206 results about "Leaf spot" patented technology

The invention discloses paenibacillus polymyxa K17 for preventing and treating diseases, the preservation number of the paenibacillus polymyxa K17 is CGMCC (China General Microbiological Culture Collection Center) No.33366, and the paenibacillus polymyxa K17 is classified and named as Paenibacillus polymyxa K17. The strain has a good anti-antagonism effect on 16 different plant pathogenic fungi from 8 crops; particularly, the antibacterial effect on various leaf spot diseases of tea trees is superior to that of part of common biocontrol strains such as Pristeria aryabhattai 11, Bacillus tequilensis R2 and the like. The control effect on tea tree leaf spot and tea tree anthracnose is higher than 70%, the control effect on black spot of picked tomatoes is 68%, the control effect is equivalent to that of various chemical pesticides, and the method has extremely high application prospects and market value in field biological control and postharvest preservation of plant diseases. Meanwhile, the strain K17 has the plant growth-promoting characteristics of secreting multiple extracellular hydrolases, dissolving inorganic phosphorus and mineral potassium and biologically fixing nitrogen, and has the potential of preparing plant growth-promoting microbial agents or bacterial fertilizers.

The invention belongs to the field of corn disease prevention, relates to a novel small peptide, provides a small peptide NCRP246 and application thereof in improvement of corn southern leaf blight resistance, and provides feasibility for plant disease resistance by using non-classical peptides. A corn southern leaf blight resistance identification experiment proves that NCRP246 can significantly improve the resistance of corn to southern leaf blight, and further researches that the NCRP246 reduces oxidative damage of pathogenic bacteria infection of the southern leaf blight to the corn by reducing H2O2 accumulation so as to mediate the resistance of the corn to the southern leaf blight; the analysis from the molecular perspective proves that the NCRP246 can induce the expression of defense related genes.

The invention provides a control method for apple alternaria leaf spot, and belongs to the technical field of crop disease control. The prevention and treatment method comprises the following steps: spraying a foliar biological pesticide fertilizer to apple trees; the foliar biological medical fertilizer comprises a bacillus fermentation product and a trichoderma fermentation product, the bacillus is at least one of bacillus amyloliquefaciens TS-1203, bacillus subtilis B1 and bacillus subtilis BS-1208, and the bacillus is at least one of bacillus amyloliquefaciens TS-1203, bacillus subtilis B1 and bacillus subtilis BS-1208; the trichoderma is at least one of T6 and Ym. The combined action of bacillus and trichoderma is utilized, the morbidity of apple alternaria leaf spot can be effectively reduced, the growth of apple trees can be promoted, the photosynthetic capacity is improved, the combination of disease prevention and yield increase is realized, and the method is environment-friendly and suitable for green agricultural development.

The invention relates to application of receptor-like kinase MdRLK2 in comprehensive resistance breeding of apples. After the receptor-like kinase MdRLK2 is instantaneously overexpressed in the apple tissue culture seedling Gal-3 and the apple Gala branch, it is found that the improvement of the expression quantity of the receptor-like kinase MdRLK2 effectively enhances the capability of resisting alternaria leaf spot, anthrax leaf blight, brown spot, rot disease and ring spot of apples; therefore, a molecular means for improving the comprehensive disease resistance of the apples is developed by overexpressing the apple receptor-like kinase MdRLK2, and meanwhile, a theoretical basis is provided for molecular breeding of the apples.

The invention provides a moniliella malayi and a fungicide and application thereof, and relates to the field of microorganisms. The acer rubrum is specifically acer rubrum RA, and the preservation number of the acer rubrum RA is CGMCC (China General Microbiological Culture Collection Center) No.35889. The acer rubrum RA can be used for preparing the acer rubrum. The strain has the capability of decomposing inorganic phosphorus, is wide in phosphorus-dissolving range, can decompose tricalcium phosphate, iron phosphate and aluminum phosphate, and particularly still has very strong capability of decomposing inorganic phosphorus under a low-temperature condition; the strain has the capability of producing siderophore and IAA at low temperature; the strain has an inhibition effect on various pathogenic bacteria causing field crop diseases, and can inhibit diseases such as corn root rot, sorghum leaf spot or wheat root rot; the strain can be colonized in a soil environment of 5-40 DEG C, a microbial inoculum prepared from the strain can promote crop growth, the crop yield is remarkably improved, and high yield can be achieved even if the usage amount of phosphate fertilizer is reduced.

The invention belongs to the technical field of fermentation engineering and microbial disease prevention and control, and particularly relates to application of microbacterium oxydans XZT347 in prevention and control of plant diseases. Experimental results show that the infection rates of the Microbacterium oxydans XZT347 to pepper root rot bacteria, cucumber target leaf spot bacteria, eggplant fusarium wilt bacteria, tomato gray mold bacteria and tea anthracnose bacteria are 10.0%, 13.3%, 20.0%, 6.7% and 10.0% respectively, and the morbidity of pepper root rot, cucumber target leaf spot, eggplant fusarium wilt, tomato gray mold and tea anthracnose is remarkably reduced.

The invention discloses bacillus subtilis S1703 and application thereof, and belongs to the technical field of plant disease and pest control. The name of the bacterial strain is bacillus subtilis S1703, and the preservation number of the bacterial strain is GDMCC No: 66890. The bacterial strain S1703 has a wider antibacterial spectrum, and can effectively inhibit the growth of pathogenic bacteria such as fusarium oxysporum, colletotrichum gloeosporioides, alternaria alternata, aspergillus, phyllosticta, citrus reticulata, fusarium and the like; fermentation liquor of the S1703 has a good antibacterial effect on the psidium guajava wilt pathogen and the psidium guajava branch blight pathogen; the bacteriostasis rate on sigatoka sigatoka and guava leaf spot germs reaches 95% or above, and an excellent bacteriostasis effect is achieved. The strain S1703 can avoid potential adverse effects of chemical pesticides on the environment and crop safety, and has potential commercial development and application values in biological prevention and control practice of diseases.

The invention provides a method for improving the disease resistance of plants, and particularly provides a method for improving the disease resistance of the plants by reducing or inhibiting the expression quantity and / or activity of SWEET11a genes or encoded proteins thereof. According to the method, the disease resistance of the plants can be improved, particularly the resistance to gray speck disease, northern leaf blight, bacterial wilt and stem rot is improved, and the method has important application value in biological breeding.

The invention discloses bacillus amyloliquefaciens BA-6 and application of the bacillus amyloliquefaciens BA-6 in prevention and treatment of corn leaf spot disease, and relates to the technical field of screening of biocontrol strains for prevention and treatment of corn leaf spot disease. The strain BA-6 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 27, 2024, the preservation number is CGMCC No.30157, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the strain is classified and named as Bacillus amyloliquefaciens. The strain can effectively antagonize Epicoccum nigrum, Epicoccum broomcorn, Helminthosporium maydis, Alternaria alternata and Helminthosporium bombycis, pot experiments show that fermentation broth inoculated with the strain can improve the prevention and treatment effect on the corn leaf spot disease, and a theoretical basis is provided for subsequent development of a biocontrol microbial inoculum for the corn leaf spot disease.

The invention discloses a novel leuconostoc screening method and an antibacterial mechanism of leuconostoc on leaf mold of zanthoxylum bungeanum, and relates to the technical field of pathogenic bacteria experiment and analysis. The novel leuconostoc screening method comprises the following steps that S1, materials are prepared, specifically, rotten Chinese prickly ash tree plants are selected, and tested strains in the Chinese prickly ash tree plants comprise plant pathogens of alternaria alternata, fusarium solani, fusarium layered, fusarium oxysporum, fusarium syphilis and colletotrichum pini, and are stored in a refrigerator of a laboratory at the temperature of 4 DEG C for standby application; in the invention, the leuconostoc also has an efficient inhibition effect on field crop pathogenic fungus alternaria alternata, and the research expands the biocontrol spectrum of lactic acid bacteria. Meanwhile, the invention discloses a molecular mechanism that the Leuconostoc suionicum HJ-L synergistically regulates and controls the synthesis of antibacterial substances through lipid metabolism, an ABC transport system and an autophagy pathway, and a theoretical basis and a practical basis are provided for the application of the strain in green prevention and control of the leaf mold of Chinese prickly ash.

The present invention discloses the application of coumarin-3-carboxylic acid in preventing and treating plant bacterial diseases. The coumarin-3-carboxylic acid provided by the present invention has a significant inhibitory effect on plant bacterial diseases. The plant bacterial diseases are oryzae bacterial leaf blight, oryzae bacterial leaf streak, citrus canker, cantaloupe bacterial fruit spot, tomato bacterial wilt, strawberry bacterial angular leaf spot, cabbage black rot, cabbage soft rot, potato soft rot, mango bacterial angular leaf spot, cassava bacterial wilt, cucumber bacterial angular leaf spot, kiwifruit canker and banana bacterial soft rot. The coumarin-3-carboxylic acid provided by the present invention has protective and therapeutic effects on melon bacterial fruit spot.

The invention provides a polygonatum kingianum root endophytic fungus Princasa crispa DHJ068 and application thereof, and relates to the technical field of crop disease control. The polygonatum kingianum root endophytic fungus Princephala crispa DHJ068 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2025748, and a fermentation liquor crude extract of the Princephala crispa DHJ068 can be applied to prevention and treatment of crop diseases. The invention overcomes the defects in the prior art, and the fermentation liquor crude extract of the polygonatum kingianum root endophytic fungus Prringtonia crispa DHJ068 can effectively inhibit the growth of Chinese cabbage soft rot bacteria and cucumber bacterial angular leaf spot bacteria, thereby achieving the purpose of certain biological control.

The present invention relates to the field of image processing technology, and in particular to a method for identifying and predicting lesions on crop leaves. The method obtains an RGB image of any monitoring image, records it as a target image, and obtains a leaf surface area in the target image; judges whether there is a suspected lesion area in the leaf surface area based on the difference in three-channel values ​​of each pixel point in the leaf surface area; if there is a suspected lesion area in the leaf surface area of ​​the target image, obtains the suspected lesion area in a historical target image that belongs to the same shooting position as the target image, and judges whether there is a real lesion area in the target image based on the change characteristics of the suspected lesion area in each historical target image and the suspected lesion area in the target image; if there is a real lesion area in the target image, obtains the severity of the lesion in the target image based on the difference between the target image and the real lesion area in each historical target image, predicts the spread rate of the lesion, and takes preventive and control measures in a timely manner.

This invention discloses a class of thiadiazole-benzohexacyclic compounds, their preparation methods, and applications. The structural formula is shown in Formula I. This invention introduces disulfide bond fragments into the thiadiazole-benzohexacyclic skeleton to obtain a novel class of thiadiazole-benzohexacyclic compounds. These compounds have a novel structure, a simple preparation method, readily available raw materials, and are unlikely to produce harmful byproducts. The compounds exhibit inhibitory effects against various plant pathogenic bacteria, particularly against *Citrus canker*, *Ralstonia solanacearum*, and *Bacterial angular leaf spot* of cucumber, demonstrating significant inhibitory effects. They can be used as agricultural bactericides and have great application value.

The present application belongs to the technical field of plant disease prevention and treatment, and particularly relates to a biocontrol bacterium for preventing and treating Guaiacum officinale anthracnose, a fermentation liquor, a biocontrol bacterium agent and application. Bacillus velezensis A biocontrol bacterium is isolated and screened from a microbial resource on a healthy Guaiacum officinale leaf, and is classified and named as Bacillus velezensis , which is preserved in the China General Microbiological Culture Collection Center on March 28, 2025, and has a preservation number of CGMCC No.34038; the biocontrol bacterium provided in the present application has good antagonistic effects on Colletotrichum gloeosporioides causing Guaiacum officinale anthracnose, on Stemphylium botryosum causing Guaiacum officinale leaf spot disease, on Botryosphaeria dothidea, on Alternaria alternata and on Pestalotiopsis versicolor capable of causing plant diseases. The biocontrol bacterium, the fermentation liquor and the biocontrol bacterium agent provided in the present application have potential application prospects in preventing and treating Guaiacum officinale anthracnose.

The application discloses a bacillus velezensis A13 wettable powder and application thereof, and belongs to the technical field of biological pesticides. The application aims to solve the problems of limited prevention and treatment effect of existing seed treatment, high labor intensity of foliar spraying and short effective period. The wettable powder comprises a mother powder prepared from fermentation liquor and diatomite, and a dispersant, a wetting agent, a stabilizer and a protective agent, wherein the total addition amount of the dispersant and the wetting agent is 5%-14% of the mass of the mother powder, the addition amount of the protective agent is 0.5%-2% of the mass of the mother powder, and the addition amount of the stabilizer is 1%-3% of the mass of the mother powder. The application can be used for preventing and treating root diseases caused by panax quinquefolius rust rot fungus and leaf diseases caused by panax quinquefolius alternaria leaf spot fungus, and promoting plant growth.

This invention relates to the field of agricultural pest control, and particularly to a compound nanogel sustained-release agent, its preparation method, and its application. This invention provides a compound nanogel sustained-release agent comprising bromonitol, a synergist, a carrier material, and a cross-linking agent, wherein the synergist includes one or more of kasugamycin, amino oligosaccharides, thiazolium zinc, and copper hydroxide. This invention prepares a nanogel sustained-release agent by compounding bromonitol and the synergist, which has significant preventive effects against plant bacterial diseases such as bacterial wilt, bacterial angular leaf spot, canker, and soft rot. Furthermore, the compound nanogel sustained-release agent of this invention has small particle size, stable physicochemical properties under cold and heat, good dispersibility in water, no particle agglomeration, no particle size increase, a decomposition rate of less than 5%, a long-lasting effect, good safety, is not prone to inducing drug resistance, requires a small dosage, and simultaneously increases yield and efficiency.

This invention relates to a method for detecting peanut leaf spot disease images in the canopy, overcoming the shortcomings of existing technologies in detecting peanut leaf spot disease in complex backgrounds. The invention includes the following steps: constructing a dataset; constructing a leaf spot disease detection model; training the leaf spot disease detection model; collecting and preprocessing high-resolution images of the peanut canopy to be detected; and obtaining the leaf spot disease detection results. This invention can handle the detection of small-target peanut leaf spot in complex backgrounds, balancing detection accuracy and real-time performance in the small-target detection problem, achieving real-time field-scale detection of peanut leaf spot disease in the canopy.

This invention relates to the field of microbial technology, specifically to a strain of *Bacillus laterosporus* and its applications. The *Bacillus laterosporus* provided by this invention (… Brevibacillus laterosporus HNBR05-1 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 25886. *Bacillus laterosporus* HNBR05-1 exhibits highly effective inhibition of plant pathogens, demonstrating dual efficacy against plant diseases such as brown spot and bacterial leaf spot, with high control efficacy. It shows promising application prospects in plant disease control and lays the foundation for the development of highly effective biocontrol agents.

The present application relates to a kind of oligonucleotide primer probe compositions for detecting Pseudomonas syringae, the pathogen of vegetable leaf spot disease based on ERA fluorescence method or ERA test strip method, and the detection method using the composition.The present application can simply, quickly, specifically, sensitively and visually broad-spectrum identify a variety of pathogenic variants of Pseudomonas syringae, the pathogen of vegetable leaf spot disease, the method is easy to operate, reaction time is short, without complex temperature control equipment, can be widely applied to the on-site rapid screening and disease diagnosis of Pseudomonas syringae in vegetable sample.

This invention belongs to the field of biological control technology for plant diseases and pests, and provides a strain of Chaetomium globosum ( Chaetomium globosum EF-14 and its applications. This strain is deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 40618. The *Chaetomium globosum* EF-14 provided by this invention is an endophytic fungus of maize that can simultaneously inhibit *Fusarium graminearum* (…). Fusarium gramineae ) and Corn spores ( Bipolaris maydis It can effectively reduce corn stalk rot and corn leaf spot caused by fall armyworm ( ) and can also effectively reduce the incidence of fall armyworm ( ). Spodoptera frugiperda This invention provides a highly efficient and safe microbial resource for the green control of major corn diseases and pests, and broadens the application scope of Chaetomium globosum in agricultural biocontrol, achieving synergistic control of diseases and pests through "one fungus targeting multiple targets".

The invention relates to a complex microbial inoculant and application thereof in prevention and control of tobacco black shank and tomato spotted wilt, and belongs to the technical field of prevention and control of tobacco diseases. The complex microbial inoculant comprises antibacterial lipopeptide and bacillus subtilis, wherein the mass ratio of the antibacterial lipopeptide to the bacillus subtilis is (0.5-1.2): 10; 0.08 wt% to 0.12 wt% of antibacterial lipopeptide powder is adopted as the antibacterial lipopeptide; the antibacterial lipopeptide comprises at least one of surfactin, iturin and abinogen; the viable count of the bacillus subtilis is greater than or equal to 2 * 10 < 9 > CFU / g. According to the invention, a combined prevention and control method taking antibacterial lipopeptide and bacillus subtilis as cores is adopted, so that the synergistic prevention and control of tobacco black shank and tomato spotted wilt are realized, and meanwhile, the agricultural and economic characters of tobacco are improved; due to the dual effects of the bacillus subtilis and the antibacterial lipopeptide, the lasting period can be prolonged, and the stability is high; the complex microbial inoculant can be completely biodegraded and is suitable for organic agriculture.

This invention discloses a pesticide composition containing silicon-rich rings and its application in controlling banana leaf spot disease, belonging to the field of biocide pesticide technology. The invention extracts pomegranate seeds. In indoor experiments, the colony diameter of *Curvularia natans* leaf spot pathogen was reduced, indicating that the pomegranate seed extract can inhibit the growth of *Curvularia natans*. Using this extract and silicon-rich rings as synergistic components, it is combined with propiconazole to prepare a pesticide composition containing silicon-rich rings. In field trials, the disease index of the plants decreased, indicating that *Curvularia natans* leaf spot pathogen was inhibited; the complex group showed the highest relative control efficacy, reaching 78.13%. The pesticide composition containing silicon-rich rings prepared by this invention can better inhibit *Curvularia natans* leaf spot pathogen and is suitable for pesticide development for controlling banana leaf spot disease.

The present application relates to the technical field of biology, and in particular discloses a PCR detection primer pair of Alternaria porri, a target and application thereof.The present application provides a PCR detection primer pair of Alternaria porri, and the nucleotide sequences of the primer pair are shown as SEQ ID No.1-2.After PCR amplification of DNA of a to-be-tested sample is carried out by using the primer pair of the present application, and then whether specific bands appear or not is observed by combining with gel electrophoresis, it can be determined whether Alternaria porri is contained in the to-be-tested sample or not.The primer has high specificity and good sensitivity, can rapidly detect the presence of Alternaria porri in a sample, and can provide a reference for early inspection and quarantine and early prevention and treatment of Alternaria porri leaf spot.

The invention provides an InDel marker for detecting tomato gray leaf spot disease resistance. The InDel marker is closely linked with a gene of a sixth chromosome SL4.0 ch06: 33855052-33933438 of a tomato. The InDel marker developed by the invention can realize accurate, rapid and high-throughput detection of the tomato gray leaf spot disease resistant sm6.1 gene, and can be applied to commercial breeding of tomatoes and genetic improvement application research of gray leaf spot disease resistance; important clues can be provided for research on group evolution, gene chips and the like.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

The invention relates to the technical field of plant disease recognition, and discloses an oriental cherry leaf spot disease recognition method and system based on a deep convolutional network, and the method comprises the steps: building a dual dynamic system mathematical model of plant growth and model evolution, constructing a feature extractor, a progressive knowledge base, a dual-memory system, a concept drift detector and a meta-learning optimizer for sensing the growth stage, and integrating a biotechnology co-adaptation framework to realize long-term cooperation between the oriental cherry leaf spot identification system and the oriental cherry growth cycle; according to the method, long-term cooperation of the identification system and the oriental cherry growth cycle is realized, the problem of contradiction between stability and adaptability of a traditional deep learning method in long-term plant monitoring is solved, the oriental cherry leaf spot identification system can be kept efficient and stable in the application process for many years, and frequent manual intervention and model updating are not needed.

The invention provides an OsWRKY19 gene and an application of the OsWRKY19 gene in resistance to cochliobolus miyabeanus of rice. A CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 (CRISPR associated protein 9) technology is adopted to obtain an OsWRKY19 knockout mutant (Oswrky19) and an overexpression strain of And after the cochliobolus miyabeanus pathogenic bacteria are inoculated, compared with a wild type, the knockout mutant is more susceptible to diseases, and the over-expression strain resistance is obviously enhanced. The OsWRKY19 overexpression obviously improves the callose deposition level after rice plants are induced by chitin, and further enhances the basic defensive reaction of rice. RT-qPCR discovers that the expression of the defensive reaction related gene OsMBL1 in a knockout mutant is reduced and is increased in an overexpression plant, which indicates that the OsWRKY19 can promote the expression of the OsMBL1, thereby enhancing the resistance of rice. In conclusion, the OsWRKY19 is a gene for positively regulating and controlling the resistance of the cochliobolus miyabeanus of the rice, and can promote the basic defense response of the rice and the expression of a disease-resistant related gene, so that the resistance of the rice to the cochliobolus miyabeanus is improved. The content of the invention provides important genetic resources and theoretical guidance for the breeding of the cochliobolus miyabeanus resistant variety of rice.

The invention relates to application of a small peptide SsSCP in improvement of plant disease resistance. The small peptide SsSCP is prepared by the following steps: connecting a gene of the small peptide SsSCP to an expression vector to prepare an SsSCP recombinant expression plasmid; transferring the SsSCP recombinant expression plasmid into an expression host to obtain an expression strain; performing induced culture on the expression strain to obtain an induced expression culture; and purifying the small peptide SsSCP from the induced expression culture. The expression and purification method of the small peptide SsSCP is optimized, the small peptide GST-SsSCP is synthesized and purified through microorganisms, and meanwhile the activity of the SsSCP is kept. By applying the GST-SsSCP protein expressed and purified in vitro, ROS outbreak of different plants can be triggered, and the GST-SsSCP protein has broad-spectrum MAMP activity. And the disease resistance of plants is improved. As a green and environment-friendly biological prevention and control means, the protein has a remarkable effect on enhancing the resistance of tomatoes and soybeans to sclerotinia sclerotiorum and the resistance of tomatoes to bacterial leaf spot.

The invention discloses Erwinia americana and application of the Erwinia americana in prevention and treatment of plant pathogenic fungi, and belongs to the technical field of agriculture. The Ewingella americana disclosed by the invention is Ewingella americana NJC80 and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M2025462, and the preservation date is March 13, 2025. The Ewingella americana (Ewingella americana) NJC80 has an inhibition effect on plant pathogenic fungus alternaria alternata (Alternania alternata), and can be used for preparing a preparation for preventing the peanut leaf spot disease.