114 results about "Leaf spot" patented technology

The invention provides a moniliella malayi and a fungicide and application thereof, and relates to the field of microorganisms. The acer rubrum is specifically acer rubrum RA, and the preservation number of the acer rubrum RA is CGMCC (China General Microbiological Culture Collection Center) No.35889. The acer rubrum RA can be used for preparing the acer rubrum. The strain has the capability of decomposing inorganic phosphorus, is wide in phosphorus-dissolving range, can decompose tricalcium phosphate, iron phosphate and aluminum phosphate, and particularly still has very strong capability of decomposing inorganic phosphorus under a low-temperature condition; the strain has the capability of producing siderophore and IAA at low temperature; the strain has an inhibition effect on various pathogenic bacteria causing field crop diseases, and can inhibit diseases such as corn root rot, sorghum leaf spot or wheat root rot; the strain can be colonized in a soil environment of 5-40 DEG C, a microbial inoculum prepared from the strain can promote crop growth, the crop yield is remarkably improved, and high yield can be achieved even if the usage amount of phosphate fertilizer is reduced.

The invention discloses bacillus subtilis S1703 and application thereof, and belongs to the technical field of plant disease and pest control. The name of the bacterial strain is bacillus subtilis S1703, and the preservation number of the bacterial strain is GDMCC No: 66890. The bacterial strain S1703 has a wider antibacterial spectrum, and can effectively inhibit the growth of pathogenic bacteria such as fusarium oxysporum, colletotrichum gloeosporioides, alternaria alternata, aspergillus, phyllosticta, citrus reticulata, fusarium and the like; fermentation liquor of the S1703 has a good antibacterial effect on the psidium guajava wilt pathogen and the psidium guajava branch blight pathogen; the bacteriostasis rate on sigatoka sigatoka and guava leaf spot germs reaches 95% or above, and an excellent bacteriostasis effect is achieved. The strain S1703 can avoid potential adverse effects of chemical pesticides on the environment and crop safety, and has potential commercial development and application values in biological prevention and control practice of diseases.

This invention discloses a class of thiadiazole-benzohexacyclic compounds, their preparation methods, and applications. The structural formula is shown in Formula I. This invention introduces disulfide bond fragments into the thiadiazole-benzohexacyclic skeleton to obtain a novel class of thiadiazole-benzohexacyclic compounds. These compounds have a novel structure, a simple preparation method, readily available raw materials, and are unlikely to produce harmful byproducts. The compounds exhibit inhibitory effects against various plant pathogenic bacteria, particularly against *Citrus canker*, *Ralstonia solanacearum*, and *Bacterial angular leaf spot* of cucumber, demonstrating significant inhibitory effects. They can be used as agricultural bactericides and have great application value.

The present application belongs to the technical field of plant disease prevention and treatment, and particularly relates to a biocontrol bacterium for preventing and treating Guaiacum officinale anthracnose, a fermentation liquor, a biocontrol bacterium agent and application. Bacillus velezensis A biocontrol bacterium is isolated and screened from a microbial resource on a healthy Guaiacum officinale leaf, and is classified and named as Bacillus velezensis , which is preserved in the China General Microbiological Culture Collection Center on March 28, 2025, and has a preservation number of CGMCC No.34038; the biocontrol bacterium provided in the present application has good antagonistic effects on Colletotrichum gloeosporioides causing Guaiacum officinale anthracnose, on Stemphylium botryosum causing Guaiacum officinale leaf spot disease, on Botryosphaeria dothidea, on Alternaria alternata and on Pestalotiopsis versicolor capable of causing plant diseases. The biocontrol bacterium, the fermentation liquor and the biocontrol bacterium agent provided in the present application have potential application prospects in preventing and treating Guaiacum officinale anthracnose.

The application discloses a bacillus velezensis A13 wettable powder and application thereof, and belongs to the technical field of biological pesticides. The application aims to solve the problems of limited prevention and treatment effect of existing seed treatment, high labor intensity of foliar spraying and short effective period. The wettable powder comprises a mother powder prepared from fermentation liquor and diatomite, and a dispersant, a wetting agent, a stabilizer and a protective agent, wherein the total addition amount of the dispersant and the wetting agent is 5%-14% of the mass of the mother powder, the addition amount of the protective agent is 0.5%-2% of the mass of the mother powder, and the addition amount of the stabilizer is 1%-3% of the mass of the mother powder. The application can be used for preventing and treating root diseases caused by panax quinquefolius rust rot fungus and leaf diseases caused by panax quinquefolius alternaria leaf spot fungus, and promoting plant growth.

This invention relates to a method for detecting peanut leaf spot disease images in the canopy, overcoming the shortcomings of existing technologies in detecting peanut leaf spot disease in complex backgrounds. The invention includes the following steps: constructing a dataset; constructing a leaf spot disease detection model; training the leaf spot disease detection model; collecting and preprocessing high-resolution images of the peanut canopy to be detected; and obtaining the leaf spot disease detection results. This invention can handle the detection of small-target peanut leaf spot in complex backgrounds, balancing detection accuracy and real-time performance in the small-target detection problem, achieving real-time field-scale detection of peanut leaf spot disease in the canopy.

This invention relates to the field of microbial technology, specifically to a strain of *Bacillus laterosporus* and its applications. The *Bacillus laterosporus* provided by this invention (… Brevibacillus laterosporus HNBR05-1 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 25886. *Bacillus laterosporus* HNBR05-1 exhibits highly effective inhibition of plant pathogens, demonstrating dual efficacy against plant diseases such as brown spot and bacterial leaf spot, with high control efficacy. It shows promising application prospects in plant disease control and lays the foundation for the development of highly effective biocontrol agents.

The present application relates to a kind of oligonucleotide primer probe compositions for detecting Pseudomonas syringae, the pathogen of vegetable leaf spot disease based on ERA fluorescence method or ERA test strip method, and the detection method using the composition.The present application can simply, quickly, specifically, sensitively and visually broad-spectrum identify a variety of pathogenic variants of Pseudomonas syringae, the pathogen of vegetable leaf spot disease, the method is easy to operate, reaction time is short, without complex temperature control equipment, can be widely applied to the on-site rapid screening and disease diagnosis of Pseudomonas syringae in vegetable sample.

This invention belongs to the field of biological control technology for plant diseases and pests, and provides a strain of Chaetomium globosum ( Chaetomium globosum EF-14 and its applications. This strain is deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 40618. The *Chaetomium globosum* EF-14 provided by this invention is an endophytic fungus of maize that can simultaneously inhibit *Fusarium graminearum* (…). Fusarium gramineae ) and Corn spores ( Bipolaris maydis It can effectively reduce corn stalk rot and corn leaf spot caused by fall armyworm ( ) and can also effectively reduce the incidence of fall armyworm ( ). Spodoptera frugiperda This invention provides a highly efficient and safe microbial resource for the green control of major corn diseases and pests, and broadens the application scope of Chaetomium globosum in agricultural biocontrol, achieving synergistic control of diseases and pests through "one fungus targeting multiple targets".

The invention relates to a complex microbial inoculant and application thereof in prevention and control of tobacco black shank and tomato spotted wilt, and belongs to the technical field of prevention and control of tobacco diseases. The complex microbial inoculant comprises antibacterial lipopeptide and bacillus subtilis, wherein the mass ratio of the antibacterial lipopeptide to the bacillus subtilis is (0.5-1.2): 10; 0.08 wt% to 0.12 wt% of antibacterial lipopeptide powder is adopted as the antibacterial lipopeptide; the antibacterial lipopeptide comprises at least one of surfactin, iturin and abinogen; the viable count of the bacillus subtilis is greater than or equal to 2 * 10 < 9 > CFU / g. According to the invention, a combined prevention and control method taking antibacterial lipopeptide and bacillus subtilis as cores is adopted, so that the synergistic prevention and control of tobacco black shank and tomato spotted wilt are realized, and meanwhile, the agricultural and economic characters of tobacco are improved; due to the dual effects of the bacillus subtilis and the antibacterial lipopeptide, the lasting period can be prolonged, and the stability is high; the complex microbial inoculant can be completely biodegraded and is suitable for organic agriculture.

This invention discloses a pesticide composition containing silicon-rich rings and its application in controlling banana leaf spot disease, belonging to the field of biocide pesticide technology. The invention extracts pomegranate seeds. In indoor experiments, the colony diameter of *Curvularia natans* leaf spot pathogen was reduced, indicating that the pomegranate seed extract can inhibit the growth of *Curvularia natans*. Using this extract and silicon-rich rings as synergistic components, it is combined with propiconazole to prepare a pesticide composition containing silicon-rich rings. In field trials, the disease index of the plants decreased, indicating that *Curvularia natans* leaf spot pathogen was inhibited; the complex group showed the highest relative control efficacy, reaching 78.13%. The pesticide composition containing silicon-rich rings prepared by this invention can better inhibit *Curvularia natans* leaf spot pathogen and is suitable for pesticide development for controlling banana leaf spot disease.

The present application relates to the technical field of biology, and in particular discloses a PCR detection primer pair of Alternaria porri, a target and application thereof.The present application provides a PCR detection primer pair of Alternaria porri, and the nucleotide sequences of the primer pair are shown as SEQ ID No.1-2.After PCR amplification of DNA of a to-be-tested sample is carried out by using the primer pair of the present application, and then whether specific bands appear or not is observed by combining with gel electrophoresis, it can be determined whether Alternaria porri is contained in the to-be-tested sample or not.The primer has high specificity and good sensitivity, can rapidly detect the presence of Alternaria porri in a sample, and can provide a reference for early inspection and quarantine and early prevention and treatment of Alternaria porri leaf spot.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

The invention provides an OsWRKY19 gene and an application of the OsWRKY19 gene in resistance to cochliobolus miyabeanus of rice. A CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 (CRISPR associated protein 9) technology is adopted to obtain an OsWRKY19 knockout mutant (Oswrky19) and an overexpression strain of And after the cochliobolus miyabeanus pathogenic bacteria are inoculated, compared with a wild type, the knockout mutant is more susceptible to diseases, and the over-expression strain resistance is obviously enhanced. The OsWRKY19 overexpression obviously improves the callose deposition level after rice plants are induced by chitin, and further enhances the basic defensive reaction of rice. RT-qPCR discovers that the expression of the defensive reaction related gene OsMBL1 in a knockout mutant is reduced and is increased in an overexpression plant, which indicates that the OsWRKY19 can promote the expression of the OsMBL1, thereby enhancing the resistance of rice. In conclusion, the OsWRKY19 is a gene for positively regulating and controlling the resistance of the cochliobolus miyabeanus of the rice, and can promote the basic defense response of the rice and the expression of a disease-resistant related gene, so that the resistance of the rice to the cochliobolus miyabeanus is improved. The content of the invention provides important genetic resources and theoretical guidance for the breeding of the cochliobolus miyabeanus resistant variety of rice.

This invention discloses the application of *Polygonum aviculare* extract in the prevention and control of bacterial leaf spot in peach. The invention utilizes ultrasound-assisted extraction to extract the active components of *Polygonum aviculare*. Antibacterial experiments demonstrated that the extract exhibits good antibacterial activity against the pathogen of peach bacterial leaf spot (*Xanthomonas arboricola* pv. pruni, Xap), with a minimum inhibitory concentration (MIC) identical to that of the positive control drug oxytetracycline, both at 0.125 mg / mL. The antibacterial activity of *Polygonum aviculare* extract is comparable to that of oxytetracycline, making it a potential agent for the prevention and control of peach bacterial leaf spot.

The application discloses a rice OsNAR1 application of the gene or the coded protein in regulating rice resistance to Magnaporthe oryzae, and relates to the technical field of rice Magnaporthe oryzae prevention and treatment. OsNAR1 The CDS nucleotide sequence of the gene is shown as SEQ ID No. 2. OsNAR1 The application finds that the rice gene OsNAR1 plays an important role in the process of rice resistance to Magnaporthe oryzae, the number of leaf spots on the gene deletion mutant is less, the gene can be used for screening rice strains resistant to Magnaporthe oryzae, the expression amount of a basic resistance marker gene in the gene deletion mutant is significantly up-regulated, and it is indicated that OsNAR1 negatively regulates the expression of the basic resistance gene and can be used for preventing and treating Magnaporthe oryzae.

The application provides a cycloalkyl-substituted triazolopyridine amide compound and a preparation method and application thereof, and belongs to the technical field of pesticides. The cycloalkyl-substituted triazolopyridine amide compound provided by the application introduces an unsubstituted or substituted cyclohexyl group or an unsubstituted or substituted cyclopentyl group on a triazole ring, has excellent inhibitory effect on pepper Phytophthora capsici Leonian, and has an EC 50 as low as 0.3 micromole / L; and the cycloalkyl-substituted triazolopyridine amide compound and derivatives thereof also have broad-spectrum inhibitory activity on rice Helminthosporium leaf spot fungus, wheat Helminthosporium leaf spot fungus, brassica sclerotinia, wheat Gibberella zeae, wheat take-all fungus, tomato botrytis cinerea, potato late blight fungus, pepper Phytophthora capsici Leonian, tomato early blight fungus, rice bacterial wilt fungus, potato dry rot fungus, cucumber anthracnose fungus and rice rice blast fungus, and have a good application prospect in the preparation of anti-plant pathogenic fungus drugs.

The application discloses a pesticide composition containing silicon-rich rings and application thereof in prevention and treatment of cucumber bacterial angular leaf spot, and belongs to the technical field of biocidal pesticides. Grape seed extract and dracocephalum moldavica extract are obtained by extraction, in indoor tests, the colony diameters of Pseudomonas syringae pv. Lachrymans are reduced, which indicates that the grape seed extract and the dracocephalum moldavica extract can inhibit the growth of Pseudomonas syringae pv. Lachrymans. The two kinds of extracts are used as synergistic extracts, combined with silicon-rich rings and copper oxyquinoline, to prepare a pesticide composition containing silicon-rich rings. In field tests, the disease index of plants is reduced, which indicates that Pseudomonas syringae pv. Lachrymans is inhibited, and the relative control effect of the compound group is the highest, reaching 80.29%. The pesticide composition containing silicon-rich rings prepared by the application can better inhibit Pseudomonas syringae pv. Lachrymans, and is suitable for development of a pesticide for prevention and treatment of cucumber bacterial angular leaf spot.

The application belongs to the technical field of biocontrol preparation, and particularly relates to a traditional Chinese medicine composition, a biocontrol preparation and application in the field of pearl plant leaf spot nematode disease prevention and treatment. The leaf spot nematode disease is a main disease and pest of pearl plant planting, and there is no effective prevention and treatment method at present. The application provides a traditional Chinese medicine composition, which comprises wolfberry, leigongteng, peppermint, phellodendron, boerhaavia diffusa and chamomile. Water extracts of the above-mentioned medicinal ingredients can effectively reduce the number of nematodes in soil and improve the disease resistance of pearl plants when used for pearl plant irrigation. The research results of the application are helpful for restraining the occurrence of pearl plant leaf spot nematode disease through biological prevention and control means, and provide theoretical guidance for the development of pearl plant green industry.

This invention relates to the field of bio-breeding technology and discloses a molecular marker-assisted breeding method for bitter gourd resistant to powdery mildew and high-altitude leaf spot disease. The method includes constructing powdery mildew-resistant target-response probes and high-altitude leaf spot-resistant target-response probes; preparing mesoporous silica nanoparticles with a surface covalently coupled to a fluorescent internal reference molecule and a plant cell-penetrating peptide; loading the powdery mildew-resistant and high-altitude leaf spot-resistant target-response probes into the mesoporous channels to obtain a self-calibrated in-situ living nanoprobe complex; infiltrating the self-calibrated in-situ living nanoprobe complex into the living tissue of a single bitter gourd plant; collecting the total fluorescence intensity and spontaneous background fluorescence intensity and calculating the standardized resistance index; comparing the standardized resistance index with a judgment threshold to screen out dual-resistant living plants. This invention eliminates the physical error caused by fluctuations in the absolute amount of vector infiltration, achieving in-situ, non-destructive, and accurate identification of dual-resistant genotypes in living plants.

Plants, cells, tissues, and germplasms comprising genes and labeled alleles associated with increased resistance to gray leaf spot are provided. Also provided are methods of breeding plants having alleles associated with increased resistance to gray leaf spot and methods of identifying and selecting plants having alleles associated with increased resistance to gray leaf spot. Methods for identifying novel genes encoding proteins that provide resistance to gray leaf spot in plants and uses thereof are provided. The genes and marker alleles of the present disclosure can be used to produce gray leaf spot resistant plants by breeding, transgenic modification or genome editing.

This application relates to the field of pesticide fungicides, specifically disclosing a fungicide containing amino oligosaccharides and its preparation method. The fungicide containing amino oligosaccharides comprises the following raw materials in the following mass percentages: active ingredient 1.35-1.6%, synergistic agent 1.2-1.5%, wetting and dispersing agent 3-6%, antifreeze agent 5-8%, preservative 0.1-0.2%, and deionized water to 100%; the active ingredient comprises amino oligosaccharides and octochlor acetate in a mass ratio of 1:2-5. This application, by preparing a fungicide containing amino oligosaccharides, improves the antibacterial effect, avoids the development of drug resistance, and achieves efficient control of bacterial angular leaf spot disease in cucumbers.

The invention relates to the technical field of culture devices, and discloses a bipolaris maydis culture and observation device which comprises a culture box, a box door arranged on the culture box, an observation window arranged on the box door and a display panel arranged on the side wall of the culture box. A groove is formed in the middle of the placement frame, and a mounting seat is arranged in the culture cavity; through cooperative arrangement of the driving system, the mounting base and the macro camera in the culture cavity, the function of observing the growth condition of the bipolaris maydis in the culture dish without opening the box door is achieved, and the driving system can drive the mounting base to accurately move in the X direction, the Y direction and the Z direction; the microspur camera can move to the position over any target culture dish, shot images are displayed in real time through the display panel, and the problem that in a traditional observation mode, due to the fact that a box door is opened, external unfiltered and disinfected air enters a culture cavity is effectively solved.

The invention provides a composite antibacterial agent for garden plants and a preparation method thereof, and belongs to the technical field of plant disease control. The composite antibacterial agent comprises trichoderma, a synergistic auxiliary agent, a dispersing agent, a protective agent and a wetting agent, wherein the synergistic auxiliary agent is prepared by mixing sophocarpine, baicalin and arundinine in a mass ratio of (1-3): (2-7): (1-2). The composite antibacterial agent provided by the invention can effectively prevent and treat powdery mildew and leaf spot disease, and the prevention and treatment effects are both greater than 85%.

The invention relates to a biocontrol bacterium for preventing and treating alfalfa leaf spot disease, a biocontrol microbial agent and a preparation method and application thereof, and belongs to the field of microorganisms, the biocontrol bacterium MX282 belongs to Pantoea agglomerans, is preserved in China General Microbiological Culture Collection Center on June 23, 2025, has the preservation number of CGMCC No.34969, and has the preservation number of CGMCC No.34969. The biocontrol bacterium MX282 has the preservation number of CGMCC No.34969. The biocontrol bacterium MX282 has the preservation number of CGMCC No.34969 and the preservation number of CGMCC No.34969. Meanwhile, the invention discloses application of the biocontrol bacterium MX282 to prevention and treatment of alfalfa leaf spot disease and promotion of alfalfa growth.

The invention relates to a rice disease detection method based on a YOLO algorithm, in particular to high-precision detection achieved by improving a YOLOv11 model. The method combines a plurality of innovative modules, and aims to improve the detection accuracy of rice diseases (such as bacterial leaf spot disease, brown spot disease and leaf tumor disease). By introducing a CPA-Enhancer image enhancement module, a bidirectional feature pyramid network (BiFPN), a CARAFE content perception feature recombination module and a CBAM double attention mechanism, the small target identification problem of rice leaf scab can be effectively solved, and the challenges of background interference and multi-scale feature fusion in a complex field environment are overcome. According to the method, through multi-stage optimization, the recognition precision and the detection speed of the model on rice diseases are remarkably improved, and compared with a traditional method, the method has obvious advantages in the aspects of small target detection and scab boundary detail recovery. Experimental results show that the model of the invention is superior to the existing YOLO series and other common deep learning models in multiple performance indexes, and is suitable for large-scale field real-time monitoring and intelligent agriculture application.

The invention discloses an application of an RsBLV8 virus in preparation of a biocontrol preparation of tobacco target leaf spot bacteria, and belongs to the technical field of prevention and control of tobacco target leaf spot bacteria. According to the invention, a novel Benyviridae virus RsBLV8 is extracted from tobacco target leaf spot bacteria GZ-6 carrying a single virus, and the RNA (Ribonucleic Acid) sequence of the RsBLV8 virus is as shown in SEQ ID NO. 3. The pathogenicity of an original strain GZ-6 and the pathogenicity of detoxified tobacco target leaf pathogen GZ-6 are compared by using an in-vitro leaf method, and the statistics of the sizes of disease spots shows that the disease spots caused by the strains infected by the RsBLV8 are obviously smaller than those caused by homologous virus-free strains, so that the pathogenicity of fungi can be reduced by the virus RsBLV8; the compound can be applied to preparation of a biocontrol preparation for inhibiting pathogenicity of tobacco target leaf spot bacteria.

The invention relates to the technical field of banana leaf spot disease control, and discloses an epoxiconazole and saisentong mixed composition and application to banana leaf spot disease treatment, the epoxiconazole and saisentong mixed composition comprises the following components by mass: 26%-34% of an active composition, 1%-3% of a wetting agent, 3%-7% of a dispersing composition, 0.1%-0.5% of a thickening agent, 0.3%-0.8% of magnesium aluminum silicate, and the balance of deionized water, and the active composition is composed of epoxiconazole and saisentong. Fungus ergosterol synthesis is inhibited through a triazole action mechanism of epoxiconazole, meanwhile, copper ions released by Saisentong are combined to destroy a bacterial cell membrane protein structure, double killing of banana cephalosporium and pathogenic variant bacteria is achieved, broad-spectrum prevention and control of fungi and bacteria are achieved through synergistic compounding of epoxiconazole and Saisentong, and the synergistic effect of epoxiconazole and Saisentong is achieved. Therefore, the limitation of a single agent is broken through, efficient broad-spectrum prevention and control on a mixed pathogen system are realized, and the development of drug resistance is obviously delayed.

The present application belongs to the field of plant disease control research, and relates to a cucumber multi-host coelomyces effector protein CcSP1, a coding gene thereof and application. The present application provides a new cucumber multi-host coelomyces effector protein CcSP1, a coding gene thereof and application. The nucleotide sequence of the coding gene of the multi-host coelomyces effector protein CcSP1 is shown as SEQ ID NO. 1. Transient expression of the CcSP1 gene in N. benthamiana can inhibit hypersensitive necrosis caused by N. benthamiana. A gene deletion mutant ΔCcSP1 is constructed by homologous recombination, and the deletion of the gene does not affect the vegetative growth, but significantly reduces the pathogenicity when invading the cucumber leaves. It is shown that CcSP1 is involved in the lesion expansion of the plant after the invasion of the multi-host coelomyces, and plays an important role in the pathogenic process of the multi-host coelomyces. The present application has important significance for analyzing the related molecular mechanism of cucumber coelomyces leaf spot and establishing the comprehensive control technology strategy of the plant cucumber coelomyces leaf spot.

The invention discloses an application of long-chain non-coding RNA (Ribonucleic Acid) Cslnc256 in regulating and controlling an anti-colletotrichum gloeosporioides posttranscriptional mechanism of a tea tree. According to the application of the long-chain non-coding RNA Cslnc256 in regulating and controlling the colletotrichum gloeosporioides resisting post-transcription mechanism of the tea tree, the long-chain non-coding RNA Cslnc256 can accurately act on Cslnc256-CsmiR395-CsSultr2 in the tea tree body; therefore, the defense mechanism of the plant is efficiently activated. When the expression of the Cslnc256 is inhibited, the competitive binding capacity of the Cslnc256 and the CsmiR395 is reduced, so that the concentration of the free CsmiR395 is increased, and further, the activity of the Cslnc256 on the target gene CsSultr2; 1 is an inhibitory effect. The regulation and control process can reduce the activity of sulfate transporter protein in the tea tree body, so that sulfate ions are accumulated in leaves, and the sulfate ions serving as important defense signal molecules can effectively improve the resistance of the tea tree to colletotrichum gloeosporioides and relieve scab expansion and tissue damage caused by pathogenic bacterium infection.