1883 results about "Lactic acid bacterium" patented technology

The invention relates to the field of equipment operation and maintenance management, and discloses an operation and maintenance management method and system for lactic acid bacteria fermentation equipment, and the method comprises the following steps: S1, monitoring the temperature, humidity, pressure and rotating speed parameters of the equipment in real time through a sensor and an Internet of Things technology, so as to obtain monitoring data; s2, preprocessing the monitoring data, and transmitting the preprocessed monitoring data to an operation and maintenance management platform for storage; s3, each equipment parameter of the monitoring data is compared with a preset fault threshold value, when any one equipment parameter reaches or exceeds the preset fault threshold value, it is judged that the current equipment operates abnormally, and otherwise, it is judged that the current equipment operates normally; s4, judging that the abnormally running equipment can automatically trigger an early warning mechanism to carry out early warning; and S5, carrying out secondary recheck on the equipment which triggers the early warning, obtaining a comprehensive operation evaluation index according to monitoring data processing, and judging whether the current equipment has a fault risk or not according to the comprehensive operation evaluation index.

The invention relates to the technical field of food processing, in particular to oat fermented milk and a preparation method thereof, and the preparation method comprises the following steps: respectively preparing bacterial suspensions of TZ3-1-2, TG8-1-9 and QF-1-1; adding the TZ3-1-2 bacterial suspension and the TG8-1-9 bacterial suspension into the oat juice, and carrying out lactic acid bacteria fermentation to obtain a lactic acid bacteria fermentation product; adding the QF-1-1 bacterial suspension into the lactic acid bacteria fermentation product for yeast fermentation to obtain a yeast fermentation product; and carrying out after-ripening catalysis on the yeast fermentation product to obtain the oat fermented milk. The lactic acid bacteria TZ3-1-2 are pediococcus pentosaceus, and the preservation number of the lactic acid bacteria TZ3-1-2 is CGMCC (China General Microbiological Culture Collection Center) No.32259; the lactic acid bacteria TG8-1-9 are lactobacillus plantarum, and the preservation number of the lactic acid bacteria TG8-1-9 is CGMCC (China General Microbiological Culture Collection Center) No.32258. The lactic acid bacteria TG8-1-9 are lactobacillus plantarum. According to the method, mixed fermentation and two-stage sequential fermentation technologies are utilized, the fermentation time is shortened, meanwhile, the defects of single fermentation are effectively overcome, and finally the oat fermented milk which is excellent in taste and good in tissue state in all aspects is obtained.

The invention relates to the technical field of microorganisms, in particular to application of lactobacillus rhamnosus KF7 in improving intestinal barrier injury and regulating intestinal flora and functional food containing the lactobacillus rhamnosus KF7. The invention provides application of lactobacillus rhamnosus KF7 in preparation of a product for improving intestinal barrier injury and / or regulating intestinal flora. The preservation number of the lactobacillus rhamnosus KF7 is CGMCC (China General Microbiological Culture Collection Center) NO.6430. The invention further provides application of the lactobacillus rhamnosus KF7 in preparation of a product for improving intestinal barrier injury and / or regulating intestinal flora. The invention discloses the novel application of the caseus rhamnosus KF7 in the aspects of improving the normal intestinal epithelial barrier function, protecting the normal intestinal epithelial barrier from lipopolysaccharide induced barrier injury, recovering animal intestinal barrier injury caused by rhythm disorder, regulating intestinal flora disorder and improving growth, development and metabolic states for the first time. The method can be used for producing functional food products such as fermented milk, lactic acid bacteria beverages, probiotic powder, viable bacteria water, milk powder and cheese.

The invention relates to the technical field of feed additives, and discloses a feed additive and application thereof in yak breeding. The feed additive comprises the following raw materials in parts by mass: 10-20 parts of mixed bean flour, 5-10 parts of a traditional Chinese medicine extract, 3-5 parts of lactic acid bacteria and 1-3 parts of an acid preparation. The feed additive provided by the invention significantly inhibits the expression of foot and mouth disease virus mRNA and inhibits the replication of foot and mouth disease virus; when the feed additive provided by the invention is added into daily feed for yaks, the foot-and-mouth disease of yaks can be effectively prevented and treated.

The preparation method comprises the following steps: mixing saccharomycetes, lactic acid bacteria and bacillus subtilis according to a ratio of 1: 1: 1 to obtain mixed bacteria; 0.25 g of mixed bacteria is taken and dissolved in 5 mL of sterile water for standby application; slicing and crushing the dried rhizoma polygonati, and sieving with a 10-mesh sieve to obtain rhizoma polygonati powder; the preparation method comprises the following steps: mixing water and rhizoma polygonati powder according to a liquid-material ratio of 10mL: 1g, sterilizing at 121 DEG C for 30min, cooling, adding mixed bacteria according to an inoculum size of 3% of the mass of rhizoma polygonati, culturing in a constant-temperature shaking table incubator at 120r. Min <-1 > for 72h at the culture temperature of 035 DEG C, sterilizing at 100 DEG C for 30min after fermentation, cooling and centrifuging to obtain rhizoma polygonati fermentation liquor, and storing at 4 DEG C. The prepared polygonatum sibiricum fermentation liquor is high in active ingredient content, has good cell safety, anti-oxidation, anti-inflammatory and whitening effects, and has the potential of being used for developing functional raw materials of traditional Chinese medicine cosmetics.

The invention discloses low-drunkenness type Baijiu distiller's yeast and a preparation method and application thereof. The prepared distiller's yeast can be used for producing baijiu, the physical and chemical indexes of the baijiu are good, fusel oil is reduced by 30%-57%, the metabolism speed of yeast is controlled by controlling the saccharification speed, and the generation of the fusel oil is reduced; meanwhile, lactic acid bacteria and bacillus subtilis of the distiller's yeast and bacteria cultured in a high-temperature stage can effectively inhibit the path of fusel oil metabolized by yeast, generation of fusel oil is further reduced, total ester and total acid are slightly increased basically, the content of total dibasic acid is increased by 10 times, and the content of dibasic acid diethyl ester is increased by 2 times. After the wine liquid is aged, drunkenness detection is carried out, and the comprehensive drunkenness Q is 0.6815-0.8325 and is far lower than a variety of commercially available white spirits (the comprehensive drunkenness is greater than or equal to 1).

The invention belongs to the technical field of traditional Chinese medicine fermentation, and particularly relates to dendrobium officinale fermentation liquor with a pancreatic lipase inhibition effect and a preparation method of the dendrobium officinale fermentation liquor. The dendrobium officinale fermentation liquor is prepared by performing enzymolysis on dendrobium officinale and inoculating lactic acid bacteria for fermentation, wherein the dendrobium officinale fermentation liquor obtained by inoculating fermentation lactobacillus mucus DSM 13273 or leuconostoc mesenteroides subsp. Mesenteroides XD054 has a better pancreatic lipase activity inhibition effect.

The invention belongs to the technical field of microorganisms, and particularly relates to probiotic characteristics of lactic acid bacteria and a relieving effect of a metagen of the lactic acid bacteria on pulmonary fibrosis. The invention discloses pediococcus pentosaceus ZFML001, and the preservation number of the pediococcus pentosaceus ZFML001 is CCTCC (China Center for Type Culture Collection) NO: M 2024601. The invention also provides a metagen prepared from the pediococcus pentosaceus ZFML001 at the same time, and provides an application of the metagen in preparation of a medicine for relieving or assisting in treatment of pulmonary fibrosis. The invention further provides the application of the metagen in preparation of a medicine for relieving or assisting in treatment of pulmonary fibrosis.

The invention relates to the technical field of daily chemicals, in particular to a preparation method and application of a sapindus mukorossi fermentation product with oil-controlling and dandruff-removing effects. The preparation method comprises the following steps: (1) drying soapberry in the air, separately powdering peel and seeds, adding 75-80 parts of water, performing ultrasonic extraction at 35 DEG C for 2 hours, then adding 0.5-0.9 part of dipotassium phosphate, sterilizing, and inoculating 3-5% of lactic acid bacteria liquid for fermentation; (2) after the fermentation is finished, adding 3-7 parts of sterilized aloe extract, 2-5 parts of mulberry leaf extract, 5-10 parts of tea bran extract and 6-9 parts of rose extract, adjusting the pH value to 5-7, inoculating 3-5% of schizosaccharomyces pombe, culturing for 24-36 hours at 28-30 DEG C, and introducing oxygen at 0.3-0.5 L / min; and (3) centrifuging at 10000r, and filtering through a sieve plate to obtain clear fermentation liquor, namely the sapindus mukorossi fermented product. The soapberry peel and the soapberry seeds are separately crushed, and the peel powder and the seed powder are mixed according to a ratio of 90%: 10% for use, so that the saponin content and the irritation are favorably controlled, and the use feeling is improved.

The invention relates to a method for regulating and controlling gene expression of lactic acid bacteria in shrimp acid fermentation based on systematic biology. The method comprises the following steps: constructing a gene regulation network of lactic acid bacteria by using whole genome data and a high-throughput sequencing technology comprising RNAseq; through clustering analysis and network reconstruction, transcription factors and enzymes of key metabolic pathways of lactic acid generation, acid resistance and glycometabolism are identified, and a dynamic regulation and control model of gene expression is established; a GeneNet or Cytoscape software tool is used, genomics, transcriptomics and metabonomics data of lactic acid bacteria are fused in a multi-layer network, a metabolism regulation and control system is constructed, and the metabolism regulation and control system comprises gene expression regulation and control and real-time change of metabolic flow; the method comprises the following steps: constructing a metabolic flux dynamic model of lactic acid bacteria based on a metabolic flux analysis MFA and a dynamic system modeling technology, and analyzing a mutual relation between a lactic acid generation path and a byproduct generation path; fBA is analyzed through flow equilibrium, and the metabolic network of lactic acid bacteria is optimized.

The invention relates to the technical field of saline-alkali soil improvement, and discloses a composite improved saline-alkali soil composition based on biomass charcoal and a preparation method thereof.Biomass charcoal, glucose, lactic acid bacteria, saccharomycetes, aluminum sulfate and a quaternary ammonium salt modifier are added into a stirrer to be stirred and mixed, stirring is conducted, and a mixture is obtained; and obtaining the composite improved saline-alkali soil composition based on the biomass charcoal. A quaternary ammonium salt group in the quaternary ammonium salt modifier can reduce the pH value of the soil and reduce the alkalinity of the soil, so that the salinization condition of the soil is improved, and quaternary ammonium salt can promote the activity of soil microorganisms and improve the fertility of the soil and the growth environment of crops; the siloxane is converted to be neutral by adjusting the pH value of the soil, the siloxane is beneficial to reducing the alkalinity of the soil, and a more suitable growth environment is provided for plants; wherein the contained boric acid group reduces the pH value of the soil by neutralizing alkaline substances in the soil, and has a relatively good improvement effect on the saline-alkali soil.

The embodiment of the invention discloses plant lactobacillus, selenium-enriched lactic acid bacteria and application of the plant lactobacillus and the selenium-enriched lactic acid bacteria in preparation of selenium-enriched food, the plant lactobacillus is inoculated into a sodium selenite solution, and the selenium-enriched lactic acid bacteria are obtained through culture. Adding the selenium-rich lactic acid bacteria into the pickled bamboo shoot brine containing the pickled bamboo shoots, and fermenting to obtain the selenium-rich fermented pickled bamboo shoots. The plant lactobacillus with high selenium conversion rate is obtained, the prepared selenium-enriched lactic acid bacteria have wide application, the selenium-enriched lactic acid bacteria can be applied to preparation of selenium-enriched food, the selenium content in the food is greatly increased, for example, the physicochemical properties of the selenium-enriched fermented pickled bamboo shoots prepared in the invention are improved compared with those of traditional pickled bamboo shoots, and the selenium-enriched fermented pickled bamboo shoots have good market prospects. Good oxidation resistance is realized.

The invention discloses a ganoderma lucidum fermentation liquid capable of improving the content of total triterpenes and immunity and a preparation method and application thereof, the preparation method comprises the following steps: heating a ganoderma lucidum liquid, and carrying out hot water extraction to obtain a ganoderma lucidum extracting liquid; heating the lucid ganoderma extracting solution to be boiled, after sterilization is finished, after the lucid ganoderma liquid is cooled, adding lactic acid bacteria powder in a specific proportion for fermentation to obtain lucid ganoderma fermentation liquid; and carrying out solid-liquid separation on the fermentation liquor to obtain fermentation supernate, and sterilizing the ganoderma lucidum fermentation liquor to obtain the sterilized ganoderma lucidum fermentation liquor. According to the preparation method disclosed by the invention, the content of total triterpenes in the ganoderma lucidum fermentation liquor is increased, the immunomodulatory activity of the ganoderma lucidum product can be enhanced, and the ganoderma lucidum product has relatively high effects of resisting oxidation, improving the activity of phagocytes, improving the activity of acid phosphatase, improving the immunity and the like, and has a wide application prospect and market potential.

The invention relates to the technical field of animal feeds, and provides an animal feed and a preparation method and optimization method thereof, and the optimization method of the animal feed comprises the following steps: determining candidate microorganisms and nutrition raw material formulas, establishing an optimization model, and solving to obtain an optimal microorganism formula and an optimal nutrition raw material formula; the animal feed comprises microorganisms such as lactic acid bacteria, saccharomycetes and bacillus and nutritional raw materials such as corn and soybean meal. By adopting a multilayer microcapsule technology, the survival rate of microorganisms in an acid environment is improved, the digestion and absorption efficiency is improved, and the nutritional condition and the milk production performance are improved; the invention further provides a corresponding preparation method, and the feed has the remarkable slow release characteristic and the effect of improving the feed utilization rate.

According to the probiotic agent for enhancing the immunity and improving the intestinal flora and the application of the probiotic agent, five specific different types of bifidobacterium strains are compounded, potential interaction exists among the five strains, and the five strains can cooperate with one another, so that the effect of enhancing the immunity and improving the intestinal flora is achieved. The bifidobacteria has the advantages that the bifidobacteria has synergistic interaction in the aspects of promoting the growth of immune organs and increasing the total quantity and diversity of intestinal flora, the bifidobacteria is matched with basic fermentation strains for yogurt fermentation, meanwhile, the composite prebiotics are added for synergistic yogurt fermentation, the quantity of the bifidobacteria at the end of a shelf life after the yogurt fermentation is finished is still not less than 1 * 10 < 8 > CFU / mL or 1 * 10 < 8 > CFU / g, and the content of the bifidobacteria is not less than the stability of lactic acid bacteria in the yoghourt is remarkably improved, so that the number of viable bacteria which enter the intestinal tract of a human body and play roles is remarkably increased, and the yoghourt has relatively good tolerance in the intestinal tract; the prepared yoghurt is fine and smooth in texture, rich in taste and rich in fragrance; in addition, the immune decline and intestinal flora disorder caused by cyclophosphamide (CTX) can be remarkably improved, and a positive effect on immunological enhancement is achieved.

The present invention relates to the screening of functional probiotics and its application, and specifically provides a strain of Lactobacillus casei RH99 that produces high extracellular polysaccharides and relieves vaginitis, and its preservation number is CGMCC No.32108. The Lactobacillus casei can inhibit the growth of Gardnerella, Candida albicans, and Peptostreptococcus and their biofilm generation ability; the extracellular polysaccharides produced by it have a significant promoting effect on the biofilm formation of Lactobacillus casei itself. RH99 is inoculated into the vagina, and its strain, the metabolites (exocellular polysaccharides) produced, and the core monosaccharide (D-mannitol) can all inhibit the colonization of pathogenic bacteria and promote the colonization of lactic acid bacteria; and RH99 can also relieve vaginitis by inhibiting inflammatory factors. It can be seen that RH99 can inhibit the growth of pathogenic bacteria + inhibit biofilm formation + inhibit pathogenic bacteria adhesion + inhibit the level of inflammatory factors through key core metabolites, and realize the all-round inhibition of four-in-one.

The invention belongs to the technical field of medicine and health care, and particularly relates to a preparation method of an American ginseng root water extract rich in rare ginsenoside and application of the American ginseng root water extract in improving the immune and anti-tumor direction. The preparation method comprises the following steps: step 1, carrying out superfine grinding on American ginseng, and sieving; step 2, adding the American ginseng powder into water, and then adding compound enzyme for enzymolysis to obtain enzymatic hydrolysate; s3, after the enzymolysis is finished, heating the enzymatic hydrolysate, extracting twice, and mixing after the extraction is finished; and S4, inoculating compound lactic acid bacteria into the mixed solution, fermenting to obtain a fermentation solution, blending, and sterilizing to obtain the American ginseng afterbiogen beverage. According to the invention, a new physical-biological combined process is adopted, and crushing, enzymolysis, extraction, fermentation and blending are carried out, so that the American ginseng metagen beverage rich in rare ginsenoside is obtained, and the rare ginsenoside has a remarkable anti-tumor effect and higher activity.

The invention belongs to the technical field of preparation of probiotic products, and relates to a lactic acid bacteria microcapsule as well as a preparation method and application thereof. The method comprises the following steps: uniformly mixing sodium alginate, modified montmorillonite and sterile water, cooling, adding a prepared lactic acid bacteria seed solution, and fully and uniformly mixing to form an emulsion; spraying the emulsion into a calcium chloride solution by adopting an extrusion method, stirring and curing to obtain microcapsules; and culturing the prepared microcapsule in a lactic acid bacteria liquid culture medium to obtain the lactic acid bacteria microcapsule after the culture is finished. The lactobacillus is effectively protected by adopting an extrusion-method single coating and post-coating culture process, so that the stability of the lactobacillus to a humid and hot environment and the gastrointestinal stress resistance are improved, and meanwhile, the release performance of the lactobacillus in the gastrointestinal tract is ensured. The problem that an existing microcapsule coating technology cannot achieve balance between improvement of the hygrothermal stability and gastrointestinal stress resistance of the probiotics and guarantee of good release performance of the probiotics in the gastrointestinal tract is solved.

The invention belongs to the technical field of hair care products, and particularly relates to composite acid gel for scalp care and a preparation method thereof. Salicylic acid, glycolic acid, piroctone azelate olamine salt, echinacea purpurea-lactic acid bacteria yeast, an emulsifier and water are combined, wherein the echinacea purpurea-lactic acid bacteria yeast is prepared by performing enzymolysis on echinacea purpurea through bacillus subtilis protease and then performing aerobic fermentation with lactic acid bacteria. Salicylic acid, glycolic acid and azelaic acid in the composite acid gel are compounded, so that stimulation of salicylic acid is weakened, and the effect of dissolving hair follicle grease is reserved; glycolic acid and azelaic acid are added to accelerate keratin metabolism, regulate sebum secretion and create safe and healthy shielding for the scalp environment, and polysaccharide and flavonoid compounds in the echinacea purpurea-lactic acid bacteria leavening are beneficial to exerting the anti-oxidation and immune regulation effects of the echinacea purpurea-lactic acid bacteria leavening. Therefore, the composite acid gel provided by the invention is green, safe and non-irritant, the preparation method is simple and convenient, and the composite acid gel can be used for inhibiting bacteria and reducing scalp inflammation.

The present disclosure is a screening procedure allowing the identification of inhibitors of cell envelope proteases (CEP) in pathogenic bacteria by using lactic acid bacteria (LAB). The screening platform can be used to screen compound libraries, and thus generate a high throughput evaluation of drug candidates, and as exemplified below one screening of 6808 compounds has resulted in the identification of 20 compounds inhibiting the CEP of Lactococcus lactis.

The invention provides a microecological preparation with an immunological enhancement function and application of the microecological preparation in antibiotic-free breeding of livestock and poultry, and relates to the technical field of agricultural biology. The microecological preparation with the immunological enhancement function comprises a probiotic component, a prebiotics component and an immunological enhancement component, and the probiotic component, the prebiotics component and the immunological enhancement component are respectively composed of lactic acid bacteria, bacillus, clostridium butyricum, fructo-oligosaccharide, yeast beta-glucan, astragalus polysaccharide and chitosan oligosaccharide. Wherein the lactic acid bacteria accounts for 30%-40%, the bacillus accounts for 20%-30%, the clostridium butyricum accounts for 10%-20%, the fructo-oligosaccharide accounts for 5%-10%, the yeast beta-glucan accounts for 3%-8%, the astragalus polysaccharide accounts for 2%-5%, and the chitosan The probiotics can enhance immunity and regulate intestinal tracts at the same time, the effect is improved by 40% or above compared with common probiotics, animals suffer less illness and grow fast, flora can resist high temperature and gastric acid through a special protection technology, the loss of viable bacteria is less than 5% after the probiotics are stored for 3 months, and the probiotics are stable by two times compared with conventional products.

The invention discloses a Weissella sinus strain for improving the fermentation flavor of American ginseng and application of the Weissella sinus strain. According to the present invention, through the comprehensive evaluation of a variety of lactic acid bacteria, including the acid production capacity, the chroma regulation and the flavor characteristic analysis, the optimal strain for the American ginseng fermentation is finally screened, and the strain shows the significant flavor regulation advantage. On one hand, characteristic earthy smell substances of American ginseng can be completely degraded, and the bad flavor of the raw materials is effectively improved; on the other hand, a compound with a fruity flavor characteristic can be specifically generated, and a fermentation product is endowed with a remarkable fragrant and sweet flavor. In addition, the strain disclosed by the invention maintains proper acid production capacity and chroma stability, and the flavor quality is remarkably improved on the premise of ensuring the fermentation efficiency. Due to the characteristics, the strain becomes an ideal fermentation strain for developing a high-quality fermented American ginseng product.

The invention relates to the technical field of food, and provides a pea complete protein component and a preparation and identification method thereof.The preparation method of the complete protein comprises the steps that pea kernel powder and water are mixed and then subjected to first solid-liquid separation, and first clear liquid is obtained; adjusting the pH value of the first clear liquid to 5.8-6.2 to obtain a protein mixed liquid; adding lactic acid bacteria into the protein mixed solution, and standing for 1-3 hours at the temperature of 10 DEG C or below for layering; removing a bottom layer material of the layered material to obtain a residual material, and carrying out second solid-liquid separation on the residual material to collect a precipitate; performing first drying on the precipitate to obtain protein isolate; mixing the separated protein and water, and performing third solid-liquid separation to obtain a second clear liquid; and separating the second clear liquid through an ultrafiltration membrane, and performing secondary drying on the obtained percolate to obtain albumin, namely the complete protein. The method disclosed by the invention is green and environment-friendly, and the extracted complete protein is good in balance and meets nutritional requirements.

To provide a phagocytotic promotor of macrophage by a lactic acid bacterium, an activator of a plasmacytoid dendritic cell (pDC) by a lactic acid bacterium, and an activator of a myeloid dendritic cell (mDC) by a lactic acid bacterium.SOLUTION: A phagocytotic promotor of macrophage, an activator of a plasmacytoid dendritic cell (pDC), an activator of a myeloid dendritic cell (mDC) can be provided by including Lactobacillus panisapium M1 strain (NITE BP-03106) as an active ingredient.SELECTED DRAWING: None

The invention discloses a preparation technology of directional debitterizing yellow tea, and belongs to the technical field of tea processing. Fresh tea leaves are used as raw materials, the yellow tea is prepared through the processes of pretreatment, fermentation, fixation, rolling, heaping for yellowing and drying, during fermentation, a compound bacteria solution and the spread and aired fresh leaves are mixed and fermented, strains in the compound bacteria solution comprise lactic acid bacteria, saccharomycetes and bacillus, and through the synergistic effect of the lactic acid bacteria, the saccharomycetes and the bacillus, the yellow tea is prepared. Balancing of directional degradation of bitter and astringent substances and generation of flavor substances is achieved, high-quality yellow tea is prepared through efficient debitterizing, the stage heaping yellowing technology is adopted, primary heaping and secondary heaping are adopted for two-stage heaping yellowing, the quality of the yellow tea is enhanced, and poor taste caused by excessive heaping yellowing is avoided. According to the finished tea prepared by the preparation process, the content of tea polyphenol is reduced to 18%-20% through detection, the content of amino acid reaches 4.5%-5.0%, bitter taste and astringent taste are relieved, sweet taste is added, and the taste is mellow.

The invention discloses a rice milk coffee beverage and a preparation method thereof, and belongs to the technical field of coffee beverages. The preparation method of the rice milk coffee beverage comprises the following steps: mixing brown rice milk and glutinous rice milk to obtain mixed rice milk; adding a compound enzyme into the mixed rice milk, and carrying out enzymolysis and pre-gelatinization to obtain pre-gelatinized rice milk, wherein the compound enzyme comprises cellulase and amylase; gelatinizing the pre-gelatinized rice milk to obtain gelatinized rice milk; whey protein powder is added into the gelatinized rice milk for hydration, and homogenization treatment is performed to obtain homogenized rice milk; adding compound bacteria into the homogenized rice milk, and fermenting to obtain fermented rice milk, wherein the compound bacteria comprise saccharomycetes and lactic acid bacteria; the fermented rice milk and coffee are mixed to obtain the rice milk coffee beverage. The prepared rice milk coffee beverage can replace milk to improve bitterness and roughness of coffee, people with lactose intolerance can be cared, discomfort caused by lactose intolerance is reduced, and the rice milk coffee beverage has unique flavor.

The invention discloses a method for promoting lactobacillus activity enhancement, which comprises the following steps: S1, amplifying a target gene corresponding to an LPxTG structural protein, and amplifying a target gene corresponding to a signal peptide; s2, obtaining a target gene connected with the LPxTG structure and the signal peptide; s3, connecting the target gene obtained in the step S2 with a plasmid gene sequence to construct a recombinant expression vector; s4, introducing the recombinant expression vector obtained in the step S3 into escherichia coli BL-21 so as to increase the number of the recombinant expression vector; s5, introducing the recombinant expression vector obtained in the step S4 into lactobacillus plantarum C8 to obtain a recombinant strain; and step S6, co-culturing lactobacillus plantarum C8 and the recombinant strain obtained in the step S5. The lactobacillus plantarum C8 has the advantages that the whole lactobacillus plantarum C8 and the recombinant strain have better survival condition and adhesiveness in gastric juice and intestinal juice.

The present invention relates to the technical field of feeds and particularly relates to a piglet weaning transition feed containing lactic acid bacteria and saccharomycetes compound fermented soybean meal and a preparation method thereof, and the piglet weaning transition feed comprises the following components in parts by weight: 15-20 parts of compound fermented soybean meal, 45-50 parts of corn, 10-15 parts of wheat, 5-7 parts of fish meal, 3-5 parts of whey powder, 3-4 parts of vegetable oil, 1-2 parts of an amino acid supplement and 2-3 parts of a mineral premix, 1-2 parts of a vitamin premix and 1.5-2 parts of a calcium-phosphorus supplement; wherein the compound fermented soybean meal is prepared by performing compound fermentation on soybean meal through lactic acid bacteria and saccharomycetes, and a complementary and symbiotic microbial ecological system is formed by carefully selecting and optimizing the combination proportion of three lactic acid bacteria and two saccharomycetes, so that the fermentation efficiency and stability are improved, and the functionality of a fermentation product is enhanced. Different strains complement each other in the aspects of carbohydrate metabolism, pH tolerance and functional characteristics, and nutrition competition is reduced.

The invention belongs to the field of food fermentation, and particularly discloses a fermentation process of pickled vegetables in old jars, which comprises the following steps: selecting fresh vegetables, carrying out irradiation sterilization pretreatment, carrying out primary micro-aerobic fermentation by using a compound bacteria solution containing specific microorganisms, and carrying out secondary anaerobic fermentation by using a steeping liquor with a specific ratio. In the process, the vegetables are diversified, the compound bacteria liquid is rich in various beneficial microorganisms such as halophilic archaea, lactic acid bacteria and yeast, the steeping liquor 1 and the steeping liquor 2 respectively optimize the fermentation environment and are rich in nutritional ingredients and flavor substances such as lactic acid bacteria exopolysaccharides, tea polyphenols and selenium-enriched yeast powder, and the taste and fragrance of the pickled vegetables are improved. Besides, according to the process, by accurately controlling the fermentation temperature, humidity and time, the fermentation period is remarkably shortened, meanwhile, the use of chemical additives is avoided, and the safety and health of the pickled vegetables are guaranteed; the prepared pickled vegetables in old jars are delicious in taste, rich in fragrance, short in fermentation time and free of chemical additives, and the requirements of consumers for high-quality and healthy foods are met.

The invention belongs to the technical field of preparation of postbiotics, and discloses a preparation method and application of lactic acid bacteria fermented Chinese chestnut protein postbiotics for improving systemic chronic inflammations, Chinese chestnuts are subjected to shelling, grinding and degreasing treatment, and degreased Chinese chestnut powder is obtained; resuspending the degreased Chinese chestnut powder in distilled water, adjusting the pH value, and stirring; performing solid-liquid separation and magnetic stirring; carrying out solid-liquid separation, resuspending and adjusting the pH value; dialyzing to obtain Chinese chestnut protein powder; mixing with glucose powder, and redissolving in distilled water; and mixing with lactic acid bacteria and carrying out aerobic fermentation to obtain the Chinese chestnut protein metaplasm product. The bioactive peptides contained in the Chinese chestnut protein metaplasm prepared by the method have potential anti-inflammatory activity, can effectively inhibit proinflammatory medium secretion of macrophages and intestinal epithelial cells, and improve glucose and lipid metabolism disorder and intestinal barrier function damage related to chronic inflammation of a body. The bioactive peptide contained in the postbiotic product disclosed by the invention has an anti-inflammatory effect.