22 results about "Microbial Genes" patented technology

A microbial gene editing scheme automatic design method, system and storage medium; the method comprises: selecting a design object for different types of strains, the design object is bacteria or fungi; after determining the target gene, obtaining the target gene information from the NCBI database; the design scheme of bacteria comprises the following steps: determining the gRNA design region; selecting the optimal gRNA sequence; doing sequence complexity and GC content analysis; the design scheme of fungi uses a frameshift scheme: the gRNA of the frameshift scheme is set at any position not overlapping with other genes after the start codon in the gene; select gRNA sequence in gRNA design region, select the optimal gRNA sequence; design two pairs of four primer sequences for homologous arm identification primer; this scheme can replace the complicated and time-consuming manual scheme, save a lot of time, solve the problem of high cost in the design of the best site and the design of sgRNA sequence scheme of artificial gene knockout.

This invention relates to the fields of microbial genetic engineering and fermentation biomanufacturing technology, specifically disclosing a recombinant Corynebacterium glutamicum with high 5-aminolevulinic acid (ALA) production and its applications. Addressing the problems of existing strains such as crude enzyme activity regulation, inhibited cell growth, the need for external inhibitors, and low yield, this invention uses Corynebacterium glutamicum as a host and removes the gdhA gene without scarring to reduce precursor competitive metabolism. Site-directed mutations of the endogenous hemB gene (S288A, D128E, R231K) are performed to moderately reduce dehydratase activity. A high-expression plasmid pXMJ19-hemA containing the hemA gene from Rhodopsinus capsulatum is constructed and introduced into the modified strain to obtain a recombinant strain with optimized triple metabolism. This strain reduces product degradation without the need for external dehydratase inhibitors, does not affect cell growth, and achieves a yield up to 4.30 times higher than the control after 42 hours of fermentation, with ALA accumulation exceeding 8.9 g / L. The strain exhibits stable characteristics, the process is simple, and it has good industrial application value.

This invention discloses a method for preparing self-healing concrete based on microbial gene programming, belonging to the field of building materials technology. The method includes: constructing a recombinant engineered strain incorporating a urease gene cluster and a carbonic anhydrase gene; preparing a spore suspension and protecting it with a three-layer microencapsulation system of sodium alginate / chitosan / gelatin; preparing nutrient-releasing microspheres encapsulating calcium lactate and urea in a polylactic acid-glycolic acid copolymer; and mixing cement, standard sand, water, fly ash, microencapsulated spore particles, nutrient-releasing microspheres, nano-silica, and modified bentonite to prepare self-healing concrete. This invention enhances the urease activity of the strain through genetic engineering, protects the spores using a three-layer microencapsulation technology, and combines a nutrient-releasing system and nano-synergists to achieve efficient self-repair of concrete cracks.

This invention discloses a high-yield lactyl-3-4 ...5-4-4-4-4-4- N This invention relates to plasmid-free genetically engineered strains of tetrasaccharides and their applications, belonging to the field of microbial genetic engineering. The invention screened strains with higher activity. β -1,3-galactosyltransferase gene PmgalT Computer-aided enzyme function modification was used to achieve a shake-flask yield of 10.05 g / L for the constructed mutant. Subsequently, genome integration was performed... breast Multi-copy integration PmgalT * V27I / V39I / Y147F ‑galE‑galU A plasmid-free strain was constructed, enabling it to grow in shake flasks with lactoyl- N The yield of tetrasaccharides reached 14.4 g / L. Using glucose as the sole carbon source, the highest yield of 68.19 g / L was achieved at 64.5 h; using glycerol as the sole carbon source, the highest yield of 62.88 g / L was achieved at 60.5 h, providing a basis for its industrial production.

This invention relates to the fields of microbial genetic engineering and brewing technology, and provides a PDC1 gene mutant strain resistant to 2-phenylethanol and its application in the brewing of wheat-aroma baijiu. The mutant strain is *Saccharomyces cerevisiae* PDC1_473 (HM 03), with the preservation number CCTCC M20252996. The PDC1 gene of this strain has a base mutation from T to C at position 473. When this mutant strain is applied to the solid-state fermentation of wheat-aroma baijiu, it becomes the absolutely dominant fungal community, significantly altering the microbial community structure of the fermentation system, inhibiting the growth of various conditionally pathogenic and spoilage bacteria, strengthening the fermentation environment centered on *Lactobacillus*, and significantly enhancing the rose, citrus, grassy, ​​fatty, and caramel aromas of the baijiu. It imparts a pleasant, rich ester aroma and complex aroma to the baijiu, increasing its fullness, and has broad application potential in the brewing of wheat-aroma baijiu.

Genetically engineered bacteria which express RNAs or proteins that produce ammonia upon decreases in phosphate concentrations are disclosed.

This invention discloses a porcine CD40L trimer protein, a recombinant Bacillus subtilis strain expressing this protein, and its applications, belonging to the fields of microbial genetic engineering and livestock and poultry biological products technology. This invention utilizes Bacillus subtilis WB800N as a host and pHT43 as a vector to successfully construct a recombinant strain that efficiently secretes and expresses porcine CD40L trimer protein. Soluble expression of the porcine CD40L trimer protein was achieved. The amino acid sequence of the porcine CD40L trimer protein expressed by the recombinant strain is shown in SEQ ID NO:1. Immunological activity verification using this porcine CD40L trimer protein revealed that the CD40L trimer protein expressed in this invention can simultaneously activate humoral and cellular immunity, possessing practical application value as a novel vaccine adjuvant.

The invention relates to the technical field of precision agriculture, in particular to a scientific fertilization method based on detection of soil conditions and microbial action, which comprises the following steps: collecting soil impedance and microbial indexes, correcting microbial distribution by using impedance depth, and executing environmental inhibition attenuation on nitrogen fixation and phosphorus solubilization rate by combining a carbon source and acid-base buffer capacity. And quantifying biological available nutrient supply, and generating a fertilizer discharge signal based on a crop demand gap and mechanical parameters. According to the method, the vertical cone index and the microbial gene abundance are collected, physical impedance data are introduced to correct the microbial spatial distribution weight, the metabolic activation model is constructed in combination with active organic carbon and the acid-base buffer capacity, and the actual nutrient conversion efficiency and the effective fertilizer supply total amount of nitrogen-fixing bacteria and phosphate solubilizing bacteria are quantified; biological fertilizer supply capacity is brought into a nutrient income and expenditure balance system, a discrete fertilizer discharge control instruction is generated according to actual gaps of crops and mechanical operation parameters, and the utilization rate of chemical fertilizer and agricultural ecological benefits are improved.

This invention relates to the field of microbial genetic engineering technology, specifically to a recombinant vector for enhanced oil recovery (EOR), engineered bacterial strains for EOR, their construction methods and applications, engineered bacterial strain agents and their applications, and methods for crude oil recovery. The recombinant vector for EOR of this invention includes a starting vector and an expression gene integrated into the starting vector; the expression gene includes a surfactant synthesis gene and a heat-resistant gene; the surfactant synthesis gene is the srfA gene; the heat-resistant gene is selected from the hsp70 gene, hsp60 gene, or dnaK gene. In this invention, a dual-gene synergistic modification strategy is employed, simultaneously introducing a heat-resistant gene and a surfactant synthesis gene and optimizing their expression, thereby achieving a dual enhancement of the engineered strain's environmental adaptability and metabolic function.

Provided is a promoter, which has a nucleotide sequence shown in SEQ ID NO. 1. The promoter is obtained by means of rationally designing the promoter of a dapA gene, has obviously reduced transcriptional activity and can be used for the weakened expression of microorganism genes. Substituting the original promoters of the dapA gene, a ddh gene, an ilvA gene, a tdcB gene and a gltA gene in Corynebacterium glutamicum by using the promoter enables the expression levels of these genes to be significantly reduced, the carbon metabolic in a recombinant microorganism constructed thereby flows more to a threonine anabolism pathway, and the recombinant microorganism has a significantly improved production of threonine in comparison with an original strain and has good growth performance.

The application discloses a method for optimizing protein translation extension process to improve protein yield of saccharomyces cerevisiae, and belongs to the technical field of microbial genetic engineering. The application improves the global protein synthesis capacity of saccharomyces cerevisiae by regulating the translation elongation factor in the translation initiation process, so that the protein content of saccharomyces cerevisiae is increased from 45.63 g / 100 g to 48.06 g / 100 g, and the protein yield is increased from 3.23 g / L to 3.46 g / L. The application provides technical support for low-cost and green single-cell protein production, and has a wide application prospect in the field of protein substitution.

The present invention relates to compositions and methods for preventing or treating diseases or disorders involving nucleic acid damage, such as cancer, in a subject, as well as methods of identifying gut microbes, strains or metabolites thereof associated with nucleic acid damage and diseases or disorders involving nucleic acid damage, such as cancer.

This invention discloses a Bacillus bellis strain overexpressing a lipopeptide synthase gene, its construction method, and its application in fruit preservation. It belongs to the fields of microbial genetic engineering and postharvest fruit preservation technology. Using Bacillus bellis QB250122 as the starting strain, two rounds of genome editing were performed using a dual-plasmid temperature-sensitive CRISPR-Cas9n system. In the first round, the srfA promoter was replaced with the PHpaII superpromoter. In the second round, a complete expression cassette was inserted at the bacA site. The engineered strain produced 900-1200 mg / L of surfactantin. Applying a bacterial suspension to the surface of fruits such as mangoes can extend shelf life by 10-15 days.

The present application relates to the technical field of data processing, in particular to a rice field mulching degradation condition carbon emission prediction method based on multi-source data fusion. The present application obtains the data of rice field environment, mulching physicochemical and microbial gene set; adopts incremental principal component analysis to extract target principal component, and constructs non-biodegradation time-varying characteristic matrix; the standardized path coefficient is calculated by using structural equation model and is converted into graph convolution network topology adjacency matrix, and the foregoing characteristic matrix is input into the network to obtain the micro-ecological causal network characteristic matrix; the two matrices are subjected to bilinear tensor pooling operation, and a high-dimensional fusion tensor representing the synergistic effect is generated; finally, it is input into the reinforcement learning network as the environmental state, and the strategy optimization is carried out combined with the physicochemical constraint reward function and the predicted flux is output. The present application solves the problems of feature extraction shallow and prediction black box, and significantly improves the accuracy and mechanism explanation ability of greenhouse gas emission prediction.

This invention belongs to the field of microbial genetic engineering and metabolic engineering technology, specifically relating to decarboxylase mutants in the indolepyruvate pathway, their mutant combinations, and their application in the production of indoleacetic acid. A decarboxylase mutant was obtained by mutating glycine at position 255 of the indolepyruvate decarboxylase kivD derived from *Lactococcus lactis* to tyrosine, and serine at position 386 to glutamic acid. kivD G255Y / S386E The applicant expressed kivD freely in the engineered recombinant Bacillus licheniformis strain BLI-2. G255Y / S386E The strain BlI-3 was obtained, and the indoleacetic acid (IAA) yield of BlI-3 reached 675.6 mg / L, which was 70.09% higher than that of BlI-2 and 258.44% higher than that of BlI-1, significantly improving the level of IAA synthesis by the host strain.

The present application belongs to the field of microbial genetic engineering technology, and particularly relates to tumor-targeting drug-producing engineering strains and a construction method and application thereof. The present application discloses a series of tumor-targeting drug-producing engineering strains, which can produce the anticancer drug romidepsin in situ in a tumor, can heterologously express romidepsin, and can produce romidepsin in situ in a tumor of a tumor-bearing small animal and has the activity of inhibiting tumor growth. That is, the present application provides a series of Escherichia coli Nissle 1917 recombinant strains, which are expected to become an anticancer live bacterial drug and will be applied to the clinic in the future, and therefore have good practical application value.

The application provides a primer composition, a kit and application thereof for efficiently detecting common pathogenic bacteria of human, relates to the technical field of microorganism gene detection, and aims at solving the defects of low detection efficiency, poor specificity, low sensitivity of traditional PCR technology, and non-specific amplification of traditional LAMP detection technology. The application designs primers according to known sequences of six common pathogenic bacteria of human, i.e., Candida albicans, Chlamydia trachomatis, Escherichia coli, group B streptococcus, Mycoplasma hominis and Streptococcus pneumoniae, and provides a kit with a micro-fluidic chip as a carrier and application thereof, which are used for detecting common pathogenic bacteria of human, have high specificity and high sensitivity, reduce detection cost and detection time, and can realize on-site rapid detection of common pathogenic bacteria of human.

This invention provides a genome editing method for *Yersinia lipolytica* based on homologous recombination, belonging to the fields of microbial genetic engineering and synthetic biology. This invention constructs the tool plasmid pUrloxP3 to achieve efficient knockout and combined knockout of ylAS and ylPDH, completing site-specific integration and functional verification of exogenous genes RgTAL, 4CL, and CHS. The Cre / loxP system is used to recover the URA3 marker and eliminate the pYLXP'-Cre helper plasmid. This invention features high editing efficiency, standardized procedures, cyclical selection markers, and stable integration of exogenous genes, ultimately yielding a stable engineered strain without markers or helper plasmids. It is suitable for multi-round metabolic engineering and cell factory construction of *Yersinia lipolytica*.

This invention discloses a *Stachys pubescens* alcohol dehydrogenase, its encoding gene, and its applications, relating to the field of microbial genetic engineering technology. The alcohol dehydrogenase is any one of (1), (2), and (3): (1) the SvADH1 protein, whose amino acid sequence is shown in SEQ ID NO.3; (2) a protein obtained by substituting, deleting, and / or adding one or more amino acid residues to the amino acid sequence of the SvADH1 protein, resulting in a protein with more than 70% identity to the SvADH1 protein and associated with the redox of cross-linked solanol A; (3) a fusion protein obtained by attaching a protein tag to the N-terminus and / or C-terminus of (1) or (2). This invention has found that overexpression of the encoding gene of this alcohol dehydrogenase in *Stachys pubescens* significantly increases the yield of cross-linked solanol A, thus providing a new direction for the development of microbially derived cross-linked solanol A.

The application discloses a genetically engineered Gujeromyces reessii and a method for producing beta-glucan, and relates to the technical field of microbial genetic engineering. On the basis of wild-type Gujeromyces reessii, at least one of the following genes is overexpressed: beta-glucan synthase gene GSC1, FKS1 gene and RHO1 gene. The yield of beta-glucan produced by the genetically engineered Gujeromyces reessii obtained based on the above gene engineering modification can be increased by more than 30% compared with that of the wild-type strain. The harvested beta-glucan has good activity and can be directly used for high-end functional food, immune adjuvant, wound dressing and high-grade cosmetic raw materials; and the application provides a novel and efficient cell factory for green manufacturing of beta-glucan.

Provided herein is the first commensal microbial gene encoding an enzyme that catalyzes the conversion of androstenedione to epitestosterone in the gut bacterium Clostridium scindens (desF). Also provided is an important and unrecognized capability for epitestosterone in androgen receptor-dependent prostate cancer cell proliferation and the potential clinical relevance of this bacterial enzymatic pathway (desF) in prostate cancer. Additionally, it was discovered that bacterial isolates from urine or prostatectomy tissue are capable of androgen production and that urinary tract bacteria can exhibit 17β-hydroxysteroid dehydrogenase activity The gene in urinary isolates encoding 17β-hydroxysteroid dehydrogenase that catalyzes the conversion of androstenedione to testosterone was identified and named desG.

The application discloses a methane seepage microbial marker mining method based on multi-source data fusion and related equipment, and the embodiment of the application fuses microbial gene data, spatial information and environmental geochemical data, constructs a multi-dimensional feature space, and improves the data dimension and reliability of marker mining; moreover, the embodiment of the application adopts a combination of random forest and regression modeling, realizes quantitative screening of the marker, and reduces manual intervention and subjective bias; specifically, the embodiment of the application grades the marker based on the comprehensive score of the three dimensions of importance, stability and universality, facilitating priority selection in subsequent practical applications; the embodiment of the application is suitable for methane seepage identification in different sea areas and different geological backgrounds, has good generalizability and practicality, and can be widely applied in the technical field of marker analysis.