Use of Prevotella spp. in identifying and/or differentiating individuals of different ethnic groups
A technology of Prevotella and Prevotella intermedia is applied in the application field of Prevotella in identifying and/or distinguishing individuals of different ethnic groups, and can solve the problem of undisclosed applications of Prevotella.
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Embodiment 1
[0062] Example 1 Using the relative abundance of Prevotella in individual feces to identify a subject as Tibetan or Han
[0063] (1) determining the abundance of said microbial marker in said individual's stool sample:
[0064] 1) Collect the feces of the individual to be tested, and extract the DNA of the microorganisms in the feces:
[0065] Collect the feces of the individual to be tested, draw 1000ul of CTAB lysate into a 2.0ml EP tube, add lysozyme, add about 500ul of the sample into the lysate, put it in a 65°C water bath, and mix it upside down several times during the process to fully lyse the sample. Centrifuge to get the supernatant, add phenol (pH8.0): chloroform: isoamyl alcohol (25:24:1), mix by inverting, and centrifuge at 12000rpm for 10min. Take the supernatant, add chloroform: isoamyl alcohol (24:1), mix by inverting, and centrifuge at 12000rpm for 10min. Pipette the supernatant into a 1.5mL centrifuge tube, add isopropanol, shake up and down, and precipitat...
Embodiment 2
[0078] Example 2 Feasibility identification of the ethnicity of the subject using the relative abundance of Prevotella
[0079] Study population:
[0080] The subjects were male soldiers serving in China. The experimental group consisted of 128 Tibetan male soldiers accustomed to plateaus above 3,500 meters above sea level. The control group consisted of 128 plain Han soldiers who were age-matched with Tibetan soldiers. Strictly control the two groups of people to have the same mixed diet of Chinese troops, maintain the same training environment and training intensity at an altitude of 3500 meters, quit smoking and drinking, and last for 3 months. Soldiers with chronic inflammatory diseases, oral antibiotics, acute infections, and gastrointestinal disorders were excluded.
[0081] method:
[0082] (1) determining the abundance of said microbial marker in said individual's stool sample:
[0083] 1) Collect the feces of the individual to be tested, and extract the DNA of the ...
Embodiment 3
[0099] Example 3 Constructing a ROC curve to compare and evaluate the ability of the relative abundance of bacteria to identify plateau Han population and Tibetan population
[0100] The receiver operating curve (ROC) method was used for verification, and the relative abundance of Prevotella in the feces of the subjects was used to judge its ability to identify the plateau Han population and the Tibetan population. Differences were compared using the Mann-Whitney test because the samples were not normally distributed. The ROC curve was drawn to evaluate the sensitivity and specificity of the relative abundance of the bacterial genera in the differential diagnosis of the plateau Han population and the Tibetan population, and the cutoff value was calculated. P<0.05 was considered statistically significant, and the SPSS22.0 software package was used for statistical processing.
[0101] The result is as figure 2 shown by figure 2 The results showed that the ROC curve showed th...
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