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563 results about "Shake up" patented technology

Kit for detecting pig pseudorabies virus antibodies and block enzyme-linked immuno sorbent assay (ELISA) method

The invention discloses a kit for detecting pig pseudorabies virus antibodies and a block enzyme-linked immuno sorbent assay (ELISA) method. The kit for detecting pig pseudorabies virus antibodies comprises pig pseudorabies virus monoclonal antibodies which are labelled by horseradish peroxidase, wherein the pig pseudorabies virus monoclonal antibodies are monoclonal antibodies obtained by pig pseudorabies viruses as immunogens and the pig pseudorabies viruses are pseudorabies virus strain Ea. The kit for detecting pig pseudorabies virus antibodies also comprises an enzyme label plate, a sample diluent, negative and positive contrasts, a coloured solution, a washing solution, and a stopping solution. The block ELISA method comprises the following steps of 1, taking out a detection plate pre-coated with virus antigens from the kit for detecting pig pseudorabies virus antibodies, adding diluted blood serum needing to be detected into the detection plate pre-coated with the virus antigens, and simultaneously, setting negative and positive contrast apertures, 2, shaking up the diluted blood serum in the negative and the positive contrast apertures, shaking off a solution in the negative and the positive contrast apertures, and washing the detection plate by the washing solution, and 3, adding the pig pseudorabies virus monoclonal antibodies labelled by horseradish peroxidase into the negative and the positive contrast apertures, washing, adding the colored solution into the negative and the positive contrast apertures to carry out room-temperature coloration in the dark, adding the stopping solution into the negative and the positive contrast apertures, and determining OD630nm values of the negative and the positive contrast apertures by an ELISA apparatus. The block ELISA method has the advantages of good singularity, high sensitivity, short detection time, and high accuracy because of utilization of an S/N ratio method in result determination.
Owner:WUHAN KEQIAN BIOLOGY CO LTD

Anti-adhesion plate feeding manipulator with distance detection function

The invention discloses an anti-adhesion plate feeding manipulator with a distance detection function. The anti-adhesion plate feeding manipulator comprises a transverse main shaft. A middle manipulator arm set which extends downwards and is used for sucking plates is arranged in the middle of the transverse main shaft. Shaking manipulator arms extending downwards and air blowing assemblies are correspondingly arranged at the two ends of the transverse main shaft. Each shaking manipulator arm comprises a shaking sucker and a joint connected with the upper end of the shaking sucker. A cavity is formed in each joint. An air inlet hole communicating with the corresponding cavity is formed in one side of each joint. The air inlet holes are connected with a first air source through a first air pipe. The joints are driven by a shaking drive mechanism to shake back and fourth in the vertical direction. The anti-adhesion plate feeding manipulator has the beneficial effects that in the ascending process of the plates, the shaking suckers at the two ends are driven by the shaking drive mechanism to shake up and down, so that the corresponding portions of the top layer of the plate are driven to shake up and down, meanwhile air is blown between the top layer of the plate and the next layer of the plate below the top layer of the plate through nozzles of the blowing assemblies, and plate adhesion is prevented.
Owner:HEFEI UNIV OF TECH +1

Method for determining ammonia nitrogen content in water through Nessler reagent spectrophotometric method

The invention discloses a method for determining ammonia nitrogen content in water through a Nessler reagent spectrophotometric method. The method comprises the following steps: (1) taking an original water sample to be analyzed; (2) performing pretreatment on the original water sample; (3) taking the water sample after pretreatment, loading into a colorimetric tube, shaking up, then adding a Nessler reagent into the colorimetric tube, further shaking up, placing for a certain period of time and adopting a spectrophotometer to test the absorbency of the water sample; (4) adopting distilled water to do a blank test to get blank absorbency; and (5) enabling the absorbency measured in the step (3) to deduct the blank absorbency measured in the step (4) to get correction absorbency, drawing a standard curve and calculating the ammonia nitrogen content in the water. The method has the benefits of fast determining the ammonia nitrogen content in the water, preventing suspended matters and residual chlorine from interfering with the detection result through the pretreatment of the original water sample, and effectively improving the accuracy of the detection result; and furthermore, the method is good in repeatability and less in relative standard deviation.
Owner:SUZHOU GUOHUAN ENVIRONMENT DETECTION

Method for detecting pesticide residues in sulfur-containing vegetables by enzyme inhibition method

InactiveCN102353670AImprove and expand the scope of applicationImprove and extend accuracyMaterial analysis by observing effect on chemical indicatorEnzyme inhibitionPesticide residue
The invention discloses a method for detecting pesticide residues in sulfur-containing vegetables by an enzyme inhibition method. The method comprises the following steps of 1, preparing a standard solution, 2, preparing a sample, 3, testing control groups through the processes of adding a buffer solution into a beaker, adding dropwisely acetate or sodium hydroxide into the beaker, adjusting a pH value of the mixed solution to a pH value of 9, adding an enzyme solution and a color-developing agent into the mixed solution with a pH value of 9, shaking up, standing at a temperature of 37 DEG C for 15 minutes, adding a substrate solution into the mixed solution treated by the previous step, shaking up, and detecting an absorbance variation value delta A0 of the mixed solution undergoing a reaction at wavelength of 410 nanometers for 3 minutes by a spectrometer, 4, adding sulfur-containing vegetable sample extract into a beaker, carrying out processes same as the processes of the step 3, and detecting an absorbance variation value delta A1 of a vegetable sample mixed solution undergoing a reaction at wavelength of 410 nanometers for 3 minutes by a spectrometer, and 5, calculating an enzyme inhibition percentage according to a formula of an enzyme inhibition percentage=[(delta A0-delta A1)/delta A0]*100, wherein when the calculated enzyme inhibition percentage is great than or equal to 50%, it is shown that there are pesticide residues in the detected sulfur-containing vegetable. The method eliminates the interference of sulfides in a sulfur-containing vegetable on pesticide residue detection through adjustment of a pH value of a vegetable sample solution, can rapidly, simply and accurately detect a pesticide residue ratio of a sulfur-containing vegetable, has a low cost, and is suitable for on-site detection.
Owner:TIANJIN UNIV

Establishing method for dendrobe characteristic fingerprint spectrum

ActiveCN105259269ABreak through too vagueClear ingredientsComponent separationAlcoholRetention time
The invention relates to an establishing method for a dendrobe characteristic fingerprint spectrum. The establishing method comprises the following steps: (1) preparing a standard sample liquid: taking dendrobe, smashing, sieving, adding methyl alcohol, ultrasonically filtering, rotating and steaming the filtrate, concentrating till being dry, dissolving with a methyl alcohol solvent, finally keeping constant volume and shaking up to prepare the standard sample liquid; (2) preparing a reference substance liquid: precisely weighting a certain amount of isoschaftoside and diluting with a methyl alcohol solution to prepare the reference substance liquid; (3) measuring a standard fingerprint spectrum: respectively injecting the standard sample solution and the reference substance solution into a high performance liquid chromatograph, and calculating the relative retention time and the retention peak area of each characteristic peak of a standard sample by taking isoschaftoside chromatographic peak as a reference peak of the fingerprint spectrum to prepare the standard fingerprint spectrum. According to the method for establishing the fingerprint spectrum, provided by the invention, the nature of the fingerprint chromatographic peak with the reference peak area being more than 5% is determined, excessive fuzziness of the existing fingerprint spectrum is broken through, the main ingredients of the fingerprint spectrum are further determined and the integrality and ingredient specificity of the dendrobe characteristic fingerprint spectrum are unified.
Owner:北京蓝标一成科技有限公司

Water control type automatic cooking pot

A water control type automatic cooking pot is composed of a stainless steel pot and an electric cooking stove. The pot bottom of the stainless steel pot is made into a cone shape with the middle protruding, a plurality of upper buckle claws are manufactured on the outer circle of the upper end of the stainless steel pot, an air-water isolating air outlet is formed in the left end of a stainless steel pot cover, a sealing ring is fitted on the inner circle of the buckle claw edge of the lower end of the pot cover, a plurality of lower buckle claws are manufactured on the inner circle of the buckle claw edge, a conical electric hot tray is arranged on the electric cooking stove and is matched with the surface of the pot bottom of the stainless steel pot, a temperature sensing probe is assembled in the middle of the electric hot tray, and the upper end face of the temperature sensing probe makes tight contact with the bottom center of the stainless steel pot. Drinking water with different heights is added in the stainless steel pot within the numerical range between the highest position and the lowest position of a water level dividing rule according to different requirements, water addition amount decides the cooking time, the cooked degree of food is decided by the cooking time, and the temperature of the pot bottom can be selected freely between 120 DEG C and 150 DEG C to meet the requirements of juice reducing and the cooked degree of different dishes. When the stainless steel pot is sealed, the stainless steel pot can be shaken up and down by holding a handle to mix fried dish evenly, and the water control type automatic cooking pot can be used for steaming dishes, buns and stuffed buns by adding a food steamer.
Owner:吴文慧 +1

Method for measuring phosphorous content by adopting bismuth phosphomolybdate blue-sulfuric acid photometry

The invention relates to a measurement method of the phosphorous content in an iron ore, in particular to a method for measuring the phosphorous content by adopting a bismuth phosphomolybdate blue-sulfuric acid photometry. The method comprises the following steps of: weighing 0.25g of sample and putting into a platinum crucible containing about 2g of mixed fusing agent, putting in a muffle furnace with the temperature of 100 DEG C, fusing for 10min and taking out; cleaning the exterior of the platinum crucible, then putting the platinum crucible into a beaker containing about 80ml of hot water, adding 320ml of concentrated HNO on an electric furnace, heating at low temperature and dissolving, leaching the crucible out, taking the beaker out, cooling, then transferring into a 250ml volumetric flask, diluting to a scale and shaking up for later use; sucking 25ml of mother solution of a fusing sample in the platinum crucible, putting into a 50ml volumetric flask, adding 2.5ml of bismuth nitrate solution, 5ml of ammonium molybdate solution, 3ml of 8mol/L sulfuric acid solution and 5ml of ascorbic acid solution (prepared when needed), shaking up, carrying out color comparison at 750nm wavelength, measuring an absorbance value, and carrying out result conversion by using a guide sample with similar ingredients. The invention effectively solves the problem of instable absorbance value in a method for measuring the phosphorous content by adopting a traditional molybdenum blue spectrophotometry and ensures that the absorbance remains unchanged within two hours.
Owner:迁安市津唐球墨铸管有限公司

Vein blood sample shaking up device for clinical laboratory of hospital

The invention discloses a vein blood sample shaking up device for a clinical laboratory of a hospital. The vein blood sample shaking up device comprises a base, a guide rod, a screw rod, a hoisting block, an arc-shaped clamping claw, a shaking up platform, a rectangular translation frame, a driving motor, an incomplete gear and a fixed sleeve ring, and is characterized in that the hoisting block is mounted on the screw rod through a thread; a clamping force arm is fixedly mounted at the right end of a shaft sleeve through a connecting rod; gears are arranged at front and rear sides in the rectangular translation frame; the gears in the rectangular translation frame are adaptive to a gear on the incomplete gear; the fixed sleeve ring is fixedly connected to the rear side of the rectangular translation frame trough a connecting rod. According to the vein blood sample shaking up device disclosed by the invention, a test tube is indirectly driven by manually rotating a shaking up wheel to move downward into the fixed sleeve ring, so that the shaking up of blood collection tubes with different lengths is met and the commonality of the shaking up device is improved; the incomplete gear is matched with the rectangular translation frame so that the fixed sleeve ring moves leftward and rightward and the blood collection tubes are driven to swing leftward and rightward, and furthermore, the shaking up effect of vein blood is improved.
Owner:袁润栋

Method for synthesizing water soluble nanometer iron through mangosteen pericarp in environment-friendly mode and application of water soluble nanometer iron

The invention discloses a method for synthesizing water soluble nanometer iron through mangosteen pericarp in an environment-friendly mode and application of the water soluble nanometer iron, and belongs to the technical field of treatment of waste water. The method for preparing water soluble nanometer iron includes the steps that first, the mangosteen pericarp is washed with distilled water, dried, crushed and screened to 300 microns to 600 microns, and after the mangosteen pericarp particles and distilled water are mixed, heated to boiling and cooled to the room temperature, mangosteen pericarp extraction solution is obtained through filtration; second, under the condition of the room temperature, the mangosteen pericarp extraction solution and ferric salt solution are mixed according to a certain proportion, oscillated and shaken up, and then the water soluble nanometer iron is obtained. The water soluble nanometer iron prepared with the method can be used for degrading reactive brilliant blue KN-R and reactive brilliant red K-2G dye in a catalytic mode, and the degrading efficiency is high. The method for environmentally friendly synthesizing water soluble nanometer iron through mangosteen pericarp is simple in technology and low in cost, waste is treated with waste, the utilization of mangosteen pericarp resources is intensified, and dye in the waste water is also removed.
Owner:BEIJING UNIV OF TECH

Acid milk beverage with suspended fruit granules and preparation process method

The invention relates to an acid milk beverage with suspended fruit granules and a preparation process method, and the preparation process method comprises the following steps: firstly, preparing a suspension stabilizer for preparing the acid milk beverage with the suspended fruit granules, then uniformly dry-mixing the suspension stabilizer with white granulated sugar and preparing for yoghurt; then uniformly dissolving other accessories except the fruit granules and citric acid in water, adding into the yoghurt with the suspension stabilizer, and uniformly stirring; then loading the fruit granules into a finished product packaging container, further filling and adding the yoghurt with the suspension stabilizer, sealing, and sterilizing; and shaking up after cooling completely, thus obtaining a finished product. By utilizing the suspension stabilizer, not only the cost can be saved, but also the good stability, the water retention property and the excellent suspension performance canbe realized in the acid milk beverage, and the fruit granules can be uniformly suspended in the acid milk beverage; within 9 months at normal temperature, the fruit granules can be uniformly suspended without sedimentation, the layer separation of milk can be avoided, the water separation can be avoided, and the stable uniform state can be maintained.
Owner:DONG GUAN SHI LONG JIN WEI BEVERAGE & FOOD

Method for detecting residual quantity of 18 sulfanilamide drugs in beef and mutton

The invention discloses a method for detecting residual quantity of 18 sulfanilamide drugs in beef and mutton. The method comprises taking 5g of uniform beef and mutton, adding anhydrous magnesium sulfate, sodium acetate and acetonitrile acetate solutions into the beef and mutton, carrying out high-speed homogenized extraction, carrying out centrifugation, carrying out vortex mixing purification impurity-removal on the supernatant by an adsorbent containing a plurality of purification materials, carrying out pressure reduction or nitrogen blowing condensation on the supernatant subjected to re-centrifugation until drying, redissolving the residues, after shaking-up, filtering the solution by a microfiltration membrane with pore sizes of 0.22 micrometers to obtain a sample solution, carrying out separation by a reversed phase column filled with C18 as a filler, carrying out ultraviolet light on-line rapid derivation, carrying out detection by a fluorescence detector, carrying out qualitative analysis according chromatographic peak retention time, carrying out quantification by an external standard method and calculating residual quantity of 18 sulfanilamide drugs in beef and mutton. The method has simple processes, utilizes less amount of a reagent, has high detection sensitivity, releases simultaneous rapid detection of 18 sulfanilamide drugs by one step, greatly shortens analysis time and has a good application prospect.
Owner:CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST

Method for quickly measuring silicon dioxide and modulus in potash water glass

The invention relates to the field of detecting potash water glass, in particular to a method for quickly measuring the potash water glass for fluorescent powder. The method comprises the following steps of: 1) accurately weighing the potash water glass, and shaking up the potash water glass uniformly to obtain solution of the potash water glass; 2) adding a methyl red indicator into the solution; 3) titrating K2O by using solution of hydrochloric acid until the K2O turns red from yellow, and measuring the content of the K2O, wherein the solution is also used for measuring SiO2; 4) adding sodium fluoride into the solution, namely adding the sodium fluoride into the solution after measuring the content of the K2O, and dissolving the sodium fluoride to ensure that the solution turns yellow; 5) titrating silicon dioxide, namely adding standard solution of the hydrochloric acid when the solution turns red; 6) titrating the excessive hydrochloric acid by using the standard solution of sodium hydroxide until the solution turns yellow; and 7) performing a blank experiment, namely adding the standard solution of the hydrochloric acid, and performing the titration slowly by using the standard solution of the sodium hydroxide until the solution turns yellow. The method can quickly and accurately measure the silicon dioxide and the modulus in the potash water glass for the fluorescent powder.
Owner:CAIHONG GRP ELECTRONICS CO LTD

Green juglans regia high-efficiency washing device

The invention discloses a green juglans regia high-efficiency washing device. The green juglans regia high-efficiency washing device comprises a bottom plate, wherein a left vertical plate and a rightvertical plate are vertically and fixedly mounted on the bottom plate; top plates are respectively, horizontally and fixedly mounted at the top of the left vertical plate and the top of the right vertical plate; a rotating wheel is arranged on the top plates; a left gear which is in meshing connection with a left rack is arranged on the left side of the left rack, and a right gear which is in meshing connection with a right rack is arranged on the right side of the right rack; each pressing plate is rotatably connected to a corresponding extending plate; and scraping hairs propped to the sidewall of a lifting basket are arranged on the right side wall of the pressing plate. According to the green juglans regia high-efficiency washing device disclosed by the invention, through the rotation of a brush disk, green juglans regia is washed rotatably; when the brush disk moves downwards, the lifting basket moves upwards, so that the pressing force of the brush disk to the green juglans regia is strengthened, and the washing effect is further improved; the washing stability is improved by the deformation of a damping spring; besides, the green juglans regia in the lifting basket can shake up and down by the up-and-down reciprocating movement of the lifting basket, so that sundries on the surface of the green juglans regia can be quickly separated, and the washing effect and the washing speed of the green juglans regia are greatly improved.
Owner:胡立鸣

Method for measuring copper content in tin-silver-copper solder through iodometry

Provided is a method for measuring copper content in tin-silver-copper solder through iodometry. The method comprises the following steps: a copper standard solution is prepared and the titer of the copper standard solution is measured. A sample to be measured is weighed and added into an Erlenmeyer flask. Concentrated sulfuric acid is added and the mixture is heated for dissolution. The above solution is cooled to the room temperature, perchloric acid is added, after the sample is dissolved fully, heating is carried out until white smoke is generated, concentrated hydrochloric acid is dropwise added into the above solution in batches to remove the tin element in the solution, and the solution is subjected to concentration. The Erlenmeyer flask is taken down and cooled to the room temperature, deionized water is added, the constant volume is 100mL and the above solution is shaken up. Then an ammonium hydroxide solution is added and copper ammonia complex ions are formed. Then ammonium bifluoride is added into the solution and stirred until the blue color disappears. The above solution is cooled to the room temperature by utilization of running water. The solution is placed for half a minute, then potassium iodide is added, and immediately the solution is subjected to titration by a sodium hyposulfite standard solution until a shallow yellow color appears. Then a potassium thiocyanate solution and a starch solution are added, and the solution is subjected to titration by the sodium hyposulfite standard solution until a blue color disappears. The volume of the consumed sodium hyposulfite solution is recorded, and the content of copper in the sample is calculated.
Owner:BEIJING INST OF NONFERROUS METALS & RARE EARTH

Valerian preparation and preparation method thereof

The invention discloses valerian preparation, which consists of valerian volatile oil, beta-cyclodextrin, valerian extract powder, starch for pharmaceutical, ethanol, magnesium stearate and talcum powder. A preparation method comprises the steps: A. adopting fresh valerian rhizomes, washing off mud and directly performing distillation through water-jacket pressurized steam after draining the valerian rhizomes to obtain the volatile oil and decoction dregs, B. drying the decoction dregs, placing the decoction dregs, using fresh medicinal materials to percolate the decoction dregs, filtering percolation liquid, recovering ethanol and then performing concentration to obtain the valerian extract powder, C. adding beta-cyclodextrin of the volatile oil into distilled water to be shaken up, then adding the valerian volatile oil to perform lapping and inclusion, using petroleum ether to wash off volatile oil which is not included and performing drying to obtain beta-cyclodextrin inclusion powder of the valerian volatile oil, and D. mixing the beta-cyclodextrin inclusion powder of the valerian volatile oil and the valerian extract powder to be filled into capsules. The valerian preparation is safe, effective, controllable and stable, has remarkable effects of resolving tetany, relieving pain and tranquilizing and soothing the nerves on digestive tract smooth muscles and can be used for treating smooth muscle spasmodic pain.
Owner:武汉联合药业有限责任公司

Preparation method for chrysanthemum synthetic nanometer zero-valent iron suspension liquid

The invention discloses a preparation method for chrysanthemum synthetic nanometer zero-valent iron suspension liquid. The preparation method comprises the following steps of (1) soaking dry chrysanthemum in water at the temperature of 40-90 DEG C for 10-180 minutes under the condition that the proportion of the chrysanthemum to the water is 5-25 grams to 1 liter, and adding alcohol in the mixture of the chrysanthemum and the water under the condition that the proportion of the water and the alcohol is 1: (0.001-0.1) so as to obtain primary chrysanthemum liquor; (2) primarily filtering the chrysanthemum liquor by using a sieve of which the meshes are not lower than 200; (3) performing vacuum filtering on the chrysanthemum liquor by a sieve with the meshes not smaller than 0.49 micrometer so as to obtain a chrysanthemum extract; and (4) adding ferric salt water liquor with the molar concentration of 0.30-0.60M in the chrysanthemum extract under the condition that the volume ratio is 1: (0.1-1), mixing the ferric salt water liquor and the chrysanthemum extract, and shaking up to obtain the zero-valent iron suspension liquid. The preparation method is economical and environment-friendly; the cost of a product is low; and the prepared suspension liquid is high in quality and high in stability.
Owner:李广 +1
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