181 results about "Vaginitis" patented technology

The invention relates to the field of microorganisms, and discloses lactobacillus plantarum and application of the lactobacillus plantarum in preparation of a medicine for preventing and / or treating vaginitis, and the preservation number of the lactobacillus plantarum is CGMCC No.33152. The lactobacillus plantarum provided by the invention has a relatively strong bacteriostatic effect on common vaginal pathogenic bacteria such as Atortoria, staphylococcus aureus, Candida albicans, Escherichia coli and Gardneria, and also can reduce the infection degree of the pathogenic bacteria by inhibiting colonization and reproduction of the pathogenic bacteria; in addition, the proinflammatory factor level in sick vaginitis tissues is reduced, and the anti-inflammatory factor level is improved.

The invention relates to lactobacillus crispatus LC30 for preventing and treating female genital tract infection and application of the lactobacillus crispatus LC30. The lactobacillus crispatus LC30 is preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC NO.31923. The lactobacillus crispatus LC30 has good hydrogen peroxide production capacity and adhesion capacity, has a strong inhibition effect on main vaginitis pathogenic bacteria, and can be used for preventing and treating female genital tract infection. The composition is effective to nutritional pathogenic bacteria, and can also reduce form transformation and biological membrane formation of the pathogenic bacteria and prevent and treat drug resistance; besides, the fermentation product can reduce the content of pathogenic bacteria metabolites, regulate the secretion of cytokines and achieve the effect of relieving inflammation, so that the fermentation product can be used for preparing products for preventing and treating genital tract pathogenic bacteria infection, preventing and treating vaginitis and / or removing pudendum peculiar smell.

The invention relates to lactobacillus gasseri FMTA1125B with the effect of preventing, relieving or treating vaginitis and application of the lactobacillus gasseri FMTA1125B. The lactobacillus gasseri FMTA1125B is classified and named as lactobacillus gasseri, and the preservation number of the lactobacillus gasseri FMTA1125B is CGMCC No.34292. The lactobacillus gasseri FMTA1125B has the effects of preventing, relieving or treating vaginitis and the application of the lactobacillus gasseri FMTA1125B. The bacterium can produce beneficial components such as lactic acid, gamma-aminobutyric acid and hydrogen peroxide through metabolism so as to regulate the genital tract environment and improve the health degree; and the traditional Chinese medicine composition has relatively good bacteriostasis and sterilization effects on common vaginal pathogenic bacteria such as escherichia coli, staphylococcus aureus, gardnerella vaginalis, candida albicans, neisseria gonorrhoeae and the like. Therefore, the lactobacillus gasseri FMTA1125B strain can be used for preparing a preparation with the effect of improving related genital tract diseases such as vaginitis.

The invention discloses a primer and a method for detecting bacterial vaginitis related pathogens and application of the primer and the method. The nucleic acid sequence of the primer group comprises the sequences as shown in SEQ ID NO.1 to SEQ ID NO.12. The invention further discloses a kit for detecting the content of the nucleic acid sequence. The invention provides a primer group and a method for evaluating bacterial vaginitis related pathogens by designing specific primers to carry out super-multiplex PCR amplification and enrichment, obtaining the detection data volume of each pathogen based on sequence reading of a gene sequencing platform and assisting by a machine learning model, and further provides a kit for evaluating bacterial vaginitis related pathogens and a kit for evaluating bacterial vaginitis related pathogens. The problems that an existing Amsel and Nutant scoring method is greatly influenced by subjective factors, the requirement for the sample size of multiple qPCR joint detection is high, and the 16s rDNA detection cost is high can be solved.

The present invention relates to the screening of functional probiotics and its application, and specifically provides a strain of Lactobacillus casei RH99 that produces high extracellular polysaccharides and relieves vaginitis, and its preservation number is CGMCC No.32108. The Lactobacillus casei can inhibit the growth of Gardnerella, Candida albicans, and Peptostreptococcus and their biofilm generation ability; the extracellular polysaccharides produced by it have a significant promoting effect on the biofilm formation of Lactobacillus casei itself. RH99 is inoculated into the vagina, and its strain, the metabolites (exocellular polysaccharides) produced, and the core monosaccharide (D-mannitol) can all inhibit the colonization of pathogenic bacteria and promote the colonization of lactic acid bacteria; and RH99 can also relieve vaginitis by inhibiting inflammatory factors. It can be seen that RH99 can inhibit the growth of pathogenic bacteria + inhibit biofilm formation + inhibit pathogenic bacteria adhesion + inhibit the level of inflammatory factors through key core metabolites, and realize the all-round inhibition of four-in-one.

The present invention discloses a strain of Lactobacillus paracasei that simultaneously activates immune regulation and antioxidant pathways to alleviate vaginitis, belonging to the field of microbial technology. The present invention screened and obtained a strain of Lactobacillus paracasei CCFM1316, which has the effect of repairing related symptoms such as immune disorders and oxidative damage caused by vaginitis, specifically embodied in: reducing cellular NF-κB mRNA expression and cell supernatant IL-1β content, improving mouse vaginal immune response, enhancing mouse vaginal antioxidant capacity, improving mouse vaginal pathological manifestations and reducing pathogenic bacteria load. It reduces the mouse vaginal Gardnerella load and improves mouse vaginal pathological manifestations; therefore, Lactobacillus paracasei CCFM1316 has great application prospects in products that improve and repair immune disorders and oxidative damage problems caused by vaginitis.

The invention relates to the technical field of microorganisms, in particular to lactobacillus rhamnosus HZBX-05 and application thereof.The lactobacillus rhamnosus HZBX-05 is sent to the China General Microbiological Culture Collection Center on July 27th, 2023 to be preserved in the China General Microbiological Culture Collection Center, the preservation number of the lactobacillus rhamnosus HZBX-05 is CGMCC NO.987, and the preservation number of the lactobacillus rhamnosus HZBX-05 is CGMCC NO.989. The lactobacillus rhamnosus HZBX-05 is CGMCC NO.989. The lactobacillus rhamnosus HZBX-05 is CGMCC NO.989. The preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.28035, and the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, West Beichen Road, Chaoyang District, Beijing; the separated Lactobacillus rhamnosus HZBX-05 is continuously treated for 7 hours in a simulated gastric acid cholate environment, and the result shows that the HZBX-05 still has high survival rate, has the capability of being highly adsorbed to intestinal cells Caco-2 cells, has the capability of inhibiting various pathogenic bacteria, has immunoregulation and anti-inflammation effects, has the effect of resisting helicobacter pylori, and can be used for preparing an anti-inflammatory drug. The symptoms of female vaginitis can be improved.

The invention discloses lactobacillus crispatus for improving female genital tract microenvironment and immune aging and application of the lactobacillus crispatus, and belongs to the field of probiotics. The probiotic lactobacillus crispatus ProSci-38 disclosed by the invention can be used for effectively improving the symptoms such as pruritus vulvae, causalgia of vagina, abnormal vaginal secretion and the like of patients with vaginitis; mainly through the effect of intestinal tract-vaginal axis, the proportion of lactobacillus is increased, the proportion of harmful bacteria is reduced, the balance of vaginal flora is recovered, and the acidic environment of vagina is maintained; meanwhile, the inflammatory factor level can be reduced, the immune function of the vagina of a patient can be improved, so that the vagina environment is improved, the infection risk is reduced, and the preparation method has a great prospect in preparation of products for relieving and / or treating vaginitis.

The invention relates to lactobacillus gasseri FMTA1121E with an effect of regulating a vaginal micro-ecological environment and application of the lactobacillus gasseri FMTA1121E. The lactobacillus gasseri FMTA1121E is classified and named as lactobacillus gasseri, and the preservation number of the lactobacillus gasseri FMTA1121E is CGMCC No.34962. The lactobacillus gasseri FMTA1121E can be metabolized to generate beneficial components such as lactic acid, gamma-aminobutyric acid and hydrogen peroxide so as to regulate the genital tract environment and improve the health degree; and the bacillus subtilis has excellent bacteriostasis and sterilization effects on various common vaginal pathogenic bacteria such as escherichia coli, staphylococcus aureus, gardnerella vaginalis, candida albicans, neisseria gonorrhoeae and the like. Therefore, the lactobacillus gasseri FMTA1121E strain can be applied to preparation of preparations for preventing and treating related genital tract diseases such as vaginitis.

The invention relates to combined lactobacillus salivarius LS100 for inhibiting vaginitis pathogenic bacteria and preventing and treating vaginitis and application of the combined lactobacillus salivarius LS100. The combined lactobacillus salivarius LS100 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.32135. The combined lactobacillus salivarius LS100 has good hydrogen peroxide production capacity and adhesion capacity, and can be used for preparing medicines for preventing and treating vaginitis. The traditional Chinese medicine composition has a relatively strong inhibition effect on main vaginitis pathogenic bacteria, is effective on nutritional pathogenic bacteria, and can also reduce form transformation and biological membrane formation of the pathogenic bacteria and prevent generation of drug resistance; besides, the fermentation product can reduce the content of pathogenic bacteria metabolites, regulate the secretion of cell factors and play a role in relieving inflammation, so that the fermentation product can be used for preparing products for preventing and treating genital tract pathogenic bacteria infection and / or preventing and treating vaginitis.

The invention relates to the technical field of biological medicine, in particular to # imgabs0 gynecological antibacterial gel for improving inflammatory response of vaginitis and vagina environment and application of the # imgabs0 gynecological antibacterial gel. The invention provides a postbiotic composition. The postbiotic composition comprises inactivated thalli of a probiotic composition and metabolites of the inactivated thalli; the probiotic composition is prepared from lactobacillus paracasei (Lactobacillus paracasei) ProSci-92 and lactobacillus rhamnosus ProSci-109, and the probiotic composition is prepared from lactobacillus paracasei (Lactobacillus paracasei) ProSci-92, lactobacillus rhamnosus (Lactobacillus rhamnosus) ProSci-109, lactobacillus rhamnosus (Lactobacillus rhamnosus) ProSci-109 and lactobacillus rhamnosus (Lactobacillus rhamnosus) ProSci-109. The preservation number of the lactobacillus paracasei ProSci-92 is CGMCC (China General Microbiological Culture Collection Center) No.26526; the preservation number of the lactobacillus rhamnosus ProSci-109 is CGMCC (China General Microbiological Culture Collection Center) No. 25449; the postbiotic composition can effectively slow down or treat inflammatory response of vaginitis and improve the vagina environment.

The invention discloses probiotics with an effect of improving vaginitis and a composite microecological preparation and application of the probiotics. The probiotics are characterized by comprising at least one of a plant lactobacillus 22-9 strain with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.33867, a plant lactobacillus XA-9 strain with the preservation number of CGMCC No.33868 and a plant lactobacillus pentosus B-1-35 strain with the preservation number of CGMCC No.33870, the preparation method comprises the following steps: inoculating a lactic acid bacteria starter, which is prepared by mixing lactic acid bacteria cultures of plant lactobacillus 22-9, plant lactobacillus XA-9 and plant lactobacillus pentosus B-1-35 according to a volume ratio of 2: 1: 3, into a fermentation culture medium according to a volume ratio of 1-5%, so as to prepare the probiotic compound microecological preparation. The number of inflammatory cells in the vagina is reduced, and the levels of inflammatory factors IL-1beta, IL-6 and TNF-alpha in serum are reduced.

The invention belongs to the technical field of female private part nursing, and particularly relates to andrographolide gel as well as a preparation method and application thereof. The invention provides an andrographolide gel, which is prepared from the following components in parts by mass: 0.1 to 0.5 part of andrographolide, 2.0 parts of caprylic / capric polyethylene glycol glyceride, 5.0 parts of propylene glycol, 0.05 to 0.2 part of chitosan, 10.0 parts of 1 percent W / V lactic acid solution, 0.15 to 0.6 part of hydroxyethyl cellulose and 30.0 parts of water. The andrographolide, the caprylic / capric polyethylene glycol glyceride, the propylene glycol, the chitosan, the 1% W / V lactic acid solution, the hydroxyethyl cellulose and the water are combined for use, the proportion of the components is strictly limited, and the obtained andrographolide gel can effectively inhibit vaginal pathogenic bacteria (including candida chromosomes, escherichia coli, staphylococcus aureus and gardnerella); the traditional Chinese medicine is used for treating fungal vaginitis and has no irritation to vaginal tissues.

The invention provides weak acid wet toilet paper special for women and a preparation process thereof, and belongs to the technical field of wet toilet paper preparation. The preparation process comprises the following steps: preparing the plant source weak acidity adjusting compound; preparing a bacteriostatic microcapsule compound; and preparing the weakly acidic wet toilet paper. The weakly acidic wet toilet paper prepared by the invention can relieve redness and pruritus of private skin caused by friction and secretion stimulation and enhance the skin barrier function, and an'instant bacteriostasis-long-acting regulation 'dual path is formed by the bacteriostatic component released by the microcapsule, the cranberry proanthocyanidin and the lactic acid bacteria metabolite, so that the recurrence rate of vaginitis is reduced.

The invention discloses an olive leaf extract-containing antibacterial composition and a preparation method and application thereof.The preparation method comprises the following steps: taking ferroferric oxide particles wrapped by oleic acid as a magnetic source, preparing magnetic polymer microspheres by a microemulsion method, coating the surfaces of the magnetic microspheres with silicon dioxide by a surface chemical coating modification method, and then performing amination modification to obtain the olive leaf extract-containing antibacterial composition. The obtained aminated magnetic microspheres are used for separating and purifying bacteriostatic active ingredients in a mixed solution with olive leaf extract as a main material and extracts of radix sophorae flavescentis, cortex phellodendri, fructus cnidii, liquorice, radix isatidis, honeysuckle, aloe and the like as auxiliary materials, and after desorption, a desorption solution is subjected to rotary evaporation concentration and vacuum drying; and compounding with several of nicotinamide, coenzyme Q10, nootkatone, sweet osmanthus hydrolat, rose hydrolat, antibacterial peptide and the like to develop a novel product for preventing and treating vulvovaginitis. The composition not only can resist bacteria synergistically, but also has small influence on lactobacillus, does not cause flora imbalance, and can effectively delay the generation of antibiotic resistance of bacteria.

The invention belongs to the technical field of preparation and application of probiotic postbiotics, and particularly relates to a composite probiotic postbiotic composition and application thereof. According to the compound probiotic metagen composition disclosed by the invention, the four probiotic metagens are compounded, so that vaginal harmful bacteria including bacteria and molds can be effectively inhibited, and the compound probiotic metagen composition has good in-vitro antibacterial activity; on the basis, the prebiotics are added into the composite probiotic postbiotic composition, so that the growth and stability of in-vivo lactobacillus inheritance can be promoted, the balance of vaginal flora can be recovered, and the diversity of species in microbial communities can be remarkably increased; meanwhile, symptoms such as vagina external redness and swelling, bleeding and pruritus caused by vaginitis can be effectively improved, no allergy phenomenon is generated, and therefore the compound can be applied to preparation of preparations for preventing and / or relieving vaginitis and can also be applied to production of antibacterial hygienic products, especially female hygienic products.

The invention provides a probiotic composition for treating vaginitis and a preparation method thereof, and belongs to the technical field of probiotics. The preparation method comprises the following steps: performing water extraction on cortex phellodendri, honeysuckle and cacumen biotae to obtain an extract, inoculating probiotics into medicine residues for fermentation, performing separation to obtain antibacterial peptide, bacterial sludge and a fermentation product, preparing an antibacterial peptide-polysaccharide compound from the antibacterial peptide, embedding the bacterial sludge through an acid-soluble shell layer to obtain an acid-soluble bacterium-loaded microcapsule, mixing the acid-soluble bacterium-loaded microcapsule with the antibacterial peptide-polysaccharide compound, the extract and prebiotics, adding the mixture into hydrogel, and performing freeze-drying to obtain a finished product. And preparing the probiotic composition for treating vaginitis. The prepared probiotic composition for treating vaginitis is good in treatment effect, good in storage stability, good in temperature resistance and moisture resistance, safe to use, free of side effects, good in biocompatibility, free of drug resistance, beneficial to treatment and prevention of relapse of vaginitis and wide in application prospect.

The invention relates to combined lactobacillus salivarius LS360 for preventing and / or improving female genital tract infection and application of the combined lactobacillus salivarius LS360. The combined lactobacillus salivarius LS360 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.32134, and the combined lactobacillus salivarius LS360 has good biological characteristics and endurance capacity and can be used for preventing and / or improving female genital tract infection. The traditional Chinese medicine composition has a relatively strong inhibition effect on main vaginitis pathogenic bacteria, is effective on nutritional pathogenic bacteria, and can also reduce form transformation and biological membrane formation of the nutritional pathogenic bacteria and prevent and treat drug resistance; besides, the bacterial strain and the fermentation product can also reduce the content of pathogenic bacteria metabolites, regulate the secretion of cell factors and play a role in relieving inflammation, so that the bacterial strain and the fermentation product can be used for preparing products for preventing and treating female genital tract pathogenic bacteria infection, preventing and treating vaginitis and / or removing pudendum peculiar smell.

The invention discloses a lactobacillus acidophilus FMM01 with the effects of improving the contractility of pelvic floor muscles and repairing mucous membranes and application of the lactobacillus acidophilus FMM01, the lactobacillus acidophilus FMM01 is preserved in the China Center for Type Culture Collection, the preservation date is January 9, 2024, and the preservation number is CCTCC NO: M2024052. The lactobacillus acidophilus FMM01 has the advantages of self-adaption, high acid resistance, strong mucous membrane adhesiveness and no irritation to vaginal mucosa. The lyophilized powder is high in activity and excellent in stability; experimental results show that the lactobacillus acidophilus FMM01 can effectively inhibit bacteria, and especially can effectively inhibit enterobacter cloacae, vibrio parahaemolyticus and / or candida albicans. Besides, the lactobacillus acidophilus FMM01 can improve vaginal relaxation, improve pelvic floor muscle contractility and / or recover vaginal mucosa functions, can promote recovery of healthy florae after mixed vaginitis antibiotic / hormone treatment, improves the treatment effect of mixed vaginitis, and remarkably reduces the recurrence rate of mixed vaginitis.

The invention relates to the technical field of biology, in particular to leukocyte esterase detection test paper and a preparation method thereof. Based on the principle of a substrate and a color developing agent, the color developing depth is in direct proportion to the concentration of the esterase, so that the concentration of the leukocyte esterase in a detection sample can be judged according to the color developing depth; meanwhile, under the auxiliary action of calcium chloride and other materials, the detection distinction degree, the detection accuracy and the detection result stability are further improved. The leukocyte esterase test paper provided by the invention is simple in sample detection method, more stable in product performance, longer in validity period and wide in application range, can be used as an auxiliary evaluation index for vagina inflammation, and is combined with a cleanliness index and the like to jointly evaluate the vagina environment.

The invention provides a novel application of L-beta-galactoglucan, and particularly provides an application of the L-beta-galactoglucan in the aspect of resisting vaginitis.

The application discloses an active oxygen response type bacteriostatic and repair promoting hydrogel and a preparation method and application thereof, the hydrogel is formed by disulfide bond cross-linked hydrogel in response to active oxygen free radicals in the vagina of vaginitis patients through thiol modified hyaluronic acid (HASH), and can enhance in-vivo retention by thiol and vaginal tissue interaction. Cationic surfactant such as didecyldimethylammonium chloride (DDAC) as the bacteriostatic component of the hydrogel, efficiently killing pathogenic bacteria while having good biocompatibility, and is not prone to cause bacterial drug resistance. As a natural immune regulator, beta-glucan regulates the vaginal microenvironment, promotes the polarization of macrophages into anti-inflammatory M2 type and promotes cell migration, etc., to promote the repair of the vaginal immune barrier, regulate the vaginal flora, and reduce the probability of vaginitis recurrence. The HASH hydrogel provides an efficient, convenient and highly transformative potential strategy to provide more choices for the treatment of vaginitis.

The application belongs to the technical field of microorganisms, and particularly relates to two strains of lactobacillus plantarum PB12 and PB14 and application thereof in preventing or treating female vaginitis. lactiplantibacillus plantarum The application discloses two strains of lactobacillus plantarum (Lactobacillus plantarum), namely lactobacillus plantarum PB12 and lactobacillus plantarum PB14. The application also discloses application of the two strains of lactobacillus plantarum in preparing medicines or products for preventing and / or treating female vaginitis, including inhibiting growth of Candida albicans and relieving inflammation of a genital tract.

To provide compositions and methods for detecting bacterial nucleic acids and diagnosing bacterial vaginosis.SOLUTION: Disclosed is a method for diagnosing bacterial vaginosis in a subject comprising performing an assay to detect one or more of Lactobacillus spp., Atopobiumvaginae, and Gardnerallvaginalis in a sample of the subject. Also disclosed are compositions and methods for detecting Lactobacillus spp., Atopobiumvaginae, and / or Gardnerallvaginalis nucleic acids in a sample.SELECTED DRAWING: None

The invention relates to the field of prevention and treatment of vaginitis, in particular to a composition of lactobacillus rhamnosus LRa05, lactobacillus acidophilus La88 and lactobacillus plantarum Lp90 and application of the composition to regulation of vaginal mucosa barrier protein. The lactobacillus rhamnosus LRa05 is a lactobacillus rhamnosus LRa05 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.24377, and the lactobacillus rhamnosus LRa05 is a lactobacillus rhamnosus LRa05 with the preservation number of CGMCC NO.24377. The lactobacillus acidophilus La88 is the lactobacillus acidophilus La88 of which the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.24109, and the lactobacillus acidophilus La88 is a lactobacillus acidophilus La88 of which the preservation number is CGMCC NO.24109. The lactobacillus plantarum Lp90 is the lactobacillus plantarum Lp90 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.10453, and the lactobacillus plantarum Lp90 has the preservation number of CGMCC NO.10453. The lactobacillus rhamnosus LRa05, the lactobacillus acidophilus La88, the lactobacillus plantarum Lp90 and the combination thereof disclosed by the invention can regulate the expression of the vagina mucosal barrier protein.

The invention relates to fermented lactobacillus mucus FMTA1124J with the function of improving the health degree of female genital tracts and application of the fermented lactobacillus mucus FMTA1124J. The classified name of the fermented lactobacillus mucus FMTA1124J is Limosilicilla fermentum, and the preservation number of the fermented lactobacillus mucus FMTA1124J is CGMCC No.34963. The fermented lactobacillus mucus FMTA1124J can produce beneficial components such as lactic acid, gamma-aminobutyric acid and hydrogen peroxide through metabolism so as to adjust the environment of the genital tracts and improve the health degree; and the traditional Chinese medicine composition has good bacteriostasis and sterilization effects on common vaginal pathogenic bacteria such as Escherichia coli, staphylococcus aureus, Gardneria vaginalis and Candida albicans. Therefore, the fermented lactobacillus mucus FMTA1124J strain can be used for preparing a preparation with the effect of improving related genital tract diseases such as vaginitis.

The invention relates to a metastatic composite fiber with a targeted antibacterial effect and a preparation method and application thereof.The metastatic composite fiber comprises a core layer, a skin layer and a metastatic microcapsule coating wrapping the skin layer, and the core layer is prepared from polyester and / or polypropylene; the preparation material of the skin layer comprises polyethylene and / or modified polyethylene, and the preparation raw materials of the metagen microcapsule coating comprise a metagen microcapsule and a hydrophilic oil agent. The prepared metastatic composite fiber has excellent mechanical properties and relatively high softness, also has an excellent effect of selectively inhibiting genital tract pathogenic bacteria, and can effectively improve the vagina environment of a user and relieve vagina inflammation when being applied to preparation of hygienic products; the micro-ecological balance of the vagina of the user is maintained; when applied to underwear and other close-fitting textile clothes, the fabric can effectively inhibit bacteria and maintain the stability of the micro-ecological environment of the skin surface layer.

The invention discloses a micro-ecological viable bacterium preparation as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) preparing a micro-nutrient solution; (2) pretreating a micro nutrient solution; (3) preparing target probiotic bacterial sludge; (4) preparing a micro-ecological viable bacteria solution; (5) adding a freeze-drying protective agent into the micro-ecological viable bacteria solution to prepare a micro-ecological viable bacteria solution before freeze-drying; and (6) freeze-drying the micro-ecological viable bacteria solution before freeze-drying to obtain the micro-ecological viable bacteria preparation. According to the method disclosed by the invention, nutritional ingredients for promoting the probiotics to inhibit pathogenic bacteria and the probiotics are jointly freeze-dried to prepare the micro-ecological living bacteria preparation, nutrients in the preparation can be used for the probiotics to rapidly proliferate after administration and generate functional substances so as to play a role, the antibacterial rate reaches 90% or above within 8 hours and reaches 100% within 24 hours, and the treatment effect is safely and rapidly achieved; meanwhile, the dependence of probiotics on environmental nutrition is reduced. The micro-ecological living bacteria preparation prepared by the process can be applied to prevention and treatment of gynecological genital tract inflammation such as colpitis mycotica.

The application discloses application of a traditional Chinese medicine composition in preparation of a medicine for treating vaginitis, and preparation raw materials of the traditional Chinese medicine composition include zedoary turmeric and Chinese atractylodes rhizome. The traditional Chinese medicine composition can kill common pathogenic bacteria of vaginosis, and has good bacteriostatic effect. While inhibiting the common pathogenic bacteria of vaginosis, the traditional Chinese medicine composition does not inhibit lactobacillus of vagina. The traditional Chinese medicine composition can not only kill single common pathogenic bacteria of vaginosis, but also relieve trichomonas vaginalis, and inhibit mixed infection of bacteria and fungi.

The L-theanine composition provided by the invention is placed at 60 DEG C for 10 days, the growth rate of pyroglutamic acid impurities is within 3 times, and the quality requirement that the content of the pyroglutamic acid impurities is below 1% is met; the L-theanine composition is prepared from L-theanine, an excipient and magnesium stearate, wherein the mass ratio of the L-theanine is 5%-30%; the excipient comprises one or more of pregelatinized starch, lactose, microcrystalline cellulose, mannitol, colloidal silicon dioxide and / or a pH regulator, and the mass ratio of the excipient is 65%-94.9%; the mass ratio of the magnesium stearate is 0.1%-5%. Therefore, the medicine is endowed with higher stability, so that the L-theanine preparation composition which is good in stability, effectiveness and safety and meets the pharmacopoeia standard is obtained. Meanwhile, related pharmacodynamic experiments are carried out by the inventor, and it is proved that the composition can be used for preparing the medicine for preventing and / or treating vaginitis.