The invention discloses seedling culturing substrates. The seedling culturing substrates are made from the following components in parts by weight: 21-33 parts of rice chaff, 55-85 parts of saw dust, 15-22 parts of coconut chaff, 1-5 parts of gypsum powder, 10-45 parts of bagasse, 55-75 parts of sheep manure, 9-13 parts of modified diatomite, 20-50 parts of kaolin, 25-35 parts of chicken manure, 5-10 parts of amidinothiourea and 8-15 parts of chloromethyl pyridine. The seedling culturing substrates disclosed by the invention are made from the rice chaff, the saw dust, the coconut chaff, the gypsum powder, the bagasse, the sheep manure, the modified diatomite, the kaolin, the chicken manure, the amidinothiourea and the chloromethyl pyridine in a specific compounding ratio, can perfectly replace turf to be used as soilless culturing substrates, and can well provide nutrients for lycopersicon esculentum in soilless culture.