116 results about "Root length" patented technology

The invention belongs to the technical field of agricultural production, and discloses an evaluation method for identifying the preliminary salt tolerance of foxtail millet in a germination period, which comprises the following steps: determining five key indexes of relative germination potential, relative germination rate, relative root length, relative bud length and relative germination index in the germination period of foxtail millet; the salt tolerance of the millet germplasm resources in the germination period can be preliminarily evaluated by establishing a regression equation to estimate the D value. The simple, convenient and scientific method for identifying the salt tolerance of the millet germplasm resources in the germination period is established, convenience is provided for screening salt-tolerant germplasm of millet, and the method is a premise for carrying out research on a salt-tolerant mechanism of millet.

The invention relates to the technical field of teasel breeding, and discloses a teasel germplasm resource multi-character collaborative screening breeding method, which comprises the following steps: multi-omics data acquisition: extracting teasel germplasm leaf DNA (deoxyribonucleic acid), and capturing exon regions of disease resistance and metabolism related genes by adopting a targeted sequencing technology to obtain an SNP (single nucleotide polymorphism) marker matrix; the method comprises the following steps: collecting root extracts, detecting medicinal components through ultra-high performance liquid chromatography-mass spectrometry, and constructing a metabolite abundance matrix; measuring phenotype data of plant height, root length, root weight, root rot morbidity and survival rate under drought stress; key site and metabolite correlation analysis: constructing a Bayesian network containing SNP markers, metabolite and phenotypes by using a graph database, and carrying out cross validation through a leave-one-out method. The dipsacus asper germplasm resource multi-character collaborative screening breeding method aims at solving the problem that a traditional breeding method lacks systematic integration analysis of genomes and metabolic pathways in dipsacus asper multi-character collaborative improvement.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention belongs to the field of muskmelons, and particularly relates to a muskmelon seedling stage root system premature ability evaluation method. According to the melon seedling stage root system early emergence capability evaluation method, black soil is used as a medium to cultivate plant seedlings in batches, image analysis and data acquisition are facilitated, the measured main root length, lateral root total length and lateral root number variation range distribution is uniform and continuous, variation difference is remarkable, and the method is suitable for large-scale popularization and application. The difference of root growth conditions of different plant materials can be reflected. The evaluation method provided by the invention can be used for evaluating the early germination capability of different muskmelon materials, so that the experiment time is saved, the experiment repeatability is high, the stability is high, the data reproducibility, repeatability and stability are relatively good, the evaluation of the early germination capability of the root systems of a plurality of muskmelon materials can be completed at one time, and the evaluation method has a wide application prospect. The high-throughput evaluation of the early-growing capability of the melon root system is realized.

The invention provides a salt-tolerant gene, namely a PrABC1 gene and application thereof, and also provides a vector containing the gene, an expression cassette, a recombinant cell, a transgenic plant and a method for producing the transgenic plant. Specifically, the gene has a nucleotide sequence as shown in SEQ ID NO: 1 or SEQ ID NO: 2. The plant growth regulator can be used for improving the tolerance of plants to a salt stress environment, relieving the adverse effects of a high-salt environment on the germination rate and root length of the plants, reducing the water loss rate of the plants under the high-salt stress condition and reducing the reduction of the chlorophyll content.

The invention relates to a growth-promoting compound microbial agent, which is prepared from bacillus pseudomycoides, bacillus subtilis and Trichoderma longibrachiatum, and is characterized in that the growth-promoting compound microbial agent is prepared from bacillus pseudomycoides, bacillus subtilis and Trichoderma longibrachiatum, and the growth-promoting compound microbial agent is prepared from bacillus amyloliquefaciens, bacillus subtilis and Trichoderma longibrachiatum. The compound microbial agent (bacillus subtilis, pseudofungal bacillus and trichoderma longibrachiatum) has excellent performance in the aspects of promoting plant growth and improving the soil environment, and the plant height, the fresh weight and the root length of plants are remarkably increased. The application of the complex microbial inoculant effectively relieves the chive continuous cropping obstacle, improves the chive growth potential and soil fertility, and provides a theoretical basis and technical support for sustainable planting of chives in facility agriculture.

The invention discloses a bacterial strain C5A-1 capable of producing lipopeptide and having a plant growth promoting function and application of the bacterial strain C5A-1, solves the technical problem of promoting plant growth, especially root length, plant height, stem diameter, leaf number, leaf area, underground dry weight and overground dry weight, and particularly discloses bacillus velezensis C5A-1 or a variant or a progeny thereof. The bacillus velezensis C5A-1 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32880. When the bacteria or the fermented supernate thereof or the combination thereof is applied to plants, the growth of the plants can be promoted, and the bacteria can be used for industrial production.

The invention discloses application of alfalfa VIK protein in cultivation of phytophthora root rot resistant plants, and belongs to the technical field of biology. The technical problem to be solved by the invention is how to improve the disease resistance of plants. The VIK protein disclosed by the invention is a protein of which the amino acid sequence is SEQ ID No. 1 or SEQ ID No. 4. Experiments prove that after the phytophthora phytopathogen is inoculated, compared with a wild plant, the mutant obtained by editing the VIK coding gene has the advantages that the disease resistance is enhanced, the survival rate is improved, the fresh weight, the overground part height, the root length and the plant height of the plant are increased, and the relative conductivity of leaves is reduced. Therefore, the resistance of the plant to the phytophthora phytopathogen is improved through the mutation of the VIK protein and the coding gene thereof, the coding gene of the VIK can be targeted through a gene editing technology, a VIK gene knockout plant is obtained, and a plant resisting the phytophthora phytopathogen is cultivated.

The invention relates to the technical field of microorganisms, in particular to a strain (Pezicula ericae) XA4 and application of the strain (Pezicula ericae) XA4. The invention provides a strain (Pezicula ericae) XA4 which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on June 26, 2024, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.41350. The invention also provides a method for preparing the strain (Pezicula ericae) XA4. The XA4 provided by the invention is used for carrying out tie-back on sterile cultured rhododendron dauricum seedlings, and trypan blue dyeing detection shows that mycorrhizal fungi can be successfully subjected to tie-back. Compared with the non-inoculated strain, the inoculated XA4 has the most obvious growth promoting effect on the rhododendron dauricum. The plant height, the leaf length, the leaf width, the root length, the biomass, the chlorophyll content, the net photosynthetic rate, the stomatal conductance, the intercellular CO2 concentration, the transpiration rate and the root activity of the strain are all improved compared with those of the strain without inoculation.

The invention provides a cultivation method for improving the yield and quality of pea tips by means of photoperiod and boron coordinated regulation and control. The cultivation method comprises the following steps that S1, pea seeds are disinfected and aeroponically cultivated; s2, pea sprouting seeds with the root length of 2-6 cm are selected and further cultured; meanwhile, in the culture process, a basic nutrient solution comprises the following components: 2.5 mM of Ca (NO3) 2.4 H2O, 1 mM of KH2PO4, 1 mM of KCl, 2 mM of MgSO4. 7H2O, 2.0 mM of K2SO4, 50 [mu] M of FeSO4. 7H2O, 50 [mu] M of EDTA.Na2, 2 [mu] M of MnCl2, 4 [mu] M of ZnSO4. 7H2O, 0.5 [mu] M of CuSO4. 5H2O, 1 [mu] M of (NH4) 6Mo7O24. 4H2O, 25-50 [mu] M of H3BO3 and 1 [mu] M of KI; nutrient elements are applied according to the following steps: (1) a first nutrient solution is adopted in 1-3 days, and the concentration of all components in the first nutrient solution is 10-15% of that of a basic nutrient solution; (2) after 4-8 days, adopting a second nutrient solution, wherein the concentration of all components in the second nutrient solution is 28-38% of that of the basic nutrient solution; and (3) adopting a third nutrient solution in 9-15 days, wherein the concentration of all components in the third nutrient solution is 90-100% of that of the basic nutrient solution. The cultivation method can effectively improve the yield and quality of the pea tips.

The application provides a method for preventing and controlling plant aphids, and the specific steps are as follows: selecting plant germination seeds that have grown roots and the root length is 2-6 cm, applying a light cycle treatment of 8h light and 16h darkness in the growth process of the plant germination seeds; in the plant germination seeds, the seed sources include legumes and cruciferous vegetables; and in the growth process of the plant germination seeds, a boron-containing nutrient solution containing the following components is used: Ca(NO3)2.4H2O 4mM, KH2PO4 1mM, KCl 1mM, MgSO4.7H2O 2mM, K2SO40.5mM, FeSO4.7H2O 50μM, EDTA.Na2 50μM, MnCl2 10μM, ZnSO4.7H2O 5μM, CuSO4.5H2O 0.5μM, (NH4)6Mo7O 24 4H2O 1μM, H3BO3 35-70μM, CoCl20.5μM; the nutrient elements are applied according to the following steps: (1) the first boron-containing nutrient solution is used for 1-5d, and the concentration of all components in the first boron-containing nutrient solution is 20-30% of that of the boron-containing nutrient solution; (2) the second boron-containing nutrient solution is used for 6-15d, and the concentration of all components in the second boron-containing nutrient solution is 40-60% of that of the boron-containing nutrient solution. The method can effectively prevent and control plant aphids.

The invention relates to the technical field of plant root system measurement, and discloses a plant seedling culture and root system observation hydroponic device which comprises a hydroponic tank with an opening in one side, an observation plate is mounted at the opening of the hydroponic tank, and guide mechanisms fixedly connected with the hydroponic tank are mounted at the top and the bottom of the observation plate. An adjusting and locking mechanism is slidably connected between the two groups of guide mechanisms, the adjusting and locking mechanism is connected with a measuring mechanism, and a covering plate which is of a U-shaped structure and slidably connected with the guide mechanisms is mounted on the side, away from the hydroponic tank, of the measuring mechanism. By means of the device, plants can be conveniently fixed and taken out, plant roots are not damaged during fixing and taking out, follow-up cultivation and measurement of the plants are facilitated, and the situation that measurement is inaccurate due to damage of the plant roots is reduced; the root system measurement requirements at different positions are met; influence of illumination on root growth is avoided; the requirements for measuring the root length and the angle deviating from the gravity vector are met, cultivation personnel can measure the root conveniently, and the measuring efficiency is improved.

The application relates to the field of microbial technology and discloses a Pseudomonas asiatica HNHMPs-260 and application thereof. The strain is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 66854. Fermentation supernatant of the strain can prevent and treat root-knot nematode disease of Hami melon caused by Meloidogyne javanica. Experiments show that the fermentation supernatant has strong toxic effects on second instar larvae of root-knot nematodes and can significantly inhibit the hatching of egg masses and egg grains. In addition, the fermentation supernatant has good thermal stability and storage stability, and still maintains more than 80% nematocidal activity after high-temperature treatment at 121 DEG C and long-term storage at 4 DEG C. Meanwhile, the fermentation supernatant of the strain can promote the growth of Hami melon plants, improve physiological indexes such as root length, stem length, fresh weight and dry weight, and has the application potential of being developed into efficient, environment-friendly and multifunctional biological pesticides.

This application discloses a method for systematically monitoring and evaluating the structural and functional diversity of urban forest stands. It identifies the plant type by analyzing the ratio of leaf to leaf root length among different plants, combined with pigment information of plants of the same type. The ratio of leaf to leaf root length is a prominent feature of plant leaves, and identifying this feature through binarized images is fast and accurate. However, identifying plant types solely based on the ratio of leaf to leaf root length or plant pigment information can lead to low accuracy because some plants have similar ratios, and different plants may have similar pigment information. This application combines these two features, using the ratio of leaf to leaf root length and plant pigment information to comprehensively determine the plant type, thus greatly improving the comprehensiveness of the features and increasing the accuracy of identification.

The invention discloses a plant growth-promoting bacterium and application thereof, relates to the field of microorganisms, and provides a plant growth-promoting bacterium which can generate a growth-promoting effect on corn plants and further improve the growth condition of the corn plants. The growth-promoting bacterium is pumilus minutus E2 and is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University in Wuhan, the preservation date is September 19, 2025, and the preservation number is CCTCC NO: M 20252071. By applying the strain to a corn culture medium, the bifurcation number, the root tip number, the root length, the dry weight, the fresh weight and the plant height of corn can be increased, the growth promoting effect of the strain on corn plants is proved, and a basic support is provided for further application to production.

The invention discloses a populus euphratica PeXTH7 gene and application of the populus euphratica PeXTH7 gene in improving drought resistance of plants, and belongs to the technical field of gene engineering. The CDS sequence of the populus euphratica PeXTH7 gene is disclosed for the first time and is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2; the effect of the populus euphratica PeXTH7 gene in improving the drought resistance of the plant is disclosed for the first time, the root length and lateral root density of the plant are remarkably improved through overexpression of the populus euphratica PeXTH7 gene, and the drought resistance of the plant is improved by adjusting the root structure of the plant and promoting root development; the populus euphratica PeXTH7 gene provided by the invention can be used as a candidate gene for breeding the drought-resistant new variety of the plant, and is of great significance for cultivating the drought-resistant new variety of the plant by adopting a genetic engineering method, accelerating the breeding process and reducing the breeding workload.

The invention discloses a tissue culture medium for rapid seedling raising of apple rootstocks and a rapid seedling raising method, and belongs to the technical field of plant tissue culture. The method specifically comprises the following steps: (1) obtaining a sterile material; (2) induction culture: inoculating the treated explants into an induction culture medium, and culturing at room temperature for 15-20 days to obtain adventitious buds; (3) multiplication culture: inoculating the adventitious buds into a multiplication culture medium, and carrying out light-dark culture at room temperature for 25-30 days to obtain cluster buds; (4) rooting culture: inoculating the cluster buds into a rooting culture medium, and carrying out light-dark culture at room temperature until the root length is 2-4cm; (5) transplanting. Through cooperation of the specific induction culture medium, the proliferation culture medium and the rooting culture medium, the growth and development rate and the survival rate of the apple rootstock can be effectively increased.

The present invention discloses the application of Mn3O4 nanofertilizer in alleviating the inhibition of plant growth by microplastic particles. The present invention finds that Mn3O4 can alleviate the stress of PS on the root length and fresh weight of Arabidopsis thaliana, and promote the growth of Arabidopsis thaliana. In addition, when Fv / Fm is 0.7, Arabidopsis thaliana is considered to be stress-free. The present invention finds that PS exerts stress on Arabidopsis thaliana, and Mn3O4 has the ability to alleviate PS stress. According to the chlorophyll fluorescence-related parameters provided by the present invention, it is proved that Mn3O4 alleviates the photosynthesis of Arabidopsis thaliana under PS stress, and 100-200μg / mL of Mn3O4 has the best effect on alleviating PS stress. Therefore, the Mn3O4 nanofertilizer provided by the present invention has a mitigating effect on the root length and photosynthesis of Arabidopsis thaliana under PS stress, thereby significantly promoting the growth and development of plants.

The invention discloses application of a BnaA06. TINY gene, a primer pair and a recombinant vector. The gene is used for improving the drought resistance of brassica napus. The gene can significantly enhance the drought resistance of brassica napus by increasing the root length and root-shoot ratio of brassica napus plants, reducing the accumulation of H2O2 and superoxide ions in leaves, reducing the MDA content and increasing the content of soluble sugar, proline and soluble protein in the leaves. The invention also provides a specific primer pair for amplifying the gene and a recombinant vector containing the gene, and the specific primer pair can be used for genetic transformation of the brassica napus so as to improve the drought resistance of the brassica napus. Through an agrobacterium tumefaciens-mediated transformation method, the gene is introduced into brassica napus cells and is expressed, so that the viability of brassica napus under drought conditions can be effectively improved, and a new gene resource and an effective method are provided for cultivating a drought-resistant brassica napus variety.

The invention relates to Cupriavidus sp. X3 and synbiotics with a plant growth promoting function and application of the Cupriavidus sp. X3 and the synbiotics, the Cupriavidus sp. X3 is named as a Cupriavidus sp. X3 strain, the preservation number is GDMCC No: 65855, and the preservation date is January 24, 2025. A new Cupriavidus sp. X3 with a plant growth promoting function is separated from cultivated land soil in Dongchuan mining areas in Yunnan province and is preserved, the strain has an excellent plant growth promoting function, the root length, plant height, root weight and plant weight of plants, especially corn and tobacco, can be improved, and crop growth promoting probiotic resources are enriched.

The invention belongs to the technical field of forest tree genetic engineering breeding, and particularly relates to a CbuLBD19 gene for regulating and controlling root system development and nitrogen absorption and utilization of catalpa bungei and application of the CbuLBD19 gene. The nucleotide sequence of the CbuLBD19 gene is as shown in SEQ ID No.7, and the amino acid sequence coded by the CbuLBD19 gene is as shown in SEQ ID No.8. It is found that overexpression of the CbuLBD19 gene can cause reduction of the total root length and the root biomass of catalpa bungei. Meanwhile, the net absorption rate of nitrate ions in the root system, the activity of nitrate reductase (NR) and the content of amino acid are reduced, and the comprehensive expression is that nitrogen metabolism is reduced. The negative regulation and control function of the CbuLBD19 gene on the nitrogen absorption and utilization of the catalpa bungei is defined, and a technical route for improving the nitrogen absorption and utilization of the catalpa bungei by reducing the expression of the CbuLBD19 gene is provided.

The invention belongs to the technical field of plant tissue culture, and relates to a tissue culture and rapid propagation method for castanopsis sclerophylla. According to the method, castanopsis sclerophylla seeds are used as materials, and through sterilization method comparison, basic culture medium screening, hormone proportioning optimization and the like, an efficient tissue culture and rapid propagation technical system of castanopsis sclerophylla is established through seed sterile germination, bud proliferation, strong bud rooting and acclimatization and transplantation, and complete tissue culture progeny plants are formed. 100.0% of the seeds in the system germinate and sprout; the propagation coefficient of buds is large and reaches 6.8; the rooting rate reaches 92.8%, the root system is developed, the average is 4.1 roots per plant, the root length is 4.6 cm, and lateral roots are dense; the transplanting survival rate is high and can reach 95.2% or above; and the method is not limited by factors such as seasons, climate and regions, and can realize annual efficient and rapid propagation. According to the method, the blank of tissue culture and rapid propagation of the castanopsis sclerophylla is filled, a stable and efficient regeneration method for the in-vitro culture plant of the castanopsis sclerophylla is provided, large-scale seedling culture can be carried out, the cultured castanopsis sclerophylla seedlings are robust, neat and consistent, and a high-quality seedling guarantee is provided for improving the economic benefit of an artificial forest of the castanopsis sclerophylla.

The present application relates to the field of plant growth and development molecular biology, and particularly relates to a gene OsARF14b and application thereof in controlling rice root elongation and tillering, the gene OsARF14b has the nucleotide sequence shown in SEQ ID NO:1, and the application of the gene OsARF14b in regulating rice root elongation and tillering traits.The present application obtains overexpression and knockout transgenic materials by cloning the gene OsARF14b, constructing a transgenic vector, and statistically analyzing the phenotype of the transgenic materials, and finds that the root length and tiller number in different materials change significantly, compared with the wild type (Zhonghua 11, ZH11), the root length of the knockout transgenic material (cas9) is significantly shortened, and the tiller number is significantly increased, the root length of the overexpression material (OE) is lengthened, and the tiller number is significantly reduced, which indicates that the OsARF14b gene plays an important regulatory function in the process of plant root elongation and tillering.

The invention relates to the technical field of plant tissue culture, in particular to a culture medium for asexual propagation of opisthopapus taihangensis and application of the culture medium. According to the method, the most suitable culture medium components in each stage of in-vitro rapid propagation of the opisthopapus taihangensis are studied, and a key technology for each link of in-vitro rapid propagation of the opisthopapus taihangensis is determined, namely, leaves of an opisthopapus taihangensis plant are used as test materials and are soaked in 75% alcohol for 40 seconds and soaked in 0.1% mercury bichloride for 6 minutes for sterile treatment; and then carrying out callus culture, adventitious bud culture, multiplication culture and rooting culture by adopting a specific culture medium to finally achieve the effects that the rooting rate is 100%, the average roots are 5.61, the average root length is 1.67 cm, the biomass fresh weight of the overground part is 0.14 g, and the biomass fresh weight of the underground part is 0.09 g. The invention provides thinking and technical support for germplasm conservation of opisthopapus longissima, and provides a basic material for research of medicinal value of opisthopapus longissima.

The invention provides a method for preventing and controlling plant aphids, which comprises the following specific steps: selecting plant germinated seeds with roots and root length of 2-6 cm, and in the growth process of the plant germinated seeds, applying light for 8 hours and photoperiod treatment in a dark cycle mode for 16 hours; in the plant germinated seeds, the seed sources comprise beans and cruciferous vegetables; meanwhile, in the growth process of the germinated seeds of the plants, a boron-containing nutrient solution is prepared from the following components: 4mM of Ca (NO3) 2.4 H2O, 1mM of KH2PO4, 1mM of KCl, 2mM of MgSO4. 7H2O, 0.5 mM of K2SO4, 50mu M of FeSO4. 7H2O, 50mu M of EDTA.Na2, 10mu M of MnCl2, 5mu M of ZnSO4. 7H2O, 0.5 mu M of CuSO4. 5H2O, 1mu M of (NH4) 6Mo7O24. 4H2O, 35-70 mu M of H3BO3 and 0.5 mu M of CoCl2; nutrient elements are applied according to the following steps that (1) a first boron-containing nutrient solution is adopted in 1-5 days, and the concentration of all components in the first boron-containing nutrient solution is 20-30% of that of the boron-containing nutrient solution; and (2) after 6-15 days, adopting a second boron-containing nutrient solution, wherein the concentration of all components in the second boron-containing nutrient solution is 40-60% of that of the boron-containing nutrient solution. The method can effectively prevent and control plant aphids.

The invention discloses a method for promoting strawberry root system development by using K599 agrobacterium rhizogenes. The method comprises an assembly type elevated cultivation device, seedling cultivation management and a method for promoting root system development. And the overhead cultivation device is adopted, so that the problems of gray mold and the like caused by field cultivation can be reduced. Seedling cultivation management is used for helping propagation of strawberry resources, reserving variety characteristics and shortening the seedling period. And promoting root development to help strawberry seedlings to rapidly root, strengthen roots, strengthen seedlings in a short period of time and shorten the seedling raising period. Efficient cultivation management and a rooting promoting method are combined, and the propagation period of the strawberries is shortened. According to the method, the K599 agrobacterium rhizogenes are used for promoting rooting of strawberry seedlings and absorption of plant nutrient substances by root systems, the root length, the root tip number and the root specific surface area are remarkably increased by 19.85%, 39.63% and 68.79% respectively, and the root systems are developed and robust. The strawberry seedling strengthening time can be shortened, cost is low, operation is easy and convenient, and the effect is remarkable.

The application provides a strain of protozoan nephrospora NJAU-HN1 and an application thereof, and belongs to the technical field of agricultural microorganisms. The nephrospora NJAU-HN1 has a preservation number of CCTCC NO: C2025172. The nephrospora NJAU-HN1 can directly and effectively inhibit the survival of root-knot nematodes. Application of the nephrospora NJAU-HN1 or a culture thereof can significantly promote the growth of melon plants, increase the fresh weight, dry weight and root length of the plants. The nephrospora NJAU-HN1 provided by the application provides valuable microbial resources for the development of a new microbial preparation for green prevention and control of melon root-knot nematode disease and crop health promotion, and has a good application prospect.

The invention discloses a microbial composite agent and its application, and relates to the field of microbial technology. The microbial composite agent disclosed in the invention is composed of bradyrhizobium WP5 and sphingobacterium WD6. The results show that compared with no inoculation, after inoculation with the microbial composite agent of the present application, the height of soybean plants is significantly increased, the number of nodules is significantly increased, and the root system is more developed and dense; the fresh weight of the aboveground part of soybeans increased by 85.92%; the fresh weight of the underground part of soybeans increased by 59.17%; the fresh weight of soybean nodules increased by 58.83%; the height of soybeans increased by 19.35%; the thickness of soybean stems increased by 17.60%; the total root length increased by 21.58%; the number of root tips increased by 69.88%; the number of branches increased by 21.97%; and the root volume increased by 190.75%. In summary, the co-inoculation of bradyrhizobium WP5 and sphingobacterium WD6 has a significant promoting effect on the growth and development of soybeans.

The invention discloses application of OsPL3 protein in regulation and control of salt tolerance and root length of rice. The invention belongs to the technical field of biology, and particularly relates to application of OsPL3 protein to regulation and control of salt tolerance and root length of rice. The protein provided by the invention can positively regulate and control the salt tolerance and root length of rice, through the expression quantity of the overexpressed OsPL3 protein, after salt stress treatment, the survival rate of seedlings of the obtained overexpressed plant OsPL3-OE is obviously increased by about one time compared with that of a wild type, the root length development of the OsPL3-OE is also obviously longer than that of the wild type, and the protein has a certain application prospect for rice breeding.

The invention discloses a method for remarkably improving the rooting rate of clustered buds of citral-type camphor trees, which comprises the following steps: cutting a 2-3cm stem section with a stem tip, and transferring the stem section into a pre-culture medium; transferring into a rooting culture medium; adding plant hormones, observing the growth condition of the adventitious roots, and counting the inductivity, the average root length and the average root number of the adventitious roots. By means of the technology of anti-oxidation treatment on clumpy bud incisions, the technology of culture medium improvement, culture condition optimization, the technology of adding a plant root tip tissue extracting solution and the like, plant tissue browning is effectively reduced, the rooting rate of the citral-type camphor tree is increased, the rooting culture process of the citral-type camphor tree tissue culture seedlings is remarkably improved, and the rooting rate of the citral-type camphor tree tissue culture seedlings is increased. The citral-type camphor tree cluster bud rooting seedling with a developed and strong root system is obtained, a technical support is provided for conservation and propagation of the tree species, the quality of the seedling is ensured, the resource basis and quality of the citral-type camphor tree are improved, and healthy and sustainable development of the citral-type camphor tree and the natural citral industry is promoted.