3903 results about "Lactic acid bacterium" patented technology

The invention discloses a composite enzyme beverage and a preparation process method thereof. The composite enzyme beverage is prepared from five cereals, fruits and vegetables, edible plants and edible fungi as main raw materials through common fermentation; three-step fermentation is realized by using microzyme, acetic acid bacteria and lactic acid bacteria; and substep fermentation for different strains is adopted at different stages in the manufacturing process, so that the demands of a human body on the efficacies of health and nutrition are met. According to the composite enzyme beverage disclosed by the invention, the mixture ratio of the raw materials is more gentle, more pure natural organic raw materials are used and the three-step fermentation is realized by the microzyme, the acetic acid bacteria and the lactic acid bacteria; and the composite enzyme beverage retains original vitamins and carbohydrate of the fruits and vegetables, is rich in various B-group vitamins and various amino acids, is a nutrient necessary for eliminating the human fatigue and particularly benefits people suffered from chronic disease syndrome. Particularly after coarse grains are fermented, insoluble dietary fibers can be dissolved into small molecular substances which are easily digested and absorbed by the human body, and thus the digestion and intake of nutritive substances by the human body can be enhanced.

Compositions including a non-pathogenic lactic acid-producing bacteria, such as a Bacillus species, spores or an extracellular product of B. coagulans, formulated for oral administration to the intestinal tract for inhibiting bacterial gastrointestinal infections are described. Methods and systems using the compositions for treating gastrointestinal infections, particularly sudden infant death syndrome (SIDS) are also disclosed.

A nutritional composition is described for prevention or treatment of an immune condition. The composition includes at least vitamin E, vitamin C, vitamin B6, folic acid, vitamin B12, copper, zinc, selenium, fructo-oligosaccharides and / or gum acacia, a probiotic lactic acid bacterium. For example, in an embodiment it comprises per 300 g: 150 IU Vitamin E, 120 mg Vitamin C, 2 mg Vitamin B6, 400 ug Folic acid, 3.8 ug Vitamin B12, 1.5 mg Copper, 15 mg Zinc, 100 ug Selenium, 3 g Fructo-oligosaccharides and / or gum acacia, 10E10 cfu ST11 lactobacillus. Also disclosed are a method for making the nutritional composition, a method for manufacturing a functional food or medicament; and a method of prevention or treatment of an immune condition by administering an effective amount of the composition, functional food or medicament.

The invention relates to a fermentation method for production of nutritionally balanced natural fruit enzymes. The method comprises the following steps: (1) pretreatment on raw materials; (2) a first bacterium inoculation: inoculating a saccharomyces cerevisiae liquor with concentration of 106 CFU / ml into a pulp according to an inoculation proportion of 5%; (3) early stage fermentation; (4) a secondary bacterium inoculation: after the early fermentation, inoculating acetic acid bacterium liquid and lactic acid bacterium liquid in a weight ratio of 1:1 for a secondary bacterium inoculation according to an inoculation amount of 5%; (5) middle-term fermentation; (6) coarse filtration; (7) secondary fermentation; (8) ripening; and (9) fine filtration to obtain the natural fruit enzyme. The fermentation method provided by the invention through low-temperature sterilization and strict control on the fermentation temperature retains the enzyme activity to the maximum; and twice inoculation of dominant bacteria effectively inhibits breeding of other infectious microbes, so as to realize stable product quality.

The invention discloses a compound microorganism liquid bacterial fertilizer for preventing plant diseases and insect pests and a preparation method of the compound microorganism liquid bacterial fertilizer and belongs to the technical field of microorganisms. The bacterial fertilizer comprises raw materials of, by weight, 10-20 parts of paprika powders, 10-20 parts of chilli powders, 5-15 parts of ginkgo leaf powders, 5-15 parts of chinaberry powders, 5-15 parts of lightyellow sophora root powders, 20-40 parts of mashed garlic, 40-80 parts of molasses, 4-8 parts of soybean meal, 5-15 parts of calcium sulfate, 5-15 parts of magnesium chloride, 5-10 parts of salt, 5-15 parts of photosynthetic bacterium strains, 5-15 parts of saccharomycete strains, 5-15 parts of lactic acid bacterium strains, 5-15 parts of streptomyces jingyangensis strains, 5-15 parts of bacillus subtilis strains and 800 parts of water. According to the compound microorganism liquid bacterial fertilizer, probiotic floras which are screened and are capable of coexisting are used for fermenting plant leaching agents of pepper, chilli, ginkgo leaves, chinaberry, lightyellow sophora roots and garlic, the plant leaching agents are capable of expelling and killing the insect pests and harmful bacteria, the compound microorganism liquid bacterial fertilizer has the advantages of being high in content of live bacteria of probiotics, high in activities, high in stability, small in using amount of plant materials, fast in acting effects, non-toxic and environment-friendly, and has wide application prospects in agricultural planting.

The invention relates to a prebiotic peptide biological feed additive and a preparation method and application thereof. The prebiotic peptide biological feed additive is prepared by mixing and fermentation of seed solution with one or more of bacillus, yeast and lactic acid bacteria in plant protease hydrolysate. After animal and plant protein is subjected to enzymolysis at constant or changeable temperature, the seed solution with one or more of bacillus, yeast and lactic acid bacteria is added for fermentation according to a certain proportion, and the fermented solution is concentrated and dried to obtain a powdery product. The problems of high content of bitter peptides and poor palatability of enzymolysis processes using acid, alkali and protease can be solved. The immune function of animals can be stimulated by active peptides in the prebiotic peptide biological feed additive, the immunity of the animals can be improved, the intestinal environment of the animals can be improved by probiotics, the resistance of the animals to a variety of diseases can be enhanced, the feed conversion rate can be increased, and the breeding cost can be reduced.

The invention discloses a charcoal-based microbial soil conditioner. The charcoal-based microbial soil conditioner comprises, by weight, 80-85 parts of charcoal, 10-13 parts of a composite microbial flora and 1-2 parts of an oxidized starch adhesive, and the composite microbial flora comprises photosynthetic bacteria, actinomyces, lactic acid bacteria, microzyme, Bacillus subtilis and Bacillus thuringiensis. The invention also discloses a preparation method of the charcoal-based microbial soil conditioner. The combined effect of charcoal and microbes alleviates soil acidification, reduces the unit weight of soil and increases the permeability and the water content of soil; soil organic matter formation is promoted to improve the validity of mineral elements; and the abundance of microbes at the rhizosphere of crops is cultivated to improve the rhizospheric environment of plants in order to make pathogen microbes difficultly grow and breed, so the insect diseases of crops is reduced, and soil heavy metal pollution is alleviated and restored.

The invention describes a microorganism of the order of lactic acid bacteria or an analogue, fragment, derivative, mutant or combination thereof, wherein the microorganism, or analogue, fragment, derivative, mutant or combination thereof can coaggregate with Streptococcus pyogenes.

According to the invention there is provided a strain of lactic acid bacteria of the genus Lactobacilli obtainable by isolation from resected and washed feline gastrointestinal tract having a probiotic activity in animals. Methods of use and compositions comprising the Lactobacilli of the present invention are also provided.

Processes to produce microorganisms that can be incorporated into a microbial-based product that results in high viable cell yields and shelf-stable products are disclosed. These microbial-based products are useful for inhibiting pathogenic growth and as a food additive. A preferred microorganism is a lactic acid producing bacteria. In one embodiment, the process comprises inoculating a lactobacillus fermentation medium with lactic acid producing bacterial cells, harvesting the lactic acid producing bacterial cells at mid to late log phase, concentrating the lactic acid producing bacterial cells, and preserving the lactic acid producing bacterial cells at a concentration of at least 5×109 cfu / ml.

A method for regulating fermentation of organic material, such as cocoa beans and pulp is disclosed. The method includes adding at least one strain of lactic acid bacteria and / or acetic acid bacteria to the organic material. Optionally, at least one strain of yeast is added. Bacterial strains and compositions, useful in the described method, are also disclosed. The use of the described starter cultures and compositions permits faster fermentation, fermentations with targeted population dynamics and succession of microorganisms, and fermentations with targeted levels of both desirable and undesirable metabolites.

The invention relates to a microbe soil repairing additive and process for preparation. The process for preparation comprises that liquid microbe fertilizer containing photosynthetic bacteria, bacillus, actinomycete and lactic acid bacteria are absorbed to one or a plurality of natural organic fertilizers as grass peat and zeolite powder or the like, to be mixed proportionally. The invention further relates to the application of the microbe soil repairing additive for repairing soil. The invention can lead useful microbe into soil, uses microbe technique to transfer, absorb, degrade, and convert the contaminant in soil, adjust soil pH value, eliminate soil blocking, and restrain soil-borne disease, thereby improving soil structure, to return soil to original ecologic structure. And the viable bacteria group of the invention is planted in plant root, to quickly grow and deeply insert plant root, thereby improving yield, quality and level of agricultural product, as one ideal fertilizer of organic agriculture, with wide application in agriculture continuous development.

The invention relates to a method for producing natural cream-vanilla condiment, which comprises that using lactic acid bacteria to ferment the fresh milk or milk powder, adding cream into the fermented product, using biological enzyme as protein enzyme and / or lipase to process enzymolysis, heating, concentrating, and emulsifying. The invention can improve 100-500 times of the contents of butanedione, dimethylsulfide or the like. And the inventive condiment can be directly added into food.

A high flux of metabolites from pyruvate to 2,3-butanediol in Lactobacillus plantarum was achieved through genetic engineering. Substantial elimination of lactate dehydrogenase activity in the presence of heterologously expressed butanediol dehydrogenase activity led to 2,3 butanediol production that was at least 49% of the total of major pyruvate-derived products.

The invention discloses a fermented tea beverage and a preparation method thereof. The fermented tea beverage is characterized by comprising the following components in percentage: 20-80% of tea extract, 0.01-0.5% of sodium D-isoascorbate or vitamin C, 0.01-0.5% of citric acid, 0-30% of syrup or substituted sugar and 0-79.98% of purified water; and fresh tea leaves are extracted to obtain the tea extract. The preparation method is characterized by comprising the following steps: uniformly stirring the components in proportion, and sterilizing at the temperature of 85-100 DEG C for 1-10 minutes; after the sterilization, cooling a tea slurry to 20-45 DEG C; inoculating lactic acid bacteria to the sterilized and cooled tea slurry based on a ratio of 10<3>-10<9> cfu / ml; and fermenting at 25-45 DEG C for 6-48 hours, wherein the fermentation ends when the pH value is 3.5-5.0. The invention has the following advantages: (1) natural gentle acidity can be produced to effectively remove the bitter of the tea juice; (2) by fermenting with the lactic acid bacteria, the content of the amino acid in the tea slurry can be increased by over 10%, flavor substances can be increased by over 30%, and the flavor and mouthfeel of the product are effectively improved; (3) nutrient components such as vitamin and the like in the tea leaves are fully maintained; and (4) the shelf life of food is prolonged, and spoilage is prevented.

This invention relates to a process for the production of a cultured savory base by hydrolyzing a protein-containing material using a combination of at least one enzyme with at least one thermotolerant lactic acid bacteria strain selected for its ability to provide a glutaminase activity. The invention also relates to an isolated strain of thermotolerant lactic acid bacteria strains selected for their ability to provide a glutaminase activity and their use for preparing a seasoning or flavor for culinary or petfood products.

The invention discloses a premix feed for relieving astriction of sows, which is prepared by mixing 5 to 7 weight parts of premix feed and 93 to 95 weight parts of main ingredient. The premix feed comprises the following components in part by weight: 0.8 to 1 part of liquoric root, 0.8 to 1.0 part of Bacillus subtillis and lactic acid bacteria preparation, 0.3 to 0.6 parts of antibacterial peptide and 1.2 to 1.6 part of choline chloride. The premix feed can relieve astriction of sows by adding a raw material with high crude fiber content or adding mineral substance aperients, microecological preparation, functional oligosaccharide, dietary fibers, antibacterial peptides and various green additives, thereby improving the production performance of sows.

The invention discloses a method for improving the effect of medicinal liquor. The method comprises the following steps: a, crushing Chinese medicinal materials prepared according to specific effects, adding water, blending uniformly, and fully soaking the medicinal materials for more than 48 hours under the condition that the water content of the medicinal materials is 60 to 70%; b, melting a proper amount of honey by using warm water, preparing liquid by using the honey and a proper amount of probiotic composition, and adding the liquid into the soaked Chinese medicinal materials, wherein the probiotic composition consists of biological germs for promoting strong fermentation of the Chinese medicinal raw materials and decomposing, inhibiting and killing pathogenic bacteria groups; c, stirring once every day, adding white spirit of more than 65 degrees after fermenting for seven days, and soaking. The probiotic composition is characterized by being prepared from a photosynthetic bacteria group, a lactic acid bacteria group, a yeast group, a gram-positive actinomycete group, a filamentous bacteria group of a fermentation series, bifidobacteria, bacilli and the like. The method overcomes the defects of low effect and serious waste of the conventional medicinal liquor.

or continuous process for producing heterologous peptides, polypeptides or proteins in lactic acid bacteria, comprising the steps of (i) constructing a recombinant lactic acid bacterium comprising a nucleotide sequence coding for the heterologous peptide, polypeptide or protein, and appropriate regulatory nucleotide sequences such as regulatable promoters and signal peptides to control the expression of the coding sequence and the secretion of gene product, (ii) cultivating the recombinant bacterium under or continuous cultivation conditions to express the gene, and (iii) harvesting the recombinant bacterium or the gene product. Preferably, the cultivation medium is a chemically defined or synthetic medium optionally supplemented with yeast extract.

The invention relates to a method for producing a biologic feed from cassava lees, which comprises the following steps: preparing a lees culture medium; adding a mixture of a Trichoderma koningii suspension, a Trichoderma viride cladosporium suspension, a lactic acid bacterium seed liquid and a microzyme seed liquid to the lees culture medium for anaerobic culture and fermentation; and finally drying to obtain the biologic feed. The method has the advantages of low energy consumption, low cost, short production cycle and high production efficiency, realizes the recycling of resources and reduces the emission of pollutants. Besides, the invention shows that cassava lees can be used as the material for manufacturing a fermented feed and can improve the nutrition of the base material, a biologic feed which is rich in yeast, lactic acid bacteria and organic acid can be obtained, and the biologic feed can be used as the coarse feed for breeding cows and the like on an animal farm.

The invention relates to a compound microbial preparation for treating black and odorous rivers through strengthened calcium nitrate and a preparation method thereof. The use amount of calcium nitrate is reduced, and the time for treating the black and odorous rivers is shortened. The compound microbial preparation is prepared from 40-80 parts of thiobacillus denitrificans and thiocapsa roseoppersicina mixed powder, 10-60 parts of composite bacillus subtilis powder and 10-60 parts of yeast and lactic acid bacterium mixed powder. By means of the preparation, sulfide in black and odorous bottom sludge, organic matter and strains suitable for growing in the black and odorous bottom sludge are screened and degraded in a targeted mode; by means of thiobacillus denitrificans with efficient hydrogen sulfide degrading and denitrification capacity, sulfur-oxidizing bacteria of thiocapsa roseoppersicina with efficient hydrogen sulfide and ammoniacal nitrogen degrading capacity, bacilli rich in protease, amylase, cellulose and other enzyme systems, yeast and lactic acid bacteria, a small amount of calcium nitrate and the compound microbial preparation are thrown into the black and odorous rivers so that water quality of the black and odorous rivers can be quickly improved, the black and odorous bottom sludge can be removed quickly, and the thickness and the organic matter content of the bottom sludge can be quickly reduced.

The present invention relates to a vaccine for the prevention of lactic acidosis in a vertebrate, said vaccine comprising at least one isolated microorganism, or fragment or fragments thereof, wherein said microorganism is capable of producing lactic acid within the gut of said vertebrate, and wherein said microorganism is selected from the group consisting of: Clostridium-like species, Prevotella-like species, Bacteroides-like species, Enterococcus-like species, Selenomonas species, non-dextran slime producing Streptococcus species and non-slime producing lactic acid bacterial isolates.

The invention discloses a direct feeding viable bacteria preparation, wherein the viable bacteria content for each composition being, bacillus 1.0X10#+[9]-25X10#+[9]cfu / g, saccharomycete 1.0X10#+[9]-10X10#+[9]cfu / g, lactic acid bacteria 1.0X10#+[8]-10X10#+[8]cfu / g, the invention also discloses the process for preparing the viable bacteria preparation.

The invention relates to the field of vegetable processing, provides a processing method of wild vegetable pickles, and particularly relates to processing and products of pteridium aquilinum, aralia elata seem and acanthopanax senticosus pickles. The processing method comprises the following steps: (1) selecting materials; (2) preparing saline water; (3) protecting the color and keeping the crispness; (4) filling into a jar; (5) inoculating lactic acid bacteria; and (6) sealing and pickling. The invention relates to a processing process and a product research of the wild vegetable pickles; the storage period can be prolonged and the balance between seasons is promoted to meet the requirements of different regions and crowds; meanwhile, a novel product can be developed to increase economic profits of enterprises. The pickles prepared under the process condition are good in quality and have the main characteristics that the pickles are fermented by the pure lactic acid bacteria, are low in salt and nitrite, fresh and crispy in mouth feel, sour and delicious in taste and less in loss of nutritional ingredients, and have the typical characteristics of Sichuan pickles; the requirements of consumers are met and the disadvantages of salting of traditional wild vegetables are solved. The wild vegetable pickles can be stored for 8-12 months under pot storage and low temperature conditions.

The invention discloses a feed additive for conditioning intestines of animals and belongs to the technical field of feeds. The additive comprises probiotic, oligosaccharide, plant polysaccharide and an adsorbent, wherein the probiotic is a composition of bacillus licheniformis or bacillus subtilis, butyric acid bacteria and lactic acid bacteria. According to the feed additive for conditioning the intestines of the animals, a plurality of components are compatible, so that the feed additive can play a role from different angles such as improvement on the intestinal micro ecology environments, repair of intestinal immune systems, anti-inflammation, anti-virus and adsorption of toxin; the sub-health status of the animals during breeding can be solved; especially, the feed-mixed diarrhea phenomenon of poultry can be improved; the disease resistance of the poultry is improved; the feed utilization rate is increased; the feed meat ratio is reduced; the production performance is improved.

The invention discloses a soybean sauce employing multi-strain starter propagation and a production technology thereof. By adopting the method, the starter propagation process of a plurality of strains for naturally brewed soybean sauce is simulated, and yeast, lactic acid bacteria and composite aspergillus oryzae are adopted for starter propagation in common. The multi-strain starter propagation comprises the following steps: inoculating 0.1-0.5% of aspergillus oryzae and a koji material containing 10<3>-10<5> yeasts per gram at the initial starter propagation stage according to the weight of the raw material; and inoculating a koji material containing 10<3>-10<5> yeasts per gram after loosening koji for the first time. The yeast quantity in finished koji is 10<5>-10<7>cfu / g, the lactobacilli count is 10<5>-10<7>cfu / g, and the ratio of the lactic acid bacteria to the yeasts is (20:1) to (1:1) by collaborative symbiotic roles of three brewing microorganisms in the starter propagation process, flavor microorganisms (lactic acid bacteria and yeasts) with a proper proportion are provided for the fermentation stage of the soybean sauce, and the flavor of the soybean sauce product is finally enhanced.

The invention belongs to the technical fields of feeds, enzyme preparations and microbial fermentation, and particularly relates to a preparation method of zymohydrolysis concentrated silage special for sows. The preparation method comprises the following steps of cutting short fresh pasturage or tuberous roots and stems, performing mashing, inoculating lactic acid bacteria, and performing natural enzymolysis and anaerobic fermentation so as to obtain silage; mixing one or more of cake meal, food processing by-products and cereals, with clean water and molasses syrup, inoculating lactic acid bacterium liquid, and performing natural enzymolysis and anaerobic fermentation so as to obtain fermented concentrated wet basis; and inoculating oxygen consumption fermentation bacterium liquid into a concentrated silage fermented substrate, and performing natural oxygen consumption fermentation so as to obtain the zymohydrolysis concentrated silage special for sows. The zymohydrolysis concentrated silage is rich in fermented cellulose lactic acid, calcium lactate, small peptide, amino acid, vitamins and probiotics, has the characteristics of being high in nutrition, easy to digest, good in palatability and the like, and can obviously increase the lactation quantity of the sows, obviously increase the food consumption and notably reduce the constipation of the sows.

The invention mainly relates to a method for preparing a protein feed by fermentation on straw and cake using aerobic mixed bacteria. The method for preparing a protein feed by fermentation on straw and cake using aerobic mixed bacteria is mainly characterized by comprising the following steps of: uniformly stirring straw, cake, wheat bran and corn flour, and enabling the water content thereof toachieve 50-60%; adding ammonium sulphate having an amount of 0.5-4% relative to the total amount of dry materials and urea having an amount of 0.5-3% relative to the total amount of dry materials; adjusting pH to 5.5-6.5 by 1% lime water; joining the raw materials in bacterial solution having an amount of 8-12% relative to the total amount of dry materials, obtained by mixing geotrichum candidum,lactic acid bacteria and bacillus licheniformis, and having a ratio by weight of 50-60% of geotrichum candidum to 30-35% of lactic acid bacteria to 5-10% of bacillus licheniformis; and fermenting for18-24 hours in a ventilation condition at a temperature of 28-32 DEG C, so as to prepare the fermented protein feed. The method for preparing a protein feed by fermentation on straw and cake using aerobic mixed bacteria disclosed by the invention utilizes modern bio-technology, optimizes the best combined bacteria, has simple and convenient production process, saves equipments and energies, and reduces the needed investment.