an extremely good broad-spectrum bacteriostatic function, has a great application potential in biological control of plant diseases, can reduce the use of chemical pesticides, and has a good application prospect. The pesticide residue in agricultural products is reduced, the environment is protected, and the urgent demand on pollution-free agricultural product production at present is met.

The invention discloses bacillus velezensis and application of the bacillus velezensis in prevention and treatment of basal stem rot, the classification name of the bacillus velezensis is bacillus velezensis GN-22, the bacillus velezensis is preserved in China Center for Type Culture Collection (CCTCC), the preservation number of the bacillus velezensis is CCTCC NO: M2025566, and the preservation date is March 24, 2025. The bacillus velezensis GN-22 disclosed by the invention belongs to rhizosphere growth-promoting bacteria, has a remarkable inhibition effect on different pathogenic fungi such as fusarium graminearum, fusarium pseudograminearum, fusarium asiaticum, fusarium verticillioides and fusarium oxysporum, and plays an important role in biological control of diseases such as corn and wheat stem rot; meanwhile, the strain has a growth promoting effect and can promote corn growth. The strain provided by the invention has the advantages of simple culture conditions, easiness in preservation, easiness in industrial production and good development and application prospects.

The invention discloses a paenibacillus kribbensis spaceflight mutant strain and application of the paenibacillus kribbensis spaceflight mutant strain in preparation of nano-selenium. According to the invention, a paenibacillus kribbensis PS04-2 strain is obtained through space mutation, and the strain has high selenite reducing capacity and can tolerate high-concentration selenite, and the tolerable concentration exceeds 1000mM; the method has the capacity of efficiently converting sodium selenite to generate nano-selenium, the synthesized nano-selenium is high in conversion rate and uniform in nano-selenium particle, and the provided preparation method is simple, convenient, low in cost, high in product purity and environmentally friendly. The nano-selenium synthesized by the PS04-2 strain has a good antagonistic effect on various plant pathogenic fungi, can prevent and treat various plant diseases, and can be used for developing more selenium-enriched microbial preparations. Meanwhile, nano-selenium synthesized by the PS04-2 strain is used for sesame production, so that sesame growth can be promoted, selenium-rich sesame can be obtained, and more effective methods are provided for increasing the selenium content of crops.

The invention relates to the technical field of microorganisms, and particularly discloses a saline-alkaline tolerant bacillus deserticola BW-13 with biocontrol and growth promoting functions and application thereof. The bacillus safensis BW-13 is preserved in the China Center for Type Culture Collection on January 13, 2025, the classification name of the bacillus safensis BW-13 is bacillus safeensis, and the preservation number of the bacillus safensis BW-13 is CCTCC NO: M 2025108. The strain BW-13 has relatively high salt tolerance, and still keeps good resistance under various stress conditions; meanwhile, the plant growth promoting agent has various plant growth promoting characteristics including nitrogen fixation, phosphorus solubilization and cellulase and protease secretion capacity, and pot experiment verifies that the plant growth promoting agent can improve the salt resistance of cucumber seedlings and promote plant growth; the strain has an antagonistic effect on a plurality of phytopathogens, and especially has a remarkable prevention and treatment effect on plant diseases such as apple ring spot, polygonatum kingianum anthracnose, pear tree rot and tomato gray mold caused by ascomycophyta pathogenic fungi.

The invention discloses bacillus amyloliquefaciens GX0003936 and application thereof, and belongs to the technical field of microorganism application. The invention discloses bacillus amyloliquefaciens GX0003936 with a broad-spectrum bacteriostatic effect, which is preserved in the China General Microbiological Culture Collection Center on March 26, 2025, and the preservation number of the bacillus amyloliquefaciens GX0003936 is CGMCC (China General Microbiological Culture Collection Center) No.33987. The bacterial strain has broad-spectrum antibacterial activity, can be used for remarkably inhibiting the growth of mycelia of colletotrichum musae and colletotrichum frutescens, and can be used for remarkably reducing the disease incidence rate of tropical fruits such as bananas, mangoes and the like in the postharvest storage period. Meanwhile, the strain shows a good inhibition effect on various plant pathogenic fungi, and has a potential broad-spectrum biocontrol effect.

The invention discloses bacillus velezensis XF-8 and application of the bacillus velezensis XF-8 in prevention and treatment of tomato bacterial wilt caused by Ralstonia solanacearum, and belongs to the technical field of microorganisms. The bacillus velezensis XF-8 capable of antagonizing Ralstonia solanacearum is obtained through separation and screening, and it is found that the bacillus velezensis XF-8 also has an excellent inhibition effect on various pathogenic fungi and shows broad-spectrum antibacterial activity of the bacillus velezensis XF-8. The bacillus velezensis XF-8 has a good inhibition effect on tomato bacterial wilt, and the control effect of the bacillus velezensis XF-8 in potted tomatoes reaches 74.25%. Results of the invention show that the bacillus velezensis XF-8 has a great application prospect in prevention and treatment of tomato bacterial wilt, and a good biocontrol resource is provided for research and development of a biocontrol agent for tomato bacterial wilt.

The invention provides a bacillus velezensis strain and application thereof. The name of the bacillus velezensis is BJ-1141, and the bacillus velezensis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.32094. The strain has broad-spectrum antibacterial activity, and has a good inhibition effect on 24 plant pathogenic fungi such as rice pathogenic bacteria, wheat pathogenic bacteria, corn pathogenic bacteria, grape pathogenic bacteria, cherry pathogenic bacteria, solanaceous crop pathogenic bacteria and the like. Wherein the growth inhibition effect on sclerotinia sclerotiorum hyphae causing eggplant sclerotinia rot is optimal, and the inhibition rate can reach 95.57%. The BJ-1141 bacterial liquid has an obvious growth promoting effect on the growth of plants such as tomatoes and lettuce. After the BJ-1141 bacterial liquid is used for irrigating roots of potted tomatoes, the tomato fusarium wilt caused by tomato fusarium oxysporum can be effectively controlled, the BJ-1141 bacterial liquid has a good prevention and treatment effect, the prevention and treatment effect can reach 85.93%, and the prevention and treatment effect is obviously different from that of a control group.

The invention provides application of a pseudomonas parabarium YC15 strain in efficient prevention and treatment of wheat stem rot and various pathogenic bacteria. The invention provides a new application of the pseudomonas parabarium YC15 strain in prevention and control of plant diseases, and research shows that the YC15 strain has broad-spectrum bacteriostatic activity, has remarkable inhibitory activity on various plant pathogenic bacteria, can inhibit pathogenic fungi such as wheat stem rot, sesame root rot and colletotrichum gloeosporioides, and has a wide application prospect in prevention and control of plant diseases. And the compound has a relatively good inhibition effect on pathogenic bacteria, namely sesames pseudomonas solanacearum. The YC15 strain is applied to prevention and control of wheat stem rot, and pot experiments show that after the YC15 strain is adopted for treatment, plant lesions can be reduced, the inhibition effect of pathogenic bacteria on plants can be broken, the pathogenic bacteria can be effectively inhibited, plant growth can be promoted, and the wheat stem rot can be efficiently prevented and controlled. The invention provides new application of the YC15 strain, and the YC15 strain can be used for preventing and treating various plant protobacteria and diseases thereof, and has good development and application potential as a biocontrol bacterium.

The invention discloses paenibacillus polymyxa K17 for preventing and treating diseases, the preservation number of the paenibacillus polymyxa K17 is CGMCC (China General Microbiological Culture Collection Center) No.33366, and the paenibacillus polymyxa K17 is classified and named as Paenibacillus polymyxa K17. The strain has a good anti-antagonism effect on 16 different plant pathogenic fungi from 8 crops; particularly, the antibacterial effect on various leaf spot diseases of tea trees is superior to that of part of common biocontrol strains such as Pristeria aryabhattai 11, Bacillus tequilensis R2 and the like. The control effect on tea tree leaf spot and tea tree anthracnose is higher than 70%, the control effect on black spot of picked tomatoes is 68%, the control effect is equivalent to that of various chemical pesticides, and the method has extremely high application prospects and market value in field biological control and postharvest preservation of plant diseases. Meanwhile, the strain K17 has the plant growth-promoting characteristics of secreting multiple extracellular hydrolases, dissolving inorganic phosphorus and mineral potassium and biologically fixing nitrogen, and has the potential of preparing plant growth-promoting microbial agents or bacterial fertilizers.

The invention belongs to the technical field of microbiology and agricultural application thereof, and particularly relates to bacillus aerosus, a microbial agent and application of the microbial agent. According to the invention, a strain with good antagonism on tobacco black shank and fusarium root rot is separated and screened from tomato roots, and is identified as aerobacillus, and chitinase, cellulase and protease are secreted to dissolve the cell wall structure of pathogenic bacteria hyphae and inhibit the growth of pathogenic bacteria, so that the pathogenic bacteria lose the infection ability, and the pathogenic bacteria are effectively prevented from being infected. The biocontrol microbial inoculant has the antibacterial effect on more than 10 plant pathogenic fungi, the antibacterial rate is greater than 80%, especially the antibacterial rate on phytophthora nicotianae and fusarium oxysporum is greater than 90%, and pot experiments and field experiments prove that the biocontrol microbial inoculant has the control effect on tobacco black shank and fusarium root rot obviously superior to those of commercially available biocontrol microbial inoculants and chemical agents; meanwhile, the tobacco seed germination can be promoted, the growth and development of field tobacco plants are promoted, the subculture stability is better, and the application prospect is wide.

The invention relates to the technical field of biocontrol strains for crops, in particular to bacillus velezensis JR1A and application thereof to inhibition of garlic pathogenic fungi. The bacillus velezensis JR1A is preserved in the China General Microbiological Culture Collection Center on June 30, 2025, and the preservation number of the bacillus velezensis JR1A is CGMCC No.35059. The bacillus velezensis JR1A is named as bacillus velezensis JR1A. The JR1A inhibits mycelial growth and spore production of pathogenic fungi of common garlic diseases, has an excellent biocontrol effect and a certain growth promoting function, can reduce the relative abundance of pathogenic fungi in garlic crop rotation soil, and reduces the occurrence of related garlic diseases.

The invention belongs to the technical field of microorganisms, and particularly relates to a Prussia adamsii T54 strain, a microbial agent and application of the Prussia adamsii T54 strain and the microbial agent. The Pichia aryabhattai T54 is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms on February 21, 2025, and the preservation number of the Pichia aryabhattai T54 is CGMCC (China General Microbiological Culture Collection Center) No. 33613. According to the present invention, the Princisteria aryabhattai T54 and the bacterial agent have significant inhibition effects on a variety of plant pathogenic fungi, and further have plant growth promoting effects;

The invention provides a trichoderma Guizhou B10 strain and application thereof, and relates to the technical field of agricultural microbial control. The trichoderma Guizhou B10 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No. 3.25634. The invention overcomes the defects in the prior art, and the strain can inhibit pathogenic fungi of malania oleifera leaf spot disease, such as pestalotiopsis, pestalotiopsis, clostridium neocarpum, botryosphaeria and physophaga fungi, can promote the growth of malania oleifera plants, and has both disease-resistant and growth-promoting functions on malania oleifera seedlings, such as malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, and malania oleifera leaf spot disease resistance.

The invention discloses application of phloroglucinol compounds to inhibition of fusarium plant pathogenic fungi and prevention and treatment of related plant diseases, and belongs to the technical field of agricultural bactericides. In order to solve the technical problems that an existing method for preventing and treating plant diseases caused by fusarium is poor in effect and has defects, 15 compounds are extracted from dryopteris fragrans, and structural identification shows that the 15 compounds belong to phloroglucinol compounds. Then, the in-vitro bacteriostatic effects of the 15 compounds on fusarium oxysporum, fusarium graminearum and fusarium laminatum are detected, the compounds 5, 6, 7, 8, 10, 11, 13 and 14 are found to have bacteriostatic effects on fusarium fungi, and the compound 10 (aspidinol B) has the strongest in-vitro inhibitory effect on the three fusarium fungi; furthermore, an in-vivo antibacterial experiment proves that the compound 10 has a good prevention and treatment effect on pathogenic fungal diseases of three fusarium plants.

The invention discloses paenibacillus sp. H31, which is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M 20252669, and the preservation date is November 24, 2025. The invention further discloses a preparation method of the paenibacillus sp. The paenibacillus H31 disclosed by the invention not only has broad-spectrum disease-resistant activity on pathogenic fungi of various plants, but also has a remarkable growth-promoting effect on pepper, so that the paenibacillus H31 can be widely applied to prevention and treatment of agricultural diseases and insect pests, and sustainable development of green agricultural production is promoted. In addition, the paenibacillus H31 also has the effects of hydrolyzing proteinase and cellulose and decomposing ferritin, so that the paenibacillus H31 also can be applied to the scenes of industrial production and the like.

The invention discloses a bacillus velezensis strain and an application thereof in agriculture. The bacillus velezensis QK-4 is obtained through screening, the bacterial strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacterial strain is CGMCC NO.32225. The bacillus velezensis QK-4 is a bacillus velezensis QK-4 strain, and the preservation number of the bacillus velezensis QK-4 strain is CGMCC NO.32225. The bacillus velezensis QK-4 strain is a bacillus velezensis QK-4 strain. The bacillus velezensis disclosed by the invention has relatively strong saline-alkaline resistance, can keep relatively high activity in a saline-alkaline environment, and can effectively convert phosphorus in a fixed state of soil into phosphorus which can be utilized by plants, so that the phosphorus nutritional condition of the soil is improved, the phosphorus utilization rate of crops is increased, the salinization of the soil is improved, and the soil quality is improved. Growth of crops in saline-alkali soil is promoted and crop yield is increased. Meanwhile, the strain has an obvious inhibition effect on diseases caused by various pathogenic fungi and can be used for preventing and treating various plant diseases.

The invention discloses a salt-tolerant brevibacillus for promoting growth and preventing and controlling plant diseases and application of the salt-tolerant brevibacillus. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025, and the preservation number of the brevibacillus halotolerans T101 is GDMCC No: 66731. The brevibacillus halotolerans T101 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 23, 2025. The strain T101 disclosed by the invention can effectively antagonize plant pathogenic fungi peronophythora litchii and plant pathogenic bacteria such as pseudomonas syringae, xanthomonas campestris, citrus canker pathogen and ralstonia solanacearum, can generate siderophores and plant growth hormone IAA, and can remarkably promote the growth of tomato root systems in a pot experiment. Therefore, the strain T101 can be used for preparing microbial preparation products for preventing and treating plant diseases and promoting plant root growth, and has important application potential in the aspects of microbial agents, biological fertilizers and the like for promoting plant growth and development.

A mixture containing lipopeptide compounds is separated from bacillus velezensis TCS001 fermentation liquor, and it is found that the mixture containing the lipopeptide compounds and the five lipopeptide compounds have the activity of inhibiting plant pathogenic fungi and are more stable compared with the bacillus velezensis TCS001. And the method has wide application value in field disease prevention and control. By optimizing the formula of the bacillus velezensis TCS001 culture medium and the culture and fermentation process conditions, the yield and yield of lipopeptide compounds in the bacillus velezensis TCS001 fermentation liquor are increased.

The invention belongs to the technical field of microorganisms, and discloses streptomycete Y309 and application thereof. The preservation number of the streptomyces sp. Y309 is GDMCC No: 64527, and the streptomyces sp. Y309 has relatively strong secondary metabolite synthesis capability and can be used for producing siderophore, IAA (Indoleacetic Acid) and the like. The strain Y309 also has the potential of plant growth promotion and disease control, and the strain Y309 has a relatively strong bacteriostatic effect on 12 pathogenic fungi such as fusarium oxysporum, rhizoctonia cerealis, monilinia fusarium and coracocystis fimbristipula as well as 7 pathogenic bacteria such as pseudomonas syringae lacrimation pathogenic variants and bacillus cereus, and has a relatively strong bacteriostatic effect on 12 pathogenic fungi such as fusarium oxysporum, rhizoctonia cerealis, monilinia fusarium and coracocystis fimbristipula. The strain Y309 has good prevention and treatment effects on wheat sharp eyespot, tomato gray mold and pepper fusarium wilt, fermentation liquor of the strain Y309 can remarkably promote wheat seed germination and seedling growth, agronomic characters such as the plant height, the dry weight and the leaf area in the corn seedling stage can be improved in different saline-alkali soil, and the strain Y309 has good application prospects.

The invention provides a drug target protein MoFrd1 and application of the drug target protein MoFrd1 in agricultural disease control. The amino acid sequence of the MoFrd1 is as shown in SEQ ID No. 1, and the nucleotide sequence of the coding gene of the MoFrd1 is as shown in SEQ ID No. 4. Researches show that the pathogenicity of the magnaporthe oryzae can be remarkably reduced by knocking out the MoFrd1 coding gene. Homologous protein of MoFrd1 widely exists in plant pathogenic fungi and shows high conservative property in pathogenic mechanisms, however, homologous protein is not found in plants and mammals, and it is indicated that the homologous protein of MoFrd1 has the potential of serving as a specific drug target of pathogenic bacteria. Based on a key structural domain and a functional site of the MoFrd1 protein, a specific agent for preventing and treating plant fungal pathogens is expected to be developed through design or screening. The invention provides a new drug target and a control strategy for controlling fungal diseases of crops, and has important application value and market potential.

The invention relates to the field of microorganisms, and provides bacillus velezensis 20-1 and application thereof, the bacillus velezensis 20-1 is preserved in the China Center for Type Culture Collection on July 26, 2024, and the preservation number is CCTCC NO: M 20241694. The bacillus velezensis 20-1 can effectively inhibit the growth of common aquatic pathogenic fungi, has high thermal stability and protease tolerance, and can be used for preventing, relieving and / or treating diseases caused by aquatic pathogenic fungi infection.

The invention discloses a bacterial strain for antagonizing multiple pathogenic fungi of potatoes and simultaneously promoting growth and application of the bacterial strain, and relates to the field of microbial agents, the bacterial strain is bacillus wiedmannii Bw34, the bacterial strain is preserved in China General Microbiological Culture Collection Center, the preservation address is No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is November 11, 2024, and the preservation number is CGMCC NO. The preservation number is CGMCC (China General Microbiological Culture Collection Center) The compound is used for preparing a fungicide for antagonizing potato pathogenic fungi and a fungicide for promoting potato growth. The Bw34 has the effect of comprehensively preventing and treating potato soil-borne diseases, meanwhile, potato growth and nutrient absorption are promoted, and the application prospect is good.

The invention belongs to the technical field of biological control, and particularly discloses bacillus velezensis and application of the bacillus velezensis to preparation of a biocontrol agent and a composite agent for controlling tobacco fusarium root rot, the strain is bacillus velezensis N4, is preserved in China General Microbiological Culture Collection Center on September 23, 2024, and has the preservation number of CGMCC NO. The preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.32040, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The bacillus velezensis N4 provided by the invention has the capability of efficiently antagonizing tobacco root rot fungi F. tobactrum sp. Nov., and has an obvious antibacterial effect on a plurality of plant pathogenic fungi; after the bacillus velezensis N4 wettable powder disclosed by the invention is combined with a chemical agent propiconazole in the field, the bacillus velezensis N4 wettable powder has a good control effect on tobacco root rot and can promote the growth of tobacco plants, and the control effect is improved while the application of chemical pesticides is reduced.

The invention discloses bacillus tequilensis 83IBC04-2 and application thereof, the bacillus tequilensis 83IBC04-2 comes from a deep sea hydrothermal zone and is preserved in China Center for Type Culture Collection on October 15, 2025, and the preservation number is CCTCC NO: M20252212. The compound has a remarkable antagonistic effect on a plurality of important pathogenic fungi such as tomato gray mold, tomato early blight, rice blast and wheat scab, can be used for preparing a broad-spectrum and high-efficiency biological bactericide, effectively reduces the use amount of chemical pesticides, and reduces pesticide residues and environmental pollution.

The invention discloses Pseudomonas aeruginosa KS1-2 and application thereof, the strain is separated from a soil sample in a rice field and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.33996. The invention further discloses a method for preparing the Pseudomonas aeruginosa KS1-2. The Pseudomonas aeruginosa KS1-2 has the advantages that the Pseudomonas aeruginosa KS1-2 can be used for preparing the Pseudomonas aeruginosa. The pseudomonas aeruginosa KS1-2 has a remarkable inhibition effect on a plurality of rice pathogenic bacteria; the growth of various plant pathogenic fungi can be effectively inhibited, and the broad-spectrum antibacterial property is achieved; the strain and the microbial inoculum can be prepared into a biocontrol agent for preventing and treating plant pathogens, can reduce plant diseases, are green and environment-friendly, have good application potential, and lay a foundation for research and development of multifunctional biocontrol microbial inoculum and biopesticide.

The invention relates to a nucleic acid combination product, a kit for detecting pathogenic fungi and a detection method. The nucleic acid combination product provided by the invention comprises a composition of a plurality of groups of primer pairs and detection probes, is wide in detection coverage and high in inclusiveness, can simultaneously detect a plurality of pathogenic fungi in a single-tube reaction system, has relatively high sensitivity and specificity, is a pathogenic fungi detection product with relatively comprehensive detection targets at present, and has a wide application prospect. And an auxiliary diagnosis basis is provided for clinical examination.

The invention discloses a novel application of massinia phosphate solubilizing C22 in prevention and treatment of fungal diseases of plants, and belongs to the technical field of agricultural biology, and particularly relates to a novel application of massinia phosphate solubilizing C22 in prevention and treatment of fungal diseases of plants. The known strain C22 has a remarkable antagonistic effect on pathogenic bacteria fusarium pseudograminearum of wheat stem rot, and the plate inhibition rate reaches 36.79%. Potting and field tests prove that wheat basal stem rot can be effectively prevented and treated by applying the C22 fungicide, the morbidity in the seedling stage is reduced by 21.64%, the morbidity in the adult-plant stage is reduced by 55.06%, the DON toxin content in plants can be remarkably reduced by 68.26%, and the pathogenic bacterium load can be remarkably reduced. According to the invention, the potential of the phosphorus-solubilizing massinia sp. C22 in the aspect of biological control is disclosed for the first time, and a new core microbial resource and a technical scheme are provided for developing a green biological pesticide for controlling wheat stem rot.

The invention discloses bacillus velezensis ZF520 and a control effect of the bacillus velezensis ZF520 on cowpea root rot. The invention belongs to the field of microorganisms, and particularly relates to bacillus velezensis ZF520 and a control effect of the bacillus velezensis ZF520 on cowpea root rot. The bacillus velezensis disclosed by the invention is bacillus velezensis ZF520, and the preservation number of the bacillus velezensis is CGMCC (China General Microbiological Culture Collection Center) No.31235 in the China General Microbiological Culture Collection Center. The strain ZF520 has an inhibition effect on a plurality of pathogenic fungi, has broad-spectrum bacteriostatic activity, has a cowpea root rot potting control effect of 83.59%, and has a cowpea root rot control effect of 73.99% by seed coating. The research provides an efficient and safe biocontrol resource for biological control of cowpea root rot.

The present disclosure relates to Bacillus velezensis (B. velezensis) and a use thereof, and belongs to the technical field of microorganisms. The present disclosure solves one of the problems of the Bacillus in the art such as poor broad-spectrum activity for fungi and bacteria, low inhibitory activity, and limited inhibition range. The B. velezensis QMHF-G5 has an excellent inhibitory activity for pathogenic fungi and bacteria, and can inhibit a variety of pathogenic fungi and bacteria, with a prominent broad-spectrum activity. In addition, the B. velezensis has a high inhibitory activity for a variety of pathogenic fungi and bacteria detected, with a high inhibition rate.