The invention discloses a biogas slurry fattening turtle feed and a preparation method thereof. The biogas slurry fattening turtle feed is prepared from, by weight, 300-400 parts of pig hair meal, 200-300 parts of fly maggot, 70-80 parts of highland barley, 30-40 parts of inkfish paste, 20-30 parts of biogas slurry, 80-90 parts of bean pulp, 70-80 parts of maize germ meal, 10-20 parts of a mixture of rice chaff and husks, 10-20 parts of malt sprout, 8-15 parts of pumpkin seeds, 40-50 parts of grassiness, 30-40 parts of teosinte, 2-3 parts of honeysuckle, 1-2 parts of radix sophorae flavescentis, 2-3 parts of dandelion, 2-3 parts of coptis chinensis, 50-60 parts of garlic husks, 20-30 parts of apple juice, 15-20 parts of lentinus edodes powder, 5-8 parts of maize pollen, 2-3 parts of oleum morrhuae, 3-4 parts of egg shell powder, 2-3 parts of vinegar powder and 0.5-1 part of vanilla powder. The feed is comprehensive in nutrient, delicious, convenient to use and remarkable in benefit, turtles can growth rapidly, and are beautiful in appearance, healthy and easy to fatten, time and materials are saved, and turtle meat is delicious and rich in nutrient.