Plants endogenetic fungus and application thereof

A technology of plant diseases and germs, applied in the field of plant endophytic fungi and its application, can solve the problem of few studies on tolerance and achieve good inhibitory effect

Inactive Publication Date: 2008-08-06
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research on the production of volatile natural gas compounds by grass endophytic fungi has not been reported at home and abroad...

Method used

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  • Plants endogenetic fungus and application thereof
  • Plants endogenetic fungus and application thereof
  • Plants endogenetic fungus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. Screening and identification of Brachiaria endophytic fungal strain Acremonium strictum HND5 CGMCCNo.2192

[0032] 1. Screening of the Brachiaria endophytic fungus strain Acremonium strictum HND5 CGMCCNo.2192

[0033] The endophytic fungus strain Acremonium strictum (Acremonium strictum) HND5CGMCC No.2192 of the present invention is isolated from healthy leaves of Brachiaria sinensis by adopting a strict surface disinfection procedure using a tissue block separation method. The specific method is as follows: the surface of fresh and healthy Brachiaria saponifera blades is disinfected, washed with sterile water for 4 times, and then the excess water on the blades is blotted with sterile filter paper. Cut the leaves into pieces with sterile scissors, and inoculate them on a PDA plate (recipe: 200 g of peeled potatoes, 20 g of glucose, 20 g of agar, boil the peeled potatoes for 30 min, dilute the filtrate to 1000 mL with distilled water, sterilize for 20 min at ...

Embodiment 2

[0041] Example 2, identification of antagonistic activity of endophytic fungal strain Acremonium strictum (Acremonium strictum) HND5 CGMCCNo.2192 to pathogenic fungi

[0042] Detection of the antagonistic activity of Acremonium strictum (Acremonium strictum) HND5 CGMCC No.2192 against pathogenic fungi, including against mango anthracnose, banana wilt, banana anthracnose, rubber anthracnose, rubber spores, and papaya Antagonistic activities against pathogens, Fusarium wilt of bamboo, Magnaporthe oryzae, Flax spot fungus, Sugarcane red rot fungus and Brachiaria leaf spot fungus.

[0043] The pathogenic fungi for testing are: mango anthracnose fungus (Colletotrichum gloeosporioides, purchased from China Agricultural Microorganism Strain Preservation Management Center, preservation number ACCC No.31219), banana wilt fungus (Fusariumoxysporium f.sp.cubense, purchased from China Agricultural Microorganism Strain Preservation Management Center) Center, preservation number ACCC No.312...

Embodiment 3

[0049] Embodiment 3, research on the basic biological characteristics of Acremonium strictum (Acremonium strictum) HND5 CGMCC No.2192

[0050] 1. Determination of growth curve of Acremonium strictum HND5 CGMCC No.2192

[0051] Use a sterilized hole puncher to get a piece of Acremonium strictum (Acremonium strictum) HND5 CGMCC No.2192 bacterial piece with a bacterial age of 2 weeks (cultivated on a PDA medium plate) and a diameter of about 4 mm into 50 mL of liquid PDA. 28°C, 180rpm shaking culture. Take the bacterial liquid from the 1st day to the 7th day, the 9th day and the 15th day respectively, collect the bacterial cells after vacuum pumping the filtrate, and weigh their wet weight and dry weight respectively (dry at 60°C to constant weight) (g). It was found that the peak of dry and wet heavy bacteria appeared on the 7th day, and then its biomass decreased; its growth curve is shown in Figure 4.

[0052] 2. Determination of the optimum growth medium for Acremonium str...

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Abstract

The invention discloses a method for producing fungi in a strain of plant and the application. Fungi which is produced in plants is Acremonium strictum HND5 CGMCC NO.2192. The bacterial strain and metabolite thereof can inhibit pathogen fungi activity in broad spectrum, can produce activity volatility natural gas chemical compound which can inhibit the activity of pathogenic fungi, the bacterial strain can parasitize banana root-knot nematode, metabolite can poison banana root-knot nematode, the bacterial strain can be widely used in the aspects of biologically preventing and curing plant diseases and pests, arm-shaped grass production, and in the aspects of fungi modified and breeding material.

Description

technical field [0001] The present invention relates to a plant endophytic fungus and its application, in particular to a plant endophytic fungus isolated from the leaves of Brachiaria saponifera and its use in inhibiting plant pathogenic fungi, parasitic nematodes and producing volatile natural gas compounds Applications. Background technique [0002] Plant endophytic fungi refer to some fungi that live in plants throughout their life cycle or most of their life history, and they have a mutualistic symbiotic relationship with their host plants. Endophytic fungi are provided by host plants with living space and nutrients, and their biomass is small, but they play an important role in the survival of host plants, can promote the growth of host plants, and enhance their disease resistance, insect resistance, and drought resistance. sex and growth competitiveness. Some endophytic fungi can form mycorrhizae together with the host root tissue, help the host plant absorb water a...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00A01P5/00
Inventor 黄贵修郭志凯蔡吉苗时涛刘先宝
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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