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271 results about "Leaf spot" patented technology

Leaf spots are round blemishes found on the leaves of many species of plants, mostly caused by parasitic fungi or bacteria. A typical spot is "zonal", meaning it has a definite edge and often has a darker border. When lots of spots are present, they can grow together and become a blight or a blotch. Fungal spots are usually round or free-form in shape.

Broad-spectrum biocontrol bacterial strain barren water bacillus ZG18 and application of broad-spectrum biocontrol bacterial strain barren water bacillus ZG18

The invention discloses a broad-spectrum biocontrol strain water bacillus barren ZG18 and application thereof, and belongs to the technical field of plant disease control, and the broad-spectrum biocontrol strain water bacillus barren ZG18 is characterized in that the water bacillus barren ZG18 is water bacillus barren and is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the water bacillus barren ZG18 is CGMCC (China General Microbiological Culture Collection Center). The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China; the preservation date is September 6, 2024; the preservation number is CGMCC (China General Microbiological Culture Collection Center) The biocontrol bacterium strain disclosed by the invention has a relatively strong inhibition effect on pathogenic fusarium sporotrichum, fusarium graminearum, botrytis cinerea, rhizoctonia solani and corticosis cucurbitae, the prevention and treatment effect of the strain ZG18 on tobacco target leaf spot reaches up to 51.34%, the prevention and treatment effect of the strain ZG18 on tobacco damping-off in a pot experiment is 65.91%, and the strain ZG18 has a promoting effect on tobacco growth.
Owner:TOBACCO RESEARCH INSTITUTE OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES (QINGZHOU TOBACCO RESEARCH INSTITUTE OF CHINA NATIONAL TOBACCO COMPANY)

Jujube tree disease and insect pest identification method and system combined with visual technology

The invention relates to the technical field of computer vision, in particular to a jujube tree disease and insect pest recognition method and system combined with a vision technology, and the method comprises the steps: extracting closed edge contours of a disease leaf gray image, and obtaining a contour skeleton of each closed edge contour; based on the graphic features of each contour skeleton, obtaining a to-be-recognized region, analyzing the distribution of gradient amplitudes of the skeleton contour edge and inner pixel points and the gradient amplitude distribution of the skeleton contour outer pixel points, and obtaining an edge transition value of each to-be-recognized region; based on the overall distribution characteristics of the gradient amplitudes of all edge pixel points of the skeleton contour of each to-be-identified area, obtaining an edge energy value of each to-be-identified area; and determining an edge distinguishing value of each to-be-identified area, and obtaining a disease identification result of each to-be-identified area. The invention aims to improve the identification capability of the jujube brown spot and the jujube gray leaf spot and improve the identification precision of disease detection.
Owner:SHAANXI INST OF BIOLOGICAL AGRI +1

Sequence combination for detecting cochliobolus miyabeanus based on RPA-CRISPR / Cas12b and application

The invention belongs to the technical field of pathogenic microorganism detection, and particularly relates to a sequence combination for detecting cochliobolus miyabeanus based on RPA-CRISPR / Cas12b and application of the sequence combination. The CRISPR / Cas system contained in the composition comprises Cas12b and sgRNA, and the CRISPR / Cas system can be cooperatively used with a specific reaction system. The rapid detection method comprises the following steps: sample pretreatment: treating a sample to extract nucleic acid; performing amplification reaction: performing RPA amplification reaction on the extracted nucleic acid by using the composition, and applying a nucleic acid amplification product to CRISPR / Cas system detection; result interpretation: reading and judging a detection result through a quality control line and a detection line. The composition and the kit provided by the invention are specially used for detecting the cochliobolus miyabeanus. The method is simple and convenient to operate, rapid in detection, short in time consumption, low in cost and suitable for on-site rapid detection.
Owner:CHINA AGRI UNIV SANYA RES INST

Banana endophytic bacillus velezensis JDB15 and application thereof

The invention belongs to the field of microorganisms, and particularly relates to banana endophytic bacillus velezensis JDB15 and application thereof. The novel endophytic bacillus velezensis JDB15 is separated from banana plants, has the characteristics of efficient inhibition of Foc4, growth promotion of bananas and broad-spectrum antagonism of various pathogenic bacteria, can inhibit the morbidity of corn southern leaf blight, tomato fusarium wilt, tobacco gray mold and peach anthracnose, has the effects of inhibiting the morbidity of various pathogenic fungi, and has the advantages of high efficiency, low cost and the like. And a good broad-spectrum effect is achieved. Meanwhile, the strain can kill nematodes, has wide biocontrol bacterium activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other diseases and insect pests.
Owner:SANYA RES INST OF CHINESE ACAD OF TROPICAL AGRI +2

Application of turmeric rhizome extract missible oil in prevention and treatment of corn leaf spot disease and tomato gray mold

The invention relates to application of turmeric rhizome extract emulsifiable concentrate in preventing and treating corn leaf spot and tomato gray mold. Compared with the prior art, the turmeric rhizome extract missible oil disclosed by the invention contains an effective substance aromatic turmerone, and the aromatic turmerone can reduce the spore germination rate and sporulation quantity of pathogenic bacteria, increase the permeability of hypha cell membranes and cause overflow of contents, so that normal growth of hyphae is inhibited, and an antibacterial effect is achieved. Aromatic turmerone acts on plants, so that plant cells generate more polyphenol and flavonoid substances, and the immunity of the plants is improved. Pharmacodynamic test results in fields in two fields show that the 6.5% turmeric rhizome extract emulsifiable concentrate can be used for stably and effectively preventing and treating corn northern leaf blight and tomato gray mold after being diluted by 150-300 times, and also has a yield increasing effect.
Owner:SHANGHAI JIAOTONG UNIV

Biocontrol complex microbial inoculant for preventing and treating curvularia maize leaf spot disease and application of biocontrol complex microbial inoculant

The invention discloses a biocontrol complex microbial inoculant for preventing and treating maize curvularia leaf spot and application thereof, and belongs to the field of crop disease prevention and treatment. The biocontrol complex microbial inoculant comprises the following active ingredients: bacillus cereus, pseudomonas chlororaphis and streptomyces microflavus, and / or metabolites thereof. The beneficial microorganisms with different action mechanisms are combined, and the prevention and control effect on the curvularia maize leaf spot disease is enhanced through the synergistic effect. Natural microbial resources are adopted to replace traditional chemical pesticides, harmful substance residues are reduced, and the ecological environment is protected. Through reasonable matching of different types of beneficial microorganisms, effective prevention and control of the curvularia maize leaf spot disease are realized, and healthy growth of plants can be promoted. Compared with a single strain preparation, the complex microbial inoculant provided by the invention has stronger stability and durability, and can significantly reduce the incidence rate of diseases.
Owner:LIAONING ACAD OF AGRI SCI

Paenibacillus polymyxa K17 for disease control and application of paenibacillus polymyxa K17

The invention discloses paenibacillus polymyxa K17 for preventing and treating diseases, the preservation number of the paenibacillus polymyxa K17 is CGMCC (China General Microbiological Culture Collection Center) No.33366, and the paenibacillus polymyxa K17 is classified and named as Paenibacillus polymyxa K17. The strain has a good anti-antagonism effect on 16 different plant pathogenic fungi from 8 crops; particularly, the antibacterial effect on various leaf spot diseases of tea trees is superior to that of part of common biocontrol strains such as Pristeria aryabhattai 11, Bacillus tequilensis R2 and the like. The control effect on tea tree leaf spot and tea tree anthracnose is higher than 70%, the control effect on black spot of picked tomatoes is 68%, the control effect is equivalent to that of various chemical pesticides, and the method has extremely high application prospects and market value in field biological control and postharvest preservation of plant diseases. Meanwhile, the strain K17 has the plant growth-promoting characteristics of secreting multiple extracellular hydrolases, dissolving inorganic phosphorus and mineral potassium and biologically fixing nitrogen, and has the potential of preparing plant growth-promoting microbial agents or bacterial fertilizers.
Owner:GUIZHOU UNIV

Small peptide NCRP246 and application thereof in improving resistance to corn southern leaf blight

The invention belongs to the field of corn disease prevention, relates to a novel small peptide, provides a small peptide NCRP246 and application thereof in improvement of corn southern leaf blight resistance, and provides feasibility for plant disease resistance by using non-classical peptides. A corn southern leaf blight resistance identification experiment proves that NCRP246 can significantly improve the resistance of corn to southern leaf blight, and further researches that the NCRP246 reduces oxidative damage of pathogenic bacteria infection of the southern leaf blight to the corn by reducing H2O2 accumulation so as to mediate the resistance of the corn to the southern leaf blight; the analysis from the molecular perspective proves that the NCRP246 can induce the expression of defense related genes.
Owner:HENAN AGRICULTURAL UNIVERSITY

Prevention and treatment method for apple alternaria leaf spot

The invention provides a control method for apple alternaria leaf spot, and belongs to the technical field of crop disease control. The prevention and treatment method comprises the following steps: spraying a foliar biological pesticide fertilizer to apple trees; the foliar biological medical fertilizer comprises a bacillus fermentation product and a trichoderma fermentation product, the bacillus is at least one of bacillus amyloliquefaciens TS-1203, bacillus subtilis B1 and bacillus subtilis BS-1208, and the bacillus is at least one of bacillus amyloliquefaciens TS-1203, bacillus subtilis B1 and bacillus subtilis BS-1208; the trichoderma is at least one of T6 and Ym. The combined action of bacillus and trichoderma is utilized, the morbidity of apple alternaria leaf spot can be effectively reduced, the growth of apple trees can be promoted, the photosynthetic capacity is improved, the combination of disease prevention and yield increase is realized, and the method is environment-friendly and suitable for green agricultural development.
Owner:GANSU AGRI UNIV

Delftia palustris and application thereof in prevention and treatment of plant diseases

The invention discloses Delftia tsuhuensis and an application of the Delftia tsuhuensis in prevention and treatment of plant diseases. The Delftia tsuhuensis CDJ2 is preserved in the Guangdong Microbial Culture Collection Center on October 18, 2024, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 65290. The strain has a good prevention and treatment effect on citrus canker, and the prevention and treatment effect can reach 84%. Meanwhile, the strain can also effectively prevent and treat soft rot of taro, bacterial basal rot of rice, bacterial wilt of crops, kiwifruit canker, angular leaf spot of mango, black rot of brassicaceous vegetables, tomato scab, gummosis of sugarcane, bacterial wilt of walnuts and bacterial leaf spot of plums. Wide application prospects are realized in the aspects of preventing and treating diseases of crops and commercial crops and promoting healthy growth of plants.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Application of receptor-like kinase MdRLK2 in apple comprehensive resistance breeding

The invention relates to application of receptor-like kinase MdRLK2 in comprehensive resistance breeding of apples. After the receptor-like kinase MdRLK2 is instantaneously overexpressed in the apple tissue culture seedling Gal-3 and the apple Gala branch, it is found that the improvement of the expression quantity of the receptor-like kinase MdRLK2 effectively enhances the capability of resisting alternaria leaf spot, anthrax leaf blight, brown spot, rot disease and ring spot of apples; therefore, a molecular means for improving the comprehensive disease resistance of the apples is developed by overexpressing the apple receptor-like kinase MdRLK2, and meanwhile, a theoretical basis is provided for molecular breeding of the apples.
Owner:CHINA AGRI UNIV

Rhodoella malayi as well as fungicide and application of Rhodoella malayi

The invention provides a moniliella malayi and a fungicide and application thereof, and relates to the field of microorganisms. The acer rubrum is specifically acer rubrum RA, and the preservation number of the acer rubrum RA is CGMCC (China General Microbiological Culture Collection Center) No.35889. The acer rubrum RA can be used for preparing the acer rubrum. The strain has the capability of decomposing inorganic phosphorus, is wide in phosphorus-dissolving range, can decompose tricalcium phosphate, iron phosphate and aluminum phosphate, and particularly still has very strong capability of decomposing inorganic phosphorus under a low-temperature condition; the strain has the capability of producing siderophore and IAA at low temperature; the strain has an inhibition effect on various pathogenic bacteria causing field crop diseases, and can inhibit diseases such as corn root rot, sorghum leaf spot or wheat root rot; the strain can be colonized in a soil environment of 5-40 DEG C, a microbial inoculum prepared from the strain can promote crop growth, the crop yield is remarkably improved, and high yield can be achieved even if the usage amount of phosphate fertilizer is reduced.
Owner:SHAANXI FENGDAN BAILI BIOTECHNOLOGY CO LTD

Application of fungus fermentation liquor to prevention and treatment of areca leaf spot disease

The invention discloses application of fungus fermentation liquor to prevention and treatment of areca leaf spot disease. The invention relates to the technical field of agricultural biology, and provides application of fungus fermentation liquor in prevention and treatment of areca leaf spot disease. The fungus fermentation liquor is filtrate obtained by performing fermentation culture on specific fungi and filtering a culture solution to remove thalli; the specific fungus is phomopsis of RdRp protein from the PaFV1 virus through overexpression. The fungus fermentation liquor provided by the invention is sprayed on areca leaves, so that the occurrence of areca leaf spot disease can be obviously reduced, and good disease control potential is shown. The invention has important significance for preventing and treating areca leaf spot disease.
Owner:HAINAN UNIVERSITY SANYA NANFAN RESEARCH INSTITUTE +1

Application of burkholderia gladioli XC-1 in prevention and treatment of plant diseases

The invention discloses an application of Burkholderia gladioli XC-1 in prevention and treatment of plant diseases, the 16S rRNA sequence of the Burkholderia gladioli XC-1 is as shown in SEQ ID NO: 3, and the plant diseases are litsea cubeba shoot blight, okra root rot, durian leaf spot disease, cubilose fruit canker, agilawood root rot or cassava leaf spot disease. The invention finds that the Burkholderia gladioli XC-1 has better development value and application potential in the prevention and treatment of various plant diseases, and a new microbial strain resource is provided for the prevention and treatment of various plant diseases.
Owner:GUANGDONG OCEAN UNIVERSITY +2

Trichoderma Guizhou B10 strain and application thereof

The invention provides a trichoderma Guizhou B10 strain and application thereof, and relates to the technical field of agricultural microbial control. The trichoderma Guizhou B10 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No. 3.25634. The invention overcomes the defects in the prior art, and the strain can inhibit pathogenic fungi of malania oleifera leaf spot disease, such as pestalotiopsis, pestalotiopsis, clostridium neocarpum, botryosphaeria and physophaga fungi, can promote the growth of malania oleifera plants, and has both disease-resistant and growth-promoting functions on malania oleifera seedlings, such as malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, malania oleifera leaf spot disease resistance, and malania oleifera leaf spot disease resistance.
Owner:SOUTHWEST FORESTRY UNIVERSITY

Method for detecting corn leukoderma pathogenic bacteria based on RPA-LFD

The invention relates to the technical field of biology, and particularly discloses a method for detecting corn leukoderma pathogenic bacteria based on RPA-LFD (Recombinase Polymerase Amplification-Lateral Flow Dipstick), which comprises the following steps: a recombinase polymerase amplification RPA reaction system comprising an upstream primer PA-F2, a biotin-labeled downstream primer PA-R3-biotin and an nfo probe PA3-nfo-2; the lateral flow chromatography analysis LFD test strip is used for detecting an amplification product; a reaction buffer solution and a dilution buffer solution; a rapid and visual corn leaf spot detection method is developed by using a recombinase-mediated isothermal amplification (PRA) technology and combining an nfo probe with a lateral flow chromatography analysis and diagnosis method, and finally, an efficient and rapid corn leaf spot detection, prevention and control system is established by popularizing and applying a novel visual detection method in a field.
Owner:INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI

Application of microbacterium oxydans XZT347 in prevention and treatment of plant diseases

PendingCN121058690ABiocideBacteriaBiotechnologyMicrobacterium oxydans
The invention belongs to the technical field of fermentation engineering and microbial disease prevention and control, and particularly relates to application of microbacterium oxydans XZT347 in prevention and control of plant diseases. Experimental results show that the infection rates of the Microbacterium oxydans XZT347 to pepper root rot bacteria, cucumber target leaf spot bacteria, eggplant fusarium wilt bacteria, tomato gray mold bacteria and tea anthracnose bacteria are 10.0%, 13.3%, 20.0%, 6.7% and 10.0% respectively, and the morbidity of pepper root rot, cucumber target leaf spot, eggplant fusarium wilt, tomato gray mold and tea anthracnose is remarkably reduced.
Owner:HUNAN INST OF MICROBIOLOGY +1

Streptomyces griseoaurantiacus HMQAUfx21024, biocontrol inoculant and preparation method and application of biocontrol inoculant

The invention relates to the field of biocontrol of plant diseases. The invention provides a Streptomyces griseoaurantiacus strain HMQAUfx21024, the strain is preserved in the China General Microbiological Culture Collection Center on January 3, 2025, and the preservation registration number of the strain is CGMCC No. 33290. The invention further provides a preparation method of the strain HMQAUfx21024, and the strain HMQAUfx21024 has the advantages that the strain HMQAUfx21024 can be used for preparing the strain HMQAUfx21024; the invention also provides a biocontrol inoculant. The invention also provides a preparation method of the biocontrol inoculant. The invention also provides application of the strain HMQAUfx21024 or the biocontrol microbial inoculum as described above or the biocontrol microbial inoculum prepared by the preparation method as described above. The invention further provides a method for preventing and treating plant diseases. The biocontrol agent is specially developed for cucumber downy mildew and target leaf spot, due to the fact that the biocontrol agent is a biological agent, a series of problems caused by use of chemical pesticides are completely avoided, pollution-free production of greenhouse vegetables is facilitated, farmers can not use or reduce the use amount of other chemical pesticides, expenses can be saved for the farmers, and the cost is reduced. Vegetable export is facilitated, and the income of farmers can be increased.
Owner:QINGDAO AGRI UNIV

Biocontrol bacterium for preventing and treating idesia polycarpa anthracnose, fermentation liquor, biocontrol bacterium agent and application

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to a biocontrol bacterium for preventing and treating idesia anthracnose, fermentation liquor, a biocontrol bacterium agent and application. According to the invention, a biocontrol bacterium is separated and screened from microbial resources on healthy idesia leaves, is classified and named as Bacillus velezensis, is preserved in China General Microbiological Culture Collection Center on March 28, 2025, and has a preservation number of CGMCC No.34038; the biocontrol bacterium provided by the invention has a good antagonistic effect on colletotrichum causing idesia anthracnose, melanospora causing idesia leaf spot, botrytis cinerea, fusarium oxysporum, alternaria alternata and pestalotiopsis which can cause plant diseases, and also has a good antagonistic effect on colletotrichum causing idesia anthracnose, melanospora causing idesia leaf spot, fusarium oxysporum, alternaria alternata and pestalotiopsis. The biocontrol bacterium, the fermentation broth and the biocontrol inoculant provided by the invention have potential application prospects in prevention and treatment of idesia anthracnose.
Owner:GUIZHOU EDUCATION UNIV

Bacillus subtilis S1703 and application thereof

The invention discloses bacillus subtilis S1703 and application thereof, and belongs to the technical field of plant disease and pest control. The name of the bacterial strain is bacillus subtilis S1703, and the preservation number of the bacterial strain is GDMCC No: 66890. The bacterial strain S1703 has a wider antibacterial spectrum, and can effectively inhibit the growth of pathogenic bacteria such as fusarium oxysporum, colletotrichum gloeosporioides, alternaria alternata, aspergillus, phyllosticta, citrus reticulata, fusarium and the like; fermentation liquor of the S1703 has a good antibacterial effect on the psidium guajava wilt pathogen and the psidium guajava branch blight pathogen; the bacteriostasis rate on sigatoka sigatoka and guava leaf spot germs reaches 95% or above, and an excellent bacteriostasis effect is achieved. The strain S1703 can avoid potential adverse effects of chemical pesticides on the environment and crop safety, and has potential commercial development and application values in biological prevention and control practice of diseases.
Owner:PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI

Bacillus, fungicide and application of bacillus in plant disease control and growth promotion

The invention discloses bacillus, a fungicide and application of the fungicide in plant disease control and growth promotion, and relates to the technical field of biocontrol bacteria. The preservation number of the bacillus is CGMCC (China General Microbiological Culture Collection Center) No.32658 The bacillus has a broad-spectrum antibacterial effect on various plant rhizosphere disease pathogenic fungi, can effectively prevent and control eucommia ulmoides leaf gray mold, leaf blight and leaf spot disease, can promote plant growth by secreting IAA (indoleacetic acid) and can provide necessary nutritional ingredients for plant growth by dissolving inorganic phosphorus, so that the bacillus has a broad-spectrum antibacterial effect on various plant rhizosphere disease pathogenic fungi and can be used for preventing and controlling eucommia ulmoides leaf gray mold, leaf blight and leaf spot disease. The bacillus provided by the invention has the functions of broad-spectrum fungal disease resistance and growth promotion.
Owner:NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS

Method for improving disease resistance of plants

The invention provides a method for improving the disease resistance of plants, and particularly provides a method for improving the disease resistance of the plants by reducing or inhibiting the expression quantity and / or activity of SWEET11a genes or encoded proteins thereof. According to the method, the disease resistance of the plants can be improved, particularly the resistance to gray speck disease, northern leaf blight, bacterial wilt and stem rot is improved, and the method has important application value in biological breeding.
Owner:SHANDONG SHUNFENG BIOTECH CO LTD

Bacillus deserticola for resisting tobacco target leaf spot, bacterial drug compound agent and application of bacillus deserticola and bacterial drug compound agent

The invention relates to the technical field of microorganisms, and particularly discloses bacillus deserticola for resisting tobacco target leaf spot, a bacterial drug compound and application of the bacillus deserticola and the bacterial drug compound. The bacillus deserticola is bacillus deserticola 127-2 and is preserved in the China General Microbiological Culture Collection Center on April 1, 2025, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing, the preservation number is CGMCC No.34058, and the bacillus deserticola is classified and named as Bacillus inaquorum; the in-vessel inhibition rate of the strain 127-2 to tobacco target leaf spot caused by rhizoctonia solani is 87.5%, the in-vivo inhibition activity also reaches 50% or above, the in-vivo inhibition activity of the strain 127-2 to tobacco target leaf spot caused by compounding of the strain 127-2 and pyraclostrobin reaches 98% or above, and the purposes of drug reduction, persistent effect and efficient prevention and control of rhizoctonia solani can be effectively achieved through the synergistic effect of bacteria and drugs.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY

Bacillus amyloliquefaciens BA-6 and application thereof in prevention and treatment of corn leaf spot disease

The invention discloses bacillus amyloliquefaciens BA-6 and application of the bacillus amyloliquefaciens BA-6 in prevention and treatment of corn leaf spot disease, and relates to the technical field of screening of biocontrol strains for prevention and treatment of corn leaf spot disease. The strain BA-6 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 27, 2024, the preservation number is CGMCC No.30157, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the strain is classified and named as Bacillus amyloliquefaciens. The strain can effectively antagonize Epicoccum nigrum, Epicoccum broomcorn, Helminthosporium maydis, Alternaria alternata and Helminthosporium bombycis, pot experiments show that fermentation broth inoculated with the strain can improve the prevention and treatment effect on the corn leaf spot disease, and a theoretical basis is provided for subsequent development of a biocontrol microbial inoculum for the corn leaf spot disease.
Owner:INST OF PLANT PROTECTION FAAS

Screening method of novel leuconostoc and bacteriostatic mechanism of leuconostoc on leaf mold of zanthoxylum bungeanum

The invention discloses a novel leuconostoc screening method and an antibacterial mechanism of leuconostoc on leaf mold of zanthoxylum bungeanum, and relates to the technical field of pathogenic bacteria experiment and analysis. The novel leuconostoc screening method comprises the following steps that S1, materials are prepared, specifically, rotten Chinese prickly ash tree plants are selected, and tested strains in the Chinese prickly ash tree plants comprise plant pathogens of alternaria alternata, fusarium solani, fusarium layered, fusarium oxysporum, fusarium syphilis and colletotrichum pini, and are stored in a refrigerator of a laboratory at the temperature of 4 DEG C for standby application; in the invention, the leuconostoc also has an efficient inhibition effect on field crop pathogenic fungus alternaria alternata, and the research expands the biocontrol spectrum of lactic acid bacteria. Meanwhile, the invention discloses a molecular mechanism that the Leuconostoc suionicum HJ-L synergistically regulates and controls the synthesis of antibacterial substances through lipid metabolism, an ABC transport system and an autophagy pathway, and a theoretical basis and a practical basis are provided for the application of the strain in green prevention and control of the leaf mold of Chinese prickly ash.
Owner:LONGNAN NORMAL COLLEGE

Loop-mediated isothermal amplification detection primer group for Rhodospora pini Maxidicola and application of loop-mediated isothermal amplification detection primer group for Rhodospora pini Maxidicola

The invention discloses a loop-mediated isothermal amplification detection primer group for Rhodospora pini Rhodospora acuminata and an application of the loop-mediated isothermal amplification detection primer group for Rhodospora pini Rhodospora acuminata. The invention designs four groups of loop-mediated isothermal amplification detection primer groups of Rhodospora pini Maxillaris, which are respectively a primer combination T1, a primer combination R1, a primer combination R3 or a primer combination R5, of Rhodospora pini Maxillaris. Experimental results show that the loop-mediated isothermal amplification detection primer group and the loop-mediated isothermal amplification detection method of the pine needle red spot pathogen botrytis aculeatus by using the loop-mediated isothermal amplification detection primer group have the characteristics of high specificity, low requirements on instruments and equipment, convenience in operation and the like; in production practice, rapid, sensitive, accurate, safe and visual detection of the pine needle red spot pathogen can be achieved, the method can be used for field investigation and early diagnosis of the pine needle red spot pathogen, quarantine and transportation of nursery stocks and disease monitoring and identification, and reliable technical and theoretical bases are provided for early warning and prevention and control of the pine needle red spot pathogen.
Owner:INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY

Bacillus velezensis, microbial inoculum and application of microbial inoculum

The invention discloses bacillus velezensis, a microbial inoculum and application of the bacillus velezensis in prevention and treatment of soybean gray speck disease. The bacillus velezensis YD21 has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.3334 in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. The bacillus velezensis YD21 provided by the invention is from seeds of wild soybeans LYD120068, can be used for effectively antagonizing 16 plant pathogenic fungi such as soybean gray leaf spot germs, corn black bundle germs, apple core germs, corn gray leaf spot germs and the like, and has an obvious prevention and treatment effect on the soybean gray leaf spot disease, so that the bacillus velezensis YD21 can be used for preventing and treating the soybean gray leaf spot disease, and the bacillus velezensis YD21 can be used for preventing and treating the soybean gray leaf spot disease. The method has a wide application prospect in the development of biological pesticides and biological organic fertilizers.
Owner:SHENYANG AGRI UNIV

Application of coumarin-3-carboxylic acid in preventing and controlling plant bacterial diseases

The present invention discloses the application of coumarin-3-carboxylic acid in preventing and treating plant bacterial diseases. The coumarin-3-carboxylic acid provided by the present invention has a significant inhibitory effect on plant bacterial diseases. The plant bacterial diseases are oryzae bacterial leaf blight, oryzae bacterial leaf streak, citrus canker, cantaloupe bacterial fruit spot, tomato bacterial wilt, strawberry bacterial angular leaf spot, cabbage black rot, cabbage soft rot, potato soft rot, mango bacterial angular leaf spot, cassava bacterial wilt, cucumber bacterial angular leaf spot, kiwifruit canker and banana bacterial soft rot. The coumarin-3-carboxylic acid provided by the present invention has protective and therapeutic effects on melon bacterial fruit spot.
Owner:ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI

Polygonatum kingianum root endophytic fungus Princasa crispa DHJ068 and application thereof

The invention provides a polygonatum kingianum root endophytic fungus Princasa crispa DHJ068 and application thereof, and relates to the technical field of crop disease control. The polygonatum kingianum root endophytic fungus Princephala crispa DHJ068 is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2025748, and a fermentation liquor crude extract of the Princephala crispa DHJ068 can be applied to prevention and treatment of crop diseases. The invention overcomes the defects in the prior art, and the fermentation liquor crude extract of the polygonatum kingianum root endophytic fungus Prringtonia crispa DHJ068 can effectively inhibit the growth of Chinese cabbage soft rot bacteria and cucumber bacterial angular leaf spot bacteria, thereby achieving the purpose of certain biological control.
Owner:YUXI NORMAL UNIV +1

A method for identifying and predicting crop leaf spots

The present invention relates to the field of image processing technology, and in particular to a method for identifying and predicting lesions on crop leaves. The method obtains an RGB image of any monitoring image, records it as a target image, and obtains a leaf surface area in the target image; judges whether there is a suspected lesion area in the leaf surface area based on the difference in three-channel values ​​of each pixel point in the leaf surface area; if there is a suspected lesion area in the leaf surface area of ​​the target image, obtains the suspected lesion area in a historical target image that belongs to the same shooting position as the target image, and judges whether there is a real lesion area in the target image based on the change characteristics of the suspected lesion area in each historical target image and the suspected lesion area in the target image; if there is a real lesion area in the target image, obtains the severity of the lesion in the target image based on the difference between the target image and the real lesion area in each historical target image, predicts the spread rate of the lesion, and takes preventive and control measures in a timely manner.
Owner:XIAN ZHUOCHEN NETWORK TECH CO LTD