The invention belongs to the technical field of technologies for growth media for edible mushrooms and particularly relates to a production strain growth medium for Volvariella volvacea and a preparation method of the production strain growth medium. A grain growth medium is usually used for cultivating the Volvariella volvacea in the prior art and has the disadvantages that the cost is high, the growth medium is not dispersed easily after sterilization, stock seeds are prone to aging and the storage life is short. According to the growth medium for the Volvariella volvacea and the preparation method of the growth medium, corncob is taken as a main raw material, bran, cow dung, cottonseed hulls, soybean meal and other materials are added, and the growth medium is prepared through lactic acid fermentation. Agricultural by-products such as the corncob, the cow dung, the cottonseed hulls and the like are used sufficiently, the cost is saved, the growth medium is dispersed easily after sterilization, the strain has long storage life, germination and uptake are fast after inoculation, and the final fruiting conversion rate is high; besides, rodent damage can be eliminated during fruiting, use of rodenticides is avoided, the safety of the Volvariella volvacea is guaranteed, and environmental damage is avoided.