Bacillus thuringiensis nematocide crystal protein gene cry1518-35 and uses thereof

Abstract

The invention belongs to the technical field of microorganism genetic engineering and discloses separation and cloning of nematicidal crystal protein gene originated from thuringiensis and biological activity of the nematicidal crystal protein gene on nematode. In the invention, a new insecticidal crystal protein gene cry1518-35 is obtained by being separated from bacterial strain of activated thuringiensis YBT-1518 of root-knot nematodes in north of China; an encoded product of the gene is a new insecticidal crystal protein Cry1518-35; the protein Cry1518-35 features high insecticidal activity on root-knot nematodes in north of China. The invention also includes the preparation of colon bacillus recombinant bacteria EMB0225 which expresses the insecticidal crystal protein Cry1518-35, the recombinant colon bacillus is preserved in CCTCC, the preservation number is CCTCC NO: M209009.

Description

technical field [0001] The invention belongs to the fields of agricultural biological control and agricultural microorganism genetic engineering. It specifically relates to the isolation and cloning of an insecticidal crystal protein gene with nematicide activity and the application of its encoded protein in microbial insecticides. Background technique [0002] Bacillus thuringiensis (Bt) is a kind of Gram-positive bacteria that widely exists in soil. During its growth and development, it can form endophytic spores, and it is accompanied by the formation of spores to produce specific toxicity to insects. Insecticidal Crystal Proteins (ICPs) are different from Bacillus cereus which secretes enterotoxin and Bacillus anthracis which causes anthrax. Studies have shown that the crystal protein produced by Bacillus thuringiensis can treat more than 500 kinds of insects in 10 orders of Insecta, such as Lepidoptera, Diptera, Coleoptera, Hymenoptera, and Homoptera, as well as protoz...

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