The invention provides intestinal flora microorganisms for evaluating sexual precocity of children. The method comprises the following steps of: comparing intestinal flora data of an object with intestinal flora data of healthy children; and mainly analyzing the relative abundance of streptococcus, neisseria, haemophilus, porphyromonsginglivlis, megacoccus, phascolarctobacterium, clostridium, coprococcus and prevotella, wherein the relative abundance of the streptococcus, the neisseria, the haemophilus and the porphyromonsginglivlis is reduced, and the relative abundance of the megacoccus, thephascolarctobacterium and the clostridium is increased. Therefore, the streptococcus, the neisseria, the haemophilus, the porphyromonsginglivlis, the megacoccus, the phascolarctobacterium and the clostridium have higher specificity and sensitivity. Therefore, the streptococcus, the neisseria, the haemophilus, the porphyromonsginglivlis, the megacoccus, the phascolarctobacteriumand the clostridiumhave higher specificity and sensitivity. In the process of evaluating the sexual precocity of children, detection and research results are compared to evaluate the level change of intestinal flora microorganisms of children, and whether the risk of the sexual precocity exists or not is judged according to the level change of the intestinal flora microorganisms.