Special weakly acidic wet toilet paper for women and preparation process thereof

Abstract

The invention provides weak acid wet toilet paper special for women and a preparation process thereof, and belongs to the technical field of wet toilet paper preparation. The preparation process comprises the following steps: preparing the plant source weak acidity adjusting compound; preparing a bacteriostatic microcapsule compound; and preparing the weakly acidic wet toilet paper. The weakly acidic wet toilet paper prepared by the invention can relieve redness and pruritus of private skin caused by friction and secretion stimulation and enhance the skin barrier function, and an'instant bacteriostasis-long-acting regulation 'dual path is formed by the bacteriostatic component released by the microcapsule, the cranberry proanthocyanidin and the lactic acid bacteria metabolite, so that the recurrence rate of vaginitis is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of wet toilet paper preparation, and specifically relates to a weakly acidic wet toilet paper for women and its preparation process. Background Art

[0002] With the improvement of women's health care awareness, the demand for special sanitary products for intimate area cleaning is increasing day by day. As an upgraded product of traditional toilet paper, wet toilet paper has gradually become an important choice for women's daily care due to its advantages such as gentle cleaning and reducing friction damage. However, the current wet toilet paper products on the market generally have the following technical problems and are difficult to fully meet the nursing needs of women's special physiological environment:

[0003] The normal pH value of women's intimate area usually maintains a weakly acidic environment of 3.8 - 4.5, which helps to inhibit the reproduction of harmful bacteria and maintain the balance of the flora. Most of the existing wet toilet papers do not specifically adjust the pH value, and the pH values of the products are mostly neutral or slightly alkaline. Long-term use may damage the microecology of the intimate area and increase the risk of gynecological inflammation. Some products claiming to be "weakly acidic" only adjust the pH value by adding chemical acid substances, lacking a scientific design of the acid-base buffer system, resulting in poor stability and easy to cause a sense of irritation during use.

[0004] Traditional wet toilet papers often use irritating ingredients such as alcohol, perfume, and preservatives to enhance the cleaning or antibacterial effect, but such ingredients are easy to damage the natural barrier of the intimate area, resulting in discomfort such as dryness and itching. Especially for women with sensitive skin, the mildness of existing products is insufficient to meet the daily cleaning and nursing needs during special physiological periods (such as menstrual periods and pregnancy). In addition, most products only focus on the basic cleaning function, lacking antibacterial and maintenance effects, and cannot reduce irritation and achieve a maintenance effect while cleaning, and inhibit the growth of harmful bacteria.

[0005] Therefore, we propose a weakly acidic wet toilet paper for women that can effectively antibacterial and its preparation process. Summary of the Invention

[0006] Aiming at the deficiencies of the existing technology, the purpose of the present invention is to provide a weakly acidic wet toilet paper for women and its preparation process.

[0007] A preparation process of a weakly acidic wet toilet paper for women includes the following steps:

[0008] S1: Preparation of a plant-derived weakly acidic regulating complex

[0009] Compound cranberry extract and lactic acid bacteria fermentation broth to prepare a plant-derived weakly acidic regulating complex;

[0010] S2: Preparation of an antibacterial microcapsule complex

[0011] The surface of nano-zinc oxide was modified with 3-aminopropyltriethoxysilane, and then it was mixed with ε-polylysine to prepare an ε-polylysine / nano-zinc oxide composite. Then, sodium alginate and the ε-polylysine / nano-zinc oxide composite were used as the aqueous phase, and targeted chitosan and liquid paraffin were used as the oil phase to prepare an antibacterial microcapsule composite;

[0012] S3: Preparation of weakly acidic wet toilet paper

[0013] A microcapsule suspension was prepared using glucosylglycerol, xanthan gum, octanohydroxamic acid, and the antibacterial microcapsule composite. Then, it was immersed in a treatment solution to obtain a doubly modified non-woven fabric substrate. A wetting system solution was prepared by mixing glucosylglycerol, octanohydroxamic acid, and a plant-derived weakly acidic regulating composite. Then, the doubly modified non-woven fabric substrate was impregnated in the wetting system solution to adjust the moisture content, and finally, it was packaged under sterile conditions to make weakly acidic wet toilet paper.

[0014] Furthermore, the preparation of the plant-derived weakly acidic regulating composite in step S1 specifically includes the following steps:

[0015] S1.1: Select cranberries, remove the moldy fruits, then soak them in a 0.1% sodium hypochlorite solution for 5 - 10 min for sterilization, rinse with pure water until neutral, and then crush them with a twin-screw extrusion crusher at 200 - 300 rpm to obtain a fruit pulp with a particle size of 2 - 3 mm;

[0016] S1.2: The fruit pulp was mixed with 95% ethanol in a volume ratio of 1:3 - 4 and then added to an extraction kettle. The pressure of the extraction kettle was 25 - 28 MPa, the temperature was 40 - 45 °C, the CO2 flow rate was 30 L / min, and extraction was carried out for 2 - 3 h. The extract was collected and filtered through a 50 kDa ceramic membrane to obtain a refined cranberry extract;

[0017] S1.3: Lactobacillus plantarum and Lactobacillus rhamnosus were compounded at a ratio of 3 - 4:1 to obtain a mixed strain. The mixed strain was inoculated into MRS liquid medium with an inoculation amount of 5 wt%, anaerobically cultured at 37 °C for 18 - 20 h, and then fermented at a controlled temperature of 32 °C for 48 - 50 h. During this period, 5% NaOH was added dropwise to maintain the pH at 4.5 - 4.8. After fermentation was completed, inactivation was carried out, and centrifugation and filtration were performed to obtain a lactic acid bacteria fermentation broth;

[0018] S1.4: The refined cranberry extract and the lactic acid bacteria fermentation broth were mixed at a volume ratio of 3 - 4:7, and 0.1 M sodium citrate buffer solution was added dropwise. The pH of the mixed solution was adjusted to 5.0 - 5.2, and then it was stirred at 200 - 300 rpm in a water bath at 45 - 50 °C for 30 - 40 min. Finally, 0.05 wt% xanthan gum was added to obtain a plant-derived weakly acidic regulating composite.

[0019] Further, the preparation of the antibacterial microcapsule complex in step S2 specifically includes the following steps:

[0020] S2.1: Add 2 - 3 parts by weight of nano - zinc oxide into 100 - 120 parts by weight of an ethanol - aqueous solution with a concentration of 50%, ultrasonically disperse for 20 - 30 min, then add 2 - 3 parts by weight of 3 - aminopropyltriethoxysilane, then adjust the pH to 9 - 10, stir at room temperature for 2 - 3 h, then wash carefully and dry to obtain surface - modified nano - zinc oxide;

[0021] S2.2: Add 0.5 - 1 part by weight of ε - polylysine into 100 - 120 parts by weight of deionized water, then adjust the pH to 6.1 - 6.3, then add 0.3 - 0.5 part by weight of surface - modified nano - zinc oxide, and stir - mix at 200 - 300 r / min for 2 - 3 h to obtain an ε - polylysine / nano - zinc oxide complex;

[0022] S2.3: Add 2 - 3 parts by weight of chitosan into 100 - 120 parts by weight of an acetic acid buffer solution with a pH of 4.5, stir - mix for 20 - 30 min, then add 0.02 - 0.03 part by weight of carboxyl - terminal - activated targeting antibacterial peptide, then add 1 - 2 parts by weight of glutaraldehyde, react at room temperature for 2 - 3 h, then adjust the pH to neutral with a NaOH solution, and obtain targeted chitosan after dialysis;

[0023] S2.4: Add 2 - 3 parts by weight of sodium alginate into 100 - 120 parts by weight of deionized water, then add 3 - 5 parts by weight of the ε - polylysine / nano - zinc oxide complex, stir - mix to obtain an aqueous solution. Add 0.5 - 0.8 part by weight of targeted chitosan into an acetic acid buffer solution with a pH of 5, then add it into 200 - 230 parts by weight of liquid paraffin to obtain an oil - phase solution. Mix the oil - phase solution and the aqueous solution according to a weight - part ratio of 2 - 3:1, homogenize and emulsify to form a W / O emulsion, then add 500 - 520 parts by weight of a CaCl2 solution, react for 30 - 50 min, then wash, centrifuge, and dry to obtain the antibacterial microcapsule complex.

[0024] Further, the preparation of the weakly acidic wet toilet paper in step S3 specifically includes the following steps:

[0025] S3.1: Add 3 - 5 parts by weight of glycerol glucoside, 0.1 - 0.3 part by weight of xanthan gum, 0.05 - 0.08 part by weight of octanoyl hydroxamic acid into 100 parts by weight of ultrapure water at 40 - 50 °C, magnetically stir at 500 - 800 rpm for 20 - 30 min, then add 8 - 10 parts by weight of the antibacterial microcapsule complex, and stir at 200 - 300 rpm for 5 - 10 min to obtain a microcapsule suspension;

[0026] S3.2: Immerse the non-woven fabric substrate in the microcapsule suspension, perform vacuum adsorption at -0.08 MPa for 20 - 30 min, then conduct hot air drying at 60 - 65 °C for 1 - 2 h. After that, immerse it in the treatment liquid and react at 70 - 75 °C for 1 - 2 h. Then rinse and dry to obtain a double-modified non-woven fabric substrate;

[0027] S3.3: Add 3 - 5 parts by weight of glucosylglycerol and 0.05 - 0.08 parts by weight of octanohydroxamic acid to 100 parts by weight of ultrapure water at 40 - 50 °C, stir magnetically at 500 - 800 rpm for 20 - 30 min, then add 8 - 10 parts by weight of the plant-derived weak acid regulating complex and stir at 200 - 300 rpm for 5 - 10 min to obtain a wet system solution;

[0028] S3.4: Immerse the double-modified non-woven fabric substrate in the wet system solution, adjust the final moisture content to 160 - 180%, and seal and package it with a film of matte transparent OPP, aluminized VMPET, and shiny transparent PE under sterile conditions to make a weakly acidic wet toilet paper.

[0029] Furthermore, in step S1.1, the cranberries are high-acidity cranberries with a total acid content of ≥2.5%.

[0030] Furthermore, the liquid paraffin in step S2.4 is liquid paraffin containing 2 wt% Span-80.

[0031] Furthermore, in step S3.2, the treatment liquid is 2 - 3 wt% methacryloyloxyethyltrimethylammonium chloride and 0.5 - 0.8 wt% ammonium persulfate, with the balance being deionized water.

[0032] A weakly acidic wet toilet paper for women, which is prepared by the preparation process of a weakly acidic wet toilet paper for women described in any one of the above.

[0033] Compared with the prior art, the present invention has at least the following beneficial effects:

[0034] 1. The present invention combines cranberry refined extract and lactic acid bacteria fermentation broth, and through pH adjustment, finally stabilizes the pH of the wet toilet paper close to the normal pH range of the female private parts, which can avoid the destruction of the acid-base barrier of the private parts by alkaline components, inhibit the overgrowth of harmful bacteria such as Staphylococcus aureus and Escherichia coli, and at the same time create a suitable living environment for beneficial bacteria such as Lactobacillus, reducing the risk of gynecological inflammation. The proanthocyanidins and polyphenolic substances in the cranberry refined extract can inhibit the adhesion of pathogenic bacteria to the mucous membranes of the urogenital tract, reducing the infection probability. Antioxidant components such as vitamin C and flavonoids can relieve the redness and itching of the private part skin caused by friction and secretion stimulation, and enhance the skin barrier function. The lactic acid bacteria fermentation broth contains lactic acid, hydrogen peroxide and bacteriocin, which directly inhibit the growth of harmful bacteria through acidification of the environment and competitive exclusion, and bidirectionally regulate the microecological balance. Amino acids and short-chain fatty acids have moisturizing and soothing effects, reducing the mechanical irritation during the use of wet toilet paper.

[0035] 2. In the present invention, the sodium alginate-chitosan microcapsule forms a stable capsule wall through ionic cross-linking, gradually releases antibacterial components when wiping and contacting water, prolongs the effective antibacterial time, and reduces the interference with the skin microecology caused by frequent use. Nano-zinc oxide is combined with ε-polylysine to cover Gram-positive / negative bacteria and fungi. The targeted antibacterial peptide recognizes the pathogen surface receptor through the carboxyl terminal, accurately kills pathogenic bacteria, and reduces the impact of broad-spectrum antibacterial agents on beneficial bacteria. Chitosan adsorbs on the bacterial surface through positive charges, increases the cell membrane permeability, and cooperates with the targeted antibacterial peptide to destroy the polysaccharide backbone of the biofilm, blocking the escape mechanism of drug-resistant bacteria. The silane coating reduces the free release of Zn2+ and reduces the irritation to sensitive mucous membranes caused by direct contact. The antibacterial components released by the microcapsule and cranberry proanthocyanidins and lactic acid bacteria metabolites form a "prompt antibacterial-long-term regulation" dual path, reducing the recurrence rate of vaginitis. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] The drawings incorporated herein and constituting a part of the specification illustrate embodiments of the present invention, and together with the specification are further used to explain the principles of the present invention and enable those skilled in the relevant art to implement and use the present invention.

[0037] Figure 1 It is a process flow chart of the preparation of a female-specific weakly acidic wet toilet paper adopted in the embodiments of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0038] The following describes in detail the preparation process of a female-specific weakly acidic wet toilet paper provided by the present invention in conjunction with the drawings and specific embodiments. At the same time, it is noted here that in order to make the embodiments more detailed, the following embodiments are the best and preferred embodiments. For some well-known technologies, those skilled in the art can also adopt other alternative methods for implementation; moreover, the drawing part is only for more specifically describing the embodiments and is not intended to specifically limit the present invention.

[0039] Example 1

[0040] A preparation process of female-specific weakly acidic wet toilet paper is as Figure 1 shown, and it includes the following steps:

[0041] S1: Preparation of plant-derived weakly acidic regulating complex

[0042] S1.1: Select high-acidity cranberries with a total acid content ≥ 2.5%, remove mildewed fruits, then soak them in a 0.1% sodium hypochlorite solution for 5 min for sterilization, rinse with pure water until neutral, and then crush them with a twin-screw extrusion crusher at 200 rpm to obtain a fruit pulp with a particle size of 2 - 3 mm;

[0043] S1.2: Mix the fruit pulp with ethanol with a concentration of 95% according to a volume ratio of 1:3, add it to the extraction kettle, with the pressure of the extraction kettle being 25 MPa, the temperature being 40 °C, the CO2 flow rate being 30 L / min, extract for 2 h, collect the extract, and filter the extract through a 50 kDa ceramic membrane to obtain a refined cranberry extract;

[0044] S1.3: Compound Lactobacillus plantarum and Lactobacillus rhamnosus in a ratio of 3:1 to obtain a mixed strain. Inoculate the mixed strain into MRS liquid medium with an inoculation amount of 5 wt%, anaerobically culture at 37 °C for 18 h, then carry out temperature-controlled fermentation at 32 °C for 48 h, during which add 5% NaOH dropwise to maintain pH 4.5. After fermentation is completed, inactivate it, carry out centrifugal separation, and filter to obtain a lactic acid bacteria fermentation broth;

[0045] S1.4: Mix the refined cranberry extract and the lactic acid bacteria fermentation broth according to a volume ratio of 3:7, gradually add 0.1 M sodium citrate buffer solution, adjust the pH of the mixed solution to 5, then stir at 200 rpm in a 45 °C water bath for 30 min, and finally add 0.05 wt% xanthan gum to obtain a plant-derived weakly acidic regulating complex;

[0046] S2: Preparation of antibacterial microcapsule complex

[0047] S2.1: Add 2 - 3 parts by weight of nano-zinc oxide to 100 parts by weight of an ethanol aqueous solution with a concentration of 50%, ultrasonically disperse for 20 min, then add 2 parts by weight of 3-aminopropyltriethoxysilane, then adjust the pH to 9, stir at room temperature for 2 - 3 h, then wash carefully and dry to obtain surface-modified nano-zinc oxide;

[0048] S2.2: Add 0.5 part by weight of ε-polylysine to 100 parts by weight of deionized water, then adjust the pH to 6.1, then add 0.3 part by weight of surface-modified nano-zinc oxide, and stir and mix at 200 r / min for 2 h to obtain an ε-polylysine / nano-zinc oxide complex;

[0049] S2.3: Add 2 parts by weight of chitosan into 100 parts by weight of acetic acid buffer solution with a pH of 4.5, stir and mix for 20 min, then add 0.02 parts by weight of carboxyl-terminally activated targeted antibacterial peptide, and then add 1 part by weight of glutaraldehyde. React at room temperature for 2 h, and then adjust the pH to neutral with NaOH solution. After dialysis, targeted chitosan is obtained.

[0050] S2.4: Add 2 parts by weight of sodium alginate into 100 parts by weight of deionized water, and then add 3 parts by weight of ε-polylysine / nano-zinc oxide complex, stir and mix to obtain an aqueous solution. Add 0.5 parts by weight of targeted chitosan into acetic acid buffer solution with a pH of 5, and then add it into 200 parts by weight of liquid paraffin containing 2 wt% Span-80 to obtain an oil phase solution. Mix the oil phase solution and the aqueous solution according to a weight ratio of 2:1, and then homogenize and emulsify to form a W / O emulsion. Then add 500 parts by weight of CaCl2 solution, react for 30 min, and then wash, centrifuge, and dry to obtain an antibacterial microcapsule complex.

[0051] S3: Preparation of weakly acidic wet toilet paper

[0052] S3.1: Add 3 parts by weight of glucosylglycerol, 0.1 part by weight of xanthan gum, and 0.05 part by weight of octanoyl hydroxamic acid into 100 parts by weight of ultrapure water at 40 °C, stir magnetically at 500 rpm for 20 min, then add 8 parts by weight of antibacterial microcapsule complex, and stir at 200 rpm for 5 min to obtain a microcapsule suspension.

[0053] S3.2: Immerse the non-woven fabric substrate in the microcapsule suspension, adsorb it under -0.08 MPa vacuum for 20 min, then dry it in hot air at 60 °C for 1 h, then immerse it in the treatment solution again, react at 70 °C for 1 h, and then rinse and dry to obtain a double-modified non-woven fabric substrate.

[0054] S3.3: Add 3 parts by weight of glucosylglycerol and 0.05 part by weight of octanoyl hydroxamic acid into 100 parts by weight of ultrapure water at 40 °C, stir magnetically at 500 rpm for 20 min, then add 8 parts by weight of plant-derived weak acid regulating complex, and stir at 200 rpm for 5 min to obtain a wetting system solution.

[0055] S3.4: Immerse the double-modified non-woven fabric substrate in the wetting system solution, adjust the final moisture content to 160%, and seal and package it with a film of matte transparent OPP, aluminized VMPET, and bright transparent PE under sterile conditions to make a weakly acidic wet toilet paper.

[0056] The treatment solution is 2 wt% methacryloyloxyethyl trimethyl ammonium chloride and 0.5 wt% ammonium persulfate, and the balance is deionized water.

[0057] Example 2

[0058] A preparation process of a weakly acidic wet toilet paper for women only, as Figure 1 shown, includes the following steps:

[0059] S1: Preparation of plant-derived weakly acidic regulating complex

[0060] S1.1: Select high-acidity cranberries with a total acid content ≥ 2.5%, remove moldy fruits, then soak them in a 0.1% sodium hypochlorite solution for 5 min for sterilization, rinse with pure water until neutral, and then crush them with a twin-screw extrusion crusher at 200 rpm to obtain a fruit pulp with a particle size of 2 - 3 mm;

[0061] S1.2: Mix the fruit pulp with ethanol with a concentration of 95% in a volume ratio of 1:4, add it to the extraction kettle, the pressure of the extraction kettle is 25 MPa, the temperature is 40 °C, the CO2 flow rate is 30 L / min, extract for 2 h, collect the extract, and filter the extract through a 50 kDa ceramic membrane to obtain a refined cranberry extract;

[0062] S1.3: Compound Lactobacillus plantarum and Lactobacillus rhamnosus in a ratio of 4:1 to obtain a mixed strain, inoculate the mixed strain into MRS liquid medium, the inoculation amount is 5 wt%, anaerobically culture at 37 °C for 18 h, and then carry out temperature-controlled fermentation at 32 °C for 48 h. During this period, add 5% NaOH dropwise to maintain pH 4.8. After fermentation is completed, inactivate, centrifuge and separate, and filter to obtain a lactic acid bacteria fermentation broth;

[0063] S1.4: Mix the refined cranberry extract and the lactic acid bacteria fermentation broth in a volume ratio of 4:7, gradually add 0.1 M sodium citrate buffer solution, adjust the pH of the mixed solution to 5.2, then stir at 200 rpm in a 45 °C water bath for 30 min, and finally add 0.05 wt% xanthan gum to obtain a plant-derived weakly acidic regulating complex;

[0064] S2: Preparation of antibacterial microcapsule complex

[0065] S2.1: Add 3 parts by weight of nano-zinc oxide to 120 parts by weight of an ethanol aqueous solution with a concentration of 50%, ultrasonically disperse for 20 min, then add 3 parts by weight of 3-aminopropyltriethoxysilane, then adjust the pH to 10, stir at room temperature for 2 h, and then carefully wash and dry to obtain surface-modified nano-zinc oxide;

[0066] S2.2: Add 1 part by weight of ε-polylysine to 120 parts by weight of deionized water, then adjust the pH to 6.3, then add 0.5 part by weight of surface-modified nano-zinc oxide, and stir and mix at 200 r / min for 2 h to obtain an ε-polylysine / nano-zinc oxide complex;

[0067] S2.3: Add 3 parts by weight of chitosan into 120 parts by weight of acetic acid buffer solution with a pH of 4.5, stir and mix for 20 min, then add 0.03 part by weight of carboxyl-terminal activated targeting antibacterial peptide, and then add 2 parts by weight of glutaraldehyde. React at room temperature for 2 h, and then adjust the pH to neutral with NaOH solution. After dialysis, the targeted chitosan is obtained;

[0068] S2.4: Add 3 parts by weight of sodium alginate into 120 parts by weight of deionized water, and then add 5 parts by weight of ε-polylysine / nano-zinc oxide complex, stir and mix to obtain an aqueous solution. Add 0.8 part by weight of targeted chitosan into acetic acid buffer solution with a pH of 5, and then add it into 230 parts by weight of liquid paraffin containing 2 wt% Span-80 to obtain an oil-phase solution. Mix the oil-phase solution and the aqueous solution according to the weight ratio of 3:1, and then homogenize and emulsify to form a W / O emulsion. Then add 520 parts by weight of CaCl2 solution, react for 30 min, and then wash, centrifuge and dry to obtain the antibacterial microcapsule complex;

[0069] S3: Preparation of weakly acidic wet toilet paper

[0070] S3.1: Add 5 parts by weight of glucosylglycerol, 0.3 part by weight of xanthan gum, and 0.08 part by weight of octanoyl hydroxamic acid into 100 parts by weight of ultrapure water at 40 °C, stir magnetically at 500 rpm for 20 min, then add 10 parts by weight of antibacterial microcapsule complex, and stir at 200 rpm for 5 min to obtain a microcapsule suspension;

[0071] S3.2: Immerse the non-woven fabric substrate in the microcapsule suspension, adsorb it under -0.08 MPa vacuum for 20 min, then dry it with hot air at 60 °C for 1 h, then immerse it in the treatment solution again, react at 70 °C for 1 h, and then rinse and dry to obtain a double-modified non-woven fabric substrate;

[0072] S3.3: Add 5 parts by weight of glucosylglycerol and 0.08 part by weight of octanoyl hydroxamic acid into 100 parts by weight of ultrapure water at 40 °C, stir magnetically at 500 rpm for 20 min, then add 10 parts by weight of plant-derived weak acid regulating complex, and stir at 200 rpm for 5 min to obtain a wetting system solution;

[0073] S3.4: Immerse the double-modified non-woven fabric substrate in the wetting system solution, adjust the final moisture content to 180%, and seal and package it with a film of matte transparent OPP, aluminized VMPET, and bright transparent PE under sterile conditions to make the weakly acidic wet toilet paper;

[0074] The treatment solution is 3 wt% methacryloyloxyethyl trimethyl ammonium chloride and 0.8 wt% ammonium persulfate, and the balance is deionized water.

[0075] Example 3

[0076] A preparation process of a weakly acidic wet toilet paper for women, as Figure 1 shown, includes the following steps:

[0077] S1: Preparation of a plant-derived weakly acidic regulating complex

[0078] S1.1: Select high-acidity cranberries with a total acid content ≥ 2.5%, remove moldy fruits, then soak them in a 0.1% sodium hypochlorite solution for 10 min for sterilization, rinse with pure water until neutral, and then crush them with a twin-screw extrusion crusher at 300 rpm to obtain a fruit pulp with a particle size of 2 - 3 mm;

[0079] S1.2: Mix the fruit pulp with ethanol with a concentration of 95% in a volume ratio of 1:3, add it to an extraction kettle, with the pressure of the extraction kettle being 28 MPa, the temperature being 45 °C, the CO2 flow rate being 30 L / min, extract for 3 h, collect the extract, and filter the extract through a 50 kDa ceramic membrane to obtain a refined cranberry extract;

[0080] S1.3: Compound Lactobacillus plantarum and Lactobacillus rhamnosus in a ratio of 3:1 to obtain a mixed strain. Inoculate the mixed strain into an MRS liquid medium with an inoculation amount of 5 wt%, anaerobically culture at 37 °C for 20 h, and then carry out temperature-controlled fermentation at 32 °C for 50 h. During this period, add 5% NaOH dropwise to maintain the pH at 4.5. After fermentation is completed, inactivate, centrifuge and separate, and filter to obtain a lactic acid bacteria fermentation broth;

[0081] S1.4: Mix the refined cranberry extract and the lactic acid bacteria fermentation broth in a volume ratio of 3:7, gradually add 0.1 M sodium citrate buffer solution, adjust the pH of the mixed solution to 5, then stir at 300 rpm in a 50 °C water bath for 40 min, and finally add 0.05 wt% xanthan gum to obtain a plant-derived weakly acidic regulating complex;

[0082] S2: Preparation of an antibacterial microcapsule complex

[0083] S2.1: Add 2 parts by weight of nano-zinc oxide to 100 parts by weight of an ethanol aqueous solution with a concentration of 50%, ultrasonically disperse for 30 min, then add 2 parts by weight of 3-aminopropyltriethoxysilane, then adjust the pH to 9, stir at room temperature for 3 h, and then wash carefully and dry to obtain surface-modified nano-zinc oxide;

[0084] S2.2: Add 0.5 part by weight of ε-polylysine to 100 parts by weight of deionized water, then adjust the pH to 6.1, then add 0.3 part by weight of surface-modified nano-zinc oxide, and stir and mix at 300 r / min for 3 h to obtain an ε-polylysine / nano-zinc oxide complex;

[0085] S2.3: Add 2 parts by weight of chitosan to 100 parts by weight of acetic acid buffer solution with a pH of 4.5, stir and mix for 30 min, then add 0.02 parts by weight of carboxyl-terminal activated targeted antibacterial peptide, and then add 1 part by weight of glutaraldehyde. React at room temperature for 3 h, and then adjust the pH to neutral with NaOH solution. After dialysis, targeted chitosan is obtained;

[0086] S2.4: Add 2 parts by weight of sodium alginate to 100 parts by weight of deionized water, and then add 3 parts by weight of ε-polylysine / nano-zinc oxide composite, stir and mix to obtain an aqueous solution. Add 0.5 parts by weight of targeted chitosan to acetic acid buffer solution with a pH of 5, and then add it to 200 parts by weight of liquid paraffin containing 2 wt% Span-80 to obtain an oil phase solution. Mix the oil phase solution and the aqueous solution according to the weight ratio of 2:1, and then homogenize and emulsify to form a W / O emulsion. Then add 500 parts by weight of CaCl2 solution, react for 50 min, and then wash, centrifuge, and dry to obtain an antibacterial microcapsule composite;

[0087] S3: Preparation of weakly acidic wet toilet paper

[0088] S3.1: Add 3 parts by weight of glucosylglycerol, 0.1 part by weight of xanthan gum, and 0.05 part by weight of octanoyl hydroxamic acid to 100 parts by weight of ultrapure water at 50 °C, stir magnetically at 800 rpm for 30 min, then add 8 parts by weight of antibacterial microcapsule composite, and stir at 300 rpm for 10 min to obtain a microcapsule suspension;

[0089] S3.2: Immerse the non-woven fabric substrate in the microcapsule suspension, adsorb it under -0.08 MPa vacuum for 30 min, then dry it with hot air at 65 °C for 2 h, then immerse it in the treatment solution again, react at 75 °C for 2 h, and then rinse and dry to obtain a double-modified non-woven fabric substrate;

[0090] S3.3: Add 3 parts by weight of glucosylglycerol and 0.05 part by weight of octanoyl hydroxamic acid to 100 parts by weight of ultrapure water at 50 °C, stir magnetically at 800 rpm for 30 min, then add 8 parts by weight of plant-derived weakly acidic regulating composite, and stir at 300 rpm for 10 min to obtain a wetting system solution;

[0091] S3.4: Immerse the double-modified non-woven fabric substrate in the wetting system solution, adjust the final moisture content to 160%, and seal and package it with a film of matte transparent OPP, aluminized VMPET, and bright transparent PE under sterile conditions to make weakly acidic wet toilet paper;

[0092] The treatment solution is 2 wt% methacryloyloxyethyl trimethyl ammonium chloride and 0.5 wt% ammonium persulfate, and the balance is deionized water.

[0093] Comparative Example 1

[0094] Compared with Example 1, the difference in Comparative Example 1 is that in Comparative Example 1, the plant-derived weak acidic regulating complex in Step S1 and Step S3.3 is removed, and the remaining steps remain unchanged to prepare the weakly acidic wet toilet paper, denoted as Comparative Example 1.

[0095] Comparative Example 2

[0096] Compared with Example 1, the difference in Comparative Example 2 is that in Comparative Example 2, Steps S2 and S3.1 are removed, and the remaining steps remain unchanged to prepare the weakly acidic wet toilet paper, denoted as Comparative Example 2.

[0097] The irritation degree, pH value, and bactericidal rate of the weakly acidic wet toilet paper prepared in Examples 1-3 were detected, and the test results are shown in Table 1 for reference.

[0098] Table 1. Performance Test of Examples 1-3

[0099] Stimulation degree IS value pH value Sterilization rate (%) Example 1 1.12 5 99.9 Example 2 1.13 5.2 99.9 Example 3 1.13 5 99.8

[0100] It can be seen from the data in Table 1 that the IS value of the irritation degree of the weakly acidic wet toilet paper prepared by the present invention is relatively low, thus reducing the redness, swelling, itching of the skin of the female private parts, being beneficial to health, and the pH value is close to the normal pH range of the female private parts, which can avoid the destruction of the acid-base barrier of the private parts by alkaline components, and at the same time has excellent antibacterial effects.

[0101] The irritation degree of Comparative Example 1 was detected, and the test results are shown in Table 2 for reference.

[0102] Table 2. Test Results of the Irritation Degree of Comparative Example 1

[0103] Stimulation degree IS value Example 1 1.12 Comparative example 1 2.03

[0104] It can be seen from the data in Table 2 that the absence of the plant-derived weak acidic regulating complex will significantly weaken the inhibitory degree of irritation, resulting in an increase in the irritation degree. Therefore, adding the plant-derived weak acidic regulating complex can relieve the redness and itching of the private part skin caused by friction and secretion irritation, and enhance the skin barrier function.

[0105] The bactericidal rates of Comparative Examples 1-2 were detected, and the test results are shown in Table 3 for reference.

[0106] Table 3. Test Results of the Bactericidal Rates of Comparative Examples 1-2

[0107] Sterilization rate (%) Example 1 99.9 Comparative example 1 93.2 Comparative example 2 73.2

[0108] It can be seen from the data in Table 3 that adding both the plant-derived weak acidic regulating complex and the antibacterial microcapsule complex effectively improves the antibacterial effect of the weakly acidic wet toilet paper.

[0109] The above embodiments are only illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Any person familiar with this technology can modify or change the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or changes made by those with ordinary knowledge in the technical field without departing from the spirit and technical idea disclosed by the present invention should still be covered by the claims of the present invention.

Claims

1. A preparation process for weakly acidic wet toilet paper for women, characterized in that, It includes the following steps: S1: Preparation of plant-derived weakly acidic regulating complex Compound the refined cranberry extract and lactic acid bacteria fermentation broth to prepare a plant-derived weakly acidic regulating complex; S2: Preparation of antibacterial microcapsule complex Surface-modify nano-zinc oxide with 3-aminopropyltriethoxysilane, then mix it with ε-polylysine to prepare an ε-polylysine / nano-zinc oxide complex. Then, use sodium alginate and the ε-polylysine / nano-zinc oxide complex as the aqueous phase, and targeted chitosan and liquid paraffin as the oil phase to prepare an antibacterial microcapsule complex; S3: Preparation of weakly acidic wet toilet paper Prepare a microcapsule suspension with glucosylglycerol, xanthan gum, octanohydroxamic acid and an antibacterial microcapsule complex, then immerse it in a treatment solution to obtain a double-modified non-woven fabric substrate. Mix glucosylglycerol, octanohydroxamic acid and a plant-derived weakly acidic regulating complex to prepare a wetting system solution. Then immerse the double-modified non-woven fabric substrate in the wetting system solution, adjust the moisture content, and finally package it under sterile conditions to make weakly acidic wet toilet paper.

2. The preparation process of a weakly acidic wet toilet paper for women according to claim 1, characterized in that, The preparation of the plant-derived weakly acidic regulating complex in step S1 specifically includes the following steps: S1.1: Select cranberries, remove the moldy fruits, then soak them in a 0.1% sodium hypochlorite solution for 5 - 10 min for sterilization, rinse with pure water until neutral, and then crush them with a twin-screw extrusion crusher at 200 - 300 rpm to obtain a fruit pulp with a particle size of 2 - 3 mm; S1.2: Mix the fruit pulp with 95% ethanol in a volume ratio of 1:3 - 4 and add it to an extraction kettle. The pressure of the extraction kettle is 25 - 28 MPa, the temperature is 40 - 45 °C, the CO2 flow rate is 30 L / min, and extract for 2 - 3 h. Collect the extract and filter it through a 50 kDa ceramic membrane to obtain a refined cranberry extract; S1.3: Compound Lactobacillus plantarum and Lactobacillus rhamnosus at a ratio of 3 - 4:1 to obtain a mixed strain. Inoculate the mixed strain into MRS liquid medium at an inoculation amount of 5 wt%, anaerobically culture at 37 °C for 18 - 20 h, and then carry out temperature-controlled fermentation at 32 °C for 48 - 50 h. During this period, add 5% NaOH dropwise to maintain the pH at 4.5 - 4.

8. After fermentation is completed, inactivate it, centrifuge and filter to obtain a lactic acid bacteria fermentation broth; S1.4: Mix the refined cranberry extract and the lactic acid bacteria fermentation broth in a volume ratio of 3 - 4:7, gradually add 0.1 M sodium citrate buffer solution, adjust the pH of the mixed solution to 5.0 - 5.2, then stir at 200 - 300 rpm in a 45 - 50 °C water bath for 30 - 40 min, and finally add 0.05 wt% xanthan gum to obtain a plant-derived weakly acidic regulating complex.

3. The preparation process of a weakly acidic wet toilet paper for women according to claim 2, characterized in that, The preparation of the antibacterial microcapsule complex in step S2 specifically includes the following steps: S2.1: Add 2 - 3 parts by weight of nano-zinc oxide to 100 - 120 parts by weight of a 50% ethanol aqueous solution, ultrasonically disperse for 20 - 30 min, then add 2 - 3 parts by weight of 3-aminopropyltriethoxysilane, then adjust the pH to 9 - 10, stir at room temperature for 2 - 3 h, then carefully wash and dry to obtain surface-modified nano-zinc oxide; S2.2: Add 0.5 - 1 part by weight of ε - polylysine into 100 - 120 parts by weight of deionized water, then adjust the pH to 6.1 - 6.3, and then add 0.3 - 0.5 part by weight of surface - modified nano - zinc oxide. Stir and mix at 200 - 300 r / min for 2 - 3 h to obtain the ε - polylysine / nano - zinc oxide complex; S2.3: Add 2 - 3 parts by weight of chitosan into 100 - 120 parts by weight of acetic acid buffer solution with a pH of 4.5, stir and mix for 20 - 30 min, then add 0.02 - 0.03 part by weight of carboxyl - terminal activated targeting antibacterial peptide, and then add 1 - 2 parts by weight of glutaraldehyde. React at room temperature for 2 - 3 h, and then adjust the pH to neutral with NaOH solution. After dialysis, obtain the targeting chitosan; S2.4: Add 2 - 3 parts by weight of sodium alginate into 100 - 120 parts by weight of deionized water, and then add 3 - 5 parts by weight of the ε - polylysine / nano - zinc oxide complex, stir and mix to obtain an aqueous solution. Add 0.5 - 0.8 part by weight of targeting chitosan into acetic acid buffer solution with a pH of 5, and then add it into 200 - 230 parts by weight of liquid paraffin to obtain an oil - phase solution. Mix the oil - phase solution and the aqueous solution according to the weight - part ratio of 2 - 3:1, and then homogenize and emulsify to form a W / O emulsion. Then add 500 - 520 parts by weight of CaCl₂ solution, react for 30 - 50 min, and then wash, centrifuge, and dry to obtain the antibacterial micro - capsule complex.

4. The preparation process of a female-specific weakly acidic wet toilet paper according to claim 3, characterized in that, Step S3 Preparation of weakly acidic wet toilet paper, specifically including the following steps: S3.1: Add 3 - 5 parts by weight of glucosylglycerol, 0.1 - 0.3 part by weight of xanthan gum, 0.05 - 0.08 part by weight of octanoyl hydroxamic acid into 100 parts by weight of ultrapure water at 40 - 50 °C, stir magnetically at 500 - 800 rpm for 20 - 30 min, then add 8 - 10 parts by weight of the antibacterial micro - capsule complex, and stir at 200 - 300 rpm for 5 - 10 min to obtain a micro - capsule suspension; S3.2: Immerse the non - woven fabric substrate into the micro - capsule suspension, adsorb it under - 0.08 MPa vacuum for 20 - 30 min, then dry it by hot air at 60 - 65 °C for 1 - 2 h, then immerse it in the treatment solution, react at 70 - 75 °C for 1 - 2 h, and then rinse and dry to obtain the double - modified non - woven fabric substrate; S3.3: Add 3 - 5 parts by weight of glucosylglycerol, 0.05 - 0.08 part by weight of octanoyl hydroxamic acid into 100 parts by weight of ultrapure water at 40 - 50 °C, stir magnetically at 500 - 800 rpm for 20 - 30 min, then add 8 - 10 parts by weight of the plant - derived weak - acidity regulating complex, and stir at 200 - 300 rpm for 5 - 10 min to obtain a wetting system solution; S3.4: Immerse the double - modified non - woven fabric substrate in the wetting system solution, adjust the final moisture content to 160 - 180%, and seal and package it with a film of matte transparent OPP, aluminized VMPET, and bright transparent PE under sterile conditions to make the weakly acidic wet toilet paper.

5. The preparation process of a weakly acidic wet toilet paper for women according to claim 2, characterized in that, In step S1.1, the cranberries are high - acidity cranberries with a total acid content ≥2.5%.

6. The preparation process of a female-specific weakly acidic wet toilet paper according to claim 3, characterized in that, The liquid paraffin in step S2.4 is liquid paraffin containing 2 wt% Span-80.

7. The preparation process of a female-specific weakly acidic wet toilet paper according to claim 4, characterized in that, In step S3.2, the treatment liquid is 2-3 wt% methacryloyloxyethyl trimethyl ammonium chloride and 0.5-0.8 wt% ammonium persulfate, and the balance is deionized water.

8. A weakly acidic wet toilet paper for women, characterized in that, It is prepared by the preparation process of a weakly acidic wet toilet paper for women according to any one of claims 1-7.

Images