73 results about "Microbial Genes" patented technology

The invention belongs to the technical field of microbial genetic engineering, and particularly relates to an alpha-2, 3-sialyltransferase mutant and application thereof. The a-2, 3-sialyltransferase mutants V175N, P198A, G173D, D174Q, F196K and A315H are obtained by performing site-specific mutagenesis on six amino acids in an a-2, 3-sialyltransferase amino acid sequence coded by a gene derived from Campylobacter jejuni (OH4384), the yield of 3 '-sialyllactose is remarkably increased by single-point mutagenesis of the V175N, the yield of 3'-sialyllactose is remarkably increased by single-point mutagenesis of the V175N, the yield of 3 '-sialyllactose is remarkably increased by single-point mutagenesis of the P198A, the G173D, the D174Q, the F196K and the A315H, the optimal recombinant microbial strain 3 '-SL-09 (V175N / P198A / D174Q) is obtained through combination of multiple mutation sites, the yield can reach 5.91 g / L, the bottleneck problem of low catalytic efficiency of sialyltransferase is effectively solved, and an enzyme element with excellent performance is provided for high-efficiency and low-cost biological manufacturing of 3'-SL.

The invention discloses a methane leakage microbial marker mining method based on multi-source data fusion and related equipment, according to the embodiment of the invention, a multi-dimensional feature space is constructed by fusing microbial gene data, spatial information and environmental geochemical data, and the data dimension and reliability of marker mining are improved; moreover, according to the embodiment of the invention, a mode of combining random forest and regression modeling is adopted, so that quantitative screening of the markers is realized, and manual intervention and subjective deviation are reduced; specifically, according to the embodiment of the invention, the markers are graded on the basis of comprehensive scores of three dimensions of importance, stability and universality, so that priority selection in subsequent practical application is facilitated; the embodiment of the invention is suitable for methane leakage identification of different sea areas and different geological backgrounds, has good generalizability and practicability, and can be widely applied to the technical field of marker analysis.

A microbial gene editing scheme automatic design method, system and storage medium; the method comprises: selecting a design object for different types of strains, the design object is bacteria or fungi; after determining the target gene, obtaining the target gene information from the NCBI database; the design scheme of bacteria comprises the following steps: determining the gRNA design region; selecting the optimal gRNA sequence; doing sequence complexity and GC content analysis; the design scheme of fungi uses a frameshift scheme: the gRNA of the frameshift scheme is set at any position not overlapping with other genes after the start codon in the gene; select gRNA sequence in gRNA design region, select the optimal gRNA sequence; design two pairs of four primer sequences for homologous arm identification primer; this scheme can replace the complicated and time-consuming manual scheme, save a lot of time, solve the problem of high cost in the design of the best site and the design of sgRNA sequence scheme of artificial gene knockout.

This invention relates to the fields of microbial genetic engineering and fermentation biomanufacturing technology, specifically disclosing a recombinant Corynebacterium glutamicum with high 5-aminolevulinic acid (ALA) production and its applications. Addressing the problems of existing strains such as crude enzyme activity regulation, inhibited cell growth, the need for external inhibitors, and low yield, this invention uses Corynebacterium glutamicum as a host and removes the gdhA gene without scarring to reduce precursor competitive metabolism. Site-directed mutations of the endogenous hemB gene (S288A, D128E, R231K) are performed to moderately reduce dehydratase activity. A high-expression plasmid pXMJ19-hemA containing the hemA gene from Rhodopsinus capsulatum is constructed and introduced into the modified strain to obtain a recombinant strain with optimized triple metabolism. This strain reduces product degradation without the need for external dehydratase inhibitors, does not affect cell growth, and achieves a yield up to 4.30 times higher than the control after 42 hours of fermentation, with ALA accumulation exceeding 8.9 g / L. The strain exhibits stable characteristics, the process is simple, and it has good industrial application value.

The invention belongs to the technical field of microbial genetic engineering, and particularly relates to an acid-resistant lactobacillus plantarum engineering bacterium as well as a construction method and application thereof. The recombinant lactobacillus plantarum WCFS1-gadA-speA is obtained by constructing a recombinant expression vector pMG36e-gadA-speA and inducing the expression of two key genes, and the recombinant lactobacillus plantarum WCFS1-gadA-speA is obtained. The analysis of the fermentation capacity of the strain finds that compared with a single gene heterologous expression strain, the recombinant lactobacillus plantarum WCFS1-gadA-speA shows higher acid resistance and apple juice fermentation capacity. The recombinant lactobacillus plantarum WCFS1-gadA-speA with higher strong acid tolerance is obtained through a heterologous expression technology, a strain resource with better performance is provided for the food fermentation industry, and the quality and stability of a fermented product are improved.

This invention belongs to the field of microbial genetic engineering technology, specifically relating to an α-2,6-sialic acid transferase mutant and its application. This invention utilizes a mutant derived from *Lithocarpus lucida* (…). Photobacterium Five amino acids in the amino acid sequence of α-2,6-sialyl transferase encoded by the gene of sp. JT-ISH-224 were mutated at specific sites to obtain α-2,6-sialyl transferase mutants M133L, E140D, D228H, N289D, and G322Q. Among them, the E140D and G322Q single-site mutants of α-2,6-sialyl transferase showed increases in 6'-SL yield of 87.80% and 78.01%, respectively, compared with wild-type α-2,6-sialyl transferase. The optimal recombinant microbial strain 6'-SL-7 (E140D / G322Q) obtained by combining the mutation sites achieved a yield of 1.471 g / L, effectively solving the bottleneck problem of low catalytic efficiency of sialyl transferase and providing a high-performance enzyme element for the efficient and low-cost biomanufacturing of 6'-SL.

This invention discloses a method for preparing self-healing concrete based on microbial gene programming, belonging to the field of building materials technology. The method includes: constructing a recombinant engineered strain incorporating a urease gene cluster and a carbonic anhydrase gene; preparing a spore suspension and protecting it with a three-layer microencapsulation system of sodium alginate / chitosan / gelatin; preparing nutrient-releasing microspheres encapsulating calcium lactate and urea in a polylactic acid-glycolic acid copolymer; and mixing cement, standard sand, water, fly ash, microencapsulated spore particles, nutrient-releasing microspheres, nano-silica, and modified bentonite to prepare self-healing concrete. This invention enhances the urease activity of the strain through genetic engineering, protects the spores using a three-layer microencapsulation technology, and combines a nutrient-releasing system and nano-synergists to achieve efficient self-repair of concrete cracks.

This invention discloses a high-yield lactyl-3-4 ...5-4-4-4-4-4- N This invention relates to plasmid-free genetically engineered strains of tetrasaccharides and their applications, belonging to the field of microbial genetic engineering. The invention screened strains with higher activity. β -1,3-galactosyltransferase gene PmgalT Computer-aided enzyme function modification was used to achieve a shake-flask yield of 10.05 g / L for the constructed mutant. Subsequently, genome integration was performed... breast Multi-copy integration PmgalT * V27I / V39I / Y147F ‑galE‑galU A plasmid-free strain was constructed, enabling it to grow in shake flasks with lactoyl- N The yield of tetrasaccharides reached 14.4 g / L. Using glucose as the sole carbon source, the highest yield of 68.19 g / L was achieved at 64.5 h; using glycerol as the sole carbon source, the highest yield of 62.88 g / L was achieved at 60.5 h, providing a basis for its industrial production.

The application belongs to the technical field of kidney disease differential diagnosis, and particularly relates to a microbial gene marker for differentiating diabetic nephropathy and membranous nephropathy and application thereof. The application provides sufficient evidence for the differentiation and treatment of clinical diagnosis of diabetic nephropathy and pathological diagnosis of membranous nephropathy based on four microbial markers of Blautia, Akkermansia, Sphingomonas and Granulicatella.

The invention relates to an underground water nitrogen metabolism function prediction and regulation method based on a three-dimensional fluorescence spectrum and automatic machine learning. The method comprises the following steps: collecting underground water microbial gene sequencing serial numbers in literatures and fluorescence emission spectrum data of corresponding water samples; according to the obtained gene sequencing data serial number, downloading microbial gene sequencing data, and carrying out normalization processing on required emission spectrum data; selecting a functional gene reflecting underground water nitrogen metabolism capability and calculating relative abundance; and constructing a machine learning model of underground water nitrogen metabolism. The selected optimal model can accurately predict and understand underground water nitrogen metabolism, reveal the influence of DOM fluorescence properties on an underground water ecosystem, and can be used for predicting the underground water microorganism nitrogen metabolism function of an unknown area.

This invention relates to the fields of microbial genetic engineering and brewing technology, and provides a PDC1 gene mutant strain resistant to 2-phenylethanol and its application in the brewing of wheat-aroma baijiu. The mutant strain is *Saccharomyces cerevisiae* PDC1_473 (HM 03), with the preservation number CCTCC M20252996. The PDC1 gene of this strain has a base mutation from T to C at position 473. When this mutant strain is applied to the solid-state fermentation of wheat-aroma baijiu, it becomes the absolutely dominant fungal community, significantly altering the microbial community structure of the fermentation system, inhibiting the growth of various conditionally pathogenic and spoilage bacteria, strengthening the fermentation environment centered on *Lactobacillus*, and significantly enhancing the rose, citrus, grassy, ​​fatty, and caramel aromas of the baijiu. It imparts a pleasant, rich ester aroma and complex aroma to the baijiu, increasing its fullness, and has broad application potential in the brewing of wheat-aroma baijiu.

Genetically engineered bacteria which express RNAs or proteins that produce ammonia upon decreases in phosphate concentrations are disclosed.

This invention discloses a porcine CD40L trimer protein, a recombinant Bacillus subtilis strain expressing this protein, and its applications, belonging to the fields of microbial genetic engineering and livestock and poultry biological products technology. This invention utilizes Bacillus subtilis WB800N as a host and pHT43 as a vector to successfully construct a recombinant strain that efficiently secretes and expresses porcine CD40L trimer protein. Soluble expression of the porcine CD40L trimer protein was achieved. The amino acid sequence of the porcine CD40L trimer protein expressed by the recombinant strain is shown in SEQ ID NO:1. Immunological activity verification using this porcine CD40L trimer protein revealed that the CD40L trimer protein expressed in this invention can simultaneously activate humoral and cellular immunity, possessing practical application value as a novel vaccine adjuvant.

The invention relates to the technical field of precision agriculture, in particular to a scientific fertilization method based on detection of soil conditions and microbial action, which comprises the following steps: collecting soil impedance and microbial indexes, correcting microbial distribution by using impedance depth, and executing environmental inhibition attenuation on nitrogen fixation and phosphorus solubilization rate by combining a carbon source and acid-base buffer capacity. And quantifying biological available nutrient supply, and generating a fertilizer discharge signal based on a crop demand gap and mechanical parameters. According to the method, the vertical cone index and the microbial gene abundance are collected, physical impedance data are introduced to correct the microbial spatial distribution weight, the metabolic activation model is constructed in combination with active organic carbon and the acid-base buffer capacity, and the actual nutrient conversion efficiency and the effective fertilizer supply total amount of nitrogen-fixing bacteria and phosphate solubilizing bacteria are quantified; biological fertilizer supply capacity is brought into a nutrient income and expenditure balance system, a discrete fertilizer discharge control instruction is generated according to actual gaps of crops and mechanical operation parameters, and the utilization rate of chemical fertilizer and agricultural ecological benefits are improved.

This invention relates to the field of microbial genetic engineering technology, specifically to a recombinant vector for enhanced oil recovery (EOR), engineered bacterial strains for EOR, their construction methods and applications, engineered bacterial strain agents and their applications, and methods for crude oil recovery. The recombinant vector for EOR of this invention includes a starting vector and an expression gene integrated into the starting vector; the expression gene includes a surfactant synthesis gene and a heat-resistant gene; the surfactant synthesis gene is the srfA gene; the heat-resistant gene is selected from the hsp70 gene, hsp60 gene, or dnaK gene. In this invention, a dual-gene synergistic modification strategy is employed, simultaneously introducing a heat-resistant gene and a surfactant synthesis gene and optimizing their expression, thereby achieving a dual enhancement of the engineered strain's environmental adaptability and metabolic function.

Provided is a promoter, which has a nucleotide sequence shown in SEQ ID NO. 1. The promoter is obtained by means of rationally designing the promoter of a dapA gene, has obviously reduced transcriptional activity and can be used for the weakened expression of microorganism genes. Substituting the original promoters of the dapA gene, a ddh gene, an ilvA gene, a tdcB gene and a gltA gene in Corynebacterium glutamicum by using the promoter enables the expression levels of these genes to be significantly reduced, the carbon metabolic in a recombinant microorganism constructed thereby flows more to a threonine anabolism pathway, and the recombinant microorganism has a significantly improved production of threonine in comparison with an original strain and has good growth performance.

The invention belongs to the cross technical field of microbial genetic engineering and food fermentation production, and particularly relates to a vanillin degradation related gene, application and an engineering strain for producing vanillin. The gene for regulating and controlling the production of vanillin by microorganisms is a 3-ketoacyl-ACP reductase gene, and the base sequence of the gene is shown in SEQ ID NO: 1. The 3-ketoacyl-ACP reductase gene is applied to regulation and control of degradation of vanillin. And the 3-ketoacyl-ACP reductase gene is knocked out from the chassis strain to obtain the 3-ketoacyl-ACP reductase. According to the invention, the fact that the fabG gene participates in the conversion of vanillin into vanillic acid is found for the first time, then the fabG gene is further utilized to modify the strain, the modification mode is highly targeted and has a remarkable effect, and through directional knockout of fabG and further knockout of vdh genes, phdB, fabG and modABC genes, the degradation pathway of vanillin is effectively blocked, and the product loss is reduced.

The invention belongs to the technical field of microbial genetic engineering and protein delivery, and particularly relates to an engineered bacterial secretion system for efficiently delivering protein and application of the engineered bacterial secretion system. Specifically, on the basis of analyzing a delivery mechanism of bacteroides fragilis GS086 type double-effect protein T6SS, engineering modification is performed on a toxic structural domain of the effect protein, and a bacterial secretion system capable of efficiently and simultaneously delivering a plurality of proteins is constructed. According to the system, a modified strain is a natural human intestinal symbiotic bacterium, the problems of high immunogenicity, poor stability and the like are effectively avoided, and protein can be delivered in situ in the intestinal tract to realize precise regulation and control of an intestinal flora structure and secretion of specific target protein, so that the system has a good practical application value.

The application discloses a method for optimizing protein translation extension process to improve protein yield of saccharomyces cerevisiae, and belongs to the technical field of microbial genetic engineering. The application improves the global protein synthesis capacity of saccharomyces cerevisiae by regulating the translation elongation factor in the translation initiation process, so that the protein content of saccharomyces cerevisiae is increased from 45.63 g / 100 g to 48.06 g / 100 g, and the protein yield is increased from 3.23 g / L to 3.46 g / L. The application provides technical support for low-cost and green single-cell protein production, and has a wide application prospect in the field of protein substitution.

Genetically engineered bacteria which express RNAs or proteins that produce or represent agriculturally useful compounds including fertilizers and plant nutrients upon increases in cell density when grown in plant growth media such as soil are disclosed. Also disclosed are genetically engineered bacteria where expression of RNAs or proteins that produce or represent agriculturally useful compounds including fertilizers and plant nutrients can be inhibited upon reaching increased cell densities when grown in a fermenter or bioreactor.

The present invention relates to compositions and methods for preventing or treating diseases or disorders involving nucleic acid damage, such as cancer, in a subject, as well as methods of identifying gut microbes, strains or metabolites thereof associated with nucleic acid damage and diseases or disorders involving nucleic acid damage, such as cancer.

The invention discloses a bottled beverage detection method, device and equipment and a medium, and relates to the technical field of artificial intelligence. Comprising the steps of obtaining metal impurity data and weight data of a beverage sample based on a preset sensor; scanning the beverage sample based on a preset spectrum technology to obtain corresponding spectrum data, and determining component data of the beverage sample based on the spectrum data by using a preset artificial intelligence algorithm; the method comprises the following steps: amplifying and detecting a target microorganism gene segment in a beverage sample by using a fluorescent quantitative PCR instrument to determine whether a target bacterium exists in the beverage sample, and performing specific binding with the target bacterium by using a preset biological recognition element to obtain bacterium detection data of the beverage sample; and analyzing and processing the metal impurity data, the weight data, the component data and the fungus detection data by using a preset artificial intelligence algorithm to obtain a detection result of the beverage sample. Therefore, appearance defects, microbial contamination or foreign matter doping of bottled beverages can be quickly identified.

This invention discloses a Bacillus bellis strain overexpressing a lipopeptide synthase gene, its construction method, and its application in fruit preservation. It belongs to the fields of microbial genetic engineering and postharvest fruit preservation technology. Using Bacillus bellis QB250122 as the starting strain, two rounds of genome editing were performed using a dual-plasmid temperature-sensitive CRISPR-Cas9n system. In the first round, the srfA promoter was replaced with the PHpaII superpromoter. In the second round, a complete expression cassette was inserted at the bacA site. The engineered strain produced 900-1200 mg / L of surfactantin. Applying a bacterial suspension to the surface of fruits such as mangoes can extend shelf life by 10-15 days.

This invention provides methods, computer programs, computer-readable storage media, and systems that offer improved solutions for processing genetic data. [Solution] The processing method comprises the steps of: S10 obtaining a dataset containing microbial gene expression data of multiple patients; S20 clustering multiple patients into a set of clusters based on the microbial gene expression data; and S50 determining genes exhibiting expression that explains the clustering by selecting genes included in a set of metabolic pathways and / or identifying genes that show significantly different expression between clusters, thereby forming an improved solution for patient stratification based on metagenomic data.

The invention provides a talaromyces pinophilus engineering bacterium and application thereof, and belongs to the technical field of microbial genetic engineering. The invention provides an application of a reagent for knocking out a 0585 gene or a coding product of the 0585 gene in reducing the yield of a talaromyces pinophilus Li-93 spore. The invention further provides a preparation method of the talaromyces pinophilus Li-93 spore. In the application of the talaromyces pinophilus Li-93A mutant, the talaromyces pinophilus Li-93A mutant can be obtained by knocking out the 0585 gene or an encoding product of the 0585 gene, the talaromyces pinophilus Li-93A mutant and an original strain have obvious phenotypic difference, the sporulation quantity is greatly reduced, but the capacity of the talaromyces pinophilus Li-93A mutant for producing beta-glucuronidase (TpGUS) is not influenced. The low-spore characteristic solves the pollution, allergy and safety problems caused by spores in industrial fermentation, and has unexpected biosafety and production convenience advantages.

The invention provides a mutant of phenylalanine tRNA (transfer ribonucleic acid) alpha subunit synthetase mPheS of rhinotrichum gloeosporioides, which has T257A and A304G double-site mutation relative to a wild type amino acid sequence, the invention further provides a coding gene of the mutant, and the gene of the mutant is applied to microbial gene knockout. An efficient gene traceless knockout strategy of the rhinotrichum-like is established, and efficient gene traceless knockout of the rhinotrichum-like can be achieved.

The invention belongs to the technical field of microbiological omics online analysis, and discloses a microbiological omics online analysis platform architecture of genomics and bioinformatics. The microbiomics online analysis platform architecture of genomics and bioinformatics comprises a microbiological information acquisition module, an information transmission module, a central control module, a microbiological gene sequencing module, a microbiological gene analysis module, a species judgment module, an information storage module and an interactive display module. The method comprises the following steps: obtaining a target microbial gene sample sequence corresponding to an initial target microbial gene sample through a microbial gene sequencing module; a methylation site in the target microbial gene sample sequence is determined, and an accurate target microbial gene sequencing result is obtained; meanwhile, correction parameters are adopted through the microbial gene analysis module, so that a data source with a large error is prevented from being used for calculation, an analysis result has a high reference value, and the accuracy of microbial gene analysis is improved.

The invention belongs to the technical field of biocontrol microorganism genetic engineering, and particularly relates to an sfgA gene for regulating and controlling growth and development of beauveria bassiana and application of the sfgA gene. The invention discloses an sfgA gene for regulating and controlling growth and development of beauveria bassiana, the nucleotide sequence of the sfgA gene is as shown in SEQ ID NO.1, and the amino acid sequence of encoded protein of the sfgA gene is as shown in SEQ ID NO.2. By systematically researching the effect of the development signal protein sfgA in the asexual spore production process of the beauveria bassiana and the regulation and control mechanism of the development signal protein sfgA, the growth and development of the beauveria bassiana can be regulated and controlled. A theoretical basis is laid for researching a filamentous fungus asexual sporulation molecular mechanism, and a new scientific basis and gene resources are provided for mining and genetic improvement of biocontrol potentials of biocontrol fungi.

The invention provides a DNA molecule for coding feruloyl esterase, a recombinant expression vector, a genetically engineered bacterium and application of the genetically engineered bacterium in production of feruloyl esterase, and belongs to the technical field of microbial genetic engineering. The nucleotide sequence of the DNA molecule for coding the feruloyl esterase is as shown in SEQ ID No. 1, and the amino acid sequence correspondingly coded by the DNA molecule is as shown in SEQ ID No. 2. The invention also discloses a recombinant expression vector and a genetically engineered bacterium which comprise the DNA molecule for coding the feruloyl esterase. According to the feruloyl esterase BccFae3074, a strain extracted from soil is used for sequencing, then a corresponding expression vector and engineering bacteria are obtained through genetic engineering modification, and the produced feruloyl esterase has relatively high enzymatic activity and yield; by optimizing the selection of carriers and the optimization of induction conditions, large-scale fermentation and industrial production are realized, ferulic acid is released through enzymolysis, high-value utilization of wastes such as straws is promoted, and pollution is reduced.